CN102550815B - Fermented feed additive, preparation method and application - Google Patents
Fermented feed additive, preparation method and application Download PDFInfo
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- CN102550815B CN102550815B CN2011104176235A CN201110417623A CN102550815B CN 102550815 B CN102550815 B CN 102550815B CN 2011104176235 A CN2011104176235 A CN 2011104176235A CN 201110417623 A CN201110417623 A CN 201110417623A CN 102550815 B CN102550815 B CN 102550815B
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- 239000003674 animal food additive Substances 0.000 title claims abstract description 35
- 238000002360 preparation method Methods 0.000 title claims abstract description 9
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract description 39
- 241000194108 Bacillus licheniformis Species 0.000 claims abstract description 29
- 244000063299 Bacillus subtilis Species 0.000 claims abstract description 29
- 235000014469 Bacillus subtilis Nutrition 0.000 claims abstract description 29
- 241001465754 Metazoa Species 0.000 claims abstract description 29
- 240000001046 Lactobacillus acidophilus Species 0.000 claims abstract description 24
- 235000013956 Lactobacillus acidophilus Nutrition 0.000 claims abstract description 24
- 244000199866 Lactobacillus casei Species 0.000 claims abstract description 24
- 235000013958 Lactobacillus casei Nutrition 0.000 claims abstract description 24
- 229940039695 lactobacillus acidophilus Drugs 0.000 claims abstract description 24
- 229940017800 lactobacillus casei Drugs 0.000 claims abstract description 24
- 235000007164 Oryza sativa Nutrition 0.000 claims abstract description 18
- 235000009566 rice Nutrition 0.000 claims abstract description 18
- 244000046052 Phaseolus vulgaris Species 0.000 claims abstract description 11
- 235000010627 Phaseolus vulgaris Nutrition 0.000 claims abstract description 11
- 239000000835 fiber Substances 0.000 claims abstract description 9
- LPUQAYUQRXPFSQ-DFWYDOINSA-M monosodium L-glutamate Chemical compound [Na+].[O-]C(=O)[C@@H](N)CCC(O)=O LPUQAYUQRXPFSQ-DFWYDOINSA-M 0.000 claims abstract description 9
- 235000013923 monosodium glutamate Nutrition 0.000 claims abstract description 9
- 239000004223 monosodium glutamate Substances 0.000 claims abstract description 9
- 238000002156 mixing Methods 0.000 claims abstract description 8
- 240000007594 Oryza sativa Species 0.000 claims abstract 6
- 230000001580 bacterial effect Effects 0.000 claims description 65
- 241000894006 Bacteria Species 0.000 claims description 63
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims description 58
- 239000001963 growth medium Substances 0.000 claims description 52
- 230000004913 activation Effects 0.000 claims description 48
- 238000009630 liquid culture Methods 0.000 claims description 46
- 238000011081 inoculation Methods 0.000 claims description 41
- 239000002609 medium Substances 0.000 claims description 41
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 30
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 30
- 239000008103 glucose Substances 0.000 claims description 30
- 239000004310 lactic acid Substances 0.000 claims description 29
- 235000014655 lactic acid Nutrition 0.000 claims description 29
- 241000235342 Saccharomycetes Species 0.000 claims description 24
- 240000006024 Lactobacillus plantarum Species 0.000 claims description 23
- 235000013965 Lactobacillus plantarum Nutrition 0.000 claims description 23
- 229940072205 lactobacillus plantarum Drugs 0.000 claims description 23
- 239000001888 Peptone Substances 0.000 claims description 20
- 108010080698 Peptones Proteins 0.000 claims description 20
- 244000061456 Solanum tuberosum Species 0.000 claims description 20
- 235000002595 Solanum tuberosum Nutrition 0.000 claims description 20
- 235000019319 peptone Nutrition 0.000 claims description 20
- 230000001954 sterilising effect Effects 0.000 claims description 16
- 238000004659 sterilization and disinfection Methods 0.000 claims description 16
- 229920001817 Agar Polymers 0.000 claims description 15
- 239000008272 agar Substances 0.000 claims description 15
- 235000013372 meat Nutrition 0.000 claims description 15
- 239000007788 liquid Substances 0.000 claims description 13
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 claims description 10
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims description 10
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims description 10
- 235000011130 ammonium sulphate Nutrition 0.000 claims description 10
- 229940041514 candida albicans extract Drugs 0.000 claims description 10
- 239000000284 extract Substances 0.000 claims description 10
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 claims description 10
- 229920000053 polysorbate 80 Polymers 0.000 claims description 10
- 239000000843 powder Substances 0.000 claims description 10
- 239000001632 sodium acetate Substances 0.000 claims description 10
- 235000017281 sodium acetate Nutrition 0.000 claims description 10
- YWYZEGXAUVWDED-UHFFFAOYSA-N triammonium citrate Chemical compound [NH4+].[NH4+].[NH4+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O YWYZEGXAUVWDED-UHFFFAOYSA-N 0.000 claims description 10
- 239000001393 triammonium citrate Substances 0.000 claims description 10
- 235000011046 triammonium citrate Nutrition 0.000 claims description 10
- 239000012138 yeast extract Substances 0.000 claims description 10
- 229920000742 Cotton Polymers 0.000 claims description 9
- 239000002994 raw material Substances 0.000 claims description 9
- 235000015099 wheat brans Nutrition 0.000 claims description 9
- 229920002494 Zein Polymers 0.000 claims description 8
- 238000000855 fermentation Methods 0.000 claims description 8
- 230000004151 fermentation Effects 0.000 claims description 8
- 235000011389 fruit/vegetable juice Nutrition 0.000 claims description 8
- 239000005019 zein Substances 0.000 claims description 8
- 229940093612 zein Drugs 0.000 claims description 8
- 238000012856 packing Methods 0.000 claims description 6
- 238000009423 ventilation Methods 0.000 claims description 6
- 239000008367 deionised water Substances 0.000 claims description 5
- 229910021641 deionized water Inorganic materials 0.000 claims description 5
- 238000010790 dilution Methods 0.000 claims description 5
- 239000012895 dilution Substances 0.000 claims description 5
