CN104642212A - Artificial breeding method for onychostoma simus - Google Patents

Artificial breeding method for onychostoma simus Download PDF

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Publication number
CN104642212A
CN104642212A CN201310578354.XA CN201310578354A CN104642212A CN 104642212 A CN104642212 A CN 104642212A CN 201310578354 A CN201310578354 A CN 201310578354A CN 104642212 A CN104642212 A CN 104642212A
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pond
fry
water
hatching
fish
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蔡焰值
蔡煜
雷晓中
汪亮
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HUBEI AQUATIC SCIENCTIFIC RESEARCH INST
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K61/00Culture of aquatic animals
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/02Breeding vertebrates
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Zoology (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Marine Sciences & Fisheries (AREA)
  • Animal Behavior & Ethology (AREA)
  • Farming Of Fish And Shellfish (AREA)

Abstract

The present invention relates to an artificial breeding method for onychostoma simus. The method is characterized in comprising steps that: selecting and mating eugamic male and female onychostoma simus, conducting artificial spawning induction, conducting natural insemination with a parental female and male ratio of 1:1; suspending the fish nests with fertilized ovum in a hatching barrel, hatching the fertilized ovum in lotic water until fish fry breaks through membrane, putting the fish fry bred from onychostoma simus consistent with economic trait requirement into a raising pond for temporal raising, and till the fish fry is raised to a whole body length over 1.5 cm according to the above requirements, throwing the fish fry into ponds for nurture of fish fingerlings. According to the method, the survival rate of hatched onychostoma simus is largely improved, the yield of fish fry is largely improved, and the economic efficiency of enterprises is increased.

