CN101560262A - Process combination for extracting polysaccharide in hericium erinaceus mycelium cells and determination method thereof - Google Patents
Process combination for extracting polysaccharide in hericium erinaceus mycelium cells and determination method thereof Download PDFInfo
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- 150000004676 glycans Chemical class 0.000 title claims abstract description 62
- 229920001282 polysaccharide Polymers 0.000 title claims abstract description 62
- 239000005017 polysaccharide Substances 0.000 title claims abstract description 62
- 240000000588 Hericium erinaceus Species 0.000 title claims abstract description 31
- 235000007328 Hericium erinaceus Nutrition 0.000 title claims abstract description 31
- 238000000034 method Methods 0.000 title claims abstract description 29
- 230000008569 process Effects 0.000 title claims abstract description 19
- 239000007788 liquid Substances 0.000 claims abstract description 24
- 239000000843 powder Substances 0.000 claims abstract description 15
- 108090000790 Enzymes Proteins 0.000 claims abstract description 14
- 102000004190 Enzymes Human genes 0.000 claims abstract description 14
- 239000002994 raw material Substances 0.000 claims abstract description 8
- 238000000605 extraction Methods 0.000 claims description 38
- 238000002474 experimental method Methods 0.000 claims description 17
- 239000000284 extract Substances 0.000 claims description 17
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 14
- 241000123222 Hericium Species 0.000 claims description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 11
- 230000007062 hydrolysis Effects 0.000 claims description 9
- 238000006460 hydrolysis reaction Methods 0.000 claims description 9
- 238000005303 weighing Methods 0.000 claims description 9
- 239000006228 supernatant Substances 0.000 claims description 7
- 238000001291 vacuum drying Methods 0.000 claims description 7
- 238000001704 evaporation Methods 0.000 claims description 6
- 230000008020 evaporation Effects 0.000 claims description 6
- 238000000855 fermentation Methods 0.000 claims description 6
- 230000004151 fermentation Effects 0.000 claims description 6
- 238000004062 sedimentation Methods 0.000 claims description 6
- 102000003712 Complement factor B Human genes 0.000 claims description 5
- 108090000056 Complement factor B Proteins 0.000 claims description 5
- 244000309464 bull Species 0.000 claims description 5
- 230000009471 action Effects 0.000 claims description 2
- 230000007071 enzymatic hydrolysis Effects 0.000 claims description 2
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 claims description 2
- 210000004027 cell Anatomy 0.000 description 16
- 229940088598 enzyme Drugs 0.000 description 9
- 238000005516 engineering process Methods 0.000 description 8
- 238000004519 manufacturing process Methods 0.000 description 8
- 230000000694 effects Effects 0.000 description 6
- 230000003834 intracellular effect Effects 0.000 description 6
- 239000000243 solution Substances 0.000 description 5
- 239000003814 drug Substances 0.000 description 4
- 238000002386 leaching Methods 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- 241000233866 Fungi Species 0.000 description 3
- 102000011759 adducin Human genes 0.000 description 3
- 108010076723 adducin Proteins 0.000 description 3
- 239000000470 constituent Substances 0.000 description 3
- 239000000706 filtrate Substances 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 238000000926 separation method Methods 0.000 description 3
- 239000002904 solvent Substances 0.000 description 3
- 108010059892 Cellulase Proteins 0.000 description 2
- 102000035195 Peptidases Human genes 0.000 description 2
- 108091005804 Peptidases Proteins 0.000 description 2
- 108010059820 Polygalacturonase Proteins 0.000 description 2
- 210000002421 cell wall Anatomy 0.000 description 2
- 229940106157 cellulase Drugs 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 238000010438 heat treatment Methods 0.000 description 2
- 239000004615 ingredient Substances 0.000 description 2
- 239000001814 pectin Substances 0.000 description 2
- 229920001277 pectin Polymers 0.000 description 2
- 235000010987 pectin Nutrition 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 238000003809 water extraction Methods 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 208000007882 Gastritis Diseases 0.000 description 1
- 208000007107 Stomach Ulcer Diseases 0.000 description 1
- 230000001133 acceleration Effects 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 230000000975 bioactive effect Effects 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 229960000074 biopharmaceutical Drugs 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
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- 238000001311 chemical methods and process Methods 0.000 description 1
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- Coloring Foods And Improving Nutritive Qualities (AREA)
- Medicines Containing Plant Substances (AREA)
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Abstract
The invention relates to a process combination for extracting polysaccharide in hericium erinaceus mycelium cells. The solid-liquid ratio according to weight is 1: 10-1: 60, the adding amount of complex enzyme accounts for 0.5 percent to 5.0 percent of the weight of the raw materials, the range of ultrasonic power is 100w to 500w, the range of enzymolysis time is 40min to 100min, and the range of enzymolysis temperature is 30 DEG C to 60 DEG C, and the range of enzymolysis pH value is 4.0 to 8.0. The determination method of the process combination for extracting polysaccharide in hericium erinaceus mycelium cells is as follows: high-quality mycelium is selected to be deeply fermented, thus obtaining mycelium dry powder; the solid-liquid ratio is determined; the adding amount of complex enzyme is determined; and the optimal extracting process combination for extracting mycelium dry powder polysaccharide in an ultrasonic-complex enzyme method is determined.
