CN103960184A - Breeding method for increasing survival rate of loaches manually bred in factory-like mode - Google Patents
Breeding method for increasing survival rate of loaches manually bred in factory-like mode Download PDFInfo
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Abstract
The invention belongs to the technical field of aquaculture, and particularly relates to a breeding method for increasing the survival rate of loaches manually bred in a factory-like mode, wherein the breeding method is reasonable, simple and high in operability. According to the breeding method, the natural mating method, the sperm extrusion method and the laparotomy spermatophore-obtaining method can be adopted, the death rate of parent fish for breeding can be greatly reduced, and the spawning induction rate, the fertilization rate and the hatching rate can be increased.
Description
Technical field
The invention belongs to technical field of aquaculture, relate in particular to a kind of seedling-cultivating method of loach industrial artificial breeding raising survival rate.
Background technology
Loach fat-containing component is lower, cholesterol still less, high protein and low fat food, containing a kind of unsaturated fatty acid of similar twenty carbon penetenoic acid, the anti-angiogenic aging of favourable human body, is of value to the elderly and cardiovascular patient.There is tonifying middle-Jiao and Qi, dehumidifying removing jaundice, kidney-nourishing is supporing yang, eliminating dampness to stop diarrhea, warming spleen and stomach, treatment hemorrhoid, effect of abnormal sweating only.Be called as " ginseng in water ".
Loach breeding scale develops rapidly in recent years, and Miao ethnic group becomes the bottleneck of restriction industry development.At present, the seed of China's loach breeding is mainly the wild[l of manually fishing for, and due to pollutions such as chemical fertilizer, agricultural chemicals, three industrial wastes, causes loach wild resource to fall sharply, and wild seed resource can not meet the needs of large-scale cultivation.How to carry out biological control, improve fertilization rate and the incubation rate of loach ovum.Seed rearing survival rate, reducing labour intensity and reproductive-cost is its key point.
If Chinese Patent Application No. is a kind of loach artificial propagation method for advanced fry of " 201310074705.3 ", this method has all reduced the lethality of breeding parent population to a certain extent, has improved spawning rate, fertilization rate, incubation rate.But the hatching pail that the method is used is diameter 15cm, the cylinder of high 40cm, this kind of hatching pail water consumption is large, has hatching dead angle simultaneously, and incubation rate is not high.And it was not carried out to unsticking operation before fertilized egg, and easily cause fertilized egg adhesion, reduce the incubation rate of fertilized egg.
Summary of the invention
The present invention is directed to the deficiency of existing loach seedling propagation technique, rationally simple, strong operability of a kind of method is provided, loach industrial artificial breeding that can large-scale cultivation improves the seedling-cultivating method of survival rate.The method can reduce the lethality of breeding parent population greatly, improves spawning rate, fertilization rate, incubation rate.
Above-mentioned technical problem of the present invention is implemented by the following technical programs:
The breeding of loach industrial artificial improves a seedling-cultivating method for survival rate, it is characterized in that, comprises the following steps:
A. parent selects
Select physical health, color and luster is normal, movable strong parent, the feeding water earthworm of 3 ~ 5 days;
B. artificial induced spawning
At 24 ~ 28 DEG C of water temperatures, female parent is carried out to artificial induced spawning, injectable drug is luteinizing hormone releasing hormone A
2, Pan Li ketone, physiological saline, adopt intramuscular injection;
C. artificial insemination
Natural mating method: the female male parent of having hastened parturition is put into the net cage of laying eggs in 2 ﹕ 1 ratios, then put into all smooth cement pits of five faces, treat its natural spawning fertilization;
D. incubating oosperm
After fertilization, by fertilized egg unsticking, viscidity-removing method is: in fertilized egg, add talcum powder in the ratio of Hua Shi Fen ﹕ fertilized egg=1 ﹕ 3, then after the water that adds 1～2 times stirs, with 80 mesh sieves filtrations, wash away after talcum powder, fertilized egg is put into taper hatching pail and hatch, each taper hatching pail can be hatched 5,000,000～7,000,000 loach seedlings, and hatching water temperature requires at 23 ~ 28 DEG C;
E. loach seedling is raised
After 48 hours, spray scatters in pond, while starting to move about food finding, just can feed loach seedling open-mouthed bait, feeding quantity is calculated by 0.5% of loach seedling gross weight, and feeding method is: open-mouthed bait is placed in plate, put in every square metre of ratio of putting a dish, after two hours, take out plate, from feeding first day, will change water every day, changing water method is: first bleed off in cement pit 1/3rd Chi Shui, then injecting clear water; In the time that loach seedling grows to 2cm, cut off open-mouthed bait, make live body silk earthworm feeding into, still to change water every day, cement pit seedling density will be controlled at 3000～5000 tails/m
2;
F. loach seedling maturation
When after 12 days, loach seedling has grown up to 3cm, and change can be put loach seedling in a suitable place to breed in the large pool, also can start to sell as seed; Large pool cultivation 15～20 days, loach seedling can grow up to 6～8cm, until become commodity loach, also can in cement pit, cultivate commodity loach and sell.