- 239000012530 fluid Substances 0.000 claims description 5
- 235000015097 nutrients Nutrition 0.000 claims description 5
- 238000003756 stirring Methods 0.000 claims description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 5
- 239000002054 inoculum Substances 0.000 claims description 2
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims 2
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims 1
- 235000019341 magnesium sulphate Nutrition 0.000 claims 1
- 239000003814 drug Substances 0.000 abstract description 11
- 239000000463 material Substances 0.000 abstract description 10
- 230000003115 biocidal effect Effects 0.000 abstract description 7
- 238000000034 method Methods 0.000 abstract description 5
- 230000008569 process Effects 0.000 abstract description 4
- 235000012343 cottonseed oil Nutrition 0.000 abstract 2
- 235000012054 meals Nutrition 0.000 abstract 2
- 241000186660 Lactobacillus Species 0.000 abstract 1
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 abstract 1
- 240000008042 Zea mays Species 0.000 abstract 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 abstract 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 abstract 1
- 235000005822 corn Nutrition 0.000 abstract 1
- 235000012631 food intake Nutrition 0.000 abstract 1
- 229940039696 lactobacillus Drugs 0.000 abstract 1
- 102000004169 proteins and genes Human genes 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
- 238000007790 scraping Methods 0.000 description 18
- 241000209094 Oryza Species 0.000 description 12
- 238000010899 nucleation Methods 0.000 description 9
- 238000012546 transfer Methods 0.000 description 9
- 241000287828 Gallus gallus Species 0.000 description 8
- 238000009360 aquaculture Methods 0.000 description 6
- 244000144974 aquaculture Species 0.000 description 6
- 238000012360 testing method Methods 0.000 description 6
- 238000002474 experimental method Methods 0.000 description 5
- 239000000203 mixture Substances 0.000 description 5
- 239000003242 anti bacterial agent Substances 0.000 description 4
- 229940088710 antibiotic agent Drugs 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 4
- 230000003053 immunization Effects 0.000 description 4
- 210000004243 sweat Anatomy 0.000 description 4
- 235000019730 animal feed additive Nutrition 0.000 description 3
- 235000013339 cereals Nutrition 0.000 description 3
- 238000011161 development Methods 0.000 description 3
- 235000013305 food Nutrition 0.000 description 3
- 102000016938 Catalase Human genes 0.000 description 2
- 108010053835 Catalase Proteins 0.000 description 2
- 102000016943 Muramidase Human genes 0.000 description 2
- 108010014251 Muramidase Proteins 0.000 description 2
- 108010062010 N-Acetylmuramoyl-L-alanine Amidase Proteins 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 230000000052 comparative effect Effects 0.000 description 2
- 230000007812 deficiency Effects 0.000 description 2
- 230000001079 digestive effect Effects 0.000 description 2
- 235000021393 food security Nutrition 0.000 description 2
- 239000004325 lysozyme Substances 0.000 description 2
- 235000010335 lysozyme Nutrition 0.000 description 2
- 229960000274 lysozyme Drugs 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- 102000040350 B family Human genes 0.000 description 1
- 108091072128 B family Proteins 0.000 description 1
- 241000589876 Campylobacter Species 0.000 description 1
- 229920000877 Melamine resin Polymers 0.000 description 1
- 241000607764 Shigella dysenteriae Species 0.000 description 1
- 241000191940 Staphylococcus Species 0.000 description 1
- 208000037386 Typhoid Diseases 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 238000003975 animal breeding Methods 0.000 description 1
- 230000008485 antagonism Effects 0.000 description 1
- -1 antibiotic Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 229940105657 catalase Drugs 0.000 description 1
- 229960001399 clenbuterol hydrochloride Drugs 0.000 description 1
- OPXKTCUYRHXSBK-UHFFFAOYSA-N clenbuterol hydrochloride Chemical compound Cl.CC(C)(C)NCC(O)C1=CC(Cl)=C(N)C(Cl)=C1 OPXKTCUYRHXSBK-UHFFFAOYSA-N 0.000 description 1
- 230000003750 conditioning effect Effects 0.000 description 1
- 239000000356 contaminant Substances 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000002158 endotoxin Substances 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 239000003205 fragrance Substances 0.000 description 1
- 230000002496 gastric effect Effects 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 210000002865 immune cell Anatomy 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- JDSHMPZPIAZGSV-UHFFFAOYSA-N melamine Chemical compound NC1=NC(N)=NC(N)=N1 JDSHMPZPIAZGSV-UHFFFAOYSA-N 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 229920006280 packaging film Polymers 0.000 description 1
- 239000012785 packaging film Substances 0.000 description 1
- 235000019629 palatability Nutrition 0.000 description 1
- 229940007046 shigella dysenteriae Drugs 0.000 description 1
- 201000008297 typhoid fever Diseases 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Landscapes
- Fodder In General (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention discloses a fermented feed additive, a preparation method and application thereof. The fermented feed additive is prepared by fermenting mixed strains and mixed auxiliary materials consisting of monosodium glutamate protein, bean pulp, bran, cottonseed meal, corn fibers, rice bran and powdered rice hulls, wherein the mixed strains are obtained by mixing activated strains of lactobacillus acidophilus, lactobacillus planetarium, lactobacillus casei, saccharomyces cerevisiae, bacillus subtilis and bacillus licheniformis in a proportion of (1-5): (1-5): (1-5): (1-5): (1-5): (1-5); and the dosage of the fermented feed additive is 5 to 100 percent of the weight of the animal feed when used for the animal feed. According to the fermented feed additive, the bean pulp, the bran, the cottonseed meal and other raw auxiliary materials are sufficiently utilized, so that the food consumption in the animal feeding process is reduced; the fermented feed additive can be used to partially or completely replace the animal feed, and antibiotic medicaments can be reduced or prevented from being used; and no medicament is left, so that the feeding cost of farmers is reduced.
