CN102408280A - High-activity antibacterial peptide-containing matrix for garden seedling cultivation, as well as preparation and application thereof - Google Patents
High-activity antibacterial peptide-containing matrix for garden seedling cultivation, as well as preparation and application thereof Download PDFInfo
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Abstract
The invention discloses a high-activity antibacterial peptide-containing matrix for garden seedling cultivation, as well as a preparation method and application thereof. The high-activity antibacterial peptide-containing matrix for garden seedling cultivation is prepared from the following components: Chinese medicament residues, organic additives, inorganic additives, microbial blends, housefly larva excrement, functional microbes and a water retention agent, wherein the functional microbes have the effects of activating matrix nutrition, exogenously fixing nitrogen, antagonizing pathogenic microorganism, reinforcing absorbing capability of seedling roots to nutrition and promoting seedling growth. Furthermore, the matrix for garden seedling cultivation has comprehensive nutrient elements, stable performance, strong buffer capability, and good water and fertilizer retaining performance; and the preparation method has simple production process, short production period, wide sources and low cost of raw materials, no environmental pollution and convenience in large-scale commercial production. The matrix for garden seedling cultivation can be used for raising and cultivating garden green seedlings, and can also be used for repairing and improving the ecological environment.
Description
Technical field
The invention belongs to agriculture field, be specifically related to a kind of garden seedling tree cultivation and use matrix, particularly a kind of garden seedling tree cultivation that contains the high reactivity antibacterial peptide is with matrix and preparation method thereof and application.
Background technology
The garden seedling tree culture substrate is the basis and the media of seedling growth; Except support, fixedly the nursery stock plant; The more important thing is effect with transhipment station; Make nutrient, moisture from nutritive medium be able to transfer, root system of plant is therefrom selected to absorb as required, and good subenvironment can be provided for the growth of container seedling.Especially the beneficial microbe colony in the matrix; Can be except that self containing nutritive elements such as a certain amount of C, N, P by the plant absorbing utilization; Also can carry out nitrification, ammonification etc. through metabolic processes; Be not merely plant good nutritional condition is provided, but also quickened the circulation of nutritive element in the matrix microecosystem; Resolvent after this epimatrix mikrobe self meta-bolites and the death can be by the plant absorbing utilization, and some meta-bolitess of plant (like root secretion) or cast also can be absorbed by the matrix mikrobe; Formed one " partial circulating " in matrix microecosystem inside thus; Make and form the stable dynamic system between matrix mikrobe and root system of plant; The root system of plant provides the part carbon source for the matrix mikrobe in this system, and the matrix mikrobe also promotes the growth of root system of plant conversely.Than soil media, the particle of culture substrate is bigger, and air porosity is higher, can help seedling growth more, and the culture substrate of equal volume is compared with soil media, and is all superior aspect many at nutrition, moisture etc.
Soilless culture substrate mainly is divided into inorganic and organic two big types at present, and general inorganic type soilless culture substrate is rock wool, perlite, vermiculite etc. both at home and abroad, and organic substrate for soilless culture is a peat.But the former is difficult to degraded at nature; Become solid waste behind the raise crop; Cause environmental pollution; The utilization of latter's mass development not only causes the irreversible destruction of earth Wetlands ecosystems, also causes the peat resource to exhaust day by day, has had many countries to begin to limit the exploitation and the use of these several types of matrix.
The surrogate of peat and rock wool is used sawdust like Canada so countries in the world begin one's study, and Israel is with cow dung and trester, and Britain all obtains good result with coir etc.China Liu Qing China etc. is studied the later organic waste that ferments (saw dust, coconut palm chaff, beans stalk powder, peanut hull meal, corn cob meal, vinasse) environment-friendly type matrix from the angle of resiliency; The result shows that each environment-friendly substrate acid, alkali, salt buffer capacity all are higher than peat and other inorganic matrix; Can form metastable rhizospheric environment, at utmost prevent because the acute variation of nutritive medium pH value or EC value damages plant root.