As preferably, injecting method concrete in described step B is: on operating desk, pad the cotton felt that 0.5 ~ 1cm is thick, separately do the circle of a 15cm diameter, upper seam gauze, in order to cover the loach parent who is placed on cotton felt, adopt the mode of intramuscular injection that oxytocins is expelled in loach body.
As preferably, the processing method of described loach seedling open-mouthed bait is: 60% earthworm is worn into slurry and the 10% new fresh milk that squeezes mixes, add the white young material of 30% eel stir alive agglomerating then make little granular.
As preferably, the mesh of the net cage of laying eggs in described step B is 9 orders, the thick 1mm of nettle, and the large 2mm in hole, specification is long 2m × wide 1m × high 0.5m.The net cage of laying eggs can prevent that loach parent from eating ovum.
As preferably, the back cut diameter of described taper hatching pail is 1 meter, and 0.2 meter of lower port diameter is high 1 meter.
As preferably, the water in described step C is underground water, pH=6.5 ~ 7 of water, and the dissolved oxygen amount of water is 8mg/L at least.
As preferably, five described smooth cement pit degree of depth are 70 ~ 90cm.
As preferably, described fertilization method can also be crowded smart method: the seminal fluid of manually extruding a male loach, then add 0.2%～0.3% normal saline dilution, the consumption of the physiological saline adding be the ovum that produces of 3 female loach volume 1/3rd, then seminal fluid is poured in the ovum of 3 female loach products, and stir with goose feather, it is evenly fertilized.The feritilization of ovum that adopts the sperm of extruding while squeezing smart method to produce with 3 female loach.
As preferably, described fertilization method can also be got seminal vesicle method for cuing open: get a public loach, cut belly open, take out seminal vesicle and extrude seminal fluid, then add 0.2%～0.3% normal saline dilution, the consumption of the physiological saline adding be the ovum that produces of 10 female loach volume 1/3rd, then seminal fluid is poured in the ovum that 10 female loach produce, and stir with goose feather, it is evenly fertilized.The feritilization of ovum that the sperm extruded while getting seminal vesicle method can be produced with 10 female loach is cutd open in employing.
The present invention compared with prior art, has following advantage.
1. what this method mainly adopted is the method for artificial insemination, can be natural mating method, squeeze smart method, can also be to cut open the belly to get seminal vesicle method, adopt natural mating method, male parent can survive and continue to raise, protection parent that can be best is injury-free, adopt the smart method of squeezing, male parent also can also continue to survive several days, in in the past few days, male parent is sold as the loach that lives, also can obtain higher economic worth, get seminal vesicle method and adopt to cut open the belly, although can obtain the sperm of maximum, but male parent will be dead, can only work as the loach of seeking death sells, economic worth is lower.
2. when this method is given female male parent oxytocin injection, made specific purpose tool by oneself, prevented from directly grabbing the female male parent of damage with hand, improve the spawning rate of female male parent, and parent's lethality also can be in 2%, parent's lethality has reduced by 13%.
3. the present invention is to 0.2%～0.3% normal saline dilution for the sperm taking out, and its object is to activate sperm, increase sperm viability.
4. the water of using in this method is underground water, pH=6.5 ~ 7 of water, and the dissolved oxygen amount of water is 8mg/L at least.The content of microorganisms of underground water is low, and the content of hydrogen sulphide, nitrogen oxide is also lower, is more conducive to the growth of seedling.
5. cement pit five faces in the present invention are all smooth, are beneficial to cleanly, remove the pest in pond, can better control pH value.
