CN117296756B - Artificial propagation method for Alcheilus longifolia - Google Patents
Artificial propagation method for Alcheilus longifolia Download PDFInfo
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The application provides an artificial propagation method of longfin cheilippine, which comprises the following steps: selecting wild longfin cheilinus cinereus of proper age and weight of 350-500 g in advance for gradient domestication and feeding; placing the longfin cheilinus comatus into an indoor circulating water culture pond for culture to obtain the fish to be subjected to reinforced induced spawning; carrying out intensified cultivation and cultivation on the obtained spawning-accelerating spawning-waiting fish to promote gonad development of the spawning fish; screening the breeding fish subjected to the intensified cultivation, and then carrying out anesthesia treatment on the screened breeding fish; injecting an oxytocic into the anesthetized breeding fish; placing parent fish injected with the oxytocic into an oxytocic pool for artificial oxytocic fertilization; hatching fertilized eggs. According to the artificial propagation method of the longfin cheilinus comatus, the indoor pool is adopted for cultivation, and high-quality backup fish is obtained through screening; and meanwhile, nutrition strengthening and strengthening cultivation management are carried out on the backup breeding fish, gonad development of the breeding fish is promoted, the cultivation mode of the breeding fish is optimized, the spawning induction medicament and dosage of the breeding fish are optimized and determined, and artificial ripening and spawning induction of the long-fin photopcheilium is realized.
Description
Technical Field
The application relates to the technical field of artificial propagation of fishes, in particular to an artificial propagation method of longfin cheilippine.
Background
Alcheilus longifolius (Acrossocheilusiridescenslongipinnis) belongs to Alcheilus genus of the family Cyprinus of the order Cyprinus. The special fish in the Zhujiang river basin are only distributed in the branches of the red water river in subtropical areas. The long-fin optical lip fish is very sensitive to environmental changes, and is frightened in the field fishing and transportation processes, so that the long-fin optical lip fish can die.
Because the natural distribution of the long-fin optical lip fish is limited, the influence of excessive fishing, ecological environment deterioration and the like, wild natural resources of the longfin fish are gradually deficient, the letter is to be protected. Therefore, the development of the cultivation, cultivation and artificial propagation of the longfin cheilis is not enough.
Disclosure of Invention
The embodiment of the application provides an artificial propagation method of longfin cheilinus, which aims to solve the problems of the related technology and has the following technical scheme:
the embodiment of the application provides an artificial propagation method of longfin cheilinus comatus, which comprises the following steps:
Selecting wild longfin cheilinus cinereus of proper age and weight of 350-500 g in advance for gradient domestication and feeding;
Placing the longfin cheilinus comatus into an indoor circulating water culture pond for culture to obtain the fish to be subjected to reinforced induced spawning;
carrying out intensified cultivation and cultivation on the obtained spawning-accelerating spawning-waiting fish to promote gonad development of the spawning fish;
Screening the breeding fish subjected to the intensified cultivation, and then carrying out anesthesia treatment on the screened breeding fish;
injecting an oxytocic into the anesthetized breeding fish;
Placing parent fish injected with the oxytocic into an oxytocic pool for artificial oxytocic fertilization;
hatching fertilized eggs, and hatching the fertilized eggs in a dark place after an eye bag period.
In one embodiment, the gradient domesticated feeding comprises:
Temporarily culturing the wild growth fin cheilinus edodes in a local culturing farm after capturing the river, extracting river water of a native place, simulating the native place and environment of the native place, and carrying out running water culture and domestication on the artificial pellet feed;
after the wild longfin cheilinus fasciatus domesticates the artificial pellet feed, changing the wild longfin cheilinus fasciatus domesticating artificial pellet feed into cement pond running water culture, and continuously domesticating the artificial pellet feed;
stopping running water culture after the artificial pellet feed is domesticated in the cement pond of the wild longfin fish, changing the cement pond still water culture into transportation after the wild longfin cheilinus comatus completely domesticates the artificial pellet feed;
The transportation is kept at low temperature and pure oxygen, and the wild growth fin cheilinus cinereus is completely domesticated at the moment and can be used as a parent.
In one embodiment, the subjecting the resulting spawning-waiting fish to enhanced cultivation, promoting gonadal development of the spawning fish, comprises: feeding sturgeon pellet feed according to 3% of the weight every day, and mixing and taking composite multidimensional; two months before parturition, mixing with tocopherol to promote gonadal development; the feeding amount is halved 15 days before the parturition.
