CN101598638A - A kind of device for collecting root exudate in situ - Google Patents
A kind of device for collecting root exudate in situ Download PDFInfo
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- CN101598638A CN101598638A CNA2009100121306A CN200910012130A CN101598638A CN 101598638 A CN101598638 A CN 101598638A CN A2009100121306 A CNA2009100121306 A CN A2009100121306A CN 200910012130 A CN200910012130 A CN 200910012130A CN 101598638 A CN101598638 A CN 101598638A
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Abstract
A kind of device for collecting root exudate in situ, comprise incubator, support, illumination cultivation light, straight shape glass tube, peristaltic pump, nutrition bottle, resin column, surge flask and receiving flask, culture matrix is housed in the incubator, incubator covers plastic sheeting outward, seal pad is established in the incubator upper end, and a thermometer is assigned in the incubator side; There is a through hole incubator lower end, and this through hole seals with rubber stopper, places glass wool above the rubber stopper; Incubator fixes with support, and this support is installed on the base; Support top is equipped with adjustable support, and illumination cultivation light is equipped with in the upper end of this adjustable support; One porous ring glass pipe is arranged in the culture matrix in the incubator, connect shape glass tube always under this porous ring glass pipe, this straight shape glass tube is passed by the bottom rubber stopper, is inserted in the nutrition bottle by peristaltic pump; Have a resin column to collect glass tube, its upper end is passed rubber stopper and is extend in the interior glass wool of incubator, and its other end is connected with resin column; The resin column lower end connects a glass tube, and the other end of this glass tube is inserted in the surge flask; Receiving flask is connected with surge flask by collection tube.The present invention can truly reflect the feature of root exudates when plant grows in soil environment in the laboratory.
Description
Technical field:
The present invention relates to heavy-metal contaminated soil phytoremediation research device, specifically a kind of under laboratory condition original position collect the device of secretions from plant roots under the heavy metal stress condition continuously.
Background technology:
At present along with the development of heavy-metal contaminated soil phytoremediation technology, the research of secretions from plant roots is received much attention the feature of its component and change and directly influence the growth of plant and the bioavailability of heavy metal in soil.
Specified plant root exudates feasibility in field test is little under the collection heavy metal pollution condition.Moisture in the soil, temperature, nutrient, oxygen content, potential of hydrogen etc. change irregular, and the growth according to specific time and site of plant differs greatly.In addition, the existence of a large amount of microorganisms will certainly influence the growth of root system of plant and the metamorphosis of heavy metal in the soil, therefore, the root exudates that original position is collected under the natural conditions often can not accurately reflect the feature of the root exudates of plant itself, and then has influenced the corresponding relation between research secretions from plant roots and the heavy metal toxicity.
Now, root exudates is to utilize the method for Liquid Culture to collect in the laboratory mostly.At first, can effectively regulate and control such as temperature in the laboratory, conditions such as humidity reach the top condition that plant grows, and secondly are that the Liquid Culture collection method is fairly simple, and the time of collecting is short, disturbs less relatively.But, Liquid Culture is different from soil environment, there is not the extruding problem in the soil environment in root system of plant in liquid, simultaneously, oxygen content in the soil also affects the plant root exudation process greatly, therefore, Liquid Culture is collected root exudates can not truly reflect root exudates when plant grows in soil environment feature.
Summary of the invention
The purpose of this invention is to provide a kind of device for collecting root exudate in situ that can in the laboratory, collect secretions from plant roots in the true soil.
The present invention is achieved through the following technical solutions:
A kind of device for collecting root exudate in situ comprises incubator, support, illumination cultivation light, straight shape glass tube, peristaltic pump, feeding solution, resin column, surge flask and receiving flask.Culture matrix is housed in the incubator, and incubator covers plastic sheeting outward, and seal pad is established in the incubator upper end, and a thermometer is assigned in the incubator side.There is a through hole incubator lower end, and this through hole seals with rubber stopper, places glass wool above the rubber stopper.Incubator fixes with support, and this support is installed on the base.Support top is equipped with adjustable support, and illumination cultivation light is equipped with in the upper end of this adjustable support.One porous ring glass pipe is arranged in the culture matrix in the incubator, connect shape glass tube always under this porous ring glass pipe, this straight shape glass tube is passed by the bottom rubber stopper, is inserted in the feeding solution by peristaltic pump.Have a resin column to collect glass tube, its upper end is passed rubber stopper and is extend in the interior glass wool of incubator, and its other end is connected with resin column.The resin column lower end connects a glass tube, and the other end of this glass tube is inserted in the surge flask.Receiving flask is connected with surge flask by collection tube.