6. the benefit that adopts taper hatching pail to have has: using water wisely, than the water consumption of conventional incubation case hatching saving 2/3rds; The dissolved oxygen amount that increases water, makes the dissolved oxygen amount of water all the time higher than 8mg/L; Increased hatching density, a taper hatching pail is better than tens 20m
2the hatching amount in pond, a traditional 20m
2the parent of hatchery Ke Fang two teams hatch 1,000,000 left and right loach seedlings, and a hatching pail just can hatch 500～7,000,000 loach seedlings, and the occupation of land of a hatching pail only has 1m
2, therefore save greatly land used, improve the incubation rate of unit are; Taper hatching pail is compared and columniform hatching pail, has reduced dead angle, increases the floating momentum of incubating, and improves incubation rate.
Be more than 98% by parent's survival rate after this mating system, improved 13%, spawning rate is more than 98%, has improved 7%, and fertilization rate more than 98.5%, has improved 1%, and incubation rate more than 99.2%, has improved 7.8%, and seedling percent is more than 85%, to have improved 65%.
Embodiment
Below by embodiment, technical scheme of the present invention is described in further detail:
Embodiment 1: a kind of loach industrial artificial breeding improves the seedling-cultivating method of survival rate, comprises the following steps:
A. parent selects
Select physical health, color and luster is normal, movable strong parent, the feeding water earthworm of 3 ~ 5 days;
B. artificial induced spawning
At 24 ~ 28 DEG C of water temperatures, female parent is carried out to artificial induced spawning, injectable drug is luteinizing hormone releasing hormone A
2125mg, Pan Li ketone (DOM) 100mg, physiological saline 80cc, three's hybrid injection, adopts intramuscular injection, must not be by infusion of medicine abdominal cavity, every endnote is penetrated 0.5cc; When injection, pad the cotton felt that 0.5 ~ 1cm is thick on operating desk, separately do the circle of a 15cm diameter, upper seam gauze, in order to cover the loach parent who is placed on cotton felt, keeps away manual-free and grabs, damage loach parent;
C. artificial insemination
Natural mating method: the female male parent of having hastened parturition is put into the net cage of laying eggs in 2 ﹕ 1 ratios, then put into all smooth cement pits of five faces, treat its natural spawning fertilization, the degree of depth of cement pit is 70 ~ 90cm, the mesh of net cage of laying eggs is 9 orders, the thick 1mm of nettle, the large 2mm in hole, specification is long 2m × wide 1m × high 0.5m;
D. incubating oosperm
After fertilization, by fertilized egg unsticking, viscidity-removing method is: in fertilized egg, add talcum powder in the ratio of Hua Shi Fen ﹕ fertilized egg=1 ﹕ 3, then after the water that adds 1～2 times stirs, with 80 mesh sieves filtrations, wash away after talcum powder, it is 1 meter that fertilized egg is put into back cut diameter, 0.2 meter of lower port diameter, hatching in the taper hatching pail of high 1 meter, each taper hatching pail can be hatched 5,000,000～7,000,000 loach seedlings, and hatching water temperature requires at 23 ~ 28 DEG C;
E. loach seedling is raised
After 48 hours, spray scatters in pond, while starting to move about food finding, just can feed loach seedling open-mouthed bait, feeding quantity is calculated by 0.5% of loach seedling gross weight, and feeding method is: open-mouthed bait is placed in plate, put in every square metre of ratio of putting a dish, after two hours, take out plate, from feeding first day, will change water every day, changing water method is: first bleed off in cement pit 1/3rd Chi Shui, then injecting clear water; In the time that loach seedling grows to 2cm, cut off open-mouthed bait, make live body silk earthworm feeding into, will change water every day, and cement pit seedling density will be controlled at 3000～5000 tails/m
2;
F. loach seedling maturation
When after 12 days, loach seedling has grown up to 3cm, and change can be put loach seedling in a suitable place to breed in the large pool, also can start to sell as seed; Large pool cultivation 15～20 days, loach seedling can grow up to 6～8cm, until become commodity loach, also can in cement pit, cultivate commodity loach and sell.
The processing method of described loach seedling open-mouthed bait is: 60% earthworm is worn into slurry and the 10% new fresh milk that squeezes mixes, add the white young material of 30% eel stir alive agglomerating then make little granular.
Water in cement pit is underground water, pH=6.5 ~ 7 of water, and the dissolved oxygen amount of water is 8mg/L at least.
Breed loach seedling by the method, can greatly reduce parent's lethality, improve the survival rate of seedling.Parent's survival rate is 100%, and spawning rate is 98%, fertilization rate 98.5%, and incubation rate 99.3%, seedling percent is 86%.
The method can realize the batch production of loach seedling and produce, for loach breeding provides a large amount of seeds, simultaneously can maximized protection parent not dead, has good economic benefit.
