CN104208118A - Traditional Chinese medicine and microecologic preparation composition for reducing somatic number of cow and preparation method thereof - Google Patents
Traditional Chinese medicine and microecologic preparation composition for reducing somatic number of cow and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a traditional Chinese medicine and microecologic preparation composition for reducing the somatic number of a cow. The composition comprises the following components in parts by weight: 60-80 parts of epimedium extract and 20-40 parts of a lactobacillus fermentation preparation. Furthermore, the invention discloses a preparation method of the traditional Chinese medicine and microecologic preparation composition for reducing the somatic number of the cow. The epimedium extract and lactobacillus fermentation preparation belong to the scopes of traditional Chinese medicine and microecologics, are advocated in China to be beneficial substitutes for such chemical products as antibiotics, and have the advantages of being safe and efficient, being free from residue, not violating a ban and the like. The composition disclosed by the invention, by indirectly enhancing immunity, directly killing bacteria and providing nutrient components, can rapidly take an effect of reducing the somatic number of the cow; moreover, the preparation method disclosed by the invention is simple and easy to operate, and the epimedium extract and the lactobacillus fermentation preparation are obtained to the greatest extent without damaging active ingredients of the epimedium extract and the lactobacillus fermentation preparation.
Description
Technical field
The present invention relates to a kind of reduce Contents in Cows cell number Chinese medicine and microbial ecological agent combination and preparation method.
Background technology
Mastitis is the first disease of puzzlement dairy, and clinic mastitis is all generally caused by latent mammitis further develops.And the increase of latent mammitis principal character somatic number often.Somatic number is higher, and latent mammitis is more serious, more easily changes into clinic mastitis.
Somatic number increases, and pathogenic bacterial infection mammary gland has been described, and milch cow immune system starts and processes pathogen, and when immune system can not remove pathogen completely, somatic number can continue rising, and milk yield significantly declines, and towards the future development of mastitis.
Now, increasing Nai Ye company starts to pay close attention to somatic number, when somatic number exceedes some in milk, can make a price reduction milk collection or refusal milk collection.Therefore the product of exploitation reduction somatic number is particularly urgent.The product reducing somatic number at present is on the market mainly Chinese powder medicine, levamisole, or even steals the antibiotic etc. added.Chinese powder medicine consumption is large, and bioavailability is low, and DeGrain, mainly to improve immunity.Levamisole has been forbidden adding in milch cow medicine.Antibiotic is prohibited especially due to residue problem.Therefore, invent a kind of product of efficient, green, noresidue, somatic number can be reduced fast, and be maintained reduced levels, thus reduce pasture and dairy farmer's loss has very high economic worth.
Summary of the invention
The present invention solves in prior art according to the performance that somatic number increases, mammitis of cow causes milk yield decline, milk industry economic benefit reduces, prior art reduces the unconspicuous technical problem of effect of drugs of somatic number, devises a kind of safety, the Chinese medicine of reduction Contents in Cows cell number of successful and microbial ecological agent combination and preparation method.
The present invention is the technical scheme realizing the employing of its object:
Reduce Chinese medicine and the microbial ecological agent combination of Contents in Cows cell number, its composition comprises: by weight, Herba Epimedii extract 60-80 part, lactic acid bacteria fermentation preparation 20-40 part.
Its composition comprises: by weight, Herba Epimedii extract 60 parts, 40 parts, lactic acid bacteria fermentation preparation.
Its composition comprises: by weight, Herba Epimedii extract 65 parts, 35 parts, lactic acid bacteria fermentation preparation.
Its composition comprises: by weight, Herba Epimedii extract 70 parts, 30 parts, lactic acid bacteria fermentation preparation.
Its composition comprises: by weight, Herba Epimedii extract 75 parts, 25 parts, lactic acid bacteria fermentation preparation.
Its composition comprises: by weight, Herba Epimedii extract 80 parts, 20 parts, lactic acid bacteria fermentation preparation.
