CN104782549A - Artificial carassius auratus propagation method - Google Patents
Artificial carassius auratus propagation method Download PDFInfo
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- CN104782549A CN104782549A CN201510216649.1A CN201510216649A CN104782549A CN 104782549 A CN104782549 A CN 104782549A CN 201510216649 A CN201510216649 A CN 201510216649A CN 104782549 A CN104782549 A CN 104782549A
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- 238000000034 method Methods 0.000 title claims abstract description 33
- 241000252229 Carassius auratus Species 0.000 title abstract 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 67
- 241000251468 Actinopterygii Species 0.000 claims abstract description 52
- 230000000384 rearing effect Effects 0.000 claims abstract description 11
- 230000032696 parturition Effects 0.000 claims abstract description 7
- 229940088594 vitamin Drugs 0.000 claims abstract description 6
- 229930003231 vitamin Natural products 0.000 claims abstract description 6
- 235000013343 vitamin Nutrition 0.000 claims abstract description 6
- 239000011782 vitamin Substances 0.000 claims abstract description 6
- 150000003722 vitamin derivatives Chemical class 0.000 claims abstract description 6
- 230000012447 hatching Effects 0.000 claims description 37
- 238000002347 injection Methods 0.000 claims description 28
- 239000007924 injection Substances 0.000 claims description 28
- 230000037396 body weight Effects 0.000 claims description 25
- 102000002322 Egg Proteins Human genes 0.000 claims description 24
- 108010000912 Egg Proteins Proteins 0.000 claims description 24
- 210000004681 ovum Anatomy 0.000 claims description 24
- 230000008774 maternal effect Effects 0.000 claims description 19
- 238000004659 sterilization and disinfection Methods 0.000 claims description 19
- 241001300611 Carassius auratus auratus Species 0.000 claims description 17
- 241000252233 Cyprinus carpio Species 0.000 claims description 17
- 238000011049 filling Methods 0.000 claims description 13
- 230000001954 sterilising effect Effects 0.000 claims description 13
- 241000246211 Carassius auratus red var. Species 0.000 claims description 11
- 230000003203 everyday effect Effects 0.000 claims description 11
- 238000007913 intrathecal administration Methods 0.000 claims description 11
- 210000000582 semen Anatomy 0.000 claims description 11
- 239000004568 cement Substances 0.000 claims description 8
- 230000000366 juvenile effect Effects 0.000 claims description 8
- 239000012528 membrane Substances 0.000 claims description 8
- 102000015781 Dietary Proteins Human genes 0.000 claims description 5
- 108010010256 Dietary Proteins Proteins 0.000 claims description 5
- 235000021245 dietary protein Nutrition 0.000 claims description 5
- 230000004720 fertilization Effects 0.000 claims description 5
- 239000004459 forage Substances 0.000 claims description 5
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 claims description 5
- 239000010931 gold Substances 0.000 claims description 5
- 229910052737 gold Inorganic materials 0.000 claims description 5
- 239000007788 liquid Substances 0.000 claims description 5
- 238000002156 mixing Methods 0.000 claims description 5
- 239000004698 Polyethylene Substances 0.000 claims description 4
- -1 polyethylene Polymers 0.000 claims description 4
- 229920000573 polyethylene Polymers 0.000 claims description 4
- 241000167854 Bourreria succulenta Species 0.000 claims description 3
- 235000019693 cherries Nutrition 0.000 claims description 3
- 239000004615 ingredient Substances 0.000 claims description 3
- 239000000463 material Substances 0.000 claims description 3
- 239000001993 wax Substances 0.000 claims description 3
- 239000003814 drug Substances 0.000 abstract description 10
- 238000011534 incubation Methods 0.000 abstract description 9
- 230000000694 effects Effects 0.000 abstract description 7
- 238000013461 design Methods 0.000 abstract description 5
- 230000026109 gonad development Effects 0.000 abstract description 2
- 230000017448 oviposition Effects 0.000 abstract description 2
- 239000003048 aphrodisiac agent Substances 0.000 abstract 2
- 230000002509 aphrodisiac effect Effects 0.000 abstract 2
- 229940079593 drug Drugs 0.000 abstract 2
- 235000013601 eggs Nutrition 0.000 abstract 1
- 230000001902 propagating effect Effects 0.000 abstract 1
