CN104006988A - Conical surface percolation type device and method for collecting root exudates of soil cultivation plants - Google Patents
Conical surface percolation type device and method for collecting root exudates of soil cultivation plants Download PDFInfo
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- CN104006988A CN104006988A CN201410223952.XA CN201410223952A CN104006988A CN 104006988 A CN104006988 A CN 104006988A CN 201410223952 A CN201410223952 A CN 201410223952A CN 104006988 A CN104006988 A CN 104006988A
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Abstract
The invention discloses conical surface percolation type device and method for collecting root exudates of soil cultivation plants. The collection device comprises a growing box and a collection box, wherein holes are formed in the outer wall of the growing box and are used for filling agar. A collection hole is formed in a connection part of the cylindrical growing box and the lower end of the collection box; the lower end of the whole box body is provided with a rubber plug with a hole and is connected with a resin column by a guide pipe. The novel collection method for the root exudates of the soil cultivation plants comprises the following steps: firstly, enabling the plants to grow in soil of the growing box; enabling plant roots to penetrate through the agar to enter the collection box filled with quartz sand; treating and collecting the root exudates of the plants in the collection box. The conical surface percolation type device and method are suitable for researching the root exudates of the plants under soil cultivation conditions; the growth of the roots is not disturbed and a root environment is not damaged; a collection environment is close to a natural growing state of the plants; the collection method has a small occupied area and the available growing space of the plants is large. The method is low in cost and strong in practicability and has a very good application value.
Description
Technical field
The present invention relates to soil pollution environment phytoremediation technology field, particularly a kind of circular conical surface percolation type is collected the apparatus and method of secretion from root system of plant in pot.
Background technology
At present, secretions from plant roots generally adopts solution collecting method, and concrete operation method is: root system of plant is first grown in nutrient solution, then proceeds in Treatment Solution, processes after a period of time, and collection and treatment solution is root exudates.Solution collecting method is simple to operate, easy analysis.But the root exudates that solution collecting method obtains is difficult to secretions from plant roots under reflect soil condition.Because soil constituent is complicated, nutrient existence form diversified, skewness, and, most of available nutrient is adsorbed by soil colloid, can not arbitrarily spread, this shows under earth culture condition, root system of plant suffers the actual conditions of nutrient dificiency or element toxicity to be different from hydroponics, thereby soil cultivation may be different from the root exudation under hydroponics condition.In excretion collecting device for root system of plant and collection method and gathering-device under the soil cultivation condition that at present existing patent adopts, some uses are loaded down with trivial details, and some floor areas are large and usage space is little, and the number of plant of plantation is very limited.
Summary of the invention
The object of the invention is to overcome the shortcoming existing in existing root exudates gathering-device, gathering-device and the collection method of the root exudates that the apparatus and method that provide a kind of circular conical surface percolation type to collect secretion from root system of plant in pot are a kind of complete, efficient not disturbance root systems, do not destroy rhizospheric environment, reproducible, the recovery is high.
Object of the present invention is implemented by following technical proposal:
A kind of circular conical surface percolation type is collected the device of secretion from root system of plant in pot, comprise growth case and collecting box, in growth case, place for examination soil, on the outer wall of described growth case, have hole, and fill with agar, described growth case and junction, collecting box lower end are provided with collection hole, described collection hole lower end connects nylon wire, described nylon wire lower end connecting duct, conduit connects respectively rubber stopper with holes, switch and resin column from top to bottom, and the conduit of resin column lower end takes back collecting box top and places silica sand place.
Described growth case and collecting box are cylindrical, and described growth box outer wall is from apart from 100mm place, top to having hole, aperture 6mm, and pitch of holes is 4mm.Described silica sand diameter is 2 ~ 3mm.
