CN103202391A - Compound lactobacillus probiotics for effectively preventing and improving bovine mastitis - Google Patents
Compound lactobacillus probiotics for effectively preventing and improving bovine mastitis Download PDFInfo
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Abstract
Disclosed is compound lactobacillus probiotics for effectively preventing and improving bovine mastitis. The compound lactobacillus probiotics is characterized by comprising, by mass, 10% of lactobacillus casei HM-09 (L.casei HM-09), 10% of lactobacillus plantarum HM-10(L.plantarum HM-10) and the rest 80% of zeolite powder, viable count of the L.casei HM-09 in the compound lactobacillus probiotics is more than or equal to 2.5x108cfu/g, and viable count of the L.plantarum HM-10 is more than or equal to 2.5x108cfu/g. The lactobacillus casei and the lactobacillus plantarum are preserved in China General Microbiological Culture Collection Center of China Committee for Culture Collection of Microorganisms, in Datun road, Beijing, on October 29th, 2012, and the preservation numbers of the lactobacillus casei HM-09 (L.casei HM-09) and the lactobacillus plantarum HM-10(L.plantarum HM-10) are CGMCC No.6736 and CGMCC No.6741 respectively.
Description
Technical field
The present invention relates to prevent and improve the compound micro-ecological preparation of garget, especially a kind of compound micro-ecological preparation that can effectively reduce effective prevention of somatic number, the minimizing recessive mastitis incidence of disease and improve garget.
Background technology
Mammitis is the incidence of disease the fastest the highest, popular, one of maximum disease that causes damage in the world milk cattle cultivating industry, at milk cow incidence of disease lactation period up to more than 78%.Recessiveness still is the decline that clinic mastitis all can cause the output of milk and milk quality, already causes very big harm for milk cattle cultivating, comprising: directly cause the minimizing of the output of milk; The milk processing characteristics is bad, makes Quality of Dairy Products descend; Milk modification; Medicament residue can not eat people; Some milk cows can't be cured and are eliminated.And the loss that recessive mastitis causes will be higher than clinic mastitis, and this loss is that people can not observe directly.
Utilizing the antibiotic therapy mammitis is modal methods for the treatment of, but antibiotic extensive application brings many negative effects, as appearance of antibiotic residue, antibody-resistant bacterium etc.And the anti-therapy of novel biological control method, herbal treatment, probiotics is risen gradually.
Utilize the probiotics prevention and improve garget, because it has and strengthens animal body immunity, keeps the intestinal microecology balance, produces multiple function such as useful metabolite, and have no side effect, advantage such as not residual, free from environmental pollution, therefore, can be by the development probiotics, strengthen immunity and the premunition of milk cow with probiotics, reach the purpose of control cow subclinical mastitis, have big potentiality and vast potential for future development.
The present invention is directed to above-mentioned problem, develop a kind of novel Lactobacillus casei HM-09 (L.casei HM-09) and Lactobacillus plantarum HM-10(L.plantarum HM-10) compound micro-ecological preparation, can be good at definite value behind the feeding cow in gastrointestinal tract mucous, suppressing pathogen grows, improve the intestinal beneficial bacterium group, strengthen immunity of organisms; Can effectively reduce somatic number, reduce the recessive mastitis incidence of disease.
Summary of the invention
The object of the present invention is to provide a kind of compound micro-ecological preparation that can effectively reduce the prevention of somatic number, the minimizing recessive mastitis incidence of disease and improve garget.
The objective of the invention is to be achieved through the following technical solutions:
A kind of effective prevention and improve the compound micro-ecological preparation of garget, it is characterized in that: Lactobacillus casei HM-09 in every gram compound micro-ecological preparation (L.casei HM-09) mass fraction is 10%, Lactobacillus plantarum HM-10(L.plantarum HM-10) mass fraction is 10%, all the other 80% are zeolite powder, simultaneously L.casei HM-09 number of viable 〉=2.5 * 10 in the compound micro-ecological preparation
8Cfu/g, L.plantarum HM-10 number of viable 〉=2.5 * 10
8Cfu/g;
Described Lactobacillus casei HM-09 (L.casei HM-09) and Lactobacillus plantarum HM-10(L.plantarum HM-10) be Da Tun road, Beijing in the address on October 29th, 2012, China Committee for Culture Collection of Microorganisms's common micro-organisms center preservation, Lactobacillus casei HM-09 (L.casei HM-09) culture presevation number: CGMCC No.6736; Lactobacillus plantarum HM-10(L.plantarum HM-10) culture presevation number: CGMCC No.6741.
L.casei HM-09 separates in healthy Roll road, can be good at definite value in gastrointestinal tract mucous, suppresses pathogen and grows, and improves the intestinal beneficial bacterium group, strengthens immunity of organisms; L.plantarum HM-10 separates in the feed that ferments, and effective degradative fungi toxin has good acid resistance, energy for growth is strong, acid production speed is fast.