Description
Technical field
The present invention relates to technical field of animal, relate in particular to a kind of feed additive.
Background technology
Along with improving constantly of people's living standard, people are more and more higher to the attention of food quality and food security.The sequence of events such as the melamine that particularly occured in recent years, clenbuterol hydrochloride cause that all the more people are to the attention of food security.Therefore, the quality as the animal feed in the source of animal food seems particularly important.At present, in the animal feeding process, need constantly to add the girth growth additives such as antibiotic, hormone, not only mouthfeel is very poor to cause the meat of producing, and contains a lot of medicament residues, increases people's the resistance to the action of a drug after the people is edible, affects the healthy of people.Therefore, the quality of production and minimizing medicament residue in order to strengthen aquaculture utilize the bioengineering means to produce a kind of antibiotic fermented feed technology that do not contain, and have become the inexorable trend of feedstuff industry.But be limited to the restriction of fermented bacterium and zymotechnique, the fermented feed technology is difficult to healthy and rapid development.
The high speed development of feedstuff industry so that feedstuff industry is more and more higher to the demand of grain raw material, even the people occurs and livestock is fought for the phenomenons such as the grain energy.The situation that disaccords in order to relax people and animals has taken full advantage of the secondary material of raw material, reduces feed cost and aquaculture cost, utilizes secondary material development fermented feed to become a kind of trend.
Summary of the invention
First technical problem to be solved by this invention is: for the deficiency that prior art exists, provide a kind of can reduce antibiotic use and stress, strengthen animal immunizing power, reduce aquaculture cost and improve the feed additive of animal meat taste.
Second technical problem to be solved by this invention is: for the deficiency of prior art existence, provide a kind of can reduce antibiotic use and stress, strengthen animal immunizing power, production cost low, reduce aquaculture cost and improve the preparation method of the feed additive of animal meat taste.
The 3rd technical problem to be solved by this invention is: provide a kind of can reduce antibiotic use and stress, the application of feed additive in animal feed that strengthen animal immunizing power, reduces aquaculture cost and improve the animal meat taste.
For solving above-mentioned first technical problem, technical scheme of the present invention is:
A kind of feed additive, formulated with the mixed accessories fermentation that comprises following raw material by mixed bacteria: monosodium glutamate albumen, dregs of beans, wheat bran, cotton dregs, zein fiber, rice bran and powdered rice hulls, described mixed bacteria are that the bacterial classification of Lactobacillus acidophilus, lactobacillus plantarum, Lactobacillus casei, S. cervisiae, bacillus subtilis and bacillus licheniformis that activation culture is obtained is mixed to get according to the ratio of 1～5:1～5:1～5:1～5:1～5:1～5.
Preferably, described mixed accessories comprises that the raw material of following percentage by weight is formulated:
Monosodium glutamate albumen 8～15%,
Dregs of beans 6～10%,
Wheat bran 10～20%,
Cotton dregs 8～12%,
Zein fiber 15～25%,
Rice bran 14～28%,
Powdered rice hulls 20～35%.
For solving above-mentioned second technical problem, technical scheme of the present invention is:
A kind of preparation method of feed additive may further comprise the steps:
(1) preparation of mixed bacteria: under aseptic condition, respectively with the bacterial classification inoculation of described Lactobacillus acidophilus, lactobacillus plantarum and Lactobacillus casei to the lactic acid bacteria activation medium, with the bacterial classification inoculation of described S. cervisiae to the saccharomycete activation medium, with the bacterial classification inoculation of described bacillus subtilis and bacillus licheniformis to the bacillus activation medium, 36～38 ℃ of activation culture 42～54 hours; The bacterial classification inoculation of the Lactobacillus acidophilus who again activation culture is obtained, lactobacillus plantarum and Lactobacillus casei is to lactic acid bacteria liquid culture medium, with the bacterial classification inoculation of described S. cervisiae to saccharomycete liquid culture medium, the bacterial classification inoculation of described bacillus subtilis and bacillus licheniformis to bacillus liquid culture medium, is enlarged at 36～38 ℃ and to cultivate 68～82 hours; Then will enlarge for the first time and cultivate the Lactobacillus acidophilus who obtains, the bacterial classification inoculation of lactobacillus plantarum and Lactobacillus casei is to lactic acid bacteria liquid culture medium, with the bacterial classification inoculation of described S. cervisiae to saccharomycete liquid culture medium, with the bacterial classification inoculation of described bacillus subtilis and bacillus licheniformis to bacillus liquid culture medium, inoculum concentration is volume ratio 8～12%, enlarge for the second time cultivation 42～54 hours at 36～38 ℃, the bacterial classification of described bacillus subtilis and bacillus licheniformis enlarges when cultivating for the first time and carries out in constant-temperature table, enlarges when cultivating for the second time and carries out under ventilation condition; Cultivate each bacterial classification of obtaining and be mixed to get mixed bacteria according to the ratio of 1～5:1～5:1～5:1～5:1～5:1～5 enlarging for the second time.
(2) mixed culture fermentation: monosodium glutamate albumen, dregs of beans, wheat bran, cotton dregs, zein fiber, rice bran and powdered rice hulls are mixed to get mixed accessories according to 8～15%, 6～10%, 10～20%, 8～12%, 15～25%, 14～28% and 20～35% weight ratio respectively; Described mixed bacteria is diluted with deionized water according to the weight ratio of 1:4～6, and adding and dilution weight ratio are that the sugar of 1～2:50 obtains mixed bacteria liquid; With described mixed bacteria liquid according to 25～35% inoculation ratio, be inoculated in the described mixed accessories and mixing and stirring, 24～26 ℃ fermented in fermenting cellar 5～7 days, obtain the feed additive product, the quantity of Lactobacillus acidophilus, lactobacillus plantarum, Lactobacillus casei, S. cervisiae, bacillus subtilis and Bacillus licheniformis strain difference 〉=0.3 * 10 in the described feed additive
8Individual/gram, 0.4 * 10
8Individual/gram, 0.2 * 10
8Individual/gram, 0.6 * 10
8Individual/gram, 0.5 * 10
8Individual/gram and 0.3 * 10
8Individual/gram.
