CN106260547A - A kind of fermenting organism feedstuff and preparation method thereof - Google Patents
A kind of fermenting organism feedstuff and preparation method thereof Download PDFInfo
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Abstract
The invention belongs to Feed Manufacturing field, relate to a kind of fermenting organism feedstuff and preparation method thereof.Described fermenting organism feedstuff prepares by the following method: respectively by produce amylase bacterium, bacteria produced proteinase, acid-producing bacteria carries out the rejuvenation activation of bacterial strain, one-level spreads cultivation, it is thus achieved that produce amylase bacterium bacterium solution, bacteria produced proteinase bacterium solution, acid-producing bacteria bacterium solution；It is seeded to according to a certain percentage co-culture in culture medium by product amylase bacterium bacterium solution, bacteria produced proteinase bacterium solution, acid-producing bacteria bacterium solution, after co-culturing, obtains mixed vaccine bacterium solution；Gained mixed vaccine bacterium solution, water are together joined in fermentation raw material, carries out trough type fermentation after mix homogeneously, after fermentation ends, it is thus achieved that fermenting organism feedstuff.The fermenting organism feedstuff part using gained substitutes conventional animal feedstuff or animal feed additive, feed nutritive value and utilization rate can be effectively improved, improve the bowel problems of livestock and poultry cultivation, the enhancing cultivated animals resistivity to disease, reduce the use of antibiotic medicine.
Description
Technical field
The invention belongs to Feed Manufacturing field, relate to a kind of fermenting organism feedstuff and preparation method thereof.
Background technology
At present, livestock and poultry cultivation generally exists animal and bird intestines unhealthy, the problem of enteral nutrition absorption difference, and existing raise
In material, there is the problem utilizing rate variance in the feedstuff of 80%, poultry edible feedstuffs have 5 one-tenth more than can be because of intestinal absorption not quilt
Change into feces to discharge；Livestock and poultry pestilence takes place frequently and frequently medication simultaneously, there is the phenomenon of abuse of antibiotics, and aquaculture cost is the most therewith
Increase.Along with the release of fermenting organism feedstuff, probiotics fermented feed is considered as the preferable selection solving the problems referred to above.But it is existing
The strain for fermenting and producing biological feedstuff having all pays attention to growth performance and the acid producing ability of microorganism, the most complete so far
Face well solves the problem that livestock and poultry cultivation exists.
Summary of the invention
The present invention is directed to the deficiencies in the prior art, it is therefore intended that provide one can improve feed nutritive value and
Utilization rate, improve livestock and poultry cultivation intestinal, strengthen cultivated animals to the fermenting organism feedstuff of the resistivity of disease and preparation side thereof
Method.
For achieving the above object, the technical solution adopted in the present invention is:
The preparation method of a kind of fermenting organism feedstuff, comprises the steps:
(1) respectively by produce amylase bacterium, bacteria produced proteinase, acid-producing bacteria carries out the rejuvenation activation of bacterial strain, one-level spreads cultivation, it is thus achieved that
Produce amylase bacterium bacterium solution, bacteria produced proteinase bacterium solution, acid-producing bacteria bacterium solution；
(2) bacterial strain co-cultures: will produce amylase bacterium bacterium solution, bacteria produced proteinase bacterium solution, acid-producing bacteria bacterium solution according to a certain percentage
It is seeded to co-culture in culture medium, after co-culturing, obtains mixed vaccine bacterium solution；
(3) step (2) gained mixed vaccine bacterium solution, water are together joined in fermentation raw material, after mix homogeneously, carry out slot type
Fermentation, after fermentation ends, it is thus achieved that fermenting organism feedstuff.
In such scheme, in step (1) described product amylase bacterium bacterium solution, viable count is 1.2 × 108～2.4 × 108cfu/
ML, in described bacteria produced proteinase bacterium solution, viable count is 2.8～3.6 × 108Cfu/mL, in described acid-producing bacteria bacterium solution, viable count is
4.2×108～5.4 × 108cfu/mL。
In such scheme, step (2) described product amylase bacterium bacterium solution, bacteria produced proteinase bacterium solution and the volume of acid-producing bacteria bacterium solution
Ratio is 2～3:2～3:4～5.
In such scheme, described product amylase bacterium is bacillus cereus, and described bacteria produced proteinase is yeast, described acid-producing bacteria
For lactic acid bacteria.
