CN1493695A - Prcess of extracting kudzu vine root starch, kudiu vine root polysaccharide and kudzu vine root isoflavone from kudzu vine root synchronously - Google Patents
Prcess of extracting kudzu vine root starch, kudiu vine root polysaccharide and kudzu vine root isoflavone from kudzu vine root synchronously Download PDFInfo
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- CN1493695A CN1493695A CNA031247547A CN03124754A CN1493695A CN 1493695 A CN1493695 A CN 1493695A CN A031247547 A CNA031247547 A CN A031247547A CN 03124754 A CN03124754 A CN 03124754A CN 1493695 A CN1493695 A CN 1493695A
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Abstract
A process for synchronously extracting the pueraria starch, pueraria polyose and pueraria isoflavone includes enzymolysis, depositing in alcohol, vacuum, concentrating and spray drying of microsoftgels. Its advantages are high utilization rate of raw material and no poison of products.
Description
Technical field the invention provides a kind of active ingredients of plants extraction process, the technology that particularly a kind of root of kudzu vine effective ingredient is extracted.
The background technology root of kudzu vine is the subterraneous root of Pueraria lobota platymiscium, is perennial pulse family vine.The root of kudzu vine is traditional Chinese medicine commonly used, and the traditional Chinese medical science thinks that it has expelling pathogenic factors from muscles for reducing heat, promotes the production of body fluid, examines thoroughly, rises all functions of positive antidiarrheal.Since the 1950's, modern medicine has carried out having goed deep into systematic research to the medicinal component and the pharmacological action of the root of kudzu vine both at home and abroad, outside its rich in starch, also contains multiple compounds such as isoflavones, root of kudzu vine glucoside, triterpenoid saponin, alkaloid.With Radix Puerariae extract, Chinese patent medicine such as " the Yufengningxin ball " made as Radix Puerariae isoflavone etc. and puerarin injection has been applied to clinical.Existing root of kudzu vine effective ingredient extracting method is more, adopts technical process such as lixiviate, separation, concentrate drying more.In general, existing technology pays attention to extracting single effective ingredient in the root of kudzu vine, go up " Radix Puerariae total flavones extraction process " (Wang Changzhao etc./95 of publication as " herbal medicine ", 17 (6) 6-8), Chinese patent disclosed " process for extracting medicinal component from pueraria root " (01106845.0) etc., only pay attention to extracting medicinal components such as Radix Puerariae isoflavone, puerarin, pueraria starch, root of kudzu vine soluble polysaccharide then can not simultaneous extraction, cause the waste of root of kudzu vine raw material.
Summary of the invention purpose of the present invention just is to provide a kind of extracting method of root of kudzu vine effective ingredient, and it can the simultaneous extraction pueraria starch, root of kudzu vine soluble polysaccharide and Radix Puerariae isoflavone.
For achieving the above object, embodiment of the present invention are: the technology of a kind of root of kudzu vine simultaneous extraction pueraria starch, pueraria polysaccharide and Radix Puerariae isoflavone, it is characterized in that, and contain following processing step:
(1) water mill slurry, solid-liquid ratio 1: 6 will be added after the root of kudzu vine segment;
(2) adopt the compound cellulose enzymolysis, enzymatic hydrolysis condition: 45 ℃ of temperature, enzyme concn 0.06g/100g, 2 hours time, PH6, adjusting PH behind the enzymolysis is 8;
(3) will cross the slurries centrifugation of filtering fiber, wet starch, with the wet starch drying, pulverizing, sieving promptly get root of kudzu vine life starch;
(4) with the upper solution of centrifugation, at vacuum tightness-0.08Mpa, temperature is to be concentrated into soluble solid more than or equal to 15% under 60 ℃ the condition;
(5) add concentrated solution anhydrous or 95% ethanol, to alcohol concn be 75%, leave standstill centrifugation after 3 hours, obtain wet solubility pueraria polysaccharide, then drying, pulverize, sieve get final product pueraria polysaccharide;
(6) with the upper strata alcoholic solution after centrifugal, pump into the ethanol recovery tower, under the normal temperature condition of vacuum tightness one 0.08Mpa, reclaim ethanol, get Radix Puerariae isoflavone medicinal extract simultaneously, after drying treatment can get the Radix Puerariae isoflavone solid.