CN102100170A provides a kind of method of utilizing rice wheat straw to make plantation matrix, has promptly solved environmental issue, has made full use of the surplus value of rice wheat straw again; But it is not enough to plant the matrix rotten degree, be prone to cause the burning seedling, and operation is more loaded down with trivial details, and use cost is higher.It is the botanical seedling culturing of raw material and the matrix and the method for manufacture thereof of cultivation usefulness that CN 1394469A provides a kind of being pickled with grains or in wine with vinegar, and its advantage is to turn waste into wealth, and does not cause environmental pollution; Cost is low, reduces more than 60% than the peat composed of rotten mosses; But there is the composting time long (need 30～40 days summer approximately, and need 45～55 days winter approximately) in it, and the composting time receives seasonal effect obvious, only in the composting process, adds the bacterial classification mixtinite that helps becoming thoroughly decomposed, its functional microbial limited amount.
Summary of the invention
For the shortcoming and deficiency that overcomes prior art, primary and foremost purpose of the present invention is to provide a kind of garden seedling tree cultivation that contains the high reactivity antibacterial peptide to use matrix.
Another object of the present invention is to provide the above-mentioned preparation method who contains the garden seedling tree cultivation of high reactivity antibacterial peptide with matrix; This matrix is to develop according to the growth rhythm of most of Nursery Stock with to the characteristics of demand of matrix physicochemical character; Be with Chinese medicine slag, organic additive, the inorganic additives laggard aerobe fermentation compost of acting charitably of proportioning by a certain percentage; Change Cheng Huacang over to after the aerobic fermentation compost is accomplished, add the fly maggot excrement again, functional microbial carries out Secondary Fermentation and forms.
A purpose more of the present invention is to provide the above-mentioned purposes that contains the garden seedling tree cultivation of high reactivity antibacterial peptide with matrix.
The object of the invention is realized through following technical proposals:
Matrix is used in a kind of garden seedling tree cultivation that contains the high reactivity antibacterial peptide, is that the composition by following mass percent prepares:
Chinese medicine slag: 55～75%
Organic additive: 15～26%
Inorganic additives: 6～12%
Microorganism-decomposing agent: 0.4～3%
Fly maggot excrement: 2～4%
Functional microbial: 0.5～2%
Water-holding agent: 0.2～2%;
Described Chinese medicine slag is the solid waste that produces in the production processes such as herbal medicine, Chinese patent medicine, cold tea, as: the remaining Loquat Leaf and the dregs of a decoction thereof, the root of large-flowered skullcap dregs of a decoction, the shizandra berry dregs of a decoction, other Chinese medicine slags (staple is Radix Codonopsis, hawthorn, dried orange peel etc.) after glycyrrhiza residue, the Root of Indigowoad dregs of a decoction, Distillate Folium Eriobotryae and loquat leaf extract application soxhlet extraction extract;
Described organic additive is more than one in mushroom residue, stalk, wood chip, soy sauce slag, cow dung, humic acids, yeast waste liquor, furfural dregs, vinasse, acid-sludge, soya-bean cake, residue from beans after making, bone meal, bagasse, molasses or the ammonium humate;
Described inorganic additives is more than one in flyash, soda-manufacturing waste residue, ground phosphate rock, peat soil, the porous peat composed of rotten mosses, zeolite, zeyssatite or the carbon ammonium;
Described microorganism-decomposing agent is become thoroughly decomposed in agent (legendary god of farming of BeiJing, China adopts the production of standing grain bio tech ltd), enzymatic microorganism quick rotting agent (the big magnificent biological factory production in Huai) or the compound bio starter (the green bright and beautiful bio tech ltd production of Nanjing the earth) more than one of organic material composting agent (production of the open-minded biotechnology in Hubei ltd), biological fermentation;
The solid waste of described fly maggot excrement for producing in the process of culturing fly maggot;
Described functional microbial is more than one in mycorrhizal fungi (Glomus mosseae Glomus mosseae, the mould Glomus intraradices of interior sacculus), vinelandii (blown-ball Azotobacter Azotobacter chroococcum, Brasil diazotrophic spirillum Azospirillum brasilense) or the actinomycetes (streptomyces microflavus Streptomyces microflavus, Jingyang streptomycete Streptomyces jingyangensisn);
Above-mentioned mikrobe all be disclose public.
