CN105994798A - Method for preparing soluble tea pills from wild pueraria flavonoid extract - Google Patents
Method for preparing soluble tea pills from wild pueraria flavonoid extract Download PDFInfo
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- CN105994798A CN105994798A CN201610331301.1A CN201610331301A CN105994798A CN 105994798 A CN105994798 A CN 105994798A CN 201610331301 A CN201610331301 A CN 201610331301A CN 105994798 A CN105994798 A CN 105994798A
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- tea ball
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23F—COFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
- A23F3/00—Tea; Tea substitutes; Preparations thereof
- A23F3/34—Tea substitutes, e.g. matè; Extracts or infusions thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
- A61K36/488—Pueraria (kudzu)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/46—Ingredients of undetermined constitution or reaction products thereof, e.g. skin, bone, milk, cotton fibre, eggshell, oxgall or plant extracts
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/0087—Galenical forms not covered by A61K9/02 - A61K9/7023
- A61K9/0095—Drinks; Beverages; Syrups; Compositions for reconstitution thereof, e.g. powders or tablets to be dispersed in a glass of water; Veterinary drenches
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/19—Preparation or pretreatment of starting material involving fermentation using yeast, bacteria or both; enzymatic treatment
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Chemical & Material Sciences (AREA)
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- Medicines Containing Plant Substances (AREA)
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Abstract
The invention discloses a method for preparing soluble tea pills from a wild pueraria flavonoid extract. The method comprises steps as follows: wild pueraria is subjected to enzymatic extraction at the low temperature, and the wild pueraria flavonoid extract is prepared; the wild pueraria flavonoid extract is mixed with liquorice roots, honey, menthol and beta-cyclodextrin are added, and the soluble tea pills are prepared through sieving. According to the method for preparing the soluble tea pills from the wild pueraria flavonoid extract, the adopted wild pueraria flavonoid extract is obtained through enzymatic extraction at the low temperature, is high in purity and has the relatively high activity; meanwhile, the prepared soluble tea pills have multiple good effects of reducing vascular resistance, improving cardio-cerebral blood circulation, reducing myocardial oxygen consumption and the like due to the fact that the tea pills contain the high-purity pueraria flavonoid extract, the preparation technology is simple and convenient, and all components in the tea pills can be effectively reserved.
Description
Technical field
The present invention relates to the preparation method of a kind of solubility tea ball, be specifically related to one and utilize Wild Pueraria flavone to carry
Take the method that thing prepares solubility tea ball.
Background technology
At present, drink tea series products a multitude of names, effect is complicated, and its preparation technology is different；Tea writes as one
The health promoting beverage of name, in China's history of existing nearly more than 4,000 years, it is with cocoa, coffee and claims the world three big nothing
Alcoholic beverage, and tea is first of the big beverage in the world three；Tea itself have heat clearing away, detoxify, strengthen the spleen and stomach etc. many
Plant effect, according to separation and the qualification of modern science, Folium Camelliae sinensis reach kind more than 450 containing organic chemical composition,
Inorganic mineral element reaches kind more than 40, basis tea polyphenols be commonly called as tea tannin have antioxidation, anti-halmatogenesis,
Antitumor, reduction Blood Cholesterol and low-density ester gp content, suppression blood pressure rise, suppression platelet is coagulated
The effects such as collection, product allergy antibacterial, anti-；The traditional Chinese medical science thinks that tea not only has the therapeutic efficacy to many sections disease, and
There are good life lengthening, the anti-ageing effect kept fit.At present, worldwide, making a lot of variety of tea, have
With the brewed herbal tea of plant flowers, leaf, seed, root, such as chrysanthemum scented tea etc..But the Folium Camelliae sinensis majority on market is only
There is the shortcoming that effect is single, therefore seek that there is reduction vascular resistance, improve heart and brain blood circulation, reduce the heart
The multiple pharmacological effect solubility tea balls such as myocardial oxygen consumption, have good economic outlook and practical value.
Radix Puerariae is the dry root of legume pueraria lobata, and its sweet in the mouth, pungent, property are put down, and be Chinese crude drug and nutrition is rich
Rich edible raw material, main chemical compositions is the flavone compounds such as puerarin, Dai, Semen sojae atricolor former times.Modern medicine
Reason research shows, Radix Puerariae flavone has reduction vascular resistance, improves heart and brain blood circulation, reduces myocardial oxygen consumption etc.
Multiple pharmacological effect.
Summary of the invention
Goal of the invention: the problem existed for prior art, the present invention provides one to utilize Wild Pueraria flavone to carry
Take the method that thing prepares solubility tea ball.
Technical scheme: a kind of side utilizing Wild Pueraria flavone extract to prepare solubility tea ball
Method, it is characterised in that comprise the steps:
(1) Wild Pueraria flavone extract and Radix Glycyrrhizae are mixed, boiling, filter, merging filtrate；
(2) by filtrate concentration, lyophilization, prepare mixture, in mixture, add Mel, Mentholum
And cycloheptaamylose, mix homogeneously；
(3) being sieved by homomixture, the granule after sieving dispenses makes spherical tea ball in pill container；
(4) gained tea ball is sieved successively, be dried, after being toasted by dried tea ball, take out nature
It is cooled to room temperature；
(5) the tea ball after cooling is screened out tiny foreign particle, obtain solubility tea ball, test package afterwards.
