CN105368749A - Bacillus subtilis and feed additive containing same - Google Patents
Bacillus subtilis and feed additive containing same Download PDFInfo
- Publication number
- CN105368749A CN105368749A CN201510891728.2A CN201510891728A CN105368749A CN 105368749 A CN105368749 A CN 105368749A CN 201510891728 A CN201510891728 A CN 201510891728A CN 105368749 A CN105368749 A CN 105368749A
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- Prior art keywords
- bacillus subtilis
- subtilis
- feed
- experimental group
- feed additive
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- 244000063299 Bacillus subtilis Species 0.000 title abstract description 11
- 235000014469 Bacillus subtilis Nutrition 0.000 title abstract description 9
- 239000003674 animal food additive Substances 0.000 title abstract 2
- 239000000654 additive Substances 0.000 claims description 15
- 102000004190 Enzymes Human genes 0.000 claims description 10
- 108090000790 Enzymes Proteins 0.000 claims description 10
- 241000411851 herbal medicine Species 0.000 claims description 3
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- 229940088594 vitamin Drugs 0.000 claims description 3
- 229930003231 vitamin Natural products 0.000 claims description 3
- 235000013343 vitamin Nutrition 0.000 claims description 3
- 239000003995 emulsifying agent Substances 0.000 claims description 2
- 150000004676 glycans Chemical class 0.000 claims description 2
- 229920001282 polysaccharide Polymers 0.000 claims description 2
- 239000005017 polysaccharide Substances 0.000 claims description 2
- 150000003722 vitamin derivatives Chemical class 0.000 claims description 2
- 241001465754 Metazoa Species 0.000 abstract description 19
- 230000012010 growth Effects 0.000 abstract description 15
- 108091005804 Peptidases Proteins 0.000 abstract description 11
- 102000035195 Peptidases Human genes 0.000 abstract description 10
- 239000004382 Amylase Substances 0.000 abstract description 6
- 102000013142 Amylases Human genes 0.000 abstract description 6
- 108010065511 Amylases Proteins 0.000 abstract description 6
- 235000019418 amylase Nutrition 0.000 abstract description 6
- 201000010099 disease Diseases 0.000 abstract description 6
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract description 6
- 244000052616 bacterial pathogen Species 0.000 abstract description 5
- 241000544286 Vibrio anguillarum Species 0.000 abstract description 4
- 238000009360 aquaculture Methods 0.000 abstract description 4
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- 230000036039 immunity Effects 0.000 abstract description 4
- 238000012216 screening Methods 0.000 abstract description 4
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- 230000002401 inhibitory effect Effects 0.000 abstract 1
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- 241000252230 Ctenopharyngodon idella Species 0.000 description 14
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- 102000004169 proteins and genes Human genes 0.000 description 6
- 108090000623 proteins and genes Proteins 0.000 description 6
- 210000002966 serum Anatomy 0.000 description 6
- 235000019786 weight gain Nutrition 0.000 description 6
- 229920001817 Agar Polymers 0.000 description 5
- 239000001888 Peptone Substances 0.000 description 5
- 108010080698 Peptones Proteins 0.000 description 5
- 239000008272 agar Substances 0.000 description 5
- 239000012153 distilled water Substances 0.000 description 5
- 230000002255 enzymatic effect Effects 0.000 description 5
- 230000003203 everyday effect Effects 0.000 description 5
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- 230000001954 sterilising effect Effects 0.000 description 5
- 102000002260 Alkaline Phosphatase Human genes 0.000 description 4
- 108020004774 Alkaline Phosphatase Proteins 0.000 description 4
- 102000008299 Nitric Oxide Synthase Human genes 0.000 description 4
- 108010021487 Nitric Oxide Synthase Proteins 0.000 description 4
- 102000019197 Superoxide Dismutase Human genes 0.000 description 4
- 108010012715 Superoxide dismutase Proteins 0.000 description 4
- 102000003929 Transaminases Human genes 0.000 description 4
- 108090000340 Transaminases Proteins 0.000 description 4
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- 239000000243 solution Substances 0.000 description 4
