CN101185480A - Microbial feed additive capable of accelerating lactation and improving milk product quality and preparation method - Google Patents
Microbial feed additive capable of accelerating lactation and improving milk product quality and preparation method Download PDFInfo
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- CN101185480A CN101185480A CNA2005100290575A CN200510029057A CN101185480A CN 101185480 A CN101185480 A CN 101185480A CN A2005100290575 A CNA2005100290575 A CN A2005100290575A CN 200510029057 A CN200510029057 A CN 200510029057A CN 101185480 A CN101185480 A CN 101185480A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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Abstract
The invention relates to a microbial feed additive for promoting lactation and improving the quality of dairy products and a production method thereof, and is characterized in that raw materials of lactobacillus plantarum, streptococcus lacticus, lactobacillus casei, saccharomyces cereviseae, nutritive medium, sterile water, etc., are first treated with anaerobic fermentation; fermentation liquor are then mixed after centrifugation and blended with nutrition carriers evenly to be placed in a closed fermentation chamber for anaerobic fermentation and finally crushed into power after dried so as to obtain the finished products. The invention comprises a great deal of effective microorganisms, antioxidants of coenzyme, SOD and vitamins, etc., and physiological metabolins of amino acid and active peptides, etc. which can have effects of nutrifying and repairing cell and improving cell metabolism after entering the body of a cow so as to form sound ecological environment in the cow body, promote transformation and absorption of nutrient matters and immunity of the organism and even prevent garget of the cow and improve the ability of anti-heat stress and the quality of dairy products while improving the milk yield of cows.
Description
Technical field
The present invention relates to a kind of additive for microbe feedstuff and preparation method that promotes lactation and improve the milk products quality, after this product enters in the milk cow body, can impel the conversion of nutriment to absorb, improve immunity of organisms, when improving milk production of cow, also can prevent mastitis for milk cows, improve anti-heat stress ability and milk products quality, belong to the agro-ecology product technique field.
Background technology
At present, the patent of having applied for, application number is CN02136360, notification number is CN1471847, be entitled as Glyptal composition and its production and application, it is characterized in that: microorganism fungus kind is made fermented and cultured after Liquid Culture, fermentate carries out the nuclease hydrolysis after with the alkali extracting and makes nucleotides; To use protease hydrolytic after the fragmentation of animal internal organs, the homogenate, heat treatment, supernatant after filtration, dialysis back freeze concentration, make the cytoactive polypeptide; The two mixes by a certain percentage.This Glyptal composition only is the simple mixing that two kinds of commonsense methods prepare product, does not have outstanding novel part on technology.
Summary of the invention
The purpose of this invention is to provide a kind of additive for microbe feedstuff and preparation method that promotes lactation and improve the milk products quality, it is the product that obtains by after the beneficial bacterium fermentation of certain optimisation, and selected beneficial bacterium has more OH radical scavenging activity; Culture medium is as nutriment, and after part was utilized by thalline, the enzyme that produces by bacterial metabolism decomposed the organic substance in the culture medium, and combination under given conditions, can form the anti-oxidant product of various active.After in entering the milk cow body; excessive free radical in its contained Green Tea Extract material energy antagonist; avoid the oxidative damage organized; also can play the effect of nutrition and repair cell, promotion cellular metabolism, form the optimum ecological environment in the milk cow body, impel the conversion of nutriment to absorb; improve immunity of organisms; when improving milk production of cow, also can prevent mastitis for milk cows, improve anti-heat stress ability and milk products quality etc.
For realizing above purpose, technical scheme of the present invention provides a kind of microbiological feed of lactation and raising milk products quality that promotes and adds, it is characterized in that, prepare by following weight percentages before the anaerobic fermentation: bacterial classification stoste 3-18%, culture medium 10-30%, carrier 52-87%.
