CN104322894A - Preparation method of microbial preparation for feed - Google Patents
Preparation method of microbial preparation for feed Download PDFInfo
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- CN104322894A CN104322894A CN201410712743.1A CN201410712743A CN104322894A CN 104322894 A CN104322894 A CN 104322894A CN 201410712743 A CN201410712743 A CN 201410712743A CN 104322894 A CN104322894 A CN 104322894A
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- bacillus licheniformis
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Abstract
The invention discloses a preparation method of a microbial preparation for a feed. The preparation method comprises the following steps: respectively culturing bacillus subtilis, saccharomyces cerevisiae and bacillus licheniformis till the bacterial concentration is 0.1-1*10<8>/mL, then uniformly mixing the cultured bacillus subtilis, saccharomyces cerevisiae and the bacillus licheniformis according to the strain quantity ratio of 1:(2-3):(0.1-0.5), and then inoculating into sugarcane leaves according to the weight ratio of 1:(50-100); stacking the sugarcane leaves to form a stack, covering rice bran of 1-10 centimeters on the stack, and controlling the temperature of a stack to 20-30 DEG C so as to prepare the microbial preparation for the feed after the stacking for 5-10 days. According to the preparation method, strains used during preparation are capable of effectively degrading and softening the components, namely cellulose, hemicellulose, xylogen and the like, of dried sugarcane leaves partially through synergistic effect, so that the sugarcane leaves become soft and delicious and are easily digested and absorbed by an animal; and a product is full in nutrition supply and favorable to the growth of feeding animals due to the addition of various trace elements and an enzyme agent.
Description
Technical field
The present invention relates to a kind of feed addictive, specifically a kind of preparation method of Application of Direct-fed Microbials.
Background technology
Feed addictive refers to a small amount of or micro substance added in animal feed production and processing or use procedure, it is conventional auxiliary material of throwing something and feeding in the cultivation of modern livestock, to strengthening basal feed nutritive value, improve breeding performonce fo animals, ensure animal health, save feed cost, improving the aspects such as livestock products quality has obvious effect.It is based on all kinds of chemical addition agent that existing animal feed additive mainly contains following two kinds: one, and often containing various hormone, Long-Time Service, very easily leaves medicament residue, has influence on livestock products quality, very easily threatens the health of consumer; Two is using all kinds of Chinese herbal medicine, plant extracts as master, but often expensive, or Chinese herbal medicine taste is strong, palatability extreme difference.
Summary of the invention
The object of this invention is to provide and a kind ofly to prepare simply, containing the preparation method of hormone, good palatability, Application of Direct-fed Microbials that nutritional labeling is high.
The present invention is by the following technical solutions:
A preparation method for Application of Direct-fed Microbials, comprises the following steps:
Bacillus subtilis, S. cervisiae and bacillus licheniformis are cultivated respectively, being cultured to bacterium dense is 0.1 ~ 1 × 10
8after/mL, after the bacillus subtilis after cultivation, S. cervisiae and bacillus licheniformis being mixed by 1:2 ~ 3:0.1 ~ 0.5 bacterial classification number ratio, be inoculated in sugarcane top with the weight ratio of 1:50 ~ 100, sugarcane top piles stacking, stacking covers the rice bran after 1 ~ 10cm, controlling stacking temperature is 20 ~ 30 DEG C, and stacking can obtain described Application of Direct-fed Microbials after 5 ~ 10 days.
In technique scheme:
The bacterial classification number ratio of described careless bacillus, S. cervisiae and bacillus licheniformis is preferably 1:2.5:0.3.
Described sugarcane top is preferably cut into the length of 1 ~ 5cm.Meanwhile, after the weight water content of sugarcane top is preferably adjusted to 40% ~ 50%, be used further to inoculation,
In addition, in order to make the nutritional labeling of Application of Direct-fed Microbials more comprehensive, after stacking completes, in gained material, also add the zytase of the mannonase 2% ~ 5% of its weight 1% ~ 3%, the spirulina powder of 3% ~ 6%, soybean lecithin, the vitamin C of 0.5% ~ 1%, the vitamin E of 0.1% ~ 0.5% of 0.5% ~ 5%.
Beneficial effect of the present invention is:
1, act synergistically between selected each bacterial classification during preparation, can live part degraded, the cellulose of softening dry sugarcane top, the composition such as hemicellulose and lignin, make sugarcane top become soft, agreeable to the taste, and more easily digested and assimilated by animal; 2, Application of Direct-fed Microbials is also added with rice bran, mannonase zytase, spirulina powder, soybean lecithin, vitamin C and vitamin E, making its nutrition to being comprehensive, being easy to the growth of feeding animals.
Detailed description of the invention
Be described with specific embodiment below, but the present invention is not limited to these embodiments.
