CN103191159B - Inflammation-diminishing bacterium-inhibition preparation and its preparation method and use - Google Patents
Inflammation-diminishing bacterium-inhibition preparation and its preparation method and use Download PDFInfo
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Abstract
The invention discloses an inflammation-diminishing bacterium-inhibition preparation. The inflammation-diminishing bacterium-inhibition preparation contains active components of 0.1-5g/100g of total ketones, 15-40g/100g of total saponins, and 45-80g/100g of triterpene. The invention also discloses a preparation method of the inflammation-diminishing bacterium-inhibition preparation, and use of the inflammation-diminishing bacterium-inhibition preparation in oral health products such as toothpaste, Koujiesu and mouthwash, a personal cleaning care liquid, a vagina bacterium-inhibition lubricating liquid, a soap sheet, itching-relieving dew, body wash, shampoo and skin care cream. The inflammation-diminishing bacterium-inhibition preparation has the characteristics of high efficiency, wide spectrum, drug resistance, rich drug sources and high safety coefficient and has obvious effects of inflammation diminishing and bacterium inhibition.
Description
Technical field
The present invention relates to field of medicaments, particularly a kind of antiinflammatory inhibiting-bacteria preparation and its preparation method and application.
Background technology
In clinical position, bacterial infection disease is the most common, and using antibiotic therapy bacterial infection disease has been one of the most general Therapeutic Method.In recent years, due to the abuse of the mankind for antibiotics, antibacterial presents fast rise trend to the drug resistance of each antibiotics, Drug resistance, persister always, even produce the situation that " powerful antibacterial " pasts medical help, and the toxic and side effects of medicine is also along with increase.
China abuses one of the most serious country of antibiotics in the world, and the bacterial drug resistance problem causing is thus particularly outstanding.Some antibacterials of clinical separation are occupied first place in the world to the drug resistance of some drugs.For example, in the staphylococcus aureus that infect in Shanghai, 80% is own through having produced the drug resistance to benzylpenicillin.The resistant rate of fluoroquinolones antibiotics has reached 60% ~ 70%.Many conventional antibiotics antibacterial efficacies day by day lower, and antibiotics drug resistance has become the difficult problem that global public health faces.World Health Organization (WHO) (World Health Organization) claims, faces the antibiotics drug resistance problem having increased rapidly since nearly half a century, and medical circle is felt simply helpless.
In addition, a plurality of positions of human body are if skin, oral cavity, vagina etc. are owing to exposing or being partly exposed to body surface, are easily subject to the infection of antibacterial, other microorganisms or biting of insecticide and cause disease or discomfort.But this situation is as used antibiotics unfavorable, if it is safer to use some externals to have antiinflammatory antibacterial medicines.
In the face of day by day serious bacterial drug resistance problem, reduce the generation of Resistant strain and develop the important topic that antibacterial-anti-inflammatory drug that new safety coefficient is high becomes 21 century medical circle.Chinese medicine is a great treasure-house, and China working people has summed up for a long time the rich experiences of a whole set of disease preventing and treating in the practice process combating the disease for thousands of years.Before modernization antibiotics is born, Chinese medicine is embodying its distinctive effect aspect some sterilizations, antiinflammatory.Based on Chinese medical theory, the Chinese medicine of most of clearing away heat-fires, clearing away heat and cooling blood, heat-clearing and toxic substances removing, cooling blood for hemostasis all have varying degree to kill (pressing down) bacterium active, and be widely used in clinically, and obtaining some achievements aspect the infection for the treatment of antibacterial.Due to the particularity of Chinese medicine, antibacterial is less produces drug resistance to Chinese medicine.
Therefore, that research has is efficient, wide spectrum, low drug resistance and the high Chinese medicine of safety coefficient, for treatment bacterial inflammation, has strong realistic meaning.
Summary of the invention
Embodiment of the present invention technical problem to be solved is, provides a kind of efficient, wide spectrum, low drug resistance, medicine source is abundant and the high antiinflammatory inhibiting-bacteria preparation of safety coefficient.
Embodiment of the present invention technical problem to be solved is also, provides a kind of efficient, wide spectrum, low drug resistance, medicine source is abundant and the preparation method of the antiinflammatory inhibiting-bacteria preparation that safety coefficient is high.
Embodiment of the present invention technical problem to be solved is also, the application of above-mentioned antiinflammatory inhibiting-bacteria preparation is provided, especially at toothpaste, mouth cleaner, collutory oral health care product, individual's conditioning liquid for cleaning, the antibacterial lubricating fluid of vagina, the application of soap sheet, itch-stopping dew, bath gel, shampoo and beneficial skin frost daily chemical products.
For reaching above-mentioned technique effect, the embodiment of the present invention provides a kind of antiinflammatory inhibiting-bacteria preparation, and it mainly contains following active component:
Total ketone 0.1 ~ 5g/100g
Total Saponin 15 ~ 40g/100g
Triterpene 45 ~ 80mg/100g.
As the improvement of such scheme, described antiinflammatory inhibiting-bacteria preparation mainly contains following active component:
Total ketone 2.43g/100g
Total Saponin 28.3g/100g
Triterpene 62.2mg/100g.
As the improvement of such scheme, the main active of described antiinflammatory inhibiting-bacteria preparation is extracted and is obtained from Ilex rotunda Thunb..
As the improvement of such scheme, described total ketone is total flavones.
Correspondingly, the invention also discloses a kind of preparation method of antiinflammatory inhibiting-bacteria preparation, comprising:
Ilex rotunda Thunb. bark is smashed to pieces, obtained Ilex rotunda Thunb. bark pieces;
By Ilex rotunda Thunb. bark pieces described in soak with ethanol, and carry out reflux, extract;
Ilex rotunda Thunb. active component concentrated extract by described reflux, extract,, obtains head product;
By described head product washing, filter;
The described head product through washing filtering is carried out to dried, obtain final products antiinflammatory inhibiting-bacteria preparation;
Described antiinflammatory inhibiting-bacteria preparation, it mainly contains following active component:
Total ketone 0.1 ~ 5g/100g
Total Saponin 15 ~ 40g/100g
Triterpene 45 ~ 80mg/100g.
As the improvement of such scheme, described by Ilex rotunda Thunb. bark pieces described in soak with ethanol, and carry out in the step of reflux, extract,, the concentration of ethanol is 70 ~ 85%.
As the improvement of such scheme, described by described head product washing, in the step of filtration, described head product adds 2 ~ 4 times of water washings.
As the improvement of such scheme, described antiinflammatory inhibiting-bacteria preparation mainly contains following active component:
Total ketone 2.43g/100g
Total Saponin 28.3g/100g
Triterpene 62.2mg/100g.
As the improvement of such scheme, described total ketone is total flavones.
Correspondingly, the present invention also provides the application of above-mentioned antiinflammatory inhibiting-bacteria preparation in toothpaste, and the addition of described antiinflammatory inhibiting-bacteria preparation in toothpaste is 0.5 ~ 2.0%.
It should be noted that, the addition of described antiinflammatory inhibiting-bacteria preparation in toothpaste is weight percentage 0.5 ~ 2.0%.
Correspondingly, the present invention also provides the application of above-mentioned antiinflammatory inhibiting-bacteria preparation in mouth cleaner, collutory oral health care product, and the addition of described antiinflammatory inhibiting-bacteria preparation in mouth cleaner, collutory oral health care product is 0.5 ~ 2.0%.
It should be noted that, the addition of described antiinflammatory inhibiting-bacteria preparation in mouth cleaner, collutory oral health care product is weight percentage 0.5 ~ 2.0%.
Correspondingly, the present invention also provides the application of above-mentioned antiinflammatory inhibiting-bacteria preparation in individual conditioning liquid for cleaning, the antibacterial lubricating fluid of vagina, and the addition of described antiinflammatory inhibiting-bacteria preparation in individual conditioning liquid for cleaning, the antibacterial lubricating fluid of vagina is 0.5 ~ 2.0%.