Gained Radix Puerariae isoflavone medicinal extract of the present invention can carry out microcapsule embedded, makes micro encapsulation Radix Puerariae isoflavone pressed powder.
The present invention utilizes zymotechnic, alcohol precipitation technology, vacuum decompression concentration technique etc., realized the simultaneous extraction of pueraria starch, pueraria polysaccharide and Radix Puerariae isoflavone, the extraction yield of pueraria starch and root of kudzu vine soluble polysaccharide extraction yield all reach more than 93%, the Radix Puerariae isoflavone extraction yield is more than 78%, not only changed the problem that waste is big, benefit is low that present domestic and international single extraction root of kudzu vine effective ingredient is caused, and having improved product recovery rate, benefit is with the obvious advantage.Simultaneously, the present invention adopts innoxious solvent ethanol, and has realized the alcoholic acid recycling, has reduced cost, guarantees extraction process and the finished product safety non-toxic.
Appended drawings is a process flow sheet of the present invention.The fresh root of kudzu vine B of A finished product of starch C pueraria polysaccharide D Radix Puerariae isoflavone medicinal extract E Radix Puerariae isoflavone product 1 soak, clean 2 cut off, arrangement 3 UV treatment 4 defibrinations 5 cross filtering fiber 6 centrifugations 7 starch sedimentations 8 washings 9 dry 10 supernatant liquors, 11 enzymolysis, 12 supernatant liquors, 13 vacuum concentration, 14 alcohol precipitations 15 and filter 16 ethanol for the second time and reclaim the dry 19 microcapsule Bao Li of 17 circulation ethanol, 18 precipitations.
Provide embodiments of the invention below the embodiment, its concrete processing step is as follows:
1, the cleaning of raw material: the fresh root of kudzu vine is fully soaked, remove sandy soil;
2, fragmentation: the root of kudzu vine is added slicing machine, be cut into every section 200-250 millimeter, again raw material after the cutting was added water by 1: 6 and add paste mill grinding, and use ultra-violet sterilization;
3, enzymolysis: adopt LS-1 complex cellulase enzymolysis under 45 ℃ of conditions, enzyme concn 0.06g/100g, enzymolysis time 2 hours, enzymolysis PH6, adding 2% sodium carbonate solution adjustment PH behind the enzymolysis immediately is 8;
4, cross the filtering fiber: adopt 120 orders to remove by filter fiber;
5, separating starch: the slurries after will filtering pump into whizzer, are 1500 rev/mins at rotating speed, centrifugation 10 minutes.The wet starch that obtains after centrifugation is being lower than under 60 ℃ the condition dryly, pulverizes, and crosses 120 mesh sieves and can produce the root of kudzu vine of market demand and give birth to starch;
6, vacuum concentration:, be to be concentrated into soluble solid more than or equal to 15% under 60 ℃ the condition in vacuum tightness for-0.08Mpa, temperature with the upper solution after centrifugal;
7, ethanol sedimentation polysaccharide: solution after will concentrating adds the ethanol of anhydrous or 95%, and alcohol concn is 75% to the solution, and leaving standstill after 3 hours at rotating speed is 1500 rev/mins, centrifugation 10 minutes.The wet solubility pueraria polysaccharide that obtains after centrifugation is being lower than under 60 ℃ the condition dryly, pulverizes, and crosses 120 mesh sieves and can produce pueraria polysaccharide;
8, ethanol reclaims and the concentrating of Radix Puerariae isoflavone: with the upper strata alcoholic solution after centrifugal, the vacuum tightness that pumps into the ethanol recovery tower is for the normal temperature condition of-0.08Mpa reclaims ethanol down, obtains soluble solid simultaneously and be 21.63% Radix Puerariae isoflavone medicinal extract;
9, Radix Puerariae isoflavone is microcapsule embedded: adopting cyclodextrine is the wall material, by per 100 milliliter of 60 gram, be dissolved in the spissated solution of Radix Puerariae isoflavone and heat, stir after homogeneous under the pressure of 40Mpa, pump into spray-drier again, in temperature in is 170 ℃, and temperature out is the dry micro encapsulation Radix Puerariae isoflavone pressed powder that gets final product of 103 ℃ condition.