Description

Onychostoma simus artificial breeding method
Technical field
The present invention relates to a kind of onychostoma simus artificial breeding method.
Background technology
Onychostoma simus belongs to eurythermic fish, and adaptive temperature scope is 0-36 DEG C, and the suitableeest long-living temperature 15-28 DEG C that ingests, starts to ingest when water temperature is more than 10 DEG C, and water temperature 30-30.5 DEG C of appetite weakens, and substantially stops eating when water temperature is more than 34 DEG C.Still can normal life when dissolved oxygen is 3.5 ㎎/L, the best growth dissolved oxygen amount of ingesting is 4.0 ㎎/more than L, and when dissolved oxygen in water body is raised the nose above water to breathe lower than starting during 1.0-1.5 ㎎/L, 0.9 ㎎/L is dead.The pH value range grown of normally ingesting is 6.8-8.5, and organic matter oxygen demand is less than 8.5mg/L, and total nitrogen is no more than 0.48mg/L, and nitrite is at below 0.018mg/L.
Summary of the invention
Technical problem to be solved by this invention is: in order to solve the problem mentioned in above-mentioned background technology, the invention provides the onychostoma simus artificial breeding method that a kind of survival rate of seedling is higher.
For solving the problems of the technologies described above, the invention provides onychostoma simus artificial breeding method, its difference is, first selects sexually matured onychostoma simus milter and raun combo, adopt that artificial induced spawning is artificial, natural insemination time, female, male parent population ratio is 1: 1; The spawning season of onychostoma simus is at the bottom of mid-April to May, and water temperature range of laying eggs is 18 DEG C ~ 26 DEG C, and suitable water temperature is 20 DEG C ~ 24 DEG C, uses oxytocic drug to hasten parturition, then carries out natural insemination or artificial insemination; Incubating oosperm, to fry membrane, is then put into and is cultivated pond by meeting fry that parent population breeding that onychostoma simus biology economic characters require obtains and support temporarily, cultivate into the fry that total length is 1.5 ㎝ ~ 2.0 ㎝, then proceed to pond and carry out seed rearing until fingerling goes out pond.
In above scheme, the selection of described milter: kissing the upper jaw mating season has and coarsely chase after the rough hand of raw meat, and belly lateral deviation is gently pressed with seminal fluid and flows out external, gonapophysis end blush; The selection of raun: mating season belly expand, soft, ovary profile is obvious, gonopore relaxes, and have flowing shape after ovary tenesmus, anus is red and swollen, and dig ovum inspection, ovum grain is full, and size is neat, glossy, in buff; Most core off normal is checked by transparent liquid.
In above scheme, described oxytocic drug comprises carp brain hypophysis (PG), pimozide DOM (DOM), fish human chorionic gonadtropin (HCG), fish luteotropin releasing hormone d-ala analog (LRH-A), alepidote hormone and fish efficient induced spawning mixture, oxytocic drug injection system adopts pectoral fin base portion or muscle of back injection, raun oxytocic drug accumulated dose is injected at twice, injected dose is 1/8 ~ 1/10 of accumulated dose for the first time, carry out second time injection after the 10h ~ 16h of interval, injected dose is whole surplus; Milter disposable injection when the injection of raun second time, its drug dose is 1/3 ~ 1/2 of raun accumulated dose.
In above scheme, the concrete grammar of natural insemination is as follows: spawning pond cleans up, a brown sheet laying nest is set bottom pond, press solidly with gravel, add water to 0. 6m ~ 0.8m dark, after parent population second time injection ocyodinic, press 1:1 combo, put into spawning pond, support temporarily with miniflow water, produced after ovum until parent population and pulled fish nest out, be suspended on Lotic hatching in hatching pail; The hatching density being suspended on Lotic hatching in hatching pail is 2.5 × 104, each hatching pail water body; Environment of hatching should meet the following conditions: water quality should meet the regulation of GB 11607, wherein the dissolved oxygen amount of water body should at 6 ㎎/more than L, and pH value is 6.8 ~ 8.2, keep water quality pure and fresh, Spawning water specification is that 24 Kong/㎝ polyamide fibres or terylene mesh filter, and is strictly on guard against that harmful organisms enters; Hatching water temperature range is 20 DEG C ~ 26 DEG C, and suitable water temperature is 22 DEG C ~ 24 DEG C.