Described probiotics is in prevention and improve application in the mastitis for milk cows, and concrete grammar is as follows:
The scheme of feeding: 100g probiotics powder and feed are mixed, and disposable feeding suffers from the ox of mammitis then, feeds continuously 21 days.
The probiotics 100 of feeding restrains, every ox every day each 1 time sooner or later, fed continuously 10 days, utilize the somatic number in the DHI detection method test cow's milk then, the result shows: compound micro-ecological preparation can effectively improve mastitis for milk cows in the claim 1 of feeding, and can significantly reduce the body cell of suffering from the mammitis cow's milk.
Probiotics provided by the invention has following good effect:
After compound micro-ecological preparation of the present invention and feed mix, feed and suffer from the milk cow of mammitis, 100g feeds every day, fed continuously 21 days, the mammitis ox is had excellent prevention and improvement effect, the mammitis incidence of disease can be reduced by 84.7%, the incidence of disease obviously descends, and improves the milk cow health level.
Feed and contain the feed of 100 gram compound micro-ecological preparations, fed continuously 10 days every ox every day each 1 time sooner or later, can effectively reduce somatic number, reduced rate is 78.3%, improves milk quality.
Description of drawings
Accompanying drawing is that feed mammitis before and after the probiotics is improved the schematic diagram of situation.
The specific embodiment
The invention will be further described below in conjunction with embodiment; Following embodiment is illustrative, does not limit protection scope of the present invention.
Experimental technique among the following embodiment if no special instructions, is conventional method.
A kind of effective prevention and improve the compound micro-ecological preparation of garget, Lactobacillus casei HM-09 in every gram compound micro-ecological preparation (L.casei HM-09) mass fraction is 10%, Lactobacillus plantarum HM-10(L.plantarum HM-10) mass fraction is 10%, all the other 80% are zeolite powder, simultaneously L.casei HM-09 number of viable 〉=2.5 * 10 in the compound micro-ecological preparation
8Cfu/g, L.plantarum HM-10 number of viable 〉=2.5 * 10
8Cfu/g;
Described Lactobacillus casei HM-09 (L.casei HM-09) and Lactobacillus plantarum HM-10(L.plantarum HM-10) be Da Tun road, Beijing in the address on October 29th, 2012, China Committee for Culture Collection of Microorganisms's common micro-organisms center preservation, Lactobacillus casei HM-09 (L.casei HM-09) culture presevation number: CGMCC No.6736; Lactobacillus plantarum HM-10(L.plantarum HM-10) culture presevation number: CGMCC No.6741.
L.casei HM-09 separates in healthy Roll road, can be good at definite value in gastrointestinal tract mucous, suppresses pathogen and grows, and improves the intestinal beneficial bacterium group, strengthens immunity of organisms; L.plantarum HM-10 separates in the feed that ferments, and effective degradative fungi toxin has good acid resistance, energy for growth is strong, acid production speed is fast.
Experiment 1:L.casei HM-09 and the improvement effect of L.plantarum HM-10 compound micro-ecological preparation to the different breasts of mammitis district.
Utilize compound micro-ecological preparation respectively in Heilungkiang, the different pastures in Tianjin, Shandong, Henan and the Inner Mongol test, 304 breast districts to 76 mammitis oxen investigate, feed before the probiotics, each breast district is carried out the CTM method to be detected, the weight of the different breasts of diagnosis district mammitis degree, feed then and contain the feed of 100 gram probioticses, every ox every day 1 time, fed continuously 21 days, different breasts district is carried out the CTM method again to be detected, detection effect before and after the probiotics of feeding is compared, observe its improvement situation.Different breasts district, the probiotics front and back mammitis situations of change of feeding see Table 1.
The table 1 different breasts district mammitis situations of change (total breast district 304) before and after the probiotics of feeding
Annotate: " ★ " more many representatives mammitis more serious, efficient=(experiment foremilk district number-test rear milk area number)/test foremilk district number) * 100%
By experimental result as can be known, this probiotics feed continuously after 21 days, mammitis be improved significantly, and the more serious improved effect in breast district of mammitis is more obvious, descended 64.4%, 53.3%, 48.5% from high to low respectively by the mammitis order of severity, hence one can see that, and this compound micro-ecological preparation has good preventing and improves the effect of garget.
Experiment 2:L.casei HM-09 and L.plantarum HM-10 compound micro-ecological preparation are to the somatic improvement effect in mammitis Ruzhong.
