CN103494038B - Anti-white spot syndrome virus Chinese medicine immunity enhancer of a kind of Procambius clarkii and its preparation method and application - Google Patents
Anti-white spot syndrome virus Chinese medicine immunity enhancer of a kind of Procambius clarkii and its preparation method and application Download PDFInfo
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Abstract
The invention discloses anti-white spot syndrome virus Chinese medicine immunity enhancer of a kind of Procambius clarkii and its preparation method and application.Get rheum officinale, barrenwort, the Radix Astragali, Radix Isatidis; Fully mix according to proportioning after above-mentioned each component is pulverized, make powdery immunopotentiator.Raw materials enjoy stable sources of the present invention, preparation is simple, draws materials extensively, with low cost; And from the immune mechanism of shellfish, supplement Chinese herbal medicine, during use, immunity of organism and oxidation resistance can be improved, improve the infection ability of the anti-WSSV of Procambius clarkii, and can promote that shrimp grows.
Description
Technical field
The invention belongs to aquaculture field, relate to anti-white spot syndrome virus Chinese medicine immunity enhancer of a kind of Procambius clarkii and its preparation method and application.
Background technology
Procambius clarkii (Procambarus clarkii) is commonly called as " cray ", be subordinate to Crustachia (Cmstacea), Decapoda (Decapoda), Astacidae (Cambaridae), former Astacus (Procambarus), because of its delicious meat, be nutritiously subject to consumers, it is topmost crayfish cultivation kind in the world today, its output accounts for 70% ~ 80%, Ye Shi China of whole crayfish output economic aquaculture object important at present.In recent years, Procambius clarkii cultivation scale expands year by year, and according to statistics, only the water surface of Jingzhou Prefecture of Hubei Province cultivation Procambius clarkii just reaches tens thousand of hectare.But along with propagating the rapid expansion of area artificially, the generation of disease is also more and more frequent and serious.The domestic disease such as spiral shell substance disease, fin rot in succession reporting Procambius clarkii, these diseases bring heavy economic losses to Procambius clarkii culturist, wherein white spot syndrome virus (White SpotSyndrome Virus, WSSV) be a kind of without Viruses With Inclusion Body, geographical distribution is wide, Epidemic Scope is large, and infectiousness is strong, there is no effective therapy approach at present.In recent years, white spot syndrome virus comes into vogue in Procambius clarkii cultivation, and becomes the important disease in Procambius clarkii cultivation, carrys out massive losses to cultivation industrial belt.
Treat traditionally and prevent Procambius clarkii disease mainly to rely on antibiotic, chemicals etc., but these drug abuses easily cause environmental pollution, medicament residue, the drug resistance of pathogenic microorganism, many negative effects such as immunosupress and destruction body microecological balance, seriously annoying the cultivation of Procambius clarkii.Therefore, the immunity improving animal self is very important to solution the farming disease harms problem.Natural Chinese medicinal herb is taken from natural animal-plant product, maintain its composition, the nature of structure and biologically active, as feed addictive, not only there are extra-nutrition, growth promoting effects, build up health, improve the effect of premunition, compared with adding medicament with chemistry, also there is the features such as low toxicity, low-residual, drug resistance are not remarkable.Therefore Chinese herb feed additive becomes the additive of new generation of substitute antibiotics and chemical synthetic drug day by day.Rheum officinale, barrenwort, the Radix Astragali and Radix Isatidis are China's traditional Chinese medicine materials, have antiviral, anti-oxidant, effect such as adjustment humoral immunity of organism, the activate immunity factor etc., especially with rheum officinale best results.But it remains the weak point in single use, in raising shellfish immunity, anti-WSSV aspect DeGrain.
Shellfish is without specific immune system, based on nospecific immunity, lysozyme (LZM), superoxide dismutase (SOD), CER (CP) and peroxidase (POD) etc. are important immune factors, resist the invasion and attack of pathogen in a different manner.Therefore, many scholars evaluate shellfish immunity with above index.