In above scheme, by the regulation unsticking of SC/T 1013 after natural insemination, the fertilized egg after unsticking puts into hatching pail Lotic hatching until incubating oosperm is to fry membrane, and the hatching density of unsticking ovum in hatching pail is 30 × 104; Incubating oosperm is as follows to the required time of fry membrane:
Water temperature and fry membrane time relationship
In above scheme, described cultivation pond adopts flowing water cement pit; Its shape is rectangle, and area is 3 ㎡ ~ 5 ㎡, and the depth of water is 50 ㎝ ~ 60 ㎝; The screen cloth screen window of discharge outlet aperture 6mm prevents fry from fleeing, and flowing water cement pit lucifuge object is covered lucifuge condition; Put Miao Qianying by the clear pond sterilization of the regulation of SC/T 1008; Cultivate the regulation that pond water quality should meet GB 11607, water quality is pure and fresh, and wherein water body dissolved oxygen amount should be 6.8 ~ 8.5 at 6 ㎎/more than L, pH, and transparency is 40 ㎝ ~ 45 ㎝, and suitable water temperature is 20 DEG C ~ 28 DEG C, and fry puts into water temperature difference when cultivating pond and is no more than ± and 2 DEG C; Every cubic metre of water body puts 2 × 104 tail ~ 3 × 104 tails, treat that fry can freely move about and fish body is covered with melanin, after yolk sac major part absorbs, start first to open food with yolk water liquid, to throw something and feed again living body biological bait wheel animalcule, cultivate into the fry that total length is 1.5 ㎝ ~ 2.0 ㎝; Flowing water pond should keep uninterrupted flowing water, is changed once and blowdown by the water body in flowing water pond about 80% every 2d, or with aerator to water oxygenation; Directly to throw something and feed living body biological bait wheel animalcule in cultivation pond when fry starts to ingest.
In above scheme, will support that to cultivate into total length be that the fry of 1.5 ㎝ ~ 2.0 ㎝ is dropped into pond and carries out nurture of fish fingerlings temporarily cultivating pond, fry can ingest zooplankton; Fry neat specification, body colour are consistent, travelling active, anosis, nothing wound, nothing deformity; Outside the regulation of pond by SC/T 1008, pond area is 1 500 ㎡ ~ 2 000 ㎡, and pond shape is neat, and smooth at the bottom of pond, silt depth 6 ㎝ ~ 10 ㎝, has abundant water resources, pollution-free source, and irrigation and drainage are convenient; The sterilization of seed rearing pond should meet the regulation of SC/T 1008 and NY 5071 respectively with cultivating quality of water, after cultivating a large amount of zooplankton and transparency and being 45 ㎝, throw in fry again; The breeding density of fry with go out pond specification and see the following form:
Fry breeding density with go out pond specification
Technique scheme of the present invention has the following advantages compared with existing general approach: the survival rate that onychostoma simus is hatched improves greatly, drastically increases the output of fry, adds the economic benefit of enterprise.
Embodiment
Embodiment of the present invention onychostoma simus artificial breeding method, is characterized in that, selects sexually matured onychostoma simus milter and raun combo, adopt that artificial induced spawning is artificial, natural insemination time, female, male parent population ratio is 1: 1, milter: kissing the upper jaw mating season has and coarsely chase after the rough hand of raw meat, and belly lateral deviation is gently pressed with seminal fluid and flows out external, gonapophysis end blush, raun: mating season belly expand, soft, ovary profile is obvious, gonopore relaxes, and have flowing shape after ovary tenesmus, anus is red and swollen, and dig ovum inspection, ovum grain is full, and size is neat, glossy, in buff, most core off normal is checked by transparent liquid, the spawning season of onychostoma simus is at the bottom of mid-April to May, water temperature range of laying eggs is 18 DEG C ~ 26 DEG C, suitable water temperature is 20 DEG C ~ 24 DEG C, oxytocic drug comprises carp brain hypophysis (PG), pimozide DOM (DOM), fish is with human chorionic gonadtropin (HCG), fish is with luteotropin releasing hormone d-ala analog (LRH-A), alepidote hormone and fish efficient induced spawning mixture, oxytocic drug injection system adopts pectoral fin base portion or muscle of back injection, raun oxytocic drug accumulated dose is injected at twice, injected dose is 1/8 ~ 1/10 of accumulated dose for the first time, second time injection is carried out after the 10h ~ 16h of interval, injected dose is whole surplus, milter disposable injection when the injection of raun second time, its drug dose is 1/3 ~ 1/2 of raun accumulated dose, then natural insemination or artificial insemination is carried out, the concrete grammar of natural insemination is as follows: spawning pond cleans up, a brown sheet laying nest is set bottom pond, press solidly with gravel, add water to 0. 