- 230000001568 sexual effect Effects 0.000 description 10
- 230000001488 breeding effect Effects 0.000 description 6
- 230000009027 insemination Effects 0.000 description 6
- 238000009395 breeding Methods 0.000 description 5
- 230000001737 promoting effect Effects 0.000 description 5
- ODINCKMPIJJUCX-UHFFFAOYSA-N Calcium oxide Chemical compound [Ca]=O ODINCKMPIJJUCX-UHFFFAOYSA-N 0.000 description 4
- 230000016087 ovulation Effects 0.000 description 3
- ZKQDCIXGCQPQNV-UHFFFAOYSA-N Calcium hypochlorite Chemical compound [Ca+2].Cl[O-].Cl[O-] ZKQDCIXGCQPQNV-UHFFFAOYSA-N 0.000 description 2
- 240000000233 Melia azedarach Species 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 239000007844 bleaching agent Substances 0.000 description 2
- 235000012255 calcium oxide Nutrition 0.000 description 2
- 239000000292 calcium oxide Substances 0.000 description 2
- 239000000919 ceramic Substances 0.000 description 2
- 230000012173 estrus Effects 0.000 description 2
- 238000009396 hybridization Methods 0.000 description 2
- 208000015181 infectious disease Diseases 0.000 description 2
- 230000001988 toxicity Effects 0.000 description 2
- 231100000419 toxicity Toxicity 0.000 description 2
- FFRBMBIXVSCUFS-UHFFFAOYSA-N 2,4-dinitro-1-naphthol Chemical group C1=CC=C2C(O)=C([N+]([O-])=O)C=C([N+]([O-])=O)C2=C1 FFRBMBIXVSCUFS-UHFFFAOYSA-N 0.000 description 1
- 244000025254 Cannabis sativa Species 0.000 description 1
- 241000405070 Percophidae Species 0.000 description 1
- 244000007853 Sarothamnus scoparius Species 0.000 description 1
- 210000001015 abdomen Anatomy 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 230000001276 controlling effect Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000013020 embryo development Effects 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 210000004907 gland Anatomy 0.000 description 1
- 230000002710 gonadal effect Effects 0.000 description 1
- 230000013011 mating Effects 0.000 description 1
- 230000035800 maturation Effects 0.000 description 1
- 210000003205 muscle Anatomy 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 235000018102 proteins Nutrition 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 230000027272 reproductive process Effects 0.000 description 1
- 239000011435 rock Substances 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The invention provides an artificial carassius auratus propagation method. The method is creatively improved in the aspects from the source of parent selection and parent fish rearing to the following aphrodisiac drug preference, incubation method design and the like, a complete technological method for artificially propagating carassius auratus is formed, and the propagation rate, the spawning rate and the incubation rate are remarkably increased. By the adoption of the preferred technical scheme, vitamin is added to fodder before parturition to promote gonad development of parent fish; the preferred temperature of water where the parent fish injected with aphrodisiac drug are located ranges from 21 DEG C to 22 DEG C, so that parent fish rutting and egg laying efficiency is further improved; the preferred incubation temperature ranges from 20 DEG C to 23 DEG C so that fertile eggs can be incubated. By the adoption of the technical scheme, a series of creative design is conducted on the technological conditions, the remarkable technical effect is achieved, the condition control angle and the condition control depth are remarkably improved or optimized compared with those in the prior art, and meanwhile the method is easy to implement, low in cost and remarkable in large-scale application prospect.
Description
Technical field
The present invention relates to technical field of fish culture, be specifically related to gold crucian carp artificial fecundation method.
Background technology
Gold crucian carp is as a kind of Hybrid, have little, carry on the back high, body is thick, body is golden yellow, growth is fast, output is high, the advantages such as premunition is strong, easily raise simultaneously, and protein in dressed fish and muscle, amino acid content are high, carry out spread cultivation at the pond of the provinces and cities such as China Tianjin, Hebei, Shandong, Inner Mongol, reservoir, net cage etc. at present, all show excellent culture efficiency and obvious economic benefit.
