CN101874546A - Microorganism fermentation feed and preparation method thereof - Google Patents
Microorganism fermentation feed and preparation method thereof Download PDFInfo
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- CN101874546A CN101874546A CN 201010122093 CN201010122093A CN101874546A CN 101874546 A CN101874546 A CN 101874546A CN 201010122093 CN201010122093 CN 201010122093 CN 201010122093 A CN201010122093 A CN 201010122093A CN 101874546 A CN101874546 A CN 101874546A
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Abstract
The invention relates to a microorganism fermentation feed and a preparation method thereof. The fermentation feed comprises the following components: 400-600g of corn, 50-200g of bran, 150-250g of bean meal, 20-40g of cottonseed meal, 20-40g of rapeseed meal, 50-100g of puffed soybean meal, 20-40g of fish meal, 5g of tourmaline powder, 300-500g of pure drilled water and streptococcus acidi lactici fermented solution. The feed has the advantages of promoting animal growth, adjusting normal flora in gastrointestinal tracts, maintaining microflora balance, improving food true nutritive value and biological value, lowering serum cholesterol, controlling endotoxin, inhibiting putrefying bacteria from growth in intestinal tracts, enhancing organism immunity, having antagonistic effects on pathogenic bacterium such as dysentery bacillus, typhoid bacillus, Salmonellaparatyphi, Campylobacter, staphylococcus and the like, reducing culture cost and effectively putting antibiotics abuse to an end.
Description
Summary of the invention: the present invention relates to a kind of fermentative feedstuff of microbe and preparation method thereof.
Technical background
Entering the 21 century people has requirement more and more higher to the quality of meat product, and its requirement to nutritional quality is also more and more higher, and its source of quality of improving the meat product is quality of the fodder.Therefore the nutritional quality and the nutritional labeling that improve feed are very important for aquaculture.At present, some poultry and livestock feeds have realized that the commercialization standardization contains amino acid whose feed; Contain vitamin feed and contain antibiotic feed and contain Chinese herbal feed, these additives are low to the utilization rate of fowl poultry, contain side effect, and hazard residue enters biological chain and directly enters human body, promptly can harm humans health can work the mischief to environment again.
Summary of the invention: the purpose of this invention is to provide pollution and the disposal cost of a kind of minimizing to environment, the organic feed of antibiotic-free non agricultural chemical residuum is at fermentative feedstuff of microbe simple to operate in these sweats, with low cost and preparation method thereof.The technical scheme of employing of the present invention is as follows: a kind of fermentative feedstuff of microbe and preparation method thereof:
(1), major ingredient: corn 400-600g, wheat bran 50-200g, dregs of beans 150-250g, cotton dregs 20-40g, dish dregs of rice 20-40g, soy bean powder 50-100g, fish meal 20-40g, tourmaline powder 5g;
Bacterial classification: Lactobacillus brevis Lactobacillus brevis
Lactobacillus delbrueckii delbrueckii subsp.Lactis
Lactobacillus casei paracasei
Lactococcus lactis Lactobacillus brevis
Lactobacillus pentosus (Lactobacilluspentosaceus)
Bu Shi lactobacillus (Lactobacillus)
Lactobacillus plantarum (Lactobacillusplantarum)
Lactobacillus acetotolerans (Lactobacillus acetotolerans)
More than each bacterial classification be by separating the lactic acid bacteria in pig manure, the chitling road, in the fermented feed, a single bacterium colony choosing Lactobacillus brevis, Lactobacillus delbrueckii, Lactobacillus casei, Lactococcus lactis, Lactobacillus pentosus, Bu Shi lactobacillus, Lactobacillus plantarum, each bacterial strain of lactobacillus acetotolerans bacterial strain picking of high-quality wherein carries out the inclined-plane purifying and cultivates and obtain;
