CN104350954A - Lyophyllum decastes cultivation method - Google Patents

Lyophyllum decastes cultivation method Download PDF

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Publication number
CN104350954A
CN104350954A CN201410700135.9A CN201410700135A CN104350954A CN 104350954 A CN104350954 A CN 104350954A CN 201410700135 A CN201410700135 A CN 201410700135A CN 104350954 A CN104350954 A CN 104350954A
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days
day
bottle
mycelium stimulation
illumination
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CN104350954B (en
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贲伟东
杨仁智
向银春
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JIANGSU GUBENTANG BIOLOGICAL TECHNOLOGY Co Ltd
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JIANGSU GUBENTANG BIOLOGICAL TECHNOLOGY Co Ltd
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G18/00Cultivation of mushrooms
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G5/00Fertilisers characterised by their form
    • C05G5/40Fertilisers incorporated into a matrix

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  • Life Sciences & Earth Sciences (AREA)
  • Pest Control & Pesticides (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Mycology (AREA)
  • Environmental Sciences (AREA)
  • Mushroom Cultivation (AREA)

Abstract

The invention relates to a lyophyllum decastes cultivation method. The lyophyllum decastess cultivation method comprises the following steps: weighing culture medium raw materials in parts by mass; mixing the raw materials; controlling the pH value to be 6-7 and putting the materials into culture bottles, wherein 400-500 parts of the raw materials are put into each bottle; pressing a cap and sealing; conveying the culture bottle into a vacuum sterilization pot; sterilizing with ozone for 1 to hours until the vacuumizing degree is 50KPa-80KPa; sterilizing at 150 DEG C for 100-120 minutes; cooling at 15-20 DEG C and adding 1-4 parts of humic acid; cooling and conveying a mixture into each bottle to be inoculated for 40-50 minutes; culturing in a culture room at 20-25 DEG C for 20-25 days under the conditions that the humidity is 70%-80% and the concentration of carbon dioxide is 1600PPM-3200PPM; carrying out mycelium stimulation: carrying out mycelium stimulation treatment by using a mycelium stimulation machine and supplying water according to 85% of the weight of old mycoderma; carrying out bud promotion and collecting: after the mycelium stimulation, starting to collect after 21 days in the culture room under the conditions that the temperature is 14-15 DEG C, the humidity is 95%-98%, the concentration of the carbon dioxide is under 1200PPM, the illumination intensity of 0-50Lux from day 1 to day 3, the illumination intensity of 50Lux-100Lux from day 4 to day 6, the illumination intensity of 150Lux-200Lux from day 7 to day10 days, and the illumination intensity of 200Lux-300Lux for day 10 to day 20.

