CN110741985A - Artificial propagation method of sinocyclocheilus grahami - Google Patents

Artificial propagation method of sinocyclocheilus grahami Download PDF

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Publication number
CN110741985A
CN110741985A CN201911132557.XA CN201911132557A CN110741985A CN 110741985 A CN110741985 A CN 110741985A CN 201911132557 A CN201911132557 A CN 201911132557A CN 110741985 A CN110741985 A CN 110741985A
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fish
female
male
parent
artificial propagation
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王云清
王敏奇
李燕
李怡凌
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Xishuangbanna Yunbo Aquaculture Development Co Ltd
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Xishuangbanna Yunbo Aquaculture Development Co Ltd
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Priority to CN201911132557.XA priority Critical patent/CN110741985A/en
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K61/00Culture of aquatic animals
    • A01K61/10Culture of aquatic animals of fish
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K61/00Culture of aquatic animals
    • A01K61/10Culture of aquatic animals of fish
    • A01K61/17Hatching, e.g. incubators
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Marine Sciences & Fisheries (AREA)
  • Zoology (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Farming Of Fish And Shellfish (AREA)

Abstract

The invention provides an artificial propagation method of sinocyclocheilus grahami, which comprises the steps of parent fish selection, parent fish cultivation and artificial propagation. The invention adopts a scientific artificial propagation method to cultivate the sinocyclocheilus grahami, has high fertilization and hatching rate, solves the problem that the wild sinocyclocheilus grahami is excessively caught and has an acute reduction in quantity for economic purposes, and explores and realizes the artificial propagation of the sinocyclocheilus grahami.

