CN103191159A - Inflammation-diminishing bacterium-inhibition preparation and its preparation method and use - Google Patents
Inflammation-diminishing bacterium-inhibition preparation and its preparation method and use Download PDFInfo
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Abstract
The invention discloses an inflammation-diminishing bacterium-inhibition preparation. The inflammation-diminishing bacterium-inhibition preparation contains active components of 0.1-5g/100g of total ketones, 15-40g/100g of total saponins, and 45-80g/100g of triterpene. The invention also discloses a preparation method of the inflammation-diminishing bacterium-inhibition preparation, and use of the inflammation-diminishing bacterium-inhibition preparation in oral health products such as toothpaste, Koujiesu and mouthwash, a personal cleaning care liquid, a vagina bacterium-inhibition lubricating liquid, a soap sheet, itching-relieving dew, body wash, shampoo and skin care cream. The inflammation-diminishing bacterium-inhibition preparation has the characteristics of high efficiency, wide spectrum, drug resistance, rich drug sources and high safety coefficient and has obvious effects of inflammation diminishing and bacterium inhibition.
Description
Technical field
The present invention relates to field of medicaments, particularly a kind of antiinflammatory inhibiting-bacteria preparation and its preparation method and application.
Background technology
Bacterial infection disease is the most common in clinical position, and using the antibiotic therapy bacterial infection disease has been one of the most general Therapeutic Method.In recent years, because human abuse for antibiotics, antibacterial presents fast rise trend to drug resistance, Drug resistance, the persister of each antibiotics always, even produces the situation that " powerful antibacterial " pasts medical help, and the toxic and side effects of medicine is also along with increase.
China abuses one of the most serious country of antibiotics in the world, and the bacterial drug resistance problem that causes thus is particularly outstanding.Some antibacterials of clinical separation are occupied first place in the world to the drug resistance of some drugs.For example, in the staphylococcus aureus that infect in Shanghai, 80% is own through having produced the drug resistance to benzylpenicillin.The resistant rate of quinolones antibiotics has reached 60% ~ 70%.Many antibiotics antibacterial efficacies commonly used day by day lower, and the antibiotics drug resistance has become the difficult problem that global public health faces.World Health Organization (WHO) (World Health Organization) claims that in the face of the antibiotics drug resistance problem that has increased rapidly since nearly half a century, medical circle is felt simply helpless.
In addition, a plurality of positions of human body such as skin, oral cavity, vagina etc. are owing to expose or partly be exposed to body surface, are subjected to antibacterial, other infection by microorganisms or biting of insecticide easily and cause disease or discomfort.But this situation is then unfavorable as using antibiotics, if it is safer to use some externals to have an antiinflammatory antibacterial medicines.
In the face of serious day by day bacterial drug resistance problem, reduce the generation of Resistant strain and develop the important topic that the high antibacterial-anti-inflammatory drug of new safety coefficient becomes the 21 century medical circle.Chinese medicine is the treasure-house of a greatness, and China working people has summed up the rich experiences of a whole set of disease preventing and treating for a long time in the practice process that combats the disease for thousands of years.Before modernized antibiotics was born, Chinese medicine was embodying its distinctive effect aspect some sterilizations, the antiinflammatory.Based on Chinese medical theory, what the Chinese medicine of most of clearing away heat-fires, clearing away heat and cooling blood, heat-clearing and toxic substances removing, cooling blood for hemostasis all had a varying degree kills (pressing down) bacterium activity, and has been widely used in clinically, and is obtaining some achievements aspect the bacterial infection treating.Because the particularity of Chinese medicine, antibacterial is less to produce drug resistance to Chinese medicine.
Therefore, that research has is efficient, wide spectrum, low drug resistance and the high Chinese medicine medicine of safety coefficient, has strong reality significance for the treatment bacterial inflammation.
Summary of the invention
Embodiment of the invention technical problem to be solved is, provides a kind of efficient, wide spectrum, low drug resistance, the medicine source is abundant and the high antiinflammatory inhibiting-bacteria preparation of safety coefficient.
Embodiment of the invention technical problem to be solved also is, provides a kind of efficient, wide spectrum, low drug resistance, the medicine source is abundant and the preparation method of the antiinflammatory inhibiting-bacteria preparation that safety coefficient is high.
Embodiment of the invention technical problem to be solved also is, the application of above-mentioned antiinflammatory inhibiting-bacteria preparation is provided, especially at toothpaste, mouth cleaner, collutory oral health care product, individual's conditioning liquid for cleaning, the antibacterial lubricating fluid of vagina, the application of soap sheet, itch-stopping dew, bath gel, shampoo and beneficial skin frost day product.
For reaching above-mentioned technique effect, the embodiment of the invention provides a kind of antiinflammatory inhibiting-bacteria preparation, and it mainly contains following active component:
Total ketone 0.1 ~ 5g/100g
Total Saponin 15 ~ 40g/100g
Triterpene 45 ~ 80mg/100g.
As the improvement of such scheme, described antiinflammatory inhibiting-bacteria preparation mainly contains following active component:
Total ketone 2.43g/100g
Total Saponin 28.3g/100g
Triterpene 62.2mg/100g.
As the improvement of such scheme, the main active of described antiinflammatory inhibiting-bacteria preparation is extracted from Ilex rotunda Thunb. and is got.
As the improvement of such scheme, described total ketone is total flavones.
Correspondingly, the invention also discloses a kind of preparation method of antiinflammatory inhibiting-bacteria preparation, comprising:
The Ilex rotunda Thunb. bark is smashed to pieces, obtained the Ilex rotunda Thunb. bark pieces;
With the described Ilex rotunda Thunb. bark pieces of soak with ethanol, and carry out reflux, extract;
Ilex rotunda Thunb. active component concentrated extract with described reflux, extract, obtains head product;
With described head product washing, filter;
Described head product through washing filtering is carried out dried, obtain final products antiinflammatory inhibiting-bacteria preparation;
Described antiinflammatory inhibiting-bacteria preparation, it mainly contains following active component:
Total ketone 0.1 ~ 5g/100g
Total Saponin 15 ~ 40g/100g
Triterpene 45 ~ 80mg/100g.
As the improvement of such scheme, described with the described Ilex rotunda Thunb. bark pieces of soak with ethanol, and carry out in the step of reflux, extract,, concentration of ethanol is 70 ~ 85%.
As the improvement of such scheme, described with described head product washing, in the step of filtration, described head product adds 2 ~ 4 times of water washings.
As the improvement of such scheme, described antiinflammatory inhibiting-bacteria preparation mainly contains following active component:
Total ketone 2.43g/100g
Total Saponin 28.3g/100g
Triterpene 62.2mg/100g.
As the improvement of such scheme, described total ketone is total flavones.
Correspondingly, the present invention also provides the application of above-mentioned antiinflammatory inhibiting-bacteria preparation in toothpaste, and the addition of described antiinflammatory inhibiting-bacteria preparation in toothpaste is 0.5 ~ 2.0%.
Need to prove that the addition of described antiinflammatory inhibiting-bacteria preparation in toothpaste is weight percentage 0.5 ~ 2.0%.
Correspondingly, the present invention also provides the application of above-mentioned antiinflammatory inhibiting-bacteria preparation in mouth cleaner, collutory oral health care product, and the addition of described antiinflammatory inhibiting-bacteria preparation in mouth cleaner, collutory oral health care product is 0.5 ~ 2.0%.
Need to prove that the addition of described antiinflammatory inhibiting-bacteria preparation in mouth cleaner, collutory oral health care product is weight percentage 0.5 ~ 2.0%.
Correspondingly, the present invention also provides the application of above-mentioned antiinflammatory inhibiting-bacteria preparation in individual conditioning liquid for cleaning, the antibacterial lubricating fluid of vagina, and the addition of described antiinflammatory inhibiting-bacteria preparation in individual conditioning liquid for cleaning, the antibacterial lubricating fluid of vagina is 0.5 ~ 2.0%.