Description

Pilose antler mushroom cultivation method
Technical field
The present invention relates to a kind of pilose antler mushroom cultivation method.
Background technology
Pilose antler mushroom is also known as Lyophyllum decastes (Lyophyllum decastes (Fr.: Fr.) Sing.), and lotus leaf mushroom, is also called cold fragrant bacterium in the Yunnan of China, a brood of sheep, Fungus Pleurotus ostreatus etc., belong to Basidiomycotina, Hymenomycetes, cap order, Bai Mo section, from Agaricus, be called fried chicken mushroom in Europe, bacterial context is plump, fine and smooth, A sweety scent assails the nostrils, delicious flavour, research shows that crude protein in pilose antler massee fruiting bodies, amino acid whose content are higher, and fat content is lower; But also contain useful trace element zinc, copper and the selenium of human body and a large amount of vitamin B1s, B2, B6, B12 and nicotinic acid, there is very high nutritive value.Show according to modern scientific research, the beta glucan that pilose antler mushroom contains, long-term eating has antineoplastic effect, meanwhile, pilose antler mushroom have hypotensive, reduce the pharmacologic action such as cholesterol, anti-diabetic, antiallergy, can strengthening by means of tonics, strengthen the body resistance to consolidate the constitution, strengthen immunity, delay senility.
The domestic research for the cultivation of pilose antler mushroom at present concentrates in the research of biological character mostly, and less to cultivation research, within 2007, Shanghai Finc Bio-tech Inc. reports industrialized cultivation pilose antler mushroom first.This article is pointed out to guarantee that the low stain rate that factory culture brings benefits is that cultivation is successfully crucial simultaneously.The link of Environmental capacity most critical is that bacterial classification can realize surely growing fast on composts or fertilisers of cultivating, and the vigor of bacterial classification is then surely grow most important factor fast.Inner Mongolia Autonomous Region in 2008 Yakeshi City Yi Tuli river State Forestry Administration, P.R. China feels that Lyophyllum decastes (pilose antler mushroom) cultivation method patent has been applied in careless Xun forest farm, adopt solid spawn planting type, solid spawn preparation cost is long, and inoculation efficiency is low, bacterial classification germination physiology is slow, and the pollution rate of blake bottle is high.
Summary of the invention
The invention provides a kind of preparation cost low, inoculation efficiency, bacterial classification germination physiology, reduce the pilose antler mushroom liquid strain preparation method of blake bottle pollution rate.
The technical solution used in the present invention is: a kind of pilose antler mushroom cultivation method, is characterized in that:
(1), by mass fraction culture medium raw material is taken: wood chip 40-60 part, rice bran 10-20 part, wheat bran 5-10 part, continuous seed shell 10-20 part, lime 1-3 part, Nutrition Soil 10-15 part, water 80-110 part;
(2), by step (1) raw material mix, control pH value 6-7, load blake bottle, every bottle of 400-500 part, gland encapsulates;
(3), blake bottle sends into vacreation pot, suction 50-80KPa after ozone sterilization 1-2 hour, sterilization 100-120 minute at 150 DEG C;
(4), at 15-20 DEG C cool, add humus 1-4 part, after cooling, send into every bottle graft original seed 40-50;
(5), 20-25 DEG C of culturing room, humidity 70-80%, between gas concentration lwevel 1600-3200PPM, cultivates 20-25 days;
(6), mycelium stimulation process, utilize mushroom culturing device to carry out mycelium stimulation process and carry out moisturizing by 85% of old mycoderma weight simultaneously;
(7) flower bud process, is urged, in culturing room after mycelium stimulation, temperature 14-15 DEG C, humidity 95-98%, below gas concentration lwevel 1200PPM, 1-3 days intensity of illumination 0-50Lux, 4-6 days intensity of illumination 50-100Lux, 7-10 days intensity of illumination 150-200Lux, 10-20 days intensity of illumination 200-300Lux, started to gather after 21 days.
Compared with prior art, advantage of the present invention is: medium is easy to make, cost is low, and when making for liquid spawn, it is fast that bacterial classification sends out bacterium speed, pollution-free, without old mycoderma, is conducive to improving and sends out bacterium effect and output.Add humus, effectively improve rate of buddingging, guarantee bacterium mushroom output.
Embodiment
Below in conjunction with embodiment, the invention will be further described.
Embodiment one
A kind of pilose antler mushroom liquid strain preparation method:
(1), by mass fraction culture medium raw material is taken: wood chip 40 parts, 10 parts, rice bran, 5 parts, wheat bran, 10 parts, continuous seed shell, 1 part, lime, Nutrition Soil 10 parts, 80 parts, water;
(2), by step (1) raw material mix, control pH value 6, load blake bottle, 400 parts every bottle, gland encapsulates;
(3), blake bottle sends into vacreation pot, and ozone sterilization is suction 50KPa after 1 hour, sterilization 100 minutes at 150 DEG C;
(4), at 15 DEG C cool, add humus 1 part, after cooling, send into every bottle graft original seed 40;
(5), 20 DEG C of culturing room, humidity 70%, between gas concentration lwevel 1600PPM, cultivates 20 days;
(6), mycelium stimulation process, utilize mushroom culturing device to carry out mycelium stimulation process and carry out moisturizing by 85% of old mycoderma weight simultaneously;
(7), flower bud is urged to gather, in culturing room after mycelium stimulation, temperature 14 DEG C, humidity 95%, below gas concentration lwevel 1200PPM, 1-3 days intensity of illumination 0-50Lux, 4-6 days intensity of illumination 50-100Lux, 7-10 days intensity of illumination 150-200Lux, 10-20 days intensity of illumination 200-300Lux, started to gather after 21 days.
Embodiment two
A kind of pilose antler mushroom liquid strain preparation method:
(1), by mass fraction culture medium raw material is taken: wood chip 60 parts, 20 parts, rice bran, 10 parts, wheat bran, 20 parts, continuous seed shell, 3 parts, lime, Nutrition Soil 15 parts, 110 parts, water;
(2), by step (1) raw material mix, control pH value 7, load blake bottle, 500 parts every bottle, gland encapsulates;
(3), blake bottle sends into vacreation pot, and ozone sterilization is suction 80KPa after 2 hours, sterilization 120 minutes at 150 DEG C;
(4), at 20 DEG C cool, add humus 2 parts, after cooling, send into every bottle graft original seed 50;
(5), 25 DEG C of culturing room, humidity 80%, between gas concentration lwevel 3200PPM, cultivates 25 days;
(6), mycelium stimulation process, utilize mushroom culturing device to carry out mycelium stimulation process and carry out moisturizing by 85% of old mycoderma weight simultaneously;
(7), flower bud is urged to gather, in culturing room after mycelium stimulation, temperature 15 DEG C, humidity 98%, below gas concentration lwevel 1200PPM, 1-3 days intensity of illumination 0-50Lux, 4-6 days intensity of illumination 50-100Lux, 7-10 days intensity of illumination 150-200Lux, 10-20 days intensity of illumination 200-300Lux, started to gather after 21 days.
 