Wherein, the prescription of described lactic acid bacteria activation medium is: peptone 10～12g/l, meat extract 10～12g/l, yeast extract 5～8g/l, K
2HPO
42～4g/l, Triammonium citrate 2～4g/l, sodium acetate 5～8g/l, glucose 20～25g/l, Tween80 1～2mL/l, MgSO
47H
2O 0.58～1g/l, MnSO
44H
2O 0.25～0.5g/l, agar 20～25g/l and CaCO
310～12g/l; The prescription of described saccharomycete activation medium is: potato 200～220g/l, glucose 20～25g/l and agar 20～25g/l; The prescription of described bacillus activation medium is: yeast soaks powder 0.7～0.9g/l, peptone 1～1.2g/l, glucose 1～1.2g/l, MgSO
40.2～0.3g/l, K
2HPO
41～1.2g/l, ammonium sulfate 0.2～0.3g/l and agar 20～25g/l.
The prescription of described lactic acid bacteria liquid culture medium is: peptone 10～12g/l, meat extract 10～12g/l, yeast extract 5～8g/l, K
2HPO
42～4g/l, Triammonium citrate 2～4g/l, sodium acetate 5～8g/l, glucose 20～25g/l, Tween80 1～2mL/l, MgSO
47H
2O 0.58～1g/l, MnSO
44H
2O 0.25～0.5g/l and CaCO
310～12g/l; The prescription of described yeast fluid nutrient medium is: potato 200～220g/l and glucose 20～25g/l; The prescription of described bacillus liquid culture medium is: yeast soaks powder 0.7～0.9g/l, peptone 1～1.2g/l, glucose 1～1.2g/l, MgSO
40.2～0.3g/l, K
2HPO
41～1.2g/l and ammonium sulfate 0.2～0.3g/l.
Preferably, when described lactic acid bacteria activation medium or lactic acid bacteria liquid culture medium prepare, described each culture medium prescription is mixed rear pH value be adjusted to 6.2～6.4,121 ℃ of sterilizations 15～20 minutes; When described saccharomycete activation medium or saccharomycete liquid culture medium prepare, described potato is prepared into potato juice mixes with each culture medium prescription again; When described bacillus activation medium or bacillus liquid culture medium prepare, described each culture medium prescription is mixed rear pH value be adjusted to 7.2～7.4,121 ℃ of sterilizations 15～20 minutes.
As a kind of improvement, after described mixed bacteria liquid and described mixed accessories mixed, put into fermenting cellar after packing with the fermented feed bag and ferment.Described fermented feed bag is the packaging film with single-way exhaust function, has another name called the one-way exhaust valve bag, use described fermented feed bag packaged fermentation feed after, both guaranteed the ventilation of sweat, also guaranteed the germ-free condition of yeasting.Described fermented feed bag is the commercially available prod, and for example Cangnan county person of outstanding talent reaches the fermented feed bag that technology of the package factory produces.
The application of a kind of animal feed additive in animal feed, the addition when described feed additive is used as animal feed is 5～100wt% of animal feed.
Owing to having adopted technique scheme, the invention has the beneficial effects as follows:
1, feed additive of the present invention, adopt the bacterial classification of Lactobacillus acidophilus, lactobacillus plantarum, Lactobacillus casei, S. cervisiae, bacillus subtilis and bacillus licheniformis as mixed bacteria, the bacterial classification of choosing can adapt to rapidly the gastric environment of strong acid, and the percent of pass and the field planting rate that enter enteron aisle are high; Can produce a large amount of probios, digestive ferment, B family vitamin, lysozyme, catalase, metabolic factor and other metabolites behind the described fermented by mixed bacterium, probio can breed rapidly after entering enteron aisle, stimulate near the immune cell propagation of enteron aisle, in enteron aisle, can effectively control endotoxin, shigella dysenteriae, typhoid bacillus, paratyphosum Bacterium, campylobacter, staphylococcus etc. there is antagonism, strengthen the immunocompetence of body, reduce the use of antibiotic medicine in the animal feeding process; Digestive ferment can promote the digest and decompose of feed, particularly to coarse-fibred decomposition utilization, improves the trans-utilization rate of feed; The growth of miscellaneous bacteria in the material such as lysozyme, catalase degradable or the inhibition enteron aisle, conditioning gut flora balance; Feed after the fermentation has unique ferment fragrance flavor, and food calling is effective, and palatability is good, can promote the animal feed.And the present invention can not discharge waste liquid and refuse in preparation process, reduced pollution and environmental protection treatment expense to environment.
2, the present invention is inoculated into mixed bacteria in the raw material auxiliary material and obtains after the fermentation, take full advantage of the raw material auxiliary materials such as dregs of beans, wheat bran, cotton dregs, reduced the grain consumption in the animal-breeding process, alleviated the predicament that people and animals disaccord, the feed additive of producing can add in the animal feed in proportion, also can all replace animal feed to use, not only can reduce or avoid using the antibiotics medicine, reduced raiser's aquaculture cost, alleviated the cultivation workload, and the animal product delicious meat of producing, and there is not medicament residue.
3, sweat of the present invention ferments after adopting fermented feed bag packing, has simplified fermentation condition, has reduced the problem that affects fermented quality in the sweat because of living contaminants, has reduced in the sweat because of the waste of mildew factor to raw material.
The specific embodiment
Below in conjunction with specific embodiment, further set forth the present invention.
