CN104017751A - Probiotics prepared from bacillus coagulans and lactobacillus plantarum as well as preparation method thereof - Google Patents
Probiotics prepared from bacillus coagulans and lactobacillus plantarum as well as preparation method thereof Download PDFInfo
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Abstract
The invention discloses probiotics prepared from bacillus coagulans and lactobacillus plantarum as well as a preparation method thereof and belongs to the technical field of microorganisms of feeding stuff. The probiotics is prepared by carrying out solid-state fermentation on matrix by using bacillus coagulans, lactobacillus plantarum and lactobacillus casei, wherein the matrix includes soybean meal, bran and corn flour. The fermentation method has the following advantages that the fermentation cost is reduced, the processing is convenient, the pollution is hardly caused, equipment is simple in structure and easy to operate; and therefore the fermentation method is a probiotics solid fermentation technology which can be widely applied to the field of micro-ecology; the probiotics disclosed by the invention is capable of degrading macromolecule proteins in the soybean meal, the bran and the corn flour into oligopeptides and completely decomposing anti-nutrients, antigen protein and the like in the protein, so that the protein quality and the feeding value of fermented products such as the soybean meal are improved; and the fermented products contain viable bacteria and are capable of effectively adjusting microbial florae of animal intestines, improving production performance and increasing the utilization rate of the feeding stuff.
Description
Technical field
The invention belongs to feed microbe technical field, particularly a kind of to take dregs of beans, wheat bran, Semen Maydis powder be matrix, utilizes Bacillus coagulans, plant lactobacillus to carry out the utilisation technology that solid state fermentation is prepared probiotic agent.
Background technology
Existing probiotic composition adopts probiotic bacterium directly and liquid cow's milk and soya-bean milk fermentation more, obtains functional yogurt milk or soybean milk yoghurt, or is adopted after liquid state fermentation, directly collects probiotic bacterium thalline, is made into probiotic bacterium pulvis, tablet or capsule.When adopting liquid state fermentation, can produce a large amount of contaminated wastewater environment, aftertreatment is more difficult, and machinery equipment operation is also comparatively loaded down with trivial details.Because probiotic bacterium can be improved host's microecological balance, improve active bacteria formulation and the meta-bolites of host health level and state of health, therefore, now the research of probiotic bacterium is goed deep into detailed-oriented very much, and be widely used in each fields such as industry, agricultural, environmental protection, family.
At present, probiotic composition is applied to animal microorganism preparation, be added in animal-feed also comparatively common; For example, publication number is patent of invention < < compound lactobacillus probiotics > > who effectively prevents and improve garget of CN103202391 A, disclosing a kind of is the lactobacterium casei HM-09 of CGMCC No.6736, the plant lactobacillus HM-10 that preserving number is CGMCC No.6741 and the compound micro-ecological preparation that zeolite powder combines by mass fraction by preserving number, can effectively reduce somatic number, prevention reduces recessive garget sickness rate; Publication number is good Bacillus coagulans and the application > > thereof that patent of invention < < mono-strain of CN103243043A cultivates for improving inferior piglet, the Bacillus coagulans that a kind of preserving number is CGMCC No.6729 is disclosed, by applying this bacterial strain, can effectively improve piglet healthy state, improve the production performance of inferior piglet cultivation.Publication number is that the patent of invention < < of CN103421711A is for the lactobacterium casei of fodder additives probiotic bacterium solid fermentation, animal bifidobacteria, plant lactobacillus and subtilis > > disclose the lactobacterium casei for fodder additives probiotic bacterium solid fermentation, animal bifidobacteria, plant lactobacillus and subtilis, described subtilis HM-66, lactobacterium casei F-08, plant lactobacillus HM-20 and animal bifidobacteria V9 are probiotic bacterium, be preserved in common micro-organisms DSMZ of China Committee for Culture Collection of Microorganisms, preserving number is respectively CGMCCNo.6733, CGMCCNo.6735, CGMCCNo.6744 and CGMCCNo.5470, by the application of this several bacterial strains, can effectively improve the organoleptic features such as sour fragrance, color and luster and mouthfeel of feed.
