CN103933346B - A kind of compound for reducing blood suger and preparation method thereof - Google Patents

Abstract

The invention discloses a kind of compositions with YIN nourishing reduction blood glucose function having plant material to combine and preparation method thereof, including Cortex Lycii, Fructus Lycii, Fructus Ligustri Lucidi, Rhizoma Polygonati Odorati, Rhizoma Dioscoreae, Radix Ophiopogonis, Semen Cassiae, the nourishing-yin, lowering blood-sugar compositions of the present invention can be prepared to any conventional dosage form for oral administration, to treating and preventing the evident in efficacy of diabetes, the total effective rate of diabetes is reached more than 90%, and after drug withdrawal no matter fasting glucose or 2h-plasma glucose all without bounce-back, the nourishing-yin, lowering blood-sugar compositions side effect of the present invention is little, spleen benefiting and strengthen kidney, nourishing YIN and invigorating YANG, the effects such as mediation the five internal organs, treating both the principal and secondary aspects of a disease, medicine can promote pancreatic cell regeneration after entering body, strengthen the secretion of insulin, internal carbohydrate metabolism can be improved, and the multiple complications causing diabetes has good prevention effect.

Description

A kind of compound for reducing blood suger and preparation method thereof

Technical field

The present invention relates to a kind of Chinese medicine blood sugar lowering and the blood sugar lowering of health care and the protection of complication prevention.

Background technology

Diabetes, also known as diabetes, are the metabolic diseases of a kind of multi-pathogenesis, can betide any age and with always Year, people was in the majority, and according to Epidemiological study, whole world diabetes patient there are about 2.3 hundred million at present, and China just accounts for 4000 Ten thousand, and increase with surprising rapidity every year.The harm of diabetes is essentially from diabetic complication, along with the course of disease Prolongation, the histologic lesion such as easily concurrent whole body is neural, microcirculation and large artery trunks vascular lesion, and may result in the heart, The chronic progressive external pathological changes of the histoorgans such as liver, kidney, eye.Become and seriously endangered after cardiovascular and cerebrovascular vessel, cancer The another major disease of evil human health.

Traditional Chinese medical science traditional medicine thinks that the reason of induced Diabetic is due to the imbalance of liver,spleen,kidney functional metabolism, gastrointestinal Damp and hot gather caused by surplus, symptomatic reaction with xerostomia, thirsty, hungry, become thin, frequent micturition, gluttony, crapulence etc.. Western medicine is considered to become estranged due to human insulin secretion or to act on sugar, fat and protein metabolism that defect causes disorderly Disorderly etc..

According to statistics, the drug main for the treatment of diabetes to account for the market share nearly 74%, Chinese medicine based on Western medicine at present Only account for 26%, but the side effect of Western medicine is big, costly, and blood glucose can only be played control action, but cannot Cure diabetes, and raw material of Chinese medicine by scientific compatibility index blood-sugar lowering tcm drug, organ function can be adjusted Balance, has treating both the principal and secondary aspects of a disease, and the feature such as have no side effect, and can effectively reduce blood sugar content, reaches diabetes To the purpose cured, effectively protect diabetics healthy, and avoid the generation of diabetic complication, therefore The Chinese medicine protection diabetics developing the effective treatment and health of a kind of function is healthy, has certain society and economy Benefit.

Summary of the invention

The primary and foremost purpose of the present invention be for the problems referred to above provide a kind of have reduce blood glucose function compositions and Its preparation method, the present composition is for because of reasons such as overnutrition and organ function disorder, endocrine disturbance The cause of disease of caused diabetes, uses plurality of Chinese to cooperate, and the mutual Synergistic of its active component has Effect ground reduces blood glucose, and the present composition is high to the curative effect of prevention and treatment diabetes, without any side effects, And blood glucose value does not rebounds after drug withdrawal.

For realizing the purpose of the present invention, one aspect of the present invention provides a kind of compositions with lowering blood-fat and reducing weight function, Including following raw material: Cortex Lycii, Fructus Lycii, Fructus Ligustri Lucidi, Rhizoma Polygonati Odorati, Rhizoma Dioscoreae, Radix Ophiopogonis, Semen Cassiae.

Wherein, the weight described in the composition material reducing blood glucose function is: Cortex Lycii 15-35, Fructus Lycii 15-50, Fructus Ligustri Lucidi 10-50, Rhizoma Polygonati Odorati 10-35, Rhizoma Dioscoreae 10-35,10-30 Radix Ophiopogonis, Semen Cassiae 10-30.

Particularly, the weight of described crude drug is preferably: Cortex Lycii 20-30, Fructus Lycii 25-40, female Loyal sub-20-40, Rhizoma Polygonati Odorati 15-25, Rhizoma Dioscoreae 10-30,15-25 Radix Ophiopogonis, Semen Cassiae 15-20.

Especially, the weight of described crude drug is: Cortex Lycii 20, Fructus Lycii 30, Fructus Ligustri Lucidi 30, Rhizoma Polygonati Odorati 20, Rhizoma Dioscoreae 20, Radix Ophiopogonis 20, Semen Cassiae 20.

The blood glucose compositions that reduces of the present invention is to fully realize the pathogenetic of diabetes based on Chinese medicine, knot Closing modern pharmacy and the achievement of pharmaceutical research, selectively Cortex Lycii, Fructus Lycii, Fructus Ligustri Lucidi, Rhizoma Polygonati Odorati carry out science Prescription is prepared from, and with the Chinese crude drug of pure natural as raw material, has no side effect, it by nourishing YIN and clearing away heat, promote the production of body fluid Quench the thirst and clofibrate sugar etc., reach to treat and prevent diabetes and the purpose of diabetic complication.

Wherein, Cortex Lycii another name Chinese wolfberry skin, for the root bark of Fructus Lycii Lycium chinense Mill..Cold in nature, taste Sweet, return liver, kidney, lung meridian.There is removing heat from blood except steaming, effect of lung heat clearing pathogenic fire reducing, be used for treating deficiency of YIN hectic fever, bone Steam night sweat, cough due to lung-heat, spitting of blood, epistaxis, stranguria with blood, quench one's thirst, hypertension, carbuncle, malignant boil, asthenia hectic fever, Night sweat, diabetes.The chemical composition of Cortex Lycii is mainly containing cinnamic acid and volume aldehydes matter, glycine betaine, Ke's alkali A (Kukoamine), Fructus Lycii element A, Fructus Lycii element B, cupreol, linoleic acid, linolenic acid etc..Research table The alkaloid contained in bright Cortex Lycii such as glycine betaine, hardship can amine, for immunomodulating, agent, antiviral, The 1 of tumor inhibitor etc., 2,3,4,7 ,-penta hydroxy group-6-azabicyclo [3,3,0] octane and Isosorbide-5-Nitrae, 7, 8-tetrahydroxy-6-azabicyclo [3,3,0] octane, anti-feritin effect hold concurrently antiangiotensin I converting enzyme activity Fructus Lycii ring octapeptide A and B of effect and the organic acid of antiangiotensin I converting enzyme active function.

Modern pharmacological research shows that Cortex Lycii has the effect of blood sugar lowering, blood fat reducing.Cortex Lycii decoct is gavaged to rabbit, First make the blood glucose short time raise, the most persistently reduce, persistently reduce and reach more than 4～8 hours.For injection The hyperglycemia that epinephrine causes there is no obvious antagonism, but can shorten hyperglycemia and hold time. and rabbit is subcutaneous Cortex Lycii extractum parenterally, also can make blood glucose reduce.Cortex Lycii water decoction is to alloxan diabetes mouse islets B The morphosis infringement of cell has and certain alleviates effect.Cortex Lycii extractum gavage, can make Experimental Rabbit serum Total cholesterol level declines, but little, to hepatic fat content also without obvious shadow on content of triglyceride impact Ring.Glycine betaine then has lipotropy.Rabbit is gavaged continuously Cortex Lycii decoct 3 weeks (consumption 10g/kg/ day), Its serum total cholesterol content can be made to decline 37%, and there is certain lipotropic effect, and without appointing What toxicity.

Fructus Lycii is the dry mature fruit of plant of Solanaceae lycium barbarum, and property is put down, and sweet in the mouth returns liver, kidney channel.Tool There is nourishing the liver and kidney, the function of replenishing vital essence to improve eyesight, it is mainly used in asthenia damage of essence, soreness of waist and knee joint, vertigo and tinnitus, interior-heat Quench one's thirst, blood deficiency and yellow complexion, blurred vision is failed to understand, passes out semen, night sweat, blood deficiency yellowish complexion, neurasthenia, hepatitis, carbuncle, Chilblain, scalds, decubital ulcer, chronic atrophic gastritis, stomach and duodenal bulbar ulcer, is used for treating Liver and kidney cloudy Lose, soreness of the waist and knees, have a dizzy spell, cough due to consumptive disease, quench one's thirst seminal emission, blood deficiency and yellow complexion, neurasthenia etc...