- 239000006228 supernatant Substances 0.000 description 4
- 241000251468 Actinopterygii Species 0.000 description 3
- 108010064696 N,O-diacetylmuramidase Proteins 0.000 description 3
- 229920002472 Starch Polymers 0.000 description 3
- 230000000844 anti-bacterial effect Effects 0.000 description 3
- 238000004820 blood count Methods 0.000 description 3
- 230000007062 hydrolysis Effects 0.000 description 3
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- 230000001717 pathogenic effect Effects 0.000 description 3
- 238000002360 preparation method Methods 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- 239000013535 sea water Substances 0.000 description 3
- 239000008107 starch Substances 0.000 description 3
- 235000019698 starch Nutrition 0.000 description 3
- 229940032362 superoxide dismutase Drugs 0.000 description 3
- 241000233866 Fungi Species 0.000 description 2
- 108090001060 Lipase Proteins 0.000 description 2
- 102000004882 Lipase Human genes 0.000 description 2
- 239000004367 Lipase Substances 0.000 description 2
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 2
- DPDMMXDBJGCCQC-UHFFFAOYSA-N [Na].[Cl] Chemical compound [Na].[Cl] DPDMMXDBJGCCQC-UHFFFAOYSA-N 0.000 description 2
- 230000003042 antagnostic effect Effects 0.000 description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- 230000003115 biocidal effect Effects 0.000 description 2
- 238000009395 breeding Methods 0.000 description 2
- 230000001488 breeding effect Effects 0.000 description 2
- 229940041514 candida albicans extract Drugs 0.000 description 2
- 239000005018 casein Substances 0.000 description 2
- BECPQYXYKAMYBN-UHFFFAOYSA-N casein, tech. Chemical compound NCCCCC(C(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(CC(C)C)N=C(O)C(CCC(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(C(C)O)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(COP(O)(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(N)CC1=CC=CC=C1 BECPQYXYKAMYBN-UHFFFAOYSA-N 0.000 description 2
- 235000021240 caseins Nutrition 0.000 description 2
- 210000002249 digestive system Anatomy 0.000 description 2
- 239000001963 growth medium Substances 0.000 description 2
- 230000003301 hydrolyzing effect Effects 0.000 description 2
- 235000019421 lipase Nutrition 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- 244000052769 pathogen Species 0.000 description 2
- 238000005728 strengthening Methods 0.000 description 2
- 239000012138 yeast extract Substances 0.000 description 2
- JLPULHDHAOZNQI-ZTIMHPMXSA-N 1-hexadecanoyl-2-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phosphocholine Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](COP([O-])(=O)OCC[N+](C)(C)C)OC(=O)CCCCCCC\C=C/C\C=C/CCCCC JLPULHDHAOZNQI-ZTIMHPMXSA-N 0.000 description 1
- 235000017060 Arachis glabrata Nutrition 0.000 description 1
- 244000105624 Arachis hypogaea Species 0.000 description 1
- 235000010777 Arachis hypogaea Nutrition 0.000 description 1
- 235000018262 Arachis monticola Nutrition 0.000 description 1
- 229920002307 Dextran Polymers 0.000 description 1
- 208000035240 Disease Resistance Diseases 0.000 description 1
- 235000019733 Fish meal Nutrition 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 244000068988 Glycine max Species 0.000 description 1
- 235000010469 Glycine max Nutrition 0.000 description 1
- 108010014251 Muramidase Proteins 0.000 description 1
- 102000016943 Muramidase Human genes 0.000 description 1
- 108010062010 N-Acetylmuramoyl-L-alanine Amidase Proteins 0.000 description 1
- 238000012408 PCR amplification Methods 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 244000046052 Phaseolus vulgaris Species 0.000 description 1
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 1
- 210000001015 abdomen Anatomy 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 210000003484 anatomy Anatomy 0.000 description 1
- 230000008485 antagonism Effects 0.000 description 1
- 230000002785 anti-thrombosis Effects 0.000 description 1
- 230000010100 anticoagulation Effects 0.000 description 1
- 229960004676 antithrombotic agent Drugs 0.000 description 1
- 230000003385 bacteriostatic effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 210000000601 blood cell Anatomy 0.000 description 1
- 210000000988 bone and bone Anatomy 0.000 description 1