Need to prove that the addition of described antiinflammatory inhibiting-bacteria preparation in individual conditioning liquid for cleaning, the antibacterial lubricating fluid of vagina is weight percentage 0.5 ~ 2.0%.
Correspondingly, the present invention also provides the application of above-mentioned antiinflammatory inhibiting-bacteria preparation in soap sheet, itch-stopping dew, bath gel, shampoo and beneficial skin frost day product, and the addition of described antiinflammatory inhibiting-bacteria preparation in soap sheet, itch-stopping dew, bath gel, shampoo and beneficial skin frost day product is 0.5 ~ 2.0%.
Need to prove that the addition of described antiinflammatory inhibiting-bacteria preparation in soap sheet, itch-stopping dew, bath gel, shampoo and beneficial skin frost day product is weight percentage 0.5 ~ 2.0%.
Implement the present invention and have following beneficial effect:
One, antiinflammatory inhibiting-bacteria preparation of the present invention is the active component that extracts from holly plant Ilex rotunda Thunb. (having another name called Cortex Ilicis Rotundae), and active component is the total ketone of 0.1 ~ 5g/100g, the total Saponin of 15 ~ 40g/100g, 45 ~ 80mg/100g triterpene.Above-mentioned active component has heat-clearing and toxic substances removing, the antibacterial effect of antiinflammatory.Therefore, antiinflammatory inhibiting-bacteria preparation of the present invention have efficiently, characteristics such as wide spectrum, low drug resistance and safety coefficient height.
Two, the preparation method of antiinflammatory inhibiting-bacteria preparation of the present invention mainly is by the effective active composition in the reflux, extract, natural plants Ilex rotunda Thunb., and its medicine source is abundant, and is simple to operate, with low cost, applied range.
Three, the present invention will contain total ketone, always the antiinflammatory inhibiting-bacteria preparation of Saponin, triterpene isoreactivity composition is applied to the series of products of daily life, comprise: toothpaste; Oral health care product such as mouth cleaner, collutory; Individual's conditioning liquid for cleaning, the antibacterial lubricating fluid of vagina; Daily chemical products such as soap sheet, itch-stopping dew, bath gel, shampoo and beneficial skin frost.And the antiinflammatory inhibiting-bacteria preparation is 0.5 ~ 2.0% to make an addition in the above-mentioned articles for use with addition.
Because the antiinflammatory inhibiting-bacteria preparation has efficiently, wide spectrum, low drug resistance, enrich in the medicine source and characteristics such as safety coefficient height.So the described articles for use that contain the antiinflammatory inhibiting-bacteria preparation are safe and reliable, the antiinflammatory fungistatic effect is obvious.
Description of drawings
Fig. 1 is the flow chart of the preparation method of a kind of antiinflammatory inhibiting-bacteria preparation of the present invention.
Fig. 2 A, Fig. 2 B, Fig. 2 C, Fig. 2 D test sketch map for the ameba of toothpaste of the present invention;
Fig. 3 A, Fig. 3 B, Fig. 3 C, Fig. 3 D, Fig. 3 E, Fig. 3 F for the experimenter adopts the culture of bacteria of soap sheet of the present invention before and after washing one's hands remove sketch map;
Fig. 4 is the antipruritic effect block diagram of itch-stopping dew of the present invention.
The specific embodiment
For making the purpose, technical solutions and advantages of the present invention clearer, below with the present invention is described in further detail.
The embodiment of the invention provides a kind of antiinflammatory inhibiting-bacteria preparation, and it mainly contains following active component:
Total ketone 0.1 ~ 5g/100g
Total Saponin 15 ~ 40g/100g
Triterpene 45 ~ 80mg/100g.
Preferably, described antiinflammatory inhibiting-bacteria preparation mainly contains following active component:
Total ketone 2 ~ 3g/100g
Total Saponin 25 ~ 32g/100g
Triterpene 60 ~ 68mg/100g.
More preferably, described antiinflammatory inhibiting-bacteria preparation mainly contains following active component:
Total ketone 2.43g/100g
Total Saponin 28.3g/100g
Triterpene 62.2mg/100g.
Above-mentioned total ketone is total flavones.
Need to prove, what antiinflammatory inhibiting-bacteria preparation of the present invention can be for medicated powder, ointment, spray, patch etc., but be not limited thereto.
Further, the main active of described antiinflammatory inhibiting-bacteria preparation is extracted from Ilex rotunda Thunb. and is got.
Ilex rotunda Thunb. (having another name called Cortex Ilicis Rotundae) is evergreen shrubs or arbor, 20 meters of Gao Keda, and the diameter of a cross-section of a tree trunk 1.3 meters above the ground reaches 1 meter; The bark Lycoperdon polymorphum Vitt is to grey black.Sprig is cylindrical, and is straight and upright, old branch tool longitudinal crevasse, and leaf scar obovate or triangle swell slightly, and hole skin is not obvious, gives birth to the vertical rib of sprout tool then, does not have hair, and is rare by little pubescence; The terminal bud taper shape.Be born in the hillside evergreen broadleaf forest of height above sea level 400-1100 rice and border.The happiness warm and humid climate.
The Ilex rotunda Thunb. medicinal part: bark or root bark with plant are used as medicine.
The Ilex rotunda Thunb. effect cures mainly: heat-clearing and toxic substances removing; Dampness removing; Pain relieving; Cold, fever; Tonsillitis; Laryngopharynx swelling and pain; Acute, chronic hepatitis; Acute gastroenteritis; Gastric and duodenal ulcers; Administration's diarrhea due to damp pathogen rushes down; Jaundice; Dysentery; Traumatic injury; Rheumatic arthralgia; Eczema; Furuncle.
The active component that antiinflammatory inhibiting-bacteria preparation of the present invention mainly extracts from the dry bark of holly plant Ilex rotunda Thunb. contains total ketone 0.1 ~ 5g/100g, total Saponin 15 ~ 40g/100g, triterpene 45 ~ 80mg/100g through China Guangzhou Analysis ﹠. Test Center's detection.Above-mentioned active component has heat-clearing and toxic substances removing, the antibacterial effect of antiinflammatory.
Below in conjunction with experimental data or detection data antiinflammatory inhibiting-bacteria preparation of the present invention is further elaborated.
One, the present invention is carried out the mensuration of antiinflammatory fungistatic effect, specific as follows:
1, the aerobe fungistatic effect is measured:
Use antiinflammatory inhibiting-bacteria preparation of the present invention through Nat'l Pharmaceutical ﹠ Biological Products Control Institute's probation redport (calibrating SJMS92591), bacterial strains such as the staphylococcus aureus that oneself is known, shigella flexneri, escherichia coli, dysentery bacterium, streptococcus faecalis are done the fungistatic effect test respectively, and result of the test is as follows:
| Strains tested | Staphylococcus aureus | Shigella flexneri | Escherichia coli | Dysentery bacterium | Streptococcus faecalis |
| Inhibition zone diameter (mm) | 10.5 | 10.0 | 10.2 | 10.3 | 10.5 |
Need to prove that above-mentioned bacterial strains is test strain, no specificity requirement, and can obtain by the conventional method separation.
The measurement result of minimum inhibitory concentration is as follows:
| Strains tested | Staphylococcus aureus | Shigella flexneri | Escherichia coli | Dysentery bacterium | Streptococcus faecalis |
| Minimum inhibitory concentration (mg/ml) | 50 | ﹥100 | 50 | ﹥100 | 50 |
The result: antiinflammatory inhibiting-bacteria preparation of the present invention, has fungistatic effect preferably, and has measured minimum inhibitory concentration all more than 10mm the above-mentioned bacterial strains inhibition zone diameter.