Containing containing carotene, thiamine, riboflavin, nicotinic acid, ascorbic acid, Radix Betae in the fruit of Fructus Lycii Alkali, atropine, hyoscyamine, physalin etc.；Containing aminoacid in the seed of Fructus Lycii: aspartic acid, Proline, alanine, leucine, phenylalanine, serine, glycine, glutamic acid, cysteine, rely Propylhomoserin, arginine, isoleucine, threonine, histidine, tyrosine, tryptophan, methionine etc..

Modern pharmacological research shows that Fructus Lycii has lipotropic effect, the infusion (20%, 8 of Fructus Lycii Ml day gavage), to the mice poisoned by carbon tetrachloride, there is suppression fat to deposit in hepatocyte, promote The effect that hepatocyte is newborn.The lipotropic effect of water extract is also manifested by, and prevents carbon tetrachloride from causing Liver dysfunction.Fructus Lycii is to lipid metabolism or lipotropic effect, mainly due to glycine betaine contained therein Caused, glycine betaine plays the effect of methyl supplying in vivo.Lycium barbarum polysaccharide to mice nonspecific immunity and Specific immunity has significant immunostimulant and regulation effect, reduces blood glucose, the effect of blood fat, it is possible to Slow down aging and the effect of resisting fatigue.Experimental study proves: Fructus Lycii can be effectively improved hepatic and renal function and have Immunoloregulation function and defying age, rush hemopoietic, blood fat reducing and protect the liver, lipotropic effect, and have notable Blood sugar decreasing effect, its mechanism of action is, contained guanidine derivatives can effectively suppress animal blood glucose to raise, And make animal that the tolerance of carbohydrate to be improved.

Fructus Ligustri Lucidi is the dry mature fruit of Oleaceae plants Fructus Ligustri Lucidi, cool in nature, sweet in the mouth, micro-hardship, returns liver, kidney channel. There is nourishing the liver and kidney, crow goal of the invention effect, be mainly used in vertigo and tinnitus, soreness of the waist and knees, early whitening of beard and hair, mesh Dark not clear.For treat the hepatic and renal YIN deficiency, interior-heat Light-headedness, tinnitus dim eyesight, soreness of the waist and knees, early whitening of beard and hair, Glaucoma, habitual constipation, chronic bronchitis, cerebral edema, renal failure, angina pectoris, ascites, hat Cardiopathia etc..The chemical composition of Fructus Ligustri Lucidi mainly contains triterpenoid compound, nuzhenide, oleuropein, neat Pier fruit acid, acetyloleanolic acid, ursolic acid, acetic acid ursolic acid, 4-hydroxyl-B-phenethyl-B-D-glycoside, The acid of betulinol, Quercetin, eigustroside, new nuzhenide, eigustroside, cleupin acid, flavone, glucosides and Trace elements K, sodium, calcium, zinc, ferrum, copper, manganese etc..

Pharmacological research shows: Fructus Ligustri Lucidi has the function of blood fat reducing and arteriosclerosis: Fructus Ligustri Lucidi powder admixes foodstuff Middle feed, can reduce serum ornitrol and content of triglyceride to Experimental Hyperlipemia disease rabbit, and make aorta fat Matter speckle and Atherosclerotic Vessels: Changes Observed during Coronary form abatement；Chemical composition oleanolic acid 30mg/kg in Fructus Ligustri Lucidi Admixing beverage feed rat, 0.4% oleanolic acid suspension 0.5ml is to rabbit gavage, to hyperlipidemia rat, rabbit All there is effect for reducing blood fat；Oleanolic acid 30,60mg/kg are added on feed Japanese quail 8 weeks in feedstuff, hence it is evident that fall The total cholesterol of low serum, lipid peroxide, the total cholesterol content of arterial wall, reduce atherosclerotic generation Rate；Fructus Ligustri Lucidi also has blood sugar lowering, anti-liver injury and improves the effect of body's immunity；Promote that body is made The Cardiovascular Systems such as blood, heart tonifying, expansion coronary vasodilator, expansion peripheral blood vessel；Diuresis, cough-relieving, cathartic, Antibacterial grade acts on.Fructus Ligustri Lucidi plant has prevention and therapeutical effect to the diabetes mice causing alloxan, also The mouse blood sugar that can cause antiadrenergic drug or glucose raises.

Rhizoma Polygonati Odorati is the dry rhizome of liliaceous plant Rhizoma Polygonati Odorati Polygonatum odoratum (Mill.) Druce, Cold in nature, sweet in the mouth, returns lung stomach warp, has effect of nourishing YIN to relieve dryness, promoting the production of body fluid to quench thirst, hinders for lung stomach-Yin, scorching Cough, dry throat and mouth, interior-heat is quenched one's thirst.It is mainly used in treating consumption of YIN caused by febrile disease, cough excessive thirst, asthenia interior-heat, tired Thirsty easily famine, frequent micturition etc..Root stock mucopolysaccharide Han Rhizoma Polygonati Odorati, by D-Fructose, D-MANNOSE, D-Glucose And galacturonic acid is formed, mol ratio is 6:3:1:1.5；Rhizoma Polygonati Odorati levan A, B, C, D, sugar Consist of fructose, glucose, AzeOH, Rhizoma Polygonati spiral shell sterol Poa, Rhizoma Polygonati spiral shell sterol glycoside Pob, Poc, PO1, PO2, PO3, PO4, PO5, Rhizoma Polygonati furan sterol glycoside, Rhizoma Polygonati furan sterol glycoside Poc, Pod, The steroids such as PO6, PO7, PO8 and PO9.PO2 Yu Pob has identical structure, be 25-R, S three-dimensional heterogeneous mixture, simply R/S ratio is different；PO3 is the mixture of Poc extremely 25-R.

Rhizoma Polygonati Odorati has raising immunity, promotes antibody tormation and the hypoglycemic effect of heart tonifying, defying age and fall.Give fiber crops Liquor-saturated intravenous rabbit injects 20% Rhizoma Polygonati Odorati decoct, 1-5ml, all makes blood pressure slowly increase.100% Rhizoma Polygonati Odorati injection, 5% or 10% Rhizoma Polygonati Odorati stem and leaf preserved material, 10% or 100% Rhizoma Polygonati Odorati stem and leaf decoct and 10% Rhizoma Polygonati Odorati root extractum (Rhizoma Polygonati Odorati ground Preparation prepared by lower stem), anesthetized dog, rabbit all there are is of short duration hypotensive effect.20% Rhizoma Polygonati Odorati decoct or Rhizoma Polygonati Odorati tincture, Making heart contraction strengthen isolated frog heart low dose of (2-5 drips), amplitude strengthens, and heavy dose of (10) make the heart Fight and weaken and stop rapidly.The steroid glycoside that Rhizoma Polygonati Odorati contains, the effect to cardiac muscle is similar with Herba Convallariae preparation.Rabbit with 0.5g/kg preserved material intramuscular injection, can make blood glucose rise；And its extractum is administered orally, blood glucose is fallen after rising.Add rattan sincere Report, the water portion of the methanolic extract of Rhizoma Polygonati Odorati and n-butanol fraction are to inducing glycosuria mice respectively at 4 hours After make blood glucose produce significant decline.The Rhizoma Polygonati Odorati methanolic extract blood glucose to epinephrine induced hyperglycemia mice Value has obvious reduction effect.The mechanism of action is to inhibit liver glycolysis system.20% Rhizoma Polygonati Odorati decoct, can make The significant minimizing of blood vessel perfusion flow.100% Rhizoma Polygonati Odorati (Yuquan, northeast product) injection has expansion to Bufo siccus lower limb vascular Effect.Isolated rabbit ear vessls experiment shows that 100% Rhizoma Polygonati Odorati injection 0.4ml/kg is noted by tube for transfusion Enter, distend the blood vessels.