Embodiment 1
Under aseptic condition, bacterial classification with described Lactobacillus acidophilus, lactobacillus plantarum and Lactobacillus casei encircles with transfer needle scraping one respectively, be seeded in the culture dish that fills the lactic acid bacteria activation medium, described lactic acid bacteria activation medium is with peptone 10g/l, meat extract 10g/l, yeast extract 5g/l, K
2HPO
42g/l, Triammonium citrate 2g/l, sodium acetate 5g/l, glucose 20g/l, Tween80 1mL/l, MgSO
47H
2O 0.58g/l, MnSO
44H
2O 0.25g/l, agar 20g/l and CaCO
3PH value was adjusted to 6.2 after 10g/l mixed, and obtained in 15 minutes 121 ℃ of sterilizations; The bacterial classification of described S. cervisiae is encircled with transfer needle scraping one, be seeded in the culture dish that fills the saccharomycete activation medium, described saccharomycete activation medium is potato 200g/l to be prepared into obtain after potato juice, glucose 20g/l and agar 20g/l mix; The bacterial classification of described bacillus subtilis and bacillus licheniformis is encircled with transfer needle scraping one, be seeded in the culture dish that fills the bacillus activation medium, described bacillus activation medium is that yeast is soaked powder 0.7g/l, peptone 1g/l, glucose 1g/l, MgSO
40.2g/l, K
2HPO
4PH value was adjusted to 7.2 after 1g/l, ammonium sulfate 0.2g/l and agar 20g/l mixed, and obtained in 15 minutes 121 ℃ of sterilizations; Each bacterial classification was 36 ℃ of activation culture 42 hours; The Lactobacillus acidophilus who again activation culture is obtained, the bacterial classification of lactobacillus plantarum and Lactobacillus casei aseptic inoculation pin scraping one pin, be seeded in the triangular flask that fills lactic acid bacteria liquid culture medium, and triangular flask placed constant-temperature table, with the bacterial classification of described S. cervisiae aseptic inoculation pin scraping one pin, be seeded in the triangular flask that fills saccharomycete liquid culture medium, with the bacterial classification of described bacillus subtilis and bacillus licheniformis aseptic inoculation pin scraping one pin, be seeded in the triangular flask that fills bacillus liquid culture medium, each bacterial classification enlarges at 36 ℃ to be cultivated 68 hours; Then will enlarge for the first time and cultivate the Lactobacillus acidophilus who obtains, the bacterial classification of lactobacillus plantarum and Lactobacillus casei is with being seeded in the seeding tank that fills lactic acid bacteria liquid culture medium, with the bacterial classification inoculation of described S. cervisiae to the seeding tank that fills saccharomycete liquid culture medium, with the bacterial classification inoculation of described bacillus subtilis and bacillus licheniformis to the seeding tank that fills bacillus liquid culture medium, each bacterial classification inoculation amount is volume ratio 8%, enlarge for the second time cultivation 42 hours at 36 ℃, wherein enlarging the second time of bacillus subtilis and bacillus licheniformis to cultivate needs to carry out under ventilation condition.Described lactic acid bacteria liquid culture medium is with peptone 10g/l, meat extract 10g/l, yeast extract 5g/l, K
2HPO
42g/l, Triammonium citrate 2g/l, sodium acetate 5g/l, glucose 20g/l, Tween80 1mL/l, MgSO
47H
2O 0.58g/l, MnSO
44H
2O 0.25g/l and CaCO
3PH value was adjusted to 6.2 after 10g/l mixed, and obtained in 15 minutes 121 ℃ of sterilizations; Described yeast fluid nutrient medium is potato 200g/l to be prepared into obtain after then potato juice mixes with glucose 20g/l; Described bacillus liquid culture medium is that yeast is soaked powder 0.7g/l, peptone 1g/l, glucose 1g/l, MgSO
40.2g/l, K
2HPO
4PH value was adjusted to 7.2 after 1g/l and ammonium sulfate 0.2g/l mixed, and obtained in 15 minutes 121 ℃ of sterilizations.Cultivate each bacterial classification of obtaining and be mixed to get mixed bacteria according to the ratio of 1:1:1:1:1:1 enlarging for the second time.
Auxiliary material monosodium glutamate albumen, dregs of beans, wheat bran, cotton dregs, zein fiber, rice bran and powdered rice hulls are mixed to get mixed accessories according to 8%, 6%, 10%, 8%, 15%, 14% and 20% weight ratio respectively; Described mixed bacteria is diluted with deionized water according to the weight ratio of 1:4, and adding and dilution weight ratio are that the sugar of 1:50 obtains mixed bacteria liquid; With described mixed bacteria liquid according to 25% inoculation ratio, be inoculated in the described mixed accessories and mixing and stirring, put into fermenting cellar after packing with the fermented feed bag, 24 ℃ fermented 5 days in fermenting cellar, obtain the feed additive product, the quantity of Lactobacillus acidophilus, lactobacillus plantarum, Lactobacillus casei, S. cervisiae, bacillus subtilis and Bacillus licheniformis strain difference 〉=0.3 * 10 in the described feed additive
8Individual/gram, 0.4 * 10
8Individual/gram, 0.2 * 10
8Individual/gram, 0.6 * 10
8Individual/gram, 0.5 * 10
8Individual/gram and 0.3 * 10
8Individual/gram.