(2), the culture medium of an activation culture of preparation: soy peptone 10g, powdered beef 6g, dusty yeast 6g, glucose 40g, lactose 40g, distilled water 200ml, the above each component of weighing also is dissolved in the 200ml distilled water, the culture medium of an activation culture configuring is packed in the triangular flask of 400ml, under 121 degrees celsius, behind the autoclaving 20min, the refrigerator of putting into-4 degrees centigrade is stand-by;
An activation culture; A single bacterium colony on the inclined-plane such as anti-, the Lactobacillus lactis bacterial classification of picking Lactobacillus brevis, Lactobacillus delbrueckii, Lactobacillus casei, Lactococcus lactis, Lactobacillus pentosus, Bush's lactobacillus, Lactobacillus plantarum respectively, it is inoculated into respectively in activation medium of 20ml, under 40 ℃ of conditions of temperature, the static cultivation of anaerobism 12-18h, between the ph of an activation culture reaches 3.8～4.5 and tunning when having acid fragrant, be and reach ferment effect;
(3), the preparation secondary enlarges culture medium: soy peptone 1kg, deionized water 10kg, sodium chloride 200g, each component of weighing is dissolved in it in 10kg deionized water, under 118 degrees celsius, high pressure steam sterilization 20min, the refrigerator of putting into-4 degrees centigrade is stand-by; Secondary enlarges to be cultivated; Bacterium liquid (each 20ml) co-inoculation of an activation culture of each bacterial strain secondary to 10kg is enlarged in the culture medium, under 40 ℃ of conditions, the static cultivation of anaerobism 24h between two ph that enlarge to cultivate reach 3.8～4.5 and tunning when having acid fragrant, is and reaches ferment effect;
(4), preparation enlarges culture medium three times: brown sugar 350g sodium chloride 10g, deionized water 350kg, each component of weighing is dissolved in it in deionized water of 350kg, high pressure steam sterilization 20min under 121 degrees celsius, enlarge for three times and cultivate: secondary is enlarged the nutrient solution 10kg that cultivates be inoculated in 350kg three subcultures, in temperature is under 40 degrees celsius, the static cultivation of anaerobism 24h;
(5) fermented feed: the bacterium liquid 10kg that three expansion is cultivated, brown sugar 250g, deionized water 100kg, major ingredient 1000kg stirs above each component machine with small-sized stirring, the feed to be fermented that stirs is packed in the airtight 100kg bucket, and with the bucket good seal, room temperature remains on about 25 ℃, sealing and fermenting promptly got product in 8 hours, when fermented feed has biscuit fragrance.
Technique effect of the present invention is can not discharge waste liquid and refuse in the sweat.Minimizing is to the pollution and the disposal cost of environment.An activation culture of culture medium, secondary enlarge the organic feed of cultivating, enlarge for three times cultivation and antibiotic-free non agricultural chemical residuum and obtain fermentative feedstuff of microbe at the oxygen free condition bottom fermentation, simple to operate in these sweats, with low cost, be fit to breeding enterprise and use.And in the process of continuously fermenting, secondary enlarges to contain in the zymotic fluid of cultivating much enzyme and growth factor, it is joined to enlarge for three times in the culture mediums can under lower nutritional condition, continue to keep the bacterium normal growth, this is outstanding having a few of the present invention, has so not only reduced the production cost of large scale fermentation but also the zymotic fluid that can obtain having certain activity.
The microorganism of fowl poultry enteron aisle and feed digested and assimilated confidential relation.When utilizing this lactobacillus-fermented feed to feed and zymotic fluid drink can make when feeding animal in the enteron aisle beneficial flora just often, and lactic acid bacteria promptly can be improved food digestion rate and biological value; The control endotoxin; Suppress corrupt bacteria growing in the enteron aisle.The biological rhzomorph that lactic acid bacteria metabolism simultaneously produces has antagonism to do for pathogenic bacteria such as shigella dysenteriae, typhoid bacillus, paratyphosum Bacterium, campylobacter, grape ball Lin etc., improves immunity of organisms etc.