Just because of gold crucian carp is a kind of Hybrid, therefore its strain quality, breeding effect directly affect the quality of final finished fish.In the gold crucian carp breeding process of prior art, different researcher is ubiquity difference in Juvenile stage, Reproduction Conditions etc.All in all, the gold crucian carp artificial fecundation method of prior art designs unreasonable in technological parameter, and meanwhile, the condition control category (namely from which aspect implementation condition controlling) in reproductive process is considered perfect not.Be embodied in effect aspect, the reproduction rate of the gold crucian carp artificial fecundation method of prior art, spawning rate, incubation rate are all relatively low.
Summary of the invention
The present invention is intended to the technological deficiency for prior art, provides a kind of gold crucian carp artificial fecundation method, to solve the lower technical problem of gold crucian carp artificial fecundation method reproduction rate existing in prior art.
Another technical problem that the present invention solves is that gold crucian carp artificial fecundation method spawning rate is lower.
The technical problem again that the present invention solves is that gold crucian carp artificial fecundation method incubation rate is lower.
A kind of gold crucian carp artificial fecundation method, comprises the following steps:
1) Juvenile stage: be 3 ~ 5 ages with the age, body weight be the scattered mirror carp of 3.5 ~ 6kg as female parent, be 2 ~ 3 ages with the age, body weight is that the red crucian carp of 0.15 ~ 0.3kg is as male parent;
2) parent fish rearing: be that the pond of 1 ~ 1.5m is as cultivation pond using the degree of depth, before water filling, cultivation pond is carried out disinfection, 8 ~ 10h water filling after sterilization, with the density of every mu of 90 ~ 110 tails throw in step 1) described in female parent, with the density of every mu of 500 ~ 800 tails throw in step 1) described in male parent, ensure ratio 1:(6 ~ 8 between maternal injected volume and male parent injected volume) simultaneously, the forage volume of throwing something and feeding every day is cultivate fish TBW in pond 1 ~ 3%, and dietary protein level is 25 ~ 35% (w/w);
3) artificial propagation: during the first tenday period of a month in mid-April to May, during water temperature more than 18 DEG C, with per kilogram of body weight correspondence 1750 ~ 1850IU human chorionic gonadtropin, 7.5 ~ 8.5 μ g luteinizing principle analogs, the consumption of 1.25 ~ 1.75mg DOM is to described maternal intrathecal human chorionic gonadtropin, luteinizing principle analog, DOM, with the half of the corresponding above-mentioned consumption of per kilogram of body weight to described male parent intrathecal human chorionic gonadtropin, luteinizing principle analog, DOM, after injection during 8 ~ 10h, with the seminal fluid of 1 maternal ovum correspondence 6 ~ 10 male parent for ratio ovum artificial collection respectively, semen collection liquid, then add water mixing in a reservoir, fertilization,
4) step 3 is got) fertilized egg that obtains, in 16 ~ 23 DEG C of water bodys, utilize fish nest to hatch or circular hatching channal Lotic hatching, membrane, obtain gold carp fry.
Preferably, step 2) in parent fish rearing, in feed, add vitamin ingredients just before giving birth, wash by water every day 2 ~ 3h simultaneously.
Preferably, step 3) male parent after injection and maternal separately grain bin be placed in two cement pits.Preferred further on this basis, the specification of described cement pit is: area 20 ~ 30m
2, depth of water 50-70cm.
Preferably, step 3) injection after parent population residing for water temperature be 21 ~ 22 DEG C.
Preferably, step 3) injection to female parent or the injection to male parent make two bites at a cherry, and the 1/3rd, second pin injection surplus of full dosage injected by the first pin.
Preferably, step 4) hatching of described fish nest comprises the following steps: with polyethylene mesh sheet for material builds fish nest, by step 3 after sterilization) fertilized egg that obtains splashes on fish nest, after 30 ~ 40min, the fish nest with fertilized egg sunk to below the water surface 10 ~ 15cm, hatching.
Preferably, step 4) described in circular hatching channal Lotic hatching comprise the following steps: by step 3) fertilized egg that obtains splashes and to disperse in inwall waxes the spawning pond that processed, utilize egg-collecting box to collect fertilized egg after 2.5 ~ 3.5h, then fertilized egg is placed in circular hatching channal with the density of every cubic metre of water body correspondence 60 ~ 800,000 hatches.
Preferably, step 4) described in water temperature be 20 ~ 23 DEG C.
