CN110672351A

CN110672351A - Device and method for collecting root exudates of gramineous plants

Info

Publication number: CN110672351A
Application number: CN201911085659.0A
Authority: CN
Inventors: 连腾祥; 年海; 刘俊杰; 马启彬; 程艳波; 蔡占东; 曹亚琴
Original assignee: South China Agricultural University
Current assignee: South China Agricultural University
Priority date: 2019-11-08
Filing date: 2019-11-08
Publication date: 2020-01-10
Anticipated expiration: 2039-11-08
Also published as: CN110672351B

Abstract

The invention discloses a device and a method for collecting root exudates of gramineous plants. The plant root exudate collecting device comprises a root box; 2 root secretion collecting grooves which are arranged oppositely are arranged in the root box; the top of the root secretion collecting tank is provided with an opening; all be equipped with the through-hole on 2 root system secretion collecting vat's 4 vertical side walls of parallel arrangement, the through-hole is sealed by the net thing. The invention provides a method for collecting plant root exudates, which comprises the following steps: filling soil in a root box and a root exudate collecting tank in the plant root exudate collecting device; plant the plant between 2 root exudates collecting grooves, plant roots grows, and partial root system gets into the root exudates collecting groove through the open-top of grid thing and root exudates collecting groove in, can collect plant roots exudates from the soil in the root exudates collecting groove. The method does not need to damage the roots of the plants when collecting the root exudates, thereby increasing the reliability of the experiment.

Description

Device and method for collecting root exudates of gramineous plants

Technical Field

The invention relates to a device and a method for collecting root exudates of gramineous plants, belonging to the technical field of soil and plant science.

Background

The root secretion is a general name of all substances secreted by a root system to a culture medium in an active or passive mode in the growth process of the plant, and comprises organic/inorganic ions, plant hormones, root cell desquamation products, decomposition products of the root cell desquamation products and the like, the synthesis and secretion process of the root secretion is an important way for realizing the interaction between the plant and the rhizosphere environment, the properties of rhizosphere soil can be improved, the community composition of rhizosphere microorganisms can be regulated, and the plant can feedback regulate the growth of the plant by sensing the change of the root secretion and the rhizosphere microorganisms.

There is no uniform standard for the root exudate collection method, and most studies are to collect the root exudate by using a solution culture collection method or a matrix culture collection method. However, both methods change the normal growth environment of the plant and cannot reduce the actual secretion of the plant in the soil. Research reports that the method for collecting root exudates by soil culture is more troublesome than solution culture and collection, plants are planted in soil, the root soil is directly obtained after the plants grow for a period of time, the root soil and sterile water are mixed according to a certain proportion, and the obtained filtrate is the root exudates. However, the obtained substances obtained by the method contain original complex compounds in the soil, and in addition, the root system washing method is characterized in that the root system is damaged in the washing process after the root system is dug out, so that the collected root system secretion, which contains substances and bleeding sap in the root system body, can greatly influence the experimental result.

Disclosure of Invention

The invention aims to provide a device and a method for collecting root exudates of gramineous plants, and provides an effective method for effectively collecting the root exudates and reflecting the real situation of the root exudates in soil.

The invention provides a plant root secretion collecting device, which comprises a root box; 2 root secretion collecting grooves which are oppositely arranged are arranged in the root box;

the top of the root exudate collecting tank is provided with an opening;

2 all be equipped with the through-hole on 4 perpendicular lateral walls of the parallel arrangement of root system secretion collecting vat, the through-hole is sealed by the net thing.

During the use, be used for filling soil in the root exudates collecting vat, utilize the net thing blocks outside soil and gets into in the root exudates collecting vat, and plant roots can grow wherein and release the root exudates.

In the plant root exudate collection device, the root exudate collection tank is hexagonal, so that the plant root can grow in the root exudate collection tank better, and the root exudate can be collected in a large amount.

In the device for collecting the plant root secretion, the root secretion collecting tank is arranged, the width of the vertical side wall of the through hole is larger than the width of the rest 2 vertical side walls, namely, the grid object is arranged on the vertical side wall of the wide part, so that the root system enters the root secretion collecting tank as much as possible.

In the device for collecting the plant root secretion, the through hole is formed in the middle of the vertical side wall.

In the device for collecting the plant root exudates, the root exudates collecting tank is surrounded by the PVC plate, so that the device is waterproof, not easy to rot and low in cost;

the net thing can be the nylon wire, can make water and root secretion and microorganism pass, and can effectively keep apart soil and get into in the root secretion collecting vat, guaranteed the purity of root secretion.

The average pore size of the mesh was 48 μm to allow root access but not soil access.

Based on the plant root secretion collecting device, the invention further provides a plant root secretion collecting method, which comprises the following steps:

filling soil in the root box and the root exudate collecting tank in the plant root exudate collecting device;

plant the plant between the root exudates collecting vat, plant roots grows, and partial root system passes through the grid with the open-top of root exudates collecting vat gets into in the root exudates collecting vat, can follow collect plant roots exudates in the soil in the root exudates collecting vat.

Plant root exudate can be collected according to the following steps: and adding water or PBS buffer solution into the root secretion collecting tank, and collecting liquid after the plant root secretion is released into the root secretion collecting tank (the secretion can be released (migrated) into the water or PB buffer solution), namely collecting the plant root secretion.

The liquid may be collected in particular by means of a syringe.

The method of the invention can collect the root secretion of gramineous plants.

And planting the plants in the middle of the 2 root exudate collecting grooves.

