CN209961502U - Plant root system secretion collection device - Google Patents
Plant root system secretion collection device Download PDFInfo
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- CN209961502U CN209961502U CN201920662610.6U CN201920662610U CN209961502U CN 209961502 U CN209961502 U CN 209961502U CN 201920662610 U CN201920662610 U CN 201920662610U CN 209961502 U CN209961502 U CN 209961502U
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Abstract
The utility model discloses a plant roots secretion collection device, include: the device comprises a test plant, a soil layer for plant growth, a secretion collecting box wrapping the soil layer, a detachable isolation assembly arranged between the soil layer and the secretion collecting box, and a resin enrichment pipe connected below the secretion collecting box; the utility model discloses a soil horizon can keep the situation of plant roots free growth in soil with the cooperation that can dismantle isolation component, deuterogamies secretion collecting box, resin enrichment pipe and can realize the collection of root system secretion well.
Description
Technical Field
The utility model relates to a plant research experimental apparatus field, especially a plant roots secretion collection device.
Background
As to the solution collection method of the root exudates, a great deal of research work has been reported, namely, the root system is immersed in the aqueous solution or the nutrient solution to be cultured for a period of time, and the root system culture solution is collected, namely, the root exudates.
In the prior art, a system for collecting the secretion of the circulating water root system is designed, so that a plant root system grows in nutrient solution, the nutrient solution is periodically circulated, the root system is eluted, the secretion flows along with water, the secretion is continuously enriched in XAD resin, and then the resin is collected and eluted, so that the secretion of the root system is obtained. Based on the device, a novel continuous root exudate collecting device is also designed, and the principle of the device is the same as that of the device designed in the previous step. The difference is that the plant root system grows in a mixed matrix of river sand, quartz sand and cobblestones, the water is used for periodically and circularly eluting the root system, the root system eluent circulates and is enriched in a resin tube, and the resin is eluted to obtain root system secretion. The method can be used for quickly and continuously collecting the root exudates, is simple and convenient, and has certain practicability, but the root exudates are collected when the plant root system is under a flooding condition, the growth resistance of the root system is small, and the method is greatly different from the real root exudates under the soil cultivation condition.
A root canal with a vacuum micropipette is also designed for in-situ extraction of rhizosphere soil solution, similar to the novel in-situ soil solution collecting device introduced previously, the rhizosphere soil solution can be collected, the method is convenient to sample, and the sampling space is small; but has the defects of easy damage of root systems, poor repeatability and the like in sampling.
A filter paper collection method of root exudates under the condition of soil culture is discussed, one side surface of the root system growth is covered by a movable organic glass plate, and the root system can grow by abutting against the wall of the organic glass plate. The organic glass plate is opened periodically, and the growth of the root system can be observed. According to the root growth position, covering a chromatographic adsorption filter paper, adsorbing the root secretion by the filter paper, harvesting periodically, and eluting the filter paper to obtain the root secretion. The method can extract root secretion, and has flexible sampling position and no damage to root system. However, the root box is frequently opened in the operation process, and the growth of the root system is disturbed.
Methods for collecting root exudates by agar membrane method and chromatography have also been tried. Researches show that the agar membrane has high adsorption capacity to root exudates; compared with a solution collection method, the method can reflect the natural secretion condition of the plant root system, but has the defects that an agar membrane or chromatographic filter paper is easy to lose water on the surface of the root system, the adsorption capacity of the agar membrane or the filter paper is small, and the like.
Some designs an inclined growth box body, and the root system penetrates through an agar layer to enter a collection box through gravity and inclined directional guidance to collect root system secretion, but the method changes and blocks the growth direction of the plant root system and cannot truly reflect the free growth condition of the root system;
the market needs a collecting device which can keep the free growth condition of the plant root system in the soil and realize the collection of root system secretion; the utility model provides such a problem.
SUMMERY OF THE UTILITY MODEL
For solving prior art's is not enough, the utility model aims to provide a plant roots secretion collection device, the soil horizon can keep the situation that plant roots freely grows in soil with the cooperation that can dismantle isolation component, deuterogamies the collection that secretion collecting box, resin enrichment pipe can realize the root system secretion well.
In order to achieve the above object, the utility model adopts the following technical scheme:
a plant root exudate collection device, comprising: the device comprises a test plant, a soil layer for plant growth, a secretion collecting box wrapping the soil layer, a detachable isolation assembly arranged between the soil layer and the secretion collecting box, and a resin enrichment pipe connected below the secretion collecting box.