Embodiment 2
Under aseptic condition, bacterial classification with described Lactobacillus acidophilus, lactobacillus plantarum and Lactobacillus casei encircles with transfer needle scraping one respectively, be seeded in the culture dish that fills the lactic acid bacteria activation medium, described lactic acid bacteria activation medium is with peptone 12g/l, meat extract 12g/l, yeast extract 8g/l, K
2HPO
44g/l, Triammonium citrate 4g/l, sodium acetate 8g/l, glucose 25g/l, Tween80 2mL/l, MgSO
47H
2O 1g/l, MnSO
44H
2O 0.5g/l, agar 25g/l and CaCO
3PH value was adjusted to 6.4 after 12g/l mixed, and obtained in 20 minutes 121 ℃ of sterilizations; The bacterial classification of described S. cervisiae is encircled with transfer needle scraping one, be seeded in the culture dish that fills the saccharomycete activation medium, described saccharomycete activation medium is potato 220g/l to be prepared into obtain after potato juice, glucose 25g/l and agar 25g/l mix; The bacterial classification of described bacillus subtilis and bacillus licheniformis is encircled with transfer needle scraping one, be seeded in the culture dish that fills the bacillus activation medium, described bacillus activation medium is that yeast is soaked powder 0.9g/l, peptone 1.2g/l, glucose 1.2g/l, MgSO
40.3g/l, K
2HPO
41.2g/l, ammonium sulfate 0.3g/l and agar 25g/l mix after pH value be adjusted to 7.4, obtained in 20 minutes 121 ℃ of sterilizations; Each bacterial classification was 38 ℃ of activation culture 54 hours; The Lactobacillus acidophilus who again activation culture is obtained, the bacterial classification of lactobacillus plantarum and Lactobacillus casei aseptic inoculation pin scraping one pin, be seeded in the triangular flask that fills lactic acid bacteria liquid culture medium, and triangular flask placed constant-temperature table, with the bacterial classification of described S. cervisiae aseptic inoculation pin scraping one pin, be seeded in the triangular flask that fills saccharomycete liquid culture medium, with the bacterial classification of described bacillus subtilis and bacillus licheniformis aseptic inoculation pin scraping one pin, be seeded in the triangular flask that fills bacillus liquid culture medium, each bacterial classification enlarges at 38 ℃ to be cultivated 82 hours; Then will enlarge for the first time and cultivate the Lactobacillus acidophilus who obtains, the bacterial classification of lactobacillus plantarum and Lactobacillus casei is with being seeded in the seeding tank that fills lactic acid bacteria liquid culture medium, with the bacterial classification inoculation of described S. cervisiae to the seeding tank that fills saccharomycete liquid culture medium, with the bacterial classification inoculation of described bacillus subtilis and bacillus licheniformis to the seeding tank that fills bacillus liquid culture medium, each bacterial classification inoculation amount is volume ratio 12%, enlarge for the second time cultivation 54 hours at 38 ℃, wherein enlarging the second time of bacillus subtilis and bacillus licheniformis to cultivate needs to carry out under ventilation condition.Described lactic acid bacteria liquid culture medium is with peptone 12g/l, meat extract 12g/l, yeast extract 8g/l, K
2HPO
44g/l, Triammonium citrate 4g/l, sodium acetate 8g/l, glucose 25g/l, Tween80 2mL/l, MgSO
47H
2O 1g/l, MnSO
44H
2O 0.5g/l and CaCO
3PH value was adjusted to 6.4 after 12g/l mixed, and obtained in 20 minutes 121 ℃ of sterilizations; Described yeast fluid nutrient medium is potato 220g/l to be prepared into obtain after then potato juice mixes with glucose 25g/l; Described bacillus liquid culture medium is that yeast is soaked powder 0.9g/l, peptone 1.2g/l, glucose 1.2g/l, MgSO
40.3g/l, K
2HPO
41.2g/l and pH value is adjusted to 7.4 after the ammonium sulfate 0.3g/l mixing, obtains in 20 minutes 121 ℃ of sterilizations.Cultivate each bacterial classification of obtaining and be mixed to get mixed bacteria according to the ratio of 5:5:5:5:5:5 enlarging for the second time.
Auxiliary material monosodium glutamate albumen, dregs of beans, wheat bran, cotton dregs, zein fiber, rice bran and powdered rice hulls are mixed to get mixed accessories according to 15%, 10%, 20%, 12%, 25%, 28% and 35% weight ratio respectively; Described mixed bacteria is diluted with deionized water according to the weight ratio of 1:6, and adding and dilution weight ratio are that the sugar of 2:50 obtains mixed bacteria liquid; With described mixed bacteria liquid according to 35% inoculation ratio, be inoculated in the described mixed accessories and mixing and stirring, put into fermenting cellar after packing with the fermented feed bag, 26 ℃ fermented 7 days in fermenting cellar, obtain the feed additive product, the quantity of Lactobacillus acidophilus, lactobacillus plantarum, Lactobacillus casei, S. cervisiae, bacillus subtilis and Bacillus licheniformis strain difference 〉=0.3 * 10 in the described feed additive
8Individual/gram, 0.4 * 10
8Individual/gram, 0.2 * 10
8Individual/gram, 0.6 * 10
8Individual/gram, 0.5 * 10
8Individual/gram and 0.3 * 10
8Individual/gram.