Preferably, step 3) described in consumption be the corresponding 1800IU human chorionic gonadtropin of per kilogram of body weight, 8 μ g luteinizing principle analogs, 1.5mg DOM.
In above technical scheme, from Juvenile stage, the parent fish rearing method in source, to follow-up promote the sexual maturity medicine preferably, hatching m design etc., all make the improvement of novelty, define the process of the complete set of gold crucian carp artificial propagation, thus significantly improve reproduction rate, spawning rate and incubation rate.
In optimal technical scheme, add vitamin just before giving birth and adhere to that bath every day is conducive to promoting the growth of parent population sexual gland.The promote the sexual maturity female parent after medicine and male parent of injection is placed in the Best Times that little Chi contributes to holding artificial insemination respectively.Water temperature residing for the parent population after medicine of injection being promoted the sexual maturity is preferably 21 ~ 22 DEG C, be the parent finding under this temperature condition after this medicine of promoting the sexual maturity of this injection oestrus, ovulate most effective.And to be designed to inject at twice to the injection of medicine of promoting the sexual maturity be also because injection pattern in this is conducive to medicine generating effect.In addition, two kinds of incubation modes that the technical program provides all can play good hatching effect, and user can according to self actual unrestricted choice.It is find that hatching efficiency is higher under this temperature condition that incubation temperature is preferably 20 ~ 23 DEG C.
Technical solution of the present invention is by a series of innovative designs to process conditions; achieve outstanding technique effect, the angle that condition controls and the degree of depth comparatively prior art have significantly improvement or optimize, and the present invention is simultaneously easy to realize; cost is lower, has outstanding scale application prospect.
Embodiment
Below will be described in detail the specific embodiment of the present invention.In order to avoid too much unnecessary details, in the examples below to belonging to known structure or function will not be described in detail.Apart from outside definition, technology used in following examples and scientific terminology have the identical meanings generally understood with those skilled in the art of the invention.
Embodiment 1
1, Juvenile stage
(1) maternal
Scattered mirror carp, requires to select bodily form spindle-type, body surface without wound, the age be 3 ~ 5 ages, individual weight 3.5 ~ 6.0kg individuality as parent population.
(2) male parent
Red crucian carp, require to select the bodily form moderate, body colour is pure red, and without assorted flower without wound, physique is healthy and strong, the age be 2 ~ 3 ages, individual weight 150 ~ 300g individuality as parent population.
(3) parent population property ratio
Female parent and male parent parent population ratio are in 1:6 ~ 8.Parent also will once select and remain before breeding, the individuality that sexual maturity of mainly selecting and remain is good, to ensure high reproduction rate, spawning rate and incubation rate.
2. parent fish rearing
(1) pond condition is cultivated
Parent fish pond requires at the bottom of pond smooth, and mud is more shallow, and it is convenient to enter draining, the depth of water at 1 ~ 1.5m, area about 2 ~ 3 mu.
(2) fishpond sterilization
Under parent population before pond, strict sterilization should be carried out once in pond.General sterilization quicklime or bleaching powder, its consumption is respectively: every 667m
2use quicklime 75kg, be made into limewash full Chi Fa and spill, 10 ~ 15d lack of toxicity, or every 667m
2use bleaching powder 20kg, after being dissolved in water in a reservoir, full pool spilling head, 7 ~ 10d lack of toxicity.
(3) water filling in fishpond
Just can water filling after 8 ~ 10h through the pond of sterilization, water filling water source requires to meet national standard.When with water source, pond, must strict filtration be carried out and disinfect.
(4) breeding density and feeding and management
Scattered mirror carp: body weight is 3.5 ~ 6.0kg, can put 100 tails in a suitable place to breed for every mu, and can overlap Lian, Bighead fish of foster some.
Red crucian carp: body weight is 150 ~ 300g, can put 500 ~ 800 tails in a suitable place to breed for every mu, and can overlap Lian, Bighead fish of foster some.
Under parent population behind pond, must strengthen feeding and management, bait is is generally thrown something and fed Articial bait making, and protein content is about 30%.Day feeding volume accounts for 1% ~ 3% of fish TBW, throws something and feeds every day 2 ~ 3 times.Spring before just before giving birth should add a certain amount of vitamin in cultivating in feed.Adhere to the 2 ~ 3h that washes by water every day, to promote the gonad development of parent population simultaneously.