In such scheme, described bacillus cereus is in bacillus subtilis, Bafillus natt and Bacillus licheniformis
One or more；Described yeast is Candida utilis and/or beer yeast；Described lactic acid bacteria is enterococcus faecalis, plant breast
One or more in acidfast bacilli and pediococcus acidilactici.
In such scheme, described in co-culture the formula of culture medium and be: 2wt ‰ brown sugar, 2wt ‰ yeast extract, surplus are water,
PH value is 7.0 ± 0.2；Or 2wt% molasses, 2wt ‰ yeast extract, surplus are water, pH value is 7.0 ± 0.2.
In such scheme, the cultivation temperature co-cultured described in step (2) is 25～30 DEG C, and incubation time is 6～8h.
In such scheme, step (3) described fermentation raw material is according to mass ratio 3～8:2 by Semen Maydis powder, bean cake powder and wheat bran
～the ratio of 4:1～3 mixes.
In such scheme, the condition of step (3) described trough type fermentation is: piling height 40～60cm；Fermentation time 60～
72h；During fermentation 15～36h, carry out 4～6 times and counter push away, follow-up will no longer carry out turning.
In such scheme, addition is fermentation raw material quality 10%～the 15% of step (3) described mixed bacteria liquid；Water
Addition is the 25%～30% of fermentation raw material quality.
The fermenting organism feedstuff that above-mentioned preparation method prepares.
Microorganism fungus kind applied in the present invention is bought by market, as bacillus subtilis is bought from " China is micro-
Biological inoculum preservation center ", Bacillus licheniformis is bought from " Chinese microorganism strain preservation center ", and Candida utilis is bought
From " Chinese microorganism strain preservation center ", beer yeast is bought from " Chinese microorganism strain preservation center ", and enterococcus faecalis is purchased
Buying from " Chinese microorganism strain preservation center ", lactobacillus plantarum is bought from " Chinese microorganism strain preservation center ", lactic acid
Sheet coccus is bought from " Chinese microorganism strain preservation center ".The original strain of purchase is carried out rejuvenation activation, one-level spreads cultivation, from
And obtain the preferable bacterium solution of thalline vigor.
The concrete operation method of described thalline rejuvenation activation is as follows:
(1) the rejuvenation activation of amylase strain is produced
A, screening and culturing based formulas: soluble starch 2wt%, peptone 1wt%, NaCl 0.5wt%, agar 2wt%,
Surplus is water, and pH value is 7.0 ± 0.2.
B, above-mentioned culture medium is configured, sterilizing 15min at 121 DEG C, be cooled to 55 DEG C, culture medium is poured into sterilizing
Culture dish in, after cooled and solidified by culture dish be inverted；Bacillus strain is diluted to identical concentration according to 10 times, aseptic
Under the conditions of the Oxford cup of prior sterilizing is spaced apart on the flat board of good culture medium according to certain, draw 0.3ml respectively
Bacterium solution is in the cup of each Oxford；After each Oxford cup adds corresponding bacterium night, culture dish is placed on the constant temperature culture of 30～35 DEG C
Middle cultivation 18 ± 2h；Take away Oxford cup and draw a small amount of I-KI solution in culture dish, shaking culture dish, make I-KI solution equal
Even it is distributed in culture dish；Now observing the size of transparent circle, the strain of selection transparent circle maximum is as dominant bacteria, by dominant bacteria
It is inoculated into one-level and spreads cultivation in culture medium, 35 ± 2 DEG C, cultivate 16h under conditions of 200 turns/min, then bacterium is inoculated into inclined-plane training night
Support 35 ± 2 DEG C of cultivation 24 ± 2h in base, then inclined-plane bacterial strain is placed in preservation in 4 DEG C of refrigerators.
The slant culture based formulas of described product amylase bacterium is: glucose 2wt%, peptone 1wt%, Carnis Bovis seu Bubali cream
0.2wt%, NaCl 0.5wt%, agar powder 15wt%, surplus are water, and pH is 7.0 ± 0.2.
The one-level of the described product amylase bacterium culture medium prescription that spreads cultivation is: glucose 2wt%, yeast extract 1wt%, NaCl
0.5wt%, surplus are water, and pH is 7.0 ± 0.2.