Further, the preparation method of described step (1) Wild Pueraria flavone extract, comprise the steps:
By Wild Pueraria lyophilization, pulverize and sieve；Wild Pueraria powder after pulverizing adds the phosphoric acid hydrogen of pre-cooling in advance
Disodium-citrate buffer solution, adds compound enzyme and ferrous carbonate, at 15-20 DEG C of enzymolysis 40-60min, pH value
5.5-6.5；After enzymolysis, enzymolysis solution is filtered, constant volume, obtain Radix Puerariae extracting solution, by extracting solution by ultra micro amplitude
Penetrating process, then concentrating under reduced pressure, lyophilization prepare Kudzu Flavonoids Extracts.
Further, boiling 2 times-4 times in described step (1), each time is 1-3h.
Further, homomixture is crossed 40-60 mesh sieve by described step (3).
Further, gained tea ball is crossed and is sieved successively as crossing No. 3.5 compass screen surfaces and No. 4 sieves by described step (4)
The end or No. 3 compass screen surfaces and No. 3.5 sieve ends, sieve.
Further, described step (4) is dried to moisture below 5%.
The present invention also provides for a kind of solubility tea ball, including made by the raw material of following parts by weight: Radix Puerariae flavone
Extract 50-70 part, Radix Glycyrrhizae 20-40 part, Mel 5-15 part, Mentholum 7-12 part, cycloheptaamylose 1-3
Part.
Beneficial effect: the present invention utilizes the method that Wild Pueraria flavone extract prepares solubility tea ball, employing
Kudzu Flavonoids Extracts is obtained by enzyme process extract at low temperature, and Kudzu Flavonoids Extracts purity is high, and has higher
Activity；Solubility tea ball obtained by Tong Shi is owing to containing highly purified Kudzu Flavonoids Extracts, having good
Reduction vascular resistance, improve heart and brain blood circulation, reduce the multiple efficacies such as myocardial oxygen consumption, and prepare work
Skill is simple, convenient, each component can being effectively kept in tea ball.
Detailed description of the invention
Below in conjunction with embodiment, the present invention will be further described.
Embodiment 1
A kind of solubility tea ball of the present embodiment, including made by the raw material of following parts by weight: Radix Puerariae flavone carries
Take thing 50 parts, 20 parts of Radix Glycyrrhizae, 5 parts of Mel, Mentholum 7 parts, cycloheptaamylose 1 part.
A kind of method utilizing Wild Pueraria flavone extract to prepare solubility tea ball of the present embodiment, including walking as follows
Rapid:
Prepared by Kudzu Flavonoids Extracts: take Wild Pueraria 1000g and carry out lyophilization, carry out powder by pulverizer
Broken mistake 40 mesh sieve；Wild Pueraria powder after pulverizing adds pH is 64 DEG C of phosphoric acid hydrogen of 10L pre-cooling in advance
Disodium-citrate buffer solution, adds 2g cellulase and the compound enzyme of 1g pectase and 0.15g ferrous carbonate,
At 15 DEG C of enzymolysis 60min, pH value 5.5；After enzymolysis, enzymolysis solution is filtered, constant volume, obtains Radix Puerariae extracting solution,
By extracting solution by ultramicrowave radiation treatment, ultramicrowave radiation treatment power 50W, temperature controls to carry at 30 DEG C
Taking 30min, then concentrating under reduced pressure, lyophilization prepare Kudzu Flavonoids Extracts.
The preparation of solubility tea ball, comprises the steps:
(1) above-mentioned prepared Wild Pueraria flavone extract and Radix Glycyrrhizae are mixed, boiling 2 times, every time
3h, filters, merging filtrate；
(2) by filtrate concentration, lyophilization, prepare mixture, in mixture, add Mel, Mentholum
And cycloheptaamylose, mix homogeneously；
(3) homomixture being crossed 40 mesh sieves, the granule after sieving dispenses makes spherical tea ball in pill container；
(4) gained tea ball is crossed successively No. 3.5 compass screen surfaces and No. 4 sieve ends, be dried to moisture 5%, will be dried
After tea ball toast after, take out naturally cool to room temperature；
(5) the tea ball after cooling is screened out tiny foreign particle, obtain solubility tea ball, test package afterwards.
Embodiment 2
A kind of solubility tea ball of the present embodiment, including made by the raw material of following parts by weight: Radix Puerariae flavone carries
Take thing 70 parts, 40 parts of Radix Glycyrrhizae, 15 parts of Mel, Mentholum 12 parts, cycloheptaamylose 3 parts.
