CN106153390A - A kind of movable-type secretions from plant roots classified collection device and collection method - Google Patents
A kind of movable-type secretions from plant roots classified collection device and collection method Download PDFInfo
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- CN106153390A CN106153390A CN201510158373.6A CN201510158373A CN106153390A CN 106153390 A CN106153390 A CN 106153390A CN 201510158373 A CN201510158373 A CN 201510158373A CN 106153390 A CN106153390 A CN 106153390A
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Abstract
The invention discloses a kind of movable-type secretions from plant roots classified collection device and collection method.Device includes a plant culturing bucket, is filled with fine sand filter course, solid culture substrate layer, block of glass wool and haydite layer in bucket；Field planting hole it is evenly distributed with on block of glass wool；Plant in field planting hole and have plant；Liquid-penetrating pipe it is inserted with in Tong；Bottom of the barrel is connected by three-way valve and two collecting pipes；Equipped with resin column on a piece collecting pipe；It is connected with nutrient tank, peristaltic pump bottom two collecting pipes；Nutrient tank is connected with oxygen therapy pump, and peristaltic pump outlet is connected with liquid-penetrating pipe by return duct；Liquid-penetrating pipe end seal tube wall has perforate；Bottom of device is the platform of a provided with wheels, and platform is provided with the support that can fix plant culturing bucket.Method includes the preculture of plant, the collection of root exudates and separation.The present invention has can the features such as categorised collection different component root exudates, external environment isolation are well in situ, integrated degree is high, mobile flexibly, root growth disturbance is little.
Description
Technical field
The invention belongs to plant, soil, agricultural and technical field of environmental science, relate to a kind of plant root exudation
Thing collection device and collection method, particularly to a kind of movable-type secretions from plant roots classified collection device and
Collection method.
Background technology
Secretions from plant roots refer to plant in growth course by root system to surrounding soil discharge a series of
Polarity and nonpolar organic matter matter, including organic acid, aminoacid, saccharide, phenols and fatty acid etc..Root system divides
Secretion can not only reflect the physiological situation of plant self, simultaneously can also activating soil mineral nutrient, produce and plant
Between thing, allelopathy effect, stimulation rhizosphere microorganism activity and removal heavy metal in soil and organic pollution, be dimension
Hold the key factor of rhizosphere microecological system vigor, therefore, in recent years its become plant, soil, agricultural and
The focus of attention of environmental science researcher, and root exudates collection device and the technological development of collection method
It it is research emphasis therein.
According to the difference of culture matrix, conventional root exudates collection method includes that water culture, solid matrix are trained
The method of supporting and earth culture method, said method all has respective pluses and minuses, but involved collection process is basically identical, i.e.
By root system of plant and planted the nutritional solution of plant, solid matrix or soil process solution and extract or drench
Washing, gained extract or leacheate are as root exudates.But, these root exudates collection methods are all deposited
In following problem: the normal growth of interference plant, it is impossible to the secretion situation of root system during reflection plant growth in situ,
And the solution collected comprises the inclusions of root system itself.Nineteen eighty-two, Tang and Young devises root system
Secretions continuously trapping system, constantly circulates the nutritional solution cultivating plant, and utilizes macroporous adsorbing resin for purification
Root exudates therein, thus when realizing plant growth in situ on the basis of not disturbing root growth, root system divides
The collection of secretion.At present, many novel root secretions collection devices are also all based on the design of this device, but
It is that these novel collection devices and corresponding collection method yet suffer from: cannot original position categorised collection opposed polarity root
Being secretions component, the integrated degree of collection system is the highest, mobile dumb, and nutritional solution rate of circulating flow cannot
Strict controlling, system sealing is strong, easily by external environmental interference, and root exudates is collected that the cycle is shorter, received
The problems such as collection amount is little, thus limit the further application of this series of collection device and collection method.
Summary of the invention
It is an object of the invention to solve to design based on above-mentioned root exudates continuously trapping system existing
It it is problem present in secretions collection device and collection method, it is provided that a kind of movable-type secretions from plant roots
Classified collection device and collection method, it has can original position categorised collection different component root exudates, extraneous ring
The features such as border isolation is good, integrated degree is high, move flexibly, root growth disturbance is little.
