CN102319284A - Method for extracting isoflavone from kudzuvine root efficiently - Google Patents
Method for extracting isoflavone from kudzuvine root efficiently Download PDFInfo
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- CN102319284A CN102319284A CN201110199450A CN201110199450A CN102319284A CN 102319284 A CN102319284 A CN 102319284A CN 201110199450 A CN201110199450 A CN 201110199450A CN 201110199450 A CN201110199450 A CN 201110199450A CN 102319284 A CN102319284 A CN 102319284A
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- radix puerariae
- isoflavone
- kudzuvine root
- enzymolysis
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Abstract
The invention discloses a method for extracting isoflavone from kudzuvine root efficiently, and belongs to the technical field of the processing of agricultural products and food. The method comprises the following steps of: (1) crushing, namely crushing kudzuvine root to form 60 to 80-mesh kudzuvine root powder; (2) performing enzymolysis, namely adding 70 percent of ethanol into the kudzuvine root powder, adding cellulase and protease and performing the enzymolysis to obtain kudzuvine root extract, wherein 1 to 2 grams of the cellulase and the protease are added into each 100 milliliters of solution; and (3) extracting, namely performing ultrasonic-microwave processing on the obtained kudzuvine root extract from the step (2), concentrating under reduced pressure, freezing and drying to obtain kudzuvine root isoflavone. In the method, the kudzuvine root isoflavone is extracted by a compound enzymolysis technology of the cellulase and the protease and combining an ultrasonic-microwave assisted extraction technology, so the technical advantages of chemical enzymolysis and physical extraction are expressed fully, the cost is reduced, and the dissolution rate and extraction rate of the kudzuvine root isoflavone are improved, so that the extraction purity is greatly higher than that by conventional extraction method.
Description
Technical field
The present invention relates to a kind of method of from plant, extracting chemical compound, relate in particular to a kind of from Radix Puerariae high efficiency extraction prepare the method for isoflavone.
Background technology
The extraction of isoflavone and effect receive much concern in recent years; The method of report extraction Radix Puerariae isoflavone from Radix Puerariae has at present: heating reflux method, percolation, infusion process, physical strengthening assisted extraction etc.; Wherein the circumfluence method effective component extraction rate is higher; But solvent load is big, and cost is high, discarded liquid contaminated environment; The percolation solvent for use is less, but extraction time is long.Production is at present gone up extraction Radix Puerariae total isoflavone and is generally adopted hot dipping to put forward mode, but exists energy consumption big, and the time is long, inefficient shortcoming.Water extraction and organic solvent extraction are adopted in the extraction of Radix Puerariae flavone usually, adopt the lixiviate organic solvent to mainly contain ethanol, methanol etc., and pure method is to adopt ethanol or methanol to carry out hot reflux to extract, and precipitates with lead acetate.Water law is to adopt decocting to boil, and uses Acid precipitation.Alcohol extracting method gained purity is higher, but process is loaded down with trivial details, loss of effective components is many, extraction ratio is low, the time is long and cost is high, is mainly used in medicine and clinical purpose.Water extraction exists purity, shortcoming that extraction ratio is lower.
In view of method for distilling such as the traditional heating reflux method of present Radix Puerariae isoflavone, percolation, infusion process exist energy consumption big, the time is long, and inefficient shortcoming should not be used for commercial production.But the stripping that the method useful effect that therefore combines chemical enzymolysis and physics to extract also at utmost improves cellular content at the Radix Puerariae cell wall, thereby the extraction ratio of raising Radix Puerariae isoflavone.
Summary of the invention
Goal of the invention: in order to overcome the deficiency that exists in the prior art, the present invention provides a kind of method that combines chemical enzymolysis and physics to extract and can effectively improve the isoflavone extraction ratio.
Technical scheme: for realizing above-mentioned purpose, method for distilling step of the present invention is following:
(1) pulverizes: get powder of Radix Puerariae and be broken to 60-80 purpose powder of Radix Puerariae.
