CN103776662A - Collecting device and method of root exudates from xerophyte - Google Patents
Collecting device and method of root exudates from xerophyte Download PDFInfo
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- CN103776662A CN103776662A CN201410029083.7A CN201410029083A CN103776662A CN 103776662 A CN103776662 A CN 103776662A CN 201410029083 A CN201410029083 A CN 201410029083A CN 103776662 A CN103776662 A CN 103776662A
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Abstract
The invention provides a collecting device of root exudates from xerophytes. The collecting device comprises a cultivating bucket, wherein the bottom surface of the cultivating bucket is communicated with a rubber tube, and the other end of the rubber tube is connected with an injector, a soil vermiculite mixed culture medium layer and a filter layer are sequentially spread in the cultivating bucket from top to bottom, and an irrigation tube is further arranged in the cultivating bucket. The invention also provides a collecting method for the root exudates from xerophytes, which is designed according to the collecting device. The collecting device and method are suitable for research on plants under soil cultivation condition, especially the root exudates from xerophytes; compared with water-culture or simple medium cultivation method, the method provided by the invention has the advantages that the actual condition of the root exudates from xerophytes under the natural condition can be better reflected; meanwhile, the collecting device cannot disturb the root growth or damage the rhizoapple environment in the premise of guaranteeing the normal growth of the plant, and the root damage and bleeding disturbance caused by collecting the root exudates through a traditional earth culture method are avoided.
Description
Technical field
The present invention relates to a kind of xerophyte root exudates gathering-device and collection method thereof.
Background technology
At present, the collection of root exudates mainly contains water culture, substrate culture collecting method, earth culture method.Water culture does not need special gathering-device, but the general roots development of hydroponic plant is less, there is no mechanical resistance, has larger difference with ventilation situation, Nutrient distribution, the root system branched structure of growing under real earth culture condition.Therefore water culture cannot be obtained xerophytic root exudates.Matrix cultivation can guarantee the ventilation of root system, also has certain mechanical resistance, but has larger difference with the root exudates under state of nature.
Tradition earth culture method is collected root exudates and is mainly adopted two kinds of methods, and the one, after earth culture plant growth a period of time, its root system is taken out, with distilling the lower rhizosphere soil of washing, centrifugal or filtration after concussion, gained is root exudates.The 2nd, the plant that grows into certain phase is taken out from soil, its root system surface soil layer to be rinsed well, then used distilled water drip washing root system, leacheate is as root exudates.In whole operating process, root system very easily sustains damage, and can comprise inclusions and the bleeding sap of a lot of root systems itself in the solution of collecting, and this method be generally only applicable to small-sized plant or seedling stage plant.Except above several method, according to the difference of research purpose and research object, some researcher's designed, designeds special device collect root exudates.As continuous root exudates collection system (Tang and Young, 1982), this device utilizes macroporous adsorbing resin for purification root exudates, and realize organic enrichment and separated, but the more difficult control of the flow of nutrient solution.Recently, the root case that Oburger etc. (2013) has designed a set of novelty is collected the root exudates of earth culture maize seedling.This device can neither destroy the self-sow situation between root system and soil, also selection site preferably, but cannot collect the root exudates of piece root or tuberous plant.
In sum, root exudates gathering-device used is all not suitable for the collection of xerophyte root exudates at present, and this has limited the research to xerophyte root exudates greatly.The present invention proposes a kind of gathering-device and method of xerophyte root exudates, be expected to become the powerful of xerophyte root exudates research.
Summary of the invention
The present invention improves the problems referred to above, and the technical problem to be solved in the present invention is the collection that existing root exudates gathering-device is all not suitable for xerophyte root exudates, has limited the research to xerophyte root exudates.