It is an object of the invention to be achieved through the following technical solutions:
A kind of movable-type secretions from plant roots classified collection device, it includes that plant culturing bucket, root system are secreted
Thing collects part and mobile platform support continuously；
Described plant culturing bucket is made up of opaque material, and top is cylindrical, and bottom is funnel-form, and inwall spreads
There is one layer of coarse filtration net；Fine sand filter course, solid culture substrate it has been sequentially filled from down to up in plant culturing bucket
Layer, block of glass wool and haydite layer；Field planting hole it is evenly distributed with on block of glass wool；Plant in field planting hole and have plant；Plant
Thing cultivation bucket outer wall side is equipped with glass-tube liquid level meter, and opposite side is provided with the liquid-penetrating pipe inserted in plant culturing bucket；
Plant culturing barrel bottom center hole with glass cotton blocking and is wrapped up with finly filtration net；Bottom centre passes through three-way valve in hole
It is connected with two glass collecting pipes；The osculum of a band valve is separately had near bottom centre hole；
Described root exudates collection part continuously include two collecting pipes, resin column, thermal insulation cover, oxygen therapy pump,
Nutrient tank, peristaltic pump, return duct and liquid-penetrating pipe；Two collecting pipes, return ducts are transparent hard tube；Two
Collecting pipe upper end respectively carries a valve, is wherein provided with resin column in the middle of a collecting pipe；Described resin column is one
Root polar resin post, or a non-polar resin post, or polar resin post and another root non-polar resin post
Two resin columns being together in series；The top of described resin column is filled with amphiphilic filter membrane, and the bottom of resin column is used
Finly filtration net is wrapped up, and is provided with thermal insulation cover in resin column periphery；It is connected with three-way valve bottom two collecting pipes,
The three-way valve other end is connected with T-shaped pipe, and T-shaped pipe bottom is the hard tube of a band valve, and hard tube hypomere is through battalion
Nutrient solution tank top rubber stopper inserts in nutrient tank, and the T-shaped pipe other end passes through flexible pipe and peristaltic pump water inlet end phase
Even；Another root hard tube being connected with oxygen therapy pump inserts in nutrient tank also through nutrient tank top rubber plug, defeated
Oxygen pump inlet end is equipped with microbe filter film；Peristaltic pump water side is connected with return duct by flexible pipe；Return duct is another
One end is connected with inserting the liquid-penetrating pipe cultivated in bucket by fluid flowmeter；Liquid-penetrating pipe end seal, length and training
Supporting bucket internal diameter consistent, tube wall has a series of aperture, and pipe shaft is enclosed with gauze；
Described mobile platform frame bottom is a platform, and mesa base is equipped with wheel；Platform is provided with can be fixed
The support of plant culturing bucket, the long spike lower end of support is fixedly mounted on platform；Oxygen therapy pump, nutrient tank and
Peristaltic pump is placed on platform, and fixing plant culturing bucket, collecting pipe, resin column are all located at above nutrient tank.
Described solid culture substrate layer is quartz sand, Vermiculitum or sand.
Described coarse filtration net be grid aperture be the thick nylon filter of 0.1～0.5mm；Described finly filtration net is
Grid aperture is the thin nylon filter of 0.01～0.05mm.
A kind of movable-type secretions from plant roots categorised collection method, comprises the steps:
(1) preculture of plant: be sequentially filled the fine sand cleaned and after sterilizing in plant culturing bucket from down to up
Filter course, solid culture substrate layer (quartz sand, Vermiculitum or sand), rise insulating effect block of glass wool and rise
The haydite layer of fixation, its medium-fine sand filter course thickness is 2～7cm, and fine sand particle diameter is 0.5～1.5mm,
Solid culture substrate layer thickness is 10～50cm, and block of glass wool thickness is 1～5cm, on block of glass wool uniformly
Field planting hole that aperture be 1～3cm is distributed, and haydite layer thickness is 2～5cm；Early stage cultivated is to be measured
Plant seedlings is cleaned, is colonizated in the block of glass wool being evenly distributed with field planting hole after sterilization treatment, closes and cultivates bucket
Bottom centre hole and osculum valve, add nutritional solution until liquidometer liquid level and solid culture substrate layer height one
Cause, maintain this state 5～7 days, make plant Adaptable growth environment；Open the collecting pipe not connecing resin column afterwards
Valve, opens peristaltic pump, enables the nutritional solution in system closely to circulate, with at utmost simulation
The dynamic migration of the soil liquid in actual environment；Can change by observing the reading of fluid flowmeter, regulation is wriggled
Pump discharge, makes the flow velocity of liquid in system remain stable, and peristaltic pump flow adjustment range is 0.05～1500
ml/min；When cultivating bucket Middle nutrition liquid and reducing, available peristaltic pump is drawn nutritional solution from nutrient tank and is supplemented,
And oxygen needed for plant root growth can be supplemented by oxygen therapy pump.