In one embodiment, the selection criteria for screening the breed fish subjected to the enhanced culture are: female fish weight 350-500g, abdomen enlargement, lower abdomen softness, and diarrhea Kong Gongrun g, male fish weight 400-500g, kissing point star, body with frosty feel, light pressing abdomen with white semen outflow.
In one embodiment, the oxytocin in the oxytocin comprises chorionic gonadotrophin, luteinizing hormone No. 3, and diurone maleate.
In one embodiment, the dosage of oxytocin is 1500IU/kg of chorionic gonadotrophin, luteinizing hormone No. 3, 10ug/kg, diurone maleate 5mg/kg, based on body weight of the fish;
The oxytocic is prepared from oxytocic hormone and sodium chloride solution with the mass fraction of 0.7%; the injection dosage of the oxytocic is 0.8mL of the injection quantity of each female fish, the injection quantity of the male fish is halved, and the injection mode is secondary injection: the oxytocic is injected into the fish body twice, the first needle is used for injecting female fish only, the total dosage is 10% -20%, and the rest dosage is injected into the parent fish body for the second time.
In one embodiment, the step of placing the parent fish injected with the oxytocic into the oxytocic cell for artificial oxytocic fertilization comprises: placing parent fish injected with the oxytocic into an oxytocic tank, increasing the flushing water to increase the water flow rate of the oxytocic tank, adopting artificial spawning and semen collection after the effect time is reached, completing artificial insemination, wherein the male-female ratio is 2:1.
In one embodiment, the artificial insemination method employs a dry insemination method: the female fish is fished out and drained, the towel is used for wiping off the residual water on the surface of the fish, eggs are squeezed into a water wiping basin, a plurality of semen is squeezed into the basin, the basin is stirred uniformly, clear water is added into the basin, the eggs are fertilized by stirring for 2-3 minutes, and finally the basin is rinsed for 2 times.
In one embodiment, the incubating the fertilized egg after the eye bag period with light protection comprises: normally hatching by adopting a hatching device before membrane outlet; after the membrane is removed, after the development of the eye bag period, stopping the function of rolling fertilized eggs of the incubator, and covering a shading net; the whole hatching process uses running water for hatching.
In one embodiment, the incubator is a You Xianke incubator.
The advantages or beneficial effects in the technical scheme at least comprise:
(1) According to the artificial propagation method of the longfin cheilinus comatus, the indoor pool is adopted for cultivation, and high-quality backup fish is obtained through screening; and meanwhile, nutrition strengthening and strengthening cultivation management are carried out on the backup breeding fish, gonad development of the breeding fish is promoted, the cultivation mode of the breeding fish is optimized, and the spawning induction medicament and dosage of the breeding fish are optimized and determined, so that artificial ripening induction and spawning induction of the long-fin photopcheilium are realized.
(2) The gonad development of the male parent and the female parent is basically synchronous through artificial regulation and control of environmental factors, so that the smooth progress of artificial induced spawning is ensured, the method is simple, the operation is simple and convenient, and the practical applicability is good.
(3) Has stable catalytic rate and hatching rate: under natural conditions, female individual gonads of the long-fin photopcheilium are developed earlier than those of male fish, the inter-individual differences are obvious, the gonads are not developed synchronously, and large-scale breeding is difficult. The application combines the biological and reproductive ecological characteristics of the longfin cheilinus comatus, adopts the artificial regulation and control technology to carry out artificial induced spawning and breeding, the induced spawning rate reaches 70.8 percent, the fertilization rate reaches 87.2%, the emergence rate reaches 74.05%, and the method is completely suitable for large-scale production of fries, so that the method is used for protecting germplasm resources of the longfin cheilis and recovering natural populations of the longfin cheilis.
(4) Has stronger operability: the application solves the problem of asynchronous male and female development through a plurality of manual regulation and control technologies, ensures the smooth progress of artificial breeding, and has relatively simple method and strong operability.