(2) the rejuvenation activation of bacteria produced proteinase kind
A. culture medium prescription: glucose 2wt, skimmed milk powder 1wt%, NaCl 0.3wt%, agar powder 2wt%, surplus are
Water, pH is 7.0 ± 0.2；Or glucose 2wt%, casein 2wt%, NaCl 0.3wt%, agar powder 2wt%, surplus are water,
PH is 7.0 ± 0.2.
B, above-mentioned culture medium is configured, sterilizing 15min at 105 DEG C, be cooled to 55 DEG C, culture medium is poured into sterilizing
Culture dish in, after cooled and solidified by culture dish be inverted；Yeast bacterium solution is diluted to identical concentration, aseptic bar according to 10 times
Under part, the Oxford cup of prior sterilizing is spaced apart on the flat board of good culture medium according to certain, carries out labelling；Inhale respectively
Take 0.3mL bacterium solution in the cup of each Oxford；After each Oxford cup adds corresponding bacterium night, culture dish is placed on the perseverance of 30～35 DEG C
Temperature cultivates 18 ± 2h in cultivating；Draw a small amount of card geneva light blue solution in culture dish, shake culture dish, make card geneva light blue
Solution is evenly distributed in culture dish；Now measure (slide gauge) transparent circle size, select transparent circle big as advantage
Strain, is inoculated into dominant bacteria one-level and spreads cultivation in culture medium, 35 ± 2 DEG C, cultivate 16h under conditions of 200 turns/min, then by bacterium night
It is inoculated into 35 ± 2 DEG C of cultivation 24 ± 2h in slant medium, then inclined-plane bacterial strain is placed in preservation in 4 DEG C of refrigerators.
The slant culture based formulas of described bacteria produced proteinase is: glucose 2wt%, skimmed milk powder 1wt%, NaCl
0.3wt%, agar powder 2wt%, surplus are water, and pH is 7.0 ± 0.2；Or glucose 2wt%, casein 2wt%, NaCl
0.3wt%, agar powder 2wt%, surplus are water, and pH is 7.0 ± 0.2.
The one-level of the described bacteria produced proteinase culture medium prescription that spreads cultivation is: glucose 2wt%, yeast extract 1wt%, NaCl
0.5wt%, surplus are water, and pH is 7.0 ± 0.2.
(3) the rejuvenation activation of acid-producing bacteria kind
A, culture medium prescription: glucose 2wt%, Carnis Bovis seu Bubali cream 1wt%, NaCl 0.5wt%, CaCO30.1wt%, agar
Powder 2wt%, surplus are water, and pH is 7.0 ± 0.2；Or MRS solid medium, CaCO30.1wt%, agar powder 0.5wt%, pH
It is 7.0 ± 0.2.
B, above-mentioned culture medium is configured, sterilizing 30min at 115 DEG C, be cooled to 48 ± 2 DEG C of preservations, culture medium is inclined
Note in the culture dish of sterilizing, after cooled and solidified, culture dish is inverted；Lactic acid bacterial liquid is diluted to identical dense according to 10 times
Degree, punches with hole making drill according to certain being spaced on the flat board of culture medium under aseptic condition, carries out labelling, respectively
Draw 0.3ml bacterium solution in each hole；After each hole is added corresponding bacterium night, culture dish is placed on the constant temperature culture of 35 ± 2 DEG C
Middle cultivation 36 ± 4h；After cultivation terminates, observe transparent circle size, select transparent circle big as dominant bacteria, by dominant bacteria
It is inoculated into one-level and spreads cultivation in culture medium, 35 ± 2 DEG C, cultivate 16h under quiescent conditions, then bacterium is inoculated into night in slant medium 35
Cultivate 36 ± 2h, then inclined-plane bacterial strain is placed in preservation in 4 DEG C of refrigerators for ± 2 DEG C.
Described slant culture based formulas: glucose 2wt%, peptone 1wt%, Carnis Bovis seu Bubali cream 0.2wt%, NaCl
0.5wt%, agar powder 15wt%, surplus are water, pH value 7.0 ± 0.2.
Described one-level spreads cultivation culture medium prescription: glucose 2wt%, yeast extract 1wt%, NaCl 0.5wt%, surplus are
Water, pH value is 7.0 ± 0.2.