Embodiment 1
The preparation method of Application of Direct-fed Microbials, comprises the following steps:
Bacillus subtilis, S. cervisiae and bacillus licheniformis are cultivated respectively, being cultured to bacterium dense is 0.5 × 10
8after/mL, by 1:2.5:0.3 bacterial classification number ratio by the bacillus subtilis after cultivation, after S. cervisiae and bacillus licheniformis mix, be inoculated in sugarcane top (sugarcane top is cut into 1 ~ 5cm and regulates water content to 45%) with the weight ratio of 1:80, sugarcane top piles stacking, stacking covers the rice bran after 5cm, controlling stacking temperature is 20 ~ 30 DEG C, stacking is after 7 days, the mannase of its weight 2% is added in gained material, the zytase of 3.5%, the spirulina powder of 4.5%, the soybean lecithin of 3%, the vitamin C of 0.7%, the vitamin E of 0.3%, described Application of Direct-fed Microbials can be obtained after mixing.
Embodiment 2
The preparation method of Application of Direct-fed Microbials, comprises the following steps:
Bacillus subtilis, S. cervisiae and bacillus licheniformis are cultivated respectively, being cultured to bacterium dense is 0.1 × 10
8after/mL, by 1:2.5:0.5 bacterial classification number ratio by the bacillus subtilis after cultivation, after S. cervisiae and bacillus licheniformis mix, be inoculated in sugarcane top (sugarcane top is cut into 1 ~ 5cm and regulates water content to 45%) with the weight ratio of 1:50, sugarcane top piles stacking, stacking covers the rice bran after 10cm, controlling stacking temperature is 20 ~ 30 DEG C, stacking is after 8 days, the mannase of its weight 3% is added in gained material, the zytase of 2%, the spirulina powder of 5%, the soybean lecithin of 5%, the vitamin C of 0.5%, the vitamin E of 0.3%, described Application of Direct-fed Microbials can be obtained after mixing.
Embodiment 3
The preparation method of Application of Direct-fed Microbials, comprises the following steps:
Bacillus subtilis, S. cervisiae and bacillus licheniformis are cultivated respectively, being cultured to bacterium dense is 0.8 × 10
8after/mL, by 1:3:0.1 bacterial classification number ratio by the bacillus subtilis after cultivation, after S. cervisiae and bacillus licheniformis mix, be inoculated in sugarcane top (sugarcane top is cut into 1 ~ 5cm and regulates water content to 50%) with the weight ratio of 1:80, sugarcane top piles stacking, stacking covers the rice bran after 1cm, controlling stacking temperature is 20 ~ 30 DEG C, stacking is after 10 days, the mannase of its weight 1% is added in gained material, the zytase of 3%, the spirulina powder of 6%, the soybean lecithin of 0.5%, the vitamin C of 0.8%, the vitamin E of 0.5%, described Application of Direct-fed Microbials can be obtained after mixing.
Embodiment 4
The preparation method of Application of Direct-fed Microbials, comprises the following steps:
Bacillus subtilis, S. cervisiae and bacillus licheniformis are cultivated respectively, being cultured to bacterium dense is 1 × 10
8after/mL, by 1:2:0.3 bacterial classification number ratio by the bacillus subtilis after cultivation, after S. cervisiae and bacillus licheniformis mix, be inoculated in sugarcane top (sugarcane top is cut into 1 ~ 5cm and regulates water content to 40%) with the weight ratio of 1:100, sugarcane top piles stacking, stacking covers the rice bran after 3cm, controlling stacking temperature is 20 ~ 30 DEG C, stacking is after 10 days, the mannase of its weight 1% is added in gained material, the zytase of 3%, the spirulina powder of 6%, the soybean lecithin of 0.5%, the vitamin C of 0.7%, the vitamin E of 0.5%, described Application of Direct-fed Microbials can be obtained after mixing.
Claims (5)
1. a preparation method for Application of Direct-fed Microbials, is characterized in that, the method comprises the following steps:
Bacillus subtilis, S. cervisiae and bacillus licheniformis are cultivated respectively, being cultured to bacterium dense is 0.1 ~ 1 × 10
8after/mL, after the bacillus subtilis after cultivation, S. cervisiae and bacillus licheniformis being mixed by 1:2 ~ 3:0.1 ~ 0.5 bacterial classification number ratio, be inoculated in sugarcane top with the weight ratio of 1:50 ~ 100, sugarcane top piles stacking, stacking covers the rice bran after 1 ~ 10cm, controlling stacking temperature is 20 ~ 30 DEG C, and stacking can obtain described Application of Direct-fed Microbials after 5 ~ 10 days.
2. preparation method according to claim 1, is characterized in that: the bacterial classification number ratio of described careless bacillus, S. cervisiae and bacillus licheniformis is 1:2.5:0.3.
3. preparation method according to claim 1, is characterized in that: described sugarcane top is cut into the length of 1 ~ 5cm.