Glomus mosseae (Glomus mosseae) is optional from being preserved in CGMCC (Chinese common micro-organisms preservation administrative center), be numbered No.3451 or 0921 in a strain more than; Or be selected from and be preserved in BGC (bush mycorrhizal fungi germplasm resource bank), be numbered No.BGC XJ02, BGC BJ01, BGC HK01 is more than the strain among BGC JX01 or the BGC GD01A.
Interior sacculus mould (Glomus intraradices) is optional from the bacterial strain that is preserved in ATCC (American Type Culture Collecti).
Blown-ball Azotobacter (Azotobacter chroococcum) is optional from being preserved in ACCC (Chinese agriculture microbial strains preservation administrative center), is numbered No.10001, more than the strain in 10006,10097,11104,10105 or 01077; Or be selected from and be preserved in CICC (Chinese industrial microbial strains preservation administrative center), be numbered No.21686, more than the strain in 21685,10309,20603 or 20025.
Brasil diazotrophic spirillum (Azospirillum brasilense) is optional from being preserved in ACCC, is numbered No.10102, more than the strain in 10104,10100,10109 or 04138.
Streptomyces microflavus (Streptomyces microflavus) is optional from being preserved in ACCC, is numbered No.40027, more than the strain in 41134,40776,40802 or 40906; Or be selected from and be preserved in CICC, be numbered No.11006, more than the strain in 23627 or 23626.
Jingyang streptomycete (Streptomyces jingyangensisn) is optional from being preserved in ACCC, is numbered No.40021, more than the strain in 40040,40058,40084,40126 or 40086; Or be selected from and be preserved in CICC, be numbered No.11006, more than the strain in 23627 or 23626.
Said water-holding agent is acrylamide-acrylicacid salt crosslinking copolymerization thing (SEPIGEL 305, ZX-I, polyacrylic acid potassium, ammonium polyacrylate) and/or starch grafted acrylate crosslinking copolymerization thing.
The above-mentioned garden seedling tree cultivation that contains the high reactivity antibacterial peptide is as shown in the table with the relevant performance perameter of matrix:
The above-mentioned garden seedling tree cultivation that contains the high reactivity antibacterial peptide may further comprise the steps with the preparation method of matrix:
(1) according to compost parameter p H value 6～8.5, C/N ratio (C/N) (mass ratio) 20～35: 1, organic quality percentage composition is 45～60%; Chinese medicine slag with 55～75%, 15～26% organic additive, 6～12% inorganic additives and 0.4～3% microorganism-decomposing agent mix the back aerobic compost; After 6-15 days, obtain the product of fermentation for the first time;
(2) change the storage bin (Chinese patent 200610122219.4) of the hot a heatable brick bed system design of employing after for the first time the product of fermentation and 2～6% fly maggot excrement, 0.5～3% functional microbial and 0.2～2% water-holding agent mix over to; Storage bin ground plate temperature keeps 35～50 ℃, carries out Secondary Fermentation; Whenever once, when the compost material water cut reaches 10～18%, (need 12-20 days), stop fermentation at a distance from turning in 2～6 days;
(3) material of accomplishing after fermenting is for the second time pulverized and sub-sieve, gets the part of particle diameter≤5.0mm, promptly gets the garden seedling tree cultivation that contains the high reactivity antibacterial peptide and uses matrix;
The above-mentioned garden seedling tree cultivation that contains the high reactivity antibacterial peptide can be widely used in growing seedlings and cultivating of all kinds of Nursery Stock with matrix.
Chinese medicine slag is the solid waste that the tcm-industry produces.According to statistics, China only plant medicine slag annual emissions just up to more than 650,000 tons.Chinese medicine slag also contains a certain amount of activeconstituents and a large amount of robust fibre, crude fat, starch, Crude polysaccharides, amino acid etc. except that containing nutritive elements such as the required nitrogen of plant-growth, phosphorus, potassium.Chinese medicine slag contains certain nutritive ingredient and general water cut is higher, and is very easily corrupt, can cause serious pollution to environment.Early stage Chinese medicine slag forms of treatment mainly is landfill, burning, FX stacking etc., not only exhausts a large amount of funds, and has caused the waste of resource and serious environmental to pollute.
