CN103444610A - Artificial propagation method for acrossocheilus yunnanensis - Google Patents
Artificial propagation method for acrossocheilus yunnanensis Download PDFInfo
- Publication number
- CN103444610A CN103444610A CN2013104339903A CN201310433990A CN103444610A CN 103444610 A CN103444610 A CN 103444610A CN 2013104339903 A CN2013104339903 A CN 2013104339903A CN 201310433990 A CN201310433990 A CN 201310433990A CN 103444610 A CN103444610 A CN 103444610A
- Authority
- CN
- China
- Prior art keywords
- water
- artificial
- hatching
- propagation
- body weight
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Landscapes
- Farming Of Fish And Shellfish (AREA)
Abstract
The invention relates to an artificial propagation method for acrossocheilus yunnanensis, and belongs to the technical field of fish farming. The artificial propagation method includes the steps of intensive parent fish cultivation, artificial parturition hastening, artificial insemination and artificial incubation. The artificial propagation method has the advantages that mature propagation parents can be cultivated stably under environmental conditions with low water temperatures, and the method is applicable to artificial propagation of the acrossocheilus yunnanensis in highland cold water environments; artificial control degree is high in the process of propagation of the acrossocheilus yunnanensis, entire artificial medicine parturition hastening, artificial insemination and artificial incubation are realized, propagation process is stable and controllable, and the method is applicable to large-scale fry breeding according to needs; the method is simple, efficient and beneficial to popularization and application.
Description
Technical field
The present invention relates to a kind of artificial fecundation method of Acrossocheilus yunnanensis, belong to the fish culture technical field.
Background technology
Acrossocheilus yunnanensis (Acrossocheilus yunnanensis) belongs to Cypriniformes, Cyprinidae, and Barbinae, light lip fish belongs to.The middle-size and small-size fish with the important ecological value and certain economic worth.Be distributed in Pearl River system, ChangJiang River upstream and tributary ，Wei China Endemic fish thereof, type locality, in Yunnan, has the plateau of adaptation cold water environment, and individuality is the characteristics of large (common individuality reaches 20～30cm).Due to reasons such as hydraulic engineering construction and river pollution, overfishings, Acrossocheilus yunnanensis germ plasm resource amount sharply reduces in recent years, and trace has been difficult to find on many ground, Yunnan, and the Yunnan Central Yunnan only has a small amount of natural population in the indivedual cold water dragon's pools of Dian Chi periphery.Recover Acrossocheilus yunnanensis germ plasm resource and become the important research contents of Yunnan Province's fishery.Make good use of this Species of Rare Fish from Qingdao resource in order to protect, from 2010, unit of the present invention adhered to collecting the Acrossocheilus yunnanensis wild germplasm, and carried out aspect researchs such as raising and train cultivation, artificial propagation and seed rearing.
The present invention is intended to carry out artificial propagation techniques research and the application of the Acrossocheilus yunnanensis cultivated under the cultivating condition of altitude environment (Yunnan Central Yunnan) cold water pond.Through document retrieval, do not find at home the report identical with the present invention.
Summary of the invention
The object of the present invention is to provide a kind of artificial fecundation method of Acrossocheilus yunnanensis, that is: by the Acrossocheilus yunnanensis of domesticating and cultivating under the cultivating condition of cold water pond, by parent fish rearing, reach sexual maturity, implement artificial induced spawning, hatching, the successful reproduction filial generation.