Rhizoma Dioscoreae is also known as Rhizoma Dioscoreae, for the dry rhizome of Dioscoreaceae plant Rhizoma Dioscoreae.(Jiaozhuo, modern Henan Province, Gu Huai celebrating mansion City is domestic) Rhizoma Dioscoreae that produced is one of four-Huai medicine.Property flat, sweet in the mouth, return lung, spleen, kidney channel.There is spleen invigorating Tonifying the lung, stomach reinforcing the kidney invigorating, benefit of reinforcing the kidney essence, hearing-improing and eyesight improving, help the five internal organs, bone and muscle strengthening, long will to calm the nerves, life lengthening Effect.Being mainly used in treating weakness of the spleen and stomach, diet reduces, and suffers from diarrhoea in loose stool；Women's lukorrhagia due to hypofunction of the spleen；The deficiency of the lung is long Cough dry pharynx；Emission due to deficiency of the kidney.Rhizoma Dioscoreae tuber containing diosgenin, dopamine, hydrochloric acid Rhizoma Dioscoreae alkali, polyphenol oxidase, Allantoin, only wooden fork element II, glycoprotein, by mannose, glucose (glucose) and galactose in molar ratio 6.45:1:1.26 constitute Chinese yam polysaccharide, again baric, beryllium, cerium, cobalt, chromium, copper, gallium, lanthanum, lithium, Manganese, niobium, nickel, phosphorus, strontium, thorium, titanium, vanadium, yttrium, ytterbium, zinc, zirconium and sodium oxide, potassium oxide, oxidation Aluminum, ferrum oxide, calcium oxide, magnesium oxide etc..Rhizome is containing dopamine, catecholamine and cholesterol, Ergota steroid Alcohol, campesterol, stigmasterol, cupreol etc..

Modern pharmacological research shows that Rhizoma Dioscoreae Aqueous extracts can significantly inhibit the Cu2+ Denaturation to gamma Globulin, Show that there is antarthritic effect.Rhizoma Dioscoreae Aqueous extracts also can eliminate urine protein, has suppression to mutant cell and produces Raw effect.Rhizoma Dioscoreae is because planting trace element, and other mineral rich in 18 kinds of aminoacid and more than 10, so having Strengthening the spleen and stomach, tonifying the lung kidney, invigorating the spleen and replenishing QI, spleen invigorating qi-restoratives, benefit of the reinforcing the kidney effect such as smart, beneficial heart and tranquilizing mind, Rhizoma Dioscoreae rich in Multivitamin, aminoacid and mineral, can prevent and treat Human Lipid Metabolism abnormal, and arteriosclerosis, right Safeguard that insulin normal function also has certain effect, have enhancing body immunity, benefit heart and tranquilizing mind, rather cough Dingchuan, The health-care effects such as slow down aging.Rich in a large amount of protein, vitamin B group, vitamin C, vitamin in Rhizoma Dioscoreae E, glucose, crude protein aminoacid, bile alkaline, allantoin etc.；The most important nutritional labeling Rhizoma Dioscoreae soap is The precursor materials of synthesis estrogen, nourishing YIN and invigorating YANG, strengthens metabolic effect；And fresh tuber contains The phlegmatic temperament of some GL-PP compositions, zymigen etc., can prevent cadiovascular lipidosis, contribute to gastrointestinal Digestion and absorption.

Radix Ophiopogonis is liliaceous plant Radix Ophiopogonis (dwarf lilyturf) Ophiopogon japonicus (Thunb.) Ker-Gawl. Dried root.Cold nature, sweet in the mouth, micro-hardship；GUIXIN, lung, stomach warp.Having YIN nourishing and the production of body fluid promoting, lung moistening clears away heart-fire. Being mainly used in treating dryness of the lung dry cough, chronic consumptive disease is coughed, and Tianjin wound is thirsty, vexed insomnia, and interior-heat is quenched one's thirst, dryness of the intestine constipation； Pharyngeal diphtheria, few thirsty, dry cough spitting of blood for Tianjin of lung-stomach yin deficiency；The palpitation and being susceptible to fright of insufficiency of heart-YIN and calentura later stage Consumption of body fluid by heat etc. are demonstrate,proved.Contain multiple steroid saponin Radix Ophiopogonis: Radix Ophiopogonis Saponin A, B, C, D, glycoside unit is phyllodium Tree ruscogenin, separately containing ophiopogonin B ', C ', D ', glycoside unit is diosgenin；Still containing multiple flavonoid Compound: such as methyl yellow alkanone A Radix Ophiopogonis, B, Radix Ophiopogonis flavanone A, ophiopogonone A, B, methyl is yellow for Radix Ophiopogonis Ketone A, B；Separately get 5 homoisoflavone compounds.

Modern pharmacological research shows that Radix Ophiopogonis, decoct had sedation, also can strengthen the sedation of chlorpromazine, strengthens The syngignoscism of pentobarbital sodium, the excitation of antagonism caffeine, the tic that dimefline causes can be postponed, by force Straightforward convulsions and the dead time occurred, but animal can not be made to avoid death.Ophiopogon root total saponin I (crude extract) Strengthen the effect of Bufo siccus myocardial contraction, and Total saponin II (purer thing) effect is not as good as Total saponin I, typically exists With kinemic increase while myocardial contraction enhancing.Heavy dose of Total saponin I, II and total sugar are to the heart Dirty all produce suppression, myocardial contraction can be made to weaken, cardiac output reduces, atrioventricular block, even stop Fu. Mice adds root hair Radix Ophiopogonis in feedstuff can reduce internal hydroxyproline.To monoamine oxidase, MAO in male mice brain (MAO-B) suppression ratio is 38.6%.In male mice liver, SOD activity improves 45.5%.Life span of drosophila melanogaster Test also shows that root hair feedstuff Radix Ophiopogonis has and significantly extends life span of drosophila melanogaster, and prompting has slow down aging trend.Swimming examination Test and show that Saponin contained by Radix Ophiopogonis, polysaccharide, aminoacid etc. have obvious antifatigue effect.

Semen Cassiae is pulse family annual herb plant Semen Cassiae or the dry mature seed of little Semen Cassiae, cold nature, bitter in the mouth； Return liver, kidney, large intestine channel.There is clearing away heat to improve acuity of vision, effect of loosening bowel to relieve constipation, it is mainly used in the puckery pain of conjunctival congestion, photophobia Many tears, dizziness of having a headache, poor vision, constipation.There is effect of fat-reducing.Control wind heat acute conjunctivitis, optic atrohic blindness, passeris montani saturati Mesh, hypertension, hepatitis, cirrhotic ascites, habitual constipation etc..The chemical composition of Semen Cassiae mainly contains greatly Flavin, chrysophanol, physcione, obtusin, obtusin and glycoside thereof；Possibly together with multivitamin With abundant aminoacid, fat, carbohydrate etc., its health care is increasingly subject to the attention of people.

Pharmacological research shows that Semen Cassiae alcohol extraction thing is to staphylococcus, diphtheria corynebacterium, Bacillus typhi, paratyphoid fever bar Bacterium, escherichia coli all have inhibitory action；Semen Cassiae water logging agent and ethanol immersion all have fall to anesthesia Canis familiaris L., cat, rabbit Pressure effect.Semen Cassiae decoct or 6g/ feed rat of Trial On The Action of Semen Cassiae Powder 8 weeks are big to Experimental Hyperlipemia disease Mus can reduce blood plasma ornitrol, triglyceride, and reduces the content of triglyceride in liver.Semen Cassiae decoct or scattered Agent 6g/kg feed rat 8 weeks, has inhibitory action to the rat platelet aggregation of ADP induction.Semen Cassiae heat Water extract is administered orally 670mg/kg has weak Detoxication to carbon tetrachloride poisoning mouse liver.The first of Semen Cassiae Alcohol extraction thing has significant liver protection effect.

Another aspect of the present invention provides the preparation method of a kind of compositions having and reducing blood glucose function, including as follows Step:

1) according to following weight preparation raw material:

Cortex Lycii 15-35, Fructus Lycii 15-50, Fructus Ligustri Lucidi 10-50, Rhizoma Polygonati Odorati 10-35, Rhizoma Dioscoreae 10-35, Radix Ophiopogonis 10-30, Semen Cassiae 10-30；

2) by after described crude drug mix homogeneously, mixing crude drug is carried out heating extraction, collects extracting solution；

3) extracting solution collected is carried out concentration, prepare concentrated solution；

4) concentrated solution is dried process, to obtain final product.

Wherein, step 1) described in crude drug weight be preferably: Cortex Lycii 20-30, Fructus Lycii 25-40, Fructus Ligustri Lucidi 20-40, Rhizoma Polygonati Odorati 15-25, Rhizoma Dioscoreae 10-30,15-25 Radix Ophiopogonis, Semen Cassiae 15-20；Excellent Elect Cortex Lycii 20, Fructus Lycii 30, Fructus Ligustri Lucidi 30, Rhizoma Polygonati Odorati 20, Rhizoma Dioscoreae 20, Radix Ophiopogonis 20, Semen Cassiae 20 as.