It should be noted that, the addition of described antiinflammatory inhibiting-bacteria preparation in individual conditioning liquid for cleaning, the antibacterial lubricating fluid of vagina is weight percentage 0.5 ~ 2.0%.
Correspondingly, the present invention also provides the application of above-mentioned antiinflammatory inhibiting-bacteria preparation in soap sheet, itch-stopping dew, bath gel, shampoo and beneficial skin frost daily chemical products, and the addition of described antiinflammatory inhibiting-bacteria preparation in soap sheet, itch-stopping dew, bath gel, shampoo and beneficial skin frost daily chemical products is 0.5 ~ 2.0%.
It should be noted that, the addition of described antiinflammatory inhibiting-bacteria preparation in soap sheet, itch-stopping dew, bath gel, shampoo and beneficial skin frost daily chemical products is weight percentage 0.5 ~ 2.0%.
Implement the present invention and there is following beneficial effect:
One, antiinflammatory inhibiting-bacteria preparation of the present invention is the active component extracting from holly plant Ilex rotunda Thunb. (having another name called Cortex Ilicis Rotundae), and active component is the total ketone of 0.1 ~ 5g/100g, the total Saponin of 15 ~ 40g/100g, 45 ~ 80mg/100g triterpene.Above-mentioned active component has heat-clearing and toxic substances removing, the antibacterial effect of antiinflammatory.Therefore, antiinflammatory inhibiting-bacteria preparation of the present invention have efficiently, wide spectrum, low drug resistance and safety coefficient high.
Two, the preparation method of antiinflammatory inhibiting-bacteria preparation of the present invention is mainly by the effective active composition in reflux, extract, natural plants Ilex rotunda Thunb., and its medicine source is abundant, simple to operate, with low cost, applied range.
Three, the present invention is applied to the series of products of daily life by the antiinflammatory inhibiting-bacteria preparation that contains total ketone, total Saponin, triterpene isoreactivity composition, comprising: toothpaste; The oral health care product such as mouth cleaner, collutory; Individual's conditioning liquid for cleaning, the antibacterial lubricating fluid of vagina; The daily chemical products such as soap sheet, itch-stopping dew, bath gel, shampoo and beneficial skin frost.And antiinflammatory inhibiting-bacteria preparation be take addition and is made an addition in above-mentioned articles for use as 0.5 ~ 2.0%.
Because antiinflammatory inhibiting-bacteria preparation has efficiently, wide spectrum, low drug resistance, medicine source is abundant and safety coefficient high.So, described in contain antiinflammatory inhibiting-bacteria preparation articles for use safe and reliable, antiinflammatory fungistatic effect is obvious.
Accompanying drawing explanation
Fig. 1 is the flow chart of the preparation method of a kind of antiinflammatory inhibiting-bacteria preparation of the present invention.
The ameba experiment schematic diagram that Fig. 2 A, Fig. 2 B, Fig. 2 C, Fig. 2 D are toothpaste of the present invention;
Fig. 3 A, Fig. 3 B, Fig. 3 C, Fig. 3 D, Fig. 3 E, Fig. 3 F for experimenter adopts the culture of bacteria of soap sheet of the present invention before and after washing one's hands remove schematic diagram;
Fig. 4 is the antipruritic effect block diagram of itch-stopping dew of the present invention.
The specific embodiment
For making the object, technical solutions and advantages of the present invention clearer, below the present invention is described in further detail.
The embodiment of the present invention provides a kind of antiinflammatory inhibiting-bacteria preparation, and it mainly contains following active component:
Total ketone 0.1 ~ 5g/100g
Total Saponin 15 ~ 40g/100g
Triterpene 45 ~ 80mg/100g.
Preferably, described antiinflammatory inhibiting-bacteria preparation mainly contains following active component:
Total ketone 2 ~ 3g/100g
Total Saponin 25 ~ 32g/100g
Triterpene 60 ~ 68mg/100g.
More preferably, described antiinflammatory inhibiting-bacteria preparation mainly contains following active component:
Total ketone 2.43g/100g
Total Saponin 28.3g/100g
Triterpene 62.2mg/100g.
Above-mentioned total ketone is total flavones.
It should be noted that, what antiinflammatory inhibiting-bacteria preparation of the present invention can be for medicated powder, ointment, spray, patch etc., but be not limited to this.
Further, the main active of described antiinflammatory inhibiting-bacteria preparation is extracted and is obtained from Ilex rotunda Thunb..
Ilex rotunda Thunb. (having another name called Cortex Ilicis Rotundae) is evergreen shrubs or arbor, 20 meters of Gao Keda, and the diameter of a cross-section of a tree trunk 1.3 meters above the ground reaches 1 meter; Bark Lycoperdon polymorphum Vitt is to grey black.Sprig is cylindrical, straight and upright, old branch tool longitudinal crevasse, and leaf scar obovate or triangle, slightly swell, and hole skin is not obvious, and the vertical rib of raw sprout tool, without hair, rare by micro-pubescence then; Terminal bud is conical.Be born in the hillside evergreen broadleaf forest of height above sea level 400-1100 rice and border.The weather of happiness warm and moist.
Ilex rotunda Thunb. medicinal part: bark or root bark with plant are used as medicine.
Ilex rotunda Thunb. effect: heat-clearing and toxic substances removing; Dampness removing; Pain relieving; Cold, fever; Tonsillitis; Laryngopharynx swelling and pain; Acute, chronic hepatitis; Acute gastroenteritis; Gastric and duodenal ulcers; Administration's diarrhea due to damp pathogen rushes down; Jaundice; Dysentery; Traumatic injury; Rheumatic arthralgia; Eczema; Furuncle.
The active component that antiinflammatory inhibiting-bacteria preparation of the present invention mainly extracts from the dry bark of holly plant Ilex rotunda Thunb., detects containing total ketone 0.1 ~ 5g/100g, total Saponin 15 ~ 40g/100g, triterpene 45 ~ 80mg/100g through China Guangzhou Analysis &. Test Center.Above-mentioned active component has heat-clearing and toxic substances removing, the antibacterial effect of antiinflammatory.
Below in conjunction with experimental data or detection data, antiinflammatory inhibiting-bacteria preparation of the present invention is further elaborated.
One, the present invention is carried out the mensuration of antiinflammatory fungistatic effect, specific as follows:
1, aerobe fungistatic effect is measured:
Through Nat'l Pharmaceutical & Biological Products Control Institute's probation redport (calibrating SJMS92591), use antiinflammatory inhibiting-bacteria preparation of the present invention, the bacterial strains such as the staphylococcus aureus of respectively oneself being known, shigella flexneri, escherichia coli, dysentery bacterium, streptococcus faecalis are done fungistatic effect test, and result of the test is as follows:
| Strains tested | Staphylococcus aureus | Shigella flexneri | Escherichia coli | Dysentery bacterium | Streptococcus faecalis |
| Inhibition zone diameter (mm) | 10.5 | 10.0 | 10.2 | 10.3 | 10.5 |
It should be noted that, above-mentioned bacterial strains is test strain, without specificity requirement, and can obtain by conventional method separation.
The measurement result of minimum inhibitory concentration is as follows:
| Strains tested | Staphylococcus aureus | Shigella flexneri | Escherichia coli | Dysentery bacterium | Streptococcus faecalis |
| Minimum inhibitory concentration (mg/ml) | 50 | ﹥100 | 50 | ﹥100 | 50 |
Result: antiinflammatory inhibiting-bacteria preparation of the present invention, has good fungistatic effect, and measured minimum inhibitory concentration all more than 10mm above-mentioned bacterial strains inhibition zone diameter.