More existing reports about a small amount of use in aquaculture of rheum officinale, barrenwort, the Radix Astragali and Radix Isatidis at present, as CN102389499A discloses a kind of Chinese medicine extract for the control of aquaculture Disease, it is mainly made up of the raw material of following weight portion: rheum officinale 40 ~ 60 parts, the Radix Astragali 20 ~ 40 parts, Radix Codonopsis 15 ~ 25 parts, granatum 20 ~ 25 parts, folium isatidis 15 ~ 25 parts, Radix Isatidis 20 ~ 30 parts, barrenwort 15 ~ 25 parts, windproof 20 ~ 30 parts, the root of purple-flowered peucedanum 15 ~ 25 parts, 10 ~ 20 parts, Radix Glycyrrhizae and peppermint 20 ~ 30 parts.But this Chinese medicine extract is mainly effective to the immunity of organisms and resistance against diseases that improve fish, to shrimps, particularly Procambius clarkii then DeGrain.At present about the compatibility of rheum officinale, barrenwort, the Radix Astragali and Radix Isatidis uses and have not been reported Procambius clarkii impact.Therefore, by to the mensuration of Serum of Procambarus clarkii and the research of death condition after infecting WSSV, the Chinese herbal medicine immunomodulator of screening growth promoting effects and adjustment Procambius clarkii non-specific immune function, may further be Procambius clarkii immune response study mechanism and improve its resistance against diseases scientific basis is provided, to Procambius clarkii healthy aquaculture and sustainable development significant.
Summary of the invention
The object of the invention is the above-mentioned deficiency for prior art, provide a kind of Procambius clarkii anti-white spot syndrome virus Chinese medicine immunity enhancer.
Another object of the present invention is to provide the preparation method of the anti-white spot syndrome virus Chinese medicine immunity enhancer of this Procambius clarkii.
Another object of the present invention is to provide the application of the anti-white spot syndrome virus Chinese medicine immunity enhancer of this Procambius clarkii.
Object of the present invention realizes by following technical scheme:
The anti-white spot syndrome virus herbal composite of a kind of Procambius clarkii, composed of the following components: rheum officinale 25 ~ 45 weight portion, barrenwort 20 ~ 35 weight portion, the Radix Astragali 15 ~ 30 weight portion, Radix Isatidis 12 ~ 32 weight portion.
Described herbal composite is preferably composed of the following components: rheum officinale 30 ~ 38 weight portion, barrenwort 28 ~ 35 weight portion, the Radix Astragali 15 ~ 25 weight portion, Radix Isatidis 15 ~ 25 weight portion.
Herbal composite of the present invention is in the application preparing the anti-white spot syndrome virus of Procambius clarkii and/or improve in the preparation of immunity.
The anti-white spot syndrome virus Chinese medicine immunity enhancer of a kind of Procambius clarkii, containing herbal composite of the present invention.
Described immunopotentiator prepares preferably by following methods: by first for each for composition of the present invention component coarse crushing, pulverize through micronizer again, after crossing 120 object screen clothes, fully mix, namely make described immunopotentiator in mixer.
The preparation method of the anti-white spot syndrome virus Chinese medicine immunity enhancer of Procambius clarkii of the present invention, by first for each for herbal composite of the present invention component coarse crushing, pulverize through micronizer again, after crossing 120 object screen clothes, fully mix in mixer, namely make described immunopotentiator.
The application of Procambius clarkii of the present invention anti-white spot syndrome virus Chinese medicine immunity enhancer in the anti-white spot syndrome virus feed of preparation Procambius clarkii.
Described Procambius clarkii anti-white spot syndrome virus feed adds described Chinese medicine immunity enhancer in conventional freshwater crayfish feed stuff gained according to the amount of 1wt% ~ 1.5wt%.
Beneficial effect:
The present invention is directed to the characteristic of Procambius clarkii, from the immune mechanism of shellfish, rheum officinale, barrenwort, the Radix Astragali and Radix Isatidis is filtered out from Chinese herbal medicine, and it is composite according to specific proportioning, and one is well make use of rheum officinale and the Radix Astragali " mutual-assistance relation "; Two are rheum officinales, barrenwort and the Radix Astragali all have immunity moderation and anti-inflammatory activity, can bidirectional modulation immune response, the generation of the various factor in inflammatory reaction process; Three is with Radix Astragali tonifying middle-Jiao and Qi, and barrenwort immunity moderation is to hold up, and Radix Isatidis is clearing heat and detoxicating with eliminating evil, and three medicines share, and plays strengthening vital QI to eliminate pathogenic factors, clearing heat and detoxicating merit altogether.Four kinds of medicines are composite, overcome the weak point of single medicine, obtain anti-white spot syndrome virus herbal composite, and prepare the anti-white spot syndrome virus Chinese medicine immunity enhancer of Procambius clarkii on this basis.This immunopotentiator directly can remove the free radical in Procambius clarkii body, eliminate the negative effect of radical pair body tissue organ normal function, accelerate to repair destroyed immune system, the immunity of body, oxidation resistance and opposing WSSV infection ability can be improved, promote Procambius clarkii body growth, improve survival rate.