(2) collection of root exudates: according to follow-up study purpose, can collect root at plant different growth phases
It is secretions.When starting to collect root exudates, close the collecting pipe valve not connecing resin column, open and connect resin
The collecting pipe valve of post, makes the nutritional solution containing root exudates carry out closing through the collecting pipe connecing resin column and follows
Ring, the method for operation of closed cycle system is identical with the method for operation during plant preculture；Root system of plant divides
In secretion, polarity and non-polar component pass sequentially through polarity and the absorption of non-polar resin post and are classified collection；Described
Polar resin post can load the macroporous adsorbent resins such as NKA-9, ADS-21 and ADS-F8 type, non-polar resin post
Can load the macroporous adsorbent resins such as Amberlite XAD-4, XAD-16HP and D101 type, the filling of resin column is high
Degree is 10～30cm, resin column a diameter of 2～5cm；Meanwhile, rubber-plastic sponge is installed to protect in resin column periphery
Temperature cover, internal filling ice bag, to ensure the stable in properties of the root exudates being adsorbed on resin during collecting；
The collection cycle of root exudates is generally 3～10 days, and too short acquisition time can cause collecting amount not enough, and
Long acquisition time can cause the degraded of root exudates and the change of composition；
(3) separation of root exudates: take off the resin column being adsorbed with root exudates, by deionized water and first
Alcohol respectively drip washing polarity and non-polar resin, eluent crosses 0.22 μm filter membrane postlyophilization, obtain polarity and
Nonpolar root exudates component；The resin column of drip washing can be reinstalled in collection device and continue on for absorption
Root exudates.
Beneficial effects of the present invention:
The present invention compared with prior art, has the advantage that
(1) resin column of opposed polarity is installed simultaneously, dynamic in-situ categorised collection opposed polarity root system can be realized and divide
Secretion component, it is to avoid subsequent artefacts separates the pollution and loss caused；
(2) it is equipped with mobile platform support, improves plant culturing part and the one of root exudates collection part
Body degree, enhances the operability of equipment, space availability and mobility simultaneously, makes system be placed in
Root exudates is collected under different culture environment；
(3) by build closed nutritional solution circulation line and total system sterilization treatment etc. operate, make system with
External environment isolation is good, and the disturbance to root growth is little, improve collected root exudates verity and
Aseptic；
(4) liquid-penetrating pipe it is installed in blood circulation and is embedded in solid culture substrate, on the one hand can improve cyclic system
The closure of system, on the other hand can make reuse nutritional solution slowly be uniformly distributed in solid culture substrate, improves root
The breathability of system；
(5) the plant preculture stage uses nutritional solution blood circulation, can at utmost soil in simulation actual environment
The dynamic migration of earth solution, creates the root growth environment being close to naturalness；
(6) the rubber-plastic sponge heat-insulating cover of ice bag it is filled with in the installation of resin column periphery, it is ensured that be adsorbed on resin
Root exudates stable in properties under cryogenic, the degraded of root exudates and composition during preventing from collecting
Change, extends the collection cycle of root exudates, adds the collecting amount of root exudates；
(7) effusion meter installed can the changes in flow rate situation of quantitative response circulation line Middle nutrition liquid in time, and lead to
Cross peristaltic pump nutritional solution flow is strictly controlled, maintain the steady of plant growing and root exudates collection system
Fixed, simultaneously can being adjusted the most at any time according to vegetation type and growth characteristics, improve root exudates
Collection efficiency；
(8) plant is after certain time preculture, can be according to follow-up study purpose, when plant difference grows
Collect root exudates in phase or multiple continuous growth stage, improve the controllability that root exudates is collected；
(9) use the thick nylon wire cleaned and fine sand as the defecator in plant culturing bucket, it is to avoid solid
The collection of the loss of culture matrix, beneficially root exudates.
Accompanying drawing explanation
Fig. 1 is the overall structure schematic diagram of apparatus of the present invention；
Fig. 2 is the structural representation of liquid-penetrating pipe.
In figure: 1, treat measuring plants；2, plant culturing bucket；3, haydite layer；4, block of glass wool；5, solid culture
Hypothallus；6, fine sand filter course；7, thick nylon filter；8, glass-tube liquid level meter；9, bottom centre hole；
10, osculum；11, glass cotton；12, thin nylon filter；13, three-way valve；14, plexiglas is not connect
Collecting pipe；15, plexiglas collecting pipe is connect；16, amphiphilic filter membrane；17, polar resin post；18, non-pole
Property resin column；19, rubber-plastic sponge heat-insulating cover；20, thin nylon filter；21, three-way valve；22, T-shaped glass
Glass pipe；23, nutrient tank；24, oxygen therapy pump；25, microbe filter film；26, silica gel hose；27, compacted
Dynamic pump；28, glass return duct；29, fluid flowmeter；30, liquid-penetrating pipe；31, irony platform；32, ten thousand
To wheel；33, iron support
Detailed description of the invention
Below in conjunction with the drawings and specific embodiments, the present invention is further illustrated.