2, anaerobe fungistatic effect is measured
Through Nat'l Pharmaceutical & Biological Products Control Institute's probation redport (SJMS92579), use antiinflammatory inhibiting-bacteria preparation of the present invention to do fungistatic effect test to the bacillus perfringens of anaerobism, clostridium tetani, the black bacteroid of product, bacteroides fragilis, propionibacterium acnes and common Candida albicans etc., result of the test is as follows:
| Strains tested | Bacillus perfringens | Clostridium tetani | Produce black bacteroid | Bacteroides fragilis | Propionibacterium acnes | Candida albicans |
| Inhibition zone diameter (mm) | 12.0 | 10.7 | 10.2 | 11.5 | 10.5 | 10.0 |
It should be noted that, above-mentioned bacterial strains is test strain, without specificity requirement, and can obtain by conventional method separation.
The measurement result of minimum inhibitory concentration is as follows:
| Strains tested | Bacillus perfringens | Clostridium tetani | Produce black bacteroid | Bacteroides fragilis | Propionibacterium acnes | Candida albicans |
| Minimum inhibitory concentration (mg/ml) | 25 | 25 | 25 | 100 | 100 | 50 |
Result: antiinflammatory inhibiting-bacteria preparation of the present invention, has good fungistatic effect, and measured minimum inhibitory concentration all more than 10mm above-mentioned bacterial strains inhibition zone diameter.
Two, nutrient is measured:
This product detects and contains 16 kinds, aminoacid through China Guangzhou Analysis &. Test Center, be respectively aspartic acid, glutamic acid, serine, histidine, glycine, threonine, arginine, alanine, tyrosine, valine, methionine, phenylalanine, isoleucine, leucine, lysine, proline, total content 91.4mg/100g; Polysaccharide 2.67g/100ml.And contain vitamin B1, B2, C, K etc., especially take vitamin B1, B2 as many.
Result: antiinflammatory inhibiting-bacteria preparation of the present invention contains above-mentioned nutrient, has certain effect to strengthening skin, mucosa resistance and nutrition skin.
Three, security test:
Antiinflammatory inhibiting-bacteria preparation of the present invention has good antiinflammatory fungistatic effect after tested, but whether in application process, must should be noted that safety, therefore, has done following safety toxicity test, comprising: acute toxicity test, local skin test, mucosa stimulation test.
Result: above-mentioned toxicological test proved invention antiinflammatory inhibiting-bacteria preparation be a kind of harmless, nontoxic, have no side effect, non-stimulated large article of safety coefficient.
1, acute toxicity test
Antiinflammatory inhibiting-bacteria preparation of the present invention is used to 18-20 gram of ♂ ♀ half and half of kunming mice body weight, by abdomen injection and administration by gavage, test.
Result:
A, intraperitoneal injection:
LD
50=2.022g/kg
Average fiducial limit=2.022 ± 0.1545k/kg
B, administration by gavage:
By the 2 times/day of gavages of antiinflammatory inhibiting-bacteria preparation of the present invention that are greater than ten multiple doses, no abnormality seen reaction.
2, tuerculoderma
The present invention that use is made medicated powder and made 2%, 5% ointment carries out irritating property of local skin, sensitization and absorption test to animal.
Result: complete and damaged skin is without irritating property to Cavia porcellus in the present invention who makes medicated powder and make 2%, 5% ointment, and also nontoxic and sensitization, can not be absorbed and be caused and be poisoned to death by complete and damaged skin.
3, mucomembranous irritant test
By making the antiinflammatory inhibiting-bacteria preparation of the present invention of medicated powder, be prepared into high, medium and low group of concentration toothpaste, and rabbit and doggie are carried out to oral mucosa, gingival mucosa stimulation test.
Result: antiinflammatory inhibiting-bacteria preparation of the present invention to mucosa without irritating, also without pathologic lesion.
In sum, antiinflammatory inhibiting-bacteria preparation of the present invention is efficient, wide spectrum, has good fungistatic effect; Low drug resistance; Strengthen skin, mucosa resistance and nutrition skin; And safety coefficient is high, harmless, nontoxic, have no side effect, non-stimulated.
Correspondingly, as shown in Figure 1, the invention also discloses a kind of preparation method of antiinflammatory inhibiting-bacteria preparation, comprising:
S101, smashs Ilex rotunda Thunb. bark to pieces, obtains Ilex rotunda Thunb. bark pieces;
S102, by Ilex rotunda Thunb. bark pieces described in soak with ethanol, and carries out reflux, extract;
Preferably, in S102, the concentration of ethanol is 70 ~ 85%.
More preferably, in S102, the concentration of ethanol is 78 ~ 80%.
S103, the Ilex rotunda Thunb. active component concentrated extract by described reflux, extract,, obtains head product;
After reflux, extract, Ilex rotunda Thunb. active component, before concentrated extract, also need to reclaim ethanol, discard medicinal residues.
S104, by described head product washing, filters;
Preferably, in S104, described head product adds 2 ~ 4 times of water washings.
More preferably, in S104, described head product adds 3 times of water washings.
S105, carries out dried by the described head product through washing filtering, obtains final products antiinflammatory inhibiting-bacteria preparation.
Described antiinflammatory inhibiting-bacteria preparation, it mainly contains following active component:
Total ketone 0.1 ~ 5g/100g
Total Saponin 15 ~ 40g/100g
Triterpene 45 ~ 80mg/100g.
Preferably, described antiinflammatory inhibiting-bacteria preparation mainly contains following active component:
Total ketone 2 ~ 3g/100g
Total Saponin 25 ~ 32g/100g
Triterpene 60 ~ 68mg/100g.
More preferably, described antiinflammatory inhibiting-bacteria preparation mainly contains following active component:
Total ketone 2.43g/100g
Total Saponin 28.3g/100g
Triterpene 62.2mg/100g.
Preferably, described total ketone is total flavones.
With specific embodiment, further set forth the preparation method of antiinflammatory inhibiting-bacteria preparation of the present invention below
Embodiment I
Ilex rotunda Thunb. bark is smashed to pieces, obtained Ilex rotunda Thunb. bark pieces; By concentration, be 78% soak with ethanol Ilex rotunda Thunb. bark pieces, and carry out reflux, extract; Reclaim ethanol, discard medicinal residues, the Ilex rotunda Thunb. active component concentrated extract by reflux, extract,, obtains head product; Head product is added to 2 times of water washings, filter; Head product is carried out to dried, and obtaining active component is the final products antiinflammatory inhibiting-bacteria preparation of the total ketone of 1.58g/100g, the total Saponin of 16.4g/100g, 45mg/100g triterpene.
Embodiment II
Ilex rotunda Thunb. bark is smashed to pieces, obtained Ilex rotunda Thunb. bark pieces; By concentration, be 80% soak with ethanol Ilex rotunda Thunb. bark pieces, and carry out reflux, extract; Reclaim ethanol, discard medicinal residues, the Ilex rotunda Thunb. active component concentrated extract by reflux, extract,, obtains head product; Head product is added to 3 times of water washings, filter; Head product is carried out to dried, and obtaining active component is the final products antiinflammatory inhibiting-bacteria preparation of the total ketone of 2.43g/100g, the total Saponin of 28.3g/100g, 62.2mg/100g triterpene.
Embodiment III
Ilex rotunda Thunb. bark is smashed to pieces, obtained Ilex rotunda Thunb. bark pieces; By concentration, be 80% soak with ethanol Ilex rotunda Thunb. bark pieces, and carry out reflux, extract; Reclaim ethanol, discard medicinal residues, the Ilex rotunda Thunb. active component concentrated extract by reflux, extract,, obtains head product; Head product is added to 4 times of water washings, filter; Head product is carried out to dried, and obtaining active component is the final products antiinflammatory inhibiting-bacteria preparation of the total ketone of 5g/100g, the total Saponin of 40g/100g, 78mg/100g triterpene.
It should be noted that, in the preparation method of antiinflammatory inhibiting-bacteria preparation of the present invention, the process conditions such as reflux, extract,, concentrated extract, washing, dried are same as the prior art.