- 210000003419 coelomocyte Anatomy 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 239000008367 deionised water Substances 0.000 description 1
- 229910021641 deionized water Inorganic materials 0.000 description 1
- 230000006866 deterioration Effects 0.000 description 1
- YXVFQADLFFNVDS-UHFFFAOYSA-N diammonium citrate Chemical compound [NH4+].[NH4+].[O-]C(=O)CC(O)(C(=O)O)CC([O-])=O YXVFQADLFFNVDS-UHFFFAOYSA-N 0.000 description 1
- 102000038379 digestive enzymes Human genes 0.000 description 1
- 108091007734 digestive enzymes Proteins 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000006862 enzymatic digestion Effects 0.000 description 1
- 235000021323 fish oil Nutrition 0.000 description 1
- 239000004467 fishmeal Substances 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 210000000087 hemolymph Anatomy 0.000 description 1
- 230000036737 immune function Effects 0.000 description 1
- 229960001438 immunostimulant agent Drugs 0.000 description 1
- 239000003022 immunostimulating agent Substances 0.000 description 1
- 230000003308 immunostimulating effect Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 230000000968 intestinal effect Effects 0.000 description 1
- 230000006799 invasive growth in response to glucose limitation Effects 0.000 description 1
- 229910000398 iron phosphate Inorganic materials 0.000 description 1
- WBJZTOZJJYAKHQ-UHFFFAOYSA-K iron(3+) phosphate Chemical group [Fe+3].[O-]P([O-])([O-])=O WBJZTOZJJYAKHQ-UHFFFAOYSA-K 0.000 description 1
- 235000019626 lipase activity Nutrition 0.000 description 1
- 239000004325 lysozyme Substances 0.000 description 1
- 229960000274 lysozyme Drugs 0.000 description 1
- 235000010335 lysozyme Nutrition 0.000 description 1
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 1
- 235000019341 magnesium sulphate Nutrition 0.000 description 1
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 235000012054 meals Nutrition 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 244000005706 microflora Species 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 210000000496 pancreas Anatomy 0.000 description 1
- 235000020232 peanut Nutrition 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 239000002504 physiological saline solution Substances 0.000 description 1
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 description 1
- 229920000053 polysorbate 80 Polymers 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 235000019419 proteases Nutrition 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000001632 sodium acetate Substances 0.000 description 1
- 235000017281 sodium acetate Nutrition 0.000 description 1
- 229940083466 soybean lecithin Drugs 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
Landscapes
- Enzymes And Modification Thereof (AREA)
- Fodder In General (AREA)
- Feed For Specific Animals (AREA)
Abstract
Description
Project | Control group | Experimental group |
Rate of body weight gain % | 17.42±0..68 a | 22.09+1.36 b |
N,O-Diacetylmuramidase U/L | 972.12±14.25 b | 1012.32±12.36 b |
Alkaline phosphatase U/L | 61.21±3.62 a | 67.65±4.23 b |
Glutamic-oxal(o)acetic transaminase U/L | 69.26±5.34 | 71.98±5.46 |
Gpt U/L | 9.23±1.46 a | 16.65±2.13 b |
Protease activity U/mg Protein | 23.996±1.124 a | 29.569±1.147 b |
Amylase activity U/mg Protein | 58.455±2.192 a | 99.432±2.420 b |
Lipase activity U/mg Protein | 25.537±1.595 | 27.115±0.570 |
Project | Control group | Experimental group |
Rate of body weight gain % | 116.89±1.99 a | 137.08±6.73 b |
Specific growth rate % | 1.17±0.01 a | 1.28±0.02 b |
Blood cell count 10 7cell/ml | 1.74±0.072 a | 2.408±0.095 b |
Respiratory burst vigor OD630/10 6cells | 1.415±0.038 a | 1.623±0.047 b |
Phenoloxidase Activities U/ml | 50.783±2.219 a | 72.594±3.978 b |
Superoxide dismutase activity U/ml | 41.805±0.643 | 43.395±0.469 |
Nitric oxide synthase U/ml | 4.843±0.088 a | 5.878±0.136 b |
Proteinase activity U/mg Protein | 0.08±0.0012 a | 0.13±0.0043 b |
Amylase activity U/mg Protein | 0.71±0.014 a | 1.22±0.057 b |
Claims (4)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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CN201510891728.2A CN105368749B (en) | 2015-12-04 | 2015-12-04 | One bacillus subtilis and feed addictive comprising the bacterial strain |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201510891728.2A CN105368749B (en) | 2015-12-04 | 2015-12-04 | One bacillus subtilis and feed addictive comprising the bacterial strain |