2, the anaerobe fungistatic effect is measured
Use antiinflammatory inhibiting-bacteria preparation of the present invention that the black bacteroid of bacillus perfringens, clostridium tetani, product of anaerobism, bacteroides fragilis, propionibacterium acnes and common Candida albicans etc. are done the fungistatic effect test through Nat'l Pharmaceutical ﹠ Biological Products Control Institute's probation redport (SJMS92579), result of the test is as follows:
| Strains tested | Bacillus perfringens | Clostridium tetani | Produce black bacteroid | Bacteroides fragilis | Propionibacterium acnes | Candida albicans |
| Inhibition zone diameter (mm) | 12.0 | 10.7 | 10.2 | 11.5 | 10.5 | 10.0 |
Need to prove that above-mentioned bacterial strains is test strain, no specificity requirement, and can obtain by the conventional method separation.
The measurement result of minimum inhibitory concentration is as follows:
| Strains tested | Bacillus perfringens | Clostridium tetani | Produce black bacteroid | Bacteroides fragilis | Propionibacterium acnes | Candida albicans |
| Minimum inhibitory concentration (mg/ml) | 25 | 25 | 25 | 100 | 100 | 50 |
The result: antiinflammatory inhibiting-bacteria preparation of the present invention, has fungistatic effect preferably, and has measured minimum inhibitory concentration all more than 10mm the above-mentioned bacterial strains inhibition zone diameter.
Two, nutrient is measured:
This product detects through China Guangzhou Analysis ﹠. Test Center and contains 16 kinds in aminoacid, be respectively aspartic acid, glutamic acid, serine, histidine, glycine, threonine, arginine, alanine, tyrosine, valine, methionine, phenylalanine, isoleucine, leucine, lysine, proline, total content 91.4mg/100g; Polysaccharide 2.67g/100ml.And contain vitamin B1, B2, C, K etc., be many with vitamin B1, B2 especially.
The result: antiinflammatory inhibiting-bacteria preparation of the present invention contains above-mentioned nutrient, has certain effect to strengthening skin, mucosa resistance and nutrition skin.
Three, security test:
Antiinflammatory inhibiting-bacteria preparation of the present invention has antiinflammatory fungistatic effect preferably after tested, but whether must will note safety in application process, therefore, has done following safety toxicity test, comprising: acute toxicity test, local skin test, mucosa stimulation test.
The result: above-mentioned toxicological test confirm antiinflammatory inhibiting-bacteria preparation of the present invention be a kind of harmless, nontoxic, have no side effect, non-stimulated big article of safety coefficient.
1, acute toxicity test
Antiinflammatory inhibiting-bacteria preparation of the present invention is used kunming mice body weight 18-20 gram ♂ ♀ half and half, experimentize with abdomen injection and administration by gavage.
The result:
A, intraperitoneal injection:
LD
50=2.022g/kg
Average fiducial limit=2.022 ± 0.1545k/kg
B, administration by gavage:
Use greater than the antiinflammatory inhibiting-bacteria preparation of the present invention of ten multiple doses and irritate stomach 2 times/day, the no abnormality seen reaction.
2, tuerculoderma
The present invention that use is made medicated powder and made 2%, 5% ointment carries out irritating property of local skin, sensitization and absorption test to animal.
The result: complete and damaged skin does not have the property of irritating to Cavia porcellus in the present invention who makes medicated powder and make 2%, 5% ointment, and also nontoxic and sensitization can not absorb by complete and damaged skin and cause and be poisoned to death.
3, mucomembranous irritant test
With making the antiinflammatory inhibiting-bacteria preparation of the present invention of medicated powder, be prepared into high, medium and low group of concentration toothpaste, and rabbit and doggie are carried out oral mucosa, gingival mucosa stimulation test.
The result: antiinflammatory inhibiting-bacteria preparation of the present invention does not have mucosa and irritates, and does not also have the pathologic infringement.
In sum, antiinflammatory inhibiting-bacteria preparation of the present invention is efficient, wide spectrum, and fungistatic effect is preferably arranged; Low drug resistance; Strengthen skin, mucosa resistance and nutrition skin; And the safety coefficient height, harmless, nontoxic, have no side effect, non-stimulated.
Correspondingly, as shown in Figure 1, the invention also discloses a kind of preparation method of antiinflammatory inhibiting-bacteria preparation, comprising:
S101 smashs the Ilex rotunda Thunb. bark to pieces, obtains the Ilex rotunda Thunb. bark pieces;
S102 with the described Ilex rotunda Thunb. bark pieces of soak with ethanol, and carries out reflux, extract;
Preferably, among the S102, concentration of ethanol is 70 ~ 85%.
More preferably, among the S102, concentration of ethanol is 78 ~ 80%.
S103, the Ilex rotunda Thunb. active component concentrated extract with described reflux, extract, obtains head product;
After reflux, extract, Ilex rotunda Thunb. active component, before the concentrated extract, also need reclaim ethanol, discard medicinal residues.
S104 with described head product washing, filters;
Preferably, among the S104, described head product adds 2 ~ 4 times of water washings.
More preferably, among the S104, described head product adds 3 times of water washings.
S105 carries out dried with described head product through washing filtering, obtains final products antiinflammatory inhibiting-bacteria preparation.
Described antiinflammatory inhibiting-bacteria preparation, it mainly contains following active component:
Total ketone 0.1 ~ 5g/100g
Total Saponin 15 ~ 40g/100g
Triterpene 45 ~ 80mg/100g.
Preferably, described antiinflammatory inhibiting-bacteria preparation mainly contains following active component:
Total ketone 2 ~ 3g/100g
Total Saponin 25 ~ 32g/100g
Triterpene 60 ~ 68mg/100g.
More preferably, described antiinflammatory inhibiting-bacteria preparation mainly contains following active component:
Total ketone 2.43g/100g
Total Saponin 28.3g/100g
Triterpene 62.2mg/100g.
Preferably, described total ketone is total flavones.
Further set forth the preparation method of antiinflammatory inhibiting-bacteria preparation of the present invention below with specific embodiment
The embodiment I
The Ilex rotunda Thunb. bark is smashed to pieces, obtained the Ilex rotunda Thunb. bark pieces; Be 78% soak with ethanol Ilex rotunda Thunb. bark pieces with concentration, and carry out reflux, extract; Reclaim ethanol, discard medicinal residues, the Ilex rotunda Thunb. active component concentrated extract with reflux, extract, obtains head product; Head product is added 2 times of water washings, filter; Head product is carried out dried, and obtaining active component is the final products antiinflammatory inhibiting-bacteria preparation of the total ketone of 1.58g/100g, the total Saponin of 16.4g/100g, 45mg/100g triterpene.
The embodiment II
The Ilex rotunda Thunb. bark is smashed to pieces, obtained the Ilex rotunda Thunb. bark pieces; Be 80% soak with ethanol Ilex rotunda Thunb. bark pieces with concentration, and carry out reflux, extract; Reclaim ethanol, discard medicinal residues, the Ilex rotunda Thunb. active component concentrated extract with reflux, extract, obtains head product; Head product is added 3 times of water washings, filter; Head product is carried out dried, and obtaining active component is the final products antiinflammatory inhibiting-bacteria preparation of the total ketone of 2.43g/100g, the total Saponin of 28.3g/100g, 62.2mg/100g triterpene.
The embodiment III
The Ilex rotunda Thunb. bark is smashed to pieces, obtained the Ilex rotunda Thunb. bark pieces; Be 80% soak with ethanol Ilex rotunda Thunb. bark pieces with concentration, and carry out reflux, extract; Reclaim ethanol, discard medicinal residues, the Ilex rotunda Thunb. active component concentrated extract with reflux, extract, obtains head product; Head product is added 4 times of water washings, filter; Head product is carried out dried, and obtaining active component is the final products antiinflammatory inhibiting-bacteria preparation of the total ketone of 5g/100g, the total Saponin of 40g/100g, 78mg/100g triterpene.
Need to prove that in the preparation method of antiinflammatory inhibiting-bacteria preparation of the present invention, process conditions such as reflux, extract,, concentrated extract, washing, dried are same as the prior art.