Claims (2)
1, a kind of root of kudzu vine simultaneous extraction pueraria starch, pueraria polysaccharide and Radix Puerariae isoflavone technology is characterized in that, contain following processing step:
(1) water mill slurry, solid-liquid ratio 1: 6 will be added after the root of kudzu vine segment;
(2) adopt the compound cellulose enzymolysis, enzymatic hydrolysis condition: 45 ℃ of temperature, enzyme concn 0.06g/100g, 2 hours time, PH6, adjusting PH behind the enzymolysis is 8;
(3) will cross the slurries centrifugation of filtering fiber, wet starch, with the wet starch drying, pulverizing, sieving promptly get root of kudzu vine life starch;
(4) with the upper solution of centrifugation, at vacuum tightness-0.08Mpa, temperature is to be concentrated into soluble solid more than or equal to 15% under 60 ℃ the condition;
(5) add concentrated solution anhydrous or 95% ethanol, to alcohol concn be 75%, leave standstill centrifugation after 3 hours, obtain wet solubility pueraria polysaccharide, then drying, pulverize, sieve get final product pueraria polysaccharide;
(6) with the upper strata alcoholic solution after centrifugal, pump into the ethanol recovery tower, under the normal temperature condition of vacuum tightness-0.08Mpa, reclaim ethanol, get Radix Puerariae isoflavone medicinal extract simultaneously, after drying treatment can get the Radix Puerariae isoflavone solid.
2, root of kudzu vine simultaneous extraction pueraria starch according to claim 1, pueraria polysaccharide and Radix Puerariae isoflavone technology, it is characterized in that, gained Radix Puerariae isoflavone medicinal extract, adopting cyclodextrine is the wall material, by per 100 milliliter of 60 gram, is dissolved in the spissated solution of Radix Puerariae isoflavone and heats, stir after homogeneous under the pressure of 40Mpa, pumping into spray-drier again, is 170 ℃ in temperature in, and temperature out is the dry micro encapsulation Radix Puerariae isoflavone pressed powder that gets final product of 103 ℃ condition.