Description
Technical field
The present invention relates to the bio-pharmaceuticals production technology, specifically a kind of process combination and definite method thereof of extracting polysaccharide in hericium erinaceus mycelium cells.
Background technology
Hericium erinaceus (Bull. Ex Fr.) Pers. is famous dietotherapeutic bacterium, is used as medicine with sporophore, and the flat flavor of property is sweet, has functions aid digestion, sharp the five internal organs, is the good medicine of multiple digestive tract diseases such as treatment chronic gastritis, duodenal ulcer, stomach swelling, stomach ulcer.It is " high protein, lower fat " food, is a kind of good health care fungi.
Hericium erinaceum polysaccharide is widely used in fields such as medicine, food, market demand grows with each passing day, but the production of hericium erinaceum polysaccharide nearly all is to extract from sporophore at present, the Hericium erinaceus (Bull. Ex Fr.) Pers. wild resource lacks, the tame cycle is long, floor space is big, is subject to seasonal restrictions again, thereby has influenced making full use of of Hericium erinaceus (Bull. Ex Fr.) Pers..Utilize the submerged fermentation technology to produce the hericium mycelium polysaccharide, its biological effect and hericium erinaceus fruiting body polysaccharide much at one, and it is with short production cycle, cost is low, output is big, has industrialized prospect of production.
The hericium mycelium polysaccharide that forms in submerged fermentation divides exocellular polysaccharide and intracellular polyse two portions, and intracellular polyse has accounted for 60-90%, is the main source of hericium erinaceum polysaccharide mycelium polysaccharides.Extraction polysaccharide mode commonly used has hot water extraction, acid leaching extraction method, alkali extraction method, enzyme extraction method etc.Because the existence of mycelial cell wall, intracellular component is difficult to be discharged into smoothly outside the born of the same parents, non-polysaccharide materials such as pectin substance that cell is inside and outside and protein also make extracting efficiency of polysaccharides and purity be affected, traditional some extract that polysaccharide modes cause leaching process length consuming time, the extraction yield of hericium erinaceum polysaccharide low, polysaccharide content is relatively low in the extract, have perhaps destroyed the molecular activity structure picture of polysaccharide because of chemical reagents reaction.Therefore, seeking efficiently, extraction method of polysaccharides is that development hericium mycelium polysaccharide is the key in this field.
Natural medicinal ingredients mostly is product in the cell greatly, how need carry out cytoclasis during extraction, and existing mechanical and chemical process is difficult to obtain the ideal crushing effect sometimes.Ultrasonic technology is applied to the extraction of hericium mycelium polysaccharide, be effects such as the judder that utilizes ultrasonic wave to produce, high acceleration, intensive cavitation effect, stirring, quicken effective constituent and enter solvent, thereby raising leaching yield, shorten extraction time, can avoid simultaneously proposing the influence of composition.This technology is widely used in Chinese medicine extracts active ingredients, separation and preparation technology.