Aforementioned a plant roots secretion collection device, secretion collecting box constitute has: the container loads in the container and the parcel can dismantle the secretion collection layer of isolation component, cover the top opening of container and can dismantle the cover strip between the isolation component, set up in the filter screen of the bottom opening of container.
In the aforementioned plant root exudate collection device, the exudate collection layer is a quartz sand layer.
In the aforementioned plant root exudate collection device, the cover strip is a sponge strip.
In the aforementioned plant root exudate collection device, the filter screen is a nylon screen.
Aforementioned a plant roots secretion collection device can dismantle isolation component and constitute and have: the baffle is arranged between the soil layer and the secretion collecting box, and the agar layer is filled between the baffles.
In the aforementioned plant root exudate collection device, the baffle is a transparent plastic diaphragm.
Aforementioned a plant roots secretion collection device, resin enrichment pipe is constituteed and is had: a containing tube connected to the bottom opening of the secretion collecting box, resin filled in the containing tube, and a switch connected between the containing tube and the secretion collecting box.
The aforementioned plant root system secretion collection device still includes: a duct blowing assembly connected to the secretion collection tank and the resin enrichment tube.
Aforementioned a plant roots secretion collection device, pipe air blowing subassembly is constituteed and is had: the air blowing port, a first conduit connected between the air blowing port and the upper end of the secretion collecting box, a second conduit connected between the air blowing port and the bottom end of the resin enrichment pipe, and a rubber interface connected among the air blowing port, the first conduit and the second conduit.
The utility model discloses an useful part lies in:
the utility model can lead the plant root system to grow freely in the soil through the matching of the soil layer and the detachable isolation component, thereby truly reflecting the free growth condition of the root system;
the secretion collecting box and the resin enrichment pipe can well realize the collection of root system secretions;
the pipe subassembly of blowing promotes the liquid circulation and flows constantly and elute the root exudates in the collecting box, makes the root exudates enrichment in the resin, improves the collection rate.
Drawings
Fig. 1 is a schematic structural diagram of an embodiment of the present invention.
The meaning of the reference symbols in the figures:
1 supply test plant, 2 soil horizon, 3 agar layer, 4 secretion collecting layer, 5 baffles, 6 filter screens, 7 switches, 8 resin, 9 containers, 10 cover strips, 11 plant fixed bolsters, 12 device fixed bolsters, 13 glass pipes, 14 gas blowing openings, 15 rubber connectors.
Detailed Description
The present invention will be described in detail with reference to the accompanying drawings and specific embodiments.
A plant root exudate collection device, comprising: the device comprises a test plant 1, a soil layer 2 for plant growth, a secretion collecting box wrapping the soil layer 2, a detachable isolation assembly arranged between the soil layer 2 and the secretion collecting box, and a resin 8 enrichment pipe connected below the secretion collecting box. The test plant 1 is fixed and supported by the plant fixing bracket 11.
The secretion collecting box comprises: the secretion collecting layer 4 is loaded in the container 9 and wraps the detachable isolation component, the covering strip 10 covers between the top end opening of the container 9 and the detachable isolation component, and the filter screen 6 is arranged at the bottom end opening of the container 9. The container 9 is supported and fixed by a device fixing bracket 12 as shown in fig. 1. As an example, the container 9 is a glass container 9, the secretion collecting layer 4 is a quartz sand layer, the cover strip 10 is a sponge strip, and the filter screen 6 is a nylon screen, and the material is not limited.
The detachable isolation component comprises: a baffle 5 arranged between the soil layer 2 and the secretion collecting box, and an agar layer 3 filled between the baffle 5. As an example, the baffle 5 is a transparent plastic membrane, preferably a transparent plastic membrane having a thickness of 2 mm. Firstly arranging two baffles 5, filling agar between the two baffles 5, after the agar is solidified, extracting the plastic partition plate, enabling the root system to grow and penetrate the agar to enter the collection box, adsorbing and gathering the secretion in the quartz sand after the root system grows to a certain biomass, and leaching the quartz sand to obtain the root system secretion.
The resin 8 enrichment pipe comprises the following components: a holding tube connected to the bottom end opening of the secretion collecting box, a resin 8 filled in the holding tube, and a switch 7 connected between the holding tube and the secretion collecting box. As an example, XAD-4 resin 8 is used as resin 8.