(5) The artificial propagation of the seedling culture has the advantages of: 1) The fries are strong and symmetrical in physique, high in survival rate and high in bait utilization rate; 2) The growth speed is faster than that of a wild individual, and the method has good adaptability in natural environment; 3) The fish fry has strong disease resistance, is easy to be domesticated manually, can reach 6-8cm after 3-4 months of cultivation, meets the manual release requirement, and is favorable for maintaining natural population and recovery and the aquaculture and fishery cultivation scale.
The foregoing summary is for the purpose of the specification only and is not intended to be limiting in any way. In addition to the illustrative aspects, embodiments, and features described above, further aspects, embodiments, and features of the present application will become apparent by reference to the drawings and the following detailed description.
Drawings
In the drawings, the same reference numerals refer to the same or similar parts or elements throughout the several views unless otherwise specified. The figures are not necessarily drawn to scale. It is appreciated that these drawings depict only some embodiments according to the disclosure and are not therefore to be considered limiting of its scope.
FIG. 1 shows a long fin lipfish fry bred by the method of the present application.
Detailed Description
Hereinafter, only certain exemplary embodiments are briefly described. As will be recognized by those of skill in the pertinent art, the described embodiments may be modified in various different ways without departing from the spirit or scope of the present application. Accordingly, the drawings and description are to be regarded as illustrative in nature and not as restrictive.
The embodiment of the application provides an artificial propagation method of longfin cheilinus comatus, which comprises the following steps:
S1, screening the breeding fish.
And selecting wild longfin cheilinus cinereus with proper age and weight of 350-500 g for gradient domestication feeding one year in advance.
Because the long-fin optical lip fish is very sensitive to environmental changes and is frightened in the field fishing and transportation processes, the long-fin optical lip fish can die, and therefore, the domestication of the long-fin optical lip fish adopts a gradient domestication method.
In one embodiment, the gradient domesticated feeding comprises:
Temporarily culturing the wild growth fin cheilinus edodes in a local culturing farm after capturing the river, extracting river water of a native place, simulating the native place and environment of the native place, and carrying out running water culture and domestication on the artificial pellet feed;
after the wild longfin cheilinus fasciatus domesticates the artificial pellet feed, changing the wild longfin cheilinus fasciatus domesticating artificial pellet feed into cement pond running water culture, and continuously domesticating the artificial pellet feed;
stopping running water culture after the artificial pellet feed is domesticated in the cement pond of the wild longfin fish, changing the cement pond still water culture into transportation after the wild longfin cheilinus comatus completely domesticates the artificial pellet feed;
The transportation is kept at low temperature and pure oxygen, and the wild growth fin cheilinus cinereus is completely domesticated at the moment and can be used as a parent.
S2, indoor cultivation of the breeding fish.
And (5) placing the longfin cheilinus comatus into an indoor circulating water culture pond for culture to obtain the fish to be subjected to reinforced induced spawning.
S3, reinforcing and maturing-promoting cultivation of the breeding fish.
And (3) carrying out intensified cultivation and cultivation on the obtained spawning-accelerating spawning-waiting fish, and promoting gonad development of the spawning fish.
In one embodiment, the step S3 includes: feeding sturgeon No. 2 pellet feed according to 3% of the weight every day, and mixing and taking composite multidimensional; two months before parturition, mixing with tocopherol to promote gonadal development; the feeding amount is halved 15 days before the parturition.
S4, selecting and anaesthetizing the breeding fish.
And screening the breeding fish subjected to the intensified cultivation, and then carrying out anesthesia treatment on the screened breeding fish.
In one embodiment, the selection criteria for screening the breed fish subjected to the enhanced culture are: female fish weight 350-500g, abdomen enlargement, lower abdomen softness, and diarrhea Kong Gongrun g, male fish weight 400-500g, kissing point star, body with frosty feel, light pressing abdomen with white semen outflow.
S5, spawning induction of the fish.
Injecting oxytocic into the anesthetized fish.
In one embodiment, the oxytocin in the oxytocin comprises chorionic gonadotrophin, luteinizing hormone No. 3, and diurone maleate.
In one embodiment, the dosage of oxytocin is 1500IU/kg of chorionic gonadotrophin (HCG), 10ug/kg of luteinizing hormone number 3 (LRH-A3), 5mg/kg of diurone maleate (DOM), based on the weight of the fish body;
The oxytocic is prepared from oxytocic hormone and sodium chloride solution with the mass fraction of 0.7%; the injection dosage of the oxytocic is 0.8mL of the injection quantity of each female fish, the injection quantity of the male fish is halved, and the injection mode is secondary injection: the oxytocic is injected into the fish body twice, the first needle is used for injecting female fish only, the total dosage is 10% -20%, and the rest dosage is injected into the parent fish body for the second time.
