CN101647412B - Method for breeding tridentiger trigonocephalus by artificial induced spawning and insemination - Google Patents
Method for breeding tridentiger trigonocephalus by artificial induced spawning and insemination Download PDFInfo
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- CN101647412B CN101647412B CN2008100415729A CN200810041572A CN101647412B CN 101647412 B CN101647412 B CN 101647412B CN 2008100415729 A CN2008100415729 A CN 2008100415729A CN 200810041572 A CN200810041572 A CN 200810041572A CN 101647412 B CN101647412 B CN 101647412B
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Abstract
The invention provides a method for breeding tridentiger trigonocephalus by artificial induced spawning and insemination, which relates to the artificial propagation of gobies. The method required to be provided for artificially breeding the tridentiger trigonocephalus comprises the steps of ripe fish screening, maturity acceleration, insemination, incubation and larval fish culture, wherein the maturity acceleration of ripe fishes uses luteotropin to release hormone analogues, domperidone maleate and chorionic gonadotropin, and is characterized in that the three hormones are used twice for the maturity acceleration; in the maturity acceleration, the water temperature of culture is between 18 and 21 DEG C, the salinity is between 2 and 6, the dissolved oxygen is between 3.5 and 6.5mg/L, and the pH is between 7.5 and 9.0; and after the second time of the maturity acceleration, in 8 to 10 hours, mature ova and mature sperms are selected for artificial insemination which comprises the following steps that: the activated sperms are poured on the ova for sufficient contact so as to obtain fertilized ova; the fertilized ova are placed in a hatchery pond for incubation; and the hatched larval fish is placed in a culture pond for incubation, and chlorella and yolk powder of eggs are placed in water from the third day after the larval fish is hatched to feed food for the larval fish. The method is used for the artificial breeding of the tridentiger trigonocephalus.
Description
Technical field:
The present invention relates to the artificial fecundation method of goby section fish.
Background technology
The artificial propagation of fish method mainly comprises 3 kinds: 1. have an injection and hasten parturition: manual injection's medicament of hastening parturition that accelerates the ripening makes the laggard pedestrian worker's insemination of the smart maturation of ovum; 2. directly carry out artificial insemination: after the parent population of catching is screened, directly take out sperm and ovum and carry out artificial insemination; 3. ecological breeding method: parent population is put into the breeding box of artificial regulatory ecological factor, make its physical maturity, oestrus, lay eggs, be fertilized, hatch.The method of hastening parturition of having an injection during artificial breeding fish seed is by the specific exogenous hormone of regular injection, promote the fish maturation and lay eggs, take specific artificial insemination method after arriving effector phase, mix sperm and ovum and obtain fertilized egg, development of fertilized ova becomes the embryo, until the prelarva membrane, prelarva ingests after the certain openings bait gradually that development growth is a marketable fish in specific environment of hatching.The way that artificial induced spawning and insemination is bred the fish seed seems simple, but every kind of fish of natural world have all experienced very long evolutionary history, all formed and bred characteristics separately, therefore when artificial propagation, the artificial breeding method of creativity and innovation must be fit to the Physiological Ecology characteristics of fish separately, could succeed.The artificial breeding method of present many fish seeds is success not yet, still need rely on when propagating artificially and fish for wild seed.Artificial breeding method as the common eel seed, though the manpower financial capacity that lot of domestic and foreign unit's input is a large amount of, comprise that China also studies in the past a plurality of " countries 5 years emphasis key scientific and technological projects ", some progress have also been obtained, but the artificial breeding method of common eel seed does not succeed yet fully at present, and common eel is cultured to be still by fishing for the eel seedling every year and keeps.The mating system that many fish seeds are certainly also arranged through big quantity research, is invented successfully finally, and has been applied for patent of invention, as dark line globefish, Cynoglossus semilaevis, mudskipper,
Fish, schizothoracin, erythroculter ilishaeformis, Odontobutis obscura, sweetfish etc.