Described bacterial classification stoste is at least two kinds of compositions in Lactobacillus plantarum, streptococcus lactis, Lactobacillus casei, the saccharomyces cerevisiae, is prepared by following weight percentages: Lactobacillus plantarum 20-40%, streptococcus lactis 18-38%, Lactobacillus casei 0-20%, saccharomyces cerevisiae 25-45%.
Described culture medium is at least two kinds of mixtures in soya-bean cake powder, starch, dried silkworm chrysalis meal, dusty yeast, corn steep liquor, corn flour, molasses, potassium dihydrogen phosphate, sodium hydrogen phosphate, magnesium sulfate, the sterilized water, and its weight percent proportioning is: soya-bean cake powder 1.5-30%, corn flour 2-40%, starch 0-15%, dusty yeast 0-10%, dried silkworm chrysalis meal 0-5%, corn steep liquor 0-5%, molasses 0-15%, sodium hydrogen phosphate 0-2%, potassium dihydrogen phosphate 0-2%, magnesium sulfate 0-3%, water 10-95%.
Described nutrition carrier is the mixture of calcium carbonate, bran powder, albumen powder, and its percentage by weight proportioning is: calcium carbonate 10-30%, bran powder 60-85%, albumen powder 3-10%.
The preparation method of the additive for microbe feedstuff of described promotion lactation and raising milk products quality is characterized in that the bacterial classification of employing all comes by screening and optimizing; Adopt anaerobic fermentation and low temperature cultured method simultaneously, it comprises the following steps:
The first step. respectively Lactobacillus plantarum, streptococcus lactis, Lactobacillus casei, saccharomyces cerevisiae are utilized the method for auxotrophy or uv induction to carry out preferably;
Second step. in the meat soup culture medium of respectively above-mentioned bacterial classification being transferred, after cultivating 22-26 hour under the 35-40 ℃ of temperature, measure its activity and tire, select the bacterial classification that growth is fast, the quantity in broth bouillon reaches more than 4,000,000,000/ml to preserve;
The 3rd goes on foot. and bacterial classification is transferred be equipped with in the nutrient agar eggplant bottle, under 35-40 ℃ of temperature, cultivated 45-50 hour, put into 2-6 ℃ refrigerator then and preserve;
The 4th step. one of Lactobacillus plantarum, streptococcus lactis, Lactobacillus casei, saccharomyces cerevisiae or their mixture are inoculated in respectively through in the culture medium of steam sterilizing, putting into mixer stirs, speed of agitator is 120r/min-180r/min, cultivation temperature is 35-40 ℃, throughput 1: 0.5, fermentation time is 22-26 hour, obtains composite bacteria agent capable stoste;
The 5th step. composite bacteria agent capable stoste is added in culture medium and the nutrition carrier according to special ratios, mix, insert airtight container, put into the ferment at constant temperature room, cultivation temperature is 30-35 ℃, and fermentation time is 12-18 days;
The 6th step. the tunning that obtains is put into low temperature circulation incubator, be 20-30 ℃ in temperature and continue to cultivate 65-75 hour down, make moisture less than 11%;
The 7th step. will cultivate the product after finishing, carry out crushing screening after, promptly obtain the rich sharp newborn Kang Chengpin of poultry of pulverous microorganism.
Free radical is the free molecule that has unpaired electron, atom or the ion that exists.Their chemical property is very active, can react with the many materials in the organism.The free radical growth and decline in the metabolic process in vivo is a kind of normal Physiology and biochemistry phenomenons, is in dynamic balance state.
Free radical kind in the body is more, but the oxygen source person with toxicity mainly be O2-1, O2 ,-OH and H2O, wherein with O2-for very, studies show that can produce excessive O2-when following three kinds of situations: 1. organism makes the autoxidation of the cellular components such as unrighted acid in the body lipid under irradiations such as visible rays, ultraviolet ray or radioactive ray; 2. the SOD of body self produces not enoughly, maybe can only satisfy eubolism and can not in time remove integral body or the local O2-that produces under emergent or specified conditions; 3. when leucocyte is engulfed pathogen in a large number.Oxygen radical (Oxygen Free Radical, OFR) can be by causing peroxidatic reaction of lipid, large biological molecule material and other mechanism such as injury protein matter and nucleic acid cause the histocyte damage, influence the relatively stable of organismic internal environment, thereby promote the generation and the development of numerous disease.