Utilize compound micro-ecological preparation to different pastures, the Inner Mongol, 10 oxen that suffer from mammitis carry out the body cell test, feed before the probiotics, utilize the somatic number in the DHI detection method test cow's milk, feed then and contain the feed of 100 gram probioticses, every ox respectively feeds 1 time every day sooner or later, feeds continuously 10 days, utilize the DHI detection method to test somatic number in the cow's milk again then, the somatic number before and after the compound micro-ecological preparation of feeding is compared.Result of the test sees Table 2.
Table 2 (the unit: ten thousand/mL) of somatic number situation of change in the cow's milk of feeding before and after the compound micro-ecological preparation
By table 2 data analysis as can be known, obviously descending appears in 10 somatic numbers of testing 9 oxen in the ox, 1 ox is only arranged to no effect.If be clinic mastitis by somatic number greater than 500,000/mL, result of the test explanation so: wherein 4 oxen are cured, and 5 ox effects are obvious.The average body cell number is 784.4 ten thousand/mL before 10 ox tests, and test back average body cell number is 170.5 ten thousand/mL, has descended 78.3%, shows that the compound micro-ecological preparation of feeding can effectively improve mastitis for milk cows, reduces the body cell in the cow's milk.
Experiment 3:L.casei HM-09 and L.plantarum HM-10 compound micro-ecological preparation are to the improvement effect of mastitis for milk cows breast.
Utilize compound micro-ecological preparation that 85 oxen in the different pastures in Heilungkiang, Tianjin, Shandong, Henan and the Inner Mongol are tested, consider from improving the mammitis whole structure: namely at least one breast is distinguished from improve breast, perhaps serious breast is distinguished to have clear improvement and is set out, and investigates probiotics to the improvement situation of mammitis; Process of the test is fed to every ox and is contained the feed of 100 gram compound micro-ecological preparations, every ox every day 1 time, fed continuously 21 days, improvement situation by mammitis before and after the CTM method determination test, the result shows that 72 cow head mammitises obviously improve, efficiently reach 84.7%, result of the test See Figure 1 illustrates that this compound micro-ecological preparation can effectively prevent and improve mastitis for milk cows.
Claims (3)
1. compound micro-ecological preparation that effectively prevents and improve garget, it is characterized in that: Lactobacillus casei HM-09 in every gram compound micro-ecological preparation (L.casei HM-09) mass fraction is 10%, Lactobacillus plantarum HM-10(L.plantarum HM-10) mass fraction is 10%, all the other 80% are zeolite powder, simultaneously L.casei HM-09 number of viable 〉=2.5 * 10 in the compound micro-ecological preparation
8Cfu/g, L.plantarum HM-10 number of viable 〉=2.5 * 10
8Cfu/g;
Described L.casei HM-09 separates in healthy Roll road, has excellent acidproof, bile tolerance and enteron aisle definite value ability, can promote the intestinal mucosa immunity, suppresses enteropahtogenic microganism, regulates gut flora; Described L.plantarum HM-10 separates in the feed that ferments, and effectively the degradative fungi toxin suppresses enteropahtogenic microganism; Above-mentioned two strain bacterial strains have been preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center, Lactobacillus casei HM-09 (L.casei HM-09) culture presevation number: CGMCC No.6736; Lactobacillus plantarum HM-10(L.plantarum HM-10) culture presevation number: CGMCC No.6741.
2. effectively prevent and improve the using method of the compound micro-ecological preparation of garget, it is characterized in that: probiotics 100 grams of feeding, every day 1 time, after feeding continuously 21 days, detect mammitis by the CTM method and improve situation, test shows: to mammitis prevention and improve and have remarkable result, and the more serious improved effect in breast district of mammitis is more obvious.
3. effectively prevent and improve the using method of the compound micro-ecological preparation of garget, it is characterized in that: probiotics 100 grams of feeding, every ox every day each 1 time sooner or later, fed continuously 10 days, utilize the somatic number in the DHI detection method test cow's milk then, the result shows: compound micro-ecological preparation can effectively improve mastitis for milk cows in the claim 1 of feeding, and can significantly reduce the body cell of suffering from the mammitis cow's milk.
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| CN105748534A (en) * | 2016-04-29 | 2016-07-13 | 内蒙古和美科盛生物技术有限公司 | Composite lactobacillus nipple cleaning solution for improving dairy cow nipple micro-ecosystem |
| CN109306327A (en) * | 2017-07-27 | 2019-02-05 | 内蒙古蒙正牧康实业有限公司 | The fresh storage fermenting agent of niblet and fresh storage zymotechnique |
| CN117070413A (en) * | 2023-08-21 | 2023-11-17 | 广东海洋大学 | Lactobacillus paracasei BY5 and application thereof |
| CN117070413B (en) * | 2023-08-21 | 2024-05-31 | 广东海洋大学 | Lactobacillus paracasei BY5 and application thereof |
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| CN103202391B (en) | 2014-12-31 |
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