Embodiment 2: a kind of loach industrial artificial breeding improves the seedling-cultivating method of survival rate, comprises the following steps:
A. parent selects
Select physical health, color and luster is normal, movable strong parent, the feeding water earthworm of 3 ~ 5 days;
B. artificial induced spawning
At 24 ~ 28 DEG C of water temperatures, female parent is carried out to artificial induced spawning, injectable drug is luteinizing hormone releasing hormone A
2125mg, Pan Li ketone (DOM) 100mg, physiological saline 80cc, three's hybrid injection, adopts intramuscular injection, must not be by infusion of medicine abdominal cavity, every endnote is penetrated 0.5cc; When injection, pad the cotton felt that 0.5 ~ 1cm is thick on operating desk, separately do the circle of a 15cm diameter, upper seam gauze, in order to cover the loach parent who is placed on cotton felt, keeps away manual-free and grabs, damage loach parent;
C. artificial insemination
Squeeze smart method: the seminal fluid of manually extruding a male loach, then add 0.2%～0.3% normal saline dilution, the consumption of the physiological saline adding be the ovum that produces of 3 female loach volume 1/3rd, then seminal fluid is poured in the ovum that 3 female loach produce, and stir with goose feather, it is evenly fertilized;
D. incubating oosperm
After fertilization, by fertilized egg unsticking, viscidity-removing method is: in fertilized egg, add talcum powder in the ratio of Hua Shi Fen ﹕ fertilized egg=1 ﹕ 3, then after the water that adds 1～2 times stirs, with 80 mesh sieves filtrations, wash away after talcum powder, it is 1 meter that fertilized egg is put into back cut diameter, 0.2 meter of lower port diameter, hatching in the taper hatching pail of high 1 meter, each taper hatching pail can be hatched 5,000,000～7,000,000 loach seedlings, and hatching water temperature requires at 23 ~ 28 DEG C;
E. loach seedling is raised
After 48 hours, spray scatters in pond, while starting to move about food finding, just can feed loach seedling open-mouthed bait, feeding quantity is calculated by 0.5% of loach seedling gross weight, and feeding method is: open-mouthed bait is placed in plate, put in every square metre of ratio of putting a dish, after two hours, take out plate, from feeding first day, will change water every day, changing water method is: first bleed off in cement pit 1/3rd Chi Shui, then injecting clear water; In the time that loach seedling grows to 2cm, cut off open-mouthed bait, make live body silk earthworm feeding into, will change water every day, and cement pit seedling density will be controlled at 3000～5000 tails/m
2;
F. loach seedling maturation
When after 12 days, loach seedling has grown up to 3cm, and change can be put loach seedling in a suitable place to breed in the large pool, also can start to sell as seed; Large pool cultivation 15～20 days, loach seedling can grow up to 6～8cm, until become commodity loach, also can in cement pit, cultivate commodity loach and sell.
The processing method of described loach seedling open-mouthed bait is: 60% earthworm is worn into slurry and the 10% new fresh milk that squeezes mixes, add the white young material of 30% eel stir alive agglomerating then make little granular.
Water in cement pit is underground water, pH=6.5 ~ 7 of water, and the dissolved oxygen amount of water is 8mg/L at least.
Adopted and squeezed smart method by the method the time of fertilization, in can obtaining relatively large sperm, can make again parent survive, be beneficial to sale, ensured parent's economic worth, parent's survival rate is 100%.See on the whole, by this propagation method, spawning rate is 98.4%, fertilization rate 98.8%, and incubation rate 99.5%, seedling percent is 89%.
The method can realize the batch production of loach seedling and produce, and for loach breeding provides a large amount of seeds, has good economic benefit.