Summary of the invention
The object of the present invention is to provide and a kind ofly utilized the bacterium such as Bacillus coagulans (Bacillus coagulans) HM-09 and plant lactobacillus (Lactobacillus plantarum) HM-10 to dregs of beans, wheat bran and Semen Maydis powder matrix are carried out the method for segmentation solid state fermentation, the probiotic agent product that utilizes this method to ferment has special sour fragrance, and the viable count containing is high, the degradation rate of dregs of beans protein is also high, can also regulate animal intestinal microorganism species, promote intestinal absorption, enhancing body immunizing power, improve production performance, improve the utilization ratio of feed, effectively suppress rotting of cultivation later stage body ight soil and residual bait, improve environmental sanitation, reduce the generation of ammonia nitrogen and nitrite, reduce whole aquaculture cost.
The present invention is achieved through the following technical solutions:
A probiotic agent that utilizes Bacillus coagulans, plant lactobacillus to prepare, its preparation method comprises the steps:
The preparation of seed liquor: by Bacillus coagulans HM-09 30-38 ℃ in thermostat container, 170 turn shaking table cultivates 20-24h; The 30-38 ℃ of cultivation 20-24h in thermostat container by plant lactobacillus HM-10 and lactobacterium casei HM-09;
The seed culture medium of described Bacillus coagulans HM-09, plant lactobacillus HM-10 and lactobacterium casei HM-09 is: peptone 2-5%, and glucose 2-5%, adds water to 100%, pH7.0-7.2;
Fermentation culture conditions: Bacillus coagulans HM-09 seed liquor is added to and mixed secondary fermentation 0-16h in solid medium, inoculate plant lactobacillus HM-10 seed liquor and lactobacterium casei HM-09 seed liquor and continue fermentation 12-36h, amount to fermentation time 12-52h, in fermenting process, controlled fermentation temperature is 30-38 ℃, humidity is 60-80%, and its free ammonical nitrogen content of fermentation ends is more than 480umol/g;
Described solid medium is: 25-30% dregs of beans, 25-30% wheat bran, 2-5% Semen Maydis powder, moisture content 40-50%, initial pH6.8-7.2.
The addition of described Bacillus coagulans HM-09 seed liquor is the 3-6% of solid medium gross weight, the addition of plant lactobacillus HM-10 seed liquor is the 2-4% of solid medium gross weight, and the addition of lactobacterium casei HM-09 seed liquor is the 4-6% of solid medium gross weight.
Bacillus coagulans HM-09 viable count (1.45-2.98) * 10 in probiotic agent
9cFU/g, plant lactobacillus HM-10 viable count (1.55-2.65) * 10
9cFU/g, lactobacterium casei HM-09 viable count (3.31-9.98) * 10
9cFU/g.
The addition of product of the present invention in feed is 25-60g/kg.
Beneficial effect
The present invention utilizes Bacillus coagulans HM-09 and plant lactobacillus HM-10 etc., for fermenting agent, dregs of beans, wheat bran and Semen Maydis powder are carried out to mixed solid fermentation, reduced fermentative production cost, it is convenient to process, pollute little, and device structure is simple, easy to operate, it is a kind of probiotic bacterium solid-fermented technique that is widely used in micro-ecosphere; Adopt Bacillus coagulans HM-09 to carry out prior fermentation, can produce the tart flavour small-molecule substances such as a large amount of lactic acid and acetic acid, these small-molecule substances are given the distinctive sour fragrance of product, color and luster and mouthfeel etc., have improved the palatability of product; The present invention has added plant lactobacillus HM-10 and lactobacterium casei HM-09 carries out mixed fermentation at solid state fermentation after for some time, can efficiently utilize lactobacterium casei HM-09 bacterium to regulate intestinal flora balance, promote the function that body is digested and assimilated, can also utilize lactobacterium casei to promote fissional effect simultaneously, produce antibody mediated immunity, enhancing body immunity; Described plant lactobacillus is the distinctive lactobacillin of energy output in reproductive process, therefore utilize this characteristic, in the cultivation later stage, can well suppress rotting of bottom ight soil and residual bait, reduce the generation of ammonia nitrogen and nitrite, a large amount of consumptions that reduce chemical industry degradation element, reduce aquaculture cost; In the probiotics preparation that utilizes the method for segmentation solid state fermentation to produce, contain a large amount of viable bacterias, therefore, the characteristic of collaborative each bacterial classification, can effectively regulate animal intestinal microorganism species, promote intestinal absorption, enhancing body immunizing power, improve weanling pig diarrhoea with dislocation of bacillary groups, improve the utilization ratio of feed, reduce whole aquaculture cost.
The diarrhea rate that product of the present invention can effectively reduce sucking pig while being used for feeding of early weaned piglets is more than 30%, and the transformation efficiency of feed has improved 25-30%.
Embodiment
Below in conjunction with example, the present invention is further described, and following example is illustrative, is not determinate, can not limit protection scope of the present invention with following example.