(2) enzymolysis: powder of Radix Puerariae is added 70% ethanol, add cellulase and protease again and carry out enzymolysis and obtain Radix Puerariae extracting solution, it is per 100 ml soln 1-2g that said cellulase and protease add total amount.
(3) extract: the Radix Puerariae extracting solution that step (2) is obtained is through ultrasonic-microwave treatment, and concentrating under reduced pressure, lyophilization make Radix Puerariae isoflavone then.
Wherein, It is in order to have increased the alcoholic acid contact area of Radix Puerariae raw material and extraction solvent that Radix Puerariae is pulverized; Thereby for the extraction ratio that improves Radix Puerariae isoflavone lays the foundation; Adopting cellulase and protein enzyme to carry out enzymolysis is in order to quicken the stripping of cellular content, and the enzymolysis mode can pass through and reconcile the enzymolysis environment control extent of reaction, make reaction temperature with efficiently, guaranteed the dna purity and the efficient of isoflavone.
Powder of Radix Puerariae described in the step (2) and 70% alcoholic acid solid-liquid ratio (g/mL) are 1:10-35, and the mass ratio of said cellulase and protease is 1:1-3, and hydrolysis temperature is 58-63 ℃, and enzymolysis time is 56-66min, and pH value is 6.
The supersonic frequency of ultrasonic-microwave treatment is 40KHz in the step (3), microwave power 86-96W, and temperature is controlled at 45-53 ℃ and extracts 20-30min.Wherein, the ultrasonic transducer output is 40KHz.
Beneficial effect: the present invention adopts cellulase and protease complex enzyme hydrolysis technology; And combine ultrasonic-microwave auxiliary extraction technology Radix Puerariae isoflavone; Give full play to the technical advantage that chemical enzymolysis and physics extract; Reduce cost, and improved the dissolution rate and the extraction ratio of Radix Puerariae isoflavone, make dna purity be much higher than present existing method for distilling.
The specific embodiment
Embodiment 1
Get the wild Radix Puerariae 1000g in Jurong and be crushed to 60-80 purpose powder of Radix Puerariae.Powder of Radix Puerariae is added lixiviate in 70% alcoholic solution of 10L, regulating pH is 6, adds 100g cellulase and 100g protease hydrolyzeds down at 60 ℃, and enzymolysis time is 60min, obtains Radix Puerariae extracting solution.Behind the enzymolysis with Radix Puerariae extracting solution through ultrasonic-microwave treatment, the supersonic frequency of ultrasonic-microwave treatment is 40KHz, the ultrasonic transducer output is 40KHz, microwave power 86-96W, temperature is controlled at 45-53 ℃ and extracts 25min.Concentrating under reduced pressure, lyophilization make Radix Puerariae isoflavone then.
Embodiment 2
Get the wild Radix Puerariae 1000g in Jurong and be crushed to 60-80 purpose powder of Radix Puerariae.Powder of Radix Puerariae is added lixiviate in 70% alcoholic solution of 25L, regulating pH is 6, adds 125g cellulase and 250g protease hydrolyzeds down at 60 ℃, and enzymolysis time is 66min, obtains Radix Puerariae extracting solution.Behind the enzymolysis with Radix Puerariae extracting solution through ultrasonic-microwave treatment, the supersonic frequency of ultrasonic-microwave treatment is 40KHz, the ultrasonic transducer output is 40KHz, microwave power 86-96W, temperature is controlled at 45-53 ℃ and extracts 30min.Concentrating under reduced pressure, lyophilization make Radix Puerariae isoflavone then.