Prepare the Chinese medicine of reduction Contents in Cows cell number according to claim 1 and the method for microbial ecological agent combination, comprise the following steps:
A: epimedium herb is pulverized, obtain granularity at 10-20 object Herba Epimedii powder, then Herba Epimedii powder is placed in 70% ethanol of 8-10 times of weight, reflux, extract, twice, each 30-60 minute, the lixiviating solution of twice reflux, extract, is merged, obtain merging lixiviating solution, utilize alcohol distillation column to reclaim ethanol, and merging lixiviating solution is concentrated in 80-100 DEG C by concentration tank, until be concentrated into the 1/15-1/10 merging lixiviating solution cumulative volume, obtain concentrated solution, then concentrated solution is carried out spraying dry, obtain Herba Epimedii extract, for subsequent use;
B: the lactic acid bacteria culturers preserved in test tube is inoculated in MRS liquid seed culture medium, Anaerobic culturel 48 hours, obtain lactobacillus liquid seeds, then the inoculum concentration of this lactobacillus liquid seeds by volume percentage ratio 0.5% is connected in liquid modified MRS culture medium, in 30-32 DEG C of anaerobic fermentation 60-72 hour, after fermentation ends, fermentation liquid is concentrated into the 1/10-1/6 of fermentation liquid original volume in 40-50 DEG C, obtain Fermented Condensed liquid, gained Fermented Condensed liquid is carried out spraying dry, obtain lactic acid bacteria fermentation preparation, for subsequent use;
C: the Herba Epimedii extract obtain steps A and step B respectively and lactic acid bacteria fermentation preparation mix in proportion, and to obtain final product.
In described steps A, granularity is at 10-20 object Herba Epimedii powder, and particle size distribution is: 100% by 10 mesh sieves, and 98.1-98.9% is not by 20 mesh sieves.
In described step B, lactic acid bacteria culturers is selected from the one in Lactobacillus plantarum, enterococcus faecalis, bacillus acidophilus's fermentation.
In described step B, the formula of MRS liquid seed culture medium is: peptone 10.0g/L, Carnis Bovis seu Bubali cream 10.0g/L, yeast extract 5.0g/L, diammonium hydrogen citrate 2.0g/L, glucose 20.0g/L, tween 80 1.0mL/L, sodium acetate 5.0g/L, potassium dihydrogen phosphate 2.0g/L, magnesium sulfate 0.58g/L, manganese sulfate 0.25g/L, solvent is distilled water, pH value 6.2 ~ 6.6; The formula of liquid modified MRS culture medium is: peptone 12.0g/L, Carnis Bovis seu Bubali cream 5.0g/L, yeast extract 6.0g/L, diammonium hydrogen citrate 2.0g/L, glucose 20.0g/L, tween 80 1.0mL/L, sodium acetate 5.0g/L, potassium dihydrogen phosphate 2.0g/L, magnesium sulfate 0.2g/L, manganese sulfate 0.05g/L, solvent is distilled water, pH value 6.2.
The invention has the beneficial effects as follows: containing the nutritional labeling such as polysaccharide and protein in Herba Epimedii extract, these compositions such as polysaccharide and albumen can as the fermentation substrate of lactobacillus, promote the fast breeding of lactobacillus, lactic acid, lactein and antibacterial peptide isoreactivity Fungicidal substance is produced in lactobacillus breeding, and the nutritional labeling such as multivitamin, after intestinal absorption, play a role rapidly.And polysaccharide composition is through lactic acid bacteria tranformation, molecular structure changes, and side effect reduces, and effect strengthens.Protein ingredient, through the Degradation of lactobacillus catabolic enzyme, generates small-molecular peptides or aminoacid, thus strengthens immunity and trophic function.
Herba Epimedii extract is also rich in the material such as icariin, flavone, by working in coordination with lactic acid bacteria fermentation preparation, can increase the quantity of macrophage in tissue, significantly strengthens the phagocytic activity of macrophage; The lymphocytic propagation of T and conversion can be strengthened; In addition, it also has obvious potentiation to NK cell, eosinophilic granulocyte, neutrophil cell, basophilic granulocyte etc.The enhancing of various immunocyte, can engulf more efficiently sooner and kill the pathogen invading mammary gland and body, thus reduce somatic number fast, improve the body immunity of milch cow, suppress somatic number.