Bacillus coagulans HM-09 provided by the present invention, plant lactobacillus HM-10 and lactobacterium casei (Lactobacillus casei) HM-09 is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center on October 29th, 2012, and deposit number is respectively CGMCC No.6729, CGMCC No.6741 and CGMCC No.6736; Preservation address is: Datun Road, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica, postcode: 100101;
Embodiment 1
A probiotic agent that utilizes Bacillus coagulans, plant lactobacillus to prepare, its preparation method comprises the steps:
The preparation of seed liquor: Bacillus coagulans HM-09, plant lactobacillus HM-10 and lactobacterium casei HM-09 are inoculated in the substratum that liquid seeds is housed from the glycerine pipe of cold storage, the seed culture condition of described Bacillus coagulans HM-09: 37 ℃, 170 turn shaking table cultivates 24h; Plant lactobacillus HM-10 and lactobacterium casei HM-09 be 37 ℃ of cultivation 24h in thermostat container;
Wherein seed culture medium is: peptone 2%, glucose 2%, adds water to 100%, pH7.0-7.2.
Solid state fermentation
Fermentation culture conditions: Bacillus coagulans HM-09 seed liquor is added to and mixed secondary fermentation 12h in solid medium, inoculate plant lactobacillus HM-10 seed liquor and lactobacterium casei HM-09 seed liquor and continue fermentation 24h, amount to fermentation time 36h, in fermenting process, controlled fermentation temperature is 37 ℃, humidity is 70%, and its free ammonical nitrogen content of fermentation ends is 489umol/g;
Described solid medium is: 29% dregs of beans, 29% wheat bran, 2% Semen Maydis powder, moisture content 40%, initial pH7.0,115 ℃ of autoclaving 25min.
The addition of described Bacillus coagulans HM-09 seed liquor is 5% of solid medium gross weight, the addition of plant lactobacillus HM-10 seed liquor is 3% of solid medium gross weight, and the addition of lactobacterium casei HM-09 seed liquor is 5% of solid medium gross weight.
Bacillus coagulans HM-09 viable count 2.98 * 10 in probiotic agent
9cFU/g, plant lactobacillus HM-10 viable count 2.07 * 10
9cFU/g, the viable count 5.98 * 10 of lactobacterium casei HM-09
9cFU/g.
The addition of product of the present invention in feed is 45g/kg.
Embodiment 2:
A probiotic agent that utilizes Bacillus coagulans, plant lactobacillus to prepare, its preparation method comprises the steps:
The preparation of seed liquor: by Bacillus coagulans HM-09 in thermostat container 37 ℃, 170 turn shaking table cultivates 24h; 37 ℃ of cultivation 24h in thermostat container by plant lactobacillus HM-10 and lactobacterium casei HM-09.
Wherein seed culture medium is: peptone 2%, and glucose 2%, adds water to 100%, pH7.0-7.2;
Fermentation culture conditions: Bacillus coagulans HM-09 seed liquor is added to and mixed secondary fermentation 16h in solid medium, inoculate plant lactobacillus HM-10 and lactobacterium casei HM-09 seed liquor and continue fermentation 15h, amount to fermentation time 31h, in fermenting process, controlled fermentation temperature is 37 ℃, humidity is 80%, and its free ammonical nitrogen content of fermentation ends is at 486umol/g;
Solid medium is: 29% dregs of beans, 29% wheat bran, 2% Semen Maydis powder, moisture content 40%, initial pH7.0,115 ℃ of autoclaving 25min.
The addition of Bacillus coagulans HM-09 seed liquor is 3% of solid medium gross weight, the addition of plant lactobacillus HM-10 seed liquor is 2% of solid medium gross weight, and the addition of lactobacterium casei HM-09 seed liquor is 6% of solid medium gross weight.
Bacillus coagulans HM-09 viable count 1.45 * 10 in probiotic agent
9cFU/g, plant lactobacillus HM-10 viable count 1.55 * 10
9cFU/g, lactobacterium casei HM-09 viable count 9.98 * 10
9cFU/g.
The addition of product of the present invention in feed is 30g/kg.
Embodiment 3
A probiotic agent that utilizes Bacillus coagulans, plant lactobacillus to prepare, its preparation method comprises the steps:
The preparation of seed liquor: by Bacillus coagulans HM-09 in thermostat container 37 ℃, 170 turn shaking table cultivates 24h; 37 ℃ of cultivation 24h in thermostat container by plant lactobacillus HM-10 and lactobacterium casei HM-09.