Correspondingly, the present invention also provides the application of above-mentioned antiinflammatory inhibiting-bacteria preparation, especially at toothpaste, and mouth cleaner, collutory oral health care product, individual's conditioning liquid for cleaning, the antibacterial lubricating fluid of vagina, the application of soap sheet, itch-stopping dew, bath gel, shampoo and beneficial skin frost daily chemical products.
(1), the application of antiinflammatory inhibiting-bacteria preparation of the present invention in toothpaste
Gingivitis, periodontitis, dental caries etc., majority is caused by antibacterial.In general, have two kinds of microorganisms in human oral cavity, a kind of is antibacterial, has aerobe and anaerobe, take anaerobe as leading, accounting for 90%, and another kind is protozoon, has gingivalis Endamoeba protozoon and buccalis, take gingivalis Endamoeba as main, accounts for 50%.These two kinds of microorganisms exist simultaneously, because oxygen concentration in the identical gums of living environment environmental condition is low, are conducive to both existence, the normal while of anaerobe and gingivalis Endamoeba protozoon also deposits, be conducive to both existence, be also conducive to both breedings, then cause oral disease.
1, the application of antiinflammatory inhibiting-bacteria preparation of the present invention in toothpaste: add antiinflammatory inhibiting-bacteria preparation active component of the present invention in toothpaste, its formula and preparation technology are mainly as follows:
Weight percent meter, in toothpaste, the addition of antiinflammatory inhibiting-bacteria preparation of the present invention is 0.5 ~ 2.0%, all the other adjuvants are for making the conventional adjuvant of toothpaste.
Preferably, by weight percentage, antiinflammatory inhibiting-bacteria preparation of the present invention accounts for 0.8 ~ 1% to formula consumption, abrasivus 18 ~ 40%, wetting agent 18 ~ 40%, binding agent 0.8 ~ 2%, foaming agent 0.6 ~ 2.5%, correctives 0.05 ~ 0.35%, essence 1 ~ 2%, deionized water 20 ~ 35%.The conventional adjuvant component sum of antiinflammatory inhibiting-bacteria preparation of the present invention and toothpaste is 100%.
As one of embodiment, optional use is filled a prescription below: antiinflammatory inhibiting-bacteria preparation of the present invention accounts for 2%, abrasivus 35%, wetting agent 35%, binding agent 1%, foaming agent 1%, correctives 0.2%, essence 1.8%, deionized water 24%.
Antiinflammatory inhibiting-bacteria preparation of the present invention is processed into powdery standby, add wetting agent and deionized water and stirring, make deionized water suspension, mix with abrasivus, binding agent, foaming agent, correctives again, stir, grind and vacuum outgas, finally add essence to stir, grind and vacuum outgas, obtain lotion of toothpaste.
Wherein, the wetting agent that toothpaste adds is sorbitol, glycerol, propylene glycol, xylitol or Polyethylene Glycol (400-600).Preferably, wetting agent is glycerol, xylitol, Polyethylene Glycol.More preferably, wetting agent is xylitol.
The binding agent that toothpaste adds is sodium alginate, carboxymethyl cellulose, xanthan gum, hydroxyethyl-cellulose, guar gum, carrageenan.Preferably, binding agent is sodium alginate, carboxymethyl cellulose, hydroxyethyl-cellulose.More preferably, binding agent is carboxymethyl cellulose.
The correctives that toothpaste adds is sucrose, glucide, cyclamate, dipeptide sodium.Preferably, correctives is sucrose, glucide.More preferably, correctives is cyclamate.
The essence that toothpaste adds is Oleum menthae, lemon sesame oil, Oleum Menthae Rotundifoliae, orange oil, citrus seed oil, winter wood oil, Oleum Caryophylli, Oleum Cinnamomi, mattress Oleum sesami, Borneolum Syntheticum.Preferably, essence is Oleum menthae, lemon sesame oil, Oleum Menthae Rotundifoliae, Borneolum Syntheticum.More preferably, essence is Oleum Menthae Rotundifoliae, Oleum menthae.
The foaming agent that toothpaste adds is sodium lauryl sulphate, N-sodium lauroyl sarcosine, methyl cocoa ester group sodium taurocholate, phenolic group sodium glutamate.Preferably, foaming agent is sodium lauryl sulphate, N-sodium lauroyl sarcosine, methyl cocoa ester group sodium taurocholate.More preferably, foaming agent is sodium lauryl sulphate.
2, ameba experimental result:
In conjunction with the ameba experiment schematic diagram of the toothpaste of the present invention shown in Fig. 2 A, Fig. 2 B, Fig. 2 C, Fig. 2 D, result shows: the toothpaste that contains the total ketone of antiinflammatory inhibiting-bacteria preparation active component of the present invention, total Saponin, triterpene has obvious inhibition to antibacterial and protozoon.
As shown in Figure 2 A, splash into before the toothpaste of application antiinflammatory inhibiting-bacteria preparation active component of the present invention, polypide is movable vivaciously, pseudopodium stretches, form is variable.
As shown in Figure 2 B, splash into after the toothpaste of application antiinflammatory inhibiting-bacteria preparation active component of the present invention after 1 ~ 3 second, that pseudopodium shrinks is motionless, become circular edge there is gauffer.
As shown in Figure 2 C, splash into application antiinflammatory inhibiting-bacteria preparation active component of the present invention toothpaste after after 10 seconds, Yi Bian polypide starts to split, after 22 seconds, endoparticle blackening is a plurality of splits.
As shown in Figure 2 D, splash into application antiinflammatory inhibiting-bacteria preparation active component of the present invention toothpaste after after 30 seconds, polypide is all split, blackening, profile is uneven.
3, antiinflammatory fungistatic effect test
Strains tested: staphylococcus aureus, staphylococcus epidermidis, Streptococcus viridans, Streptococcus mutans; Above-mentioned bacterial strains is test strain, without specificity requirement, and can obtain by conventional method separation.
Adopt the bacteriostatic activity of the above-mentioned toothpaste that contains the total ketone of antiinflammatory inhibiting-bacteria preparation active component of the present invention, total Saponin, triterpene of cave method or paper disk method test to above-mentioned strains tested, test result is as follows:
| Strains tested | Staphylococcus aureus | Staphylococcus epidermidis | Streptococcus viridans | Streptococcus mutans |
| Inhibition zone diameter (mm) | 22 | 21 | 23 | 20 |
Result: the toothpaste that contains the total ketone of antiinflammatory inhibiting-bacteria preparation active component of the present invention, total Saponin, triterpene all more than 20mm, has good fungistatic effect to above-mentioned bacterial strains inhibition zone diameter.
Therefore, apply the toothpaste of antiinflammatory inhibiting-bacteria preparation active component of the present invention, it contains total ketone, total Saponin, triterpene isoreactivity composition, has that antiinflammatory is antibacterial, parasite killing and a preventing caries function.
4, clinical observation:
The toothpaste of the total ketone of antiinflammatory inhibiting-bacteria preparation active component of the present invention, total Saponin, triterpene will be contained, in Nanfang Hospital's department of stomatology and center, oral cavity, West China, Shenzhen, 235 of gingivalis Endamoeba protozoan infection persons (135 of men, 100 of female) are established to matched group simultaneously, with double-blind method is random, use dissimilar toothpaste and other toothpaste of stopping using, check after four months, result is as follows:
The above results confirms: but the toothpaste of applying antiinflammatory inhibiting-bacteria preparation active component of the present invention in experimental group has certain effect of killing to oral cavity gingivalis Endamoeba protozoan infection person, and its effect is better than matched group.
(2), the application of antiinflammatory inhibiting-bacteria preparation of the present invention in mouth cleaner, collutory oral health care product
1, the application of antiinflammatory inhibiting-bacteria preparation of the present invention in mouth cleaner, collutory oral health care product: add 0.5 ~ 2.0% antiinflammatory inhibiting-bacteria preparation active component of the present invention in mouth cleaner, collutory, other adjuvants are the conventional adjuvant of preparing mouth cleaner, collutory, and adopt existing preparation method and condition, can obtain and contain the total ketone of antiinflammatory inhibiting-bacteria preparation active component of the present invention, total Saponin, the mouth cleaner of triterpene, collutory.