S6, artificial insemination.
And (5) placing the parent fish injected with the oxytocic into an oxytocic pool for artificial oxytocic fertilization.
In one embodiment, the step of placing the parent fish injected with the oxytocic into the oxytocic cell for artificial oxytocic fertilization comprises: placing parent fish injected with the oxytocic into an oxytocic tank, increasing the flushing water to increase the water flow rate of the oxytocic tank, adopting artificial spawning and semen collection after the effect time is reached, completing artificial insemination, wherein the male-female ratio is 2:1.
In one embodiment, the artificial insemination method employs a dry insemination method: the female fish is fished out and drained, the towel is used for wiping off the residual water on the surface of the fish, eggs are squeezed into a water wiping basin, a plurality of semen is squeezed into the basin, the basin is stirred uniformly, clear water is added into the basin, the eggs are fertilized by stirring for 2-3 minutes, and finally the basin is rinsed for 2 times.
S7, hatching.
Hatching fertilized eggs, and hatching the fertilized eggs in a dark place after an eye bag period.
In one embodiment, the step S7 includes: normally hatching by adopting a hatching device before membrane outlet; after the membrane is removed, after the development of the eye bag period, stopping the function of rolling fertilized eggs of the incubator, and covering a shading net; the whole hatching process uses running water for hatching.
In one embodiment, the incubator is a You Xianke incubator.
Propagation cases
And 101, screening the breeding fish.
And selecting wild longfin cheilinus cinereus with proper age and weight of 350-500 g for gradient domestication feeding one year in advance.
And 102, indoor cultivation of the breeding fish. The area of the parent fish culture pond is about 80m 2, the water depth is about 2m, and the parent fish is fed in running water; feeding sturgeon feed with 48% of protein content. The daily feeding amount is 2-3% of the weight of the fish, the fish is fed once in the morning and at night, and the feeding amount is reduced to half of the original feeding amount 15 days before artificial propagation.
Step 103, reinforcing and maturing the breeding fish.
Carrying out reinforced cultivation on the obtained fish to be reinforced and induced spawning, feeding sturgeon No. 2 pellet feed according to 3% of the weight every day, and mixing and taking composite multidimensional; two months before parturition, mixing with tocopherol to promote gonadal development; the feeding amount is halved 15 days before the parturition.
Step 104, the breeding fish is selected and anesthetized.
Screening the breed fish subjected to the intensified culture, wherein the screening standard is as follows: the weight of the male fish is 400-500g, the kissing part is rough, the body has granular feel, the body color is gorgeous, and the base part of the lightly extruded ventral fin has white semen to flow out; female fish weight is 350g-400g, ovary is well developed, the upper abdomen is enlarged, the lower abdomen is soft, and the female fish is colonised Kong Gongrun. According to 2:1, and selecting the male-female ratio. Anesthesia is then performed.
Step 105, spawning induction of the spawning fish.
Injecting an oxytocic into the anesthetized breeding fish, and then placing the breeding fish into an oxytocic pool for artificial oxytocic fertilization; oxytocin in the oxytocin includes chorionic gonadotrophin HCG, luteinizing hormone number 3 (LRH-A3) and dioleyl maleate (DOM); the oxytocin dose is 1500IU/kg of chorionic gonadotrophin (HCG), 10ug/kg of luteinizing hormone releasing hormone number 3 (LRH-A3), 5mg/kg of diurone maleate (DOM), and calculated according to the weight of the fish body; the oxytocic is prepared from oxytocic hormone and sodium chloride solution with the mass fraction of 0.7%; the injection dosage of the oxytocic is 0.8mL of the injection quantity of each female fish, the injection quantity of the male fish is halved, and the injection mode is secondary injection: the oxytocic is injected into the fish body twice, the first needle is used for injecting female fish only, the total dosage is 10% -20%, and the rest dosage is injected into the parent fish body for the second time. The method for injecting the oxytocic into the parent fish is that the base of the pectoral fin of the injector is injected, the specific position is that the depression at the rear side is inclined by 45 degrees and injected into the abdominal cavity, and the injection depth of the injector is mm. The injection mode is secondary injection: the oxytocic is injected into the fish body twice, the first needle is used for injecting female fish only, the total dosage is 10% -20%, and the rest dosage is injected into the parent fish body for the second time.