Description

Artificial propagation method of sinocyclocheilus grahami
Technical Field
The invention belongs to the technical field of artificial propagation of fishes, and particularly relates to an artificial propagation method of sinocyclocheilus grahami.
Background
Grahami, name: barbodes huangchucheni (Tchang, 1962), with flat body and curved back and abdomen contours. The head length is less than the body height. Slightly shorter, sharp. Inferior oral position. Thin and smooth lip. 2 pairs of them are needed, long and well-developed. The side line scales are 33-39. The dorsal fin is thorny and is usually smaller than the head length, and the rear edge is provided with sawteeth.
Back fin lines 4, 8; hip fins 3, 5; 1, 15-16 parts of chest fin rays; ventral fin rays 1, 8. Side line scale 31[ (6-7)/(3-V) 36, peristrophe scale 14-16; anterior dorsal fin scales 12-14. Hypopharynx teeth 3 rows, 2.3.5. 8-11 parts of gill rake.
The standard length is 2.7-3.8 times of the height of the body (x + -s =3.3 + -0.38), 4.0-4.6 times of the head length (4.2 + -0.13), 4.9-6.6 times of the caudal peduncle length (5.6 + -0.53), and 8.1-9.4 times of the caudal peduncle height (8.5 + -0.31). The length of the head is 3.1-3.8 (3.3 +/-0.23) times of the length of the kiss, 3.3-4.7 (3.8 +/-0.37) times of the diameter of the eyes and 2.4-3.3 (3.0 +/-0.29) times of the distance between the eyes.
The body is flat, and the contour lines of the back and the abdomen are slightly arc-shaped. The head is flat and the length is smaller than the height of the body. The length of the tip of the kiss point is shorter than that of the back head of the eye, the kiss skin is stopped at the base of the upper lip, and the anterior edge of the anterior orbital bone is provided with a sulcus oblique angle. The lower part of the mouth is in the shape of a horseshoe, and the upper jaw extends backwards to the vertical line of the nostril or the anterior border of the eye. The lips are thin and simple, with the upper and lower lips joined at the corners of the mouth. The left and right posterior labial sulcus extend forward and are not connected at the Yizhen part, and the distance between them is 1/3-1/2 of the eye diameter. 2 pairs of developed palpitations, the palpitations of the kiss are thinner than the palpitations of the jaw, and the posterior extension can reach the anterior half of the eye or exceed the anterior half of the eye; the jawbone whisker extends posteriorly beyond the posterior edge of the eye. The upper part of the eye is wide and raised, and the nostril is closer to the anterior border than the rhyncline. The branchial membrane is attached to the branchial isthmus below the posterior margin of the anterior branchial canopy, with a spacing of about 1/2 inches of eye diameter. The gill rake is short and sparsely arranged. The hypopharynx has flat side, sharp and curved top, the first tooth of the ventral surface of the main part is the smallest, and the second tooth is the largest.
The outer edge of the dorsal fin is concave, the tail root of the dorsal fin is not branched, the fin is thick, the rear edge of the dorsal fin is provided with sawteeth, the length of the sawteeth is shorter than or equal to that of the head, and the starting point of the sawteeth is equal to the distance from the tail fin base to eyes or nose. The pectoral fin is slightly shorter than the head length, does not reach the starting point of the ventral fin by backward extension, has a distance of about 2-3 scales, is shorter than the pectoral fin, and has the starting point before the starting point of the dorsal fin, the basal end point of the ventral fin and the starting point of the dorsal fin are on the same vertical line, and the dorsal fin does not reach the anus by backward bending. The starting point of the hip fin is equal to or closer to the starting point of the ventral fin and the base of the tail fin, and the hip fin does not extend backwards to the base of the tail fin. The tail fins are bifurcated with the longest fin ray being about 2 times as strong as the shortest fin ray. The anus is immediately forward of the starting point of the anal fin.
The scales are medium and large, the scales of the chest are small, the axillary scales are arranged at the base of the ventral fin, and the scaly sheaths are arranged at the base of the dorsal and the gluteal fins. The lateral line is complete and slightly arc-shaped, is straight backwards and extends to the middle point of the tail fin base. The swimming bladder has 2 chambers, the front chamber is short, and the rear chamber is long and is about 2 times of the front chamber. Dark brown peritoneum. The intestine is about 3 times as long as the standard.
The back side of the body is dark, the ventral side is shallow, the center of the tail fin base is provided with a black spot, and the upper and lower fin lines on the outermost side of the tail fin are blackest. Mainly distributed in water systems such as the cang river, the red river and the like.
Disclosure of Invention
The invention provides an artificial propagation method of sinocyclocheilus grahami, which has the specific technical scheme that:
an artificial propagation method of sinocyclocheilus grahami comprises the following steps:
firstly, parent fish source and seed selection: the parent is obtained by wild capture from the billows and domestication and selection;
secondly, parent fish cultivation:
(1) a cultivation pool:
the parent fish culture pond has sufficient water source, fresh water quality, convenient injection and drainage and quiet environment; the pool bottom is flat, the sludge is less, the area is 0.2h, the square meter is 0.33h, and the water depth is 1.5m to 2 m; the pond clearing is in accordance with SC/T1008-2002.
(2) And (3) male and female matching:
the male and female proportion is as follows: 1: 1;
(3) stocking amount:
the stocking amount of the parent fish is 100kg/100 square meters or the stocking density is 300-800 per 100 square meters;
(4) feeding and feeding:
the sinocyclocheilus grahami belongs to omnivorous fishes, and the parent fish feed is mainly a palatable live bait; the daily bait feed accounts for 2% -3% of the weight of the fish, and is supplemented with compound feed, and the fish bait feed mainly comprises the following components: 30% of protein, 3.5% of fat, 11% of crude fiber, 15% of crude ash powder and 1.3% of lysine, and the feeding amount is adjusted according to seasons and water temperature;
thirdly, artificial propagation:
(1) parent fish selection and matching:
the selection criteria were: the female fish is more than 3+ old, the weight is more than 0.5kg, and the abdomen is enlarged and soft; the male fish is more than 2+ years old, the weight is more than 0.3 kg, and semen flows out when the male fish lightly presses the abdomen; the male and female proportion is as follows: 1: 1;
(2) injection amount and method of oxytocin:
LRH-A2+ DOM is injected into the male and female fish at one time, female fish with the injection amount is LRH-A22 mug-5 mug, DOM is 2 mg-5 mg, and the fish is half of the female fish, and the male and female fish are synchronously injected at one time; the test result shows that the injection amount of the oxytocic is LRH-A23 mug + DOM 3mg, the fertilization rate reaches more than 90 percent, and the oxytocic is most suitable.
(3) Artificial fertilization:
when the spawned parent fish is going to spawn in the high-tide of estrus, spawn collection and semen collection are carried out, so that mature semen and spawn are fertilized in a container;
(4) hatching:
and (4) debonding the fertilized eggs, transferring the fertilized eggs into an incubation pool for running water incubation or scattering the fertilized eggs on the palm sheets for still water incubation.
Further, in the step two (3), the filter-feeding fishes are put in the parent fish putting-in pond in a matching way.
Further, in the second step (4), the palatable live baits are live fishes, live shrimps and live insects.
Further, the time for artificial propagation is 1-4 months per year.
Further, in the third step (4), the hatching in flowing water is performed by adopting an annular flowing water groove.
Further, in the third step (4), the incubation temperature is 18-24 ℃.
The invention has the beneficial effects that: the method has the advantages that the method is scientific in artificial propagation method for cultivating the sinocyclocheilus grahami, fertilization and hatching rate are high, the problem that the number of the sinocyclocheilus grahami is sharply reduced due to excessive fishing of the sinocyclocheilus grahami in the economic purpose is solved, and the artificial propagation of the sinocyclocheilus grahami is explored and realized.
Detailed Description
Example 1:
an artificial propagation method of sinocyclocheilus grahami comprises the following steps:
firstly, parent fish source and seed selection: the parent is obtained by wild capture from the billows and domestication and selection;
secondly, parent fish cultivation:
(1) a cultivation pool:
the parent fish culture pond has sufficient water source, fresh water quality, convenient injection and drainage and quiet environment; the pool bottom is flat, the sludge is less, the area is 0.2h, and the water depth is 1.5 m;
(2) and (3) male and female matching:
the male and female proportion is as follows: 1: 1;
(3) stocking amount:
the stocking amount of the parent fish is 100kg/100 square meters or the stocking density is 300 pieces/100 square meters;
(4) feeding and feeding:
the sinocyclocheilus grahami belongs to omnivorous fishes, and the parent fish feed is mainly a palatable live bait; the daily bait feed is 2 percent of the weight of the fish body, is supplemented with compound feed, and comprises the following main components: 30% of protein, 3.5% of fat, 11% of crude fiber, 15% of crude ash powder and 1.3% of lysine, and the feeding amount is adjusted according to seasons and water temperature;
thirdly, artificial propagation:
(1) parent fish selection and matching:
the selection criteria were: the female fish is more than 3+ old, the weight is more than 0.5kg, and the abdomen is enlarged and soft; the male fish is more than 2+ years old, the weight is more than 0.3 kg, and semen flows out when the male fish lightly presses the abdomen; the male and female proportion is as follows: 1: 1;
(2) injection amount and method of oxytocin:
LRH-A2+ DOM is injected into the male and female fish at one time, the injected female fish is LRH-A22 mug, DOM 2mg, and the fish is half of the female fish, and the injection is synchronously performed at one time;
(3) artificial fertilization:
when the spawned parent fish is going to spawn in the high-tide of estrus, spawn collection and semen collection are carried out, so that mature semen and spawn are fertilized in a container;
(4) hatching:
and (4) debonding the fertilized eggs, transferring the fertilized eggs into an incubation pool for running water incubation or scattering the fertilized eggs on the palm sheets for still water incubation.
Further, in the step two (3), the filter-feeding fishes are put in the parent fish putting-in pond in a matching way.
Further, in the second step (4), the palatable live baits are live fishes, live shrimps and live insects.
Further, the time for artificial propagation is 1-4 months per year.
Further, in the third step (4), the hatching in flowing water is performed by adopting an annular flowing water groove.
Further, in the third step (4), the incubation temperature is 18-24 ℃.
The hatchability of the water at 20-22 ℃ is best, and can reach more than 80%.
Example 2:
an artificial propagation method of sinocyclocheilus grahami comprises the following steps:
firstly, parent fish source and seed selection: the parent is obtained by wild capture from the billows and domestication and selection;
secondly, parent fish cultivation:
(1) a cultivation pool:
the parent fish culture pond has sufficient water source, fresh water quality, convenient injection and drainage and quiet environment; the pool bottom is flat, the sludge is less, the area is 0.25 square meter, and the water depth is 1.75 m;
(2) and (3) male and female matching:
the male and female proportion is as follows: 1: 1;
(3) stocking amount:
the stocking amount of parent fishes is 100kg/100 square meters or the stocking density is 500 pieces/100 square meters;
(4) feeding and feeding:
the sinocyclocheilus grahami belongs to omnivorous fishes, and the parent fish feed is mainly a palatable live bait; the daily bait feed accounts for 2% -3% of the weight of the fish, and is supplemented with compound feed, and the fish bait feed mainly comprises the following components: 30% of protein, 3.5% of fat, 11% of crude fiber, 15% of crude ash powder and 1.3% of lysine, and the feeding amount is adjusted according to seasons and water temperature;
thirdly, artificial propagation:
(1) parent fish selection and matching:
the selection criteria were: the female fish is more than 3+ old, the weight is more than 0.5kg, and the abdomen is enlarged and soft; the male fish is more than 2+ years old, the weight is more than 0.3 kg, and semen flows out when the male fish lightly presses the abdomen; the male and female proportion is as follows: 1: 1;
(2) injection amount and method of oxytocin:
LRH-A2+ DOM is injected into the male and female fish at one time, the female fish with the injection amount is LRH-A23 mu g and DOM 3mg, and the fish is half of the female fish in the amount, and the injection is synchronously performed at one time; the test result shows that the injection amount of the oxytocic is LRH-A23 mug + DOM 3mg, the fertilization rate reaches more than 90 percent, and the oxytocic is most suitable.
(3) Artificial fertilization:
when the spawned parent fish is going to spawn in the high-tide of estrus, spawn collection and semen collection are carried out, so that mature semen and spawn are fertilized in a container;
(4) hatching:
and (4) debonding the fertilized eggs, transferring the fertilized eggs into an incubation pool for running water incubation or scattering the fertilized eggs on the palm sheets for still water incubation.
Further, in the step two (3), the filter-feeding fishes are put in the parent fish putting-in pond in a matching way.
Further, in the second step (4), the palatable live baits are live fishes, live shrimps and live insects.
Further, the time for artificial propagation is 1-4 months per year.
Further, in the third step (4), the hatching in flowing water is performed by adopting an annular flowing water groove.
Further, in the third step (4), the incubation temperature is 18-24 ℃.
The hatchability of the water at 20-22 ℃ is best, and can reach more than 80%.
Example 3:
an artificial propagation method of sinocyclocheilus grahami comprises the following steps:
firstly, parent fish source and seed selection: the parent is obtained by wild capture from the billows and domestication and selection;
secondly, parent fish cultivation:
(1) a cultivation pool:
the parent fish culture pond has sufficient water source, fresh water quality, convenient injection and drainage and quiet environment; the pool bottom is flat, the sludge is less, the area is 0.33h, and the water depth is 2 m;
(2) and (3) male and female matching:
the male and female proportion is as follows: 1: 1;
(3) stocking amount:
the stocking amount of parent fishes is 100kg/100 square meters or the stocking density is 800 pieces/100 square meters;
(4) feeding and feeding:
the sinocyclocheilus grahami belongs to omnivorous fishes, and the parent fish feed is mainly a palatable live bait; the daily bait feed accounts for 2% -3% of the weight of the fish, and is supplemented with compound feed, and the fish bait feed mainly comprises the following components: 30% of protein, 3.5% of fat, 11% of crude fiber, 15% of crude ash powder and 1.3% of lysine, and the feeding amount is adjusted according to seasons and water temperature;
thirdly, artificial propagation:
(1) parent fish selection and matching:
the selection criteria were: the female fish is more than 3+ old, the weight is more than 0.5kg, and the abdomen is enlarged and soft; the male fish is more than 2+ years old, the weight is more than 0.3 kg, and semen flows out when the male fish lightly presses the abdomen; the male and female proportion is as follows: 1: 1;
(2) injection amount and method of oxytocin:
LRH-A2+ DOM is injected into the male and female fish at one time, the female fish with the injection amount is LRH-A25 mu g and DOM 5mg, and the fish is half of the female fish in the amount, and the injection is synchronously performed at one time;
(3) artificial fertilization:
when the spawned parent fish is going to spawn in the high-tide of estrus, spawn collection and semen collection are carried out, so that mature semen and spawn are fertilized in a container;
(4) hatching:
and (4) debonding the fertilized eggs, transferring the fertilized eggs into an incubation pool for running water incubation or scattering the fertilized eggs on the palm sheets for still water incubation.
Further, in the step two (3), the filter-feeding fishes are put in the parent fish putting-in pond in a matching way.
Further, in the second step (4), the palatable live baits are live fishes, live shrimps and live insects.
Further, the time for artificial propagation is 1-4 months per year.
Further, in the third step (4), the hatching in flowing water is performed by adopting an annular flowing water groove.
Further, in the third step (4), the incubation temperature is 18-24 ℃.
The hatchability of the water at 20-22 ℃ is best, and can reach more than 80%.