Specific embodiment of the invention scheme is: a kind of xerophyte root exudates gathering-device, comprise a cultivation bucket, described cultivation bucket bottom is funnel-form, described cultivation bucket bottom surface is communicated with a rubber tube, the described rubber tube other end is connected with syringe, in described cultivation bucket, be equipped with successively soil vermiculite Mixed culture matrix layer and filtering layer from top to bottom, described cultivation in bucket is laid with the irrigation pipe that inserts soil vermiculite Mixed culture matrix layer, and Mixed culture matrix layer upper surface exposed in described irrigation pipe upper end.
Further, described irrigation pipe is pvc pipe, and pvc pipe carries out one-sided boring, and seal with absorbent cotton lower end, and pipe shaft is enclosed with 2 layers of gauze, and while burying, boring is surveyed towards cultivating bucket center, four pvc pipe journey square symmetry, and matrix 3～4 ㎝ are exposed in upper end.
Further, described filtering layer comprises sandy layer and is positioned at the nonwoven layer below sandy layer.
Further, described rubber tube middle part has liquid stopping clamp.
A kind of xerophyte root exudates collection method, comprises a kind of xerophyte root exudates gathering-device as claimed in claim 4, comprises the following steps:
(1) first make root exudates gathering-device, filling soil vermiculite Mixed culture matrix and filtering layer in cultivation bucket;
(2) bury irrigation pipe, and planting plants;
(3) in plant cultivation process, pass through the regular irrigation soils leaching liquor of irrigation pipe, can guarantee the normal growth of plant;
(4) collect before root exudates, the leaching liquor that slowly pours into source identical with culture matrix soil from irrigation pipe, to till there are flowing out in rubber tube, is drained the solution of outflow gently with syringe, extract solution out and be root exudates;
(5) root exudates of step (4) being collected and through 0.22 μ m membrane filtration, and by the secretion after filtering transfer under 4 ℃ of conditions, preserve stand-by.
Further, described leaching liquor is by pressing the water and soil mass ratio of 2:1 with the soil in the identical source of culture matrix, shakes 4 hours and spend the night after leaving standstill to make.
Further, described culture matrix is the potpourri of soil and vermiculite, and volume ratio is 1:0.5 ~ 1:1.5, and filtering layer is made up of the sand of cleaning on nonwoven fabrics and laying and the nonwoven fabrics of oven dry, and sand is 0.5 ~ 3kg, particle diameter 0.5 ~ 1mm.Nonwoven fabrics filling thickness is 3 ~ 8cm, guarantees that root exudates is collected liquid limpid.
Further, described machine glass tank, organic glass bucket inwall is frosted structure, and ladle body is cylindric, and bucket shoulder and bucket stem are upside down funnel shape, bucket shoulder height 3 ~ 8cm, bucket stem 3 ~ 8cm, diameter 2 ~ 4 cm, bucket stem end closes up and is convenient to the installation of rubber tube.
Compared with prior art, the present invention has following beneficial effect:
(1) the present invention is applicable to the research of plant under soil cultivation condition (especially xerophyte) root exudates.Cultivate and compare with simple matrix with water planting, more can reflect the actual state of secretions from plant roots under natural conditions.
(2) the present invention adopts the potpourri of soil and vermiculite as culture matrix, has both guaranteed the nutritional need of plant normal growth, is conducive to again ooze under moisture, can guarantee effective collection of root exudates, close to state of nature.Difference needs the collection of water type secretions from plant roots to realize by the ratio of adjusting soil and vermiculite.
(3) the present invention is guaranteeing under the prerequisite of plant normal growth, not disturbance root growth, does not destroy rhizospheric environment, has avoided the root system damage and the bleeding that while collecting root exudates because of traditional earth culture method, have caused to disturb.
(4) utilization of the present invention is irrigated through the pvc pipe of special processing, guarantees that moisture slowly permeates to plant root from pipe, and this makes the collection of root exudates more efficient.Secondly the gas penetration potential of plant root has been guaranteed in the use of pvc pipe.
(5) the present invention adopts clean fine sand and nonwoven fabrics as filtration unit, has avoided the loss of soil and vermiculite, is conducive to the collection of percolate.