Description of drawings:
Fig. 1 is the viable bacteria numerical table that is contained among three nutrient solution 1ml
The viable bacteria numerical table of Fig. 2 for being contained among the fermented feed 1g
Fig. 3 is the table of comparisons of main nutrient composition protein in the fermented feed, fat and unleavened feed main nutrient composition
Fig. 4 is the contrast test table of feeding with fermented feed of the present invention
The specific embodiment:
Embodiment 1
1. (1), major ingredient: corn 400-600g, wheat bran 50-200g, dregs of beans 150-250g, cotton dregs 20-40g, dish dregs of rice 20-40g, soy bean powder 50-100g, fish meal 20-40g, tourmaline powder 5g;
Bacterial classification: Lactobacillus brevis Lactobacillus brevis
Lactobacillus delbrueckii delbrueckii subsp.Lactis
Lactobacillus casei paracasei
Lactococcus lactis Lactobacillus brevis
Lactobacillus pentosus (Lactobacilluspentosaceus)
Bu Shi lactobacillus (Lactobacillus)
Lactobacillus plantarum (Lactobacillusplantarum)
Lactobacillus acetotolerans (Lactobacillus acetotolerans)
Separate in pig manure, the chitling road, the lactic acid bacteria in the fermented feed, choose quality strains Lactobacillus brevis, Lactobacillus delbrueckii, Lactobacillus casei, Lactococcus lactis, Lactobacillus pentosus, Bu Shi lactobacillus, Lactobacillus plantarum, each bacterial strain of lactobacillus acetotolerans picking a single bacterium bacterium colony carry out inclined-plane purifying cultivation.
The culture medium of an activation culture of preparation: soy peptone 10g, powdered beef 6g, dusty yeast 6g, glucose 40g, lactose 40g, distilled water 200ml, each component is mixed, after pack into and 500 pack in the triangular flask, high pressure steam sterilization 15mi under 121 degrees celsius, the refrigerator of putting into-4 degrees centigrade is stand-by.
1. activation culture; Use the bacterium colony on the inclined-planes such as 1ml physiological saline wash-out Lactobacillus brevis, Lactobacillus delbrueckii, Lactobacillus casei, Lactococcus lactis, Lactobacillus pentosus, Bush's lactobacillus, Lactobacillus plantarum are anti-, Lactobacillus lactis respectively, each eluent that elutes is inoculated into respectively in activation medium of 20ml, under 40 ℃ of conditions of temperature, the static cultivation of anaerobism 12-18h;
2. the preparation secondary enlarges culture medium: soy peptone 1kg deionized water 10kg sodium chloride 200g will mix with last component, joined in the small-sized fermentation jar of 20kg capacity, and high pressure steam sterilization 15min is stand-by under 118 degrees celsius.
3. secondary enlarges cultivation; The bacterium liquid of each bacterial strain that activation culture is obtained joins secondary simultaneously and enlarges in the culture medium of cultivating under 40 ℃ of conditions the static cultivation of anaerobism 24h.
4. prepare and enlarge culture medium for three times: join behind the brown sugar 350g sodium chloride 10g deionized water 350kg dissolving component in 3501 the large fermentation tank, under 121 degrees celsius, high pressure steam sterilization 15min, stand-by.
5. enlarge for three times and cultivate: inoculation secondary expansion nutrient solution 20kg is inoculated in three expansion culture mediums of 350kg, and under 40 degrees celsius, static anaerobism is cultivated 24h.
8. cultivating bacterial activity for three times identifies and carries out colony counting (national standard of the total assay method of lactic acid bacteria is carried out in the test method reference food) with the ATJ culture medium measurement result as shown in Figure 1.
6. complete feed fermentation; The three zymophyte 10kg brown sugar 100g deionized water 100kg major ingredient 1000kg that enlarge to cultivate utilize pony mixer to stir the fermented feed that stirs is packed in the sealed fermenting bucket of 100kg, under about 25 ℃ the condition, sealing and fermenting promptly got slightly acidic flavoured prod of biscuit fragrance in 8 hours in the holding chamber.
7. the fermented feed probiotic active is identified with the ATJ culture medium and is carried out shown in colony counting (national standard of lactic acid bacteria sum assay method is carried out in the test method reference food) the measurement result accompanying drawing 2.
8. the contrast of main nutrient composition protein, fat and unleavened feed main nutrient composition in the mensuration fermented feed, measurement result as shown in Figure 3.
Embodiment 2
The zymotic fluid drink is fed test
Enlarge the bacterium liquid of cultivating with three times of the present invention and carried out drink hello test
1. enlarging the bacteria solution active of cultivating for three times measures: measurement result as shown in Figure 1 to carry out colony counting (national standard of lactic acid bacteria sum assay method is carried out in the test method reference food) with the ATJ culture medium.