The goby class is of a great variety, and Liu Qun etc. have 7 sections report whole world goby class in 1997,263 genus, and about about 1720 kinds, wherein only just there are 200 genus in goby section, at least 1500 kinds more than, becomes maximum in a fish section.Goby flying fish generic Perciformes, goby suborder, various types of between external form similar, no eyelid, two in dorsal fin does not link to each other, left and right sides abdomeinal fin heals into the sucker shape.Goby section fish are widely distributed, and fresh water, seawater kind all have, and some gobies live in degree of saltiness water waters, and salinity accommodation is wider.Because goby section fish are wild kind mostly, economic worth has certain limitation, and therefore the research of prawn Gobiidae fish both at home and abroad deeply seldom.The reproductive biology of prawn Gobiidae research at present mainly comprises reports such as individual reproduction power, reproductive habit and colony form, the early stage history of life.Report about goby section fish seed breeding, Zhao Bin, Fan Liping, people such as Wang Jian are in the breeding situation of the multiple goby of report lance tail on " fishery modernization " periodical in 2005, the main multiple goby parent population of lance tail that relies on natural maturation, in the pond natural spawning and hatch fry after, the bait such as soya-bean milk of manually throwing something and feeding are fished for the fry in the pond then.Their propagation technique is the propagation technique under the natural conditions, and its parent is the parent population of physical maturity in the natural surroundings.
The line thin white silk used in ancient China
Tiger fish (Tridentiger trigonocephalus) belongs to Perciformes (Perciformes)
Gobiidae (Gobiidae) thin white silk used in ancient China
The tiger fish belongs to (Tridentiger), and this fish happiness is lived in caves, and is food with shrimp, small fish etc., and the male and female individuality has notable difference.The line thin white silk used in ancient China
The tiger fish is littoral eurysalinity benthic fishes, mainly is distributed in the pacific rim of northeast, Asia, is mainly seen in waters such as the yellow Bohai Sea, the East Sea, the South Sea and the Taiwan Straits in China, also is the common small fishes in low salinity zone, entrance of Changjiang River.The line thin white silk used in ancient China
The breeding period of tiger fish is longer, and maturity of fish gonads disunity, sperm and ovum exist grows nonsynchronous problem, and raun is more in the parent population of catching, and milter is less.Do not see relevant line thin white silk used in ancient China at present
Tiger fry kind artificial breeding method's report.
Line thin white silk used in ancient China goby is as one of key species of entrance of Changjiang River aquatic ecosystem; in flowing, the material Transfer of the ecosystem and energy have important function; also be one of topmost food organisms of entrance of Changjiang River mandarin sturgeon juvenile fish; therefore invention line thin white silk used in ancient China goby fingerlings artificial mating system has great importance for the sustainable development of entrance of Changjiang River ecotope and the protection of mandarin sturgeon species.
Summary of the invention
The object of the invention is at tridentiger trigonocephalus with artificial breeding and the existing problem of seedling growing process, the offspring breeding method that provides a kind of tridentiger trigonocephalus with artificial to hasten parturition insemination.
Technical scheme of the present invention by parent population screen, accelerate the ripening, artificial insemination, hatching, prelarva cultivates successively in conjunction with forming, at first the ratio by parent population head form and scalability shape carries out male and female and identifies that (the wide flat and cheek muscle of head is outstanding to be the milter feature, and the head triangle is the raun feature; Male fish length/height, head length/eye footpath and head length/kiss are long significantly greater than raun, and body length/head length, head length/eye spacing are significantly less than female parent population) screen also that (pectoral fin of milter is glassy yellow, and obvious black splotch is arranged on pectoral fin; The raun belly expands outstanding, and the gonopore evagination takes on a red color) grow parent population preferably; The accelerate the ripening hormone of injection of parent population is luteotropin releasing hormone d-ala analog (LHRH-A
2), maleic acid DOM (DOM) and human chorionic gonadtropin (HCG), 3 kinds of hormones mix with physiological saline solution, it is characterized in that: the hormone dosage that per kilogram fish body weight is used is LHRH-A
2The use (female milter dosage is identical) of accelerating the ripening at twice of 10 micrograms, DOM4.8 milligram, these 3 kinds of hormone dosages of HCG1600IU (international unit), interval time is 12 hours, accelerates the ripening for the 1st time to use LHRH-A
24 micrograms, DOM4.8 milligram and 1 ml physiological saline, the 2nd the use LHRH-A that accelerates the ripening