It should be noted that, the addition of described antiinflammatory inhibiting-bacteria preparation in mouth cleaner, collutory oral health care product is weight percentage 0.5 ~ 2.0%.
Preferably, the addition of described antiinflammatory inhibiting-bacteria preparation in mouth cleaner, collutory oral health care product is weight percentage 0.8 ~ 1.0%.
2, antiinflammatory fungistatic effect test
Strains tested: staphylococcus aureus, staphylococcus epidermidis, Streptococcus viridans, Streptococcus mutans; Above-mentioned bacterial strains is test strain, without specificity requirement, and can obtain by conventional method separation.
Adopt the above-mentioned total ketone of antiinflammatory inhibiting-bacteria preparation active component of the present invention, total Saponin, the mouth cleaner of triterpene, the bacteriostatic activity of collutory to above-mentioned strains tested of containing of paper disk method test, test result is as follows:
| Strains tested | Staphylococcus aureus | Staphylococcus epidermidis | Streptococcus viridans | Streptococcus mutans |
| Inhibition zone diameter (mm) | 15 | 16 | 11 | 13 |
Result: the mouth cleaner that contains the total ketone of antiinflammatory inhibiting-bacteria preparation active component of the present invention, total Saponin, triterpene, collutory all more than 10mm, have good fungistatic effect to above-mentioned bacterial strains inhibition zone diameter.
(3), the application of antiinflammatory inhibiting-bacteria preparation of the present invention in individual conditioning liquid for cleaning
1, the application of antiinflammatory inhibiting-bacteria preparation of the present invention in individual conditioning liquid for cleaning: add 0.5 ~ 2.0% antiinflammatory inhibiting-bacteria preparation active component of the present invention in individual conditioning liquid for cleaning, other adjuvants are the conventional adjuvant of the individual conditioning liquid for cleaning of preparation, and adopt existing preparation method and condition, can obtain the individual conditioning liquid for cleaning that contains the total ketone of antiinflammatory inhibiting-bacteria preparation active component of the present invention, total Saponin, triterpene.
It should be noted that, the addition of described antiinflammatory inhibiting-bacteria preparation in individual conditioning liquid for cleaning is weight percentage 0.5 ~ 2.0%.
Preferably, the addition of described antiinflammatory inhibiting-bacteria preparation in individual conditioning liquid for cleaning is weight percentage 0.8 ~ 1.0%.
2, antiinflammatory fungistatic effect test
Strains tested: staphylococcus aureus, staphylococcus epidermidis, Candida albicans; Above-mentioned bacterial strains is test strain, without specificity requirement, and can obtain by conventional method separation.
Adopt the bacteriostatic activity of the above-mentioned individual conditioning liquid for cleaning that contains the total ketone of antiinflammatory inhibiting-bacteria preparation active component of the present invention, total Saponin, triterpene of paper disk method test to above-mentioned strains tested, test result is as follows:
| Strains tested | Staphylococcus aureus | Staphylococcus epidermidis | ? Candida albicans |
| Inhibition zone diameter (mm) | 17 | 20 | 23 |
Result: the individual conditioning liquid for cleaning that contains the total ketone of antiinflammatory inhibiting-bacteria preparation active component of the present invention, total Saponin, triterpene all more than 10mm, has good fungistatic effect to above-mentioned bacterial strains inhibition zone diameter.
(4), the application of antiinflammatory inhibiting-bacteria preparation of the present invention in the antibacterial lubricating fluid of vagina
1, the application of antiinflammatory inhibiting-bacteria preparation of the present invention in the antibacterial lubricating fluid of vagina: add 0.5 ~ 2.0% antiinflammatory inhibiting-bacteria preparation active component of the present invention in the antibacterial lubricating fluid of vagina, other adjuvants are the conventional adjuvant of preparing the antibacterial lubricating fluid of vagina, and adopt existing preparation method and condition, can obtain the antibacterial lubricating fluid of vagina that contains the total ketone of antiinflammatory inhibiting-bacteria preparation active component of the present invention, total Saponin, triterpene.
It should be noted that, the addition of described antiinflammatory inhibiting-bacteria preparation in the antibacterial lubricating fluid of vagina is weight percentage 0.5 ~ 2.0%.
Preferably, the addition of described antiinflammatory inhibiting-bacteria preparation in the antibacterial lubricating fluid of vagina is weight percentage 0.8 ~ 1.0%.
2, antiinflammatory fungistatic effect test
Strains tested: staphylococcus aureus, staphylococcus epidermidis, Candida albicans; Above-mentioned bacterial strains is test strain, without specificity requirement, and can obtain by conventional method separation.
Adopt the bacteriostatic activity of the above-mentioned antibacterial lubricating fluid of vagina that contains the total ketone of antiinflammatory inhibiting-bacteria preparation active component of the present invention, total Saponin, triterpene of paper disk method test to above-mentioned strains tested, test result is as follows:
| Strains tested | Staphylococcus aureus | Staphylococcus epidermidis | ? Candida albicans |
| Inhibition zone diameter (mm) | 16 | 20 | 23 |
Result: the antibacterial lubricating fluid of vagina that contains the total ketone of antiinflammatory inhibiting-bacteria preparation active component of the present invention, total Saponin, triterpene all more than 10mm, has good fungistatic effect to above-mentioned bacterial strains inhibition zone diameter.
(5), the application of antiinflammatory inhibiting-bacteria preparation of the present invention in soap sheet
1, the application of antiinflammatory inhibiting-bacteria preparation of the present invention in soap sheet: add 0.5 ~ 2.0% antiinflammatory inhibiting-bacteria preparation active component of the present invention in soap sheet raw material, other adjuvants are the conventional adjuvant of preparing soap sheet, and adopt existing preparation method and condition, can obtain the soap sheet that contains the total ketone of antiinflammatory inhibiting-bacteria preparation active component of the present invention, total Saponin, triterpene.
It should be noted that, the addition of described antiinflammatory inhibiting-bacteria preparation in soap sheet is weight percentage 0.5 ~ 2.0%.
Preferably, the addition of described antiinflammatory inhibiting-bacteria preparation in soap sheet is weight percentage 0.8 ~ 1.0%.
2, antiinflammatory fungistatic effect
(1) experimenter as shown in Fig. 3 A, Fig. 3 B, Fig. 3 C, Fig. 3 D, Fig. 3 E, Fig. 3 F adopts the culture of bacteria of soap sheet of the present invention before and after washing one's hands to remove schematic diagram, above-mentioned six figure are that six experimenters adopt the soap sheet that contains the total ketone of antiinflammatory inhibiting-bacteria preparation active component of the present invention, total Saponin, triterpene, wash one's hands front and back with under same sampling condition, after palm of the hand specimen sampling, culture of bacteria is removed schematic diagram, each culture dish is an experimenter from left to right, represents up and down the contrast of clump count before and after washing one's hands.
Result: from Fig. 3 A to F, after washing one's hands with soap sheet of the present invention, clump count has obvious minimizing, and with soap sheet of the present invention, wash afterwards without residue paper scrap, meet environmental requirement.
(2) antiinflammatory fungistatic effect test: respectively experimenter is adopted respectively to bacterial colony count before and after washing one's hands of soap sheet of the present invention, common soap sheet and tap water and the mushroom statistics of doing experiment, specific as follows:
As seen from the above table, tap water group remains and has stayed 22.55% antibacterial; Common soap sheet group has retained 18.34% antibacterial; And soap sheet group of the present invention has only retained 1.18% antibacterial, and removed pathogenic mushroom.Although each group all has degerming effect in various degree, soap sheet group of the present invention is obviously better than other each group.
Result: the soap sheet that contains the total ketone of antiinflammatory inhibiting-bacteria preparation active component of the present invention, total Saponin, triterpene, has antibacterial, bacteria-eliminating efficacy preferably.