The artificial fecundation method of a kind of Acrossocheilus yunnanensis of the present invention, its concrete steps are as follows:
A. parent population reinforced cultivating
The maturation individuality that selection total length in 3～5 age reaches 20～30cm carries out special pond reinforced cultivating as parent population, cultivates pool area 100～300m
2, the depth of water 0.8～1.2m, more than dissolved oxygen 5mg/L, water body on the 3rd～4 exchange once, keeps that water body is limpid pool wall to give birth to appropriate thread algae to ingest for parent population; The carp perfect compound feed that particle diameter 2mm albumen quality percentage is 37% of throwing something and feeding first 4 months of mating season, a day feeding volume is 3%～4% of fish body weight; The daily iron supplement long yellow mealworm young of 2～3cm of throwing something and feeding, feeding volume is fish body weight 0.3%;
B. artificial induced spawning
, carry out parent population and hasten parturition during 14.5 ℃～18 ℃ of water temperatures in mid or late April; Pectoral fin base portion injection oxytocic drug: maleic acid DOM (DOM) 5～7mg/kg fish body weight+luteinizing hormone releasing hormone A
2(LHRH-A
2) 6～8ug/kg fish body weight+human chorionic gonadtropin (HCG), 1000～1300 units/kg fish body weight, the milter injected dose is raun 1/3～1/2; After the oxytocic drug injection, parent population is placed in storage pond, holding pond shading foster and stimulation by running water temporarily, and water temperature is controlled at 15 ± 1 ℃, and the effect time is 35～38h;
C. artificial insemination
The dry method of artificial fertilization: fish-egg and milter seminal fluid are 1:1～2 according to the male and female ratio, clamp-on in the ceramic whiteware basin of drying, with chicken feather, fully stir 1～2 minute, adding a little clear water stirs 1～2 minute again, make it abundant fertilization, the standing 40min of plastic big basin container that fertilized egg being placed in be stirred is equipped with to clear water cleans fertilized egg 1～2 time with clear water again after the abundant imbibition egg membrane of fertilized egg lifts 30～40min, waits for hatching;
D. artificial incubation
Plastic big basin Lentic hatching and two kinds of modes of floating hatching frame miniflow water hatching are taked in artificial incubation; The plastic big basin 20L that is filled with water, place 3000～4000 pieces, fertilized egg, and indoor temperature is controlled at 15～16 ℃, and shading, standing hatching are changed water every day 2 times sooner or later, change water 1/2 at every turn; Floating incubation plate is to cut down and merge with the batten saw of the wide 6cm of thick 1cm the rectangle wooden frame that nail is made 50cm * 100cm, and wooden frame bottom surface nail presses the open and flat tight bolting silk sheet of 40 order to make; Incubation plate floats on hatchery, 8000～10000 pieces, every dish lay individual layer fertilized egg, hatchery area 5～10m
2, the depth of water 0.3～0.5m, the covered with sunshade net shading, keep miniflow water and water temperature constant, hatchery water day exchange 3～4 times; Two kinds of modes, when 15～16 ℃ of water temperatures, through 144～192h, are hatched prelarva, and average incubation rate reaches 78.3%.
The invention has the advantages that:
1, just can stably cultivate ripe breeding parent under low water temperature environmental condition, be suitable for the artificial propagation that the plateau cold water environment is carried out Acrossocheilus yunnanensis.
2, Acrossocheilus yunnanensis reproductive process Artificial Control degree is high, and for total man's work medicine is hastened parturition, artificial insemination and hatching, process stabilization is controlled, suitablely by demand, carries out the scale seed breeding;
3, have advantages of that method is easy, efficient, be beneficial to and apply.
Embodiment
In the invention process, parent population reinforced cultivating feed, artificial induced spawning medication are the market purchase.