26 micrograms, HCG1600IU and 1 ml physiological saline; The breeding environment condition is controlled at 18-21 ℃ of water temperatures when accelerating the ripening, salinity 2-6, dissolved oxygen 3.5-6.5mg/L, pH7.5～9.0; After accelerating the ripening for the 2nd time, under 20 ℃ of conditions of water temperature, promptly squeezed ovum and squeezed essence through 8-10 hours, extrude female fish egg earlier and check its maturity, choose the ovum circle and be yellow, the oil ball is more and have a better glossiness, the mature egg of the sticking string test prosperity in the egg membrane outside is used for artificial insemination: check the spermioteleosis degree then, choose that spermary is full to be creamy white, the mature sperm that the spermary surface is full of seminal fluid is used for artificial insemination: vigor was more than 80% after sperm was met water, the sperm that activates is watered on ovum, allow ovum fully contact fertilization with the tweezers stirring, promptly obtain fertilized egg with sperm; It is online that fertilized egg is placed on bolting silk, places the hatchery to hatch, at the bottom of the pond, hatchery and pool wall lay black ceramic tiles; Incubation condition is controlled at 17-21 ℃ of temperature, whenever round the clock more than the illumination 12h, and pH7.5-9.5, salinity is 4-6, more than the dissolved oxygen 6mg/L, keeps the flow velocity of water body to maintain below 1 cel, can rupture of membranes and go out through 150-200 hours prelarvas; The prelarva that hatches placed cultivate the pond and hatch, breeding condition is 18-21 ℃ of temperature, every illumination 12h round the clock, and pH7.5-9.5, salinity is 2-4, beginning in the 3rd day is put into the yolk powder of chlorella and egg for its initial feeding (line thin white silk used in ancient China in water behind the prelarva membrane
The outside food of can ingesting during tiger fish prelarva 3-4 ages in days, grow enter exogenous nutrition and endogenous nutrition mixing period), the early stage prelarva developmental stage is finished in prelarva yolk sac complete obiteration during 5 ages in days.
Advantage of the present invention shows the hormone appropriate dosage and the method for effectively hastening parturition of selecting parent population to hasten parturition required, utilize the physiology and the ecosystem characterization of the reproduction of line thin white silk used in ancient China goby, selection can obtain the mature egg of high fertilization rate, and adopt endocrine regulation and simulate the method for reproduction environment, pass through artificial insemination method, greatly improve the rate of accelerating the ripening, fertilization rate and the emergence rate of parent population, made the tridentiger trigonocephalus with artificial breeding succeed the good effect of making a breakthrough property first.
Embodiment
The present invention's operation is undertaken by following order:
1. parent population screening: the line thin white silk used in ancient China that natural water area is fished for
The tiger fish parent fish, the breeding spot is transported in oxygenation back.At first carrying out male and female identifies, the wide flat and cheek muscle of head is outstanding to be the milter feature, the head triangle is the raun feature, externally can directly distinguish the parent population sex by perusal parent population nose shape on the form, for selecting of female milter provides authentication method fast.There is utmost point significant difference between male and female parent fish length/height, body length/head length, head length/eye footpath and the head length/eye spacing in addition, head length/kiss lives forever in significant difference, wherein male fish length/height, head length/eye footpath and head length/kiss are long significantly greater than raun, and body length/head length, head length/eye spacing be significantly less than female parent population, and the ratio by form scalability shape can also carry out male and female easily and identify (seeing Table 1).After male and female were identified, screening grew preferably that parent population breeds, and the belly that screening is characterized as raun expands outstanding, and the gonopore evagination takes on a red color; The pectoral fin of milter is glassy yellow, and obvious black splotch is arranged on pectoral fin.
Female, the male parent population morphology of table 1 line thin white silk used in ancient China goby scalability shape ratio value
2. parent population is accelerated the ripening: put into 20 pairs of line thin white silks used in ancient China in 5 square metres of cement pits
The tiger fish parent fish is promptly put into the female line thin white silk used in ancient China of 20 tails
The male line thin white silk used in ancient China of tiger fish parent fish and 20 tails
The tiger fish parent fish.The cave that has 25 tiles etc. to build up in the cement pit can supply the line thin white silk used in ancient China
The tiger fish parent fish pierces wherein, and the control of breeding environment condition is as follows when accelerating the ripening: 18-21 ℃ of water temperatures, salinity 2-6, dissolved oxygen 3.5-6.5mg/L, pH7.5～9.0.Support 1 all stable backs temporarily and adopt hormone to accelerate the ripening, the hormone of employing is luteotropin releasing hormone d-ala analog (LHRH-A
2), maleic acid DOM (DOM) and human chorionic gonadtropin (HCG), 3 kinds of hormones mix with physiological saline solution, the hormone dosage that adopts for per kilogram fish body weight is LHRH-A
210 micrograms, DOM4.8 milligram, HCG1600IU (international unit), female milter dosage is identical, and above-mentioned dosage is divided into twice and accelerates the ripening, and interval time is 12 hours, and the 1st time the dosage that accelerates the ripening is LHRH-A
24 micrograms, DOM4.8 milligram and 1 ml physiological saline, the 2nd time the dosage that accelerates the ripening is LHRH-A
26 micrograms, HCG1600IU and 1 ml physiological saline.