Wherein, step 2) described in heating extraction include the step that order below is carried out:

A) feeding the mixture into water soaking, the weight of the water of addition and the weight ratio of mixture are 6-10:1, Carrying out heating extraction for the first time after soaking 1-3 hour, heating-up temperature is 100 DEG C, is 100 DEG C keeping temperature Under conditions of extract 1-3 hour；

B) first time extracting solution is filtered, collect filtrate；

C) in filtering residue add water, carry out second time heating extraction, wherein, the weight of the water of addition with mix The weight ratio of thing is 5-10:1, and heating-up temperature is 100 DEG C, extracts under conditions of keeping temperature to be 100 DEG C 1-3 hour；

D) filter, the filtrate of twice filtration is merged.

Particularly, step A) described in 1.5-2.5 hour heating extraction time, the weight of the water of addition is with mixed The weight ratio of compound is preferably 7-8:1；Step C) in the weight ratio of weight and the mixture of water that adds For 5-7:1.

Wherein, step 3) described in filtrate concentration during control temperature and be 80-100 DEG C, relatively press Power is less than-0.05MPa.

Particularly, described relative pressure is preferably-0.05～-0.095MPa.

Particularly, step 3) described in weight and the crude drug Cortex Lycii of concentrated solution, Fructus Lycii, Fructus Ligustri Lucidi, Rhizoma Polygonati Odorati, Rhizoma Dioscoreae and Radix Ophiopogonis, the ratio of gross weight of Semen Cassiae are 0.4-0.7:1, preferably 0.5-0.6:1, enter One step is preferably 0.6:1.

Wherein, dried described in step 4 is spray drying, lyophilization.

Particularly, also include step 5) compositions after dried adds pharmaceutically acceptable adjuvant, Mix.

Particularly, after adding adjuvant dextrin and food preservative mix homogeneously, adding appropriate distilled water, be placed in Pellet processing machine is made pill.

Especially, the compositions after described dried is 10:100 with the weight proportion of dextrin；Described dry Compositions after process is 100:0.05 with the weight ratio of food preservative.

Particularly, described food preservative select potassium sorbate, sodium diacetate, benzoic acid and salt thereof, calcium propionate, Sodium lactate or dehydroactic acid sodium.

Wherein, described pharmaceutically acceptable carrier is generally used for this purpose and conduct by sanitarian's accreditation The non-active ingredient of medicament.Compilation about pharmaceutically acceptable carrier can be at " drug excipient hands Volume " (Handbook of Pharmaceutical excipients, second edition, by A.Wade and P.J. Weller edits；American Pharmaceutical Association publishes, Washington and The Pharmaceutical Press, London, 1994) etc. reference book finds.

Particularly, described carrier includes excipient, such as starch, water etc.；Lubricant, such as magnesium stearate etc.； Disintegrating agent, such as microcrystalline Cellulose etc.；Filler, such as lactose etc.；Binding agent, such as pregelatinized Starch, paste Essence etc.；Sweeting agent；Antioxidant；Preservative, correctives, spice etc..

The active component of medicine of the present invention can add various customary adjuvant required when preparing different dosage form, as collapsed Solve agent, lubricant, adhesive etc. and be prepared as any conventional peroral dosage form with conventional method of Chinese medicinal, Such as pill, powder, tablet, granule, capsule, oral liquid etc..

The present invention reduces blood glucose compositions just according to function and the chemical composition spy to human body of above medicine Different effect compatibility forms, for diabetes clinical symptoms xerostomia, thirsty, polyphagia, the most tired, gluttony, crapulence, Frequent micturition, weak, visual deterioration, immunologic function degression etc., according to theory of Chinese medical science, suit the remedy to the case, be with ground bone Skin removing heat from blood is except steaming, resolving heat and reducing pathogenic fire；Fructus Lycii, the nourishing the liver and kidney of Fructus Ligustri Lucidi, nourishing blood for improving eyesight；Rhizoma Polygonati Odorati is nourshing Yin and drynsessmoistening prescription, Medicine selected by promoting the production of body fluid to quench thirst, the famine that disappears diuresis；Radix Ophiopogonis, Semen Cassiae promoting the production of body fluid to quench thirst, nourishing YIN and clearing away heat, liver heat removing and eyesight improving. Prove all to have certain hypoglycemic according to pharmacological research, improve the effect of immunity.Improve the collaborative work of medicine With, blood of human body and liver,spleen,kidney are had benefit, has and rush down, YIN and YANG balancing, reach to improve immunologic function, promote Enter the balance of hormonal system, enhanced resistance against diseases, be effectively accomplished the purpose of disease curing and body-building.According to Proving the animal experiment of book on Chinese herbal medicine YIN nourishing benefit sugar pill, YIN nourishing benefit sugar pill not only blood sugar lowering, lipid-lowering effect are stable, definite, And long-term taking has no side effect.

Detailed description of the invention

The following is the test of the pharmacodynamic study to technical solution of the present invention, the present invention is by studying Chinese drug-treated group of the present invention The blood sugar lowering of STZ and alloxan animal hyperglycemia model is tested, to alloxan model mice blood by compound Clear insulin content and the impact of alloxan model mice anti-fatigue test, sufficiently illustrate Chinese medicine of the present invention The pharmacodynamics curative effect of compositions treatment diabetes.

Test example 1

1, test material

1-1, medicine and reagent

Prepared by 1-1-1, compound for reducing blood suger trial drug of the present invention

It is spray-dried so that embodiment 1 to be passed through, prepares compound for reducing blood suger dry powder and test.

1-1-2, control drug

1-1-2-1, the preparation of Chinese medicine composition for the treatment of diabetes of Patent No. 200710115293.8

The treatment sugar of Patent No. 200710115293.8 is prepared according to this patent specification detailed description of the invention 1 The Traditional Chinese medicine composition liquid medicine that urine is sick, specific as follows:

By crude drug Fructus Corni 20g, Rhizoma Dioscoreae 20g, Rhizoma Polygonati Odorati 15g, Fructus Ligustri Lucidi 15g, Fructus Lycii 15g, Radix Ophiopogonis Fill after 15g, Radix Trichosanthis 15g, Radix Polygoni Multiflori 15g, Cortex Lycii 20g, Fructus Amomi 5g, Radix Glycyrrhizae 10g mix homogeneously Entering in marmite, add water and soak in right amount, first intense fire is fried boiling, then decocts half an hour with slow fire, leaches medicinal liquid, in medicinal residues Add water and decoct half an hour more in right amount, then leach medicinal liquid.Twice medicinal liquid mixing；

Medicinal liquid lyophilization after merging, standby, as compareing 1 group.

1-1-2-2, positive control medicine: JIANGTANGSHU JIAONANG (Tian Tai Pharmaceutical limited company of Jilin Province；Specification: 0.3g*00 grain), compare 2 groups.

1-2, reagent

Streptozotocin (STZ): Sigma Co., USA.

Before experiment, streptozotocin is dissolved in (pH=4.2-4.3) configuration in 0.05M citric acid ice bath buffer salt solution Become 13mg/ml solution, standby.

1-3, experimental animal

Healthy Kunming mouse (male and female half and half, body weight 16-18g), the laboratory animal quality certification number: 0097976. Credit number: scxK (Guangdong) 2,008 1 0002, is provided by Guangdong Medical Lab Animal Center.

2, test method

Taking healthy mice 100, before experiment, fasting 18 hours, freely drinks water, preparation before lumbar injection experiment Streptozotocin (STZ) solution 0.2ml, dosage is 130mg/kg.Take after lumbar injection STZ solution 72h Hematometry mouse blood sugar value, selects blood glucose value >=13.8mmol/L mice as diabetic mice, chooses it In 80 points 4 groups at random, often group 20, respectively the model control group, (comparison of positive drug control group 2 groups), the Chinese medicine composition group (compareing 1 group) of the treatment diabetes of Patent No. 200710115293.8, Compound for reducing blood suger group of the present invention.

Separately take healthy mice 20, as blank group.Blank group mouse peritoneal injection same volume Normal saline.

To blank group, model control group, comparison 1 group, compare 2 groups, compound for reducing blood suger group of the present invention little Mus gastric infusion: wherein blank group and model control group give normal saline 2.0ml/100g, positive control Group gives JIANGTANGSHU JIAONANG 10mg/100g, and gives of the present invention group of medicine and the dosage of 1 group of compositions of comparison The dosage of 10mg/100g, once a day, continuous 3 weeks, and fasting 3 hours before last is administered, it is administered Within latter 2 hours, taking blood and survey blood glucose value, carry out statistical procedures, experimental result is shown in Table 1.

3, result of the test

Statistical procedures: experimental result withRepresent, use t inspection, measure the significance of group difference.