The inventor adopts the inventive method to carry out the research of Acrossocheilus yunnanensis artificial propagation techniques in aquatic products technical advice station, Yunnan Province subordinate's cold water fish culture experiment base year July in January, 2012 to 2013, the breeding parent is the cultivation screening through 2～3 years by the wild Acrossocheilus yunnanensis fingerling of unit of the present invention domesticating and cultivating.In mid or late April, 2013 is chosen 20 groups of mature brood fish, carries out the artificial induced spawning injection, and spawning rate reaches 90%, realizes artificial propagation Acrossocheilus yunnanensis 18.7 ten thousand tails.Incubation rate reaches 78.3%, and extremely flat trip stage survival rate of fish fry reaches 81.2%.Embodiment is as follows:
A. parent population reinforced cultivating
Before mating season 4 months, select to reach individual large, the Acrossocheilus yunnanensis that physique is healthy and strong of age at sexual maturity (3～5 age) as parent population, general total length reaches 20～30cm.Carry out special pond reinforced cultivating, the reinforced cultivating pond adopts cement flowing water pond, area 300m
2, depth of water 1.2m, more than dissolved oxygen 5mg/L, water body exchange on the 3rd～4 is once.The occurring in nature Acrossocheilus yunnanensis mainly be take thread algae as food, therefore keep water body limpid so that at the bottom of pond pool wall and give birth to appropriate thread algae and ingest for the Acrossocheilus yunnanensis parent population.The carp perfect compound feed that the particle diameter 2mm albumen quality of throwing something and feeding percentage is 37%, a day feeding volume is 3～4% of fish body weight; The daily iron supplement long yellow mealworm young of 2～3cm of throwing something and feeding, feeding volume is 0.3% of fish body weight;
B. artificial induced spawning
About mid-April, in the time of between 14.5 ℃～18 ℃ of water temperatures, select the parent population that physique is healthy and strong, gonad development is good to carry out artificial induced spawning.General ripe raun belly is larger, softness and high resilience, and side sexual gland profile is obvious, gently presses belly, can see long ± 0.5～1.0cm of gonapophysis, white powder.The milter health is more slender, a rhynchodaenm and side scale adularescent pearl star, and the full soft degree of belly is not as good as raun, and when ripe, the light belly of pressing can flow out dense thick white seminal fluid from gonopore.The injection of hastening parturition adopts the oxytocins mixed solvent.The combination of raun oxytocic drug and dosage are: maleic acid DOM (DOM) 5～7mg/kg fish body weight+luteinizing hormone releasing hormone A
2(LHRH-A
2) 6～8ug/kg fish body weight+human chorionic gonadtropin (HCG), 1000～1300 units/kg fish body weight.Injection site is the pectoral fin base portion.Male parent population injectable drug and the same raun of method, dosage is raun 1/3～1/2.Parent population after the oxytocic drug injection is put into to the small-sized cement storage pond, holding pond, and carry out stimulation by running water.Excited because of meeting performance after the milter drug injection, happiness is jumped, and parent population storage pond, holding pond need suitable for reading are added a cover sunshade net and prevented escaping and being used for shading.When water temperature is controlled at 15 ± 1 ℃, the Acrossocheilus yunnanensis medicine ecbolic effect time is 35～38h.
C. artificial insemination
The dry method of artificial fertilization: fish-egg and milter seminal fluid are 1:1～2 according to the male and female ratio, clamp-on in the ceramic whiteware basin of drying, with chicken feather, fully stir 1～2 minute, adding a little clear water stirs 1～2 minute again, make it abundant fertilization, the standing 40min of plastic big basin container that fertilized egg being placed in be stirred is equipped with to clear water cleans fertilized egg 1 to 2 time with clear water again after the abundant imbibition egg membrane of fertilized egg lifts 30～40min, waits for hatching.
D. artificial incubation
Example one:
Plastic big basin hatching pattern: plastic big basin (specification: 60cm suitable for reading * end opening 51cm * dark 21cm) every basin 20L that is filled with water, place 3000～4000 pieces, Acrossocheilus yunnanensis fertilized egg, indoor temperature is controlled at 15～16 ℃, shading, standing hatching, change water every day 2 times sooner or later, changes water 1/2 at every turn, when 15～16 ℃ of water temperatures, through 144～192h, hatch prelarva, incubation rate reaches 78.3%.This hatching pattern is applicable to hatching on a small scale.
Example two:
Floating incubation plate miniflow water hatching pattern: this is applicable to fairly large hatching, can in the hatching workshop, carry out.Floating incubation plate production method: use thickness 1cm, the batten saw of width 6cm is cut down and merge the rectangle wooden frame that nail is made 50cm * 100cm, and wooden frame bottom surface nail presses the open and flat tight bolting silk sheet of 40 order.Incubation plate row blow up floats on the water surface of hatchery, and every dish holds 8000～10000 pieces, fertilized egg, at the bottom of fertilized egg is laid in incubation plate, just is immersed in below the water surface.Hatchery area 6m
2, the depth of water 0.3～0.5m, the covered with sunshade net shading, keep miniflow water and water temperature constant, Spawning water day exchange 3～4 times.When 15～16 ℃ of water temperatures, through 144～192h, hatch the Acrossocheilus yunnanensis prelarva, incubation rate reaches 78.3%.