Correspondingly, the present invention also provides the application of above-mentioned antiinflammatory inhibiting-bacteria preparation, especially at toothpaste, and mouth cleaner, collutory oral health care product, individual's conditioning liquid for cleaning, the antibacterial lubricating fluid of vagina, the application of soap sheet, itch-stopping dew, bath gel, shampoo and beneficial skin frost day product.
(1), the application of antiinflammatory inhibiting-bacteria preparation of the present invention in toothpaste
Gingivitis, periodontitis, dental caries etc., majority is caused by antibacterial.In general, two kinds of microorganisms are arranged in the human oral cavity, a kind of is antibacterial, and aerobe and anaerobe are arranged, and based on anaerobe, account for 90%, another kind is protozoon, and gingivalis Endamoeba protozoon and buccalis are arranged, and based on gingivalis Endamoeba, accounts for 50%.These two kinds of microorganisms exist simultaneously, because oxygen concentration is low in the identical gums of living environment environmental condition, are conducive to both existence, the normal while of anaerobe and gingivalis Endamoeba protozoon also deposits, be conducive to both existence, also be conducive to both breedings, cause oral disease then.
1, the application of antiinflammatory inhibiting-bacteria preparation of the present invention in toothpaste: add antiinflammatory inhibiting-bacteria preparation active component of the present invention in toothpaste, its prescription and preparation technology are mainly as follows:
Weight percent meter, the addition of antiinflammatory inhibiting-bacteria preparation of the present invention is 0.5 ~ 2.0% in the toothpaste, all the other adjuvants are for making the conventional adjuvant of toothpaste.
Fill a prescription consumption preferably, and by weight percentage, antiinflammatory inhibiting-bacteria preparation of the present invention accounts for 0.8 ~ 1%, abrasivus 18 ~ 40%, wetting agent 18 ~ 40%, binding agent 0.8 ~ 2%, foaming agent 0.6 ~ 2.5%, correctives 0.05 ~ 0.35%, essence 1 ~ 2%, deionized water 20 ~ 35%.The conventional adjuvant component sum of antiinflammatory inhibiting-bacteria preparation of the present invention and toothpaste is 100%.
As one of embodiment, the following prescription of optional usefulness: antiinflammatory inhibiting-bacteria preparation of the present invention accounts for 2%, abrasivus 35%, wetting agent 35%, binding agent 1%, foaming agent 1%, correctives 0.2%, essence 1.8%, deionized water 24%.
It is standby that antiinflammatory inhibiting-bacteria preparation of the present invention is processed into powdery, add wetting agent and deionized water and stirring, make the deionized water suspension, mix with abrasivus, binding agent, foaming agent, correctives again, stir, grind and vacuum outgas, add essence at last and stir, grind and vacuum outgas, obtain lotion of toothpaste.
Wherein, the wetting agent of toothpaste interpolation is sorbitol, glycerol, propylene glycol, xylitol or Polyethylene Glycol (400-600).Preferably, wetting agent is glycerol, xylitol, Polyethylene Glycol.More preferably, wetting agent is xylitol.
The binding agent that toothpaste adds is sodium alginate, carboxymethyl cellulose, xanthan gum, hydroxyethyl-cellulose, guar gum, carrageenan.Preferably, binding agent is sodium alginate, carboxymethyl cellulose, hydroxyethyl-cellulose.More preferably, binding agent is carboxymethyl cellulose.
The correctives that toothpaste adds is sucrose, glucide, cyclamate, dipeptide sodium.Preferably, correctives is sucrose, glucide.More preferably, correctives is cyclamate.
The essence that toothpaste adds is Oleum menthae, lemon sesame oil, Oleum Menthae Rotundifoliae, orange oil, citrus seed oil, winter wood oil, Oleum Caryophylli, Oleum Cinnamomi, mattress Oleum sesami, Borneolum Syntheticum.Preferably, essence is Oleum menthae, lemon sesame oil, Oleum Menthae Rotundifoliae, Borneolum Syntheticum.More preferably, essence is Oleum Menthae Rotundifoliae, Oleum menthae.
The foaming agent that toothpaste adds is sodium lauryl sulphate, N-sodium lauroyl sarcosine, methyl cocoa ester group sodium taurocholate, phenolic group sodium glutamate.Preferably, foaming agent is sodium lauryl sulphate, N-sodium lauroyl sarcosine, methyl cocoa ester group sodium taurocholate.More preferably, foaming agent is sodium lauryl sulphate.
2, ameba experimental result:
In conjunction with the ameba experiment sketch map of the toothpaste of the present invention shown in Fig. 2 A, Fig. 2 B, Fig. 2 C, Fig. 2 D, the result shows: the toothpaste that contains the total ketone of antiinflammatory inhibiting-bacteria preparation active component of the present invention, total Saponin, triterpene has the obvious suppression effect to antibacterial and protozoon.
Shown in Fig. 2 A, splash into the toothpaste of using antiinflammatory inhibiting-bacteria preparation active component of the present invention before, polypide movable active, pseudopodium stretches, form is variable.
Shown in Fig. 2 B, splash into behind the toothpaste of using antiinflammatory inhibiting-bacteria preparation active component of the present invention after 1 ~ 3 second, that pseudopodium shrinks is motionless, become shape edge, garden gauffer occurs.
Shown in Fig. 2 C, splash into behind the toothpaste of using antiinflammatory inhibiting-bacteria preparation active component of the present invention after 10 seconds, Yi Bian polypide begins to split, after 22 seconds, the endoparticle blackening is a plurality of splits.
Shown in Fig. 2 D, splash into behind the toothpaste of using antiinflammatory inhibiting-bacteria preparation active component of the present invention after 30 seconds, polypide is all split, blackening, profile is uneven.
3, antiinflammatory fungistatic effect test
Strains tested: staphylococcus aureus, staphylococcus epidermidis, Streptococcus viridans, Streptococcus mutans; Above-mentioned bacterial strains is test strain, no specificity requirement, and can obtain by the conventional method separation.
Adopt the above-mentioned toothpaste of the total ketone of antiinflammatory inhibiting-bacteria preparation active component of the present invention, total Saponin, triterpene that contains of cave method or paper disk method test to the bacteriostatic activity of above-mentioned strains tested, test result is as follows:
| Strains tested | Staphylococcus aureus | Staphylococcus epidermidis | Streptococcus viridans | Streptococcus mutans |
| Inhibition zone diameter (mm) | 22 | 21 | 23 | 20 |
The result: the toothpaste that contains the total ketone of antiinflammatory inhibiting-bacteria preparation active component of the present invention, total Saponin, triterpene all more than 20mm, has fungistatic effect preferably to the above-mentioned bacterial strains inhibition zone diameter.
Therefore, use the toothpaste of antiinflammatory inhibiting-bacteria preparation active component of the present invention, it contains total ketone, total Saponin, triterpene isoreactivity composition, has that antiinflammatory is antibacterial, parasite killing and a preventing caries function.
4, clinical observation:
The toothpaste that will contain the total ketone of antiinflammatory inhibiting-bacteria preparation active component of the present invention, total Saponin, triterpene, simultaneously 235 of gingivalis Endamoeba protozoan infection persons (135 of men, 100 of woman) are established matched group in Nanfang Hospital's department of stomatology and center, oral cavity, West China, Shenzhen, use dissimilar toothpaste at random and other toothpaste of stopping using with double-blind method, check after four months, the result is as follows:
The above results confirms: the toothpaste of using antiinflammatory inhibiting-bacteria preparation active component of the present invention in the experimental group has certain pressing down to kill effect to oral cavity gingivalis Endamoeba protozoan infection person, and its effect is better than matched group.
(2), the application of antiinflammatory inhibiting-bacteria preparation of the present invention in mouth cleaner, collutory oral health care product
1, the application of antiinflammatory inhibiting-bacteria preparation of the present invention in mouth cleaner, collutory oral health care product: in mouth cleaner, collutory, add 0.5 ~ 2.0% antiinflammatory inhibiting-bacteria preparation active component of the present invention, other adjuvants are the conventional adjuvant of preparation mouth cleaner, collutory, and adopt prior preparation method and condition, can obtain to contain the total ketone of antiinflammatory inhibiting-bacteria preparation active component of the present invention, total Saponin, the mouth cleaner of triterpene, collutory.