3. artificial propagation
(1) mating season
The breeding of carp crucian generally in the first tenday period of a month in mid-April to May, when water temperature stability is more than 18 DEG C, breeding of can promoting the sexual maturity.
(2) hasten parturition
In order to improve female scattered mirror carp egg laying amount and obtain fry in enormous quantities, artificial propagation must be carried out.Promote the sexual maturity medicine human chorionic gonadtropin (HCG) with luteinizing principle analog (LRH-A2) and DOM (DOM), dosage is per kilogram of body weight HCG1800IU+LRH-A28 μ g+DOM1.5mg, carries out twice intrathecal.First pin injects 1/3rd of full dosage, the second pin injection surplus.The red crucian carp injected dose of milter is the half of raun.Effect time, gonadal maturation is good, water temperature 21-22 DEG C, and after injection, 8 ~ 10h just can oestrus ovulation.For holding test-tube Best Times, injecting the raun of medicine of promoting the sexual maturity and milter and should store up respectively and be placed on (cement pit area is generally 20 ~ 30m2, and the depth of water is 50-70cm) in the net cage of Small cement pond and check raun ovulation situation at any time.Milter is general better ripe.
(3) artificial insemination
According to Effect time, then check its ovulation situation, when light pressure raun belly has ovum grain to flow out, carry out ovum artificial collection immediately.The scattered mirror carp of general 4.0kg can adopt ovum 60-70 ten thousand, can join the male parent red crucian carp 6-10 tail of more than 150g.If the red crucian carp of male parent is individual large, sperm is abundant, and sperm quality is good, can reduce male parent red crucian carp quantity 2-3 tail.During ovum artificial collection, with clean ceramic whiteware basin splendid attire, when adopting ovum and reaching some (covering the basinful end), clamp-on 5-6 immediately and drip seminal fluid, rock ceramic whiteware basin gently, smart ovum is fully mixed, then add water (needing certain Height Impact essence ovum), gently stir smart ovum with have gentle hands simultaneously, make it fully be fertilized, and disperse granulating.Artificial insemination should in the cool or indoor carry out, avoid light direct projection.When carrying out ovum artificial collection and insemination, must dry the water on fish body, each ovum artificial collection and insemination operating time must not more than 3min.If parent population quantity is many, then continuously to carries out continuous productive process, carry out artificial insemination, while fertilized egg unsticking or transfer on fish nest is hatched.
(4) hatch
1. hatching m
Hybridization ovum is viscid egg, and hatching m has two kinds.
Fish nest is hatched.Artificial fish nest grass green polyethylene mesh sheet is made.First fish nest is carried out sterilization processing during use, after fish nest is layered on preprepared, the depth of water in the cement pit of more than 50cm, then by fertilized egg with light the splashing on fish nest of have gentle hands, allow it disperse equably and adhere on fish nest.After about 30-40min, it is cursory to be bound by the fish nest with fertilized egg, then links up one by one, puts into hatchery and hatches.Fish nest will sink to below water surface 10-15cm.
Circular hatching channal Lotic hatching.Fertilized egg is splashed in " chinese carp " spawning pond." chinese carp " spawning pond will " be waxed " in advance, makes its smooth surface.After about 3h, sweep ovum lightly with broom and make it enter in egg-collecting box with current, then rinse fertilized egg with clear water, make it disperse granulating, put into circuit, carry out Lotic hatching.Put into the fertilized egg amount of circular hatching channal, every cube of water body is about 60 ~ 800,000.The water inlet impulse force of the Duckbill type water injection pipe arranged by circuit bottom, makes fish-egg evenly roll and hatch in circular hatching channal.
2. hatching management
Prevent the infection of water mo(u)ld.When finding that there is a small amount of ovum infection water mold, with herbal mediciment, the fertilized egg in fish nest or circuit water is carried out disinfection.
Clean screen window at any time, prevent excessive.Observe the time of fish-egg rupture of membranes, note regulating current.
The hatching water temperature of hybridization ovum is 16 DEG C-23 DEG C, and 16 DEG C of-18 DEG C of embryonic developments are comparatively slow, and grow best when 20 DEG C-23 DEG C, fertilized egg is through the complete membrane of 70 ~ 80h, and waist point appears in membrane after three days, can wander up and down number to sell or lower pond is cultivated.