Said composition has the immunologic function of enhancing body, directly kills the pathogen of invasion, and generates effect of the nutritional labeling three such as vitamin, aminoacid aspect, by these three kinds of effects, can reduce somatic cell fast, accelerate the body recovery of ill milch cow.
Herba Epimedii extract and lactic acid bacteria fermentation preparation are under the jurisdiction of Chinese medicine and microbial ecological agent category, are the favourable replacers of the chemical drugs products such as the substitute antibiotics advocated of country, have safe and efficient, noresidue, the advantage such as not violated.This is combined through and indirectly strengthens immunologic function, directly bactericidal action and provide nutritional labeling, and play the effect reducing Contents in Cows cell number fast, use 7-10 days continuously, effect clearly.Compared to prior art not only successful but also rapidly, by Chinese medicine and the microbial ecological agent combination of reduction Contents in Cows cell number of the present invention, greatly reduce Contents in Cows cell number.
Preparation method of the present invention is simple, easy to operate, farthest obtain Herba Epimedii extract and lactic acid bacteria fermentation preparation, do not destroy the effective ingredient of Herba Epimedii extract and lactic acid bacteria fermentation preparation, preparation parameter is wherein drawn by long-term research and strict experimental summary, crucial effect is had to the effective ingredient of this product composition and drug effect, it is the bioavailability of composite portfolio thing, toxic and side effects, the performance of physicochemical property and the rear drug effect of combination, drug level, many-sided considerations such as the suitability of milch cow and safety, and each parameter of the present invention makes somatic number in the Chinese medicine of the reduction Contents in Cows cell number obtained and microbial ecological agent Combination application to milch cow greatly reduce, compared to existing product successful and rapidly.
Detailed description of the invention
The present invention solves in prior art according to the performance that somatic number increases, mammitis of cow causes milk yield decline, milk industry economic benefit reduces, it is not obvious that prior art reduces the effect of drugs of somatic number, designs a kind of safety, the Chinese medicine of reduction Contents in Cows cell number of successful and microbial ecological agent combination and preparation method.Below in conjunction with specific embodiment, the invention will be further described.
Embodiment 1. reduces Chinese medicine and the microbial ecological agent combination of Contents in Cows cell number, and its composition comprises: by weight, Herba Epimedii extract 60 parts, 40 parts, lactic acid bacteria fermentation preparation.
Prepare the Chinese medicine of above-mentioned reduction Contents in Cows cell number and the method for microbial ecological agent combination, comprise the following steps:
A: 7kg epimedium herb is pulverized, obtain granularity at 10-20 object Herba Epimedii powder, particle size distribution is: 100% by 10 mesh sieves, 98.1% by 20 mesh sieves, then Herba Epimedii powder is placed in 70% ethanol of 8 times of weight, reflux, extract, twice, each 30-60 minute, the lixiviating solution of twice reflux, extract, is merged, obtain merging lixiviating solution 163L, alcohol distillation column is utilized to reclaim ethanol, and merging lixiviating solution is concentrated in 80 DEG C by concentration tank, until merge lixiviating solution to be concentrated into 16L, obtain concentrated solution, then concentrated solution is carried out spraying dry, obtain Herba Epimedii extract 1.38kg, for subsequent use.
B: by the Lactobacillus plantarum strain inoculation of preserving in test tube in MRS liquid seed culture medium, Anaerobic culturel 48 hours, obtain Lactobacillus plantarum liquid seeds, then the inoculum concentration of Lactobacillus plantarum liquid seeds 180mL by volume percentage ratio 0.5% is connected in liquid modified MRS culture medium, in 30 DEG C of anaerobic fermentations 62 hours, after fermentation ends, obtain fermentation liquid 34.6L, fermentation liquid is concentrated into 4.7L in 45 DEG C, obtain Fermented Condensed liquid, gained Fermented Condensed liquid is carried out spraying dry, obtains Lactobacillus plantarum fermentation preparation 0.73kg, for subsequent use.
C: the Herba Epimedii extract obtain steps A and step B respectively and Lactobacillus plantarum fermentation preparation in proportion, take Herba Epimedii extract 1.1kg, Lactobacillus plantarum fermentation preparation 0.73kg, mix homogeneously, subpackage and get final product.