Need to prove that the addition of described antiinflammatory inhibiting-bacteria preparation in mouth cleaner, collutory oral health care product is weight percentage 0.5 ~ 2.0%.
Preferably, the addition of described antiinflammatory inhibiting-bacteria preparation in mouth cleaner, collutory oral health care product is weight percentage 0.8 ~ 1.0%.
2, antiinflammatory fungistatic effect test
Strains tested: staphylococcus aureus, staphylococcus epidermidis, Streptococcus viridans, Streptococcus mutans; Above-mentioned bacterial strains is test strain, no specificity requirement, and can obtain by the conventional method separation.
Adopt the above-mentioned mouth cleaner that contains the total ketone of antiinflammatory inhibiting-bacteria preparation active component of the present invention, total Saponin, triterpene of paper disk method test, collutory to the bacteriostatic activity of above-mentioned strains tested, test result is as follows:
| Strains tested | Staphylococcus aureus | Staphylococcus epidermidis | Streptococcus viridans | Streptococcus mutans |
| Inhibition zone diameter (mm) | 15 | 16 | 11 | 13 |
The result: the mouth cleaner, collutory that contains the total ketone of antiinflammatory inhibiting-bacteria preparation active component of the present invention, total Saponin, triterpene all more than 10mm, has fungistatic effect preferably to the above-mentioned bacterial strains inhibition zone diameter.
(3), the application of antiinflammatory inhibiting-bacteria preparation of the present invention in individual conditioning liquid for cleaning
1, the application of antiinflammatory inhibiting-bacteria preparation of the present invention in individual conditioning liquid for cleaning: in individual conditioning liquid for cleaning, add 0.5 ~ 2.0% antiinflammatory inhibiting-bacteria preparation active component of the present invention, other adjuvants are the conventional adjuvant of the individual conditioning liquid for cleaning of preparation, and adopt prior preparation method and condition, can obtain to contain the individual conditioning liquid for cleaning of the total ketone of antiinflammatory inhibiting-bacteria preparation active component of the present invention, total Saponin, triterpene.
Need to prove that the addition of described antiinflammatory inhibiting-bacteria preparation in individual conditioning liquid for cleaning is weight percentage 0.5 ~ 2.0%.
Preferably, the addition of described antiinflammatory inhibiting-bacteria preparation in individual conditioning liquid for cleaning is weight percentage 0.8 ~ 1.0%.
2, antiinflammatory fungistatic effect test
Strains tested: staphylococcus aureus, staphylococcus epidermidis, Candida albicans; Above-mentioned bacterial strains is test strain, no specificity requirement, and can obtain by the conventional method separation.
Adopt the above-mentioned individual conditioning liquid for cleaning of the total ketone of antiinflammatory inhibiting-bacteria preparation active component of the present invention, total Saponin, triterpene that contains of paper disk method test to the bacteriostatic activity of above-mentioned strains tested, test result is as follows:
| Strains tested | Staphylococcus aureus | Staphylococcus epidermidis | ? Candida albicans |
| Inhibition zone diameter (mm) | 17 | 20 | 23 |
The result: the individual conditioning liquid for cleaning that contains the total ketone of antiinflammatory inhibiting-bacteria preparation active component of the present invention, total Saponin, triterpene all more than 10mm, has fungistatic effect preferably to the above-mentioned bacterial strains inhibition zone diameter.
(4), the application of antiinflammatory inhibiting-bacteria preparation of the present invention in the antibacterial lubricating fluid of vagina
1, the application of antiinflammatory inhibiting-bacteria preparation of the present invention in the antibacterial lubricating fluid of vagina: in the antibacterial lubricating fluid of vagina, add 0.5 ~ 2.0% antiinflammatory inhibiting-bacteria preparation active component of the present invention, other adjuvants are the conventional adjuvant of the antibacterial lubricating fluid of preparation vagina, and adopt prior preparation method and condition, can obtain to contain the antibacterial lubricating fluid of vagina of the total ketone of antiinflammatory inhibiting-bacteria preparation active component of the present invention, total Saponin, triterpene.
Need to prove that the addition of described antiinflammatory inhibiting-bacteria preparation in the antibacterial lubricating fluid of vagina is weight percentage 0.5 ~ 2.0%.
Preferably, the addition of described antiinflammatory inhibiting-bacteria preparation in the antibacterial lubricating fluid of vagina is weight percentage 0.8 ~ 1.0%.
2, antiinflammatory fungistatic effect test
Strains tested: staphylococcus aureus, staphylococcus epidermidis, Candida albicans; Above-mentioned bacterial strains is test strain, no specificity requirement, and can obtain by the conventional method separation.
Adopt the above-mentioned antibacterial lubricating fluid of vagina of the total ketone of antiinflammatory inhibiting-bacteria preparation active component of the present invention, total Saponin, triterpene that contains of paper disk method test to the bacteriostatic activity of above-mentioned strains tested, test result is as follows:
| Strains tested | Staphylococcus aureus | Staphylococcus epidermidis | ? Candida albicans |
| Inhibition zone diameter (mm) | 16 | 20 | 23 |
The result: the antibacterial lubricating fluid of vagina that contains the total ketone of antiinflammatory inhibiting-bacteria preparation active component of the present invention, total Saponin, triterpene all more than 10mm, has fungistatic effect preferably to the above-mentioned bacterial strains inhibition zone diameter.
(5), the application of antiinflammatory inhibiting-bacteria preparation of the present invention in soap sheet
1, the application of antiinflammatory inhibiting-bacteria preparation of the present invention in soap sheet: in the soap sheet raw material, add 0.5 ~ 2.0% antiinflammatory inhibiting-bacteria preparation active component of the present invention, other adjuvants are the conventional adjuvant of preparation soap sheet, and adopt prior preparation method and condition, can obtain to contain the soap sheet of the total ketone of antiinflammatory inhibiting-bacteria preparation active component of the present invention, total Saponin, triterpene.
Need to prove that the addition of described antiinflammatory inhibiting-bacteria preparation in soap sheet is weight percentage 0.5 ~ 2.0%.
Preferably, the addition of described antiinflammatory inhibiting-bacteria preparation in soap sheet is weight percentage 0.8 ~ 1.0%.
2, antiinflammatory fungistatic effect
(1) experimenter shown in Fig. 3 A, Fig. 3 B, Fig. 3 C, Fig. 3 D, Fig. 3 E, Fig. 3 F adopts the culture of bacteria of soap sheet of the present invention before and after washing one's hands to remove sketch map, above-mentioned six figure are that six experimenters adopt the soap sheet that contains the total ketone of antiinflammatory inhibiting-bacteria preparation active component of the present invention, total Saponin, triterpene, wash one's hands front and back with under the same sampling condition, culture of bacteria is removed sketch map behind palm of the hand specimen sampling, each culture dish is an experimenter from left to right, the contrast of clump count before and after expression is washed one's hands up and down.
Result: by Fig. 3 A to F as can be known, with the soap sheet of the present invention back clump count of washing one's hands obvious minimizing is arranged, and wash no residue paper scrap afterwards, compliance with environmental protection requirements with soap sheet of the present invention.
(2) antiinflammatory fungistatic effect test: respectively the experimenter is adopted soap sheet of the present invention, common soap sheet and tap water bacterial colony count and the mushroom statistics of doing experiment before and after washing one's hands respectively, specific as follows:
As seen from the above table, tap water group remains and has stayed 22.55% antibacterial; Common soap sheet group has retained 18.34% antibacterial; And soap sheet group of the present invention has only retained 1.18% antibacterial, and has removed pathogenic mushroom.Though each group all has degerming effect in various degree, soap sheet group of the present invention obviously is better than other each group.