The fly maggot excrement is to culture the solid waste that produces in the fly maggot process, contains multiple antibiotic, antiviral polypeptides material.Wherein contain a kind of activated protein of extremely strong sterilizing power, if having ten thousand/ concentration, can make various pathogenic bacterias dead, be called by the organic sphere personage by " biological nuclear bomb ".Untie the method that the potted plant control test of application and culture medium culturing method combine such as control; The foster maggot product of research shows that to the antibacterial disease resisting effect of balsam pear blight pathogenic bacteria the fly maggot tissue smashs liquid to pieces and maggot excrement vat liquor has significant inhibitory effect to pathogenic bacteria mycelial growth and living weight.
With the Technology of above-mentioned two types of materials through modern advanced person; Innovation is produced and is contained antibacterial peptide garden seedling tree cultivation and use high-activity biological matrix; Can remedy the deficiency of existing stock growing matrix species, also can turn waste into wealth energy efficiency; Develop a circular economy, realize the organic unity of ecological benefits and economic benefit.
The present invention has following advantage and effect with respect to prior art:
(1) the garden seedling tree cultivation that contains the high reactivity antibacterial peptide of the present invention with matrix have nutritive element comprehensively, stable performance, advantages such as surge capability is strong, the preserve moisture and fertility performance is good, production technique is simple, with short production cycle, less investment, technological easy master.
(2) matrix use in the garden seedling tree cultivation that contains the high reactivity antibacterial peptide of the present invention, its preparation raw material wide material sources, cheap, environmentally safe, is convenient to mass-producing commercialization production.
(3) the garden seedling tree cultivation that contains the high reactivity antibacterial peptide of the present invention has activated substrate nutrient, external source fixed nitrogen, antagonism pathogenic micro-organism, strengthens the effect of seedling root to nutrient absorbing ability, promotion seedling growth with the functional microbial that contains in the matrix.
(4) the garden seedling tree cultivation that contains the high reactivity antibacterial peptide of the present invention is with the agricultural safety of matrix; Various active material that contains such as peptide material have stronger bacteriostatic and disease prevention effect; Effectively reduce the fertilising spraying times; Minimizing helps practicing thrift cost to the pollution of environment, promotes growing up strong and sturdy of nursery stock.
(5) garden seedling tree that contains the high reactivity antibacterial peptide of the present invention is cultivated the handling problem that has not only solved organic waste with matrix, also for the garden seedling tree cultivation provides the fine culture substrate, has improved the level of comprehensive utilization of natural resources.
Description of drawings
Fig. 1 is the preparing method's schematic flow sheet that contains the garden seedling tree cultivation of high reactivity antibacterial peptide with matrix of the present invention.
Embodiment
Below in conjunction with embodiment and accompanying drawing the present invention is described in further detail, but embodiment of the present invention is not limited thereto.
Embodiment 1
Contain the preparation method of the garden seedling tree cultivation of high reactivity antibacterial peptide, may further comprise the steps (as shown in Figure 1) with matrix:
(1) (the open-minded biotechnology in Hubei ltd produces with the Chinese medicine slag (being made up of glycyrrhiza residue, the Root of Indigowoad dregs of a decoction, the yellow Cen dregs of a decoction and the shizandra berry dregs of a decoction) of 7300kg, the mushroom residue of 1500kg, the flyash of 600kg and the organic material composting agent of 100kg; Mix down together), gained mixture C/N ratio (C/N) (mass ratio) 25: 1, pH 7.0, and organic quality percentage composition is 55%.Regulate water cut to 55% back aerobic compost, after 8 days, obtain the product of fermentation for the first time;
(middle section Anhui China brilliant novel material company produces the product that (2) will ferment for the first time with the fly maggot excrement of 200kg, the Glomus mosseae Glomus mosseae (CGMCC No.3451) of 150kg and the acrylamide-acrylicacid salt crosslinking copolymerization thing of 150kg again; Down with) change the storage bin (Chinese patent 200610122219.4) that adopts hot a heatable brick bed system design over to after mixing, Secondary Fermentation is carried out in 38 ℃ of storage bin ground plate temperature maintenances; Whenever once, when the compost material water cut reaches 15%, stop fermentation at a distance from turning in 4 days;
(3) material of accomplishing after fermenting for the second time carries out product sub-sieve (Φ 5.0mm) through conveying belt input crushing and classification sieve.Get the part of particle diameter≤5.0mm, promptly get the garden seedling tree cultivation that contains the high reactivity antibacterial peptide and use matrix.