In the leaching process of mycelium polysaccharides, also can utilize suitable enzyme, under relatively mild condition, cell tissue is decomposed, quicken the release of effective constituent, thereby improve polysaccharide yield.The prozyme of cellulase, polygalacturonase and proteolytic enzyme composition is used for the extraction of polysaccharide, to remove the compositions such as pectin, Mierocrystalline cellulose and protein on cell walls born of the same parents and the cell membrane, make mycelial cell break smoothly, help promoting the stripping of intracellular polyse, improve extraction yield, shorten extraction time, reduce energy consumption and effectively preserve the bioactive purpose of polysaccharide to reach.But can improve production cost owing to add enzyme extraction, bring difficulty also for simultaneously the separation of product, on addition, want strict control.
Summary of the invention
The objective of the invention is to, utilize ultrasonic wave hericium mycelium body tissue to be handled as supplementary means, the prozyme of forming with cellulase, polygalacturonase and proteolytic enzyme obtains hericium erinaceum polysaccharide to hericium mycelium infusion enzymolysis simultaneously, a kind of extraction effect better than ordinary method that have is provided, extracts polysaccharide in hericium erinaceus mycelium cells technology and definite method thereof with lower cost.
Technical scheme of the present invention is: a kind of process combination of extracting polysaccharide in hericium erinaceus mycelium cells, solid-liquid ratio is 1 by mass ratio: 10-1: 60, the prozyme add-on is between the 0.5%-5.0% of raw materials quality, the ultrasonic power value range is between the 100W-500W, the enzymolysis time value range is between the 40min-100min, the hydrolysis temperature value range is between 30 ℃-60 ℃, and enzymolysis pH value value range is between the 4.0-8.0.
Described a kind of definite method of extracting the process combination of polysaccharide in hericium erinaceus mycelium cells is:
1) the mycelium dry powder of selected fine hericium mycelium submerged fermentation gained
2) determine solid-liquid ratio
The mycelium dry powder that step 1) is selected fully dissolves in the water, and the reasonable solid-liquid ratio of hericium mycelium was at 1: 10 (g: g)-1: 60 (g: g).Under this solid-liquid ratio condition, carry out hot water lixiviate polysaccharide experiment, the control extraction temperature is in 50～95 ℃, lixiviate pH value 4.0～7.5, extraction time 40min～120min scope.After 5～15 times of the vat liquor evaporation concentration, add 95% ethanol sedimentation of 4 times of volumes, 2000rmp is centrifugal, and supernatant liquor is removed in the back, and vacuum-drying is to constant weight, weighing.By relatively, determine the highest solid-liquid ratio of polysaccharide in hericium erinaceus mycelium cells extraction yield that the hot water lixiviate obtains, be exactly the best solid-liquid ratio of this kind.
3) determine the addition of prozyme
According to top step 2) in the vat liquor raw material that is made into of best solid-liquid ratio in, add rational prozyme amount, scope is between the 0.5%-5.0% of vat liquor raw materials quality.Under suitable enzymatic hydrolysis condition, carry out experiment of single factor, determine the addition of the suitableeest prozyme.Under the addition condition of this prozyme, the control extraction temperature is in 40～65 ℃, lixiviate pH value 4.0～6.5, extraction time 40min～80min scope.After 5～15 times of the vat liquor evaporation concentration, add 95% ethanol sedimentation of 4 times of volumes, 2000rmp is centrifugal, and supernatant liquor is removed in the back, and vacuum-drying is to constant weight, weighing.By relatively, determine the addition of the prozyme that the polysaccharide in hericium erinaceus mycelium cells extraction yield is the highest, both be the addition of the best complex enzyme of this kind.