Embodiment 2
A kind of gold crucian carp artificial fecundation method, is characterized in that comprising the following steps:
1) Juvenile stage: be 3 ages with the age, body weight be the scattered mirror carp of 3.5kg as female parent, be 2 ages with the age, body weight is that the red crucian carp of 0.15kg is as male parent;
2) parent fish rearing: be that the pond of 1m is as cultivation pond with the degree of depth, before water filling, cultivation pond is carried out disinfection, 8h water filling after sterilization, with the density of every mu of 90 tails throw in step 1) described in female parent, with the density of every mu of 500 tails throw in step 1) described in male parent, ensure the ratio 1:6 between maternal injected volume and male parent injected volume, the forage volume of throwing something and feeding every day is cultivate fish TBW in pond 1%, and dietary protein level is 25% (w/w) simultaneously;
3) artificial propagation: during the first tenday period of a month in mid-April to May, during water temperature more than 18 DEG C, with the corresponding 1750IU human chorionic gonadtropin of per kilogram of body weight, 7.5 μ g luteinizing principle analogs, the consumption of 1.25mg DOM is to described maternal intrathecal human chorionic gonadtropin, luteinizing principle analog, DOM, with the half of the corresponding above-mentioned consumption of per kilogram of body weight to described male parent intrathecal human chorionic gonadtropin, luteinizing principle analog, DOM, after injection during 8h, with the seminal fluid of corresponding 6 male parents of 1 maternal ovum for ratio ovum artificial collection respectively, semen collection liquid, then add water mixing in a reservoir, fertilization,
4) step 3 is got) fertilized egg that obtains, in 16 DEG C of water bodys, utilize fish nest to hatch or circular hatching channal Lotic hatching, membrane, obtain gold carp fry.
Embodiment 3
A kind of gold crucian carp artificial fecundation method, is characterized in that comprising the following steps:
1) Juvenile stage: be 5 ages with the age, body weight be the scattered mirror carp of 6kg as female parent, be 3 ages with the age, body weight is that the red crucian carp of 0.3kg is as male parent;
2) parent fish rearing: be that the pond of 1.5m is as cultivation pond with the degree of depth, before water filling, cultivation pond is carried out disinfection, 10h water filling after sterilization, with the density of every mu of 110 tails throw in step 1) described in female parent, with the density of every mu of 800 tails throw in step 1) described in male parent, ensure the ratio 1:8 between maternal injected volume and male parent injected volume, the forage volume of throwing something and feeding every day is cultivate fish TBW in pond 3%, and dietary protein level is 35% (w/w) simultaneously;
3) artificial propagation: during the first tenday period of a month in mid-April to May, during water temperature more than 18 DEG C, with the corresponding 1850IU human chorionic gonadtropin of per kilogram of body weight, 8.5 μ g luteinizing principle analogs, the consumption of 1.75mg DOM is to described maternal intrathecal human chorionic gonadtropin, luteinizing principle analog, DOM, with the half of the corresponding above-mentioned consumption of per kilogram of body weight to described male parent intrathecal human chorionic gonadtropin, luteinizing principle analog, DOM, after injection during 10h, with the seminal fluid of corresponding 10 male parents of 1 maternal ovum for ratio ovum artificial collection respectively, semen collection liquid, then add water mixing in a reservoir, fertilization,
4) step 3 is got) fertilized egg that obtains, in 23 DEG C of water bodys, utilize fish nest to hatch or circular hatching channal Lotic hatching, membrane, obtain gold carp fry.