3. artificial insemination: after parent population is accelerated the ripening, under 20 ℃ of conditions of water temperature, can squeeze ovum and squeeze essence through 8-10 hours.Adopt semi-dry process to carry out artificial insemination.Extrude female fish egg earlier and check its maturity, choose the mature egg with following feature and be used for artificial insemination: the ovum circle is yellow; The oil ball is more and have a glossiness; The adhesive filament prosperity of the egg membrane outside.Check the spermioteleosis degree then, choose the mature sperm with following feature and be used for artificial insemination: spermary is full to be creamy white; The spermary surface is full of seminal fluid; Vigor was more than 80% after sperm was met water.The test-tube operating process of semi-dry process is as follows: get female fish ovaries, in culture dish, ovum is separated with tweezers, get the milter spermary simultaneously, be cut into small pieces, after placing the interior grinding of mortar, add a little 4 ‰ sea-water activated sperm, immediately the sperm that activates is watered on ovum, allow ovum fully contact fertilization with the tweezers stirring, can obtain fertilized egg with sperm.Adopt such artificial insemination method to have higher fertilization rate (seeing Table 2-5)
4. incubating oosperm: it is online that fertilized egg is placed on bolting silk, places the hatchery to hatch, at the bottom of the pond, hatchery and pool wall lay black ceramic tiles.Incubation condition control is as follows: 17-21 ℃ of temperature, and whenever round the clock more than the illumination 12h, pH7.5-9.5, salinity is 4-6, more than the dissolved oxygen 6mg/L, keeps water body to have flow velocity slowly, below 1 cel.Can rupture of membranes and go out through 150-200 hours prelarvas.Under suitable like this environment, has higher incubation rate (seeing Table 2-5).
Fertilization rate, incubation rate and the development time of line thin white silk used in ancient China goby in table 2 different temperatures
Fertilization rate, incubation rate and the development time of line thin white silk used in ancient China goby in table 3 different illumination conditions
Fertilization rate, incubation rate and the development time of line thin white silk used in ancient China goby among the different pH of table 4
Fertilization rate, incubation rate and the brooding time of line thin white silk used in ancient China goby in table 5 different salinity
5. prelarva is cultivated: the prelarva that hatches is placed cultivate the pond and hatch, breeding condition control is as follows: 18-21 ℃ of temperature, and every illumination 12h round the clock, pH7.5-9.5, salinity is 2-4, has higher survival activity index (table 6-9) this condition eyeball prelarva.Beginning in the 3rd day is put into the yolk powder of chlorella and egg for its initial feeding in water behind the prelarva membrane.The line thin white silk used in ancient China of firm membrane
Tiger fish prelarva health is transparent, total length 2.534 ± 0.078mm, height 0.559 ± 0.053mm, yolk sac major diameter 0.421 ± 0.047mm, yolk sac minor axis 0.369 ± 0.038mm, the oil ball is bordering on the yolk sac front end partially, still be in endogenous nutrition period, the outside food of can ingesting during 3-4 ages in days is grown and is entered exogenous nutrition and endogenous nutrition mixing period, the early stage prelarva developmental stage is finished in prelarva yolk sac complete obiteration during 5 ages in days.