Raw materials enjoy stable sources of the present invention, preparation is simple, draws materials extensively, with low cost.
Detailed description of the invention
Embodiment 1
Get rheum officinale 30 weight portion, barrenwort 30 weight portion, the Radix Astragali 20 weight portion, Radix Isatidis 20 weight portion; By first for above-mentioned each component coarse crushing, pulverize through micronizer again, after crossing 120 object screen clothes, fully mix in mixer, make immunopotentiator.
Embodiment 2
Growth test: Procambius clarkii is provided by Jiangsu Fresh Water Aquatic Product Inst. Lu Kou base.The shrimp that test selects health, specification, weight basically identical, individual just counterpoise is about 7.5g.Random packet, be provided with control group A (basal diet of throwing something and feeding), control group B(add 1.0% rheum officinale in basal diet), control group C(adds 0.5% rheum officinale and 0.5% barrenwort in basal diet) with test group (adding Chinese medicine immunity enhancer prepared by 1.0% embodiment 1 in basal diet), often group establishes 3 parallel tests, every parallel group 50 tail test shrimp, totally 6 cement pits (2.50m × 2.5m × 0.8m).Shrimp is thrown something and fed after taming 7 days control group basal diet and 1.0% Chinese medicine immunity enhancer 60 days, measures its index such as growth, immunity.
Table 1 basal diet formula
Note: compound premix (every kg feed): vitamin e1 50mg, vitamin K5 0mg, vitamin B1 80mg, vitamin B2 50mg, nicotinic acid 150mg, pantothenic acid 150mg, vitamin B6 50mg, biotin 1mg, vitamin B12 0.02mg, folic acid 10mg, vitamin C 300IU, retinol1 0000IU, calciferol 000IU. copper sulphate 2.0g, ferric sulfate 25g, zinc sulfate 22g, manganese sulfate 7g, sodium selenite 0.04g, KI 0.026g, cobalt chloride 0.1g.
Table 2
* heavy-average just body weight of the average end-body of average growth rate %=()/average just body weight * 100
Result shows: compared with control group A, test group significantly improves: rate of body weight gain 27.55%, lysozyme (LZM) vigor 96.38%, albumin (ALB) content 66.92%, CER (CP) vigor 27.36%, total protein (TP) content 8.50%, total number born (SOD) vigor 23.00%, nitric oxide (NO) content 57.55%, peroxidase (POD) vigor 12.37%, substantial reduction in triglycerides (TG) content 35.37% (P<0.05); Compared with control group B, test group significantly improves: rate of body weight gain 25.06%, albumin (ALB) content 56.01%, nitric oxide (NO) content 17.72%, peroxidase (POD) vigor 8.50% (P<0.05); Compared with control group C, test group significantly improves: albumin (ALB) content 18.12%, nitric oxide (NO) content 25.38%, peroxidase (POD) vigor 9.74% (P<0.05).Therefore, immunopotentiator of the present invention can significantly improve the immunity of shrimp body and oxidation resistance than each control group, promotes shrimp bulk-growth.
Challenge test: growth test is after 60 days, fasting 24 hours, select the consistent shrimp of specification more often to organize 30 tails and carry out WSSV infection experiment, observe the physiological activity after shrimp injection WSSV virus (attacking poison), and add up control group A (basal diet of throwing something and feeding), control group B(adds 1.0% rheum officinale in basal diet), control group C(adds 0.5% rheum officinale and 0.5% barrenwort in basal diet) with test group (adding Chinese medicine immunity enhancer prepared by 1.0% embodiment 1 in basal diet), the cumulative mortality of four groups of shrimps 0h, 12h, 24h, 36h, 48h, 60h.
Table 3
Result shows: after Procambius clarkii infects WSSV, and the vigor of shrimp starts the phenomenons such as decline, to external world respond weaken; Each timing statistics point, the results are shown in Table 3.From table 3, compared with control group A, the 1.0% Chinese medicine immunity enhancer death rate significantly reduces 27.27%, 53.86% (P<0.05) at 48h, 60h; Compared with control group B, the 1.0% Chinese medicine immunity enhancer death rate significantly reduces 18.18%, 15.38% (P<0.05) at 48h, 60h; Compared with control group C, the 1.0% Chinese medicine immunity enhancer death rate significantly reduces 18.18%, 7.69% (P<0.05) at 48h, 60h.Therefore, this immunopotentiator can improve Procambius clarkii opposing WSSV infection ability, reduces the death caused by infection WSSV.