Claims (6)

1. An artificial propagation method of sinocyclocheilus grahami is characterized by comprising the following steps: firstly, parent fish source and seed selection: the parent is obtained by wild capture from the billows and domestication and selection; secondly, parent fish cultivation: (1) a culture pond: the parent fish culture pond has sufficient water source, fresh water quality, convenient injection and drainage and quiet environment; the pool bottom is flat, the sludge is less, the area is 0.2h, the square meter is 0.33h, and the water depth is 1.5m to 2 m; (2) male and female ratio: the male and female proportion is as follows: 1: 1; (3) stocking amount: the stocking amount of the parent fish is 100kg/100 square meters or the stocking density is 300-800 per 100 square meters; (4) feeding and feeding: the sinocyclocheilus grahami belongs to omnivorous fishes, and the parent fish feed is mainly a palatable live bait; the daily bait feed accounts for 2% -3% of the weight of the fish, and is supplemented with compound feed, and the fish bait feed mainly comprises the following components: 30% of protein, 3.5% of fat, 11% of crude fiber, 15% of crude ash powder and 1.3% of lysine, and the feeding amount is adjusted according to seasons and water temperature; thirdly, artificial propagation: (1) parent fish selection and matching: the selection criteria were: the female fish is more than 3+ old, the weight is more than 0.5kg, and the abdomen is enlarged and soft; the male fish is more than 2+ years old, the weight is more than 0.3 kg, and semen flows out when the male fish lightly presses the abdomen; the male and female proportion is as follows: 1: 1; (2) injection amount and method of oxytocin: LRH-A2+ DOM is injected into the male and female fish at one time, female fish with the injection amount is LRH-A22 mug-5 mug, DOM is 2 mg-5 mg, and the fish is half of the female fish, and the male and female fish are synchronously injected at one time; (3) artificial fertilization: when the spawned parent fish is going to spawn in the high-tide of estrus, spawn collection and semen collection are carried out, so that mature semen and spawn are fertilized in a container; (4) hatching: and (4) debonding the fertilized eggs, transferring the fertilized eggs into an incubation pool for running water incubation or scattering the fertilized eggs on the palm sheets for still water incubation.
2. The artificial propagation method of Sinocyclocheilus grahami according to claim 1, wherein in the second step (3), filter-feeding fishes are bred in the parent fish breeding pond.
3. The method for artificially propagating Sinocyclocheilus grahami according to claim 1, wherein in the second step (4), the palatable live baits are live fish, live shrimp and live insects.
4. The method for artificially propagating Sinocyclocheilus grahami according to claim 1, wherein the period of the artificial propagation is 1-4 months per year.
5. The artificial propagation method of sinocyclocheilus grahami according to claim 1, wherein in the third step (4), the incubation in flowing water is performed by using an annular flowing water tank.
6. The method for artificially propagating Sinocyclocheilus grahami according to claim 1, wherein in the third step (4), the incubation temperature is 18-24 ℃.
CN201911132557.XA 2019-11-19 2019-11-19 Artificial propagation method of sinocyclocheilus grahami Pending CN110741985A (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112741029A (en) * 2021-02-04 2021-05-04 中国科学院昆明动物研究所 Method for hybridizing sinocyclocheilus grahami and crucian carp
CN117223646A (en) * 2023-09-19 2023-12-15 西双版纳云博水产养殖开发有限公司 Crossbreeding method of sinocyclocheilus grahami and carp

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CN103444610A (en) * 2013-09-23 2013-12-18 云南省水产技术推广站 Artificial propagation method for acrossocheilus yunnanensis
CN110122380A (en) * 2019-06-04 2019-08-16 利津县双瀛水产苗种有限责任公司 A kind of north waters calico bass artificial breeding method
CN110301381A (en) * 2019-08-20 2019-10-08 西双版纳云博水产养殖开发有限公司 A kind of artificial fecundation method of diplopore fish
CN110384057A (en) * 2019-08-20 2019-10-29 西双版纳云博水产养殖开发有限公司 A kind of artificial fecundation method of four palpus Barb of big squama

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20100035327A1 (en) * 2008-08-11 2010-02-11 Ann Marie Steele Use of rice-derived products in a universal cell culture medium
CN103444610A (en) * 2013-09-23 2013-12-18 云南省水产技术推广站 Artificial propagation method for acrossocheilus yunnanensis
CN110122380A (en) * 2019-06-04 2019-08-16 利津县双瀛水产苗种有限责任公司 A kind of north waters calico bass artificial breeding method
CN110301381A (en) * 2019-08-20 2019-10-08 西双版纳云博水产养殖开发有限公司 A kind of artificial fecundation method of diplopore fish
CN110384057A (en) * 2019-08-20 2019-10-29 西双版纳云博水产养殖开发有限公司 A kind of artificial fecundation method of four palpus Barb of big squama

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112741029A (en) * 2021-02-04 2021-05-04 中国科学院昆明动物研究所 Method for hybridizing sinocyclocheilus grahami and crucian carp
CN117223646A (en) * 2023-09-19 2023-12-15 西双版纳云博水产养殖开发有限公司 Crossbreeding method of sinocyclocheilus grahami and carp
CN117223646B (en) * 2023-09-19 2024-03-01 西双版纳云博水产养殖开发有限公司 Crossbreeding method of sinocyclocheilus grahami and carp

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Application publication date: 20200204