(6), the application of antiinflammatory inhibiting-bacteria preparation of the present invention in itch-stopping dew
1, the application of antiinflammatory inhibiting-bacteria preparation of the present invention in itch-stopping dew: add 0.5 ~ 2.0% antiinflammatory inhibiting-bacteria preparation active component of the present invention in itch-stopping dew raw material, other adjuvants are the conventional adjuvant of preparing itch-stopping dew, and adopt existing preparation method and condition, can obtain the itch-stopping dew that contains the total ketone of antiinflammatory inhibiting-bacteria preparation active component of the present invention, total Saponin, triterpene.
It should be noted that, the addition of described antiinflammatory inhibiting-bacteria preparation in itch-stopping dew is weight percentage 0.5 ~ 2.0%.
Preferably, the addition of described antiinflammatory inhibiting-bacteria preparation in itch-stopping dew is weight percentage 0.8 ~ 1.0%.
2, antiinflammatory fungistatic effect
(1) the antipruritic effect block diagram that as shown in Figure 4, Fig. 4 is itch-stopping dew of the present invention.Fig. 4 has shown the statistical result of using the antipruritic effect that contains itch-stopping dew of the present invention for 216 crowds of random selection, through observing proved invention itch-stopping dew, skin is crossed to skin, skin pruritus, eczema, miliaria, have obvious itching-relieving efficacies with sting etc., total effective rate reaches more than 95%.
Result: the itch-stopping dew that contains the total ketone of antiinflammatory inhibiting-bacteria preparation active component of the present invention, total Saponin, triterpene has good dispelling wind for relieving itching, the antibacterial effect of antiinflammatory.
(2) antiinflammatory fungistatic effect test
Strains tested: staphylococcus aureus, staphylococcus epidermidis, Candida albicans; Above-mentioned bacterial strains is test strain, without specificity requirement, and can obtain by conventional method separation.
Adopt the bacteriostatic activity of the above-mentioned itch-stopping dew that contains the total ketone of antiinflammatory inhibiting-bacteria preparation active component of the present invention, total Saponin, triterpene of paper disk method test to above-mentioned strains tested, test result is as follows:
| Strains tested | Staphylococcus aureus | Staphylococcus epidermidis | ? Candida albicans |
| Inhibition zone diameter (mm) | 11 | 10 | 11 |
Result: the itch-stopping dew that contains the total ketone of antiinflammatory inhibiting-bacteria preparation active component of the present invention, total Saponin, triterpene all more than 10mm, has good fungistatic effect to above-mentioned bacterial strains inhibition zone diameter.
(7), the application of antiinflammatory inhibiting-bacteria preparation of the present invention in bath gel
1, the application of antiinflammatory inhibiting-bacteria preparation of the present invention in bath gel: add 0.5 ~ 2.0% antiinflammatory inhibiting-bacteria preparation active component of the present invention in bath gel, other adjuvants are the conventional adjuvant of preparing bath gel, and adopt existing preparation method and condition, can obtain the bath gel that contains the total ketone of antiinflammatory inhibiting-bacteria preparation active component of the present invention, total Saponin, triterpene.
It should be noted that, the addition of described antiinflammatory inhibiting-bacteria preparation in bath gel is weight percentage 0.5 ~ 2.0%.
Preferably, the addition of described antiinflammatory inhibiting-bacteria preparation in bath gel is weight percentage 0.8 ~ 1.0%.
2, antiinflammatory fungistatic effect test
Strains tested: staphylococcus aureus, staphylococcus epidermidis, Candida albicans, Streptococcus viridans; Above-mentioned bacterial strains is test strain, without specificity requirement, and can obtain by conventional method separation.
Adopt the bacteriostatic activity of the above-mentioned bath gel that contains the total ketone of antiinflammatory inhibiting-bacteria preparation active component of the present invention, total Saponin, triterpene of paper disk method test to above-mentioned strains tested, test result is as follows:
| Strains tested | Staphylococcus aureus | Staphylococcus epidermidis | ? Candida albicans | Streptococcus viridans |
| Inhibition zone diameter (mm) | 21 | 16 | 25 | 18 |
Result: the bath gel that contains the total ketone of antiinflammatory inhibiting-bacteria preparation active component of the present invention, total Saponin, triterpene all more than 10mm, has good fungistatic effect to above-mentioned bacterial strains inhibition zone diameter.
(8), the application of antiinflammatory inhibiting-bacteria preparation of the present invention in shampoo
1, the application of antiinflammatory inhibiting-bacteria preparation of the present invention in shampoo: add 0.5 ~ 2.0% antiinflammatory inhibiting-bacteria preparation active component of the present invention in shampoo, other adjuvants are the conventional adjuvant of preparing shampoo, and adopt existing preparation method and condition, can obtain the shampoo that contains the total ketone of antiinflammatory inhibiting-bacteria preparation active component of the present invention, total Saponin, triterpene.
It should be noted that, the addition of described antiinflammatory inhibiting-bacteria preparation in shampoo is weight percentage 0.5 ~ 2.0%.
Preferably, the addition of described antiinflammatory inhibiting-bacteria preparation in shampoo is weight percentage 0.8 ~ 1.0%.
2, antiinflammatory fungistatic effect test
Strains tested: staphylococcus aureus, staphylococcus epidermidis, Candida albicans, Streptococcus viridans; Above-mentioned bacterial strains is test strain, without specificity requirement, and can obtain by conventional method separation.
Adopt the bacteriostatic activity of the above-mentioned shampoo that contains the total ketone of antiinflammatory inhibiting-bacteria preparation active component of the present invention, total Saponin, triterpene of paper disk method test to above-mentioned strains tested, test result is as follows:
| Strains tested | Staphylococcus aureus | Staphylococcus epidermidis | ? Candida albicans | Streptococcus viridans |
| Inhibition zone diameter (mm) | 17 | 18 | 22 | 18 |
Result: the shampoo that contains the total ketone of antiinflammatory inhibiting-bacteria preparation active component of the present invention, total Saponin, triterpene all more than 10mm, has good fungistatic effect to above-mentioned bacterial strains inhibition zone diameter.
(9), the application of antiinflammatory inhibiting-bacteria preparation of the present invention in beneficial skin frost
1, the application of antiinflammatory inhibiting-bacteria preparation of the present invention in beneficial skin frost: add 0.5 ~ 2.0% antiinflammatory inhibiting-bacteria preparation active component of the present invention in beneficial skin frost, other adjuvants are the conventional adjuvant of the beneficial skin frost of preparation, and adopt existing preparation method and condition, can obtain the beneficial skin frost that contains the total ketone of antiinflammatory inhibiting-bacteria preparation active component of the present invention, total Saponin, triterpene.
It should be noted that, the addition of described antiinflammatory inhibiting-bacteria preparation in beneficial skin frost is weight percentage 0.5 ~ 2.0%.
Preferably, the addition of described antiinflammatory inhibiting-bacteria preparation in beneficial skin frost is weight percentage 0.8 ~ 1.0%.
2, antiinflammatory fungistatic effect test
Strains tested: staphylococcus aureus, staphylococcus epidermidis, Candida albicans; Above-mentioned bacterial strains is test strain, without specificity requirement, and can obtain by conventional method separation.
Employing is the bacteriostatic activity to above-mentioned strains tested with the above-mentioned beneficial skin frost that contains the total ketone of antiinflammatory inhibiting-bacteria preparation active component of the present invention, total Saponin, triterpene of cave method test, and test result is as follows:
| Strains tested | Staphylococcus aureus | Staphylococcus epidermidis | ? Candida albicans |
| Inhibition zone diameter (mm) | 30 | 47 | 32 |
Result: the beneficial skin frost that contains the total ketone of antiinflammatory inhibiting-bacteria preparation active component of the present invention, total Saponin, triterpene all more than 10mm, has good fungistatic effect to above-mentioned bacterial strains inhibition zone diameter.