(6) the present invention utilizes syringe to carry out the collection of root exudates, can need to extract accurately root exudates according to experiment, compared with peristaltic pump in prior device, extracting dynamics more easy to control, can spoiled soil structure, the fluid sample simultaneously obtaining can directly be crossed film and processes and analyze for HPLC.
(7) the present invention's solution of filling with in the time that root exudates is collected is soil extraction rather than tap water, has avoided like this collecting because of root exudates the soil nutrient loss causing, and makes root exudates collection procedure drop to minimum on the impact of plant growth.
Accompanying drawing explanation
Fig. 1 is xerophyte root exudates gathering-device structural representation of the present invention.
Embodiment
Below in conjunction with the drawings and specific embodiments, the present invention will be further described in detail.
As shown in Figure 1, culture apparatus is the organic glass bucket 2 of inwall frosted, and filling culture matrix is the potpourri 3 of soil and vermiculite, and research object is for treating measuring plants 1.Be the pvc pipe 4 through special processing for the device of irrigating, fill and eluriate clean sand 5 in organic glass bucket, nonwoven fabrics 6 is filled at bucket stem place.Control the collection of root exudates with agrafe 7, rubber tube 8 other ends are connected with gathering-device and syringe 9 for extracting.
The collection of glutinous rehmannia root exudates:
First customize high 40 ㎝, thick 0.2 ㎝, ladle body 28 ㎝, 21 centimetres of diameters, bucket shoulder 4 ㎝, the bottomless inner frosted organic glass bucket of bucket stem 8 diameter 4 ㎝, fills nonwoven fabrics and sand, and nonwoven fabrics is filled in barrel stem, thickness 5 ㎝.Sand covers on nonwoven fabrics, sand 2 ㎏, particle diameter 1 ㎜.Then load culture matrix, soil vermiculite volume ratio is 1:1, fills up culture vessel, and soil is taken from glutinous rehmannia planting base, Wen County, Henan.When filling, be square symmetry and bury pvc pipe, pipe range 25 ㎝, diameter 2.5 ㎝, seal with absorbent cotton lower end, 2 layers of gauze parcels for pipe shaft, matrix 3 ㎝ are exposed in upper end, are convenient to irrigate.At culture matrix center plantation glutinous rehmannia reed head, from 4, irrigated 500 ml soil extractions every 10 days, guarantee the normal growth of glutinous rehmannia, now agrafe 7 is closed.According to experimental design, expand to expand in early stage, piece root at glutinous rehmannia seedling stage, piece root elongating stage, piece root and expand later stage and Jiaozhuo, irrigation soils leaching liquor effluent harvest time south soil in mid-term, piece root by the water and soil mass ratio of 2:1, shaking within 4 hours, spend the night leave standstill after make 1000 ml, open agrafe 7, now there is solution to flow out from rubber tube, drain gently with syringe 9, gained solution is root exudates.The timely mistake 0.22 μ m miillpore filter of root exudates of collecting, and be transferred under 4 ℃ of conditions and store, adopt HPLC to analyze its coumaric acid, P-hydroxybenzoic acid, vanillic acid, syringic acid and ferulaic acid content.
The foregoing is only preferred embodiment of the present invention, all equalizations of doing according to the present patent application the scope of the claims change and modify, and all should belong to covering scope of the present invention.
Claims (8)
1. an xerophyte root exudates gathering-device, it is characterized in that, comprise a cultivation bucket, described cultivation bucket bottom is funnel-form, described cultivation bucket bottom surface is communicated with a rubber tube, and the described rubber tube other end is connected with syringe, is equipped with successively soil vermiculite Mixed culture matrix layer and filtering layer in described cultivation bucket from top to bottom, described cultivation in bucket is laid with the irrigation pipe that inserts soil vermiculite Mixed culture matrix layer, and Mixed culture matrix layer upper surface exposed in described irrigation pipe upper end.