Embodiment 2. reduces Chinese medicine and the microbial ecological agent combination of Contents in Cows cell number, and its composition comprises: by weight, Herba Epimedii extract 65 parts, 35 parts, lactic acid bacteria fermentation preparation.
Prepare the Chinese medicine of above-mentioned reduction Contents in Cows cell number and the method for microbial ecological agent combination, comprise the following steps:
A: 10kg epimedium herb is pulverized, obtain granularity at 10-20 object Herba Epimedii powder, particle size distribution is: 100% by 10 mesh sieves, 98.3% by 20 mesh sieves, then Herba Epimedii powder is placed in 70% ethanol of 8.5 times of weight, reflux, extract, twice, each 30-60 minute, the lixiviating solution of twice reflux, extract, is merged, obtain merging lixiviating solution 185L, alcohol distillation column is utilized to reclaim ethanol, and merging lixiviating solution is concentrated in 85 DEG C by concentration tank, until merge lixiviating solution to be concentrated into 15.1L, obtain concentrated solution, then concentrated solution is carried out spraying dry, obtain Herba Epimedii extract 1.8kg, for subsequent use.
B: by the enterococcus faecalis strain inoculation of preserving in test tube in MRS liquid seed culture medium, Anaerobic culturel 48 hours, obtain enterococcus faecalis liquid seeds, then the inoculum concentration of enterococcus faecalis liquid seeds 200mL by volume percentage ratio 0.5% is connected in liquid modified MRS culture medium, in 30 DEG C of anaerobic fermentations 64 hours, after fermentation ends, obtain fermentation liquid 39.4L, fermentation liquid is concentrated into 5.5L in 45 DEG C, obtain Fermented Condensed liquid, gained Fermented Condensed liquid is carried out spraying dry, obtains enterococcus faecalis fermentation preparation 0.87kg, for subsequent use.
C: the Herba Epimedii extract obtain steps A and step B respectively and enterococcus faecalis fermentation preparation in proportion, take Herba Epimedii extract 1.58kg, enterococcus faecalis fermentation preparation 0.85kg, mix homogeneously, subpackage and get final product.
Embodiment 3. reduces Chinese medicine and the microbial ecological agent combination of Contents in Cows cell number, and its composition comprises: by weight, Herba Epimedii extract 70 parts, 30 parts, lactic acid bacteria fermentation preparation.
Prepare the Chinese medicine of above-mentioned reduction Contents in Cows cell number and the method for microbial ecological agent combination, comprise the following steps:
A: 15kg epimedium herb is pulverized, obtain granularity at 10-20 object Herba Epimedii powder, particle size distribution is: 100% by 10 mesh sieves, 98.8% by 20 mesh sieves, then Herba Epimedii powder is placed in 70% ethanol of 10 times of weight, reflux, extract, twice, each 30-60 minute, the lixiviating solution of twice reflux, extract, is merged, obtain merging lixiviating solution 277.2L, alcohol distillation column is utilized to reclaim ethanol, and merging lixiviating solution is concentrated in 90 DEG C by concentration tank, until merge lixiviating solution to be concentrated into 20.7L, obtain concentrated solution, then concentrated solution is carried out spraying dry, obtain Herba Epimedii extract 2.7kg, for subsequent use.
B: the Lactobacillus acidophilus species preserved in test tube is inoculated in MRS liquid seed culture medium, Anaerobic culturel 48 hours, obtain bacillus acidophilus's liquid seeds, then the inoculum concentration of bacillus acidophilus's liquid seeds 250mL by volume percentage ratio 0.5% is connected in liquid modified MRS culture medium, in 30 DEG C of anaerobic fermentations 67 hours, after fermentation ends, obtain fermentation liquid 49.3L, fermentation liquid is concentrated into 5L in 48 DEG C, obtain Fermented Condensed liquid, gained Fermented Condensed liquid is carried out spraying dry, obtains bacillus acidophilus's fermentation preparation 1.2kg, for subsequent use.