The concrete operation method that described one-level spreads cultivation is as follows:
(1) one-level producing amylase strain spreads cultivation: the slant preservation dominant bacteria that rejuvenation activation obtains is inoculated into one-level and expands
In training culture medium, 35 ± 2 DEG C, cultivate 16h under conditions of 200 turns/min, it is thus achieved that produce amylase bacterium bacterium solution；Described product amylase bacterium
The one-level culture medium prescription that spreads cultivation be: glucose 2wt%, yeast extract 1wt%, NaCl 0.5wt%, surplus are water, and pH is
7.0±0.2。
(2) one-level of bacteria produced proteinase kind spreads cultivation: the slant preservation dominant bacteria that rejuvenation activation obtains is inoculated into one-level and expands
In training culture medium, 35 ± 2 DEG C, cultivate 16h under conditions of 200 turns/min, it is thus achieved that bacteria produced proteinase bacterium solution；Described bacteria produced proteinase
The one-level culture medium prescription that spreads cultivation be: glucose 2wt%, yeast extract 1wt%, NaCl 0.5wt%, surplus are water, and pH is
7.0±0.2。
(3) one-level of acid-producing bacteria kind spreads cultivation: the slant preservation dominant bacteria that rejuvenation activation obtains is inoculated into one-level and spreads cultivation training
Support in base, 35 ± 2 DEG C, static gas wave refrigerator 16h, it is thus achieved that acid-producing bacteria bacterium solution；Described one-level spreads cultivation culture medium prescription: glucose 2wt%,
Yeast extract 1wt%, NaCl 0.5wt%, surplus are water, and pH value is 7.0 ± 0.2.
Beneficial effects of the present invention:
(1) present invention is with Semen Maydis, bean cake, wheat bran as raw material, will produce amylase, produces protease, the sour energetic bacterial strain of product
Adding in raw material by the compatibility of science, after the outer solid fermentation of microbial body, fermentation is the most fully degraded, and fermentation
During the multiple enzyme that produces and abundant metabolite be all accumulated on fermentation material, therefore, the fermentation using gained is raw
Thing feedstuff part substitutes conventional animal feedstuff or animal feed additive, can be effectively improved feed nutritive value and utilization rate, change
The bowel problems of kind livestock and poultry cultivation, the enhancing cultivated animals resistivity to disease, reduce the use of antibiotic medicine.
(2) present invention focuses on probiotics and produces enzyme during growth metabolism and degrade fermentation raw material cell wall
Decompose with leachable；Sweat creates substantial amounts of enzyme, metabolite and cell leachable, as aminoacid,
The probiotics such as little peptide, organic acid, vitamin B group, lactic acid, butanoic acid, these materials can promote animal intestinal fine hair reparation, thus
Improve the absorption to nutrient substance, reduce animal wastes excretion；Thalline is discharged with feces simultaneously, decomposes excrement the most further
Just, can effectively reduce the concentration of ammonia, hydrogen sulfide, improve odor pollution in livestock and poultry cultivation environment；
(3) fermenting organism feedstuff of the present invention is rich in enzyme, probiotics, lactic acid, for the feedstuff or foster of this area
Growing enterprise, only need to add fermenting organism feedstuff of the present invention just can substitute for other feed additives (such as enzyme preparation, Tiny ecosystem
Preparation, acidulant, probiotics etc. feed additive) feeding effect that plays, operation sequence simplifies, is the most also avoided that because of outward
The problem adding enzyme preparation (because extra enzyme preparation contains a number of antibacterial or mycete) and bring fermentation feedstuff strain to pollute.
(4) present invention produces biological feedstuff by once inoculation mixed culture solid state fermentation, it is not necessary to fermentation, production technology by several times
Simply, properties of product are stable；Meanwhile, low cost and the production cost of fermentation raw material (Semen Maydis, bean cake, wheat bran) are low, can be foster
Grow enterprise and bring the value-rising of 10%～30%.
Detailed description of the invention
In order to be more fully understood that the present invention, it is further elucidated with present disclosure below in conjunction with embodiment, but the present invention
Content is not limited solely to the following examples.
In following example, the microorganism fungus kind applied by market buy, as bacillus subtilis buy from " in
State's Culture Collection ", Bacillus licheniformis is bought from " Chinese microorganism strain preservation center ", Candida utilis
Buying from " Chinese microorganism strain preservation center ", beer yeast is bought from " Chinese microorganism strain preservation center ", excrement intestinal ball
Bacterium is bought from " Chinese microorganism strain preservation center ", and lactobacillus plantarum is bought from " Chinese microorganism strain preservation center ",
Pediococcus acidilactici is bought from " Chinese microorganism strain preservation center ".The original strain of purchase carries out rejuvenation activation, one-level expands
Training, thus obtain the preferable bacterium solution of thalline vigor.The concrete operation method that described bacterial strain rejuvenation activation, one-level spread cultivation is interior with invention
The content recorded at appearance is identical.