When adopting this original position root exudates gathering-device, culture matrix (silica sand) through nitric acid dousing 1 day, is cleaned with clear water, in the incubator of packing into behind the autoclaving.Under the research pollution condition, root exudates the time, can in culture matrix, add the variable concentrations heavy metal.One deck glass wool is filled up in the incubator bottom, and seals with rubber stopper.Before beginning to collect secretion, plant seedlings to be transplanted in the incubator culture matrix, incubator top seals with seal pad.Open the illumination cultivation light simulating nature photoperiod (10 hours/day) in the incubation, utilize peristaltic pump that the nutrient solution in the feeding solution is arrived porous ring glass pipe through straight shape glass tube simultaneously root system of plant is supplemented the nutrients and moisture.The culture period interocclusal record soil moisture.When collecting root exudates, nutrient solution in the incubator is drained, in incubator, replenish the deionization sterilized water, wash 3 times, plant was cultivated 1 day in the deionization sterilized water, utilized resin column collection glass tube, resin column, surge flask and the collection tube of incubator bottom to collect afterwards.
This original position root exudates gathering-device is at experiment lab simulation soil physics attribute, create plant rhizosphere zone aseptic condition, avoid microorganism to the influence of plant root growth and growth of microorganism metabolic product as much as possible to the root exudates component, utilize root system of plant directly to supplement the nutrients simultaneously, and root system directly washes the method for root exudates, not only can supply with nutrition for root to greatest extent, can also reach the purpose of a large amount of collection real plants root exudates.
Description of drawings:
Fig. 1 is a structural representation of the present invention;
The label of each parts among the figure is: 1 for receiving flask, 2 for collection tube, 3 for surge flask, 4 for resin column, 5 for resin column collect glass tube, 6 for incubator, 7 for support, 8 for thermometer, 9 for illumination cultivation light, 10 for adjustable support, 11 for seal pad, 12 for porous ring glass pipe, 13 for culture matrix, 14 for glass wool, 15 for rubber stopper, 16 for straight shape glass tube, 17 for peristaltic pump, 18 be feeding solution.
Embodiment
See Fig. 1, a kind of device for collecting root exudate in situ comprises incubator 6, support 7, illumination cultivation light 9, straight shape glass tube 16, peristaltic pump 17, feeding solution 18, resin column 4, surge flask 2 and receiving flask 1.Culture matrix 13 is housed in the incubator 6, and the culture matrix 13 in this example covers plastic sheeting for sterilization silica sand outside the incubator 6, and plastic sheeting can keep plant root lucifuge state, and seal pad 11 is established in incubator 6 upper ends, and a thermometer 8 is assigned in incubator 6 sides.There is a through hole incubator 6 lower ends, and this through hole is placed glass wool 14 with rubber stopper 15 sealings above the rubber stopper 15.Incubator 6 usefulness supports 7 fix, and this support 7 is installed on the base.Support 7 tops are equipped with adjustable support 10, and illumination cultivation light 9 is equipped with in the upper end of this adjustable support 10.One porous ring glass pipe 12 is arranged in the culture matrix 13 in the incubator 6, and this porous ring glass pipe connects shape glass tube 16 always for 12 times, and this straight shape glass tube 16 is passed by bottom rubber stopper 15, is inserted in the feeding solution 18 by peristaltic pump 17.Have a resin column to collect glass tube 4, its upper end is passed rubber stopper 15 and is extend in the glass wool 14 in the incubator 6, and its other end is connected with resin column 4.Resin column 4 lower ends connect a glass tube, and the other end of this glass tube is inserted in the surge flask 2.Receiving flask 1 is connected with surge flask 2 by collection tube 3.