2. dilute three times and enlarge the bacterium liquid of cultivating: according to the bacterium activity in the bacterium liquid of three expansion cultivations, enlarge the bacterium liquid of cultivating three times with the deionized water dilution, dilution ratio is 1: 5.
3. drink is fed method: drink is fed every day and is fed by 4 drinks, and the time period is respectively 4:00 early; The late 6:00 of 10:00 5:00 in afternoon at noon; Every each drink of pig is fed about 500ml;
Embodiment 3
The fermented feed feeding experiment
With the fermented feed of the present invention contrast test of feeding, to 100 growing and fattening pigs pig live bodies carried out feeding experiment wherein 50 be test group this lactobacillus-fermented feed of feeding, 50 feeds that are control group fed without lactobacillus-fermented.Feeding method is that four times a day feeding time section is respectively early that 3:00 9:00 at noon 4:00 in afternoon 22:00 in evening feeds about 500g at every turn.Feed through 150 days, statistical experiment result, measurement result are as shown in Figure 4.
Claims (1)
1. fermentative feedstuff of microbe and preparation method thereof is characterized in that: (1), major ingredient: corn 400-600g, wheat bran 50-200g, dregs of beans 150-250g, cotton dregs 20-40g, dish dregs of rice 20-40g, soy bean powder 50-100g, fish meal 20-40g, tourmaline powder 5g;
Bacterial classification: Lactobacillus brevis Lactobacillus brevis
Lactobacillus delbrueckii delbrueckii subsp.Lactis
Lactobacillus casei paracasei
Lactococcus lactis Lactobacillus brevis
Lactobacillus pentosus (Lactobacilluspentosaceus)
Bu Shi lactobacillus (Lactobacillus)
Lactobacillus plantarum (Lactobacillusplantarum)
Lactobacillus acetotolerans (Lactobacillus acetotolerans)
More than each bacterial classification be by separating the lactic acid bacteria in pig manure, the chitling road, in the fermented feed, a single bacterium colony choosing Lactobacillus brevis, Lactobacillus delbrueckii, Lactobacillus casei, Lactococcus lactis, Lactobacillus pentosus, Bu Shi lactobacillus, Lactobacillus plantarum, each bacterial strain of lactobacillus acetotolerans bacterial strain picking of high-quality wherein carries out the inclined-plane purifying and cultivates and obtain;
(2), the culture medium of an activation culture of preparation: soy peptone 10g, powdered beef 6g, dusty yeast 6g, glucose 40g, lactose 40g, distilled water 200ml, the above each component of weighing also is dissolved in the 200ml distilled water, the culture medium of an activation culture configuring is packed in the triangular flask of 400ml, under 121 degrees celsius, behind the autoclaving 20min, the refrigerator of putting into-4 degrees centigrade is stand-by;
An activation culture; A single bacterium colony on the inclined-plane such as anti-, the Lactobacillus lactis bacterial classification of picking Lactobacillus brevis, Lactobacillus delbrueckii, Lactobacillus casei, Lactococcus lactis, Lactobacillus pentosus, Bush's lactobacillus, Lactobacillus plantarum respectively, it is inoculated into respectively in activation medium of 20ml, under 40 ℃ of conditions of temperature, the static cultivation of anaerobism 12-18h, between the ph of an activation culture reaches 3.8～4.5 and tunning when having acid fragrant, be and reach ferment effect;
(3), the preparation secondary enlarges culture medium: soy peptone 1kg, deionized water 10kg, sodium chloride 200g, each component of weighing is dissolved in it in 10kg deionized water, under 118 degrees celsius, high pressure steam sterilization 20min, the refrigerator of putting into-4 degrees centigrade is stand-by; Secondary enlarges to be cultivated; Bacterium liquid (each 20ml) co-inoculation of an activation culture of each bacterial strain secondary to 10kg is enlarged in the culture medium, under 40 ℃ of conditions, the static cultivation of anaerobism 24h between two ph that enlarge to cultivate reach 3.8～4.5 and tunning when having acid fragrant, is and reaches ferment effect;
(4), preparation enlarges culture medium three times: brown sugar 350g sodium chloride 10g, deionized water 350kg, each component of weighing is dissolved in it in deionized water of 350kg, high pressure steam sterilization 20min under 121 degrees celsius, enlarge for three times and cultivate: secondary is enlarged the nutrient solution 10kg that cultivates be inoculated in 350kg three subcultures, in temperature is under 40 degrees celsius, the static cultivation of anaerobism 24h;
(5) fermented feed: the bacterium liquid 10kg that three expansion is cultivated, brown sugar 250g, deionized water 100kg, major ingredient 1000kg stirs above each component machine with small-sized stirring, the feed to be fermented that stirs is packed in the airtight 100kg bucket, and with the bucket good seal, room temperature remains on about 25 ℃, sealing and fermenting promptly got product in 8 hours, when fermented feed has biscuit fragrance.