A kind of circular conical surface percolation type is collected the method for secretion from root system of plant in pot, comprise the steps: to treat measuring plants growing in soil for trying in growth case, due to growing space restriction and Action of Gravity Field, root system of plant to be measured is through the agar in growth box outer wall hole, enter in the collecting box that silica sand is housed, until collecting box accumulation, have after certain root system of plant, carry out the collection of root exudates; Then with eluent, the root system being grown in silica sand is carried out to wash-out, flow out under solution seepage flow in resin column, and by resin adsorption; Last wash-out resin, obtains root exudates solution.Before carrying out the collection of root exudates, need first with the calcium liquid of 0.5mM, clean collecting box.
Compared with prior art, tool has the following advantages and effect in the present invention:
Apparatus of the present invention and method are applicable to the research of secretions from plant roots under soil cultivation condition, compare the actual conditions that more can reflect secretions from plant roots under natural conditions with water planting.Not disturbance of the present invention root growth, do not destroy rhizospheric environment, collect environment and more press close to plant self-sow state, avoided soil liquid sampler because of indefinite root system position, sampling blindly, the shortcoming of easy damaged root system.Grow case and collecting box of the present invention is cylindrical, and the relatively good utilization in space, can plant the plant of a greater number, and collected like this root exudates composition and quantity are also more complete.This collection method floor area is little, and the available growing space of plant is large, and cost is low, practical, has good using value.
Accompanying drawing explanation
Fig. 1 is the one-piece construction schematic diagram of gathering-device of the present invention.
Embodiment
Below in conjunction with embodiment, the present invention is done to further detailed description, but embodiments of the present invention are not limited to this.
As shown in Figure 1, a kind of circular conical surface percolation type is collected the device of secretion from root system of plant in pot, comprise growth case 1 and collecting box 2, in growth case 1, place for examination soil 3, on the outer wall of described growth case 1, have hole 4, and fill with agar, described growth case 1 and collecting box 2 junctions, lower end are provided with collection hole 11, described collection hole 11 lower ends connect nylon wire 7, described nylon wire 7 lower end connecting ducts 9, conduit 9 connects respectively rubber stopper 5 with holes, switch 6 and resin column 8 from top to bottom, and the conduit 9 of resin column 8 lower ends takes back collecting box 2 tops and places silica sand 10 places.
Described growth case 1 is right cylinder, external diameter 160mm, wall thickness 3mm.Described collecting box 2 is right cylinder, external diameter 240mm, wall thickness 3mm.The aperture of collecting lower end collection hole 11 is 6mm.Described growth case 1 outer wall is from apart from 100mm place, top to having hole 4, aperture 6mm, and pitch of holes is 4mm.Described silica sand 10 diameters are 2 ~ 3mm.
Nylon wire 7 is used for preventing that silica sand 10 from leaking.Rubber stopper 5 with holes, for effective connection of different parts, seals.The horminess glass tube that resin column 8 is chemical inertness.Resin is XAD-4 polymeric adsorbent.
A kind of circular conical surface percolation type is collected the method for secretion from root system of plant in pot, comprise the steps: first will be placed in growth case 1 and grow after a period of time until measuring plants Fischer grass seedling, guiding function due to growing space restriction, Action of Gravity Field and cone, root system of plant is oliquely downward grown along cone, through the agar hole on growth case 1 outer wall, from growth case 1, enter into collecting box 2, grow after three weeks, can carry out the collection of root exudates.Before collection, to use the CaCl of 0.5mM
2solution is fully cleaned collecting box 2.Open after switch 6, eluent gravitate flows into resin column 8, and root exudates is by resin adsorption.Under the effect of ebullator, nutrient solution recycling elution root system, root exudates can be in resin column 8 places enrichment.The continuous collection that can complete a root exudates for five times.Last available 8N HCOOH solution and 1N NH
4oH eluant solution resin, collects root exudates, obtains root exudates solution.
Claims (5)
1. a circular conical surface percolation type is collected the device of secretion from root system of plant in pot, it is characterized in that, comprise growth case (1) and collecting box (2), in growth case (1), place for examination soil (3), on the outer wall of described growth case (1), have hole (4), and fill with agar, described growth case (1) and collecting box (2) junction, lower end are provided with collection hole (11), described collection hole (11) lower end connects nylon wire (7), described nylon wire (7) lower end connecting duct (9), conduit (9) connects respectively rubber stopper with holes (5) from top to bottom, switch (6) and resin column (8), the conduit (9) of resin column (8) lower end takes back collecting box (2) top placement silica sand (10) and locates.