The milk cow of intensive farm, because feeding environment changes, eqpidemic disease threatens, stress, the application of former oxidant, antibiotic and chemicals etc. in the nutrition restriction, feed, a large amount of generations of oxygen radical in the induced animal body cause the unbalance of the interior removing free radical enzyme system (SOD, CAT, GSH) that self exists of body.In post-partum period, cow whole machine body, especially reproductive organs are bearing violent stress reaction.Simultaneously, through childbirth, birth canal exists many shallow table wounds, cervix is not closed as yet, and uterine cavity is gathering a large amount of lochia, and uterine mucosa does not also have new epithelize to cover, the mucous membrane deep layer does not form the leucocyte barrier as yet, these all provide advantage to pathogenic microorganism invasion and breeding, thereby the irregular fluctuation of the MDA that takes place during this, SOD trend of rising gradually, have in fact reacted the anti-infective purification repair process in uterus of fierce variation.The cow whole machine body, especially reproduction is because the activity and the oxidability of free radical are extremely strong, it can attack any molecule that it runs into by oxidation, make macromolecular substances generation peroxidating sex change, crosslinked or fracture in the body, thereby cause the destruction of eucaryotic cell structure and function, cause the variation of body tissue infringement and organ degeneration, had a strong impact on the milk cow immunocompetence and, caused the generation of mastitis for milk cows the resistance of disease.Particularly under the heat stress condition, mammitis incidence height, the output of milk seriously descend, cause and oestrus, ovulate undesired, form infertile or conception rate obviously descends, the ox embryo is early stage dead, the incidence of disease of retention of afterbirth rises, in addition some milk cow since for a long time infertile being forced to eliminate.The milk cow of high yield is influenced big more.
The antioxidant that the sharp newborn health feed addictive of rich poultry is a kind of microbial source, its contained Green Tea Extract material can antagonist in excessive free radical, avoid the oxidative damage organized, thus the generation of prevention relevant disease, safeguard body health, improve service life and the productivity effect of milk cow.
Advantage of the present invention is:
1. effectively remove the active oxygen radical that produces in the milk cow body, the normal configuration of protection cell membrane, the normal function of assurance activity in vivo molecule;
2. promote the growth of immunity of organism organ, improve immunity of organisms and comprehensive premunition;
3. obviously improve milk yield;
4. obviously improve the dairy fat content of milk;
5. the vigor of protection mammary glandular cell significantly reduces Ruzhong body cell content, to preventing and treating mammitis remarkable effect is arranged.