The seed culture medium of Bacillus coagulans HM-09, plant lactobacillus HM-10 and lactobacterium casei HM-09 is: peptone 2%, and glucose 2%, adds water to 100%, pH7.0-7.2;
Fermentation culture conditions: Bacillus coagulans HM-09 seed liquor is added to and mixed secondary fermentation 6h in solid medium, inoculate lactobacterium casei HM-09 and plant lactobacillus HM-10 seed liquor and continue fermentation 36h, amount to fermentation time 42h, in fermenting process, controlled fermentation temperature is 37 ℃, humidity is 60%, and its free ammonical nitrogen content of fermentation ends is at 460umol/g;
Solid medium is: 29% dregs of beans, 29% wheat bran, 2% Semen Maydis powder, moisture content 40%, initial pH7.0,115 ℃ of autoclaving 25min.
The addition of Bacillus coagulans HM-09 seed liquor is 6% of solid medium gross weight, the seed liquor addition of plant lactobacillus HM-10 is 4% of solid medium gross weight, and the addition of lactobacterium casei HM-09 seed liquor is 4% of solid medium gross weight.
Bacillus coagulans HM-09 viable count 3.18 * 10 in probiotic agent
9cFU/g, plant lactobacillus HM-10 viable count 2.65 * 10
9cFU/g, lactobacterium casei HM-09 viable count 3.31 * 10
9cFU/g.
The addition of product of the present invention in feed is 60g/kg.
Result of use experiment
The result of use test of the probiotic agent of the application embodiment of the present invention 1 in weanling pig:
It is 120 of the healthy sucking pigs of (10.5 ± 0.45) kg that experimental animal is selected plant's mean body weight, and statistical analysis body weight difference is not remarkable.Adopt single-factor randomized design, by 120 healthy sucking pigs, by male and female half and half, be divided at random 2 groups (control group and test group), every group of 6 repetitions, each repeats 10 pigs.Test group is added the product 45g/kg of the present invention that embodiment 1 makes, and control group does not add product of the present invention.Feed continuously 20 days, compare with control group, use invention fodder additives can obtain following effect:
| Project | Test group | Control group |
| Starting weight (kg) | 10.45 | 10.65 |
| End heavy (kg) | 20.57 | 19.59 |
| Daily ingestion amount (g) | 720.1 | 705.2 |
| Day weight gain (g) | 506 | 447 |
| Feedstuff-meat ratio | 1.42 | 1.58 |
| Diarrhea rate (head) | 6 | 24 |
Test-results shows: sucking pig is fed after the feed that contains probiotic agent of the present invention, and daily ingestion amount increases, and day weight gain has improved, and has also reduced the feedstuff-meat ratio of sucking pig simultaneously, and diarrhea rate has also significantly reduced.
Claims (8)
1. a probiotic agent that utilizes Bacillus coagulans, plant lactobacillus to prepare, by following methods, prepared:
The preparation of seed liquor: by Bacillus coagulans HM-09 30-38 ℃ in thermostat container, 170 turn shaking table cultivates 20-24h; The 30-38 ℃ of cultivation 20-24h in thermostat container by plant lactobacillus HM-10 and lactobacterium casei HM-09;
The seed culture medium of described Bacillus coagulans HM-09, plant lactobacillus HM-10 and lactobacterium casei HM-09 is: peptone 2-5%, and glucose 2-5%, adds water to 100%, pH7.0-7.2;
Fermentation culture conditions: Bacillus coagulans HM-09 seed liquor is added to and mixed secondary fermentation 0-16h in solid medium, inoculate plant lactobacillus HM-10 seed liquor and lactobacterium casei HM-09 seed liquor and continue fermentation 12-36h, amount to fermentation time 12-52h, in fermenting process, controlled fermentation temperature is 30-38 ℃, humidity is 60-80%, and its free ammonical nitrogen content of fermentation ends is more than 480umol/g;
Described Bacillus coagulans HM-09 deposit number is CGMCC No.6729, and plant lactobacillus HM-10 deposit number is CGMCC No.6741, and lactobacterium casei HM-09 deposit number is CGMCC No.6736.
2. the probiotic agent that utilizes Bacillus coagulans, plant lactobacillus to prepare according to claim 1, is characterized in that, described solid medium is: 25-30% dregs of beans, 25-30% wheat bran, 2-5% Semen Maydis powder, moisture content 40-50%, initial pH6.8-7.2.