Embodiment three
A kind of pilose antler mushroom liquid strain preparation method:
(1), by mass fraction culture medium raw material is taken: wood chip 50 parts, 15 parts, rice bran, 8 parts, wheat bran, 15 parts, continuous seed shell, 2 parts, lime, Nutrition Soil 13 parts, 90 parts, water;
(2), by step (1) raw material mix, control pH value 6, load blake bottle, 450 parts every bottle, gland encapsulates;
(3), blake bottle sends into vacreation pot, and ozone sterilization is suction 60KPa after 1.5 hours, sterilization 110 minutes at 150 DEG C;
(4), at 18 DEG C cool, add humus 4 parts, after cooling, send into every bottle graft original seed 46;
(5), 22 DEG C of culturing room, humidity 78%, between gas concentration lwevel 2200PPM, cultivates 22 days;
(6), mycelium stimulation process, utilize mushroom culturing device to carry out mycelium stimulation process and carry out moisturizing by 85% of old mycoderma weight simultaneously;
(7), flower bud is urged to gather, in culturing room after mycelium stimulation, temperature 14 DEG C, humidity 98%, below gas concentration lwevel 1200PPM, 1-3 days intensity of illumination 0-50Lux, 4-6 days intensity of illumination 50-100Lux, 7-10 days intensity of illumination 150-200Lux, 10-20 days intensity of illumination 200-300Lux, started to gather after 21 days.

Claims (1)

1. a pilose antler mushroom cultivation method, is characterized in that:
(1), by mass fraction culture medium raw material is taken: wood chip 40-60 part, rice bran 10-20 part, wheat bran 5-10 part, continuous seed shell 10-20 part, lime 1-3 part, Nutrition Soil 10-15 part, water 80-110 part;
(2), by step (1) raw material mix, control pH value 6-7, load blake bottle, every bottle of 400-500 part, gland encapsulates;
(3), blake bottle sends into vacreation pot, suction 50-80KPa after ozone sterilization 1-2 hour, sterilization 100-120 minute at 150 DEG C;
(4), at 15-20 DEG C cool, add humus 1-4 part, after cooling, send into every bottle graft original seed 40-50;
(5), 20-25 DEG C of culturing room, humidity 70-80%, between gas concentration lwevel 1600-3200PPM, cultivates 20-25 days;
(6), mycelium stimulation process, utilize mushroom culturing device to carry out mycelium stimulation process and carry out moisturizing by 85% of old mycoderma weight simultaneously;
(7), flower bud is urged to gather, in culturing room after mycelium stimulation, temperature 14-15 DEG C, humidity 95-98%, below gas concentration lwevel 1200PPM, 1-3 days intensity of illumination 0-50Lux, 4-6 days intensity of illumination 50-100Lux, 7-10 days intensity of illumination 150-200Lux, 10-20 days intensity of illumination 200-300Lux, started to gather after 21 days.
CN201410700135.9A 2014-02-22 2014-11-28 Cornu Cervi Pantotrichum mushroom cultivation method Active CN104350954B (en)