4. preparation method according to claim 1, is characterized in that: be used further to inoculation after the weight water content of described sugarcane top is adjusted to 40% ~ 50%.
5. preparation method according to claim 1, it is characterized in that: after stacking completes, in gained material, also add the zytase of the mannonase 2% ~ 5% of its weight 1% ~ 3%, the spirulina powder of 3% ~ 6%, soybean lecithin, the vitamin C of 0.5% ~ 1%, the vitamin E of 0.1% ~ 0.5% of 0.5% ~ 5%.
Priority Applications (1)
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| CN201410712743.1A CN104322894A (en) | 2014-11-28 | 2014-11-28 | Preparation method of microbial preparation for feed |
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| CN201410712743.1A CN104322894A (en) | 2014-11-28 | 2014-11-28 | Preparation method of microbial preparation for feed |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105053727A (en) * | 2015-09-14 | 2015-11-18 | 湖州周吴鼎盛化工有限公司 | Organic feed for lambs |
| CN105076865A (en) * | 2015-09-14 | 2015-11-25 | 湖州周吴鼎盛化工有限公司 | Spirulina organic fodder for dairy cow |
| CN105124259A (en) * | 2015-09-02 | 2015-12-09 | 湖州品创孵化器有限公司 | Spirulina puffed feed for ophiocephalus argus |
| CN109601701A (en) * | 2019-01-21 | 2019-04-12 | 广西大学 | Utilize the herbvore herding special microorganism feed and production method of sugarcane end pin leaf |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1310949A (en) * | 2001-01-17 | 2001-09-05 | 中国科学院微生物研究所 | Method for producing straw protein fodder by using microbiological straw fermentation |
| CN102550815A (en) * | 2011-12-14 | 2012-07-11 | 潘玉林 | Fermented feed additive, preparation method and application |
| CN103039736A (en) * | 2012-12-19 | 2013-04-17 | 山东蔚蓝生物科技有限公司 | Three-yellow chicken diet formula with low calcium and phosphorus |
| KR101362125B1 (en) * | 2013-01-02 | 2014-02-13 | 이한별 | Nutritive fermented water, product method thereof, raising method of livestock using thereof, and meat acquired said raising method |
| CN103829073A (en) * | 2012-11-20 | 2014-06-04 | 唐蒙齐 | Method for preparing goat forage with sugarcane top as raw material |
| CN104000021A (en) * | 2014-06-11 | 2014-08-27 | 湖南伟业动物营养集团有限公司 | Processing method for plant fermented feed |
| CN104126734A (en) * | 2014-08-22 | 2014-11-05 | 成都合成生物科技有限公司 | Microecological preparation for veterinary use and preparation method thereof |
| CN104152386A (en) * | 2014-08-14 | 2014-11-19 | 河北农业大学 | Multifunctional microecological preparation |
-
2014
- 2014-11-28 CN CN201410712743.1A patent/CN104322894A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1310949A (en) * | 2001-01-17 | 2001-09-05 | 中国科学院微生物研究所 | Method for producing straw protein fodder by using microbiological straw fermentation |
| CN102550815A (en) * | 2011-12-14 | 2012-07-11 | 潘玉林 | Fermented feed additive, preparation method and application |
| CN103829073A (en) * | 2012-11-20 | 2014-06-04 | 唐蒙齐 | Method for preparing goat forage with sugarcane top as raw material |
| CN103039736A (en) * | 2012-12-19 | 2013-04-17 | 山东蔚蓝生物科技有限公司 | Three-yellow chicken diet formula with low calcium and phosphorus |
| KR101362125B1 (en) * | 2013-01-02 | 2014-02-13 | 이한별 | Nutritive fermented water, product method thereof, raising method of livestock using thereof, and meat acquired said raising method |
| CN104000021A (en) * | 2014-06-11 | 2014-08-27 | 湖南伟业动物营养集团有限公司 | Processing method for plant fermented feed |
| CN104152386A (en) * | 2014-08-14 | 2014-11-19 | 河北农业大学 | Multifunctional microecological preparation |
| CN104126734A (en) * | 2014-08-22 | 2014-11-05 | 成都合成生物科技有限公司 | Microecological preparation for veterinary use and preparation method thereof |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105124259A (en) * | 2015-09-02 | 2015-12-09 | 湖州品创孵化器有限公司 | Spirulina puffed feed for ophiocephalus argus |
| CN105053727A (en) * | 2015-09-14 | 2015-11-18 | 湖州周吴鼎盛化工有限公司 | Organic feed for lambs |
| CN105076865A (en) * | 2015-09-14 | 2015-11-25 | 湖州周吴鼎盛化工有限公司 | Spirulina organic fodder for dairy cow |
| CN109601701A (en) * | 2019-01-21 | 2019-04-12 | 广西大学 | Utilize the herbvore herding special microorganism feed and production method of sugarcane end pin leaf |
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Application publication date: 20150204 |