Embodiment 3
Get rheum officinale 30 weight portion, barrenwort 25 weight portion, the Radix Astragali 15 weight portion, Radix Isatidis 15 weight portion; By first for above-mentioned each component coarse crushing, pulverize through micronizer again, after crossing 120 object screen clothes, fully mix in mixer, make immunopotentiator.
Embodiment 4
Get rheum officinale 33 weight portion, barrenwort 30 weight portion, the Radix Astragali 19 weight portion, Radix Isatidis 18 weight portion; By first for above-mentioned each component coarse crushing, pulverize through micronizer again, after crossing 120 object screen clothes, fully mix in mixer, make immunopotentiator.
Embodiment 5
Get rheum officinale 38 weight portion, barrenwort 28 weight portion, the Radix Astragali 25 weight portion, Radix Isatidis 25 weight portion; By first for above-mentioned each component coarse crushing, pulverize through micronizer again, after crossing 120 object screen clothes, fully mix in mixer, make immunopotentiator.
Embodiment 6
Get rheum officinale 30 weight portion, barrenwort 25 weight portion, the Radix Astragali 15 weight portion, Radix Isatidis 15 weight portion; By first for above-mentioned each component coarse crushing, pulverize through micronizer again, after crossing 120 object screen clothes, fully mix in mixer, make immunopotentiator.
Embodiment 7
Get rheum officinale 37 weight portion, barrenwort 32 weight portion, the Radix Astragali 23 weight portion, Radix Isatidis 23 weight portion; By first for above-mentioned each component coarse crushing, pulverize through micronizer again, after crossing 120 object screen clothes, fully mix in mixer, make immunopotentiator.
Embodiment 8
Get rheum officinale 25 weight portion, barrenwort 24 weight portion, the Radix Astragali 17 weight portion, Radix Isatidis 15 weight portion; By first for above-mentioned each component coarse crushing, pulverize through micronizer again, after crossing 120 object screen clothes, fully mix in mixer, make immunopotentiator.
Embodiment 9
Get rheum officinale 43 weight portion, barrenwort 32 weight portion, the Radix Astragali 30 weight portion, Radix Isatidis 28 weight portion; By first for above-mentioned each component coarse crushing, pulverize through micronizer again, after crossing 120 object screen clothes, fully mix in mixer, make immunopotentiator.
Embodiment 10
According to the growth test in embodiment 2 and challenge test method, measure index and infection WSSV48h and the 60h cumulative mortality such as Procambius clarkii growth, immunity of throwing something and feeding containing the immunopotentiator of preparation in embodiment 3 ~ 9 respectively.Control group is thrown something and fed basal diet, and each test group adds Chinese medicine immunity enhancer prepared by 1.0% embodiment 3 ~ 9 respectively in basal diet.The results are shown in Table 4 and table 5.
Table 4
* heavy-average just body weight of the average end-body of average growth rate %=()/average just body weight * 100
Table 5
Comparative example 1
Record according to CN102389499A embodiment 3 and prepare Chinese medicine extract 3.
Contrast on effect example
Growth test: Procambius clarkii is provided by Jiangsu Fresh Water Aquatic Product Inst. Lu Kou base.The shrimp that test selects health, specification, weight basically identical, individual just counterpoise is about 7.5g.Random packet, be provided with control group: in basal diet, add Chinese medicine extract 3 prepared by 1.0% comparative example 1, basal diet formula is shown in embodiment 2, test group: in basal diet, add Chinese medicine immunity enhancer prepared by 1.0% embodiment 1, basal diet formula is shown in embodiment 1; Parallel group 50 tail test shrimp, totally 6 cement pits (2.50m × 2.5m × 0.8m).Shrimp is thrown something and fed after taming 7 days control group and test group feed 60 days, and measure its index such as growth, immunity, result data is in table 6.Visible immunopotentiator of the present invention can significantly improve the immunity of shrimp body and oxidation resistance than the Chinese medicine extract of CN102389499A, promotes shrimp bulk-growth.