Claims (1)
1, a kind of device for collecting root exudate in situ, comprise incubator, support, illumination cultivation light, straight shape glass tube, peristaltic pump, feeding solution, resin column, surge flask and receiving flask, it is characterized in that: culture matrix is housed in the incubator, incubator covers plastic sheeting outward, seal pad is established in the incubator upper end, and a thermometer is assigned in the incubator side; There is a through hole incubator lower end, and this through hole seals with rubber stopper, places glass wool above the rubber stopper; Incubator fixes with support, and this support is installed on the base; Support top is equipped with adjustable support, and illumination cultivation light is equipped with in the upper end of this adjustable support; One porous ring glass pipe is arranged in the culture matrix in the incubator, connect shape glass tube always under this porous ring glass pipe, this straight shape glass tube is passed by the bottom rubber stopper, is inserted in the feeding solution by peristaltic pump; Have a resin column to collect glass tube, its upper end is passed rubber stopper and is extend in the interior glass wool of incubator, and its other end is connected with resin column; The resin column lower end connects a glass tube, and the other end of this glass tube is inserted in the surge flask; Receiving flask is connected with surge flask by collection tube.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009100121306A CN101598638B (en) | 2009-06-19 | 2009-06-19 | Device for collecting root exudate in situ |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN2009100121306A CN101598638B (en) | 2009-06-19 | 2009-06-19 | Device for collecting root exudate in situ |
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| CN101598638A true CN101598638A (en) | 2009-12-09 |
| CN101598638B CN101598638B (en) | 2011-09-14 |
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| CN2009100121306A Expired - Fee Related CN101598638B (en) | 2009-06-19 | 2009-06-19 | Device for collecting root exudate in situ |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103776662A (en) * | 2014-01-22 | 2014-05-07 | 福建农林大学 | Collecting device and method of root exudates from xerophyte |
| CN105973632A (en) * | 2016-05-05 | 2016-09-28 | 中国农业科学院烟草研究所 | Plant root secreta generation and collection device and collection method thereof |
| CN106053135A (en) * | 2016-05-10 | 2016-10-26 | 中国科学院成都生物研究所 | A grassland herbal plant root exudate in-situ collecting method |
| CN107976330A (en) * | 2017-11-21 | 2018-05-01 | 东北师范大学 | The collection device in situ and method of secretion from root system of plant in pot and rhizosphere soil |
| CN112539969A (en) * | 2020-11-30 | 2021-03-23 | 赣州久创科技有限公司 | Continuous collection device of plant root system secretion |
| CN112997731A (en) * | 2021-02-19 | 2021-06-22 | 农业农村部南京农业机械化研究所 | Plant root absorption data collection device and method |
| CN113049300A (en) * | 2021-02-09 | 2021-06-29 | 湖南农业大学 | Separation and collection device and method for plant root exudates |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2859501Y (en) * | 2005-06-20 | 2007-01-17 | 华南农业大学 | Excretion collecting device for root system of plant |
-
2009
- 2009-06-19 CN CN2009100121306A patent/CN101598638B/en not_active Expired - Fee Related
Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103776662A (en) * | 2014-01-22 | 2014-05-07 | 福建农林大学 | Collecting device and method of root exudates from xerophyte |
| CN103776662B (en) * | 2014-01-22 | 2016-01-27 | 福建农林大学 | A kind of xerophyte root exudates gathering-device and collection method thereof |
| CN105973632A (en) * | 2016-05-05 | 2016-09-28 | 中国农业科学院烟草研究所 | Plant root secreta generation and collection device and collection method thereof |
| CN105973632B (en) * | 2016-05-05 | 2019-01-25 | 中国农业科学院烟草研究所 | Secretions from plant roots generation and collection device and its collection method |
| CN106053135A (en) * | 2016-05-10 | 2016-10-26 | 中国科学院成都生物研究所 | A grassland herbal plant root exudate in-situ collecting method |
| CN107976330A (en) * | 2017-11-21 | 2018-05-01 | 东北师范大学 | The collection device in situ and method of secretion from root system of plant in pot and rhizosphere soil |
| CN112539969A (en) * | 2020-11-30 | 2021-03-23 | 赣州久创科技有限公司 | Continuous collection device of plant root system secretion |
| CN112539969B (en) * | 2020-11-30 | 2023-11-14 | 赣州久创科技有限公司 | Continuous collection device of plant root secretion |
| CN113049300A (en) * | 2021-02-09 | 2021-06-29 | 湖南农业大学 | Separation and collection device and method for plant root exudates |
| CN113049300B (en) * | 2021-02-09 | 2022-03-11 | 湖南农业大学 | Separation and collection device and method for plant root exudates |
| CN112997731A (en) * | 2021-02-19 | 2021-06-22 | 农业农村部南京农业机械化研究所 | Plant root absorption data collection device and method |
| CN112997731B (en) * | 2021-02-19 | 2022-06-28 | 农业农村部南京农业机械化研究所 | Plant root absorption data collection device and method |
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| Publication number | Publication date |
|---|---|
| CN101598638B (en) | 2011-09-14 |
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