A plant root exudate collection device, still includes: a duct blowing assembly connected to the secretion collection tank and the resin 8 enrichment tube; the catheter blowing component comprises: the air blowing port 14 is connected with a first guide pipe between the air blowing port 14 and the upper end of the secretion collecting box, a second guide pipe between the air blowing port 14 and the bottom end of the resin 8 enrichment pipe, and a rubber interface 15 between the air blowing port 14, the first guide pipe and the second guide pipe; the number and shape of the first and second conduits are not limited, and as an example, the material is selected from the glass tube 13; as an embodiment, the air blowing port 14 is connected with an air blowing pump, or other air blowing devices, and the air pushes the liquid to circularly flow so as to continuously elute the root exudates in the collecting box, so that the root exudates are enriched in the resin 8, and the collecting rate is improved.
The specific using steps are illustrated as follows by taking the collection of the organic acid secretion of the pinus massoniana seedling root system as an example: let the plant grow in soil horizon 2 earlier, furthest does not influence vegetation, through extracting plastics baffle 5, makes the root system of growing pass through agar, gets into root exudates collecting box, in the quartz sand layer. After the root system accumulates a certain biomass in the collecting box, the gas generated by the blowing pump pushes the liquid to circularly flow, and the root secretion in the collecting box is continuously eluted, so that the root secretion is enriched in the resin 8; and finally, taking out the resin 8 for elution to obtain the root exudates.
The structural design of the utility model enables the plant root system to grow freely in the soil, thereby achieving the simulation effect of the growing environment; the collecting of root exudates is convenient to use, the exudates are collected comprehensively, and the collecting rate is high.
The foregoing illustrates and describes the principles, general features, and advantages of the present invention. It should be understood by those skilled in the art that the above embodiments do not limit the present invention in any way, and all technical solutions obtained by adopting equivalent replacement or equivalent transformation fall within the protection scope of the present invention.
Claims (10)
1. A plant root system secretion collection device which characterized in that includes: the device comprises a test plant, a soil layer for plant growth, a secretion collecting box wrapping the soil layer, a detachable isolation assembly arranged between the soil layer and the secretion collecting box, and a resin enrichment pipe connected to the lower part of the secretion collecting box.
2. The apparatus for collecting the secretion of the root system of a plant as claimed in claim 1, wherein said secretion collecting box is composed of: the container loads in the container and the parcel can dismantle the secretion collection layer of isolation component, cover the top opening of container and can dismantle the cover strip between the isolation component, set up in the filter screen of the bottom opening of container.
3. The device for collecting the exudate from the root system of a plant of claim 2, wherein the exudate collecting layer is a quartz sand layer.
4. The device for collecting root exudates of plants according to claim 2, wherein said covering strip is a sponge strip.
5. The device for collecting the secretion of the root system of a plant as claimed in claim 2, wherein the filter screen is a nylon screen.
6. The apparatus according to claim 1, wherein the removable spacer assembly comprises: the baffle is arranged between the soil layer and the secretion collecting box, and the agar layer is filled between the baffles.
7. The device for collecting root exudates of plants as claimed in claim 6, wherein said baffle is a transparent plastic membrane.
8. The plant root exudate collection device of claim 1, wherein the resin enrichment tube is comprised of: a containing tube connected to the bottom opening of the secretion collecting box, resin filled in the containing tube, and a switch connected between the containing tube and the secretion collecting box.
9. The plant root exudate collection device of claim 1, further comprising: a duct blowing assembly connected to the secretion collection tank and the resin enrichment tube.
10. The apparatus for collecting the secretion of the root system of a plant as claimed in claim 9, wherein said duct blowing assembly comprises: the air blowing port, the first guide pipe connected between the air blowing port and the upper end of the secretion collecting box, the second guide pipe connected between the air blowing port and the bottom end of the resin enrichment pipe, and the rubber interface connected among the air blowing port, the first guide pipe and the second guide pipe.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920662610.6U CN209961502U (en) | 2019-05-09 | 2019-05-09 | Plant root system secretion collection device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920662610.6U CN209961502U (en) | 2019-05-09 | 2019-05-09 | Plant root system secretion collection device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN209961502U true CN209961502U (en) | 2020-01-17 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201920662610.6U Expired - Fee Related CN209961502U (en) | 2019-05-09 | 2019-05-09 | Plant root system secretion collection device |
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| CN (1) | CN209961502U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115219656A (en) * | 2022-06-23 | 2022-10-21 | 海南大学 | Device for marking in-situ collection of root exudates and rhizosphere soil |
-
2019
- 2019-05-09 CN CN201920662610.6U patent/CN209961502U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115219656A (en) * | 2022-06-23 | 2022-10-21 | 海南大学 | Device for marking in-situ collection of root exudates and rhizosphere soil |
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| GR01 | Patent grant | ||
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| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20200117 Termination date: 20210509 |