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Cited By (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102987435A (en) * | 2012-11-13 | 2013-03-27 | 西华大学 | Preparation method for multifunctional complex-fermented powder for tenderization and fermentation and method for preparing fermented yak meat particles |
| CN102994420A (en) * | 2012-11-09 | 2013-03-27 | 黑龙江八一农垦大学 | Low-temperature resistant lactobacillus with ACE (angiotensin converting enzyme) inhibitory activity |
| CN103070287A (en) * | 2013-02-26 | 2013-05-01 | 湖南省微生物研究所 | Lactobacillus paracasei fermented feed and preparation method thereof, fermenting equipment and application of lactobacillus paracasei fermented feed |
| CN103734479A (en) * | 2014-01-10 | 2014-04-23 | 孙震海 | Production method of feed additive capable of reducing contents of estrogen and cholesterol in bodies of livestock and poultry |
| CN104012754A (en) * | 2014-06-10 | 2014-09-03 | 寿阳华阳农牧有限公司 | Feed rich in lactococcus lactis and preparation method thereof |
| CN104012758A (en) * | 2014-06-19 | 2014-09-03 | 米易县万民农牧有限责任公司 | Biological fermentation feed and preparation method thereof |
| CN104106727A (en) * | 2014-06-12 | 2014-10-22 | 吉林省农业科学院 | Complete fermented feed and preparing method thereof |
| CN105166385A (en) * | 2015-09-23 | 2015-12-23 | 安徽省兴源草业有限公司 | Fermented feed with ragimillet seeds and fermenting agent |
| CN105285471A (en) * | 2015-11-20 | 2016-02-03 | 刘惠强 | Broiler chicken feed and preparation method thereof |
| CN105394333A (en) * | 2015-09-29 | 2016-03-16 | 何建国 | Microorganism-containing element for replacing antibiotics and hormones in breeding |
| CN106490376A (en) * | 2016-10-09 | 2017-03-15 | 赵纯伟 | Fodder compound of six black Gallus Domesticuss and its preparation method and application |
| CN106889307A (en) * | 2015-12-18 | 2017-06-27 | 顾国忠 | A kind of production technology of biological feedstuff of stalk |
| WO2017178514A1 (en) * | 2016-04-13 | 2017-10-19 | Dupont Nutrition Biosciences Aps | Manufacture of a cereal-based lactic acid-fermented product |
| CN107929329A (en) * | 2017-07-07 | 2018-04-20 | 西北民族大学 | A kind of prebiotic compound bacteria Chinese medicinal granule of embolism extinguishing lipid-loweringing and preparation method thereof |
| CN108018317A (en) * | 2017-12-18 | 2018-05-11 | 江南大学 | A kind of method for improving Lactococcus lactis resistance and the application in bacterium powder preparation |
| CN109207389A (en) * | 2017-07-07 | 2019-01-15 | 西北民族大学 | A kind of prebiotic compound bacteria traditional Chinese medicine oral liquid of embolism extinguishing lipid-loweringing and preparation method thereof |
| PL422603A1 (en) * | 2017-08-21 | 2019-02-25 | Politechnika Łódzka | Lactic bacterial strain of Lactobacillus paracasei |
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| US4985246A (en) * | 1985-10-31 | 1991-01-15 | Morinaga Milk Industry Co., Ltd. | Composition of feedstuff |
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| US4985246A (en) * | 1985-10-31 | 1991-01-15 | Morinaga Milk Industry Co., Ltd. | Composition of feedstuff |
| CN1117803A (en) * | 1994-08-29 | 1996-03-06 | 黄殿鹏 | Novel feed (probiotics) and production process thereof |
Cited By (22)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102994420A (en) * | 2012-11-09 | 2013-03-27 | 黑龙江八一农垦大学 | Low-temperature resistant lactobacillus with ACE (angiotensin converting enzyme) inhibitory activity |