The table 1 impact on STZ model mice blood glucose

Result of the test shows: model group is compared with blank group, and the blood glucose value of the two has significant diversity Reach, show this test modeling success；Compare with model control group, JIANGTANGSHU JIAONANG, compound for reducing blood suger of the present invention Group, Patent No. 200710115293.8 matched group compositions all little to diabetes model caused by streptozotocin Mus hypoglycemic activity effect highly significant (P < 0.01)；Matched group group with Patent No. 200710115293.8 Compound compares, and the compound for reducing blood suger of the present invention and the reduction blood glucose action effect of JIANGTANGSHU JIAONANG are the most notable, reach Arrive statistical significance (P < 0.05), shown that the compound for reducing blood suger of the present invention causes mouse islets to by STZ Blood glucose caused by cell β damage raises obvious inhibitory action, can play hypoglycemic activity.

Test example 2

1, test material

1-1, medicine and reagent

Prepared by 1-1-1, compound for reducing blood suger trial drug of the present invention

It is spray-dried so that embodiment 1 to be passed through, prepares compound for reducing blood suger dry powder and test.

1-1-2, control drug

1-1-2-1, the preparation of Chinese medicine composition for the treatment of diabetes of Patent No. 200710115293.8

With the compareing 1 group and prepare identical, as compareing 1 group of test example 1.

1-1-2-2, positive control medicine: JIANGTANGSHU JIAONANG (Tian Tai Pharmaceutical limited company of Jilin Province；Specification: 0.3g*00 grain), compare 2 groups

1-2, reagent

Alloxan: Sigma Co., USA.

Alloxan is dissolved in normal saline, is configured to 60mg/ml solution for standby.

1-3, experimental animal

Healthy Kunming mouse (male and female half and half, body weight 16-18g), the laboratory animal quality certification number: 0097976. Credit number: scxK (Guangdong) 2,008 1 0002, is provided by Guangdong Medical Lab Animal Center.

2, test method

Taking healthy mice 100, before experiment, fasting 18 hours, freely drinks water, four oxygen of lumbar injection preparation Pyrimidine solution, dosage is 130mg/kg, every day lumbar injection once, inject 2d continuously.Note in last abdominal cavity Measure mouse blood sugar value after penetrating alloxan solution 72h, select blood glucose value >=13.8mmol/L mice as glycosuria Disease model mice, randomly selects wherein 80, random point 4 groups, often group 20, respectively model comparison Group, positive drug control group, Patent No. 200710115293.8 treatment diabetes Chinese medicine composition group, Compound for reducing blood suger group of the present invention.

Separately take healthy mice 20, as blank group.Blank group mouse peritoneal injection same volume Normal saline, every day lumbar injection once, inject 2d continuously.

To blank group, model control group, positive drug control group, Patent No. 200710115293.8 The treatment Chinese medicine composition group of diabetes, compound for reducing blood suger group mouse stomach of the present invention are administered: wherein blank Group and model control group give normal saline 10mg/100g, and positive controls gives JIANGTANGSHU JIAONANG 10mg/100g, and give in the treatment diabetes of of the present invention group of medicine and Patent No. 200710115293.8 The dosage of the dosage 10mg/100g of drug composition, once a day, continuous 3 weeks, and prohibit before last is administered Eat 3 hours, after administration 2 test result withRepresent, use t inspection, measure the significance of group difference.

The table 2 impact on alloxan model mice blood glucose

Result of the test shows: model group is compared with blank group, and the blood glucose value of the two has significant diversity Reach, show this test modeling success；Comparing with model control group, compound for reducing blood suger group of the present invention is to alloxan Caused diabetic mice hypoglycemic activity effect highly significant (P < 0.001), and Patent No. 200710115293.8 the blood sugar lowering curative effect slightly worse (P < 0.05) of matched group compositions；With Patent No. 200710115293.8 matched group compositions compare, the compound for reducing blood suger of the present invention and the reduction of JIANGTANGSHU JIAONANG Blood glucose action effect is the most notable, has reached statistical significance (P < 0.05), shows the blood sugar lowering combination of the present invention Thing to by alloxan cause Mice Islet Cells β damage caused by blood glucose raise have obvious inhibitory action, Play significant hypoglycemic activity.

Test example 3

1, test material

1-1, medicine and reagent

Prepared by 1-1-1, compound for reducing blood suger trial drug of the present invention

It is spray-dried so that embodiment 1 to be passed through, prepares compound for reducing blood suger dry powder and test.

1-1-2, control drug

1-1-2-1, the preparation of Chinese medicine composition for the treatment of diabetes of Patent No. 200710115293.8

With the compareing 1 group and prepare identical, as compareing 1 group of test example 1.

1-1-2-2, positive control medicine: phenformin hydrochloride tablet (Datong Li Qun Pharmaceutical Co., Ltd； Specification: 25mg), compare 2 groups

1-2, reagent

Alloxan: Sigma Co., USA.

Alloxan is dissolved in normal saline, is configured to 60mg/ml solution for standby.

1-3, experimental animal

Healthy New Zealand large ear rabbit, male and female half and half, body weight 2.5～3.0kg, by dimension tonneau China animal experimental center There is provided.

2, test method

Taking healthy rabbit 50, before experiment, fasting 18 hours, freely drinks water, four oxygen of lumbar injection preparation Pyrimidine solution, dosage is 100mg/kg, every day lumbar injection once, inject 2d continuously.Note in last abdominal cavity Measure white rabbit blood glucose value after penetrating alloxan solution 72h, select blood glucose value >=13.8mmol/L white rabbit as glycosuria Disease model rabbit, randomly selects wherein 40, random point 4 groups, often group 10, respectively model control group, Positive drug control group, the Chinese medicine composition group for the treatment of diabetes of Patent No. 200710115293.8, basis Invention compound for reducing blood suger group.

Separately take healthy rabbit 10, as blank group.Blank group white rabbit lumbar injection same volume Normal saline, every day lumbar injection once, inject 2d continuously.

To blank group, model control group, positive drug control group, Patent No. 200710115293.8 Treatment the Chinese medicine composition group of diabetes, compound for reducing blood suger group white rabbit gastric infusion of the present invention: wherein blank Group and model control group give normal saline 10mg/100g, and positive controls gives JIANGTANGSHU JIAONANG 10mg/100g, and give in the treatment diabetes of of the present invention group of medicine and Patent No. 200710115293.8 The dosage of drug composition dosage 100mg/100g, once a day, continuous 3 weeks, and fasting before last is administered 3 hours, it is administered latter 2 hours and takes blood, measure insulin content with insulin radioimmunoassay immune reagent kit, carry out Statistical procedures, experimental result is shown in Table 3.

3, result of the test

Statistical procedures: experimental result withRepresent, use t inspection, measure the significance of group difference.

The table 3 impact on alloxan model white rabbit serum insulin content

The result of the test of table 3 shows: compared with blank group, the insulin content tool of model control group white rabbit There is significant difference, show this test modeling success；To alloxan model white rabbit after three weeks treat, The compound for reducing blood suger of the present invention, the Chinese medicine composition pair for the treatment of diabetes of Patent No. 200710115293.8 Mice serum insulin content all can be made to increase, with model control group according to group, positive drug phenformin hydrochloride group Relatively, the diversity on statistical significance has all been reached；Treatment sugar with Patent No. 200710115293.8 The Chinese medicine composition matched group of urine disease compares, and in the middle of medicine of the present invention, product group and phenformin group are to alloxan mould Type mice serum insulin content dramatically increases, and reaches statistical significance (P < 0.01), and this result is strong Illustrate: alloxan model mice β cell function is recovered to have significantly promotion by the compound for reducing blood suger of the present invention Effect, reaches the effect of positive controls phenformin.

Proving according to each treated animal research experiment, the compound for reducing blood suger of the present invention has significant blood sugar lowering and makees With and the organ injuries such as the Liver and kidney that animal diabetes cause are had certain protective effect.

Test example 4 present invention reduces blood glucose compositions toxicological test

(1) acute toxicity test

The reduction blood glucose efficacy pill that has of the present invention is gavaged 1000g (crude drug)/kg to kunming mice. Any toxic reaction do not occur, one week interior without animal dead.

(2) long term toxicity test

The reduction blood glucose efficacy pill that has of the present invention is carried out long term toxicity test to rat, the most greatly Dosage (100 times of adult human dose) gastric infusion, administration time length (90 days), before and after being administered with animal General performance, body weight, food ration, hematology and Biochemical Indices In Serum, routine urinalysis, bone marrow classification, internal organs weight Amount, organ coefficient are Testing index, and are aided with animal and respectively organize the pathological examination of internal organs, overall merit medicine Toxicity profile.The indices of result display rat is the most normal.Histopathologic examination's result shows, rat Each tissue internal organs no abnormality seen.