Result: contain the soap sheet of the total ketone of antiinflammatory inhibiting-bacteria preparation active component of the present invention, total Saponin, triterpene, antibacterial, bacteria-eliminating efficacy is preferably arranged.
(6), the application of antiinflammatory inhibiting-bacteria preparation of the present invention in itch-stopping dew
1, the application of antiinflammatory inhibiting-bacteria preparation of the present invention in itch-stopping dew: in the itch-stopping dew raw material, add 0.5 ~ 2.0% antiinflammatory inhibiting-bacteria preparation active component of the present invention, other adjuvants are the conventional adjuvant of preparation itch-stopping dew, and adopt prior preparation method and condition, can obtain to contain the itch-stopping dew of the total ketone of antiinflammatory inhibiting-bacteria preparation active component of the present invention, total Saponin, triterpene.
Need to prove that the addition of described antiinflammatory inhibiting-bacteria preparation in itch-stopping dew is weight percentage 0.5 ~ 2.0%.
Preferably, the addition of described antiinflammatory inhibiting-bacteria preparation in itch-stopping dew is weight percentage 0.8 ~ 1.0%.
2, antiinflammatory fungistatic effect
(1) as shown in Figure 4, Fig. 4 is the antipruritic effect block diagram of itch-stopping dew of the present invention.Fig. 4 has shown the statistical result of using the antipruritic effect that contains itch-stopping dew of the present invention at 216 crowds that select at random, through observing confirmation itch-stopping dew of the present invention skin is crossed skin, skin pruritus, eczema, miliaria, have obvious itching-relieving efficacies with sting etc., total effective rate reaches more than 95%.
The result: the itch-stopping dew that contains the total ketone of antiinflammatory inhibiting-bacteria preparation active component of the present invention, total Saponin, triterpene has good dispelling wind for relieving itching, the antibacterial effect of antiinflammatory.
(2) antiinflammatory fungistatic effect test
Strains tested: staphylococcus aureus, staphylococcus epidermidis, Candida albicans; Above-mentioned bacterial strains is test strain, no specificity requirement, and can obtain by the conventional method separation.
Adopt the above-mentioned itch-stopping dew of the total ketone of antiinflammatory inhibiting-bacteria preparation active component of the present invention, total Saponin, triterpene that contains of paper disk method test to the bacteriostatic activity of above-mentioned strains tested, test result is as follows:
| Strains tested | Staphylococcus aureus | Staphylococcus epidermidis | ? Candida albicans |
| Inhibition zone diameter (mm) | 11 | 10 | 11 |
The result: the itch-stopping dew that contains the total ketone of antiinflammatory inhibiting-bacteria preparation active component of the present invention, total Saponin, triterpene all more than 10mm, has fungistatic effect preferably to the above-mentioned bacterial strains inhibition zone diameter.
(7), the application of antiinflammatory inhibiting-bacteria preparation of the present invention in bath gel
1, the application of antiinflammatory inhibiting-bacteria preparation of the present invention in bath gel: in bath gel, add 0.5 ~ 2.0% antiinflammatory inhibiting-bacteria preparation active component of the present invention, other adjuvants are the conventional adjuvant of preparation bath gel, and adopt prior preparation method and condition, can obtain to contain the bath gel of the total ketone of antiinflammatory inhibiting-bacteria preparation active component of the present invention, total Saponin, triterpene.
Need to prove that the addition of described antiinflammatory inhibiting-bacteria preparation in bath gel is weight percentage 0.5 ~ 2.0%.
Preferably, the addition of described antiinflammatory inhibiting-bacteria preparation in bath gel is weight percentage 0.8 ~ 1.0%.
2, antiinflammatory fungistatic effect test
Strains tested: staphylococcus aureus, staphylococcus epidermidis, Candida albicans, Streptococcus viridans; Above-mentioned bacterial strains is test strain, no specificity requirement, and can obtain by the conventional method separation.
Adopt the above-mentioned bath gel of the total ketone of antiinflammatory inhibiting-bacteria preparation active component of the present invention, total Saponin, triterpene that contains of paper disk method test to the bacteriostatic activity of above-mentioned strains tested, test result is as follows:
| Strains tested | Staphylococcus aureus | Staphylococcus epidermidis | ? Candida albicans | Streptococcus viridans |
| Inhibition zone diameter (mm) | 21 | 16 | 25 | 18 |
The result: the bath gel that contains the total ketone of antiinflammatory inhibiting-bacteria preparation active component of the present invention, total Saponin, triterpene all more than 10mm, has fungistatic effect preferably to the above-mentioned bacterial strains inhibition zone diameter.
(8), the application of antiinflammatory inhibiting-bacteria preparation of the present invention in shampoo
1, the application of antiinflammatory inhibiting-bacteria preparation of the present invention in shampoo: in shampoo, add 0.5 ~ 2.0% antiinflammatory inhibiting-bacteria preparation active component of the present invention, other adjuvants are the conventional adjuvant of preparation shampoo, and adopt prior preparation method and condition, can obtain to contain the shampoo of the total ketone of antiinflammatory inhibiting-bacteria preparation active component of the present invention, total Saponin, triterpene.
Need to prove that the addition of described antiinflammatory inhibiting-bacteria preparation in shampoo is weight percentage 0.5 ~ 2.0%.
Preferably, the addition of described antiinflammatory inhibiting-bacteria preparation in shampoo is weight percentage 0.8 ~ 1.0%.
2, antiinflammatory fungistatic effect test
Strains tested: staphylococcus aureus, staphylococcus epidermidis, Candida albicans, Streptococcus viridans; Above-mentioned bacterial strains is test strain, no specificity requirement, and can obtain by the conventional method separation.
Adopt the above-mentioned shampoo of the total ketone of antiinflammatory inhibiting-bacteria preparation active component of the present invention, total Saponin, triterpene that contains of paper disk method test to the bacteriostatic activity of above-mentioned strains tested, test result is as follows:
| Strains tested | Staphylococcus aureus | Staphylococcus epidermidis | ? Candida albicans | Streptococcus viridans |
| Inhibition zone diameter (mm) | 17 | 18 | 22 | 18 |
The result: the shampoo that contains the total ketone of antiinflammatory inhibiting-bacteria preparation active component of the present invention, total Saponin, triterpene all more than 10mm, has fungistatic effect preferably to the above-mentioned bacterial strains inhibition zone diameter.
(9), the application of antiinflammatory inhibiting-bacteria preparation of the present invention in beneficial skin frost
1, the application of antiinflammatory inhibiting-bacteria preparation of the present invention in beneficial skin frost: in beneficial skin frost, add 0.5 ~ 2.0% antiinflammatory inhibiting-bacteria preparation active component of the present invention, other adjuvants are the conventional adjuvant of the beneficial skin frost of preparation, and adopt prior preparation method and condition, can obtain to contain the beneficial skin frost of the total ketone of antiinflammatory inhibiting-bacteria preparation active component of the present invention, total Saponin, triterpene.
Need to prove that the addition of described antiinflammatory inhibiting-bacteria preparation in beneficial skin frost is weight percentage 0.5 ~ 2.0%.
Preferably, the addition of described antiinflammatory inhibiting-bacteria preparation in beneficial skin frost is weight percentage 0.8 ~ 1.0%.
2, antiinflammatory fungistatic effect test
Strains tested: staphylococcus aureus, staphylococcus epidermidis, Candida albicans; Above-mentioned bacterial strains is test strain, no specificity requirement, and can obtain by the conventional method separation.
To the bacteriostatic activity of above-mentioned strains tested, test result is as follows with the above-mentioned beneficial skin frost of the total ketone of antiinflammatory inhibiting-bacteria preparation active component of the present invention, total Saponin, triterpene that contains of cave method test in employing:
| Strains tested | Staphylococcus aureus | Staphylococcus epidermidis | ? Candida albicans |
| Inhibition zone diameter (mm) | 30 | 47 | 32 |
The result: the beneficial skin frost that contains the total ketone of antiinflammatory inhibiting-bacteria preparation active component of the present invention, total Saponin, triterpene all more than 10mm, has fungistatic effect preferably to the above-mentioned bacterial strains inhibition zone diameter.