6m ~ 0.8m dark, press 1:1 combo after parent population second time injection ocyodinic, put into spawning pond, support temporarily with miniflow water, produce after ovum until parent population and pulled fish nest out, be suspended on Lotic hatching in hatching pail, environment of hatching should meet the following conditions: water quality should meet the regulation of GB 11607, wherein the dissolved oxygen amount of water body should at 6 ㎎/more than L, and pH value is 6.8 ~ 8.2, keep water quality pure and fresh, Spawning water specification is that 24 Kong/㎝ polyamide fibres or terylene mesh filter, and is strictly on guard against that harmful organisms enters, hatching water temperature range is 20 DEG C ~ 26 DEG C, and suitable water temperature is 22 DEG C ~ 24 DEG C, hatching pattern: will the fish nest of fertilized egg be stained with, and be suspended on Lotic hatching in hatching pail, the hatching density being suspended on Lotic hatching in hatching pail is 2.5 × 104, each hatching pail water body, by the regulation unsticking of SC/T 1013, the fertilized egg after unsticking puts into hatching pail Lotic hatching until incubating oosperm is to fry membrane, and the hatching density of unsticking ovum in hatching pail is 30 × 104, incubating oosperm is as follows to the required time of fry membrane:
Table 2 water temperature and fry membrane time relationship
The fry meeting the parent population breeding acquisition that onychostoma simus biology economic characters require is put into cultivation pond support temporarily, cultivate pond and adopt flowing water cement pit; Its shape is rectangle, and area is 3 ㎡ ~ 5 ㎡, and the depth of water is 50 ㎝ ~ 60 ㎝; The screen cloth screen window of discharge outlet aperture 6mm prevents fry from fleeing, and flowing water cement lucifuge object is covered lucifuge condition.Put Miao Qianying by the clear pond sterilization of the regulation of SC/T 1008; Cultivate the regulation that pond water quality should meet GB 11607, water quality is pure and fresh, and wherein water body dissolved oxygen amount should be 6.8 ~ 8.5 at 6 ㎎/more than L, pH, and transparency is 40 ㎝ ~ 45 ㎝.Suitable water temperature is 20 DEG C ~ 28 DEG C, fry put into cultivate pond time water temperature difference be no more than ± 2 DEG C; Every cubic metre of water body puts 2 × 104 tail ~ 3 × 104 tails, treat that fry can freely move about and fish body is covered with melanin, after yolk sac major part absorbs, start first to open food with yolk water liquid, to throw something and feed again living body biological bait wheel animalcule, cultivate into the fry that total length is 1.5 ㎝ ~ 2.0 ㎝.Proceed to pond again and carry out seed rearing; Flowing water pond should keep uninterrupted flowing water, every 2d, the water body in flowing water pond is changed once (about 80%) and blowdown, or with aerator to water oxygenation; Directly to throw something and feed living body biological bait wheel animalcule in cultivation pond when fry starts to ingest; Cultivating into total length by above-mentioned requirements is that the fry of 1.5 more than ㎝ is dropped into pond and carries out nurture of fish fingerlings, and fry can ingest zooplankton; Fry neat specification, body colour are consistent, travelling active, anosis, nothing wound, nothing deformity; Outside the regulation of pond by SC/T 1008, pond area is 1 500 ㎡ ~ 2 000 ㎡, and pond shape is neat, and smooth at the bottom of pond, silt depth 6 ㎝ ~ 10 ㎝, has abundant water resources, pollution-free source, and irrigation and drainage are convenient; The sterilization of seed rearing pond should meet the regulation of SC/T 1008 and NY 5071 respectively with cultivating quality of water, after cultivating a large amount of zooplankton and transparency and being 45 ㎝, throw in fry again; The breeding density of fry with go out pond specification and see the following form:
Fry breeding density with go out pond specification
spawning rate according to above-mentioned technical requirement parent population can reach more than 92%, and fertilization rate reached is to 85%, and emergence rate reaches more than 95%, and fry is trained motility rate up to more than 88%.
With above-mentioned according to desirable embodiment of the present invention for enlightenment, by above-mentioned description, relevant staff in the scope not departing from this invention technological thought, can carry out various change and amendment completely.The technical scope of this invention is not limited to the content on specification, must determine its technical scope according to right.