2. circular conical surface percolation type according to claim 1 is collected the device of secretion from root system of plant in pot, it is characterized in that, described growth case (1) and collecting box (2) are cylindrical, described growth case (1) outer wall is certainly apart from 100mm place, top to having hole (4), aperture 6mm, pitch of holes is 4mm.
3. circular conical surface percolation type according to claim 1 is collected the device of secretion from root system of plant in pot, it is characterized in that, described silica sand (10) diameter is 2 ~ 3mm.
4. a circular conical surface percolation type is collected the method for secretion from root system of plant in pot, it is characterized in that, comprise the steps: to treat measuring plants growing for examination soil (3) is middle in growth case (1), due to growing space restriction and Action of Gravity Field, root system of plant to be measured is through the agar in growth case (1) outer wall hole (4), enter in the collecting box (2) that silica sand (10) are housed, until collecting box (2) accumulation, have after certain root system of plant, carry out the collection of root exudates; Then with eluent, the root system being grown in silica sand (10) is carried out to wash-out, flow out under solution seepage flow in resin column (8), and by resin adsorption; Last wash-out resin, obtains root exudates solution.
5. circular conical surface percolation type according to claim 4 is collected the method for secretion from root system of plant in pot, it is characterized in that, before carrying out the collection of root exudates, need first with the calcium liquid of 0.5mM, clean collecting box (2).
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| CN201410223952.XA CN104006988A (en) | 2014-05-26 | 2014-05-26 | Conical surface percolation type device and method for collecting root exudates of soil cultivation plants |
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| CN201410223952.XA CN104006988A (en) | 2014-05-26 | 2014-05-26 | Conical surface percolation type device and method for collecting root exudates of soil cultivation plants |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105115780A (en) * | 2015-08-24 | 2015-12-02 | 成都中医药大学 | Plant rhizosphere soil and rhizosphere exudate collecting device and method |
| CN107976330A (en) * | 2017-11-21 | 2018-05-01 | 东北师范大学 | The collection device in situ and method of secretion from root system of plant in pot and rhizosphere soil |
| CN110672351A (en) * | 2019-11-08 | 2020-01-10 | 华南农业大学 | Device and method for collecting root exudates of gramineous plants |
| CN111011029A (en) * | 2019-12-31 | 2020-04-17 | 中国科学院成都生物研究所 | Plant whole life cycle rhizosphere information acquisition method and system |
| CN114112480A (en) * | 2021-12-17 | 2022-03-01 | 贵州省生物研究所 | Device and method for collecting pepper rhizosphere secretion |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105115780A (en) * | 2015-08-24 | 2015-12-02 | 成都中医药大学 | Plant rhizosphere soil and rhizosphere exudate collecting device and method |
| CN105115780B (en) * | 2015-08-24 | 2018-09-28 | 成都中医药大学 | Plant rhizosphere soil and root rhizosphere secreta collection device and collection method |
| CN107976330A (en) * | 2017-11-21 | 2018-05-01 | 东北师范大学 | The collection device in situ and method of secretion from root system of plant in pot and rhizosphere soil |
| CN110672351A (en) * | 2019-11-08 | 2020-01-10 | 华南农业大学 | Device and method for collecting root exudates of gramineous plants |
| CN111011029A (en) * | 2019-12-31 | 2020-04-17 | 中国科学院成都生物研究所 | Plant whole life cycle rhizosphere information acquisition method and system |
| CN111011029B (en) * | 2019-12-31 | 2022-10-14 | 中国科学院成都生物研究所 | Plant whole life cycle rhizosphere information acquisition method and system |
| CN114112480A (en) * | 2021-12-17 | 2022-03-01 | 贵州省生物研究所 | Device and method for collecting pepper rhizosphere secretion |
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Application publication date: 20140827 |