Table 6
* heavy-average just body weight of the average end-body of average growth rate %=()/average just body weight * 100
Challenge test: growth test is after 60 days, fasting 24 hours, select the consistent shrimp of specification more often to organize 30 tails and carry out WSSV infection experiment, observe the physiological activity after shrimp injection WSSV virus (attacking poison), and add up control group (adding Chinese medicine extract 3 prepared by 1.0% comparative example 1 in basal diet) and test group (adding Chinese medicine immunity enhancer prepared by 1.0% embodiment 1 in basal diet), the cumulative mortality of two groups of shrimps 0h, 12h, 24h, 36h, 48h, 60h, the results are shown in Table 7.Have result visible, immunopotentiator of the present invention can significantly improve Procambius clarkii opposing WSSV infection ability than the Chinese medicine extract of CN102389499A, reduces the death caused by infection WSSV.
Table 7
Claims (4)
1. the anti-white spot syndrome virus Chinese medicine immunity enhancer of Procambius clarkii, is characterized in that by rheum officinale 30 ~ 38 weight portion, barrenwort 28 ~ 35 weight portion, the Radix Astragali 15 ~ 25 weight portion, and Radix Isatidis 15 ~ 25 weight portion forms; Described immunopotentiator prepares by the following method: by first for each component coarse crushing, pulverize through micronizer again, after crossing 120 object screen clothes, fully mix, namely make described immunopotentiator in mixer.
2. the preparation method of the anti-white spot syndrome virus Chinese medicine immunity enhancer of Procambius clarkii according to claim 1, it is characterized in that rheum officinale 30 ~ 38 weight portion, barrenwort 28 ~ 35 weight portion, the Radix Astragali 15 ~ 25 weight portion, the first coarse crushing of Radix Isatidis 15 ~ 25 weight portion, to pulverize through micronizer again, after crossing 120 object screen clothes, fully mix in mixer, namely make described immunopotentiator.
3. the application of Procambius clarkii according to claim 1 anti-white spot syndrome virus Chinese medicine immunity enhancer in the anti-white spot syndrome virus feed of preparation Procambius clarkii.
4. application according to claim 3, is characterized in that described Procambius clarkii anti-white spot syndrome virus feed adds Chinese medicine immunity enhancer according to claim 1 in conventional freshwater crayfish feed stuff gained according to the amount of 1wt% ~ 1.5wt%.
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| CN104082631B (en) * | 2014-07-11 | 2016-01-20 | 中国农业科学院兰州畜牧与兽药研究所 | For preventing and treating the feed of giant freshwater prawn white body disease, Chinese medicine composition and preparation method |
| CN106309662A (en) * | 2015-06-24 | 2017-01-11 | 罗永辉 | Medicine for treating pullorum disease |
| CN106581041A (en) * | 2016-12-01 | 2017-04-26 | 兴化市恒威生物技术有限公司 | Immunopotentiator for preventing and treating crayfish white spot syndrome virus (WSSV) and use method thereof |
| CN106666223A (en) * | 2016-12-05 | 2017-05-17 | 浙江海洋大学 | Chinese herbal medicine feed additive capable of improving immunity of marine culture shrimp and application thereof |
| CN106616030A (en) * | 2016-12-08 | 2017-05-10 | 安徽省农业科学院水产研究所 | Compound Chinese herbal medicinal preparation for preventing and curing white spot disease of procambarus clarkia and method thereof |
| CN107318727B (en) * | 2017-07-21 | 2021-12-17 | 句容市润泰生物科技有限公司 | Comprehensive control method for crayfish diseases |
| CN107224509A (en) * | 2017-07-21 | 2017-10-03 | 句容市润泰生物科技有限公司 | Chinese medicine composition of anti-cray guttate morphea syndrome and its preparation method and application |
| CN109699847A (en) * | 2017-10-25 | 2019-05-03 | 湖北汇洋生物科技饲料有限公司 | A kind of lobster feed and preparation method thereof |
| CN110540996B (en) * | 2019-08-29 | 2022-01-28 | 华中农业大学 | Procambarus clarkii i-type lysozyme gLysi2 gene, gLysi2 protein coded by same and application thereof |
| CN110731427B (en) * | 2019-10-11 | 2023-05-12 | 南京农业大学 | Gonadotrophin additive for fishing and its preparation method and application |
| CN111642655A (en) * | 2020-06-22 | 2020-09-11 | 江苏海洋大学 | Method for developing compound Chinese herbal medicine feed additive for procambarus clarkia |
| CN114504003B (en) * | 2022-02-08 | 2023-04-18 | 浙江省淡水水产研究所 | Disinfectant for in-vitro hatching of procambarus clarkia embryos and preparation method and application thereof |
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