In sum, the present invention is applied to the series of products of daily life by the antiinflammatory inhibiting-bacteria preparation that contains total ketone, total Saponin, triterpene isoreactivity composition, comprising: toothpaste; The oral health care product such as mouth cleaner, collutory; Individual's conditioning liquid for cleaning, the antibacterial lubricating fluid of vagina; The daily chemical products such as soap sheet, itch-stopping dew, bath gel, shampoo and beneficial skin frost.Because antiinflammatory inhibiting-bacteria preparation has efficiently, wide spectrum, low drug resistance, medicine source is abundant and safety coefficient high.So, described in contain antiinflammatory inhibiting-bacteria preparation articles for use safe and reliable, antiinflammatory fungistatic effect is obvious.
With specific embodiment, to antiinflammatory inhibiting-bacteria preparation of the present invention, should be used as further explaination below
Embodiment 1: the preparation of toothpaste of the present invention
Ilex rotunda Thunb. bark is smashed to pieces, by concentration, be 80% soak with ethanol Ilex rotunda Thunb. bark pieces, and carry out reflux, extract,, concentrated extract, 3 times of water washings, filter, dry, are processed into powdery standby---antiinflammatory inhibiting-bacteria preparation of the present invention.
Take antiinflammatory inhibiting-bacteria preparation 200g of the present invention, add glycerol 3500g, add again deionized water 2000ml to stir into suspension, add calcium hydrogen phosphate 3500g, carboxymethyl cellulose 180g, sodium lauryl sulphate 250g, cyclamate 20g, stirring, grinding, vacuum outgas, finally add Oleum Menthae Rotundifoliae 150g agitation grinding, vacuum outgas, obtains lotion of toothpaste of the present invention.
Embodiment 2: the preparation of mouth cleaner of the present invention
Ilex rotunda Thunb. bark is smashed to pieces, by concentration, be 80% soak with ethanol Ilex rotunda Thunb. bark pieces, and carry out reflux, extract,, concentrated extract, 3 times of water washings, filter, dry, are processed into powdery standby---antiinflammatory inhibiting-bacteria preparation of the present invention.
Glycerol 1000g, polyoxyethylene hydrogenated Oleum Ricini 200g are joined in deionized water 8090g and dissolved, add again the essence solution being dissolved in ethanol, wherein essence content is 80g, ethanol 500g, after mix homogeneously, add antiinflammatory inhibiting-bacteria preparation 100g of the present invention, saccharin sodium 15g, sodium benzoate 5g, sodium dihydrogen phosphate 10g, dissolution filter can mouth cleaner of the present invention again.
Embodiment 3: the preparation of the present invention individual conditioning liquid for cleaning
Ilex rotunda Thunb. bark is smashed to pieces, by concentration, be 80% soak with ethanol Ilex rotunda Thunb. bark pieces, and carry out reflux, extract,, concentrated extract, 3 times of water washings, filter, dry, are processed into powdery standby---antiinflammatory inhibiting-bacteria preparation of the present invention.
Add 12 carbon fatty alcohol polyoxyethylene ether 18g, dodecyldimethylammonium hydroxide inner salt 18g and pelopon A 8g, propylene glycol 16g; lactic acid 3.2g; sodium hydroxide 0.8g; dodecyl dimethyl benzyl ammonium bromide 0.5g; antiinflammatory inhibiting-bacteria preparation 10g of the present invention; then add pure water to 900g left and right, open and stir, and heat up;
Be cooled to 30 ℃, add vitamin A 3g, continue to stir 2 minutes, with above-mentioned bronsted lowry acids and bases bronsted lowry, regulate pH value to 4.5, and add pure water to 1000g, stir and within 5 minutes, obtain the present invention individual conditioning liquid for cleaning.
Embodiment 4: the preparation of the antibacterial lubricating fluid of vagina of the present invention
Ilex rotunda Thunb. bark is smashed to pieces, by concentration, be 80% soak with ethanol Ilex rotunda Thunb. bark pieces, and carry out reflux, extract,, concentrated extract, 3 times of water washings, filter, dry, are processed into powdery standby---antiinflammatory inhibiting-bacteria preparation of the present invention.
Take vitamin E 0.6 g, vitamin A 0.09 g, vitamin D2 0 g, get liquid paraffin 100 g, said vitamin is added in liquid paraffin to lucifuge, with electric stirring, oil solution is stirred up and down, until evenly, separately get Mel 5g, be dissolved in sodium chloride 0.9% solution 6 g, add again 1% acetum 3 g, and antiinflammatory inhibiting-bacteria preparation 10g of the present invention, jointly stir, adjusting this liquid pH value is 4.0 ~ 4.5, packing, then, with flowing steam sterilization 30 minutes, obtains the antibacterial lubricating fluid of vagina of the present invention.
Embodiment 5: the preparation of soap sheet of the present invention
Ilex rotunda Thunb. bark is smashed to pieces, by concentration, be 80% soak with ethanol Ilex rotunda Thunb. bark pieces, and carry out reflux, extract,, concentrated extract, 3 times of water washings, filter, dry, are processed into powdery standby---antiinflammatory inhibiting-bacteria preparation of the present invention.
Get following raw material: soap base (antiinflammatory inhibiting-bacteria preparation/sulfur soap 3:97 of the present invention) 8g, stearic acid 2g, fatty acid distribution of coconut oil 8g, antiinflammatory inhibiting-bacteria preparation 2g of the present invention, octyl group glycerin ether sodium sulfonate 62.5g, ethylene hydroxy sulfuric acid sodium 5g, sodium chloride 4.5g, white lead 0. 5g that admires, orange bergamot essence 2g, distilled water 5. 5g;
Above-mentioned raw materials is mixed, be heated to molten condition mix homogeneously; After raw material spraying is dry, obtain soap powder; Essence is added in soap powder, after spice, grind evenly; Printing shaping after vacuum press strip, coolingly obtain soap sheet of the present invention.
Embodiment 6: the preparation of itch-stopping dew of the present invention
Ilex rotunda Thunb. bark is smashed to pieces, by concentration, be 80% soak with ethanol Ilex rotunda Thunb. bark pieces, and carry out reflux, extract,, concentrated extract, 3 times of water washings, filter, dry, are processed into powdery standby---antiinflammatory inhibiting-bacteria preparation of the present invention.
Get 75% ethanol 9355g and add in rustless steel container, add antiinflammatory inhibiting-bacteria preparation 100g of the present invention, stir it is dissolved completely; In whipping process, add according to this various essence, orange blossom oil 300 g for example, Fructus Citri Limoniae oil 60 g, oleum bergamottae 120 g, geranium absolute 25 g, benzoic acid 40 g, and mix homogeneously can obtain itch-stopping dew of the present invention.
Embodiment 7: the preparation of bath gel of the present invention
Ilex rotunda Thunb. bark is smashed to pieces, by concentration, be 80% soak with ethanol Ilex rotunda Thunb. bark pieces, and carry out reflux, extract,, concentrated extract, 3 times of water washings, filter, dry, are processed into powdery standby---antiinflammatory inhibiting-bacteria preparation of the present invention.
By antiinflammatory inhibiting-bacteria preparation 200g of the present invention, fatty alcohol polyoxyethylene ether sulfate 1800g, disodium alkyl polyoxyethylene sulfosuccinate 800g, coconut oil aminocarbonyl propyl betanin 1000g, lauryl alcohol diethyl phthalein amine 400g mix homogeneously in reactor, and be heated to 75 ℃; Deionized water 5330 is heated to, after 75 ℃, join in reactor, after stirring 5min, the glycerol 400g that is heated to 75 ℃ is added; Stirring cools to 50 ℃ and adds citric acid 30g, Rhizoma Curcumae Longae 20g, Fructus Citri Limoniae essence 10g, methyl hydroxybenzoate 10g, continues to stir to be cooled to room temperature and to obtain bath gel of the present invention.