In sum: the present invention to have reduction blood glucose efficacy toxicity low, safety range is wide, the present invention There is reduction blood glucose efficacy edible safety reliable.

Test example 5 clinical observation and curative effect

1, object of study

Choose in April, 2010 in the July, 2013 of the tested case in Dongzhimen Affiliated Hospital of Beijing University of Chinese Medicine 200 examples, use parallel control, random method for designing, are randomly divided into 4 groups, often organize 50 examples, wherein test 1 Male 26 example in group, women 24 example, year mean age (54.1 ± 1.5), the course of disease 12～32 months；Experiment 2 Male 29 example in group, women 21 example, year mean age (52.4 ± 1.8), the course of disease 12～33 months；Experiment 3 Male 27 example in group, women 23 example, year mean age (53.7 ± 1.2), the course of disease 14～35 months；Experiment 4 Male 25 example in group, women 25 example, year mean age (51.9 ± 1.7), the course of disease 13～34 months.

Each group case, in the aspects such as sex, the course of disease, age distribution there are no significant difference, has comparability.

2, case selection

Draft according to Ministry of Public Health " new Chinese medicine guideline of clinical investigations " diagnostic criteria in 1993.

1. traditional Chinese medical science diabetes diagnostic criteria: all to have thirsty polydipsia, rapid digestion of food the most hungry, frequent micturition and sweet, body are gradually shown in The disease such as become thin can be made a definite diagnosis；2. Western medicine diagnose standard: have diabetic symptom, any time blood glucose >= 11.1mmol/L or fasting glucose >=7.8m mol/L.

3, Therapeutic Method

All cases, during treating, are required to diet control, and participate in suitable physical training.Treatment group is equal The compound for reducing blood suger of the oral embodiment of the present invention 1,2 preparation, three times a day, each 5g, ante cibum is the most partly Hour take, take continuously one month.Compare 1 group and take JIANGTANGSHU JIAONANG, three times a day, be administered orally 5 every time Grain, takes one month continuously.Compare 2 groups for the treatment of diabetes that Patent No. 200710115293.8 is administered orally The compound for reducing blood suger of Chinese medicine composition, three times a day, each 5g, takes on an empty stomach, takes continuously ante cibum half an hour With one month.Treatment group, matched group withdraw other class hypoglycemic medicine during testing the most simultaneously.

Patient periodically follows up a case by regular visits at special outpatient clinic weekly, carries out clinical observation.Detect respectively before and after treatment on an empty stomach Blood glucose, 2h-plasma glucose, glycolated hemoglobin and blood fat (TC, TG), after treatment terminates, check 1 time.

4, criterion of therapeutical effect

Efficacy assessment standard: the most effective: after treatment, symptom disappears substantially, fasting glucose ＜ 7.0mmol/L, meal Rear 2 hours blood glucose ＜ 8.3mmol/L, glycolated hemoglobin and blood fat (TC, TG) laboratory indexes are the most extensive Multiple normal；The most effective: after treatment, symptom is obviously improved, fasting glucose ＜ 8.0mmol/L, 2 hours after the meal Blood glucose ＜ 10mmol/L, glycolated hemoglobin and blood fat (TC, TG) laboratory indexes make moderate progress before relatively treating, But do not recover normal；The most invalid: after treatment, symptom is not improved, blood glucose, glycolated hemoglobin and blood fat (TC, TG) not up to above-mentioned standard is declined.

5, experimental result and analysis

Before the treatment of table 4 each experimental group, after treatment 1 month, blood glucose, glycohemoglobin compare

Note: self compare with before and after treatment, * P ＜ 0.05, * * P ＜ 0.01, * * * P ＜ 0.001；With Patent No. 200710115293.8 matched group Compare after treatment, △ P < 0.05, △ △ P < 0.01.

Test result indicate that:

1, take continuously the embodiment of the present invention 1,2, the treatment diabetes of Patent No. 200710115293.8 The JIANGTANGSHU JIAONANG of Chinese medicine composition and comparison 1 group is after 1 month, and patient's fasting glucose (FPG), after the meal 2 are little Time blood glucose (2h PG), glycolated hemoglobin value, compare with before medication, have and significantly reduce and have system Meaning learned by meter, shows the treatment diabetes of compound for reducing blood suger of the present invention, Patent No. 200710115293.8 The JIANGTANGSHU JIAONANG of Chinese medicine composition and comparison 1 group has therapeutical effect in various degree to diabetes；

Compound for reducing blood suger patient's fasting glucose (FPG) that 2, prepared by the present invention, after the meal 2 hours blood glucoses (2h PG), Glycolated hemoglobin value, with the ratio of the Chinese medicine composition of the treatment diabetes of Patent No. 200710115293.8 Relatively, there is significant difference, have statistical significance, show that diabetics is had by compound for reducing blood suger of the present invention Preferably therapeutic effect；

3, the patient of 85% is taken in compound for reducing blood suger patient of the present invention after taking medicine 1 week, its fasting glucose, Take the effect of chemical classes antidiabetic drug to stomodaeum similar for 2 hours blood glucose levels after the meal；The trouble of 96% after taking medicine 2 weeks Person shows as fasting glucose, after the meal 2 hours blood glucose horizontal stables and declines, and close to normal level, and health Situation takes a favorable turn；Glucostasis after taking medicine 1 month, recovers normal level substantially.

The each experimental group of table 5 treats comparitive study after 6 months

	Treat 1 group	Treat 2 groups	Compare 1 group	Compare 2 groups
					Experimenter's quantity (example)	50	50	50	50
Effective (example)	41	40	18	14
					Effectively (example)	5	5	15	18
Invalid (example)	4	5	17	18
					Aggregate efficiency (%)	92.0	90.0	66.0	64.0

Experimental result from table 5: 1) after 6 months clinical treatments, the mouth of most of diabeticss The clinical symptoms such as dry, thirsty, polyphagia, the most tired, gluttony, crapulence, frequent micturition, weak, visual deterioration have substantially Improvement, the present invention treats 1 group, treats 2 groups, compares 1 group, Patent No. 200710115293.8 The total effective rate of the comparison 2 groups of the Chinese medicine composition for the treatment of diabetes is respectively 92.0%, 90.0%, 86.0% And 64.0%, compareing 1 group (JIANGTANGSHU JIAONANG) is 66.0%, and above clinical efficacy result illustrates: the present invention Chinese medicine composition prepared by technical scheme, in terms for the treatment of diabetes, has curing protrusion effect, and curative effect is superior to Compare the comparison 2 groups of the Chinese medicine composition of the treatment diabetes of 1 group and Patent No. 200710115293.8；2) According to the test statistics of 6 months, take the compound for reducing blood suger diabetes patient in mid-term of 6 months of the present invention continuously, Blood sugar recovery completely normal and after drug withdrawal without repeatedly accounting for 89%；Slightly 7% is repeatedly accounted for after drug withdrawal；Stop After medicine, blood glucose still instability person accounts for 4%, but the clinical symptoms of non-diabetic shows, and continues to take the fall of the present invention Sugar composite recovers normal the most substantially.

Above clinical efficacy result shows: Chinese medicine composition prepared by technical solution of the present invention is in treatment diabetes side Diabetes are especially had obvious therapeutic effect, each embodiment group medicine to be respectively provided with curing protrusion effect by face. Result shows, the Chinese medicine composition of the present invention has that preparation technology is scientific and reasonable, drug effect clearly, stable curative effect can The advantage such as leaning on, its beneficial effect is no matter from the process technique of medicine, or on the forming technique of finished product preparation, All it is effectively guaranteed that the excellent results of Chinese medicine composition of the present invention, this proves further, Chinese medicine composition of the present invention Thing is a kind of determined curative effect, safely and effectively prevents and treats diabetes medicament, has huge market potential, be worth into One step is promoted.

Further illustrate the preparation method of the present composition by the following examples.