The prepared garden seedling tree cultivation that contains the high reactivity antibacterial peptide that obtains of present embodiment is as shown in the table with the relevant performance perameter of matrix:
Embodiment 2
Contain the preparation method of the garden seedling tree cultivation of high reactivity antibacterial peptide, may further comprise the steps (as shown in Figure 1) with matrix:
(1) with the Chinese medicine slag (forming) of 6000kg, the organic additive (forming) of 2100kg, the inorganic additives (forming) of 1200kg and compound bio starter (the green bright and beautiful bio tech ltd production of Nanjing the earth of 230kg by soda-manufacturing waste residue and ground phosphate rock by stalk, wood chip, soy sauce slag, cow dung and humic acids by glycyrrhiza residue, the Root of Indigowoad dregs of a decoction, the yellow Cen dregs of a decoction and the shizandra berry dregs of a decoction; Mix down together), gained mixture C/N ratio (C/N) (mass ratio) 28: 1, pH 8.0, and organic quality percentage composition is 50%.Regulate water cut to 52% back aerobic compost, after 12 days, obtain the product of fermentation for the first time;
(2) for the first time the product of fermentation (peace of the Guangzhou agriculture ltd of peace letter water conservation production is believed the high reactivity water-holding agent with the starch grafted acrylate crosslinking copolymerization thing of the fly maggot excrement of 400kg, the functional microbial of 50kg (being made up of Glomus mosseae Glomus mosseae (CGMCC No.3451) and blown-ball Azotobacter Azotobacter chroococcum (ACCC No.10001)) and 20kg again; Down with) change the storage bin (Chinese patent 200610122219.4) that adopts hot a heatable brick bed system design over to after mixing, Secondary Fermentation is carried out in 50 ℃ of storage bin ground plate temperature maintenances; Whenever once, when the compost material water cut reaches 18%, stop fermentation at a distance from turning in 2 days;
(3) material of accomplishing after fermenting for the second time carries out product sub-sieve (Φ 5.0mm) through conveying belt input crushing and classification sieve.Get the part of particle diameter≤5.0mm, promptly get the garden seedling tree cultivation that contains the high reactivity antibacterial peptide and use matrix.
The prepared garden seedling tree cultivation that contains the high reactivity antibacterial peptide that obtains of present embodiment is as shown in the table with the relevant performance perameter of matrix:
Embodiment 3
Contain the preparation method of the garden seedling tree cultivation of high reactivity antibacterial peptide, may further comprise the steps (as shown in Figure 1) with matrix:
(1) (being become thoroughly decomposed by organic material composting agent and biological fermentation, (legendary god of farming of BeiJing, China adopts the production of standing grain bio tech ltd in agent with the inorganic additives (being made up of the peat soil and the porous peat composed of rotten mosses) of the organic additive (being made up of yeast waste liquor, furfural dregs, vinasse, acid-sludge and soya-bean cake) of the Chinese medicine slag (being made up of glycyrrhiza residue, the Root of Indigowoad dregs of a decoction, the yellow Cen dregs of a decoction and the shizandra berry dregs of a decoction) of 7500kg, 1500kg, 600kg and the microorganism-decomposing agent of 40kg; Form down together)) mix, gained mixture C/N ratio (C/N) (mass ratio) 20: 1, pH 6, and organic quality percentage composition is 45%.Regulate water cut to 48% back aerobic compost, after 6 days, obtain the product of fermentation for the first time;
(2) change the storage bin (Chinese patent 200610122219.4) of the hot a heatable brick bed system design of employing after for the first time the product of fermentation mixes with the water-holding agent (being made up of acrylamide-acrylicacid salt crosslinking copolymerization thing and starch grafted acrylate crosslinking copolymerization thing) of the fly maggot excrement of 200kg, the functional microbial of 170kg (being made up of the mould Glomus intraradices of interior sacculus and streptomyces microflavus Streptomyces microflavus (CICCNo.11006)) and 50kg again over to; Storage bin ground plate temperature keeps 45 ℃, carries out Secondary Fermentation; Whenever once, when the compost material water cut reaches 16%, stop fermentation at a distance from turning in 3 days;
(3) material of accomplishing after fermenting for the second time carries out product sub-sieve (Φ 5.0mm) through conveying belt input crushing and classification sieve.Get the part of particle diameter≤5.0mm, promptly get the garden seedling tree cultivation that contains the high reactivity antibacterial peptide and use matrix.