Embodiment 3
Under aseptic condition, bacterial classification with described Lactobacillus acidophilus, lactobacillus plantarum and Lactobacillus casei encircles with transfer needle scraping one respectively, be seeded in the culture dish that fills the lactic acid bacteria activation medium, described lactic acid bacteria activation medium is with peptone 11g/l, meat extract 11g/l, yeast extract 6g/l, K
2HPO
43g/l, Triammonium citrate 3g/l, sodium acetate 6g/l, glucose 22g/l, Tween80 1.5mL/l, MgSO
47H
2O 0.7g/l, MnSO
44H
2O 0.3g/l, agar 22g/l and CaCO
3PH value was adjusted to 6.3 after 11g/l mixed, and obtained in 15 minutes 121 ℃ of sterilizations; The bacterial classification of described S. cervisiae is encircled with transfer needle scraping one, be seeded in the culture dish that fills the saccharomycete activation medium, described saccharomycete activation medium is potato 210g/l to be prepared into obtain after potato juice, glucose 22g/l and agar 22g/l mix; The bacterial classification of described bacillus subtilis and bacillus licheniformis is encircled with transfer needle scraping one, be seeded in the culture dish that fills the bacillus activation medium, described bacillus activation medium is that yeast is soaked powder 0.8g/l, peptone 1.1g/l, glucose 1.1g/l, MgSO
40.25g/l, K
2HPO
41.1g/l, ammonium sulfate 0.25g/l and agar 22g/l mix after pH value be adjusted to 7.3, obtained in 15 minutes 121 ℃ of sterilizations; Each bacterial classification was 37 ℃ of activation culture 48 hours; The Lactobacillus acidophilus who again activation culture is obtained, the bacterial classification of lactobacillus plantarum and Lactobacillus casei aseptic inoculation pin scraping one pin, be seeded in the triangular flask that fills lactic acid bacteria liquid culture medium, and triangular flask placed constant-temperature table, with the bacterial classification of described S. cervisiae aseptic inoculation pin scraping one pin, be seeded in the triangular flask that fills saccharomycete liquid culture medium, with the bacterial classification of described bacillus subtilis and bacillus licheniformis aseptic inoculation pin scraping one pin, be seeded in the triangular flask that fills bacillus liquid culture medium, each bacterial classification enlarges at 37 ℃ to be cultivated 72 hours; Then will enlarge for the first time and cultivate the Lactobacillus acidophilus who obtains, the bacterial classification of lactobacillus plantarum and Lactobacillus casei is with being seeded in the seeding tank that fills lactic acid bacteria liquid culture medium, with the bacterial classification inoculation of described S. cervisiae to the seeding tank that fills saccharomycete liquid culture medium, with the bacterial classification inoculation of described bacillus subtilis and bacillus licheniformis to the seeding tank that fills bacillus liquid culture medium, each bacterial classification inoculation amount is volume ratio 10%, enlarge for the second time cultivation 48 hours at 37 ℃, wherein enlarging the second time of bacillus subtilis and bacillus licheniformis to cultivate needs to carry out under ventilation condition.Described lactic acid bacteria liquid culture medium is with peptone 11g/l, meat extract 11g/l, yeast extract 6g/l, K
2HPO
43g/l, Triammonium citrate 3g/l, sodium acetate 6g/l, glucose 22g/l, Tween80 1.5mL/l, MgSO
47H
2O 0.6g/l, MnSO
44H
2O 0.3g/l and CaCO
3PH value was adjusted to 6.3 after 11g/l mixed, and obtained in 15～20 minutes 121 ℃ of sterilizations; Described yeast fluid nutrient medium is potato 200～220g/l to be prepared into obtain after then potato juice mixes with glucose 22g/l; Described bacillus liquid culture medium is that yeast is soaked powder 0.8g/l, peptone 1.1g/l, glucose 1.1g/l, MgSO
40.25g/l, K
2HPO
41.1g/l and pH value is adjusted to 7.3 after the ammonium sulfate 0.25g/l mixing, obtains in 15 minutes 121 ℃ of sterilizations.Cultivate each bacterial classification of obtaining and be mixed to get mixed bacteria according to the ratio of 2:2:3:2:3:1 enlarging for the second time.
Auxiliary material monosodium glutamate albumen, dregs of beans, wheat bran, cotton dregs, zein fiber, rice bran and powdered rice hulls are mixed to get mixed accessories according to 10%, 8%, 15%, 10%, 20%, 20% and 25% weight ratio respectively; Described mixed bacteria is diluted with deionized water according to the weight ratio of 1:5, and adding and dilution weight ratio are that the sugar of 1:50 obtains mixed bacteria liquid; With described mixed bacteria liquid according to 30% inoculation ratio, be inoculated in the described mixed accessories and mixing and stirring, put into fermenting cellar after packing with the fermented feed bag, 25 ℃ fermented 6 days in fermenting cellar, obtain the feed additive product, the quantity of Lactobacillus acidophilus, lactobacillus plantarum, Lactobacillus casei, S. cervisiae, bacillus subtilis and Bacillus licheniformis strain difference 〉=0.3 * 10 in the described feed additive
8Individual/gram, 0.4 * 10
8Individual/gram, 0.2 * 10
8Individual/gram, 0.6 * 10
8Individual/gram, 0.5 * 10
8Individual/gram and 0.3 * 10
8Individual/gram.
Embodiment 4
The feed additive that embodiment 1, embodiment 2, embodiment 3 are obtained adds in the chicken feed according to 5% weight addition, and chicken is fed.
Embodiment 5
The feed additive that embodiment 1, embodiment 2, embodiment 3 are obtained adds in the chicken feed according to 20% weight addition, and chicken is fed.
Embodiment 6
The feed additive that embodiment 1, embodiment 2, embodiment 3 obtain is fed to chicken as the chicken feed.
The comparative example 1
Compare test, test is carried out the laying hen in 23 ages in week, to test laying hen and be divided into three groups: two groups of control group and one group of experiment, experiments, wherein control group is fed with the common chicken feed, testing one group and two groups of feed additives that obtain with embodiment 1 and embodiment 2 respectively of experiment adds in the chicken feed according to 5% weight addition and feeds, experimentation does not all use the antibiotics medicine, test period is from totally 91 days on November 25, of 27 days to 2010 August in 2010, and experimental result sees the following form.
Can find out from above comparative test result, do not using equally under the condition of antibiotics medicine, the one group of death rate with the laying hen of two groups of experiments of experiment that has added animal feed additive of the present invention is significantly less than the death rate of control group, prove absolutely that the animal feed additive that the present invention prepares can improve animal immunizing power, under the prerequisite that strengthens addition, can avoid using the antibiotics medicine fully.