The specific embodiment
Below in conjunction with embodiment the present invention is described in further detail:
Embodiment one
Promote the bacterial classification of the preparation method employing of lactation and the additive for microbe feedstuff that improves the milk products quality all to come, adopt simultaneously and detest by screening and optimizing
Aerobe fermentation and low temperature cultured method,, it comprises the following steps:
The first step. respectively Lactobacillus plantarum, streptococcus lactis, Lactobacillus casei, saccharomyces cerevisiae are utilized the method for auxotrophy or uv induction to carry out preferably;
Second step. in the meat soup culture medium of respectively above-mentioned bacterial classification being transferred, after cultivating 24 hours under 37 ℃ of temperature, measure its activity and tire, select the bacterial classification that growth is fast, the quantity in broth bouillon reaches more than 4,000,000,000/ml to preserve;
The 3rd goes on foot. and bacterial classification is transferred be equipped with in the nutrient agar eggplant bottle, under 37 ℃ of temperature, cultivated 48 hours, put into 4 ℃ refrigerator then and preserve;
The 4th step. Lactobacillus plantarum, streptococcus lactis, Lactobacillus casei, saccharomyces cerevisiae are inoculated in respectively through in the culture medium of steam sterilizing according to 20%, 25%, 15% and 40% ratio, and culture medium prescription is:
Soya-bean cake powder 2.5%, corn flour 4%, molasses 0.5%, potassium dihydrogen phosphate 0.08%, magnesium sulfate 0.1%, water 92.82% are put into mixer and are stirred, and speed of agitator is 160r/min, cultivation temperature is 37 ℃, throughput 1: 0.5, fermentation time is 24 hours, obtains composite bacteria agent capable stoste;
The 5th step. composite bacteria agent capable stoste is added in culture medium and the nutrition carrier according to special ratios, take by weighing by following weight proportion, bacterial classification stoste 10%, culture medium 21%, carrier 69%, wherein, each components in proportions is in the culture medium: soya-bean cake powder 25%, corn flour 30%, starch 10%, dusty yeast 5%, corn steep liquor 2%, molasses 10%, sodium hydrogen phosphate 1%, magnesium sulfate 2%, water 15%; The concrete ratio of nutrition carrier is: bran powder 75%, calcium carbonate 17%, albumen powder 8%, mix, and insert airtight container, put into the ferment at constant temperature room, cultivation temperature is 32 ℃, fermentation time is 15 days;
The 6th step. the tunning that obtains is put into low temperature circulation incubator, be 25 ℃ in temperature and continue to cultivate 72 hours down, make moisture less than 11%;
The 7th step. will cultivate the product after finishing, carry out crushing screening after, promptly obtain the rich sharp newborn Kang Chengpin of poultry of pulverous microorganism.
Embodiment two
The 4th step. Lactobacillus plantarum, streptococcus lactis, saccharomyces cerevisiae are inoculated in respectively through in the culture medium of steam sterilizing according to 30%, 30%, 40% ratio, culture medium prescription is: soya-bean cake powder 2.5%, corn flour 4%, molasses 0.5%, potassium dihydrogen phosphate 0.08%, magnesium sulfate 0.1%, water 92.82%, putting into mixer stirs, speed of agitator is 160r/min, cultivation temperature is 37 ℃, throughput 1: 0.5, fermentation time is 24 hours, obtains composite bacteria agent capable stoste;
The 5th step. composite bacteria agent capable stoste is added in culture medium and the nutrition carrier according to special ratios, take by weighing by following weight proportion, bacterial classification stoste 12%, culture medium 22%, carrier 66%, wherein, each components in proportions is in the culture medium: soya-bean cake powder 30%, corn flour 25%, starch 15%, dusty yeast 5%, corn steep liquor 5%, dried silkworm chrysalis meal 4%, magnesium sulfate 1%, water 14%, the concrete ratio of nutrition carrier is: bran powder 72%, calcium carbonate 20%, albumen powder 8%, mix, insert airtight container, put into the ferment at constant temperature room, cultivation temperature is 32 ℃, and fermentation time is 15 days; Other steps are identical with embodiment 1.
Claims (4)
1. an additive for microbe feedstuff that promotes lactation and improve the milk products quality is characterized in that, is prepared by following weight percentages before the anaerobic fermentation: bacterial classification stoste 3-18%, culture medium A 10-30%, carrier 52-87%,
Described bacterial classification stoste is at least two kinds of compositions in Lactobacillus plantarum, streptococcus lactis, Lactobacillus casei, the saccharomyces cerevisiae, by the configuration of the bacterial classification of following weight percentage: Lactobacillus plantarum 20-40%, streptococcus lactis 18-38%, Lactobacillus casei 0-20%, saccharomyces cerevisiae 25-45%.