Claims (7)

1. onychostoma simus artificial breeding method, is characterized in that, first selects sexually matured onychostoma simus milter and raun combo, adopt that artificial induced spawning is artificial, natural insemination time, female, male parent population ratio is 1: 1; The spawning season of onychostoma simus is at the bottom of mid-April to May, and water temperature range of laying eggs is 18 DEG C ~ 26 DEG C, and suitable water temperature is 20 DEG C ~ 24 DEG C, uses oxytocic drug to hasten parturition, then carries out natural insemination or artificial insemination; Incubating oosperm, to fry membrane, is then put into and is cultivated pond by meeting fry that parent population breeding that onychostoma simus biology economic characters require obtains and support temporarily, cultivate into the fry that total length is 1.5 ㎝ ~ 2.0 ㎝, then proceed to pond and carry out seed rearing until fingerling goes out pond.
2. onychostoma simus artificial breeding method as claimed in claim 1, is characterized in that, the selection of described milter: kissing the upper jaw mating season has and coarsely chase after the rough hand of raw meat, and belly lateral deviation is gently pressed with seminal fluid and flows out external, gonapophysis end blush; The selection of raun: mating season belly expand, soft, ovary profile is obvious, gonopore relaxes, and have flowing shape after ovary tenesmus, anus is red and swollen, and dig ovum inspection, ovum grain is full, and size is neat, glossy, in buff; Most core off normal is checked by transparent liquid.
3. onychostoma simus artificial breeding method as claimed in claim 1, it is characterized in that, described oxytocic drug comprises carp brain hypophysis (PG), pimozide DOM (DOM), fish is with human chorionic gonadtropin (HCG), fish is with luteotropin releasing hormone d-ala analog (LRH-A), alepidote hormone and fish efficient induced spawning mixture, oxytocic drug injection system adopts pectoral fin base portion or muscle of back injection, raun oxytocic drug accumulated dose is injected at twice, injected dose is 1/8 ~ 1/10 of accumulated dose for the first time, second time injection is carried out after the 10h ~ 16h of interval, injected dose is whole surplus, milter disposable injection when the injection of raun second time, its drug dose is 1/3 ~ 1/2 of raun accumulated dose.
4. onychostoma simus artificial breeding method as claimed in claim 1, it is characterized in that, the concrete grammar of natural insemination is as follows: spawning pond cleans up, and arranges a brown sheet laying nest, press solidly with gravel bottom pond, add water to 0. 6m ~ 0.8m dark, press 1:1 combo after parent population second time injection ocyodinic, put into spawning pond, support temporarily with miniflow water, produce after ovum until parent population and pulled fish nest out, be suspended on Lotic hatching in hatching pail; The hatching density being suspended on Lotic hatching in hatching pail is 2.5 × 104, each hatching pail water body; Environment of hatching should meet the following conditions: water quality should meet the regulation of GB 11607, wherein the dissolved oxygen amount of water body should at 6 ㎎/more than L, and pH value is 6.8 ~ 8.2, keep water quality pure and fresh, Spawning water specification is that 24 Kong/㎝ polyamide fibres or terylene mesh filter, and is strictly on guard against that harmful organisms enters; Hatching water temperature range is 20 DEG C ~ 26 DEG C, and suitable water temperature is 22 DEG C ~ 24 DEG C.
5. onychostoma simus artificial breeding method as claimed in claim 4, it is characterized in that, by the regulation unsticking of SC/T 1013 after natural insemination, the fertilized egg after unsticking puts into hatching pail Lotic hatching until incubating oosperm is to fry membrane, and the hatching density of unsticking ovum in hatching pail is 30 × 104; Incubating oosperm is as follows to the required time of fry membrane:
Water temperature and fry membrane time relationship
6. onychostoma simus artificial breeding method as claimed in claim 4, is characterized in that, described cultivation pond adopts flowing water cement pit; Its shape is rectangle, and area is 3 ㎡ ~ 5 ㎡, and the depth of water is 50 ㎝ ~ 60 ㎝; The screen cloth screen window of discharge outlet aperture 6mm prevents fry from fleeing, and flowing water cement pit lucifuge object is covered lucifuge condition; Put Miao Qianying by the clear pond sterilization of the regulation of SC/T 1008; Cultivate the regulation that pond water quality should meet GB 11607, water quality is pure and fresh, and wherein water body dissolved oxygen amount should be 6.8 ~ 8.5 at 6 ㎎/more than L, pH, and transparency is 40 ㎝ ~ 45 ㎝, and suitable water temperature is 20 DEG C ~ 28 DEG C, and fry puts into water temperature difference when cultivating pond and is no more than ± and 2 DEG C; Every cubic metre of water body puts 2 × 104 tail ~ 3 × 104 tails, treat that fry can freely move about and fish body is covered with melanin, after yolk sac major part absorbs, start first to open food with yolk water liquid, to throw something and feed again living body biological bait wheel animalcule, cultivate into the fry that total length is 1.5 ㎝ ~ 2.0 ㎝; Flowing water pond should keep uninterrupted flowing water, is changed once and blowdown by the water body in flowing water pond about 80% every 2d, or with aerator to water oxygenation; Directly to throw something and feed living body biological bait wheel animalcule in cultivation pond when fry starts to ingest.
7. onychostoma simus artificial breeding method as claimed in claim 6, is characterized in that, will support that to cultivate into total length be that the fry of 1.5 ㎝ ~ 2.0 ㎝ is dropped into pond and carries out nurture of fish fingerlings temporarily cultivating pond, and fry can ingest zooplankton; Fry neat specification, body colour are consistent, travelling active, anosis, nothing wound, nothing deformity; Outside the regulation of pond by SC/T 1008, pond area is 1 500 ㎡ ~ 2 000 ㎡, and pond shape is neat, and smooth at the bottom of pond, silt depth 6 ㎝ ~ 10 ㎝, has abundant water resources, pollution-free source, and irrigation and drainage are convenient; The sterilization of seed rearing pond should meet the regulation of SC/T 1008 and NY 5071 respectively with cultivating quality of water, after cultivating a large amount of zooplankton and transparency and being 45 ㎝, throw in fry again; The breeding density of fry with go out pond specification and see the following form:
Fry breeding density with go out pond specification
CN201310578354.XA 2013-11-19 2013-11-19 Artificial breeding method for onychostoma simus Pending CN104642212A (en)