Embodiment 4
A kind of gold crucian carp artificial fecundation method, is characterized in that comprising the following steps:
1) Juvenile stage: be 4 ages with the age, body weight be the scattered mirror carp of 5kg as female parent, be 2.5 ages with the age, body weight is that the red crucian carp of 0.2kg is as male parent;
2) parent fish rearing: be that the pond of 1.2m is as cultivation pond with the degree of depth, before water filling, cultivation pond is carried out disinfection, 9h water filling after sterilization, with the density of every mu of 100 tails throw in step 1) described in female parent, with the density of every mu of 700 tails throw in step 1) described in male parent, ensure the ratio 1:7 between maternal injected volume and male parent injected volume, the forage volume of throwing something and feeding every day is cultivate fish TBW in pond 2%, and dietary protein level is 30% (w/w) simultaneously;
3) artificial propagation: during the first tenday period of a month in mid-April to May, during water temperature more than 18 DEG C, with the corresponding 1800IU human chorionic gonadtropin of per kilogram of body weight, 8 μ g luteinizing principle analogs, the consumption of 1.5mg DOM is to described maternal intrathecal human chorionic gonadtropin, luteinizing principle analog, DOM, with the half of the corresponding above-mentioned consumption of per kilogram of body weight to described male parent intrathecal human chorionic gonadtropin, luteinizing principle analog, DOM, after injection during 9h, with the seminal fluid of corresponding 8 male parents of 1 maternal ovum for ratio ovum artificial collection respectively, semen collection liquid, then add water mixing in a reservoir, fertilization,
4) step 3 is got) fertilized egg that obtains, in 19 DEG C of water bodys, utilize fish nest to hatch or circular hatching channal Lotic hatching, membrane, obtain gold carp fry.
On the basis of above technical scheme, meet the following conditions:
Step 2) in parent fish rearing, just before giving birth, in feed, add vitamin ingredients, wash by water every day 2.5h simultaneously.
Step 3) male parent after injection and maternal separately grain bin be placed in two cement pits, this sump area 20 ~ 30m
2, depth of water 50-70cm.
Step 3) injection after parent population residing for water temperature be 21 ~ 22 DEG C.
Step 3) injection to female parent or the injection to male parent make two bites at a cherry, and the 1/3rd, second pin injection surplus of full dosage injected by the first pin.
Step 4) hatching of described fish nest comprises the following steps: with polyethylene mesh sheet for material builds fish nest, by step 3 after sterilization) fertilized egg that obtains splashes on fish nest, after 35min, the fish nest with fertilized egg sunk to below water surface 12cm, hatching.
Step 4) described in circular hatching channal Lotic hatching comprise the following steps: by step 3) fertilized egg that obtains splashes and to disperse in inwall waxes the spawning pond that processed, utilize egg-collecting box to collect fertilized egg after 3h, then fertilized egg is placed in circular hatching channal with the density of corresponding 700,000 of every cubic metre of water body hatches.
Above embodiments of the invention have been described in detail, but described content is only preferred embodiment of the present invention, not in order to limit the present invention.All make in application range of the present invention any amendment, equivalent to replace and improvement etc., all should be included within protection scope of the present invention.
Claims (10)
1. a gold crucian carp artificial fecundation method, is characterized in that comprising the following steps:
1) Juvenile stage: be 3 ~ 5 ages with the age, body weight be the scattered mirror carp of 3.5 ~ 6kg as female parent, be 2 ~ 3 ages with the age, body weight is that the red crucian carp of 0.15 ~ 0.3kg is as male parent;
2) parent fish rearing: be that the pond of 1 ~ 1.5m is as cultivation pond using the degree of depth, before water filling, cultivation pond is carried out disinfection, 8 ~ 10h water filling after sterilization, with the density of every mu of 90 ~ 110 tails throw in step 1) described in female parent, with the density of every mu of 500 ~ 800 tails throw in step 1) described in male parent, ensure ratio 1:(6 ~ 8 between maternal injected volume and male parent injected volume) simultaneously, the forage volume of throwing something and feeding every day is cultivate fish TBW in pond 1 ~ 3%, and dietary protein level is 25 ~ 35% (w/w);
3) artificial propagation: during the first tenday period of a month in mid-April to May, during water temperature more than 18 DEG C, with per kilogram of body weight correspondence 1750 ~ 1850IU human chorionic gonadtropin, 7.5 ~ 8.5 μ g luteinizing principle analogs, the consumption of 1.25 ~ 1.75mg DOM is to described maternal intrathecal human chorionic gonadtropin, luteinizing principle analog, DOM, with the half of the corresponding above-mentioned consumption of per kilogram of body weight to described male parent intrathecal human chorionic gonadtropin, luteinizing principle analog, DOM, after injection during 8 ~ 10h, with the seminal fluid of 1 maternal ovum correspondence 6 ~ 10 male parent for ratio ovum artificial collection respectively, semen collection liquid, then add water mixing in a reservoir, fertilization,
4) step 3 is got) fertilized egg that obtains, in 16 ~ 23 DEG C of water bodys, utilize fish nest to hatch or circular hatching channal Lotic hatching, membrane, obtain gold carp fry.