Embodiment 3
Get the wild Radix Puerariae 1000g in Jurong and be crushed to 60-80 purpose powder of Radix Puerariae.Powder of Radix Puerariae is added lixiviate in 70% alcoholic solution of 35L, regulating pH is 6, adds 88g cellulase and 262g protease hydrolyzeds down at 60 ℃, and enzymolysis time is 58min, obtains Radix Puerariae extracting solution.Behind the enzymolysis with Radix Puerariae extracting solution through ultrasonic-microwave treatment, the supersonic frequency of ultrasonic-microwave treatment is 40KHz, the ultrasonic transducer output is 40KHz, microwave power 86-96W, temperature is controlled at 45-53 ℃ and extracts 20min.Concentrating under reduced pressure, lyophilization make Radix Puerariae isoflavone then.
Test Example 1
Get the Radix Puerariae isoflavone that embodiment 1, embodiment 2 and embodiment 3 obtain respectively, calculate the Radix Puerariae isoflavone extraction ratio according to following formula:
Radix Puerariae isoflavone extraction ratio (%)=thick Radix Puerariae isoflavone weight (g)/1000 * 100
The result sees table 1.
Table 1 Radix Puerariae isoflavone extraction ratio
| ? | Radix Puerariae isoflavone extraction ratio (%) |
| Embodiment 1 | 65.8±0.35 |
| Embodiment 2 | 66.5±0.28 |
| Embodiment 3 | 66.8±0.15 |
| Meansigma methods | 66.4 |
Test Example 2
Accurately take by weighing puerarin reference substance 5mg, place the 25mL volumetric flask, add 70% dissolve with ethanol and be diluted to scale, shake up; Accurately draw 0.1,0.2,0.3,0.4 respectively; 0.5,0.6,0.7,0.8; 0.9 1.0 in the 10mL volumetric flask, and respectively adds 70% ethanol 1.0mL, adding distil water is diluted to scale and shakes up again.Other gets 70% ethanol 1.0mL and puts in the 10mL volumetric flask, and adding distil water is made blank to scale, measures absorbance in the 250nm wavelength, is abscissa with concentration, and absorbance is a vertical coordinate, the drawing standard curve.
Accurately take by weighing embodiment 1, embodiment 2 and embodiment 3 and extract the Radix Puerariae isoflavone sample 5mg that obtains, place the 25mL volumetric flask, add 70% dissolve with ethanol and be diluted to scale, shake up; Accurately draw 0.1,0.2,0.3,0.4 respectively; 0.5,0.6,0.7,0.8; 0.9 1.0 in the 10mL volumetric flask, and respectively adds 70% ethanol 1.0mL, adding distil water is diluted to scale and shakes up again.Other gets 70% ethanol 1.0mL and puts in the 10mL volumetric flask; Adding distil water is to scale; Make blank, measure absorbance in the 250nm wavelength, according to content of total flavone in the standard curve Equation for Calculating sample solution; And then calculate the content of Radix Puerariae isoflavone in the thick Radix Puerariae isoflavone sample, calculate Radix Puerariae isoflavone purity according to formula.
Radix Puerariae isoflavone purity (%)=Radix Puerariae isoflavone weight (mg)/5 * 100
The result sees table 2:
Table 2 Radix Puerariae isoflavone purity
| Test | Radix Puerariae isoflavone purity (%) |
| Embodiment 1 | 80.8±0.29 |
| Embodiment 2 | 80.2±0.32 |
| Embodiment 3 | 80.5±0.41 |
| Meansigma methods | 80.5 |
The extractive technique purity of existing Radix Puerariae isoflavone is generally 50 ~ 60%, and preparation method provided by the invention can obtain the Radix Puerariae isoflavone of purity more than 80%, far above prior art.
The above only is a preferred implementation of the present invention; Be noted that for those skilled in the art; Under the prerequisite that does not break away from the principle of the invention, can also make some improvement and retouching, these improvement and retouching also should be regarded as protection scope of the present invention.