2. the additive for microbe feedstuff of promotion lactation according to claim 1 and raising milk products quality, it is characterized in that, described culture medium is the soya-bean cake powder, starch, dried silkworm chrysalis meal, dusty yeast, corn steep liquor, corn flour, molasses, potassium dihydrogen phosphate, sodium hydrogen phosphate, magnesium sulfate, at least two kinds of mixtures in the sterilized water, its weight percent proportioning is: soya-bean cake powder 1.5-30%, corn flour 2-40%, starch 0-15%, dusty yeast 0-10%, dried silkworm chrysalis meal 0-5%, corn steep liquor 0-5%, molasses 0-15%, sodium hydrogen phosphate 0-2%, potassium dihydrogen phosphate 0-2%, magnesium sulfate 0-3%, water 10-95%.
3. the additive for microbe feedstuff of promotion lactation according to claim 1 and raising milk products quality, it is characterized in that, described nutrition carrier is the mixture of calcium carbonate, bran powder, albumen powder, and its weight percent proportioning is: calcium carbonate 10-30%, bran powder 60-85%, albumen powder 3-10%.
4. according to the preparation method of the described promotion lactation of claim l, it is characterized in that the bacterial classification of employing all comes by screening and optimizing with the additive for microbe feedstuff that improves the milk products quality; Adopt anaerobic fermentation and low temperature cultured method simultaneously,, it comprises the following steps:
The first step. respectively Lactobacillus plantarum, streptococcus lactis, Lactobacillus casei, saccharomyces cerevisiae are utilized the method for auxotrophy or uv induction to carry out preferably;
Second step. in the meat soup culture medium of respectively above-mentioned bacterial classification being transferred, after cultivating 22-26 hour under the 35-40 ℃ of temperature, measure its activity and tire, select the bacterial classification that growth is fast, the quantity in broth bouillon reaches more than 4,000,000,000/ml to preserve;
The 3rd goes on foot. and bacterial classification is transferred be equipped with in the nutrient agar eggplant bottle, under 35-40 ℃ of temperature, cultivated 45-50 hour, put into 2-6 ℃ refrigerator then and preserve;
The 4th step. one of Lactobacillus plantarum, streptococcus lactis, Lactobacillus casei, saccharomyces cerevisiae or their mixture are inoculated in respectively through in the culture medium of steam sterilizing, putting into mixer stirs, speed of agitator is 120r/min-180r/min, cultivation temperature is 35-40 ℃, throughput 1: 0.5, fermentation time is 22-26 hour, obtains composite bacteria agent capable stoste;
The 5th step. composite bacteria agent capable stoste is added in culture medium and the nutrition carrier according to special ratios, mix, insert airtight container, put into the ferment at constant temperature room, cultivation temperature is 30-35 ℃, and fermentation time is 12-18 days;
The 6th step. the tunning that obtains is put into low temperature circulation incubator, be 20-30 ℃ in temperature and continue to cultivate 65-75 hour down, make moisture less than 11%;
The 7th step. will cultivate the product after finishing, carry out crushing screening after, promptly obtain the rich sharp newborn Kang Chengpin of poultry of pulverous microorganism.