Embodiment 1
A kind of fermenting organism feedstuff, is prepared via a method which to obtain:
(1) strain culturing: by bacillus subtilis, bread microzyme, enterococcus faecalis, lactobacillus plantarum, inoculate respectively
Being water to glucose 2wt%, yeast extract 1wt%, NaCl 0.5wt%, surplus, pH is in the culture medium of 7.0, hay spore
Bacillus, bread microzyme 35 DEG C, 200 turns/min cultivate 16h；Enterococcus faecalis, 35 DEG C of static gas wave refrigerator 16h of pediococcus acidilactici, obtain
(viable count is 1.4 × 10 to bacillus subtilis bacterium solution8Cfu/mL), bread microzyme bacterium solution (3.0 × 108Cfu/mL), excrement intestinal ball
(viable count is 2.3 × 10 to bacterium bacterium solution8Cfu/mL), (viable count is 3.0 × 10 to lactobacillus plantarum bacterium solution8Cfu/mL), by above-mentioned
Bacterium solution is inoculated into 2wt ‰ brown sugar, 2wt ‰ yeast extract, surplus are water, and pH is in the culture medium of 7.0, cultivates 8h, obtains for 28 DEG C
Mixed bacteria liquid；Wherein bacillus subtilis bacterium solution, bread microzyme bacterium solution, enterococcus faecalis bacterium solution, lactobacillus plantarum bacterium solution
Inoculum concentration (bacterium solution volume and the ratio of culture volume) is respectively 3%, 2%, 4%, 1%.
(2) solid fermentation inoculation: fermentation raw material (Semen Maydis powder: bean cake powder: wheat bran=5:3:2) crosses 20～40 mesh, mixing is all
Even, above-mentioned mixed bacteria liquid (10wt% of fermentation raw material quality), clear water (30wt% of fermentation raw material quality) are inoculated into fermentation
In raw material, by evenly mixing after, enter fermentation tank, ulking thickness 60cm.
(3) solid fermentation: during fermentation 12h, 18h, 24h, 36h, each turning is once, and follow-up not in turning, static gas wave refrigerator arrives
72h fermentation ends, packs.
In the present embodiment gained fermenting organism feed product: crude protein 20.67%, lactic acid 4.2%, probiotics sum 2.3
Hundred million/g, below 20KD structural polypeptide 16.4%.
Embodiment 2
A kind of fermenting organism feedstuff, is prepared via a method which to obtain:
(1) strain culturing: by Bafillus natt, Candida utilis, enterococcus faecalis, pediococcus acidilactici, connect respectively
Planting to glucose 2wt%, yeast extract 1wt%, NaCl 0.5wt%, surplus is water, and pH is in the culture medium of 7.0, natto bud
Spore bacillus, Candida utilis 36 DEG C, 200 turns/min cultivate 16h；Enterococcus faecalis, 36 DEG C of static gas wave refrigerator of pediococcus acidilactici
16h, (viable count is 2.1 × 10 to obtain Bafillus natt bacterium solution8Cfu/mL), (viable count is Candida utilis bacterium solution
2.8×108Cfu/mL), (viable count is 1.6 × 10 to enterococcus faecalis bacterium solution8Cfu/mL), (viable count is pediococcus acidilactici bacterium solution
3.5×108Cfu/mL), more above-mentioned bacterium solution is inoculated into 2wt ‰ brown sugar, 2wt ‰ yeast extract, surplus are water, pH is 7.0
In culture medium, cultivate 6h, obtain mixed bacteria liquid for 30 DEG C；Wherein Bafillus natt bacterium solution, Candida utilis bacterium solution, excrement intestinal
Coccus bacterium solution, the inoculum concentration ratio of culture volume (the bacterium solution volume with) of pediococcus acidilactici bacterium solution be respectively 3%, 2%, 4%,
1%.