Claims (3)
- One kind from Radix Puerariae high efficiency extraction prepare the method for isoflavone, it is characterized in that it may further comprise the steps:(1) pulverizes: get powder of Radix Puerariae and be broken to 60-80 purpose powder of Radix Puerariae;(2) enzymolysis: powder of Radix Puerariae is added 70% ethanol, add cellulase and protease again and carry out enzymolysis and obtain Radix Puerariae extracting solution, it is per 100 ml soln 1-2g that said cellulase and protease add total amount;(3) extract: the Radix Puerariae extracting solution that step (2) is obtained is through ultrasonic-microwave treatment, and concentrating under reduced pressure, lyophilization make Radix Puerariae isoflavone then.
- According to claim l described from Radix Puerariae high efficiency extraction prepare the method for isoflavone; It is characterized in that powder of Radix Puerariae described in the step (2) and 70% alcoholic acid solid-liquid ratio (g/mL) are 1:10-35; The mass ratio of said cellulase and protease is 1:1-3; Hydrolysis temperature is 58-63 ℃, and enzymolysis time is 56-66 min, and pH value is 6.
- According to claim l described from Radix Puerariae high efficiency extraction prepare the method for isoflavone, the supersonic frequency that it is characterized in that ultrasonic-microwave treatment in the step (3) is 40KHz, microwave power 86-96W, temperature is controlled at 45-53 ℃ and extracts 20-30min.
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Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102783647A (en) * | 2012-08-28 | 2012-11-21 | 句容茅宝葛业有限公司 | Preparation method of kudzu root isoflavone powder |
| CN103223001A (en) * | 2013-04-25 | 2013-07-31 | 徐州工程学院 | Microwave-supersonic wave cooperation method for extracting soy isoflavone in soy sauce residue |
| CN104154711A (en) * | 2014-08-25 | 2014-11-19 | 济南康众医药科技开发有限公司 | Freeze-drying method for preparing high-activity kudzu root decoction pieces |
| CN104173427A (en) * | 2014-08-25 | 2014-12-03 | 济南康众医药科技开发有限公司 | Lyophilized kudzu vine root serving as high-activity kudzu vine root decoction piece |
| CN104180615A (en) * | 2014-08-25 | 2014-12-03 | 济南康众医药科技开发有限公司 | Radix puerariae freeze-drying method |
| CN104945390A (en) * | 2014-04-24 | 2015-09-30 | 四川省绿超新农业科技有限责任公司 | Puerarin extraction process |
| CN105168326A (en) * | 2015-06-24 | 2015-12-23 | 黄红林 | Radix puerariae extract preparation method |
| CN105410647A (en) * | 2015-11-24 | 2016-03-23 | 肖天焱 | Radix puerariae and pumpkin noodles and making method thereof |
| CN105902609A (en) * | 2016-05-18 | 2016-08-31 | 江苏茅宝葛业有限公司 | Method for preparing high-purity pueraria flavone extract by utilizing wild kudzu vine roots |
| CN105994798A (en) * | 2016-05-18 | 2016-10-12 | 江苏茅宝葛业有限公司 | Method for preparing soluble tea pills from wild pueraria flavonoid extract |
| CN106348429A (en) * | 2016-08-26 | 2017-01-25 | 武汉理工大学 | Kudzuvine root resource utilization method for flavonol, starch and amino acid separation and purification |
| CN107897924A (en) * | 2017-12-04 | 2018-04-13 | 德兴市宋氏葛业有限公司 | A kind of kudzuvine root starch electuary for liver-protecting sobering up and preparation method thereof |
| CN109206393A (en) * | 2018-11-15 | 2019-01-15 | 仲恺农业工程学院 | A kind of extracting method of golden-pomelo young fruit flavone compound |