Embodiment 1
As shown in Figure 1 and Figure 2, a kind of movable-type secretions from plant roots classified collection device, it includes planting
Thing is cultivated bucket, root exudates and is collected part and mobile platform support continuously；
Described plant culturing bucket 2 (hereinafter referred to as cultivating bucket) is opaque PVC material, and top is cylindrical；
Bottom is funnel-form, and inwall is covered with the thick nylon filter 7 that one layer of grid aperture is 0.1～0.5mm；Plant
Cultivate to be sequentially filled from down to up in bucket and clean and fine sand filter course 6 after sterilizing, solid culture substrate layer 5
(quartz sand, Vermiculitum or sand), the block of glass wool 4 playing insulating effect and the haydite layer 3 of a fixation；
Its medium-fine sand filter course thickness is 2～7cm, and fine sand particle diameter is 0.5～1.5mm, solid culture substrate thickness
Degree is 10～50cm, and block of glass wool thickness is 1～5cm, and it is 1～3 that block of glass wool is evenly distributed with aperture
The field planting hole of cm, haydite layer thickness is 2～5cm；Cultivation bucket outer wall side is equipped with glass-tube liquid level meter 8, separately
Side is provided with inserts the liquid-penetrating pipe 30 cultivated in bucket；Cultivate barrel bottom center hole 9 block with glass cotton 11 and use
Grid aperture be 0.01～0.05mm thin nylon filter 12 wrap up, bottom centre hole pass through three-way valve 13
It is connected, the end with two glass collecting pipes (do not connect plexiglas collecting pipe 14, connect plexiglas collecting pipe 15)
The osculum 10 of a band valve is separately had near portion's centre bore；
Described root exudates collection part continuously includes that two glass collecting pipes (do not connect plexiglas collecting pipe
14, connect plexiglas collecting pipe 15), resin column (polar resin post 17, the nonpolar tree of two opposed polarities
Fat post 18), rubber-plastic sponge heat-insulating cover 19, oxygen therapy pump 24, nutrient tank 23, peristaltic pump 27, glass backflow
Pipe 28 and liquid-penetrating pipe 30；Two glass collecting pipe upper ends respectively carry a valve, wherein in a glass collecting pipe
Between be sequentially installed with the resin column of two opposed polarities, above the top of a resin column fill 0.45 μm parents
Property filter membrane 16, below the thin nylon filter that grid aperture, bottom is 0.01～0.05mm of a resin column
Net 20 is wrapped up, and is provided with rubber-plastic sponge heat-insulating cover 19 two resin column peripheries, at the bottom of two glass collecting pipes
Portion is connected with three-way valve 21, and three-way valve 21 other end is connected with T-shaped glass tubing 22；T-shaped glass tubing 22 times
Portion is the glass tubing of a band valve, and glass tubing hypomere inserts nutrient tank through nutrient tank top rubber plug
In 23, the T-shaped glass tubing other end is connected with peristaltic pump 27 water inlet end by silica gel hose；With oxygen therapy pump 24
Another root glass tubing being connected inserts in nutrient tank 23 also through nutrient tank top rubber plug, cures by oxygen therapy pump 24
Inlet end fills 0.22 μm microbe filter film 25；Silica gel hose 26 and glass are passed through in peristaltic pump 27 water side
Return duct 28 is connected；The flow of peristaltic pump is adjustable, and range of accommodation is 0.05～1500ml/min；Glass refluxes
Pipe 28 other end is connected with inserting the liquid-penetrating pipe 30 cultivated in bucket by fluid flowmeter 29；Liquid-penetrating pipe is PVC
Material, end seal, length is consistent with cultivating bucket internal diameter, and caliber is 2.5cm, and tube wall longitudinally equidistantly opens 6
Row's aperture, aperture is 0.5cm, and pipe shaft is enclosed with 2 layers of gauze；
Described mobile platform frame bottom is irony platform 31, and irony mesa base is provided with four strap brakes
The universal wheel 32 of sheet；Irony platform is provided with the iron support 33 that can fix plant culturing bucket, iron support
Four long spike lower ends are fixedly mounted on four limit central points of irony platform；Oxygen therapy pump, nutrient tank and wriggling
Pump is placed on irony platform, and fixing plant culturing bucket, collecting pipe, resin column are all located at above nutrient tank.