4) determine that ultrasonic wave-prozyme solution extracts the optimum extraction process combination of this mycelium dry powder polysaccharide
The complex enzyme hydrolysis that carries out Hericium erinaceus (Bull. Ex Fr.) Pers. fermentation mycelium polysaccharide under the ultrasonic wave booster action extracts.In step 2) and 3) solid-liquid ratio and the condition of prozyme addition under, with ultrasonic power (factor A), enzymolysis time (factor B), hydrolysis temperature (factor C), enzymolysis pH value (factor C) is analytic target, carry out the experiment of 4 factors, 3 horizontal quadratures, obtain the optimum extraction process combination.According to the previous experiments result, the scope of experiment value of factor A is between the 100W-500W, and the scope of experiment value of factor B is between the 40min-100min, and the scope of experiment value of factor C is that the scope of experiment value of factor D is between the 4.0-8.0 between 30 ℃-60 ℃.Extract under combination process condition at this, carry out mycelium polysaccharides and extract, after 5～15 times of the vat liquor evaporation concentration, add 95% ethanol sedimentation of 4 times of volumes, 2000rmp is centrifugal, and supernatant liquor is removed in the back, and vacuum-drying is to constant weight, weighing.By relatively, determine the highest process combination of polysaccharide in hericium erinaceus mycelium cells extraction yield, both be the optimum extraction process combination of this mycelium dry powder.
The present invention compared with prior art has the following advantages:
1, judder, the speed of superelevation, the intensive cavitation effect that utilizes ultrasonic wave to produce can quicken effective ingredient and enter solvent.
2, these non-polysaccharide fractions have been decomposed in the adding of prozyme effectively, under the synergy of ultrasonic wave mechanism, can improve the yield of polysaccharide more up hill and dale, shorten extraction time, save solvent, help protecting polysaccharide effective constituent.
3, technology can significantly reduce production costs in the extraction process of hericium mycelium polysaccharide is transformed, and improves separation efficiency, and is adapted at applying in the suitability for industrialized production.
Embodiment
The hericium mycelium dry powder polysaccharide extracting process combination that Qingyuan County, Zhejiang Province grid medicinal fungus company limited produces, its composition is: solid-liquid ratio is 1: 30 by mass ratio, the prozyme add-on is 2%, ultrasonic power is 200W, enzymolysis time 50min, 55 ℃ of hydrolysis temperatures, enzymolysis pH value 5.0.
Described a kind of definite method of extracting the polysaccharide in hericium erinaceus mycelium cells process combination is:
1) the hericium mycelium dry powder of selected Zhejiang Province Qingyuan County grid medicinal fungus company production is as extracting raw material.
2) determine solid-liquid ratio
Extract polysaccharide by the hot water extraction.Choosing the mycelium dry powder 100g of step 1), is analytical factor to add the water multiple, and 10,20,30,40,50,60 times (quality multiples) are set, and is heated to 90 ℃ and keeps 3 hours, filters.Filtrate is concentrated into 100ml at rotatory evaporator, is cooled to normal temperature.95% ethanol that adds 400ml stirred 10 minutes with glass stick in concentrated solution simultaneously, with centrifugal 2 minutes of mixed solution 1000rmp, removed supernatant liquor, the mycelium polysaccharides precipitation, vacuum-drying weighing to the constant weight.After finding to add the water of 30 times of weight (3000g), the multiple extraction acquisition polysaccharide quantitative changeization that increases water again is little, so determine that solid-liquid ratio is 1: 30.
3) determine the addition of prozyme
Extract polysaccharide by the prozyme solution.With the compound enzymic preparation addition is empirical factor, at solid-liquid ratio is 1: 30,45 ℃ of temperature, pH6.5, under the condition of enzymolysis time 80min, the prozyme addition is set to 0.5%, 1.0%, 1.5%, 2.0%, 2.5%, 3.0%, 3.5% (mass percent of mycelium dry powder relatively), behind the enzymolysis time 80min, and 95 ℃ of heating enzyme 5min that goes out, 80 ℃ of hot water continue lixiviate 60min, filter the rear filtrate treatment step with 1, the polysaccharide in hericium erinaceus mycelium cells of acquisition, weighing.Find that the prozyme addition is set to after 2.0%, increase the prozyme addition again, the hericium erinaceus mycelium intracellular polyse that the enzymolysis lixiviate obtains changes little, so determine that the prozyme addition is 2.0%.