In sum, the antiinflammatory inhibiting-bacteria preparation that the present invention will contain total ketone, total Saponin, triterpene isoreactivity composition is applied to the series of products of daily life, comprising: toothpaste; Oral health care product such as mouth cleaner, collutory; Individual's conditioning liquid for cleaning, the antibacterial lubricating fluid of vagina; Daily chemical products such as soap sheet, itch-stopping dew, bath gel, shampoo and beneficial skin frost.Because the antiinflammatory inhibiting-bacteria preparation has efficiently, wide spectrum, low drug resistance, enrich in the medicine source and characteristics such as safety coefficient height.So the described articles for use that contain the antiinflammatory inhibiting-bacteria preparation are safe and reliable, the antiinflammatory fungistatic effect is obvious.
Should be used as further explaination with specific embodiment to antiinflammatory inhibiting-bacteria preparation of the present invention below
Embodiment 1: the preparation of toothpaste of the present invention
The Ilex rotunda Thunb. bark is smashed to pieces, be 80% soak with ethanol Ilex rotunda Thunb. bark pieces with concentration, and carry out reflux, extract,, concentrated extract, 3 times of water washings are filtered, drying, it is standby to be processed into powdery---antiinflammatory inhibiting-bacteria preparation of the present invention.
Take by weighing antiinflammatory inhibiting-bacteria preparation 200g of the present invention, add glycerol 3500g, add deionized water 2000ml again and stir into suspension, add calcium hydrogen phosphate 3500g, carboxymethyl cellulose 180g, sodium lauryl sulphate 250g, cyclamate 20g, stirring, grinding, vacuum outgas add Oleum Menthae Rotundifoliae 150g agitation grinding, vacuum outgas at last, obtain lotion of toothpaste of the present invention.
Embodiment 2: the preparation of mouth cleaner of the present invention
The Ilex rotunda Thunb. bark is smashed to pieces, be 80% soak with ethanol Ilex rotunda Thunb. bark pieces with concentration, and carry out reflux, extract,, concentrated extract, 3 times of water washings are filtered, drying, it is standby to be processed into powdery---antiinflammatory inhibiting-bacteria preparation of the present invention.
Glycerol 1000g, polyoxyethylene hydrogenated Oleum Ricini 200g joined among the deionized water 8090g dissolve, add the essence solution that is dissolved in the ethanol again, wherein essence content is 80g, ethanol 500g, behind the mix homogeneously, add antiinflammatory inhibiting-bacteria preparation 100g of the present invention, saccharin sodium 15g, sodium benzoate 5g, sodium dihydrogen phosphate 10g again, dissolution filter can mouth cleaner of the present invention.
Embodiment 3: the preparation of the present invention individual conditioning liquid for cleaning
The Ilex rotunda Thunb. bark is smashed to pieces, be 80% soak with ethanol Ilex rotunda Thunb. bark pieces with concentration, and carry out reflux, extract,, concentrated extract, 3 times of water washings are filtered, drying, it is standby to be processed into powdery---antiinflammatory inhibiting-bacteria preparation of the present invention.
Add 12 carbon fatty alcohol polyoxyethylene ether 18g, dodecyldimethylammonium hydroxide inner salt 18g and pelopon A 8g, propylene glycol 16g, lactic acid 3.2g, sodium hydroxide 0.8g, dodecyl dimethyl benzyl ammonium bromide 0.5g, antiinflammatory inhibiting-bacteria preparation 10g of the present invention, add pure water then to the 900g, open and stir, and heat up;
Be cooled to 30 ℃, add vitamin A 3g, continue to stir 2 minutes, regulate pH value to 4.5 with above-mentioned bronsted lowry acids and bases bronsted lowry, and add pure water to 1000g, stir and namely got the present invention individual conditioning liquid for cleaning in 5 minutes.
Embodiment 4: the preparation of the antibacterial lubricating fluid of vagina of the present invention
The Ilex rotunda Thunb. bark is smashed to pieces, be 80% soak with ethanol Ilex rotunda Thunb. bark pieces with concentration, and carry out reflux, extract,, concentrated extract, 3 times of water washings are filtered, drying, it is standby to be processed into powdery---antiinflammatory inhibiting-bacteria preparation of the present invention.
Take by weighing vitamin E 0.6 g, vitamin A 0.09 g, vitamin D2 0 g, get liquid paraffin 100 g, said vitamin is added in the liquid paraffin lucifuge, use electronic stirring, oil solution is stirred up and down, and until evenly, other gets Mel 5g, be dissolved among sodium chloride 0.9% solution 6 g, add 1% acetum, 3 g again, and antiinflammatory inhibiting-bacteria preparation 10g of the present invention, stir jointly, adjusting this liquid pH value is 4.0 ~ 4.5, packing with flowing steam sterilization 30 minutes, obtains the antibacterial lubricating fluid of vagina of the present invention then.
Embodiment 5: the preparation of soap sheet of the present invention
The Ilex rotunda Thunb. bark is smashed to pieces, be 80% soak with ethanol Ilex rotunda Thunb. bark pieces with concentration, and carry out reflux, extract,, concentrated extract, 3 times of water washings are filtered, drying, it is standby to be processed into powdery---antiinflammatory inhibiting-bacteria preparation of the present invention.
Get following raw material: soap base (antiinflammatory inhibiting-bacteria preparation of the present invention/sulfur soap 3:97) 8g, stearic acid 2g, fatty acid distribution of coconut oil 8g, antiinflammatory inhibiting-bacteria preparation 2g of the present invention, octyl group glycerin ether sodium sulfonate 62.5g, ethylene hydroxy sulfuric acid sodium 5g, sodium chloride 4.5g, white lead 0. 5g that admires, orange bergamot essence 2g, distilled water 5. 5g;
Above-mentioned raw materials is mixed, be heated to molten condition and mix homogeneously; To obtain soap powder after the raw material spray drying; Essence is added in the soap powder, behind the spice, grind evenly; Printing shaping behind the vacuum press strip, cooling namely gets soap sheet of the present invention.
Embodiment 6: the preparation of itch-stopping dew of the present invention
The Ilex rotunda Thunb. bark is smashed to pieces, be 80% soak with ethanol Ilex rotunda Thunb. bark pieces with concentration, and carry out reflux, extract,, concentrated extract, 3 times of water washings are filtered, drying, it is standby to be processed into powdery---antiinflammatory inhibiting-bacteria preparation of the present invention.
Get 75% ethanol 9355g and add in the rustless steel container, add antiinflammatory inhibiting-bacteria preparation 100g of the present invention, stirring is dissolved it fully; In whipping process, add various essence according to this, orange blossom oil 300 g for example, Fructus Citri Limoniae oil 60 g, oleum bergamottae 120 g, geranium absolute 25 g, benzoic acid 40 g, and mix homogeneously can obtain itch-stopping dew of the present invention.
Embodiment 7: the preparation of bath gel of the present invention
The Ilex rotunda Thunb. bark is smashed to pieces, be 80% soak with ethanol Ilex rotunda Thunb. bark pieces with concentration, and carry out reflux, extract,, concentrated extract, 3 times of water washings are filtered, drying, it is standby to be processed into powdery---antiinflammatory inhibiting-bacteria preparation of the present invention.
With antiinflammatory inhibiting-bacteria preparation 200g of the present invention, fatty alcohol polyoxyethylene ether sulfate 1800g, fatty alcohol-polyoxyethylene ether sulfosuccinic acid monoesters disodium salt 800g, coconut oil aminocarbonyl propyl betanin 1000g, lauryl alcohol diethyl phthalein amine 400g mix homogeneously in reactor, and be heated to 75 ℃; After deionized water 5330 is heated to 75 ℃, join in the reactor, stir the glycerol 400g that will be heated to 75 ℃ behind the 5min and add; Stirring cools to 50 ℃ of adding citric acid 30g, Rhizoma Curcumae Longae 20g, Fructus Citri Limoniae essence 10g, methyl hydroxybenzoate 10g, and the continuation stirring is cooled to room temperature and namely gets bath gel of the present invention.