2. a kind of xerophyte root exudates gathering-device according to claim 1, described irrigation pipe is pvc pipe, and seal with absorbent cotton pvc pipe lower end, and pipe shaft is enclosed with 2 layers of gauze, and matrix 3～4 ㎝ are exposed in irrigation pipe upper end.
3. a kind of xerophyte root exudates gathering-device according to claim 1, described filtering layer comprises sandy layer and is positioned at the nonwoven layer of sandy layer below.
4. a kind of xerophyte root exudates gathering-device according to claim 2, described rubber tube middle part has liquid stopping clamp.
5. an xerophyte root exudates collection method, comprises a kind of xerophyte root exudates gathering-device as claimed in claim 4, it is characterized in that, comprises the following steps:
(1) first make root exudates gathering-device, filling soil vermiculite Mixed culture matrix and filtering layer in cultivation bucket;
(2) bury irrigation pipe, and planting plants;
(3) in plant cultivation process, pass through the regular irrigation soils leaching liquor of irrigation pipe, can guarantee the normal growth of plant;
(4) collect before root exudates, the leaching liquor that slowly pours into source identical with culture matrix soil from irrigation pipe, to till there are flowing out in rubber tube, is drained the solution of outflow gently with syringe, extract solution out and be root exudates;
(5) root exudates of step (4) being collected and through 0.22 μ m membrane filtration, and by the secretion after filtering transfer under 4 ℃ of conditions, preserve stand-by.
6. a kind of xerophyte root exudates collection method according to claim 5, described leaching liquor is by pressing the water and soil mass ratio of 2:1 with the soil in the identical source of culture matrix, shakes 4 hours and spend the night after leaving standstill to make.
7. a kind of xerophyte root exudates collection method according to claim 5, described culture matrix is the potpourri of soil and vermiculite, volume ratio is 1:0.5～1:1.5, filtering layer is made up of the sand of cleaning on nonwoven fabrics and laying and the nonwoven fabrics of oven dry, sand is 0.5～3kg, particle diameter 0.5～1m, described nonwoven fabrics filling thickness is 3～8cm, guarantees that root exudates is collected liquid limpid.
8. a kind of xerophyte root exudates collection method according to claim 5, described machine glass tank, organic glass bucket inwall is frosted structure, ladle body is cylindric, bucket shoulder and bucket stem are upside down funnel shape, bucket shoulder height 3～8cm, bucket stem 3～8cm, diameter 2～4 cm, bucket stem end closes up and is convenient to the installation of rubber tube.
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| CN105973632A (en) * | 2016-05-05 | 2016-09-28 | 中国农业科学院烟草研究所 | Plant root secreta generation and collection device and collection method thereof |
| CN107036873A (en) * | 2017-05-09 | 2017-08-11 | 黑龙江大学 | A kind of device and method of in-situ extraction arbuscular mycorrhiza secretions from plant roots |
| CN109247163A (en) * | 2018-09-29 | 2019-01-22 | 浙江农林大学 | It is a kind of to divide root device for what is studied soil nutrient heterogeneity rhizosphere respondent behavior |
| CN110672351A (en) * | 2019-11-08 | 2020-01-10 | 华南农业大学 | Device and method for collecting root exudates of gramineous plants |
| CN111699960A (en) * | 2020-06-03 | 2020-09-25 | 长治学院 | Traditional Chinese medicine material planting device and method |
| CN112649241A (en) * | 2020-12-16 | 2021-04-13 | 山西农业大学 | Adsorption type root system secretion collecting system |