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| Application Number | Priority Date | Filing Date | Title |
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| CNA2005100290575A CN101185480A (en) | 2005-08-24 | 2005-08-24 | Microbial feed additive capable of accelerating lactation and improving milk product quality and preparation method |
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| CNA2005100290575A CN101185480A (en) | 2005-08-24 | 2005-08-24 | Microbial feed additive capable of accelerating lactation and improving milk product quality and preparation method |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102550857A (en) * | 2011-12-19 | 2012-07-11 | 北京科兴大地饲料有限公司 | Functional feed for preventing and treating mastitis in dairy cows and preparation method thereof |
| CN103202391A (en) * | 2013-03-22 | 2013-07-17 | 内蒙古和美科盛生物技术有限公司 | Compound lactobacillus probiotics for effectively preventing and improving bovine mastitis |
| CN105166372A (en) * | 2015-09-14 | 2015-12-23 | 安徽三兴饲料有限公司 | Feed for dairy cows at lactation later period |
| CN105748534A (en) * | 2016-04-29 | 2016-07-13 | 内蒙古和美科盛生物技术有限公司 | Composite lactobacillus nipple cleaning solution for improving dairy cow nipple micro-ecosystem |
| CN106491658A (en) * | 2016-12-29 | 2017-03-15 | 泰安大凡神农制药有限公司 | A kind of probiotic composition with promotion lactogenic function and preparation method and application |
| CN107251997A (en) * | 2017-05-27 | 2017-10-17 | 梁慰爱 | A kind of lactation period feed of milk cow and preparation method thereof |
| CN107950783A (en) * | 2017-12-12 | 2018-04-24 | 湖南德人牧业科技有限公司 | A kind of fine fodder for preventing mastitis for milk cows and preparation method thereof, equipment |
| CN109362962A (en) * | 2018-12-28 | 2019-02-22 | 宁波天赋饲料科技有限公司 | A kind of fermented feed reducing lactation middle and later periods cow heat stress |
| CN114766601A (en) * | 2022-05-12 | 2022-07-22 | 西藏自治区农牧科学院畜牧兽医研究所 | Microbial starter for improving lactation performance of plateau yaks and preparation method thereof |
-
2005
- 2005-08-24 CN CNA2005100290575A patent/CN101185480A/en active Pending
Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102550857A (en) * | 2011-12-19 | 2012-07-11 | 北京科兴大地饲料有限公司 | Functional feed for preventing and treating mastitis in dairy cows and preparation method thereof |
| CN102550857B (en) * | 2011-12-19 | 2013-08-07 | 北京科兴大地饲料有限公司 | Functional feed for preventing and treating mastitis in dairy cows and preparation method thereof |
| CN103202391A (en) * | 2013-03-22 | 2013-07-17 | 内蒙古和美科盛生物技术有限公司 | Compound lactobacillus probiotics for effectively preventing and improving bovine mastitis |
| CN103202391B (en) * | 2013-03-22 | 2014-12-31 | 内蒙古和美科盛生物技术有限公司 | Compound lactobacillus probiotics for effectively preventing and improving bovine mastitis |
| CN105166372A (en) * | 2015-09-14 | 2015-12-23 | 安徽三兴饲料有限公司 | Feed for dairy cows at lactation later period |
| CN105748534A (en) * | 2016-04-29 | 2016-07-13 | 内蒙古和美科盛生物技术有限公司 | Composite lactobacillus nipple cleaning solution for improving dairy cow nipple micro-ecosystem |
| CN105748534B (en) * | 2016-04-29 | 2018-12-18 | 内蒙古和美科盛生物技术有限公司 | A kind of compound lactobacillus nipple cleaning solution improving mammilla of milk cattle microecosystem |
| CN106491658A (en) * | 2016-12-29 | 2017-03-15 | 泰安大凡神农制药有限公司 | A kind of probiotic composition with promotion lactogenic function and preparation method and application |
| CN107251997A (en) * | 2017-05-27 | 2017-10-17 | 梁慰爱 | A kind of lactation period feed of milk cow and preparation method thereof |
| CN107950783A (en) * | 2017-12-12 | 2018-04-24 | 湖南德人牧业科技有限公司 | A kind of fine fodder for preventing mastitis for milk cows and preparation method thereof, equipment |
| CN109362962A (en) * | 2018-12-28 | 2019-02-22 | 宁波天赋饲料科技有限公司 | A kind of fermented feed reducing lactation middle and later periods cow heat stress |
| CN114766601A (en) * | 2022-05-12 | 2022-07-22 | 西藏自治区农牧科学院畜牧兽医研究所 | Microbial starter for improving lactation performance of plateau yaks and preparation method thereof |
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