Embodiment 3: a kind of loach industrial artificial breeding improves the seedling-cultivating method of survival rate, comprises the following steps:
A. parent selects
Select physical health, color and luster is normal, movable strong parent, the feeding water earthworm of 3 ~ 5 days;
B. artificial induced spawning
At 24 ~ 28 DEG C of water temperatures, female parent is carried out to artificial induced spawning, injectable drug is luteinizing hormone releasing hormone A
2125mg, Pan Li ketone (DOM) 100mg, physiological saline 80cc, three's hybrid injection, adopts intramuscular injection, must not be by infusion of medicine abdominal cavity, every endnote is penetrated 0.5cc; When injection, pad the cotton felt that 0.5 ~ 1cm is thick on operating desk, separately do the circle of a 15cm diameter, upper seam gauze, in order to cover the loach parent who is placed on cotton felt, keeps away manual-free and grabs, damage loach parent;
C. artificial insemination
Cut open and get seminal vesicle method: get a public loach, cut belly open, take out seminal vesicle and extrude seminal fluid, then add 0.2%～0.3% normal saline dilution, the consumption of the physiological saline adding be the ovum that produces of 10 female loach volume 1/3rd, then seminal fluid is poured in the ovum that 10 female loach produce, and stirred with goose feather, it is evenly fertilized;
D. incubating oosperm
After fertilization, by fertilized egg unsticking, viscidity-removing method is: in fertilized egg, add talcum powder in the ratio of Hua Shi Fen ﹕ fertilized egg=1 ﹕ 3, then after the water that adds 1～2 times stirs, with 80 mesh sieves filtrations, wash away after talcum powder, it is 1 meter that fertilized egg is put into back cut diameter, 0.2 meter of lower port diameter, hatching in the taper hatching pail of high 1 meter, each taper hatching pail can be hatched 5,000,000～7,000,000 loach seedlings, and hatching water temperature requires at 23 ~ 28 DEG C;
E. loach seedling is raised
After 48 hours, spray scatters in pond, while starting to move about food finding, just can feed loach seedling open-mouthed bait, feeding quantity is calculated by 0.5% of loach seedling gross weight, and feeding method is: open-mouthed bait is placed in plate, put in every square metre of ratio of putting a dish, after two hours, take out plate, from feeding first day, will change water every day, changing water method is: first bleed off in cement pit 1/3rd Chi Shui, then injecting clear water; In the time that loach seedling grows to 2cm, cut off open-mouthed bait, make live body silk earthworm feeding into, will change water every day, and cement pit seedling density will be controlled at 3000～5000 tails/m
2;
F. loach seedling maturation
When after 12 days, loach seedling has grown up to 3cm, and change can be put loach seedling in a suitable place to breed in the large pool, also can start to sell as seed; Large pool cultivation 15～20 days, loach seedling can grow up to 6～8cm, until become commodity loach, also can in cement pit, cultivate commodity loach and sell.
The processing method of described loach seedling open-mouthed bait is: 60% earthworm is worn into slurry and the 10% new fresh milk that squeezes mixes, add the white young material of 30% eel stir alive agglomerating then make little granular.
Water in cement pit is underground water, pH=6.5 ~ 7 of water, and the dissolved oxygen amount of water is 8mg/L at least.
Breed loach seedling by the method, the acquisition sperm quantity of maximizing, but male parent is by death, can only process and sell as early as possible.Spawning rate is 100%, fertilization rate 99.5%, and incubation rate 99.5%, seedling percent is 90%.
The method can realize the batch production of loach seedling and produce, and for loach breeding provides a large amount of seeds, has good economic benefit.
Specific embodiment described herein is only to the explanation for example of the present invention's spirit.Those skilled in the art can make various amendments or supplement or adopt similar mode to substitute described specific embodiment, but can't depart from spirit of the present invention or surmount the defined scope of appended claims.
Claims (9)
1. the breeding of loach industrial artificial improves a seedling-cultivating method for survival rate, it is characterized in that, comprises the following steps:
A. parent selects
Select physical health, color and luster is normal, movable strong parent, the feeding water earthworm of 3 ~ 5 days;
B. artificial induced spawning
At 24 ~ 28 DEG C of water temperatures, female male parent is carried out to artificial induced spawning, injectable drug is luteinizing hormone releasing hormone A
2, Pan Li ketone, physiological saline, adopt intramuscular injection;
C. fertilization
Natural mating method: the female male parent of having hastened parturition is put into the net cage of laying eggs in 2 ﹕ 1 ratios, then put into all smooth cement pits of five faces, treat its natural spawning fertilization;
D. incubating oosperm
After fertilization, by fertilized egg unsticking, viscidity-removing method is: in fertilized egg, add talcum powder in the ratio of Hua Shi Fen ﹕ fertilized egg=1 ﹕ 3, then after the water that adds 1～2 times stirs, with 80 mesh sieves filtrations, wash away after talcum powder, fertilized egg is put into taper hatching pail and hatch, each taper hatching pail can be hatched 5,000,000～7,000,000 loach seedlings, and hatching water temperature requires at 23 ~ 28 DEG C;
E. loach seedling is raised
After 48 hours, spray scatters in pond, while starting to move about food finding, just can feed loach seedling open-mouthed bait, feeding quantity is calculated by 0.5% of loach seedling gross weight, and feeding method is: open-mouthed bait is placed in plate, put in every square metre of ratio of putting a dish, after two hours, take out plate, from feeding first day, will change water every day, changing water method is: first bleed off in cement pit 1/3rd Chi Shui, then injecting clear water; In the time that loach seedling grows to 2cm, cut off open-mouthed bait, make live body silk earthworm feeding into, still to change water every day, cement pit seedling density will be controlled at 3000～5000 tails/m
2;
F. loach seedling maturation
When after 12 days, loach seedling has grown up to 3cm, and change can be put loach seedling in a suitable place to breed in the large pool, also can start to sell as seed; Large pool cultivation 15～20 days, loach seedling can grow up to 6～8cm, sold until become commodity loach, also can in cement pit, cultivate commodity loach and sell.