| CN115120567A (en) * | 2022-08-02 | 2022-09-30 | 信阳农林学院 | Method for improving content of effective ingredients in radix puerariae and rhizoma polygonati hypoglycemic chewable tablets |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1493695A (en) * | 2003-08-22 | 2004-05-05 | 李全宏 | Prcess of extracting kudzu vine root starch, kudiu vine root polysaccharide and kudzu vine root isoflavone from kudzu vine root synchronously |
| CN101020720A (en) * | 2006-12-28 | 2007-08-22 | 宋桃森 | Process of extracting pueraria polysaccharide |
-
2011
- 2011-07-18 CN CN201110199450A patent/CN102319284A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1493695A (en) * | 2003-08-22 | 2004-05-05 | 李全宏 | Prcess of extracting kudzu vine root starch, kudiu vine root polysaccharide and kudzu vine root isoflavone from kudzu vine root synchronously |
| CN101020720A (en) * | 2006-12-28 | 2007-08-22 | 宋桃森 | Process of extracting pueraria polysaccharide |
Non-Patent Citations (3)
| Title |
|---|
| 孙术国: "葛根总黄酮和葛根素的提取及其分离纯化研究进展", 《农业工程技术(农产品加工业)》 * |
| 杨胜丹: "超声波、微波萃取及其联用技术在中药有效成分提取中的应用", 《广东化工》 * |
| 汤海鸥: "复合酶发酵液酶解提取葛根黄酮的研究", 《饲料与畜牧》 * |
Cited By (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102783647A (en) * | 2012-08-28 | 2012-11-21 | 句容茅宝葛业有限公司 | Preparation method of kudzu root isoflavone powder |
| CN103223001A (en) * | 2013-04-25 | 2013-07-31 | 徐州工程学院 | Microwave-supersonic wave cooperation method for extracting soy isoflavone in soy sauce residue |
| CN103223001B (en) * | 2013-04-25 | 2014-10-22 | 徐州工程学院 | Microwave-supersonic wave cooperation method for extracting soy isoflavone in soy sauce residue |
| CN104945390A (en) * | 2014-04-24 | 2015-09-30 | 四川省绿超新农业科技有限责任公司 | Puerarin extraction process |
| CN104154711A (en) * | 2014-08-25 | 2014-11-19 | 济南康众医药科技开发有限公司 | Freeze-drying method for preparing high-activity kudzu root decoction pieces |
| CN104173427A (en) * | 2014-08-25 | 2014-12-03 | 济南康众医药科技开发有限公司 | Lyophilized kudzu vine root serving as high-activity kudzu vine root decoction piece |
| CN104180615A (en) * | 2014-08-25 | 2014-12-03 | 济南康众医药科技开发有限公司 | Radix puerariae freeze-drying method |
| CN105168326A (en) * | 2015-06-24 | 2015-12-23 | 黄红林 | Radix puerariae extract preparation method |
| CN105410647A (en) * | 2015-11-24 | 2016-03-23 | 肖天焱 | Radix puerariae and pumpkin noodles and making method thereof |
| CN105902609A (en) * | 2016-05-18 | 2016-08-31 | 江苏茅宝葛业有限公司 | Method for preparing high-purity pueraria flavone extract by utilizing wild kudzu vine roots |
| CN105994798A (en) * | 2016-05-18 | 2016-10-12 | 江苏茅宝葛业有限公司 | Method for preparing soluble tea pills from wild pueraria flavonoid extract |
| CN106348429A (en) * | 2016-08-26 | 2017-01-25 | 武汉理工大学 | Kudzuvine root resource utilization method for flavonol, starch and amino acid separation and purification |
| CN106348429B (en) * | 2016-08-26 | 2019-05-24 | 武汉理工大学 | A kind of pueraria lobata resource utilization method of separating-purifying flavonol, starch and amino acid |
| CN107897924A (en) * | 2017-12-04 | 2018-04-13 | 德兴市宋氏葛业有限公司 | A kind of kudzuvine root starch electuary for liver-protecting sobering up and preparation method thereof |
| CN109206393A (en) * | 2018-11-15 | 2019-01-15 | 仲恺农业工程学院 | A kind of extracting method of golden-pomelo young fruit flavone compound |
| CN115120567A (en) * | 2022-08-02 | 2022-09-30 | 信阳农林学院 | Method for improving content of effective ingredients in radix puerariae and rhizoma polygonati hypoglycemic chewable tablets |
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Application publication date: 20120118 |