The prelarva that example one or example two are hatched is transferred in time the fry rearing pond of inner wall smooth and supports temporarily, and after absorbing complete flat trip to yolk sac, the cultivation of fry opening is carried out in the outdoor fish pond of transposition.
Claims (1)
1. the artificial fecundation method of an Acrossocheilus yunnanensis is characterized in that the concrete steps of artificial fecundation method of Acrossocheilus yunnanensis are as follows:
A. parent population reinforced cultivating
The maturation individuality that selection total length in 3～5 age reaches 20～30cm carries out special pond reinforced cultivating as parent population, cultivates pool area 100～300m
2, the depth of water 0.8～1.2m, more than dissolved oxygen 5mg/L, water body on the 3rd～4 exchange once, keeps that water body is limpid pool wall to give birth to appropriate thread algae to ingest for parent population; The carp perfect compound feed that particle diameter 2mm albumen quality percentage is 37% of throwing something and feeding first 4 months of mating season, a day feeding volume is 3%～4% of fish body weight; The daily iron supplement long yellow mealworm young of 2～3cm of throwing something and feeding, feeding volume is 0.3% of fish body weight;
B. artificial induced spawning
, carry out parent population and hasten parturition during 14.5 ℃～18 ℃ of water temperatures in mid or late April; Pectoral fin base portion injection oxytocic drug: maleic acid DOM (DOM) 5～7mg/kg fish body weight+luteinizing hormone releasing hormone A
2(LHRH-A
2) 6～8ug/kg fish body weight+human chorionic gonadtropin (HCG), 1000～1300 units/kg fish body weight, the milter injected dose is raun 1/3～1/2; After the oxytocic drug injection, parent population is placed in storage pond, holding pond shading foster and stimulation by running water temporarily, and water temperature is controlled at 15 ± 1 ℃, and the effect time is 35～38h;
C. artificial insemination
The dry method of artificial fertilization: fish-egg and milter seminal fluid are 1:1～2 according to the male and female ratio, clamp-on in the ceramic whiteware basin of drying, with chicken feather, fully stir 1～2 minute, adding a little clear water stirs 1～2 minute again, make it abundant fertilization, the standing 40min of plastic big basin container that fertilized egg being placed in be stirred is equipped with to clear water cleans fertilized egg 1～2 time with clear water again after the abundant imbibition egg membrane of fertilized egg lifts 30～40min, waits for hatching;
D. artificial incubation
Plastic big basin Lentic hatching and two kinds of modes of floating hatching frame miniflow water hatching are taked in artificial incubation; The plastic big basin 20L that is filled with water, place 3000～4000 pieces, fertilized egg, and indoor temperature is controlled at 15～16 ℃, and shading, standing hatching are changed water every day 2 times sooner or later, change water 1/2 at every turn; Floating incubation plate is to cut down and merge with the batten saw of the wide 6cm of thick 1cm the rectangle wooden frame that nail is made 50cm * 100cm, and wooden frame bottom surface nail presses the open and flat tight bolting silk sheet of 40 order to make; Incubation plate floats on hatchery, 8000～10000 pieces, every dish lay individual layer fertilized egg, hatchery area 5～10m