The invention utilizes the root secretion collecting tank to collect the root secretion, in particular to a device which is used for plant root to grow and release the root secretion, during the collection, water or PBS buffer solution is added into the root secretion collecting tank to dissolve the root secretion, and then liquid is collected by adopting an injector and the like. According to the method, the roots of the plants are not required to be damaged when the root exudates are collected, more root exudates can be collected in the micro-domain of the collecting device, and the reliability of the experiment is improved; in addition, the collecting device is convenient for sampling after the experiment is finished, for example, the mixed liquid of the root exudates and the PBS buffer solution at the bottom of the tank can be directly pumped out by an injector and then refrigerated for testing.

Drawings

FIG. 1 is a schematic view showing the structure of a secretion collecting apparatus for a plant root system according to the present invention (in a use state).

FIG. 2 is a result view of the collecting tank for root exudates in the apparatus for collecting root exudates of plants according to the present invention.

FIG. 3 shows the metabolite metabolism components of the detected sample obtained by the device for collecting the plant root secretion of the present invention.

The respective symbols in the figure are as follows:

1 root box, 2 root secretion collecting tanks, 3 nylon wire netting.

Detailed Description

The present invention will be further described with reference to the accompanying drawings, but the present invention is not limited to the following embodiments.

As shown in fig. 1, the structure of the device for collecting plant root exudates of the present invention is schematically illustrated, and the device includes a

root box

1, and 2 collecting tanks 2 for collecting plant root exudates are disposed in the root box 1. As shown in fig. 2, the root exudate collecting tank 2 is hexagonal, the top of the root exudate collecting tank is provided with an opening, and the root exudate collecting tank 2 is enclosed by a PVC plate). The middle parts of the wider vertical side walls of the root exudate collecting tank 2 are provided with through holes, the through holes are sealed by nylon nets 3, and the average pore diameter of the nylon nets 3 is 48 mu m.

When the device is used for collecting root exudates, the method can be carried out according to the following steps:

the root box 1 and the root exudate collecting tank 2 in the plant root exudate collecting device are filled with soil. Then plant the plant between 2 root exudates collecting vat 2, plant roots grows, and partial root system can get into root exudates collecting vat 2 through nylon wire 3 and root exudates collecting vat 2's open-top, then releases the root exudates in to root exudates collecting vat 2. And (3) when the plant is in a full-bloom stage (the most vigorous root secretion stage), collecting the root secretion in the root secretion collecting tank 2, treating the root secretion with liquid nitrogen, storing the root secretion in an environment at the temperature of minus 80 ℃, and detecting the metabolomics.

The collecting effect of the device according to the invention is illustrated below by a specific example:

control 2 root secretion collecting vat 2's interval is 2 ~ 20cm, plants the soybean among them, plant root system growth, and partial root system then can get into root secretion collecting vat 2 through the open-top of nylon net 3 and root secretion collecting vat 2 to in releasing root secretion collecting vat 2, the root secretion who grows outside root secretion collecting vat 2 also can release root secretion collecting vat 2 through nylon net 3. In the period of most vigorous root secretions, collection is carried out: add water in to root exudates collecting vat 2, after plant root exudates released root exudates collecting vat 2, utilize the syringe to collect liquid and collect promptly and obtain the root exudates, detect secretion metabolic component, the result is shown as figure 3, can see out that this method can obtain comparatively complete and enough kind quantity's metabolite, and follow-up analysis can normally be carried out to its result.

When the plant root exudate collecting device is used for collecting the plant root exudate, the plant grows in the soil, and the environment of the plant growing in the soil is restored to a certain degree, so that the plant root exudate can be collected under the condition of not collecting the soil, and the influence of the soil on the plant root exudate is avoided.

Claims

1. A plant root secretion collecting device comprises a root box; the method is characterized in that: 2 root secretion collecting grooves which are oppositely arranged are arranged in the root box;

the top of the root exudate collecting tank is provided with an opening;

2. The plant root exudate collection device of claim 1, wherein: the root exudate collecting tank is in a hexagonal shape.

3. The plant root exudate collection device of claim 2, wherein: the root exudate collecting groove is provided with the width of the vertical side wall of the through hole is larger than the width of the rest 2 vertical side walls.

4. A plant root exudate collection apparatus as claimed in any one of claims 1 to 3 wherein: the through hole is formed in the middle of the vertical side wall.

5. The plant root exudate collection device of any one of claims 1-4, wherein: the root secretion collecting tank is enclosed by a PVC plate;

the mesh is a nylon mesh;

the average pore size of the mesh was 48 μm.

6. A method for collecting plant root exudates comprises the following steps:

filling the root box and the root exudate collection tank with soil in the plant root exudate collection device of any one of claims 1-5;

plant the plant between the root exudates collecting vat, plant roots grows, and partial plant roots passes through the grid with the open-top of root exudates collecting vat gets into in the root exudates collecting vat, can follow collect plant roots exudates in the soil in the root exudates collecting vat.

7. The method for collecting plant root exudates according to claim 6, wherein: the plant is a gramineous plant.

8. The method for collecting plant root exudates according to claim 6 or 7, wherein: and planting the plants in the middle of the 2 root exudate collecting grooves.

9. The method for collecting plant root exudate as claimed in any one of claims 6 to 8, wherein: and adding water or PBS buffer solution into the root secretion collecting tank, and collecting liquid to collect the plant root secretion after the plant root secretion is released into the root secretion collecting tank.

10. The method for collecting plant root exudates according to claim 9, wherein: the liquid was collected using a syringe.