2. method according to claim 1, is characterized in that step 2) in parent fish rearing, just before giving birth, in feed, add vitamin ingredients, wash by water every day 2 ~ 3h simultaneously.
3. method according to claim 1, is characterized in that step 3) male parent after injection and maternal separately grain bin be placed in two cement pits.
4. method according to claim 3, is characterized in that the specification of described cement pit is: area 20 ~ 30m
2, depth of water 50-70cm.
5. the method according to 1,3,4 any one, is characterized in that step 3) injection after parent population residing for water temperature be 21 ~ 22 DEG C.
6. method according to claim 1, is characterized in that step 3) injection to female parent or the injection to male parent make two bites at a cherry, and the 1/3rd, second pin injection surplus of full dosage injected by the first pin.
7. method according to claim 1, it is characterized in that step 4) hatching of described fish nest comprises the following steps: with polyethylene mesh sheet for material builds fish nest, by step 3 after sterilization) fertilized egg that obtains splashes on fish nest, after 30 ~ 40min, the fish nest with fertilized egg is sunk to below the water surface 10 ~ 15cm, hatching.
8. method according to claim 1, it is characterized in that step 4) described in circular hatching channal Lotic hatching comprise the following steps: by step 3) fertilized egg that obtains splashes and to disperse in inwall waxes the spawning pond that processed, utilize egg-collecting box to collect fertilized egg after 2.5 ~ 3.5h, then fertilized egg is placed in circular hatching channal with the density of every cubic metre of water body correspondence 60 ~ 800,000 hatches.
9. method according to claim 1, is characterized in that step 4) described in water temperature be 20 ~ 23 DEG C.
10. method according to claim 1, is characterized in that step 3) described in consumption be the corresponding 1800IU human chorionic gonadtropin of per kilogram of body weight, 8 μ g luteinizing principle analogs, 1.5mg DOM.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105494182A (en) * | 2015-12-03 | 2016-04-20 | 天津市金水族水产养殖有限公司 | Breeding method for goldfish |
| CN105746399A (en) * | 2016-03-07 | 2016-07-13 | 五河县金满塘生态种养殖家庭农场 | Crucian breeding quality improvement method |
| CN106172119A (en) * | 2016-07-15 | 2016-12-07 | 天津现代晨辉科技集团有限公司 | Grass goldfish breeding and the method for selection-breeding |
| CN106172118A (en) * | 2016-07-14 | 2016-12-07 | 天津农学院 | A kind of river fish Seedling mating system |
| CN111296330A (en) * | 2020-03-04 | 2020-06-19 | 浙江省海洋水产研究所 | Device and method for breeding parent fish of bream with snout and obtaining fertilized eggs |
| CN112189598A (en) * | 2020-09-30 | 2021-01-08 | 黔东南苗族侗族自治州农业科学院 | Breeding method of golden spot crucian carps |
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2015
- 2015-04-30 CN CN201510216649.1A patent/CN104782549A/en active Pending
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105494182A (en) * | 2015-12-03 | 2016-04-20 | 天津市金水族水产养殖有限公司 | Breeding method for goldfish |
| CN105746399A (en) * | 2016-03-07 | 2016-07-13 | 五河县金满塘生态种养殖家庭农场 | Crucian breeding quality improvement method |
| CN106172118A (en) * | 2016-07-14 | 2016-12-07 | 天津农学院 | A kind of river fish Seedling mating system |
| CN106172119A (en) * | 2016-07-15 | 2016-12-07 | 天津现代晨辉科技集团有限公司 | Grass goldfish breeding and the method for selection-breeding |
| CN111296330A (en) * | 2020-03-04 | 2020-06-19 | 浙江省海洋水产研究所 | Device and method for breeding parent fish of bream with snout and obtaining fertilized eggs |
| CN112189598A (en) * | 2020-09-30 | 2021-01-08 | 黔东南苗族侗族自治州农业科学院 | Breeding method of golden spot crucian carps |
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