C: the Herba Epimedii extract obtain steps A and step B respectively and bacillus acidophilus's fermentation preparation in proportion, take Herba Epimedii extract 2.67kg, enterococcus faecalis fermentation preparation 1.16kg, mix homogeneously, subpackage and get final product.
Embodiment 4. reduces Chinese medicine and the microbial ecological agent combination of Contents in Cows cell number, and its composition comprises: by weight, Herba Epimedii extract 75 parts, 25 parts, lactic acid bacteria fermentation preparation.
Prepare the Chinese medicine of above-mentioned reduction Contents in Cows cell number and the method for microbial ecological agent combination, comprise the following steps:
A: 12kg epimedium herb is pulverized, obtain granularity at 10-20 object Herba Epimedii powder, particle size distribution is: 100% by 10 mesh sieves, 98.5% by 20 mesh sieves, then Herba Epimedii powder is placed in 70% ethanol of 9 times of weight, reflux, extract, twice, each 30-60 minute, the lixiviating solution of twice reflux, extract, is merged, obtain merging lixiviating solution 232.3L, alcohol distillation column is utilized to reclaim ethanol, and merging lixiviating solution is concentrated in 95 DEG C by concentration tank, until merge lixiviating solution to be concentrated into 18.3L, obtain concentrated solution, then concentrated solution is carried out spraying dry, obtain Herba Epimedii extract 2.3kg, for subsequent use.
B: the Lactobacillus acidophilus species preserved in test tube is inoculated in MRS liquid seed culture medium, Anaerobic culturel 48 hours, obtain bacillus acidophilus's liquid seeds, then the inoculum concentration of bacillus acidophilus's liquid seeds 200mL by volume percentage ratio 0.5% is connected in liquid modified MRS culture medium, in 30 DEG C of anaerobic fermentations 69 hours, after fermentation ends, obtain fermentation liquid 42.7L, fermentation liquid is concentrated into 4.3L in 50 DEG C, obtain Fermented Condensed liquid, gained Fermented Condensed liquid is carried out spraying dry, obtains bacillus acidophilus's fermentation preparation 1.03kg, for subsequent use.
C: the Herba Epimedii extract obtain steps A and step B respectively and bacillus acidophilus's fermentation preparation in proportion, take Herba Epimedii extract 2.1kg, bacillus acidophilus's fermentation preparation 0.7kg, mix homogeneously, subpackage and get final product.
Embodiment 5. reduces Chinese medicine and the microbial ecological agent combination of Contents in Cows cell number, and its composition comprises: by weight, Herba Epimedii extract 80 parts, 20 parts, lactic acid bacteria fermentation preparation.
Prepare the Chinese medicine of above-mentioned reduction Contents in Cows cell number and the method for microbial ecological agent combination, comprise the following steps:
A: 13kg epimedium herb is pulverized, obtain granularity at 10-20 object Herba Epimedii powder, particle size distribution is: 100% by 10 mesh sieves, 98.7% by 20 mesh sieves, then Herba Epimedii powder is placed in 70% ethanol of 9.5 times of weight, reflux, extract, twice, each 30-60 minute, the lixiviating solution of twice reflux, extract, is merged, obtain merging lixiviating solution 245.7L, alcohol distillation column is utilized to reclaim ethanol, and merging lixiviating solution is concentrated in 100 DEG C by concentration tank, until merge lixiviating solution to be concentrated into 16.4L, obtain concentrated solution, then concentrated solution is carried out spraying dry, obtain Herba Epimedii extract 2.43kg, for subsequent use.
B: by the Lactobacillus plantarum strain inoculation of preserving in test tube in MRS liquid seed culture medium, Anaerobic culturel 48 hours, obtain Lactobacillus plantarum liquid seeds, then the inoculum concentration of Lactobacillus plantarum liquid seeds 220mL by volume percentage ratio 0.5% is connected in liquid modified MRS culture medium, in 30 DEG C of anaerobic fermentations 72 hours, after fermentation ends, obtain fermentation liquid 44.3L, fermentation liquid is concentrated into 7.3L in 48 DEG C, obtain Fermented Condensed liquid, gained Fermented Condensed liquid is carried out spraying dry, obtains Lactobacillus plantarum fermentation preparation 1.12kg, for subsequent use.