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| CNA031247547A CN1493695A (en) | 2003-08-22 | 2003-08-22 | Prcess of extracting kudzu vine root starch, kudiu vine root polysaccharide and kudzu vine root isoflavone from kudzu vine root synchronously |
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| CNA031247547A CN1493695A (en) | 2003-08-22 | 2003-08-22 | Prcess of extracting kudzu vine root starch, kudiu vine root polysaccharide and kudzu vine root isoflavone from kudzu vine root synchronously |
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Cited By (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1321993C (en) * | 2004-10-28 | 2007-06-20 | 浙江工业大学 | Method for extracting and separating isoflavone from kudzu slag |
| CN101570581A (en) * | 2009-06-10 | 2009-11-04 | 华中农业大学 | Method for comprehensively extracting pueraria polysaccharide and flavone |
| CN101190908B (en) * | 2006-11-22 | 2010-08-25 | 中国科学院过程工程研究所 | Method for extracting kudzu root fermentation fuel ethanol remainder flavone |
| CN101020720B (en) * | 2006-12-28 | 2010-12-15 | 宋桃森 | Process of extracting pueraria polysaccharide |
| CN101912148A (en) * | 2010-08-13 | 2010-12-15 | 川渝中烟工业公司 | Extraction method for blackberry lily extractum and application of blackberry lily extractum in cigarettes |
| CN101648999B (en) * | 2009-09-25 | 2011-05-11 | 汪盛明 | Method for separating pueraria starch milk impurities |
| CN102319284A (en) * | 2011-07-18 | 2012-01-18 | 句容茅宝葛业有限公司 | Method for extracting isoflavone from kudzuvine root efficiently |
| CN101704904B (en) * | 2009-11-13 | 2012-05-23 | 江苏大学 | Preparation method and application of kudzu polysaccharide extract |
| CN101736052B (en) * | 2009-12-25 | 2012-05-30 | 重庆正里元实业有限公司 | Technology for efficiently extracting radix puerariae starch and radix puerariae total isoflavone |
| CN102652556A (en) * | 2012-05-21 | 2012-09-05 | 褚玉云 | Preparation method of instant kudzuvine root particles |
| CN103478838A (en) * | 2013-09-25 | 2014-01-01 | 湖北省宏源药业有限公司 | Honeysuckle kudzuvine root beverage and preparation method thereof |
| CN105028496A (en) * | 2015-07-24 | 2015-11-11 | 安徽省圣丹生物化工有限公司 | Preparation method of efficient and environment-friendly bioherbicide |
| CN105028497A (en) * | 2015-07-24 | 2015-11-11 | 安徽省圣丹生物化工有限公司 | Preparation method of efficient herbicide micro-capsule |
| CN105053029A (en) * | 2015-07-24 | 2015-11-18 | 安徽省圣丹生物化工有限公司 | Preparation method of herbicide microcapsule containing kudzu vine active matters |
| CN105123778A (en) * | 2015-07-24 | 2015-12-09 | 安徽省圣丹生物化工有限公司 | Preparation method for high-performance durable bioherbicide |
| CN106279446A (en) * | 2016-08-29 | 2017-01-04 | 唐汉军 | Starch, fibrous polysaccharaide and the method for water solublity isoflavone is extracted from Radix Puerariae |
| CN107996913A (en) * | 2017-12-14 | 2018-05-08 | 牡丹江师范学院 | A kind of juice functional additive and preparation method and application |
| CN108030087A (en) * | 2018-01-29 | 2018-05-15 | 长沙爱扬医药科技有限公司 | A kind of pueraria lobata magma product |
| CN109078043A (en) * | 2018-09-27 | 2018-12-25 | 广东环凯微生物科技有限公司 | A kind of pueraria root health care product and preparation method thereof increasing bone density |
| CN109180829A (en) * | 2018-08-27 | 2019-01-11 | 杨鑫 | A kind of preparation method and application of Pueraria Flavonid and polyoses extract |
| CN111568943A (en) * | 2020-05-22 | 2020-08-25 | 安徽安力肽生物科技有限公司 | Preparation method of pueraria flavone microcapsules |
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2003
- 2003-08-22 CN CNA031247547A patent/CN1493695A/en active Pending
Cited By (27)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1321993C (en) * | 2004-10-28 | 2007-06-20 | 浙江工业大学 | Method for extracting and separating isoflavone from kudzu slag |