Embodiment 8: the preparation of shampoo of the present invention
The Ilex rotunda Thunb. bark is smashed to pieces, be 80% soak with ethanol Ilex rotunda Thunb. bark pieces with concentration, and carry out reflux, extract,, concentrated extract, 3 times of water washings are filtered, drying, it is standby to be processed into powdery---antiinflammatory inhibiting-bacteria preparation of the present invention.
In reactor, add deionized water 5950g, be warming up to 75 ℃.Stir on one side, Yi Bian add fatty alcohol polyoxyethylene ether sulfate 2000g, cocoanut fatty acid diethanolamide 400g successively, cocamido propyl betaine 700g, oleoyl aminoacid sodium 500g, stir speed (S.S.) is 40 rev/mins.After the uniform dissolution, add emulsified silicone oil 200g, climbazole 50g, zinc pyrithione 20g again, all after the dissolving, be cooled to 45 ℃ gradually, add antiinflammatory inhibiting-bacteria preparation 100g of the present invention, vitamin B5 50g, after evenly disperseing, add citric acid 10g, antiseptic 10g and essence 10g, continue to be stirred to evenly, obtain shampoo of the present invention.
Embodiment 9: the preparation of the present invention's benefit skin frost
The Ilex rotunda Thunb. bark is smashed to pieces, be 80% soak with ethanol Ilex rotunda Thunb. bark pieces with concentration, and carry out reflux, extract,, concentrated extract, 3 times of water washings are filtered, drying, it is standby to be processed into powdery---antiinflammatory inhibiting-bacteria preparation of the present invention.
Take by weighing following raw material components:
A component: spermol 30g, stearyl alcohol 40g, butyl stearate 80g, glycerol 50g, aliphatic alcohol polyethenoxy (25) ether 30g, lanoline 40g, D-panthenol 10g, antiinflammatory inhibiting-bacteria preparation 10g of the present invention;
B component: vitamin A 1g, vitamin E 2g, natural essence 2g, sodium benzoate 3g, methyl hydroxybenzoate 2g, distilled water 700g.
The A component slowly is heated to 80 ℃ in jacketed pan, makes it miscible; Under constantly stirring, the sodium benzoate in the B component, methyl hydroxybenzoate and distilled water evenly added in the jacketed pan mix, make it to dissolve evenly after, be cooled to 40 ℃; And then respectively will the vitamin A in the B component, vitamin E and natural essence add in the jacketed pan and mix, and obtains behind the homogenizing can dividing behind the mastic being filled in the Packaging Bottle, obtains the present invention's benefit skin frost.
In sum, implement the present invention and have following beneficial effect:
One, antiinflammatory inhibiting-bacteria preparation of the present invention is the active component that extracts from holly plant Ilex rotunda Thunb. (having another name called Cortex Ilicis Rotundae), and active component is the total ketone of 0.1 ~ 5g/100g, the total Saponin of 15 ~ 40g/100g, 45 ~ 80mg/100g triterpene.Above-mentioned active component has heat-clearing and toxic substances removing, the antibacterial effect of antiinflammatory.Therefore, antiinflammatory inhibiting-bacteria preparation of the present invention have efficiently, characteristics such as wide spectrum, low drug resistance and safety coefficient height.
Two, the preparation method of antiinflammatory inhibiting-bacteria preparation of the present invention mainly is by the effective active composition in the reflux, extract, natural plants Ilex rotunda Thunb., and its medicine source is abundant, and is simple to operate, with low cost, applied range.
Three, the present invention will contain total ketone, always the antiinflammatory inhibiting-bacteria preparation of Saponin, triterpene isoreactivity composition is applied to the series of products of daily life, comprise: toothpaste; Oral health care product such as mouth cleaner, collutory; Individual's conditioning liquid for cleaning, the antibacterial lubricating fluid of vagina; Daily chemical products such as soap sheet, itch-stopping dew, bath gel, shampoo and beneficial skin frost.And the antiinflammatory inhibiting-bacteria preparation is 0.5 ~ 2.0% to make an addition in the above-mentioned articles for use with addition.
Because the antiinflammatory inhibiting-bacteria preparation has efficiently, wide spectrum, low drug resistance, enrich in the medicine source and characteristics such as safety coefficient height.So the described articles for use that contain the antiinflammatory inhibiting-bacteria preparation are safe and reliable, the antiinflammatory fungistatic effect is obvious.
The above is preferred implementation of the present invention; should be pointed out that for those skilled in the art, under the prerequisite that does not break away from the principle of the invention; can also make some improvements and modifications, these improvements and modifications also are considered as protection scope of the present invention.
Claims (10)
1. an antiinflammatory inhibiting-bacteria preparation is characterized in that, it mainly contains following active component:
Total ketone 0.1 ~ 5g/100g
Total Saponin 15 ~ 40g/100g
Triterpene 45 ~ 80mg/100g.
2. antiinflammatory inhibiting-bacteria preparation as claimed in claim 1 is characterized in that, described antiinflammatory inhibiting-bacteria preparation mainly contains following active component:
Total ketone 2.43g/100g
Total Saponin 28.3g/100g
Triterpene 62.2mg/100g.
3. antiinflammatory inhibiting-bacteria preparation as claimed in claim 1 is characterized in that, the main active of described antiinflammatory inhibiting-bacteria preparation is extracted from Ilex rotunda Thunb. and got.
4. antiinflammatory inhibiting-bacteria preparation as claimed in claim 1 is characterized in that, described total ketone is total flavones.
5. the preparation method of each described antiinflammatory inhibiting-bacteria preparation of claim 1 ~ 4 is characterized in that, comprising:
The Ilex rotunda Thunb. bark is smashed to pieces, obtained the Ilex rotunda Thunb. bark pieces;
With the described Ilex rotunda Thunb. bark pieces of soak with ethanol, and carry out reflux, extract;
Ilex rotunda Thunb. active component concentrated extract with described reflux, extract, obtains head product;
With described head product washing, filter;
Described head product through washing filtering is carried out dried, obtain final products antiinflammatory inhibiting-bacteria preparation;
Described antiinflammatory inhibiting-bacteria preparation, it mainly contains following active component:
Total ketone 0.1 ~ 5g/100g
Total Saponin 15 ~ 40g/100g
Triterpene 45 ~ 80mg/100g.
6. each described antiinflammatory inhibiting-bacteria preparation application in toothpaste of claim 1 ~ 4.
7. each described antiinflammatory inhibiting-bacteria preparation application in mouth cleaner, collutory oral health care product of claim 1 ~ 4.
8. the application of each described antiinflammatory inhibiting-bacteria preparation of claim 1 ~ 4 in individual conditioning liquid for cleaning, the antibacterial lubricating fluid of vagina.
9. the application of each described antiinflammatory inhibiting-bacteria preparation of claim 1 ~ 4 in soap sheet, itch-stopping dew, bath gel, shampoo and beneficial skin frost day product.
10. as the application of each described antiinflammatory inhibiting-bacteria preparation of claim 6 ~ 9, it is characterized in that the addition of described antiinflammatory inhibiting-bacteria preparation in articles for use is 0.5 ~ 2.0%.
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| CN105362118A (en) * | 2015-07-14 | 2016-03-02 | 武汉华士特工业生物技术开发有限公司 | Maca health-care toothpaste and preparation method |
| CN106420420A (en) * | 2016-10-28 | 2017-02-22 | 楚雄乌蛮渡农垦有限公司 | Elsholtzia rugulosa essential oil toothpaste and preparation method thereof |
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| CN106420420A (en) * | 2016-10-28 | 2017-02-22 | 楚雄乌蛮渡农垦有限公司 | Elsholtzia rugulosa essential oil toothpaste and preparation method thereof |
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