2. loach industrial artificial breeding according to claim 1 improves the seedling-cultivating method of survival rate, it is characterized in that, injecting method concrete in described step B is: on operating desk, pad the cotton felt that 0.5 ~ 1cm is thick, separately do the circle of a 15cm diameter, upper seam gauze, in order to cover the loach parent who is placed on cotton felt, adopt the mode of intramuscular injection that oxytocins is expelled in loach body.
3. loach industrial artificial breeding according to claim 1 improves the seedling-cultivating method of survival rate, it is characterized in that, the processing method of described loach seedling open-mouthed bait is: 60% earthworm is worn into slurry and the 10% new fresh milk that squeezes mixes, add the white young material of 30% eel stir alive agglomerating then make little granular.
4. loach industrial artificial breeding according to claim 1 improves the seedling-cultivating method of survival rate, it is characterized in that, the mesh of the described net cage of laying eggs is 9 orders, the thick 1mm of nettle, and the large 2mm in hole, specification is long 2m × wide 1m × high 0.5m.
5. loach industrial artificial breeding according to claim 1 improves the seedling-cultivating method of survival rate, it is characterized in that, the back cut diameter of described taper hatching pail is 1 meter, and 0.2 meter of lower port diameter is high 1 meter.
6. loach industrial artificial according to claim 1 breeding improves the seedling-cultivating method of survival rate, it is characterized in that, the water in described step e is underground water, pH=6.5 ~ 7 of water, and the dissolved oxygen amount of water is 8mg/L at least.
7. loach industrial artificial breeding according to claim 1 improves the seedling-cultivating method of survival rate, it is characterized in that, five described smooth cement pit degree of depth are 70 ~ 90cm.
8. loach industrial artificial breeding according to claim 1 improves the seedling-cultivating method of survival rate, it is characterized in that, described fertilization method can also be crowded smart method: the seminal fluid of manually extruding a male loach, then add 0.2%～0.3% normal saline dilution, the consumption of the physiological saline adding be the ovum that produces of 3 female loach volume 1/3rd, then seminal fluid is poured in the ovum that 3 female loach produce, and stirred with goose feather, it is evenly fertilized.
9. loach industrial artificial breeding according to claim 1 improves the seedling-cultivating method of survival rate, it is characterized in that, described fertilization method can also be got seminal vesicle method for cuing open: get a public loach, cut belly open, take out seminal vesicle and extrude seminal fluid, then add 0.2%～0.3% normal saline dilution, the consumption of the physiological saline adding be the ovum that produces of 10 female loach volume 1/3rd, then seminal fluid is poured in the ovum that 10 female loach produce, and stirred with goose feather, it is evenly fertilized.
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| CN104335937A (en) * | 2014-08-08 | 2015-02-11 | 襄阳远丰农业科技发展有限公司 | Breeding method of Taiwan loaches |
| CN104542398A (en) * | 2015-01-04 | 2015-04-29 | 铜陵县博陵农业生态园 | Spring and autumn two-season propagation method for loaches |
| CN105475197A (en) * | 2015-12-19 | 2016-04-13 | 太和县顺发养殖有限公司 | Loach larva incubation and cultivation method |
| CN105532533A (en) * | 2015-12-29 | 2016-05-04 | 太和县旭升水产养殖有限公司 | Fry cultivating method for loaches |
| CN105557563A (en) * | 2014-10-10 | 2016-05-11 | 龙门县新瑶种养专业合作社 | Taiwan loach high-efficiency industrialized breeding method |
| CN105594636A (en) * | 2015-12-19 | 2016-05-25 | 太和县顺发养殖有限公司 | Efficient artificial culture method for good-quality loach |
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