2, the depth of water 0.3～0.5m, the covered with sunshade net shading, keep miniflow water and water temperature constant, hatchery water day exchange 3～4 times; Two kinds of modes, when 15～16 ℃ of water temperatures, through 144～192h, are hatched prelarva.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310433990.3A CN103444610B (en) | 2013-09-23 | 2013-09-23 | Artificial propagation method for acrossocheilus yunnanensis |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310433990.3A CN103444610B (en) | 2013-09-23 | 2013-09-23 | Artificial propagation method for acrossocheilus yunnanensis |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103444610A true CN103444610A (en) | 2013-12-18 |
| CN103444610B CN103444610B (en) | 2014-09-17 |
Family
ID=49727795
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310433990.3A Expired - Fee Related CN103444610B (en) | 2013-09-23 | 2013-09-23 | Artificial propagation method for acrossocheilus yunnanensis |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103444610B (en) |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104509469A (en) * | 2014-12-24 | 2015-04-15 | 休宁县龙堂生态渔业科技有限公司 | High-density green ecological aquaculture method for acrossocheilus fasciatus |
| CN104855310A (en) * | 2015-05-06 | 2015-08-26 | 龙南源头活水生态科技有限责任公司 | Acrossocheilus fasciatus simulated ecological factory large scale breeding and domestication method |
| CN106577393A (en) * | 2016-12-16 | 2017-04-26 | 安徽省农业科学院水产研究所 | Artificial propagation method for breeding acrossocheilus fasciatus |
| CN107897052A (en) * | 2017-09-05 | 2018-04-13 | 龙泉市富溪农业发展有限公司 | A kind of grouper artificial breeding germplasm method for improving and its device |
| CN110741985A (en) * | 2019-11-19 | 2020-02-04 | 西双版纳云博水产养殖开发有限公司 | Artificial propagation method of sinocyclocheilus grahami |
| CN111248117A (en) * | 2020-01-16 | 2020-06-09 | 谢张群 | Breeding method of acrossocheilus fasciatus |
| CN111280092A (en) * | 2019-12-16 | 2020-06-16 | 贵州工程应用技术学院 | Artificial breeding method for acrossocheilus yunnanensis |
| CN117296756A (en) * | 2023-10-31 | 2023-12-29 | 珠江水资源保护科学研究所 | Artificial propagation method for Alcheilus longifolia |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102090361A (en) * | 2010-12-20 | 2011-06-15 | 新昌县沃洲鱼类开发研究所 | Artificial breeding method for Acrossocheilus fasciatus |
| CN102599096A (en) * | 2012-04-17 | 2012-07-25 | 中国科学院昆明动物研究所 | Method for carrying out incubation on neolissochilus benasi through artificial fertilization |
| CN103098737A (en) * | 2013-02-26 | 2013-05-15 | 浙江师范大学 | Manpower hasten parturition method of acrossocheilus fasciatus |
-
2013
- 2013-09-23 CN CN201310433990.3A patent/CN103444610B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102090361A (en) * | 2010-12-20 | 2011-06-15 | 新昌县沃洲鱼类开发研究所 | Artificial breeding method for Acrossocheilus fasciatus |
| CN102599096A (en) * | 2012-04-17 | 2012-07-25 | 中国科学院昆明动物研究所 | Method for carrying out incubation on neolissochilus benasi through artificial fertilization |
| CN103098737A (en) * | 2013-02-26 | 2013-05-15 | 浙江师范大学 | Manpower hasten parturition method of acrossocheilus fasciatus |
Non-Patent Citations (5)
| Title |
|---|
| 刘丽丽等: "半刺厚唇鱼的人工繁殖试验", 《淡水渔业》, vol. 40, no. 2, 31 March 2010 (2010-03-31) * |
| 巫一安等: "光唇鱼的驯养与人工繁殖试验", 《水产养殖》, no. 11, 30 November 2009 (2009-11-30) * |
| 张玉明等: "光唇鱼人工繁殖研究", 《浙江海洋学院学报(自然科学版)》, vol. 29, no. 3, 31 May 2010 (2010-05-31) * |
| 李维贤: "云南光唇鱼的生态观察", 《动物学杂志》, no. 2, 29 February 1976 (1976-02-29) * |
| 陈礼强等: "云南光唇鱼的人工繁殖研究", 《淡水渔业》, vol. 36, no. 1, 31 January 2006 (2006-01-31) * |
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104509469A (en) * | 2014-12-24 | 2015-04-15 | 休宁县龙堂生态渔业科技有限公司 | High-density green ecological aquaculture method for acrossocheilus fasciatus |
| CN104855310A (en) * | 2015-05-06 | 2015-08-26 | 龙南源头活水生态科技有限责任公司 | Acrossocheilus fasciatus simulated ecological factory large scale breeding and domestication method |