4) optimum extraction process of determining this mycelium dry powder makes up
Extract polysaccharide by ultrasonic wave-prozyme solution.At solid-liquid ratio is 1: 30, under the condition of enzyme concentration 2.0%, with ultrasonic power (factor A), enzymolysis time (factor B), hydrolysis temperature (factor C), enzymolysis pH value (factor C) is analytic target, carries out the experiment of 4 factors, 3 horizontal quadratures, and quadrature level of factor table is as follows:
After the operation of the ultrasonic wave under each Orthogonal Composite condition-prozyme solution, 95 ℃ of heating of enzymolysis time enzyme 5min that goes out, 80 ℃ of hot water continue lixiviate 60min, filter the rear filtrate treatment step with 1, the hericium erinaceus mycelium intracellular polyse of acquisition, weighing.
Test-results shows, at A
2B
1C
3D
1The process combination condition under, polysaccharide extract rate is the highest in the mycelium cell, promptly when solid-liquid ratio be 1: 30, under enzyme concentration 2.0% condition, ultrasonic power is 200W, enzymolysis time 50min, 55 ℃ of hydrolysis temperatures, during enzymolysis pH value 5.0, polysaccharide extract rate is the highest in the mycelium cell.
Claims (2)
1, a kind of process combination of extracting polysaccharide in hericium erinaceus mycelium cells, it is characterized in that each component is: solid-liquid ratio is 1 by mass ratio: 10-1: 60, the prozyme add-on is between the 0.5%-5.0% of raw materials quality, the ultrasonic power value range is between the 100W-500W, the enzymolysis time value range is between the 40min-100min, the hydrolysis temperature value range is between 30 ℃-60 ℃, and enzymolysis pH value value range is between the 4.0-8.0.
2, according to the described a kind of definite method of extracting the process combination of polysaccharide in hericium erinaceus mycelium cells of claim 1, it is characterized in that:
1) the mycelium dry powder of selected fine mycelium submerged fermentation gained
2) determine solid-liquid ratio
The mycelium dry powder that step 1) is selected fully dissolves in the water, the reasonable solid-liquid ratio of hericium mycelium was at 1: 10 (g: g)-1: 60 (g: g), under this solid-liquid ratio condition, carry out the experiment of hot water lixiviate polysaccharide, the control extraction temperature is 50～95 ℃, lixiviate pH value 4.0～7.5, in extraction time 40min～120min scope, after 5～15 times of the vat liquor evaporation concentration, 95% ethanol sedimentation that adds 4 times of volumes, 2000rmp is centrifugal, and supernatant liquor is removed in the back, vacuum-drying is to constant weight, and weighing is by comparing, determining the highest solid-liquid ratio of polysaccharide in hericium erinaceus mycelium cells extraction yield that the hot water lixiviate obtains, is exactly the best solid-liquid ratio of this kind;
3) determine the addition of prozyme
According to top step 2) in the vat liquor raw material that is made into of best solid-liquid ratio in, add rational prozyme amount, scope is between the 0.5%-5.0% of vat liquor raw materials quality, under suitable enzymatic hydrolysis condition, carry out experiment of single factor, determine the addition of the suitableeest prozyme, under the addition condition of this prozyme, the control extraction temperature is in 40～65 ℃, lixiviate pH value 4.0～6.5, extraction time 40min～80min scope.After 5～15 times of the vat liquor evaporation concentration, 95% ethanol sedimentation that adds 4 times of volumes, 2000rmp is centrifugal, and supernatant liquor is removed in the back, vacuum-drying is to constant weight, weighing, by relatively, determine the addition of the prozyme that the polysaccharide in hericium erinaceus mycelium cells extraction yield is the highest, both be the addition of the best complex enzyme of this kind;
4) determine that ultrasonic wave-prozyme solution extracts the optimum extraction process of this mycelium dry powder polysaccharide and be combined in the complex enzyme hydrolysis that carries out Hericium erinaceus (Bull. Ex Fr.) Pers. fermentation mycelium polysaccharide under the ultrasonic wave booster action and extract, in step 2) and 3) solid-liquid ratio and the condition of prozyme addition under, with ultrasonic power (factor A), enzymolysis time (factor B), hydrolysis temperature (factor C), enzymolysis pH value (factor C) is an analytic target, carry out the experiment of 4 factors, 3 horizontal quadratures, the combination of acquisition optimum extraction process, according to the previous experiments result, the scope of experiment value of factor A is between the 100W-500W, the scope of experiment value of factor B is between the 40min-100min, the scope of experiment value of factor C is between 30 ℃-60 ℃, the scope of experiment value of factor D is between the 4.0-8.0, extract under the combination process condition at this, carrying out mycelium polysaccharides extracts, after 5～15 times of the vat liquor evaporation concentration, 95% ethanol sedimentation that adds 4 times of volumes, 2000rmp is centrifugal, and supernatant liquor is removed in the back, vacuum-drying is to constant weight, weighing, by comparing, determining the highest process combination of polysaccharide in hericium erinaceus mycelium cells extraction yield, both has been the optimum extraction process combination of this mycelium dry powder.