(2) solid fermentation inoculation: fermentation raw material (Semen Maydis powder: bean cake powder: wheat bran=5:3:2) crosses 20～40 mesh, mixing is all
Even, above-mentioned mixed bacteria liquid (10wt% of fermentation raw material quality), clear water (30wt% of fermentation raw material quality) are inoculated into fermentation
In raw material, by evenly mixing after, enter fermentation tank, ulking thickness 50cm.
(3) solid fermentation: during fermentation 12h, 18h, 24h, 36h, each turning is once, and follow-up not in turning, static gas wave refrigerator arrives
65h fermentation ends, packs.
In the present embodiment gained fermenting organism feed product: crude protein 21.37%, lactic acid 4.8%, probiotics sum 2.8
Hundred million/g, below 20KD structural polypeptide 16.3%.
Embodiment 3
A kind of fermenting organism feedstuff, is prepared via a method which to obtain:
(1) strain culturing: by bacillus subtilis, Bacillus licheniformis, Candida utilis, enterococcus faecalis, lactic acid
Sheet coccus, is inoculated into glucose 2wt%, yeast extract 1wt%, NaCl 0.5wt%, surplus are water respectively, and pH is the training of 7.0
Supporting in base, bacillus subtilis, Bacillus licheniformis, Candida utilis 36 DEG C, 200 turns/min cultivate 16h；Excrement intestinal ball
Bacterium, 36 DEG C of static gas wave refrigerator 16h of pediococcus acidilactici, (viable count is 1.5 × 10 to obtain bacillus subtilis bacterium solution8Cfu/mL),
(viable count is 1.0 × 10 to clothing bacillus cereus bacterium solution8Cfu/mL), (viable count is 3.0 × 10 to Candida utilis bacterium solution8cfu/
ML), (viable count is 2.0 × 10 to enterococcus faecalis bacterium solution8Cfu/mL), (viable count is 3.2 × 10 to lactic acid sheet bacterium bacterium solution8Cfu/mL),
Above-mentioned bacterium solution is inoculated into 2wt% molasses again, 2wt ‰ yeast extract, surplus are water, and pH is in the culture medium of 7.0 ± 0.2,30
DEG C cultivate 7h, obtain mixed bacteria liquid；Wherein bacillus subtilis, Bacillus licheniformis, Candida utilis, enterococcus faecalis,
The inoculative proportion of lactic acid sheet bacterium is 1%, 1%, 3%, 3%, 2%.
(2) solid fermentation inoculation: fermentation raw material (Semen Maydis powder: bean cake powder: wheat bran=5:3:2) crosses 20～40 mesh, mixing is all
Even, above-mentioned mixed bacteria liquid (10wt% of fermentation raw material quality), clear water (30wt% of fermentation raw material quality) are inoculated into fermentation
In raw material, by evenly mixing after, enter fermentation tank, ulking thickness 40cm.
(3) solid fermentation: during fermentation 12h, 18h, 24h, 36h, each turning is once, and follow-up not in turning, static gas wave refrigerator arrives
60h fermentation ends, packs.
In the present embodiment gained fermenting organism feed product: crude protein 22.77%, lactic acid 4.7%, probiotics sum 3.4
Hundred million/g, below 20KD structural polypeptide 17.4%.
The present invention being prepared gained fermenting organism feedstuff be applied in pig cultivation as pig feed additive, fermenting organism is raised
The 6wt% that addition is mixed feed of material, the time fed is 26 days, feeds result as shown in table 1 below, and the result of table 1 is said
Understand: using described fermenting organism feedstuff as feed additive, be effectively increased the body weight of pig, appetite, adding weight and material meat
Ratio, effectively reduces the diarrhea rate of pig and the ammonia concentration of pig house, has and improves feedstuff palatability, increases feed nutritive value,
Promote feed digestion, improve the utilization rate of feedstuff, improve the effect of animal immunizing power.
Table 1 adds the fermenting organism feedstuff of the present invention application effect in big porker cultivation
Note: (1) this product directly adds in mixed feed according to the addition of 6%.
(2) feeding time is 26 days.
The present invention being prepared gained fermenting organism feedstuff be applied in chicken cultivation as chicken feed addictive, fermenting organism is raised
The 3wt% that addition is mixed feed of material, the time fed is 30 days, feeds result as shown in table 2 below, and the result of table 2 is said
Understand: using described fermenting organism feedstuff as feed additive, improve the laying rate of kind of chicken, rate of fertilization, incubation rate and strong young bird
Rate, the survival rate of broiler, feedstuff-meat ratio, the laying rate of laying hen and feedstuff-egg ratio, have and improve feedstuff palatability, increase feed nutrition valency
Value, promotes feed digestion, improves the utilization rate of feedstuff, improves the effect of animal immunizing power.