| CN106577393A (en) * | 2016-12-16 | 2017-04-26 | 安徽省农业科学院水产研究所 | Artificial propagation method for breeding acrossocheilus fasciatus |
| CN107897052A (en) * | 2017-09-05 | 2018-04-13 | 龙泉市富溪农业发展有限公司 | A kind of grouper artificial breeding germplasm method for improving and its device |
| CN110741985A (en) * | 2019-11-19 | 2020-02-04 | 西双版纳云博水产养殖开发有限公司 | Artificial propagation method of sinocyclocheilus grahami |
| CN111280092A (en) * | 2019-12-16 | 2020-06-16 | 贵州工程应用技术学院 | Artificial breeding method for acrossocheilus yunnanensis |
| CN111280092B (en) * | 2019-12-16 | 2022-03-08 | 贵州工程应用技术学院 | Artificial breeding method for acrossocheilus yunnanensis |
| CN111248117A (en) * | 2020-01-16 | 2020-06-09 | 谢张群 | Breeding method of acrossocheilus fasciatus |
| CN117296756A (en) * | 2023-10-31 | 2023-12-29 | 珠江水资源保护科学研究所 | Artificial propagation method for Alcheilus longifolia |
| CN117296756B (en) * | 2023-10-31 | 2024-05-28 | 珠江水资源保护科学研究所 | Artificial propagation method for Alcheilus longifolia |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103444610B (en) | 2014-09-17 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103444610B (en) | Artificial propagation method for acrossocheilus yunnanensis | |
| CN103563799B (en) | Low temperature breeds the method for Crucian Carp Fry | |
| KR101281396B1 (en) | Spawning induction by change of environment for takifugu | |
| CN100431408C (en) | Method for breeding two hybrid species of fish | |
| CN103461248B (en) | Fish fry cultivation method of acrossocheilus yunnanensis | |
| CN101940184B (en) | Breeding and culturing method of improved crucian carp | |
| CN103931525B (en) | The artificial propagation of the naked skin-carp meat in Xinjiang and fry rearing method | |
| CN101103706B (en) | Reproduction method for American reeves shad artificial cultivation parent fish | |
| CN103960184B (en) | A kind of loach industrial artificial breeding improves the seedling-cultivating method of survival rate | |
| CN101960996A (en) | Artificial breeding method of Scaphesthes macrolepis fry | |
| CN104642212A (en) | Artificial breeding method for onychostoma simus | |
| CN103461253B (en) | Yellow River fish artificial fecundation method | |
| CN104170778A (en) | Large-scale common sucker breeding method capable of controlling temperature of circulating water | |
| CN108142328B (en) | Early propagation and rapid seedling raising method for small yellow croakers | |
| CN101647412B (en) | Method for breeding tridentiger trigonocephalus by artificial induced spawning and insemination | |
| CN114651751B (en) | Artificial breeding method for yellow lip fish | |
| CN104255615B (en) | It is a kind of that soft thorn is naked splits buttocks fish artificial fecundation method | |
| CN102919186B (en) | Artificial breeding method for sillago sihama | |
| CN107087560B (en) | Establishment and application of ecological polyculture mode of penaeus vannamei boone and golden pompano | |
| CN104642211A (en) | Artificial breeding method for procypris rabaudi | |
| CN103081828B (en) | Method for artificially propagating parabramis pekinensis | |
| CN108522365A (en) | A kind of method of Australian silverbelly large-scale artificial nursery | |
| CN107926770A (en) | Epinephelus akaara and epinephelus lanceolatus fish distant hybrid breeding method | |
| CN113331087A (en) | Artificial propagation method of Gymnocypris ventricosa | |
| CN110178760B (en) | Method for breeding high-gynogenetic fry of parent fish of cynoglossus semilaevis in nutrition regulation and control manner |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20140917 Termination date: 20150923 |
|
| EXPY | Termination of patent right or utility model |