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Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105218197A (en) * 2015-11-02 2016-01-06 江苏农牧科技职业学院 A kind of pilose antler mushroom cultivation medium formula and preparation method thereof
CN109548561A (en) * 2018-12-25 2019-04-02 昆山青禾食用菌科技有限公司 A kind of pilose antler mushroom culture method
CN110663453A (en) * 2019-11-15 2020-01-10 昆山青禾食用菌科技有限公司 Lyophyllum decastes culture material, preparation method and application
CN111296173A (en) * 2020-03-27 2020-06-19 江苏华绿生物科技股份有限公司 Industrialized cultivation medium for velvet antler mushroom and method for cultivating edible fungi
CN111512885A (en) * 2020-04-28 2020-08-11 江苏华绿生物科技股份有限公司 Preparation process for industrially cultivating velvet antler mushroom
CN111771616A (en) * 2020-08-18 2020-10-16 湖南和平生物科技有限公司 Industrialized cultivation method of velvet antler mushroom
CN113412760A (en) * 2021-06-22 2021-09-21 江苏华绿生物科技股份有限公司 Hypsizigus marmoreus culture medium and hypsizigus marmoreus cultivation process
CN113796263A (en) * 2021-09-13 2021-12-17 贵州大秦农业科技有限公司 Large-scale cultivation method of velvet antler mushroom
CN116171797A (en) * 2023-04-04 2023-05-30 福建恒绿生物科技有限公司 Cultivation method of velvet mushrooms

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2001025320A (en) * 1990-03-08 2001-01-30 Takara Shuzo Co Ltd Artificial culture of lyophyllum decastes
JP2007054044A (en) * 2005-07-26 2007-03-08 Yamasa Shoyu Co Ltd Method for artificially culturing lyophyllum shimeji and culture medium
CN103210849A (en) * 2013-03-04 2013-07-24 上海丰科生物科技股份有限公司 Lyophyllum decastes new bacterial strain
CN103340156A (en) * 2013-03-25 2013-10-09 上海丰科生物科技股份有限公司 Novel strain of lyophyllum decastes

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2001025320A (en) * 1990-03-08 2001-01-30 Takara Shuzo Co Ltd Artificial culture of lyophyllum decastes
JP2007054044A (en) * 2005-07-26 2007-03-08 Yamasa Shoyu Co Ltd Method for artificially culturing lyophyllum shimeji and culture medium
CN103210849A (en) * 2013-03-04 2013-07-24 上海丰科生物科技股份有限公司 Lyophyllum decastes new bacterial strain
CN103340156A (en) * 2013-03-25 2013-10-09 上海丰科生物科技股份有限公司 Novel strain of lyophyllum decastes

Cited By (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105218197A (en) * 2015-11-02 2016-01-06 江苏农牧科技职业学院 A kind of pilose antler mushroom cultivation medium formula and preparation method thereof
CN105218197B (en) * 2015-11-02 2018-07-13 江苏农牧科技职业学院 A kind of pilose antler mushroom cultivation medium formula and preparation method thereof
CN109548561A (en) * 2018-12-25 2019-04-02 昆山青禾食用菌科技有限公司 A kind of pilose antler mushroom culture method
CN110663453A (en) * 2019-11-15 2020-01-10 昆山青禾食用菌科技有限公司 Lyophyllum decastes culture material, preparation method and application
CN111296173A (en) * 2020-03-27 2020-06-19 江苏华绿生物科技股份有限公司 Industrialized cultivation medium for velvet antler mushroom and method for cultivating edible fungi
CN111512885A (en) * 2020-04-28 2020-08-11 江苏华绿生物科技股份有限公司 Preparation process for industrially cultivating velvet antler mushroom
CN111771616A (en) * 2020-08-18 2020-10-16 湖南和平生物科技有限公司 Industrialized cultivation method of velvet antler mushroom
CN113412760A (en) * 2021-06-22 2021-09-21 江苏华绿生物科技股份有限公司 Hypsizigus marmoreus culture medium and hypsizigus marmoreus cultivation process
CN113796263A (en) * 2021-09-13 2021-12-17 贵州大秦农业科技有限公司 Large-scale cultivation method of velvet antler mushroom
CN116171797A (en) * 2023-04-04 2023-05-30 福建恒绿生物科技有限公司 Cultivation method of velvet mushrooms

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