| CN102994420B (en) * | 2012-11-09 | 2014-08-20 | 黑龙江八一农垦大学 | Low-temperature resistant lactobacillus with ACE (angiotensin converting enzyme) inhibitory activity |
| CN102987435A (en) * | 2012-11-13 | 2013-03-27 | 西华大学 | Preparation method for multifunctional complex-fermented powder for tenderization and fermentation and method for preparing fermented yak meat particles |
| CN103070287A (en) * | 2013-02-26 | 2013-05-01 | 湖南省微生物研究所 | Lactobacillus paracasei fermented feed and preparation method thereof, fermenting equipment and application of lactobacillus paracasei fermented feed |
| CN103734479B (en) * | 2014-01-10 | 2015-04-22 | 孙震海 | Production method of feed additive capable of reducing contents of estrogen and cholesterol in bodies of livestock and poultry |
| CN103734479A (en) * | 2014-01-10 | 2014-04-23 | 孙震海 | Production method of feed additive capable of reducing contents of estrogen and cholesterol in bodies of livestock and poultry |
| CN104012754A (en) * | 2014-06-10 | 2014-09-03 | 寿阳华阳农牧有限公司 | Feed rich in lactococcus lactis and preparation method thereof |
| CN104106727A (en) * | 2014-06-12 | 2014-10-22 | 吉林省农业科学院 | Complete fermented feed and preparing method thereof |
| CN104012758B (en) * | 2014-06-19 | 2016-01-13 | 米易县万民农牧有限责任公司 | biological fermentation feed and preparation method thereof |
| CN104012758A (en) * | 2014-06-19 | 2014-09-03 | 米易县万民农牧有限责任公司 | Biological fermentation feed and preparation method thereof |
| CN105166385A (en) * | 2015-09-23 | 2015-12-23 | 安徽省兴源草业有限公司 | Fermented feed with ragimillet seeds and fermenting agent |
| CN105394333A (en) * | 2015-09-29 | 2016-03-16 | 何建国 | Microorganism-containing element for replacing antibiotics and hormones in breeding |
| CN105285471A (en) * | 2015-11-20 | 2016-02-03 | 刘惠强 | Broiler chicken feed and preparation method thereof |
| CN106889307A (en) * | 2015-12-18 | 2017-06-27 | 顾国忠 | A kind of production technology of biological feedstuff of stalk |
| WO2017178514A1 (en) * | 2016-04-13 | 2017-10-19 | Dupont Nutrition Biosciences Aps | Manufacture of a cereal-based lactic acid-fermented product |
| CN109068675A (en) * | 2016-04-13 | 2018-12-21 | 杜邦营养生物科学有限公司 | The manufacture of cereal-based lactic acid fermented prod |
| US11470864B2 (en) | 2016-04-13 | 2022-10-18 | Dupont Nutrition Biosciences Aps | Manufacture of a cereal-based lactic acid-fermented product |
| CN106490376A (en) * | 2016-10-09 | 2017-03-15 | 赵纯伟 | Fodder compound of six black Gallus Domesticuss and its preparation method and application |
| CN107929329A (en) * | 2017-07-07 | 2018-04-20 | 西北民族大学 | A kind of prebiotic compound bacteria Chinese medicinal granule of embolism extinguishing lipid-loweringing and preparation method thereof |
| CN109207389A (en) * | 2017-07-07 | 2019-01-15 | 西北民族大学 | A kind of prebiotic compound bacteria traditional Chinese medicine oral liquid of embolism extinguishing lipid-loweringing and preparation method thereof |
| PL422603A1 (en) * | 2017-08-21 | 2019-02-25 | Politechnika Łódzka | Lactic bacterial strain of Lactobacillus paracasei |
| CN108018317A (en) * | 2017-12-18 | 2018-05-11 | 江南大学 | A kind of method for improving Lactococcus lactis resistance and the application in bacterium powder preparation |
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