C: the Herba Epimedii extract obtain steps A and step B respectively and Lactobacillus plantarum fermentation preparation in proportion, take Herba Epimedii extract 2.4kg, Lactobacillus plantarum fermentation preparation 0.6kg, mix homogeneously, subpackage and get final product.
Choose the milch cow sample that 600 somatic numbers are higher, the average initial somatic number of milch cow is 158.2 ten thousand/mL, be divided into 6 groups at random, be respectively matched group and embodiment group, often organize 100 cow heads, to throw something and feed every day product 50g by every cow head, matched group is thrown something and fed existing product on market, embodiment group is thrown something and fed above-mentioned 5 groups of embodiment products respectively, is used in conjunction 10 days.Adopt every day CMT detection method to carry out somatic number mensuration, result is as follows:
To throw something and feed the somatic number measured value of the matched group milch cow of existing product on market, in table 1:
Table 1:
The somatic number measured value of the milch cow of embodiment of throwing something and feeding 1 product, in table 2:
Table 2:
The somatic number measured value of the milch cow of embodiment of throwing something and feeding 2 product, in table 3:
Table 3:
The somatic number measured value of the milch cow of embodiment of throwing something and feeding 3 product, in table 4:
Table 4:
The somatic number measured value of the milch cow of embodiment of throwing something and feeding 4 product, in table 5:
Table 5:
The somatic number measured value of the milch cow of embodiment of throwing something and feeding 5 product, in table 6:
Table 6:
By above-mentioned data analysis, can find out that this invention product has excellent effect in reduction Contents in Cows cell number, continuous use 10 days, somatic number can be down to about original half, the somatic product of existing reduction milch cow on market, level obviously promotes, according to the decline of Contents in Cows cell number, show that latent mammitis alleviates, its probability changing into clinic mastitis reduces greatly, the body constitution of milch cow is more healthy, and milk yield also increases, and has outstanding impact to milk industry industry.
And by contrasting above-mentioned table 1-6, we can see, when using 8 days, the fall of somatic number tends towards stability.Illustrate that this combination is 7-10 days for reducing the somatic optimal use time of milch cow.
Claims (10)
1. reduce Chinese medicine and the microbial ecological agent combination of Contents in Cows cell number, it is characterized in that: its composition comprises: by weight, Herba Epimedii extract 60-80 part, lactic acid bacteria fermentation preparation 20-40 part.
2. the Chinese medicine of reduction Contents in Cows cell number according to claim 1 and microbial ecological agent combination, is characterized in that: its composition comprises: by weight, Herba Epimedii extract 60 parts, 40 parts, lactic acid bacteria fermentation preparation.
3. the Chinese medicine of reduction Contents in Cows cell number according to claim 1 and microbial ecological agent combination, is characterized in that: its composition comprises: by weight, Herba Epimedii extract 65 parts, 35 parts, lactic acid bacteria fermentation preparation.
4. the Chinese medicine of reduction Contents in Cows cell number according to claim 1 and microbial ecological agent combination, is characterized in that: its composition comprises: by weight, Herba Epimedii extract 70 parts, 30 parts, lactic acid bacteria fermentation preparation.
5. the Chinese medicine of reduction Contents in Cows cell number according to claim 1 and microbial ecological agent combination, is characterized in that: its composition comprises: by weight, Herba Epimedii extract 75 parts, 25 parts, lactic acid bacteria fermentation preparation.
6. the Chinese medicine of reduction Contents in Cows cell number according to claim 1 and microbial ecological agent combination, is characterized in that: its composition comprises: by weight, Herba Epimedii extract 80 parts, 20 parts, lactic acid bacteria fermentation preparation.