The prepared garden seedling tree cultivation that contains the high reactivity antibacterial peptide that obtains of present embodiment is as shown in the table with the relevant performance perameter of matrix:
Embodiment 4
Contain the preparation method of the garden seedling tree cultivation of high reactivity antibacterial peptide, may further comprise the steps (as shown in Figure 1) with matrix:
(1) Chinese medicine slag (being made up of glycyrrhiza residue, the Root of Indigowoad dregs of a decoction, the yellow Cen dregs of a decoction and the shizandra berry dregs of a decoction) of 5500kg, the organic additive (being made up of residue from beans after making, bone meal, bagasse, molasses and ammonium humate) of 2600kg, the inorganic additives (being made up of zeolite, zeyssatite and carbon ammonium) of 1000kg and the microorganism-decomposing agent (being made up of enzymatic microorganism quick rotting agent (the big magnificent biological factory production in Huai) and compound bio starter) of 300kg are mixed; Gained mixture C/N ratio (C/N) (mass ratio) 35: 1; PH 8.5, and organic quality percentage composition is 60%.Regulate water cut to 60% back aerobic compost, after 15 days, obtain the product of fermentation for the first time;
(2) change the storage bin (Chinese patent 200610122219.4) of the hot a heatable brick bed system design of employing after for the first time the product of fermentation mixes with the water-holding agent (being made up of acrylamide-acrylicacid salt crosslinking copolymerization thing and starch grafted acrylate crosslinking copolymerization thing) of the fly maggot excrement of 200kg, the functional microbial of 200kg (being made up of Brasil diazotrophic spirillum Azospirillum brasilense (ACCC No.10102) and Jingyang streptomycete Streptomyces jingyangensisn (ACCC No.40021)) and 200kg again over to; Storage bin ground plate temperature keeps 35 ℃, carries out Secondary Fermentation; Whenever once, when the compost material water cut reaches 10%, stop fermentation at a distance from turning in 6 days;
(3) material of accomplishing after fermenting for the second time carries out product sub-sieve (Φ 5.0mm) through conveying belt input crushing and classification sieve.Get the part of particle diameter≤5.0mm, promptly get the garden seedling tree cultivation that contains the high reactivity antibacterial peptide and use matrix.
The prepared garden seedling tree cultivation that contains the high reactivity antibacterial peptide that obtains of present embodiment is as shown in the table with the relevant performance perameter of matrix:
The effect of above embodiment 1～4 and Comparative Examples is following:
Make Comparative Examples with organic-inorganic mixed-matrix (peat, perlite, vermiculite three's mass ratio is 2: 1: 1), carry out the effect test of above-mentioned instance product in Inst. of Soil & Fertilizer, Guangdong Prov. Academy of Agricultural Science achievements conversion test base.Test period is on December 30,2010 26 days to 2010 February in.Supplying the examination nursery stock is the four seasons osmanthus that production and sales are bigger on the market (Osmanthus fragrans var..semperflorens).
The result is as shown in table 1:
Table 1 is compared the plant height and the performance of stem diameter in embodiment matrix four seasons osmanthus with Comparative Examples
Annotate: the stem diameter refers to liftoff 10cm place.