And 106, artificial insemination.
Placing the injected parent fish into an induced spawning pond, increasing the water flow rate of the induced spawning pond by increasing the water flushing, and after the effect time is reached, carrying out spawning and semen collection when the parent fish is in oestrus and climbs and begins to spawn, and completing artificial insemination to form fertilized eggs.
Step 107, hatching.
The artificial hatching is carried out by adopting a You Xianke incubator, and the whole hatching process is divided into two stages.
The specific hatching method comprises the following steps: before the membrane is removed, normally hatching by adopting a You Xianke incubator; after the membrane is removed, after the development of the eye bag period, the function of rolling fertilized eggs of the You Xianke incubator is stopped, and a shading net is covered. The whole hatching process uses running water for hatching. Referring to fig. 1, fig. 1 shows a long fin lipfish fry bred by the method of the present application.
Before the membrane is removed, fertilized eggs are extremely easy to infect the saprolegnia, if the fertilized eggs are not picked up in time, a plurality of fertilized eggs are stuck together to infect the saprolegnia, so that the hatching rate is greatly reduced, and the white eggs and the mould eggs need to be picked up at least twice per day before the membrane is removed.
Specific propagation information is shown in Table 1 below.
TABLE 1
In the description of the present specification, a description referring to terms "one embodiment," "some embodiments," "examples," "specific examples," or "some examples," etc., means that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the present application. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples. Furthermore, the different embodiments or examples described in this specification and the features of the different embodiments or examples may be combined and combined by those skilled in the art without contradiction.
Furthermore, the terms "first," "second," and the like, are used for descriptive purposes only and are not to be construed as indicating or implying a relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defining "a first" or "a second" may explicitly or implicitly include at least one such feature. In the description of the present application, the meaning of "a plurality" is two or more, unless explicitly defined otherwise.
Any process or method description in a flowchart or otherwise described herein may be understood as representing modules, segments, or portions of code which include one or more executable instructions for implementing specific logical functions or steps of the process. And the scope of the preferred embodiments of the present application includes additional implementations in which functions may be performed in a substantially simultaneous manner or in an opposite order from that shown or discussed, including in accordance with the functions that are involved.
Logic and/or steps represented in the flowcharts or otherwise described herein, e.g., a ordered listing of executable instructions for implementing logical functions, can be embodied in any computer-readable medium for use by or in connection with an instruction execution system, apparatus, or device, such as a computer-based system, processor-containing system, or other system that can fetch the instructions from the instruction execution system, apparatus, or device and execute the instructions.
It is to be understood that portions of the present application may be implemented in hardware, software, firmware, or a combination thereof. In the above-described embodiments, the various steps or methods may be implemented in software or firmware stored in a memory and executed by a suitable instruction execution system. All or part of the steps of the methods of the embodiments described above may be performed by a program that, when executed, comprises one or a combination of the steps of the method embodiments, instructs the associated hardware to perform the method.
In addition, each functional unit in the embodiments of the present application may be integrated in one processing module, or each unit may exist alone physically, or two or more units may be integrated in one module. The integrated modules may be implemented in hardware or in software functional modules. The integrated modules described above, if implemented in the form of software functional modules and sold or used as a stand-alone product, may also be stored in a computer-readable storage medium. The storage medium may be a read-only memory, a magnetic or optical disk, or the like.
The foregoing is merely illustrative of the present application, and the present application is not limited thereto, and any person skilled in the art will readily recognize that various changes and substitutions are possible within the scope of the present application. Therefore, the protection scope of the application is subject to the protection scope of the claims.