7. prepare the Chinese medicine of reduction Contents in Cows cell number according to claim 1 and the method for microbial ecological agent combination, it is characterized in that: comprise the following steps:
A: epimedium herb is pulverized, obtain granularity at 10-20 object Herba Epimedii powder, then Herba Epimedii powder is placed in 70% ethanol of 8-10 times of weight, reflux, extract, twice, each 30-60 minute, the lixiviating solution of twice reflux, extract, is merged, obtain merging lixiviating solution, utilize alcohol distillation column to reclaim ethanol, and merging lixiviating solution is concentrated in 80-100 DEG C by concentration tank, until be concentrated into the 1/15-1/10 merging lixiviating solution cumulative volume, obtain concentrated solution, then concentrated solution is carried out spraying dry, obtain Herba Epimedii extract, for subsequent use;
B: the lactic acid bacteria culturers preserved in test tube is inoculated in MRS liquid seed culture medium, Anaerobic culturel 48 hours, obtain lactobacillus liquid seeds, then the inoculum concentration of this lactobacillus liquid seeds by volume percentage ratio 0.5% is connected in liquid modified MRS culture medium, in 30-32 DEG C of anaerobic fermentation 60-72 hour, after fermentation ends, fermentation liquid is concentrated into the 1/10-1/6 of fermentation liquid original volume in 40-50 DEG C, obtain Fermented Condensed liquid, gained Fermented Condensed liquid is carried out spraying dry, obtain lactic acid bacteria fermentation preparation, for subsequent use;
C: the Herba Epimedii extract obtain steps A and step B respectively and lactic acid bacteria fermentation preparation mix in proportion, must reduce Chinese medicine and the microbial ecological agent combination of Contents in Cows cell number.
8. the Chinese medicine of reduction Contents in Cows cell number according to claim 7 and the preparation method of microbial ecological agent combination, it is characterized in that: in described steps A, granularity is at 10-20 object Herba Epimedii powder, particle size distribution is: 100% by 10 mesh sieves, and 98.1-98.9% is not by 20 mesh sieves.
9. the Chinese medicine of reduction Contents in Cows cell number according to claim 7 and the preparation method of microbial ecological agent combination, is characterized in that: in described step B, lactic acid bacteria culturers is selected from the one in Lactobacillus plantarum, enterococcus faecalis, bacillus acidophilus's fermentation.
10. the Chinese medicine of reduction Contents in Cows cell number according to claim 7 and the preparation method of microbial ecological agent combination, it is characterized in that: in described step B, the formula of MRS liquid seed culture medium is: peptone 10.0g/L, Carnis Bovis seu Bubali cream 10.0g/L, yeast extract 5.0g/L, diammonium hydrogen citrate 2.0g/L, glucose 20.0g/L, tween 80 1.0mL/L, sodium acetate 5.0g/L, potassium dihydrogen phosphate 2.0g/L, magnesium sulfate 0.58g/L, manganese sulfate 0.25g/L, solvent is distilled water, pH value 6.2 ~ 6.6; The formula of liquid modified MRS culture medium is: peptone 12.0g/L, Carnis Bovis seu Bubali cream 5.0g/L, yeast extract 6.0g/L, diammonium hydrogen citrate 2.0g/L, glucose 20.0g/L, tween 80 1.0mL/L, sodium acetate 5.0g/L, potassium dihydrogen phosphate 2.0g/L, magnesium sulfate 0.2g/L, manganese sulfate 0.05g/L, solvent is distilled water, pH value 6.2.
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| CN103202391A (en) * | 2013-03-22 | 2013-07-17 | 内蒙古和美科盛生物技术有限公司 | Compound lactobacillus probiotics for effectively preventing and improving bovine mastitis |
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| CN103202391A (en) * | 2013-03-22 | 2013-07-17 | 内蒙古和美科盛生物技术有限公司 | Compound lactobacillus probiotics for effectively preventing and improving bovine mastitis |
Non-Patent Citations (2)
| Title |
|---|
| 冯金英等: "奶牛乳腺炎的防治", 《农村实用科技信息》 * |
| 高鹏飞: "复合乳酸菌制剂在防治奶牛***炎方面的应用研究", 《中国畜牧杂志》 * |
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Denomination of invention: Combination and preparation method of traditional Chinese medicine and microecological preparation for reducing the body cell number of dairy cows Effective date of registration: 20230221 Granted publication date: 20180306 Pledgee: Bank of Hebei Co.,Ltd. Baoding Gaoyang Sub branch Pledgor: BAODING JIZHONG PHARMACEUTICAL Co.,Ltd. Registration number: Y2023980033071 |