Claims (3)
1. feed additive, it is characterized in that by mixed bacteria formulated with the mixed accessories fermentation that comprises following raw material: monosodium glutamate albumen, dregs of beans, wheat bran, cotton dregs, zein fiber, rice bran and powdered rice hulls, described mixed bacteria are that the bacterial classification of Lactobacillus acidophilus, lactobacillus plantarum, Lactobacillus casei, S. cervisiae, bacillus subtilis and bacillus licheniformis that activation culture is obtained is mixed to get according to the ratio of 1～5:1～5:1～5:1～5:1～5:1～5; Described mixed accessories comprises that the raw material of following percentage by weight is formulated:
The preparation method of described feed additive may further comprise the steps:
(1) preparation of mixed bacteria: under aseptic condition, respectively with the bacterial classification inoculation of described Lactobacillus acidophilus, lactobacillus plantarum and Lactobacillus casei to the lactic acid bacteria activation medium, with the bacterial classification inoculation of described S. cervisiae to the saccharomycete activation medium, with the bacterial classification inoculation of described bacillus subtilis and bacillus licheniformis to the bacillus activation medium, 36～38 ℃ of activation culture 42～54 hours; The bacterial classification inoculation of the Lactobacillus acidophilus who again activation culture is obtained, lactobacillus plantarum and Lactobacillus casei is to lactic acid bacteria liquid culture medium, with the bacterial classification inoculation of described S. cervisiae to saccharomycete liquid culture medium, the bacterial classification inoculation of described bacillus subtilis and bacillus licheniformis to bacillus liquid culture medium, is enlarged at 36～38 ℃ and to cultivate 68～82 hours; Then will enlarge for the first time and cultivate the Lactobacillus acidophilus who obtains, the bacterial classification inoculation of lactobacillus plantarum and Lactobacillus casei is to lactic acid bacteria liquid culture medium, with the bacterial classification inoculation of described S. cervisiae to saccharomycete liquid culture medium, with the bacterial classification inoculation of described bacillus subtilis and bacillus licheniformis to bacillus liquid culture medium, inoculum concentration is volume ratio 8～12%, enlarge for the second time cultivation 42～54 hours at 36～38 ℃, the bacterial classification of described bacillus subtilis and bacillus licheniformis enlarges when cultivating for the first time and carries out in constant-temperature table, enlarges when cultivating for the second time and carries out under ventilation condition; Cultivate each bacterial classification of obtaining and be mixed to get mixed bacteria according to the ratio of 1～5:1～5:1～5:1～5:1～5:1～5 enlarging for the second time;
(2) mixed culture fermentation: monosodium glutamate albumen, dregs of beans, wheat bran, cotton dregs, zein fiber, rice bran and powdered rice hulls are mixed to get mixed accessories according to 8～15%, 6～10%, 10～20%, 8～12%, 15～25%, 14～28% and 20～35% weight ratio respectively; Described mixed bacteria is diluted with deionized water according to the weight ratio of 1:4～6, and adding and dilution weight ratio are that the sugar of 1～2:50 obtains mixed bacteria liquid; With described mixed bacteria liquid according to 25～35% inoculation ratio, be inoculated in the described mixed accessories and mixing and stirring, 24～26 ℃ fermented in fermenting cellar 5～7 days, obtain the feed additive product, the quantity of Lactobacillus acidophilus, lactobacillus plantarum, Lactobacillus casei, S. cervisiae, bacillus subtilis and Bacillus licheniformis strain difference 〉=0.3 * 10 in the described feed additive
8Individual/gram, 0.4 * 10
8Individual/gram, 0.2 * 10
8Individual/gram, 0.6 * 10
8Individual/gram, 0.5 * 10
8Individual/gram and 0.3 * 10
8Individual/gram;
The prescription of described lactic acid bacteria activation medium is: peptone 10～12g/l, meat extract 10～12g/l, yeast extract 5～8g/l, K
2HPO
42～4g/l, Triammonium citrate 2～4g/l, sodium acetate 5～8g/l, glucose 20～25g/l, Tween80 1～2mL/l, MgSO
47H
2O 0.58～1g/l, MnSO
44H
2O 0.25～0.5g/l, agar 20～25g/l and CaCO
310～12g/l; The prescription of described saccharomycete activation medium is: potato 200～220g/l, glucose 20～25g/l and agar 20～25g/l; The prescription of described bacillus activation medium is: yeast soaks powder 0.7～0.9g/l, peptone 1～1.2g/l, glucose 1～1.2g/l, MgSO
40.2～0.3g/l, K
2HPO
41～1.2g/l, ammonium sulfate 0.2～0.3g/l and agar 20～25g/l;
The prescription of described lactic acid bacteria liquid culture medium is: peptone 10～12g/l, meat extract 10～12g/l, yeast extract 5～8g/l, K
2HPO
42～4g/l, Triammonium citrate 2～4g/l, sodium acetate 5～8g/l, glucose 20～25g/l, Tween80 1～2mL/l, MgSO
47H
2O 0.58～1g/l, MnSO
44H
2O 0.25～0.5g/l and CaCO
310～12g/l; The prescription of described yeast fluid nutrient medium is: potato 200～220g/l and glucose 20～25g/l; The prescription of described bacillus liquid culture medium is: yeast soaks powder 0.7～0.9g/l, peptone 1～1.2g/l, glucose 1～1.2g/l, MgSO4 0.2～0.3g/l, K
2HPO
41～1.2g/l and ammonium sulfate 0.2～0.3g/l;
When described lactic acid bacteria activation medium or lactic acid bacteria liquid culture medium prepare, described each culture medium prescription is mixed rear pH value be adjusted to 6.2～6.4,121 ℃ of sterilizations 15～20 minutes; When described saccharomycete activation medium or saccharomycete liquid culture medium prepare, described potato is prepared into potato juice mixes with each culture medium prescription again; When described bacillus activation medium or bacillus liquid culture medium prepare, described each culture medium prescription is mixed rear pH value be adjusted to 7.2～7.4,121 ℃ of sterilizations 15～20 minutes.
2. feed additive as claimed in claim 1 is characterized in that: after described mixed bacteria liquid and described mixed accessories are mixed, put into fermenting cellar after packing with the fermented feed bag and ferment.
3. the application of feed additive as claimed in claim 1 or 2 in animal feed is characterized in that: the addition when described feed additive is used as animal feed is 5～100wt% of animal feed.
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