3. the probiotic agent that utilizes Bacillus coagulans, plant lactobacillus to prepare according to claim 1, it is characterized in that, the addition of described Bacillus coagulans HM-09 seed liquor is the 3-6% of solid medium gross weight, the addition of plant lactobacillus HM-10 seed liquor is the 2-4% of solid medium gross weight, and the addition of lactobacterium casei HM-09 seed liquor is the 4-6% of solid medium gross weight.
4. the probiotic agent that utilizes Bacillus coagulans, preparation according to claim 3, it is characterized in that, the addition of described Bacillus coagulans HM-09 seed liquor is 5% of solid medium gross weight, the addition of plant lactobacillus HM-10 seed liquor is 3% of solid medium gross weight, and the addition of lactobacterium casei HM-09 seed liquor is 5% of solid medium gross weight.
5. utilize Bacillus coagulans, plant lactobacillus to prepare a method for probiotic agent, comprise the steps:
The preparation of seed liquor: by Bacillus coagulans HM-09 30-38 ℃ in thermostat container, 170 turn shaking table cultivates 20-24h; The 30-38 ℃ of cultivation 20-24h in thermostat container by plant lactobacillus HM-10 and lactobacterium casei HM-09;
The seed culture medium of described Bacillus coagulans HM-09, plant lactobacillus HM-10 and lactobacterium casei HM-09 is: peptone 2-5%, and glucose 2-5%, adds water to 100%, pH7.0-7.2;
Fermentation culture conditions: Bacillus coagulans HM-09 seed liquor is added to and mixed secondary fermentation 0-16h in solid medium, inoculate plant lactobacillus HM-10 seed liquor and lactobacterium casei HM-09 seed liquor and continue fermentation 12-36h, amount to fermentation time 12-56h, in fermenting process, controlled fermentation temperature is 30-38 ℃, humidity is 60-80%, and its free ammonical nitrogen content of fermentation ends is more than 480umol/g;
Described solid medium is: 25-30% dregs of beans, 25-30% wheat bran, 2-5% Semen Maydis powder, moisture content 40-50%, initial pH6.8-7.2;
Described Bacillus coagulans HM-09 deposit number is CGMCC No.6729, and plant lactobacillus HM-10 deposit number is CGMCC No.6741, and lactobacterium casei HM-09 deposit number is CGMCC No.6736.
6. the method for utilizing Bacillus coagulans, plant lactobacillus to prepare probiotic agent according to claim 5, it is characterized in that, the addition of described Bacillus coagulans HM-09 seed liquor is the 3-6% of solid medium gross weight, the addition of plant lactobacillus HM-10 seed liquor is the 2-4% of solid medium gross weight, and the addition of lactobacterium casei HM-09 seed liquor is the 4-6% of solid medium gross weight.
7. the method for utilizing Bacillus coagulans, plant lactobacillus to prepare probiotic agent according to claim 6, it is characterized in that, the addition of described Bacillus coagulans HM-09 seed liquor is 5% of solid medium gross weight, the addition of plant lactobacillus HM-10 seed liquor is 3% of solid medium gross weight, and the addition of lactobacterium casei HM-09 seed liquor is 5% of solid medium gross weight.
8. the method for utilizing Bacillus coagulans, plant lactobacillus to prepare probiotic agent according to claim 5, is characterized in that, comprises the steps:
The preparation of seed liquor: Bacillus coagulans HM-09, plant lactobacillus HM-10 and lactobacterium casei HM-09 are inoculated in the substratum that liquid seeds is housed from the glycerine pipe of cold storage, the seed culture condition of described Bacillus coagulans HM-09: 37 ℃, 170 turn shaking table cultivates 24h; Plant lactobacillus HM-10 and lactobacterium casei HM-09 be 37 ℃ of cultivation 24h in thermostat container;
Wherein seed culture medium is: peptone 2%, and glucose 2%, adds water to 100%, pH7.0-7.2;
Solid state fermentation
Fermentation culture conditions: Bacillus coagulans HM-09 seed liquor is added to and mixed secondary fermentation 12h in solid medium, inoculate plant lactobacillus HM-10 seed liquor and lactobacterium casei HM-09 seed liquor and continue fermentation 24h, amount to fermentation time 36h, in fermenting process, controlled fermentation temperature is 37 ℃, humidity is 70%, and its free ammonical nitrogen content of fermentation ends is 489umol/g;
Described solid medium is: 29% dregs of beans, 29% wheat bran, 2% Semen Maydis powder, moisture content 40%, initial pH7.0,115 ℃ of autoclaving 25min;
The addition of described Bacillus coagulans HM-09 seed liquor is 5% of solid medium gross weight, the addition of plant lactobacillus HM-10 seed liquor is 3% of solid medium gross weight, and the addition of lactobacterium casei HM-09 seed liquor is 5% of solid medium gross weight.