Embodiment 2: the collection of Herba Apii graveolentis root exudates
In plant culturing bucket 2, one layer of grid aperture of paving is the thick nylon filter 7 of 0.5mm, at drainage screen
Upper berth a layer thickness is the fine sand filter course 6 of 5cm, and fine sand particle diameter is 1mm, loads on fine sand filter course
The solid culture substrate layer 5 (quartz sand) that 20cm is thick, and liquid-penetrating pipe is embedded in wherein, on hypothallus upper berth one
Block is evenly distributed with the block of glass wool 4 that aperture is 1.5cm field planting hole, and paving a layer thickness is 2cm's thereon
Haydite layer 3.To treat that measuring plants 1 (Herba Apii graveolentis seedling) is colonizated in above-mentioned cultivation bucket, close plant culturing drum head
Portion's centre bore and osculum valve, addition nutritional solution is saturated to quartz sand culture matrix layer, and maintains this state 7
My god；Connecting the collecting pipe not connecing resin column afterwards, open peristaltic pump, the circular flow of regulation nutritional solution is 1
Ml/min, continues preculture Herba Apii graveolentis 3 weeks under this operational factor.When Herba Apii graveolentis enters vigorous period, start
Collect its root exudates, now, close the collecting pipe not connecing resin column, open the collecting pipe installing resin column,
Filled resin column is followed successively by the polar macroporous resin column of NKA-9 of height 20cm from top to bottom with height 18cm's
Amberlite XAD-4 non-polar macroporous resin post, resin column diameter is 2.5cm, meanwhile, outside resin column
Enclose and the rubber-plastic sponge heat-insulating cover filling ice bag is installed.Under conditions of the circular flow of nutritional solution is 1ml/min,
Collect Herba Apii graveolentis root exudates 5 days, close peristaltic pump afterwards, take off polarity and non-polar resin post, use respectively
Deionized water and methanol drip washing polarity and non-polar resin, eluent crosses 0.22 μm filter membrane postlyophilization,
To polarity and nonpolar Herba Apii graveolentis root exudates component.The each parts of collection device, planter box filled media, suction
Attached resin, nutritional solution and plant seedlings the most all carry out cleaning, sterilization treatment.
Embodiment 3: the collection of soybean root system secretions
In plant culturing bucket, one layer of grid aperture of paving is the thick nylon filter of 0.3mm, on drainage screen upper berth
A layer thickness is the fine sand filter course of 3cm, and fine sand particle diameter is 0.5mm, loads 30cm on fine sand filter course
Thick Vermiculitum culture matrix layer, and liquid-penetrating pipe is embedded in wherein, being evenly distributed with aperture on one piece of hypothallus upper berth is
The block of glass wool of 2cm field planting hole, and paving a layer thickness is the haydite layer of 5cm thereon.Soybean seedling is fixed
Plant in above-mentioned cultivation bucket, close barrel bottom center hole and osculum valve, add nutritional solution to Vermiculitum culture medium
Matter layer is saturated, and maintains this state 5 days, connects the collecting pipe not connecing resin column afterwards, opens peristaltic pump, adjusts
The circular flow of joint nutritional solution is 0.5ml/min, continues preculture Semen sojae atricolor 4 weeks under this operational factor.Treat big
When bean enters out phase, start to collect its root exudates, now, close the collecting pipe not connecing resin column, beat
Opening the collecting pipe installing resin column, filled resin column is followed successively by the ADS-21 polarity of height 15cm from top to bottom
Macroporous resin column and the Amberlite XAD-16HP non-polar macroporous resin post of height 10cm, resin column diameter
It is 4cm, meanwhile, the rubber-plastic sponge heat-insulating cover filling ice bag is installed in resin column periphery.At nutritional solution
Under conditions of circular flow is 0.5ml/min, collects soybean root system secretions 7 days, close peristaltic pump afterwards,
Take off polarity and non-polar resin post, respectively with deionized water and methanol drip washing polarity and non-polar resin, eluting
Liquid crosses 0.22 μm filter membrane postlyophilization, obtains polarity and nonpolar soybean root system secretions component.Collect
The each parts of device, planter box filled media, adsorbent resin, nutritional solution and plant seedlings are the most all washed
Only, sterilization treatment.