Prepared by embodiment 1 present composition pill

1) raw material (unit: × 10g) is weighed according to following proportioning

Cortex Lycii 20, Fructus Lycii 30, Fructus Ligustri Lucidi 30, Rhizoma Polygonati Odorati 20, Rhizoma Dioscoreae 20, Radix Ophiopogonis 20, Semen Cassiae 20

2) after medical material Cortex Lycii, Fructus Lycii, Fructus Ligustri Lucidi and Rhizoma Polygonati Odorati, Rhizoma Dioscoreae, Radix Ophiopogonis, Semen Cassiae being rinsed, Be placed in Multifunctional Chinese medicine and extract in still and mix homogeneously, add tap water and soak 2 hours, wherein, addition from The weight of water is 8:1 with the ratio of the weight (dry weight) of medicinal material mixture；

3) open multi-functional extraction still power supply to heat, be heated to 100 DEG C, keep constant temperature at this temperature to carry Take 2 hours, then filter, obtain extracting solution for the first time standby；

4) in filtering residue, add the weight (dry weight) of tap water, the weight of the tap water of addition and medicinal material mixture Ratio be 6:1, heating extraction, keep heating-up temperature be at 100 DEG C extract 1.5 hours, be filtrated to get second Secondary extracting solution；

5) carry out concentrating under reduced pressure in 2 extracting solution merging being placed on vacuum high energy evaporation concentrator, prepare mixed Closing concentrated solution, wherein, the relative pressure of concentrating under reduced pressure is that-0.07MPa (i.e. with an atmospheric pressure for 0, controls Pressure during concentrating under reduced pressure is-0.07MPa relative to an atmospheric pressure), it is heated to 80 DEG C, mixed concentrated liquid Weight is 0.6:1 with the weight ratio of medicinal material mixture, i.e. carries out being evaporated to the 60% of crude drug in whole weight；

6) mixed concentrated liquid is spray-dried, prepares compound for reducing blood suger dry powder；

7) in compound for reducing blood suger dry powder, adjuvant dextrin and food preservative (such as: potassium sorbate) are first added, Mix homogeneously, wherein compound for reducing blood suger dry powder is 10:100 with the weight proportion of dextrin；Compound for reducing blood suger dry powder It is 100:0.05 with the weight ratio of food preservative；It is added followed by appropriate distilled water, is placed in pellet processing machine System carries out pill, obtains little granular blood sugar reducing concentrated pill.

Prepared by embodiment 2 present composition granule

1) raw material (unit: × 10g) is weighed according to following proportioning

Cortex Lycii 20, Fructus Lycii 40, Fructus Ligustri Lucidi 40, Rhizoma Polygonati Odorati 15, Rhizoma Dioscoreae 10, Radix Ophiopogonis 15, Semen Cassiae 20

2) after medical material Cortex Lycii, Fructus Lycii, Fructus Ligustri Lucidi and Rhizoma Polygonati Odorati, Rhizoma Dioscoreae, Radix Ophiopogonis, Semen Cassiae being rinsed, Be placed in Multifunctional Chinese medicine and extract in still and mix homogeneously, add tap water and soak 2 hours, wherein, addition from The weight of water is 7:1 with the ratio of the weight (dry weight) of medicinal material mixture；

3) open multi-functional extraction still power supply to heat, be heated to 100 DEG C, keep constant temperature at this temperature to carry Take 2 hours, then filter, obtain extracting solution for the first time standby；

4) in filtering residue, add the weight (dry weight) of tap water, the weight of the tap water of addition and medicinal material mixture Ratio be 7:1, heating extraction, keep heating-up temperature be at 100 DEG C extract 1.5 hours, be filtrated to get second Secondary extracting solution；

5) carry out concentrating under reduced pressure in 2 extracting solution merging being placed on vacuum high energy evaporation concentrator, prepare mixed Closing concentrated solution, wherein, the relative pressure of concentrating under reduced pressure is that-0.05MPa (i.e. with an atmospheric pressure for 0, controls Pressure during concentrating under reduced pressure is-0.05MPa relative to an atmospheric pressure), it is heated to 100 DEG C, mixed concentrated liquid The weight ratio of weight and medicinal material mixture be 0.5:1, i.e. carry out being evaporated to the 50% of crude drug in whole weight；

6) mixed concentrated liquid is carried out lyophilization, prepare compound for reducing blood suger dry powder；

7) in compound for reducing blood suger dry powder, first add microcrystalline cellulose excipients and food preservative (the double Pyrusussuriensis of example Acid potassium), make granule after mix homogeneously, pack and i.e. obtain hypoglycemic granule, wherein compound for reducing blood suger dry powder and crystallite The weight proportion of cellulose is 10:100；Compound for reducing blood suger dry powder is 100 with the weight ratio of food preservative: 0.05。

Prepared by embodiment 3 present composition pill

1) raw material (unit: × 10g) is weighed according to following proportioning

Cortex Lycii 30, Fructus Lycii 25, Fructus Ligustri Lucidi 20, Rhizoma Polygonati Odorati 25, Rhizoma Dioscoreae 30, Radix Ophiopogonis 25, Semen Cassiae 15

2) after medical material Cortex Lycii, Fructus Lycii, Fructus Ligustri Lucidi and Rhizoma Polygonati Odorati, Rhizoma Dioscoreae, Radix Ophiopogonis, Semen Cassiae being rinsed, Be placed in Multifunctional Chinese medicine and extract in still and mix homogeneously, add tap water and soak 2 hours, wherein, addition from The weight of water is 8:1 with the ratio of the weight (dry weight) of medicinal material mixture；

3) open multi-functional extraction still power supply to heat, be heated to 100 DEG C, keep constant temperature at this temperature to carry Take 2 hours, then filter, obtain extracting solution for the first time standby；

4) in filtering residue, add the weight (dry weight) of tap water, the weight of the tap water of addition and medicinal material mixture Ratio be 5:1, heating extraction, keep heating-up temperature be at 100 DEG C extract 1.5 hours, be filtrated to get second Secondary extracting solution；

5) carry out concentrating under reduced pressure in 2 extracting solution merging being placed on vacuum high energy evaporation concentrator, prepare mixed Closing concentrated solution, wherein, the relative pressure of concentrating under reduced pressure is that-0.095MPa (i.e. with an atmospheric pressure for 0, is controlled Pressure during concentrating under reduced pressure processed is-0.095MPa relative to an atmospheric pressure), it is heated to 90 DEG C, mixing concentrates The weight of liquid and the weight ratio of medicinal material mixture are 0.6:1, i.e. carry out being evaporated to crude drug in whole weight 60%；

6) mixed concentrated liquid is spray-dried, prepares compound for reducing blood suger dry powder；

7) in compound for reducing blood suger dry powder, adjuvant dextrin and food preservative (such as potassium sorbate) are first added, mixed Closing uniformly, wherein compound for reducing blood suger dry powder is 10:100 with the weight proportion of dextrin；Compound for reducing blood suger dry powder with The weight ratio of food preservative is 100:0.05；It is added followed by appropriate distilled water, is placed in pill mechanism In carry out pill, obtain little granular blood sugar reducing concentrated pill.

Prepared by embodiment 4 present composition capsule

1) raw material (unit: × 10g) is weighed according to following proportioning

Cortex Lycii 10, Fructus Lycii 15, Fructus Ligustri Lucidi 50, Rhizoma Polygonati Odorati 35, Rhizoma Dioscoreae 35, Radix Ophiopogonis 10, Semen Cassiae 30

2) after medical material Cortex Lycii, Fructus Lycii, Fructus Ligustri Lucidi and Rhizoma Polygonati Odorati, Rhizoma Dioscoreae, Radix Ophiopogonis, Semen Cassiae being rinsed, Be placed in Multifunctional Chinese medicine and extract in still and mix homogeneously, add tap water and soak 1 hour, wherein, addition from The weight of water is 10:1 with the ratio of the weight (dry weight) of medicinal material mixture；

3) open multi-functional extraction still power supply to heat, be heated to 100 DEG C, keep constant temperature at this temperature to carry Take 3 hours, then filter, obtain extracting solution for the first time standby；

4) in filtering residue, add the weight (dry weight) of tap water, the weight of the tap water of addition and medicinal material mixture Ratio be 5:1, heating extraction, keep heating-up temperature be at 100 DEG C extract 3 hours, be filtrated to get second time Extracting solution；

5) carry out concentrating under reduced pressure in 2 extracting solution merging being placed on vacuum high energy evaporation concentrator, prepare mixed Closing concentrated solution, wherein, the relative pressure of concentrating under reduced pressure is that-0.07MPa (i.e. with an atmospheric pressure for 0, controls Pressure during concentrating under reduced pressure is-0.07MPa relative to an atmospheric pressure), it is heated to 90 DEG C, mixed concentrated liquid Weight is 0.4:1 with the weight ratio of medicinal material mixture, i.e. carries out being evaporated to the 40% of crude drug in whole weight；

6) mixed concentrated liquid is carried out lyophilization, prepare compound for reducing blood suger dry powder；

7) by compound for reducing blood suger dry powder and food preservative potassium sorbate, mix homogeneously, wherein compound for reducing blood suger Dry powder is 100:0.05 with the weight proportion of potassium sorbate；It is then charged into snap fit capsule, obtains Jiangtang capsule.