| CN101190908B (en) * | 2006-11-22 | 2010-08-25 | 中国科学院过程工程研究所 | Method for extracting kudzu root fermentation fuel ethanol remainder flavone |
| CN101020720B (en) * | 2006-12-28 | 2010-12-15 | 宋桃森 | Process of extracting pueraria polysaccharide |
| CN101570581A (en) * | 2009-06-10 | 2009-11-04 | 华中农业大学 | Method for comprehensively extracting pueraria polysaccharide and flavone |
| CN101648999B (en) * | 2009-09-25 | 2011-05-11 | 汪盛明 | Method for separating pueraria starch milk impurities |
| CN101704904B (en) * | 2009-11-13 | 2012-05-23 | 江苏大学 | Preparation method and application of kudzu polysaccharide extract |
| CN101736052B (en) * | 2009-12-25 | 2012-05-30 | 重庆正里元实业有限公司 | Technology for efficiently extracting radix puerariae starch and radix puerariae total isoflavone |
| CN101912148A (en) * | 2010-08-13 | 2010-12-15 | 川渝中烟工业公司 | Extraction method for blackberry lily extractum and application of blackberry lily extractum in cigarettes |
| CN101912148B (en) * | 2010-08-13 | 2013-05-22 | 川渝中烟工业有限责任公司 | Extraction method for blackberry lily extractum and application of blackberry lily extractum in cigarettes |
| CN102319284A (en) * | 2011-07-18 | 2012-01-18 | 句容茅宝葛业有限公司 | Method for extracting isoflavone from kudzuvine root efficiently |
| CN102652556A (en) * | 2012-05-21 | 2012-09-05 | 褚玉云 | Preparation method of instant kudzuvine root particles |
| CN103478838B (en) * | 2013-09-25 | 2014-12-03 | 湖北省宏源药业有限公司 | Honeysuckle kudzuvine root beverage and preparation method thereof |
| CN103478838A (en) * | 2013-09-25 | 2014-01-01 | 湖北省宏源药业有限公司 | Honeysuckle kudzuvine root beverage and preparation method thereof |
| CN105123778B (en) * | 2015-07-24 | 2018-02-16 | 安徽省圣丹生物化工有限公司 | A kind of preparation method of the lasting biological weed killer of high-performance |
| CN105028496B (en) * | 2015-07-24 | 2018-04-13 | 安徽省圣丹生物化工有限公司 | A kind of preparation method of high-efficiency environment friendly biological weed killer |
| CN105053029A (en) * | 2015-07-24 | 2015-11-18 | 安徽省圣丹生物化工有限公司 | Preparation method of herbicide microcapsule containing kudzu vine active matters |
| CN105123778A (en) * | 2015-07-24 | 2015-12-09 | 安徽省圣丹生物化工有限公司 | Preparation method for high-performance durable bioherbicide |
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| CN105028496A (en) * | 2015-07-24 | 2015-11-11 | 安徽省圣丹生物化工有限公司 | Preparation method of efficient and environment-friendly bioherbicide |
| CN105053029B (en) * | 2015-07-24 | 2018-04-03 | 安徽省圣丹生物化工有限公司 | A kind of preparation method of kudzu active material herbicide micro-capsule |
| CN105028497B (en) * | 2015-07-24 | 2018-04-06 | 安徽省圣丹生物化工有限公司 | A kind of preparation method of highy potent herbicide micro-capsule |
| CN106279446A (en) * | 2016-08-29 | 2017-01-04 | 唐汉军 | Starch, fibrous polysaccharaide and the method for water solublity isoflavone is extracted from Radix Puerariae |
| CN107996913A (en) * | 2017-12-14 | 2018-05-08 | 牡丹江师范学院 | A kind of juice functional additive and preparation method and application |
| CN108030087A (en) * | 2018-01-29 | 2018-05-15 | 长沙爱扬医药科技有限公司 | A kind of pueraria lobata magma product |
| CN109180829A (en) * | 2018-08-27 | 2019-01-11 | 杨鑫 | A kind of preparation method and application of Pueraria Flavonid and polyoses extract |
| CN109078043A (en) * | 2018-09-27 | 2018-12-25 | 广东环凯微生物科技有限公司 | A kind of pueraria root health care product and preparation method thereof increasing bone density |
| CN111568943A (en) * | 2020-05-22 | 2020-08-25 | 安徽安力肽生物科技有限公司 | Preparation method of pueraria flavone microcapsules |
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