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105053566A (en) * | 2015-08-18 | 2015-11-18 | 山东中瀚生物科技有限公司 | Cornus walteri seed proteinase feed additive and preparation method thereof |
| CN106551081A (en) * | 2016-11-09 | 2017-04-05 | 润盈生物工程（上海）有限公司 | A kind of mixed type additive for microbe feedstuff and its application |
| CN106819625A (en) * | 2016-12-19 | 2017-06-13 | 上海创博生态工程有限公司 | A kind of mandarin fish fermented feed and preparation method thereof |
| CN108669346A (en) * | 2018-05-04 | 2018-10-19 | 江苏宏业生态农业发展有限公司 | A kind of organic Se-rich chicken feed and its preparation process |
| CN109609410A (en) * | 2019-01-08 | 2019-04-12 | 天津市畜牧兽医研究所 | A kind of preparation method of alfalfa ensilage leavening and alfalfa ensilage |
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| CN115868581A (en) * | 2023-01-07 | 2023-03-31 | 山东省农业科学院畜牧兽医研究所 | Beef cattle fermented feed and preparation method thereof |
| CN116491577A (en) * | 2023-05-08 | 2023-07-28 | 宁夏大学 | High-activity probiotic composite microecological preparation and preparation method and application thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103202391A (en) * | 2013-03-22 | 2013-07-17 | 内蒙古和美科盛生物技术有限公司 | Compound lactobacillus probiotics for effectively preventing and improving bovine mastitis |
| CN103243043A (en) * | 2013-03-22 | 2013-08-14 | 内蒙古和美科盛生物技术有限公司 | Excellent bacillus coagulans for improving inferior piglet breeding and application of bacillus coagulans |
| CN103255081A (en) * | 2013-03-22 | 2013-08-21 | 内蒙古和美科盛生物技术有限公司 | Excellent bacillus coagulans for breeding sows and application thereof |
-
2014
- 2014-04-17 CN CN201410154866.8A patent/CN104017751B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103202391A (en) * | 2013-03-22 | 2013-07-17 | 内蒙古和美科盛生物技术有限公司 | Compound lactobacillus probiotics for effectively preventing and improving bovine mastitis |
| CN103243043A (en) * | 2013-03-22 | 2013-08-14 | 内蒙古和美科盛生物技术有限公司 | Excellent bacillus coagulans for improving inferior piglet breeding and application of bacillus coagulans |
| CN103255081A (en) * | 2013-03-22 | 2013-08-21 | 内蒙古和美科盛生物技术有限公司 | Excellent bacillus coagulans for breeding sows and application thereof |
Non-Patent Citations (1)
| Title |
|---|
| 董惠钧 等: "新型微生态益生菌凝结芽孢杆菌研究进展", 《食品科学》 * |
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| CN105053566B (en) * | 2015-08-18 | 2018-09-11 | 河北大广生物科技有限公司 | A kind of bicycle beam wood seed Pepsin feed addictive and preparation method thereof |
| CN106551081A (en) * | 2016-11-09 | 2017-04-05 | 润盈生物工程（上海）有限公司 | A kind of mixed type additive for microbe feedstuff and its application |
| CN106819625A (en) * | 2016-12-19 | 2017-06-13 | 上海创博生态工程有限公司 | A kind of mandarin fish fermented feed and preparation method thereof |
| CN108669346A (en) * | 2018-05-04 | 2018-10-19 | 江苏宏业生态农业发展有限公司 | A kind of organic Se-rich chicken feed and its preparation process |
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| CN110106108A (en) * | 2019-04-25 | 2019-08-09 | 黄山市益天士生物科技有限公司 | Method for improving purifying water body breeding colony speed |
| CN115261250A (en) * | 2022-04-29 | 2022-11-01 | 江苏溢荟生物技术有限公司 | Preparation method of synbiotics for shrimp and crab intestinal health |
| CN115868581A (en) * | 2023-01-07 | 2023-03-31 | 山东省农业科学院畜牧兽医研究所 | Beef cattle fermented feed and preparation method thereof |
| CN116491577A (en) * | 2023-05-08 | 2023-07-28 | 宁夏大学 | High-activity probiotic composite microecological preparation and preparation method and application thereof |
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