Embodiment 4: the collection of Alfalfa Root System secretions
In plant culturing bucket, one layer of grid aperture of paving is the thick nylon filter of 0.2mm, on drainage screen upper berth
A layer thickness is the fine sand filter course of 7cm, and fine sand particle diameter is 1.5mm, loads 40cm on fine sand filter course
Thick sand culture matrix layer, and liquid-penetrating pipe is embedded in wherein, being evenly distributed with aperture on one piece of hypothallus upper berth is
The block of glass wool of 3cm field planting hole, and paving a layer thickness is the haydite layer of 3cm thereon.By alfalfa children
Seedling is colonizated in above-mentioned cultivation bucket, closes barrel bottom center hole and osculum valve, adds nutritional solution and trains to Vermiculitum
Support hypothallus saturated, and maintain this state 7 days, connect the collecting pipe not connecing resin column afterwards, open peristaltic pump,
The circular flow of regulation nutritional solution is 1.5ml/min, continues preculture Semen sojae atricolor 6 weeks under this operational factor.Treat
When alfalfa enters initial bloom stage, start to collect its root exudates, now, close the collection not connecing resin column
Pipe, opens the collecting pipe installing resin column, and filled resin column is followed successively by the ADS-F8 of height 10cm from top to bottom
Polar macroporous resin column and the D101 non-polar macroporous resin post of height 20cm, resin column diameter is 2cm,
Meanwhile, the rubber-plastic sponge heat-insulating cover of filling ice bag is installed in resin column periphery.Circular flow at nutritional solution is
Under conditions of 1.5ml/min, collect soybean root system secretions 9 days, close peristaltic pump afterwards, take off polarity and
Non-polar resin post, respectively with deionized water and methanol drip washing polarity and non-polar resin, eluent crosses 0.22
μm filter membrane postlyophilization, obtains polarity and nonpolar Alfalfa Root System secretions component.Collection device
Each parts, planter box filled media, adsorbent resin, nutritional solution and plant seedlings the most all carry out cleaning,
Sterilization treatment.
Claims (14)
1. a movable-type secretions from plant roots classified collection device, it is characterised in that it includes plant
Cultivate bucket, root exudates and collect part and mobile platform support continuously；
Described plant culturing bucket is made up of opaque material, and top is cylindrical, and bottom is funnel-form, and inwall spreads
There is one layer of coarse filtration net；Fine sand filter course, solid culture substrate it has been sequentially filled from down to up in plant culturing bucket
Layer, block of glass wool and haydite layer；Field planting hole it is evenly distributed with on block of glass wool；Plant culturing bucket outer wall side fills
Glass-tube liquid level meter, opposite side is had to be provided with the liquid-penetrating pipe inserted in plant culturing bucket；Plant culturing barrel bottom center
Hole with glass cotton blocking and is wrapped up with finly filtration net；Bottom centre hole is connected by three-way valve and two glass collecting pipes
Connect；The osculum of a band valve is separately had near bottom centre hole；
Described root exudates collection part continuously include two collecting pipes, resin column, thermal insulation cover, oxygen therapy pump,
Nutrient tank, peristaltic pump, return duct and liquid-penetrating pipe；Two collecting pipes, return ducts are transparent hard tube；Two
Collecting pipe upper end respectively carries a valve, is wherein provided with resin column in the middle of a collecting pipe；Described resin column is one
Root polar resin post, or a non-polar resin post, or polar resin post and another root non-polar resin post
Two resin columns being together in series；The top of described resin column is filled with amphiphilic filter membrane, and the bottom of resin column is used
Finly filtration net is wrapped up, and is provided with thermal insulation cover in resin column periphery；It is connected with three-way valve bottom two collecting pipes,
The three-way valve other end is connected with T-shaped pipe, and T-shaped pipe bottom is the hard tube of a band valve, and hard tube hypomere is through battalion
Nutrient solution tank top rubber stopper inserts in nutrient tank, and the T-shaped pipe other end passes through flexible pipe and peristaltic pump water inlet end phase
Even；Another root hard tube being connected with oxygen therapy pump inserts in nutrient tank also through nutrient tank top rubber plug, defeated
Oxygen pump inlet end is equipped with microbe filter film；Peristaltic pump water side is connected with return duct by flexible pipe；Return duct is another
One end is connected with inserting the liquid-penetrating pipe cultivated in bucket by fluid flowmeter；Liquid-penetrating pipe end seal, length and training
Supporting bucket internal diameter consistent, tube wall has a series of aperture, and pipe shaft is enclosed with gauze；
Described mobile platform frame bottom is a platform, and mesa base is equipped with wheel；Platform is provided with can be fixed
The support of plant culturing bucket, the long spike lower end of support is fixedly mounted on platform；Oxygen therapy pump, nutrient tank and
Peristaltic pump is placed on platform, and fixing plant culturing bucket, collecting pipe, resin column are all located at above nutrient tank.