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102382199A (en) * | 2011-09-02 | 2012-03-21 | 广东太阳神集团有限公司 | High yield energy saving preparation method of Hericium erinaceus polysaccharide |
| CN104072632A (en) * | 2014-07-15 | 2014-10-01 | 江苏阜丰生物科技有限公司 | Method for efficiently extracting maitake mycelia polysaccharides through submerged fermentation production |
| CN104231110A (en) * | 2014-10-21 | 2014-12-24 | 哈尔滨艾博雅食品科技开发有限公司 | Extraction method for crude polysaccharides of hericium erinaceus |
| CN105919057A (en) * | 2016-04-20 | 2016-09-07 | 中国农业科学院特产研究所 | Bearded tooth mushroom gel and preparation method thereof |
| CN108497118A (en) * | 2018-04-11 | 2018-09-07 | 广州汝丽多食品科技有限公司 | A kind of sobering-up health tea and preparation method thereof |
| CN110623251A (en) * | 2019-05-17 | 2019-12-31 | 成都市农林科学院 | Preparation method of medicine and food dual-purpose hericium erinaceus stomach-nourishing nutrient solution |
| CN110894518A (en) * | 2019-11-05 | 2020-03-20 | 上海加新生物科技有限公司 | Preparation method of hericium erinaceus small peptide |
| CN112522344A (en) * | 2020-12-23 | 2021-03-19 | 上海市农业科学院 | Method for solid-state enzymolysis of hericium erinaceus sporophore powder |
-
2009
- 2009-06-01 CN CNA2009100992148A patent/CN101560262A/en not_active Withdrawn
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102382199A (en) * | 2011-09-02 | 2012-03-21 | 广东太阳神集团有限公司 | High yield energy saving preparation method of Hericium erinaceus polysaccharide |
| CN102382199B (en) * | 2011-09-02 | 2012-12-12 | 广东太阳神集团有限公司 | High yield energy saving preparation method of Hericium erinaceus polysaccharide |
| CN104072632A (en) * | 2014-07-15 | 2014-10-01 | 江苏阜丰生物科技有限公司 | Method for efficiently extracting maitake mycelia polysaccharides through submerged fermentation production |
| CN104231110A (en) * | 2014-10-21 | 2014-12-24 | 哈尔滨艾博雅食品科技开发有限公司 | Extraction method for crude polysaccharides of hericium erinaceus |
| CN105919057A (en) * | 2016-04-20 | 2016-09-07 | 中国农业科学院特产研究所 | Bearded tooth mushroom gel and preparation method thereof |
| CN108497118A (en) * | 2018-04-11 | 2018-09-07 | 广州汝丽多食品科技有限公司 | A kind of sobering-up health tea and preparation method thereof |
| CN110623251A (en) * | 2019-05-17 | 2019-12-31 | 成都市农林科学院 | Preparation method of medicine and food dual-purpose hericium erinaceus stomach-nourishing nutrient solution |
| CN110894518A (en) * | 2019-11-05 | 2020-03-20 | 上海加新生物科技有限公司 | Preparation method of hericium erinaceus small peptide |
| CN112522344A (en) * | 2020-12-23 | 2021-03-19 | 上海市农业科学院 | Method for solid-state enzymolysis of hericium erinaceus sporophore powder |
| CN112522344B (en) * | 2020-12-23 | 2022-09-13 | 上海市农业科学院 | Method for solid-state enzymolysis of hericium erinaceus sporophore powder |
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