Table 2 adds the fermenting organism feedstuff of the present invention application effect in chicken cultivation
Note: this product directly adds in mixed feed according to the addition of 3%.
Obviously, above-described embodiment is only by clearly demonstrating made example, and not restriction to embodiment.Right
For those of ordinary skill in the field, can also make on the basis of the above description other multi-form change or
Variation.Here without also cannot all of embodiment be given exhaustive.And the obvious change therefore amplified or change
Within moving still in the protection domain of the invention.
Claims (10)
1. the preparation method of a fermenting organism feedstuff, it is characterised in that comprise the steps:
(1) respectively by produce amylase bacterium, bacteria produced proteinase, acid-producing bacteria carries out the rejuvenation activation of bacterial strain, one-level spreads cultivation, it is thus achieved that produce form sediment
Powder enzyme bacterium bacterium solution, bacteria produced proteinase bacterium solution, acid-producing bacteria bacterium solution；
(2) bacterial strain co-cultures: product amylase bacterium bacterium solution, bacteria produced proteinase bacterium solution, acid-producing bacteria bacterium solution are inoculated according to a certain percentage
To co-culturing in culture medium, after co-culturing, obtain mixed vaccine bacterium solution；
(3) step (2) gained mixed vaccine bacterium solution, water are together joined in fermentation raw material, after mix homogeneously, carry out trough type fermentation,
After fermentation ends, it is thus achieved that fermenting organism feedstuff.
Preparation method the most according to claim 1, it is characterised in that in step (1), lives in described product amylase bacterium bacterium solution
Bacterium number is 1.2 × 108~2.4×108Cfu/mL, in described bacteria produced proteinase bacterium solution, viable count is 2.8 ~ 3.6 × 108Cfu/mL,
In described acid-producing bacteria bacterium solution, viable count is 4.2 × 108~5.4×108cfu/mL。
Preparation method the most according to claim 1, it is characterised in that step (2) described product amylase bacterium bacterium solution, lay eggs white
The volume ratio of enzyme bacterium bacterium solution and acid-producing bacteria bacterium solution is 2 ~ 3:2 ~ 3:4 ~ 5.
4. according to the preparation method described in claim 1 or 2 or 3, it is characterised in that described product amylase bacterium is bacillus cereus,
Described bacteria produced proteinase is yeast, and described acid-producing bacteria is lactic acid bacteria.
Preparation method the most according to claim 4, it is characterised in that described bacillus cereus is bacillus subtilis, natto
One or more in bacillus cereus and Bacillus licheniformis；Described yeast is Candida utilis and/or beer yeast；Institute
Stating lactic acid bacteria is one or more in enterococcus faecalis, lactobacillus plantarum and pediococcus acidilactici.
Preparation method the most according to claim 1, it is characterised in that described in co-culture the formula of culture medium and be: 2wt ‰ is red
Sugar, 2wt ‰ yeast extract, surplus are water, and pH value is 7.0 ± 0.2；Or 2wt% molasses, 2wt ‰ yeast extract, surplus are water, pH
Value is 7.0 ± 0.2.
Preparation method the most according to claim 1, it is characterised in that the cultivation temperature co-cultured described in step (2) is 25 ~
30 DEG C, incubation time is 6 ~ 8h.
Preparation method the most according to claim 1, it is characterised in that step (3) described fermentation raw material is by Semen Maydis powder, bean
Dregs of rice powder and wheat bran mix according to the ratio of mass ratio 3 ~ 8:2 ~ 4:1 ~ 3.
Preparation method the most according to claim 1, it is characterised in that the condition of step (3) described trough type fermentation is: pile up
Highly 40 ~ 60cm；Fermentation time 60 ~ 72h；During fermentation 15 ~ 36h, carry out 4 ~ 6 times and counter push away, follow-up will no longer carry out turning.
10. the fermenting organism feedstuff that the arbitrary described preparation method of claim 1 ~ 9 prepares.
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| CN109527223A (en) * | 2018-12-29 | 2019-03-29 | 嘉必优生物技术（武汉）股份有限公司 | A kind of functional feed and preparation method thereof |
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