2. movable-type secretions from plant roots classified collection device as claimed in claim 1, its feature exists
In, described solid culture substrate layer is quartz sand, Vermiculitum or sand.
3. movable-type secretions from plant roots classified collection device as claimed in claim 2, its feature exists
In, described plant culturing bucket is opaque PVC material；Described thermal insulation cover is rubber-plastic sponge heat-insulating cover.
4. movable-type secretions from plant roots classified collection device as claimed in claim 3, its feature exists
In, described coarse filtration net be grid aperture be the thick nylon filter of 0.1～0.5mm；Described finly filtration net is
Grid aperture is the thin nylon filter of 0.01～0.05mm.
5. movable-type secretions from plant roots classified collection device as claimed in claim 1, its feature exists
In, the film hole diameter of described amphiphilic filter membrane is 0.45 μm；The film hole diameter of described microbe filter film is 0.22
μm。
6. movable-type secretions from plant roots classified collection device as claimed in claim 1, its feature exists
In, fine sand filter course thickness is 2～7cm, and fine sand particle diameter is 0.5～1.5mm, solid culture substrate thickness
Degree is 10～50cm, and block of glass wool thickness is 1～5cm, and it is 1～3 that block of glass wool is evenly distributed with aperture
The field planting hole of cm, haydite layer thickness is 2～5cm.
7. movable-type secretions from plant roots classified collection device as claimed in claim 1, its feature exists
In, liquid-penetrating pipe is PVC material, and caliber is 2.5cm；Liquid-penetrating pipe tube wall longitudinally equidistantly opens six row's apertures, hole
Footpath is 0.5cm, and pipe shaft is enclosed with 2 layers of gauze.
8. movable-type secretions from plant roots classified collection device as claimed in claim 1, its feature exists
In, described polar resin post filling NKA-9, ADS-21 or ADS-F8 type macroporous adsorbent resin, nonpolar tree
Fat post filling Amberlite XAD-4, XAD-16HP or D101 type macroporous adsorbent resin, the filling of resin column is high
Degree is 10～30cm, resin column a diameter of 2～5cm.
9. movable-type secretions from plant roots classified collection device as claimed in claim 1, its feature exists
It is glass in, two collecting pipes, T-shaped pipe, two hard tubes, return ducts through nutrient tank top rubber plug
Glass material；Described flexible pipe is silica gel hose；The flow of peristaltic pump is adjustable, and range of accommodation is 0.05～1500
ml/min。
10. movable-type secretions from plant roots classified collection device as claimed in claim 1, its feature exists
In, described platform is irony platform；Described support is iron support；Mesa base is provided with four strap brake sheets
Universal wheel.
The movable-type secretions from plant roots classification that 11. 1 kinds utilize as described in claim 1-10 is arbitrary is received
The secretions from plant roots categorised collection method that acquisition means is carried out, it is characterised in that comprise the steps:
(1) preculture of plant: be sequentially filled the fine sand cleaned and after sterilizing in plant culturing bucket from down to up
Filter course, solid culture substrate layer, block of glass wool and haydite layer, the plant seedlings to be measured early stage cultivated is washed
Only, it is colonizated in the block of glass wool being evenly distributed with field planting hole after sterilization treatment, closes and cultivate barrel bottom center hole
With osculum valve, addition nutritional solution is until liquidometer liquid level is consistent with solid culture substrate layer height, and maintaining should
State 5～7 days, make plant Adaptable growth environment；Open the collecting pipe valve not connecing resin column afterwards, open
Peristaltic pump, enables the nutritional solution in system closely to circulate, with at utmost simulation actual environment
The dynamic migration of the soil liquid；Can change by observing the reading of fluid flowmeter, regulate peristaltic pump flow, make
In system, the flow velocity of liquid remains stable；When cultivating bucket Middle nutrition liquid and reducing, available peristaltic pump is from nutrient tank
Middle absorption nutritional solution supplements, and can supplement oxygen needed for plant root growth by oxygen therapy pump；
(2) collection of root exudates: according to follow-up study purpose, can collect root at plant different growth phases
It is secretions；When starting to collect root exudates, close the collecting pipe valve not connecing resin column, open and connect resin
The collecting pipe valve of post, makes the nutritional solution containing root exudates carry out closing through the collecting pipe connecing resin column and follows
Ring, the method for operation of closed cycle system is identical with the method for operation during plant preculture；Root system of plant divides
In secretion, polarity and non-polar component pass sequentially through polarity and the absorption of non-polar resin post and are classified collection；With
Time, rubber-plastic sponge heat-insulating cover is installed in resin column periphery, internal filling ice bag, to ensure to adsorb during collecting
The stable in properties of the root exudates on resin；The collection cycle of root exudates is generally 3～10 days, mistake
Short acquisition time can cause collecting amount not enough, and long acquisition time can cause root exudates degraded and
The change of composition；
(3) separation of root exudates: take off the resin column being adsorbed with root exudates, by deionized water and first
Alcohol respectively drip washing polarity and non-polar resin, eluent crosses 0.22 μm filter membrane postlyophilization, obtain polarity and
Nonpolar root exudates component；The resin column of drip washing can be reinstalled in collection device and continue on for absorption
Root exudates.