When measuring on December 30th, 2010, four kinds of embodiment matrix four seasons osmanthus plant height increasing amounts are 42.3～46.1cm, and stem increasing diameter dosage is 0.30～0.38cm, and average specific Comparative Examples plant height increases 11.0cm, and the stem diameter increases 0.12cm.Illustrative embodiment matrix can better promote the growth of four seasons osmanthus plant height, stem diameter.
The foregoing description is a preferred implementation of the present invention; But embodiment of the present invention is not restricted to the described embodiments; Other any do not deviate from change, the modification done under spirit of the present invention and the principle, substitutes, combination, simplify; All should be the substitute mode of equivalence, be included within protection scope of the present invention.
Claims (8)
1. matrix is used in a garden seedling tree cultivation that contains the high reactivity antibacterial peptide, it is characterized in that it being that composition by following mass percent prepares:
Chinese medicine slag: 55～75%
Organic additive: 15～26%
Inorganic additives: 6～12%
Microorganism-decomposing agent: 0.4～3%
Fly maggot excrement: 2～4%
Functional microbial: 0.5～2%
Water-holding agent: 0.2～2%;
Described functional microbial is that Glomus mosseae, interior sacculus are mould, in azospirillum, Brasil diazotrophic spirillum, streptomyces microflavus or the Jingyang streptomycete more than one.
2. matrix is used in the garden seedling tree cultivation that contains the high reactivity antibacterial peptide according to claim 1, and it is characterized in that: described Chinese medicine slag is the two or more mixtures in glycyrrhiza residue, the Root of Indigowoad dregs of a decoction, the yellow Cen dregs of a decoction or the shizandra berry dregs of a decoction.
3. matrix is used in the garden seedling tree cultivation that contains the high reactivity antibacterial peptide according to claim 1, it is characterized in that: described microorganism-decomposing agent is become thoroughly decomposed in agent, enzymatic microorganism quick rotting agent or the compound bio starter more than one of organic material composting agent, biological fermentation.
4. matrix is used in the garden seedling tree cultivation that contains the high reactivity antibacterial peptide according to claim 1, and it is characterized in that: described organic additive is more than one in mushroom residue, stalk, wood chip, soy sauce slag, cow dung, humic acids, yeast waste liquor, furfural dregs, vinasse, acid-sludge, soya-bean cake, residue from beans after making, bone meal, bagasse, molasses or the ammonium humate.
5. matrix is used in the garden seedling tree cultivation that contains the high reactivity antibacterial peptide according to claim 1, and it is characterized in that: described inorganic additives is more than one in flyash, soda-manufacturing waste residue, ground phosphate rock, peat soil, the porous peat composed of rotten mosses, zeolite, zeyssatite or the carbon ammonium.
6. matrix is used in the garden seedling tree cultivation that contains the high reactivity antibacterial peptide according to claim 1, and it is characterized in that: described water-holding agent is acrylamide-acrylicacid salt crosslinking copolymerization thing and/or starch grafted acrylate crosslinking copolymerization thing.
7. each described garden seedling tree that contains the high reactivity antibacterial peptide of claim 1-6 is cultivated the preparation method with matrix, it is characterized in that may further comprise the steps:
(1) according to compost parameter p H value 6～8.5, carbon nitrogen mass ratio 20～35: 1, organic quality percentage composition is 45～60%; Chinese medicine slag, organic additive, inorganic additives and microorganism-decomposing agent are mixed the back aerobic compost; After 6-15 days, obtain the product of fermentation for the first time;
(2) change storage bin over to after the product of fermentation and fly maggot excrement, functional microbial and water-holding agent mix for the first time, storage bin ground plate temperature keeps 35～50 ℃, carries out Secondary Fermentation; Whenever once, when the compost material water cut reaches 10～18%, stop fermentation at a distance from turning in 2～6 days;
(3) material of accomplishing after fermenting is for the second time pulverized and sub-sieve, gets the part of particle diameter≤5.0mm, promptly gets the garden seedling tree cultivation that contains the high reactivity antibacterial peptide and uses matrix.
8. each described garden seedling tree cultivation that contains the high reactivity antibacterial peptide of claim 1-6 is used for growing seedlings and cultivating of Nursery Stock with matrix.
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