Claims (8)
1. An artificial propagation method of longfin cheilippines, comprising the steps of:
Selecting wild longfin cheilinus cinereus of proper age and weight of 350-500 g in advance for gradient domestication and feeding;
Placing the longfin cheilinus comatus into an indoor circulating water culture pond for culture to obtain the fish to be subjected to reinforced induced spawning;
carrying out intensified cultivation and cultivation on the obtained spawning-accelerating spawning-waiting fish to promote gonad development of the spawning fish;
Screening the breeding fish subjected to the intensified cultivation, and then carrying out anesthesia treatment on the screened breeding fish;
injecting an oxytocic into the anesthetized breeding fish;
Placing parent fish injected with the oxytocic into an oxytocic pool for artificial oxytocic fertilization;
hatching fertilized eggs, and hatching the fertilized eggs in a dark place after an eye bag period;
wherein the gradient domestication feeding comprises:
Temporarily culturing the wild growth fin cheilinus edodes in a local culturing farm after capturing the river, extracting river water of a native place, simulating the native place and environment of the native place, and carrying out running water culture and domestication on the artificial pellet feed;
after the wild longfin cheilinus fasciatus domesticates the artificial pellet feed, changing the wild longfin cheilinus fasciatus domesticating artificial pellet feed into cement pond running water culture, and continuously domesticating the artificial pellet feed;
stopping running water culture after the artificial pellet feed is domesticated in the cement pond of the wild longfin fish, changing the cement pond still water culture into transportation after the wild longfin cheilinus comatus completely domesticates the artificial pellet feed;
The transportation is kept at low temperature and pure oxygen, and the wild growth fin cheilinus comatus is completely domesticated at the moment and can be used as a parent;
the hatching of fertilized eggs after the eye bag period adopts light-shielding hatching, and comprises the following steps: before membrane emergence, normally incubating by adopting an incubator, and picking off white eggs and mould eggs at least twice a day; after the membrane is removed, after the development of the eye bag period, stopping the function of rolling fertilized eggs of the incubator, and covering a shading net; the whole hatching process uses running water for hatching.
2. The artificial propagation method of longfin cheilinus comatus according to claim 1, wherein the step of performing the intensive cultivation and cultivation of the obtained species fish to be induced spawning to promote gonad development of the species fish comprises the steps of: feeding sturgeon pellet feed according to 3% of the weight every day, and mixing and taking composite multidimensional; two months before parturition, mixing with tocopherol to promote gonadal development; the feeding amount is halved 15 days before the parturition.
3. The artificial propagation method of longfin cheilinus comatus according to claim 1, wherein the selection criteria for screening the bred fish subjected to intensive cultivation are: female fish weight 350-500g, abdomen enlargement, lower abdomen softness, and diarrhea Kong Gongrun g, male fish weight 400-500g, kissing point star, body with frosty feel, light pressing abdomen with white semen outflow.
4. The artificial propagation method of longfin lipfish according to claim 1, wherein the oxytocin in the oxytocin comprises chorionic gonadotrophin, luteinizing hormone No. 3 and diurone maleate.
5. The artificial propagation method of longfin lipfish according to claim 4, wherein the dosage of oxytocin is 1500IU/kg of chorionic gonadotrophin, 10ug/kg of luteinizing hormone No.3, 5mg/kg of diurone maleate, based on the weight of the fish body;
The oxytocic is prepared from oxytocic hormone and sodium chloride solution with the mass fraction of 0.7%; the injection dosage of the oxytocic is 0.8mL of the injection quantity of each female fish, the injection quantity of the male fish is halved, and the injection mode is secondary injection: the oxytocic is injected into the fish body twice, the first needle is used for injecting female fish only, the total dosage is 10% -20%, and the rest dosage is injected into the parent fish body for the second time.
6. The artificial propagation method of longfin cheilippines according to claim 1, wherein the step of placing the parent fish injected with the oxytocic into an oxytocic cell for artificial oxytocic fertilization comprises: placing parent fish injected with the oxytocic into an oxytocic tank, increasing the flushing water to increase the water flow rate of the oxytocic tank, adopting artificial spawning and semen collection after the effect time is reached, completing artificial insemination, wherein the male-female ratio is 2:1.
7. The artificial propagation method of longfin cheilinus comatus according to claim 6, wherein the artificial fertilization method adopts a dry fertilization method: the female fish is fished out and drained, the towel is used for wiping off the residual water on the surface of the fish, eggs are squeezed into a water wiping basin, a plurality of semen is squeezed into the basin, the basin is stirred uniformly, clear water is added into the basin, the eggs are fertilized by stirring for 2-3 minutes, and finally the basin is rinsed for 2 times.
8. The artificial propagation method of longfin cheilis according to any one of claims 1-7, wherein the incubator is a You Xianke incubator.
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