Publications (2)
Publication Number | Publication Date |
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CN105368749A true CN105368749A (en) | 2016-03-02 |
CN105368749B CN105368749B (en) | 2018-10-16 |
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CN201510891728.2A Active CN105368749B (en) | 2015-12-04 | 2015-12-04 | One bacillus subtilis and feed addictive comprising the bacterial strain |
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CN (1) | CN105368749B (en) |
Cited By (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN108977387A (en) * | 2018-08-08 | 2018-12-11 | 广东绿百多生物开发有限公司 | A kind of bacterial strain for fermented feed |
CN110029074A (en) * | 2019-03-15 | 2019-07-19 | 河南科技大学 | A kind of bacillus subtilis and its application in raising fish and shrimp disease prevention and cure |
CN111690558A (en) * | 2020-05-31 | 2020-09-22 | 青岛玛斯特生物技术有限公司 | Bacillus subtilis strain and application thereof in aquaculture |
CN112239735A (en) * | 2020-09-18 | 2021-01-19 | 华中农业大学 | Bacillus subtilis, microbial inoculum, screening method and application |
CN112715749A (en) * | 2020-11-11 | 2021-04-30 | 广西海洋研究所有限责任公司 | Trachinotus ovatus feed additive capable of improving immunity |
CN116286447A (en) * | 2022-10-09 | 2023-06-23 | 集美大学 | Bacillus subtilis and application thereof |
CN117487719A (en) * | 2023-11-25 | 2024-02-02 | 山东省海洋科学研究院(青岛国家海洋科学研究中心) | Bacillus with vibrio-resistant activity separated from intestinal tracts of prawns |
CN117683687A (en) * | 2023-12-19 | 2024-03-12 | 中国热带农业科学院热带生物技术研究所 | Bacillus-W2Z, application thereof and feed |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103834596A (en) * | 2014-03-10 | 2014-06-04 | 上海海洋大学 | Bacillus subtilis shou003, anti-vibrio protein and preparation method and applications of bacillus subtilis shou003 and anti-vibrio protein |
-
2015
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Patent Citations (1)
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CN103834596A (en) * | 2014-03-10 | 2014-06-04 | 上海海洋大学 | Bacillus subtilis shou003, anti-vibrio protein and preparation method and applications of bacillus subtilis shou003 and anti-vibrio protein |
Non-Patent Citations (1)
Title |
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徐长安,等: "海洋源生防细菌LHB02的筛选_鉴定及其抑菌谱检测", 《海洋与湖泊》 * |
Cited By (13)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN108977387A (en) * | 2018-08-08 | 2018-12-11 | 广东绿百多生物开发有限公司 | A kind of bacterial strain for fermented feed |
CN108977387B (en) * | 2018-08-08 | 2022-04-15 | 广东绿百多生物开发有限公司 | Bacterial strain for fermenting feed |
CN110029074A (en) * | 2019-03-15 | 2019-07-19 | 河南科技大学 | A kind of bacillus subtilis and its application in raising fish and shrimp disease prevention and cure |
CN110029074B (en) * | 2019-03-15 | 2020-12-29 | 河南科技大学 | Bacillus subtilis and application thereof in prevention and treatment of fish and shrimp culture diseases |
CN111690558B (en) * | 2020-05-31 | 2022-04-08 | 青岛玛斯特生物技术有限公司 | Bacillus subtilis strain and application thereof in aquaculture |
CN111690558A (en) * | 2020-05-31 | 2020-09-22 | 青岛玛斯特生物技术有限公司 | Bacillus subtilis strain and application thereof in aquaculture |
CN112239735A (en) * | 2020-09-18 | 2021-01-19 | 华中农业大学 | Bacillus subtilis, microbial inoculum, screening method and application |
CN112239735B (en) * | 2020-09-18 | 2022-05-24 | 华中农业大学 | Bacillus subtilis, microbial inoculum, screening method and application |
CN112715749A (en) * | 2020-11-11 | 2021-04-30 | 广西海洋研究所有限责任公司 | Trachinotus ovatus feed additive capable of improving immunity |
CN116286447A (en) * | 2022-10-09 | 2023-06-23 | 集美大学 | Bacillus subtilis and application thereof |
CN117487719A (en) * | 2023-11-25 | 2024-02-02 | 山东省海洋科学研究院(青岛国家海洋科学研究中心) | Bacillus with vibrio-resistant activity separated from intestinal tracts of prawns |
CN117487719B (en) * | 2023-11-25 | 2024-04-23 | 山东省海洋科学研究院(青岛国家海洋科学研究中心) | Bacillus with vibrio-resistant activity separated from intestinal tracts of prawns |
CN117683687A (en) * | 2023-12-19 | 2024-03-12 | 中国热带农业科学院热带生物技术研究所 | Bacillus-W2Z, application thereof and feed |
Also Published As
Publication number | Publication date |
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CN105368749B (en) | 2018-10-16 |
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