Prepared by embodiment 5 present composition pill

1) raw material (unit: × 10g) is weighed according to following proportioning

Cortex Lycii 35, Fructus Lycii 50, Fructus Ligustri Lucidi 10, Rhizoma Polygonati Odorati 10, Rhizoma Dioscoreae 10, Radix Ophiopogonis 30, Semen Cassiae 10

2) after medical material Cortex Lycii, Fructus Lycii, Fructus Ligustri Lucidi and Rhizoma Polygonati Odorati, Rhizoma Dioscoreae, Radix Ophiopogonis, Semen Cassiae being rinsed, Be placed in Multifunctional Chinese medicine and extract in still and mix homogeneously, add tap water and soak 3 hours, wherein, addition from The weight of water is 6:1 with the ratio of the weight (dry weight) of medicinal material mixture；

3) open multi-functional extraction still power supply to heat, be heated to 100 DEG C, keep constant temperature at this temperature to carry Take 1 hour, then filter, obtain extracting solution for the first time standby；

4) in filtering residue, add the weight (dry weight) of tap water, the weight of the tap water of addition and medicinal material mixture Ratio be 10:1, heating extraction, keep heating-up temperature be at 100 DEG C extract 1 hour, be filtrated to get second Secondary extracting solution；

5) carry out concentrating under reduced pressure in 2 extracting solution merging being placed on vacuum high energy evaporation concentrator, prepare mixed Closing concentrated solution, wherein, the relative pressure of concentrating under reduced pressure is that-0.07MPa (i.e. with an atmospheric pressure for 0, controls Pressure during concentrating under reduced pressure is-0.07MPa relative to an atmospheric pressure), it is heated to 90 DEG C, mixed concentrated liquid Weight is 0.7:1 with the weight ratio of medicinal material mixture, i.e. carries out being evaporated to the 70% of crude drug in whole weight；

6) mixed concentrated liquid is carried out lyophilization, prepare compound for reducing blood suger dry powder；

7) compound for reducing blood suger dry powder is mixed homogeneously with food preservative (such as potassium sorbate), wherein blood sugar lowering group Compound dry powder is 100:0.05 with the weight ratio of food preservative；It is added followed by appropriate distilled water, puts In pill mechanism, carry out pill, obtain little granular blood sugar reducing concentrated pill.

Claims (10)

1. there is the compositions reducing blood glucose function, it is characterized in that being made up of following raw material: Cortex Lycii, Fructus Lycii Son, Fructus Ligustri Lucidi, Rhizoma Polygonati Odorati, Rhizoma Dioscoreae, Radix Ophiopogonis, Semen Cassiae, wherein, the weight of described raw material is: ground Cortex Lycii 15-35, Fructus Lycii 15-50, Fructus Ligustri Lucidi 10-50, Rhizoma Polygonati Odorati 10-35, Rhizoma Dioscoreae 10-35,10-30 Radix Ophiopogonis, Semen Cassiae 10-30.

There is compositions the most as claimed in claim 1 that reduce blood glucose function, it is characterized in that the weight of described raw material Part proportioning is: Cortex Lycii 20-30, Fructus Lycii 25-40, Fructus Ligustri Lucidi 20-40, Rhizoma Polygonati Odorati 15-25, Rhizoma Dioscoreae 10-30, Radix Ophiopogonis 15-25, Semen Cassiae 15-20.

There is compositions the most as claimed in claim 1 that reduce blood glucose function, it is characterized in that the weight of described raw material Part proportioning is: Cortex Lycii 20, Fructus Lycii 30, Fructus Ligustri Lucidi 30, Rhizoma Polygonati Odorati 20, Rhizoma Dioscoreae 20, Radix Ophiopogonis 20, certainly Pine torch 20.

4. there is a preparation method for the compositions reducing blood glucose function, comprise the steps:

1) according to following weight preparation raw material:

Cortex Lycii 15-35, Fructus Lycii 15-50, Fructus Ligustri Lucidi 10-50, Rhizoma Polygonati Odorati 10-35, Rhizoma Dioscoreae 10-35, Radix Ophiopogonis 10-30, Semen Cassiae 10-30；

2) by after described crude drug mix homogeneously, mixing crude drug is carried out heating extraction, wherein said heating Extract and include the step that order below is carried out:

A) feeding the mixture into water soaking, the weight of the water of addition and the weight ratio of mixture are 6-10: 1, carry out heating extraction for the first time after soaking 1-3 hour, heating-up temperature is 100 DEG C, keeping temperature is Extract 1-3 hour under conditions of 100 DEG C；

B) first time extracting solution is filtered, collect filtrate；

C) in filtering residue add water, carry out second time heating extraction, wherein, the weight of the water of addition with The weight ratio of mixture is 5-10:1, and heating-up temperature is 100 DEG C, at the bar keeping temperature to be 100 DEG C Extract 1-3 hour under part；

D) filter, the filtrate of twice filtration is merged and obtains extracting solution；

3) extracting solution collected is carried out concentration, prepare concentrated solution；

4) concentrated solution is dried process, to obtain final product.

5. preparation method as claimed in claim 4, is characterized in that, step A) described in the heating extraction time 1.5-2.5 hour, the weight of the water of addition and the weight ratio of mixture were preferably 7-8:1；Step C) in add The weight of the water entered and the weight ratio of mixture are 5-7:1.

6. the preparation method as described in claim 4 or 5, is characterized in that, step 3) described in extracting solution dense Controlling temperature in contracting processing procedure and be 80-100 DEG C, relative pressure is less than-0.05MPa.

7. the preparation method as described in claim 4 or 5, is characterized in that, step 3) described in concentrated solution The gross weight of weight and crude drug Cortex Lycii, Fructus Lycii, Fructus Ligustri Lucidi, Rhizoma Polygonati Odorati, Rhizoma Dioscoreae, Radix Ophiopogonis and Semen Cassiae it Ratio is 0.4-0.7:1.

8. the preparation method as described in claim 4 or 5, is characterized in that the compositions also included after dried The pharmaceutically acceptable adjuvant of middle addition, mixes.

9. there is a preparation method for the compositions reducing blood glucose function, comprise the steps:

1) according to following weight preparation raw material:

Cortex Lycii 20-30, Fructus Lycii 25-40, Fructus Ligustri Lucidi 20-40, Rhizoma Polygonati Odorati 15-25, Rhizoma Dioscoreae 10-30, Radix Ophiopogonis 15-25, Semen Cassiae 15-20；

2) by after described crude drug mix homogeneously, mixing crude drug is carried out heating extraction, wherein said heating Extract and include the step that order below is carried out:

A) feeding the mixture into water soaking, the weight of the water of addition and the weight ratio of mixture are 6-10: 1, carry out heating extraction for the first time after soaking 1-3 hour, heating-up temperature is 100 DEG C, keeping temperature is Extract 1-3 hour under conditions of 100 DEG C；

B) first time extracting solution is filtered, collect filtrate；

C) in filtering residue add water, carry out second time heating extraction, wherein, the weight of the water of addition with The weight ratio of mixture is 5-10:1, and heating-up temperature is 100 DEG C, at the bar keeping temperature to be 100 DEG C Extract 1-3 hour under part；

D) filter, the filtrate of twice filtration is merged and obtains extracting solution；

3) extracting solution collected is carried out concentration, prepare concentrated solution；

4) concentrated solution is dried process, to obtain final product.

10. there is a preparation method for the compositions reducing blood glucose function, comprise the steps:

1) according to following weight preparation raw material:

Cortex Lycii 20, Fructus Lycii 30, Fructus Ligustri Lucidi 30, Rhizoma Polygonati Odorati 20, Rhizoma Dioscoreae 20, Radix Ophiopogonis 20, Semen Cassiae 20；

2) by after described crude drug mix homogeneously, mixing crude drug is carried out heating extraction, wherein said heating Extract and include the step that order below is carried out:

A) feeding the mixture into water soaking, the weight of the water of addition and the weight ratio of mixture are 6-10: 1, carry out heating extraction for the first time after soaking 1-3 hour, heating-up temperature is 100 DEG C, keeping temperature is Extract 1-3 hour under conditions of 100 DEG C；

B) first time extracting solution is filtered, collect filtrate；

C) in filtering residue add water, carry out second time heating extraction, wherein, the weight of the water of addition with The weight ratio of mixture is 5-10:1, and heating-up temperature is 100 DEG C, at the bar keeping temperature to be 100 DEG C Extract 1-3 hour under part；

D) filter, the filtrate of twice filtration is merged and obtains extracting solution；

3) extracting solution collected is carried out concentration, prepare concentrated solution；

4) concentrated solution is dried process, to obtain final product.