| CN113049300A (en) * | 2021-02-09 | 2021-06-29 | 湖南农业大学 | Separation and collection device and method for plant root exudates |
| CN113218700A (en) * | 2021-04-29 | 2021-08-06 | 河海大学 | Membrane collection device and method for plant root exudates in soil culture mode |
| CN113514269A (en) * | 2021-06-03 | 2021-10-19 | 海南大学 | Root exudate normal position collection system |
| CN113804499A (en) * | 2021-08-26 | 2021-12-17 | 贵州大学 | Pinus massoniana rhizosphere secretion collecting device and collecting method thereof |
| CN114112480A (en) * | 2021-12-17 | 2022-03-01 | 贵州省生物研究所 | Device and method for collecting pepper rhizosphere secretion |
| CN114532204A (en) * | 2022-02-28 | 2022-05-27 | 安顺市宝林科技中药饮片有限公司 | Planting device and planting method for rhizome traditional Chinese medicine materials |
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| CN104749000A (en) * | 2015-04-02 | 2015-07-01 | 上海交通大学 | Device and method for extracting helophyte root exudates |
| CN105973632A (en) * | 2016-05-05 | 2016-09-28 | 中国农业科学院烟草研究所 | Plant root secreta generation and collection device and collection method thereof |
| CN105973632B (en) * | 2016-05-05 | 2019-01-25 | 中国农业科学院烟草研究所 | Secretions from plant roots generation and collection device and its collection method |
| CN107036873A (en) * | 2017-05-09 | 2017-08-11 | 黑龙江大学 | A kind of device and method of in-situ extraction arbuscular mycorrhiza secretions from plant roots |
| CN107036873B (en) * | 2017-05-09 | 2023-07-04 | 黑龙江大学 | Device and method for in-situ extraction of arbuscular mycorrhizal plant root secretions |
| CN109247163A (en) * | 2018-09-29 | 2019-01-22 | 浙江农林大学 | It is a kind of to divide root device for what is studied soil nutrient heterogeneity rhizosphere respondent behavior |
| CN110672351A (en) * | 2019-11-08 | 2020-01-10 | 华南农业大学 | Device and method for collecting root exudates of gramineous plants |
| CN110672351B (en) * | 2019-11-08 | 2021-02-02 | 华南农业大学 | Device and method for collecting root exudates of gramineous plants |
| CN111699960A (en) * | 2020-06-03 | 2020-09-25 | 长治学院 | Traditional Chinese medicine material planting device and method |
| CN111699960B (en) * | 2020-06-03 | 2022-02-15 | 长治学院 | Traditional Chinese medicine material planting device and method |
| CN112649241A (en) * | 2020-12-16 | 2021-04-13 | 山西农业大学 | Adsorption type root system secretion collecting system |
| CN112649241B (en) * | 2020-12-16 | 2023-05-26 | 山西农业大学 | Adsorption type root system secretion collecting system |
| CN113049300A (en) * | 2021-02-09 | 2021-06-29 | 湖南农业大学 | Separation and collection device and method for plant root exudates |
| CN113049300B (en) * | 2021-02-09 | 2022-03-11 | 湖南农业大学 | Separation and collection device and method for plant root exudates |
| CN113218700A (en) * | 2021-04-29 | 2021-08-06 | 河海大学 | Membrane collection device and method for plant root exudates in soil culture mode |
| CN113218700B (en) * | 2021-04-29 | 2022-04-15 | 河海大学 | Membrane collection device and method for plant root exudates in soil culture mode |
| CN113514269B (en) * | 2021-06-03 | 2022-03-04 | 海南大学 | Root exudate normal position collection system |
| CN113514269A (en) * | 2021-06-03 | 2021-10-19 | 海南大学 | Root exudate normal position collection system |
| CN113804499A (en) * | 2021-08-26 | 2021-12-17 | 贵州大学 | Pinus massoniana rhizosphere secretion collecting device and collecting method thereof |
| CN114112480A (en) * | 2021-12-17 | 2022-03-01 | 贵州省生物研究所 | Device and method for collecting pepper rhizosphere secretion |
| CN114532204A (en) * | 2022-02-28 | 2022-05-27 | 安顺市宝林科技中药饮片有限公司 | Planting device and planting method for rhizome traditional Chinese medicine materials |
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