Embodiment 8: the preparation of shampoo of the present invention
Ilex rotunda Thunb. bark is smashed to pieces, by concentration, be 80% soak with ethanol Ilex rotunda Thunb. bark pieces, and carry out reflux, extract,, concentrated extract, 3 times of water washings, filter, dry, are processed into powdery standby---antiinflammatory inhibiting-bacteria preparation of the present invention.
In reactor, add deionized water 5950g, be warming up to 75 ℃.Stir on one side, Yi Bian add successively fatty alcohol polyoxyethylene ether sulfate 2000g, cocoanut fatty acid diethanolamide 400g, cocamido propyl betaine 700g, oleoyl aminoacid sodium 500g, stir speed (S.S.) is 40 revs/min.After uniform dissolution, add again emulsified silicone oil 200g, climbazole 50g, zinc pyrithione 20g, after all dissolving, be cooled to gradually 45 ℃, add antiinflammatory inhibiting-bacteria preparation 100g of the present invention, vitamin B5 50g, dispersed after, add citric acid 10g, antiseptic 10g and essence 10g, continue to be stirred to evenly, obtain shampoo of the present invention.
Embodiment 9: the preparation of the present invention's benefit skin frost
Ilex rotunda Thunb. bark is smashed to pieces, by concentration, be 80% soak with ethanol Ilex rotunda Thunb. bark pieces, and carry out reflux, extract,, concentrated extract, 3 times of water washings, filter, dry, are processed into powdery standby---antiinflammatory inhibiting-bacteria preparation of the present invention.
Take raw material components below:
A component: spermol 30g, stearyl alcohol 40g, butyl stearate 80g, glycerol 50g, aliphatic alcohol polyethenoxy (25) ether 30g, lanoline 40g, D-panthenol 10g, antiinflammatory inhibiting-bacteria preparation 10g of the present invention;
B component: vitamin A 1g, vitamin E 2g, natural essence 2g, sodium benzoate 3g, methyl hydroxybenzoate 2g, distilled water 700g.
A component is slowly heated to 80 ℃ in jacketed pan, makes it miscible; Under constantly stirring, the sodium benzoate in B component, methyl hydroxybenzoate and distilled water are evenly added in jacketed pan and are uniformly mixed, after making it to dissolve evenly, be cooled to 40 ℃; And then respectively the VitAVitE in B component and natural essence are added in jacketed pan and be uniformly mixed, after homogenizing, obtain can dividing and being filled in Packaging Bottle after mastic, obtain the present invention's benefit skin frost.
In sum, implement the present invention and there is following beneficial effect:
One, antiinflammatory inhibiting-bacteria preparation of the present invention is the active component extracting from holly plant Ilex rotunda Thunb. (having another name called Cortex Ilicis Rotundae), and active component is the total ketone of 0.1 ~ 5g/100g, the total Saponin of 15 ~ 40g/100g, 45 ~ 80mg/100g triterpene.Above-mentioned active component has heat-clearing and toxic substances removing, the antibacterial effect of antiinflammatory.Therefore, antiinflammatory inhibiting-bacteria preparation of the present invention have efficiently, wide spectrum, low drug resistance and safety coefficient high.
Two, the preparation method of antiinflammatory inhibiting-bacteria preparation of the present invention is mainly by the effective active composition in reflux, extract, natural plants Ilex rotunda Thunb., and its medicine source is abundant, simple to operate, with low cost, applied range.
Three, the present invention is applied to the series of products of daily life by the antiinflammatory inhibiting-bacteria preparation that contains total ketone, total Saponin, triterpene isoreactivity composition, comprising: toothpaste; The oral health care product such as mouth cleaner, collutory; Individual's conditioning liquid for cleaning, the antibacterial lubricating fluid of vagina; The daily chemical products such as soap sheet, itch-stopping dew, bath gel, shampoo and beneficial skin frost.And antiinflammatory inhibiting-bacteria preparation be take addition and is made an addition in above-mentioned articles for use as 0.5 ~ 2.0%.
Because antiinflammatory inhibiting-bacteria preparation has efficiently, wide spectrum, low drug resistance, medicine source is abundant and safety coefficient high.So, described in contain antiinflammatory inhibiting-bacteria preparation articles for use safe and reliable, antiinflammatory fungistatic effect is obvious.
The above is the preferred embodiment of the present invention; it should be pointed out that for those skilled in the art, under the premise without departing from the principles of the invention; can also make some improvements and modifications, these improvements and modifications are also considered as protection scope of the present invention.
Claims (8)
1. an antiinflammatory inhibiting-bacteria preparation, is characterized in that, it mainly contains following active component:
Total flavones 1.58 ~ 5g/100g
Total Saponin 16.4 ~ 40g/100g
Triterpene 45 ~ 78mg/100g;
The main active of described antiinflammatory inhibiting-bacteria preparation is extracted and is obtained from Ilex rotunda Thunb.;
Described antiinflammatory inhibiting-bacteria preparation is prepared by following methods and is obtained:
Ilex rotunda Thunb. bark is smashed to pieces, obtained Ilex rotunda Thunb. bark pieces;
By Ilex rotunda Thunb. bark pieces described in soak with ethanol, and carry out reflux, extract;
Ilex rotunda Thunb. active component concentrated extract by described reflux, extract,, obtains head product;
By described head product washing, filter;
The described head product through washing filtering is carried out to dried, obtain final products antiinflammatory inhibiting-bacteria preparation;
Wherein, the concentration of ethanol is 78 ~ 80%;
Described head product adds 2 ~ 4 times of water washings.
2. antiinflammatory inhibiting-bacteria preparation as claimed in claim 1, is characterized in that, described antiinflammatory inhibiting-bacteria preparation mainly contains following active component:
Total flavones 2.43g/100g
Total Saponin 28.3g/100g
Triterpene 62.2mg/100g.
3. the preparation method of the antiinflammatory inhibiting-bacteria preparation described in claim 1 ~ 2 any one, is characterized in that, comprising:
Ilex rotunda Thunb. bark is smashed to pieces, obtained Ilex rotunda Thunb. bark pieces;
By Ilex rotunda Thunb. bark pieces described in soak with ethanol, and carry out reflux, extract;
Ilex rotunda Thunb. active component concentrated extract by described reflux, extract,, obtains head product;
By described head product washing, filter;
The described head product through washing filtering is carried out to dried, obtain final products antiinflammatory inhibiting-bacteria preparation;
Described antiinflammatory inhibiting-bacteria preparation, it mainly contains following active component:
Total flavones 1.58 ~ 5g/100g
Total Saponin 16.4 ~ 40g/100g
Triterpene 45 ~ 78mg/100g;
Wherein, the concentration of ethanol is 78 ~ 80%;
Described head product adds 2 ~ 4 times of water washings.
4. the application of the antiinflammatory inhibiting-bacteria preparation described in claim 1 ~ 2 any one in toothpaste.
5. the application of the antiinflammatory inhibiting-bacteria preparation described in claim 1 ~ 2 any one in mouth cleaner, collutory oral health care product.
6. the application of the antiinflammatory inhibiting-bacteria preparation described in claim 1 ~ 2 any one in individual conditioning liquid for cleaning, the antibacterial lubricating fluid of vagina.
7. the application of the antiinflammatory inhibiting-bacteria preparation described in claim 1 ~ 2 any one in soap sheet, itch-stopping dew, bath gel, shampoo and beneficial skin frost daily chemical products.
8. the application of the antiinflammatory inhibiting-bacteria preparation as described in claim 4 ~ 7 any one, is characterized in that, the addition of described antiinflammatory inhibiting-bacteria preparation in articles for use is 0.5 ~ 2.0%.
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| CN105362118B (en) * | 2015-07-14 | 2018-11-16 | 武汉华士特工业生物技术开发有限公司 | A kind of maca health-care toothpaste and preparation method |
| CN106420420A (en) * | 2016-10-28 | 2017-02-22 | 楚雄乌蛮渡农垦有限公司 | Elsholtzia rugulosa essential oil toothpaste and preparation method thereof |
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