12. secretions from plant roots categorised collection methods as claimed in claim 11, it is characterised in that
Fine sand filter course thickness is 2～7cm, and fine sand particle diameter is 0.5～1.5mm, solid culture substrate thickness
Degree is 10～50cm, and block of glass wool thickness is 1～5cm, and it is 1～3 that block of glass wool is evenly distributed with aperture
The field planting hole of cm, haydite layer thickness is 2～5cm.
13. secretions from plant roots categorised collection methods as claimed in claim 11, it is characterised in that
Described polar resin post filling NKA-9, ADS-21 or ADS-F8 type macroporous adsorbent resin, nonpolar tree
Fat post filling Amberlite XAD-4, XAD-16HP or D101 type macroporous adsorbent resin, the filling of resin column is high
Degree is 10～30cm, resin column a diameter of 2～5cm.
14. secretions from plant roots categorised collection methods as claimed in claim 11, it is characterised in that compacted
The flow of dynamic pump is adjustable, and range of accommodation is 0.05～1500ml/min.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108519250A (en) * | 2018-04-18 | 2018-09-11 | 中国科学院水生生物研究所 | It is a kind of to collect submerged plant root exudates device and application method in situ |
| CN109406244A (en) * | 2018-12-29 | 2019-03-01 | 中国科学院南京地理与湖泊研究所 | A kind of water plant root exudates original position collection device |
| CN112539969A (en) * | 2020-11-30 | 2021-03-23 | 赣州久创科技有限公司 | Continuous collection device of plant root system secretion |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108519250A (en) * | 2018-04-18 | 2018-09-11 | 中国科学院水生生物研究所 | It is a kind of to collect submerged plant root exudates device and application method in situ |
| CN108519250B (en) * | 2018-04-18 | 2024-02-27 | 中国科学院水生生物研究所 | Device for collecting submerged plant root secretions in situ and use method |
| CN109406244B (en) * | 2018-12-29 | 2023-10-10 | 中国科学院南京地理与湖泊研究所 | Aquatic plant root system secretion normal position collection device |
| CN109406244A (en) * | 2018-12-29 | 2019-03-01 | 中国科学院南京地理与湖泊研究所 | A kind of water plant root exudates original position collection device |
| CN112539969A (en) * | 2020-11-30 | 2021-03-23 | 赣州久创科技有限公司 | Continuous collection device of plant root system secretion |
| CN112539969B (en) * | 2020-11-30 | 2023-11-14 | 赣州久创科技有限公司 | Continuous collection device of plant root secretion |
| CN112557113A (en) * | 2020-12-14 | 2021-03-26 | 东北林业大学 | Combined in-situ root exudate collector and collecting method thereof |
| CN112557113B (en) * | 2020-12-14 | 2024-03-22 | 东北林业大学 | Combined in-situ root system secretion collector and collecting method thereof |
| CN113218700A (en) * | 2021-04-29 | 2021-08-06 | 河海大学 | Membrane collection device and method for plant root exudates in soil culture mode |
| CN114112480A (en) * | 2021-12-17 | 2022-03-01 | 贵州省生物研究所 | Device and method for collecting pepper rhizosphere secretion |
| CN114323788B (en) * | 2021-12-23 | 2023-05-16 | 华南师范大学 | Plant root system secretion in-situ collection device and method for collecting plant root system secretion by adopting same |
| CN114323788A (en) * | 2021-12-23 | 2022-04-12 | 华南师范大学 | Plant root exudate in-situ collection device and method for collecting plant root exudate by using same |
| CN117760796A (en) * | 2024-02-22 | 2024-03-26 | 吉林大观生态环境科技有限公司 | Plant root secretion collection device |
| CN117760796B (en) * | 2024-02-22 | 2024-05-17 | 吉林大观生态环境科技有限公司 | Plant root secretion collection device |
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