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Cited By (12)

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Publication number Priority date Publication date Assignee Title
CN105029110A (en) * 2015-08-31 2015-11-11 青岛海之源智能技术有限公司 Mixed feed for onychostoma simum and preparing method of mixed feed
CN105076833A (en) * 2015-08-31 2015-11-25 青岛海之源智能技术有限公司 Compound feed for juvenile fish of onychostoma simum and preparation method of compound feed
CN105124251A (en) * 2015-08-31 2015-12-09 青岛海之源智能技术有限公司 Fish feed capable of preventing tail rot disease of onychostoma simus and preparation method of fish feed
CN105724291A (en) * 2016-03-07 2016-07-06 五河县金满塘生态种养殖家庭农场 Artificial breeding method for soft-shelled turtles
CN107333680A (en) * 2016-04-29 2017-11-10 岚皋县绿水生态农业有限责任公司 A kind of artificial breeding method of more squama shovel jaw fishes and special hatchery
CN106359195A (en) * 2016-09-21 2017-02-01 罗平美丽家园水产有限公司 Breeding method for fingerlings of Onychosotoma gerlachi
CN106417105A (en) * 2016-09-21 2017-02-22 罗平美丽家园水产有限公司 Fish-fry breeding method for semilabeo obscurus
CN106417105B (en) * 2016-09-21 2019-11-05 罗平美丽家园水产有限公司 A kind of fry rearing method of dead color lip dace
CN106359195B (en) * 2016-09-21 2019-11-05 罗平美丽家园水产有限公司 A kind of offspring breeding method of vacricorhinus gerlachi
CN112616731A (en) * 2020-12-30 2021-04-09 铜仁市渔业技术推广站 Small white soft-shelled turtle bionic breeding pond and breeding method
CN112616729A (en) * 2020-12-30 2021-04-09 铜仁市渔业技术推广站 Artificial propagation method of small-mouth white soft-shelled turtles
CN113424782A (en) * 2021-07-06 2021-09-24 湖北中水长江生态保护研究院有限公司 Artificial propagation method for white soft-shelled turtles

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