Survival rate and the survival activity index SAI of line thin white silk used in ancient China goby prelarva in the different temperatures when table 6 is hungry
Survival rate and the survival activity index SAI of line thin white silk used in ancient China goby prelarva under the different illumination conditions when table 7 is hungry
Survival rate and the survival activity index SAI of line thin white silk used in ancient China goby prelarva among the different pH when table 8 is hungry
Line thin white silk used in ancient China in different salinity when table 9 is hungry
Survival rate and the survival activity index SAI of tiger fish prelarva
Claims (1)
1. method for breeding tridentiger trigonocephalus by artificial induced spawning and insemination, by parent population screen, accelerate the ripening, artificial insemination, hatching, prelarva cultivates successively in conjunction with forming, at first carry out male and female and identify and screen and grow parent population preferably; The accelerate the ripening hormone of usefulness of parent population is luteotropin releasing hormone d-ala analog LHRH-A
2, maleic acid DOM (DOM) and human chorionic gonadtropin (HCG), it is characterized in that the hormone dosage that per kilogram fish body weight is used is LHRH-A
210 micrograms, 4.8 milligrams of DOM, HCG 1600 IU (international unit), these the 3 kinds of hormone dosages use of accelerating the ripening at twice, twice interval time is 12 hours, accelerates the ripening for the 1st time to use LHRH-A
24 micrograms, 4.8 milligrams of DOM and 1 ml physiological saline, the 2nd the use LHRH-A that accelerates the ripening
26 micrograms, HCG 1600 IU and 1 ml physiological saline; When accelerating the ripening temperature of cultivation 18-21 ℃, salinity 2-6, dissolved oxygen 3.5-6.5mg/L, pH 7.5～9.0; After accelerating the ripening for the 2nd time, under 20 ℃ of conditions of water temperature, promptly squeezed ovum and squeezed essence through 8-10 hour, extrude female fish egg earlier, choose the ovum circle and be yellow, the oil ball is more and have a better glossiness, the mature egg of egg membrane outside adhesive filament prosperity is used for artificial insemination, choose then that spermary is full to be creamy white, the mature sperm that the spermary surface is full of seminal fluid is used for artificial insemination, test-tube operating process is: get female fish ovaries, in culture dish, ovum is separated with tweezers, get the milter spermary simultaneously, be cut into small pieces, place grind in the mortar after, add a little 4 ‰ sea-water activated sperm, vigor was more than 80% after sperm was met water, the sperm that activates is watered on ovum, allow ovum fully contact fertilization, promptly obtain fertilized egg with sperm; It is online that fertilized egg is placed on bolting silk, places the hatchery to hatch, at the bottom of the pond, hatchery and pool wall lay black ceramic tiles; Incubation condition is controlled at temperature 17-21 ℃, and whenever round the clock more than the illumination 12h, pH 7.5～9.5, and salinity 4-6 more than the dissolved oxygen 6mg/L, keeps the flow velocity of water body to maintain below 1 cel, can rupture of membranes and go out through 150-200 hour prelarva; Place the cultivation pond to hatch the prelarva that hatches, breeding condition is at temperature 18-21 ℃, whenever illumination 12h round the clock, pH 7.5～9.5, salinity is 2-4, the yolk powder that chlorella and egg are put in beginning in the 3rd day in water behind the prelarva membrane is for its initial feeding, and the early stage prelarva developmental stage is finished in prelarva yolk sac complete obiteration during 5 ages in days.
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| CN102177863B (en) * | 2011-05-26 | 2012-10-24 | 浙江省淡水水产研究所 | Method for inducing hormone soaked loaches |
| CN102228681A (en) * | 2011-06-09 | 2011-11-02 | 吴江市水产养殖有限公司 | Fish spawning inducer and spawning induction method |
| CN102835340B (en) * | 2012-09-24 | 2013-08-21 | 厦门大学 | Breeding method of Rillengrundel |
| CN103404469B (en) * | 2013-08-26 | 2015-08-05 | 华能澜沧江水电有限公司 | The artificial fecundation method of a kind of Ju Po |
| CN103718998B (en) * | 2013-12-18 | 2018-04-27 | 上海市水产研究所 | A kind of method for obtaining Synechogobius ommaturus ecology early-breeding seedlings kind |
| CN104585093B (en) * | 2015-01-14 | 2016-08-31 | 成都市天府新区丹龙渔业养殖专业合作社 | A kind of method of artificial induced spawning fish-egg |
| CN107333680A (en) * | 2016-04-29 | 2017-11-10 | 岚皋县绿水生态农业有限责任公司 | A kind of artificial breeding method of more squama shovel jaw fishes and special hatchery |
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