A kind of method utilizing Wild Pueraria flavone extract to prepare solubility tea ball of the present embodiment, including walking as follows
Rapid:
Prepared by Kudzu Flavonoids Extracts: take Wild Pueraria 1000g and carry out lyophilization, carry out powder by pulverizer
Broken mistake 60 mesh sieve；Wild Pueraria powder after pulverizing adds pH is 6 10 DEG C of phosphoric acid of 10L pre-cooling in advance
Disodium hydrogen-citrate buffer solution, adds 5g cellulase and the compound enzyme of 1g pectase and 0.6g carbonic acid is sub-
Ferrum, at 20 DEG C of enzymolysis 40min, pH value 6.5；After enzymolysis, enzymolysis solution is filtered, constant volume, obtain Radix Puerariae and extract
Liquid, by extracting solution by ultramicrowave radiation treatment, ultramicrowave radiation treatment power 70W, temperature controls at 40 DEG C
Extracting 20min, then concentrating under reduced pressure, lyophilization prepare Kudzu Flavonoids Extracts.
The preparation of solubility tea ball, comprises the steps:
(1) above-mentioned prepared Wild Pueraria flavone extract and Radix Glycyrrhizae are mixed, boiling 4 times, every time
1h, filters, merging filtrate；
(2) by filtrate concentration, lyophilization, prepare mixture, in mixture, add Mel, Mentholum
And cycloheptaamylose, mix homogeneously；
(3) homomixture being crossed 60 mesh sieves, the granule after sieving dispenses makes spherical tea ball in pill container；
(4) gained tea ball is crossed successively No. 3 compass screen surfaces and No. 3.5 sieve ends, be dried to moisture 3%, will be dried
After tea ball toast after, take out naturally cool to room temperature；
(5) the tea ball after cooling is screened out tiny foreign particle, obtain solubility tea ball, test package afterwards.
Embodiment 3
A kind of solubility tea ball of the present embodiment, including made by the raw material of following parts by weight: Radix Puerariae flavone carries
Take thing 60 parts, 30 parts of Radix Glycyrrhizae, 10 parts of Mel, Mentholum 9 parts, cycloheptaamylose 2 parts.
A kind of method utilizing Wild Pueraria flavone extract to prepare solubility tea ball of the present embodiment, including walking as follows
Rapid:
Prepared by Kudzu Flavonoids Extracts: take Wild Pueraria 1000g and carry out lyophilization, carry out powder by pulverizer
Broken mistake 50 mesh sieve；Wild Pueraria powder after pulverizing adds pH is 67 DEG C of phosphoric acid hydrogen of 10L pre-cooling in advance
Disodium-citrate buffer solution, adds 4g cellulase and the compound enzyme of 1g pectase and 0.4g ferrous carbonate,
At 18 DEG C of enzymolysis 50min, pH value 6.0；After enzymolysis, enzymolysis solution is filtered, constant volume, obtains Radix Puerariae extracting solution,
By extracting solution by ultramicrowave radiation treatment, ultramicrowave radiation treatment power 60W, temperature controls to carry at 35 DEG C
Taking 25min, then concentrating under reduced pressure, lyophilization prepare Kudzu Flavonoids Extracts.
The preparation of solubility tea ball, comprises the steps:
(1) above-mentioned prepared Wild Pueraria flavone extract and Radix Glycyrrhizae are mixed, boiling 3 times, every time
2h, filters, merging filtrate；
(2) by filtrate concentration, lyophilization, prepare mixture, in mixture, add Mel, Mentholum
And cycloheptaamylose, mix homogeneously；
(3) homomixture being crossed 50 mesh sieves, the granule after sieving dispenses makes spherical tea ball in pill container；
(4) gained tea ball is crossed successively No. 3 compass screen surfaces and No. 3.5 sieve ends, be dried to moisture 2%, will be dried
After tea ball toast after, take out naturally cool to room temperature；
(5) the tea ball after cooling is screened out tiny foreign particle, obtain solubility tea ball, test package afterwards.
Embodiment 4
Radix Puerariae flavone purity testing in solubility tea ball:
Accurately weigh embodiment 1, embodiment 2, embodiment 3 extract the Kudzu Flavonoids Extracts 20mg that obtains,
It is placed in 250mL volumetric flask, adds 70% ethanol and dissolve and be diluted to scale, shake up, the most accurately draw
0.1,0.2,0.3,0.4,0.5,0.6,0.7,0.8,0.9,1.0 in 10mL volumetric flask, and respectively adds 70%
Ethanol 1.0mL, then add distilled water diluting and shake up to scale.Separately take 70% ethanol 1.0mL and put 10mL capacity
In Ping, add distilled water to scale, make blank, at 250nm wavelength, measure absorbance, according to standard
Curvilinear equation calculates the content of total flavones in sample solution, and then calculates Radix Puerariae flavone in thick Radix Puerariae flavone sample
Content, calculate Radix Puerariae flavone purity according to following equation.
Radix Puerariae flavone purity (%)=Radix Puerariae flavone weight (mg)/5 × 100
The mensuration of Radix Puerariae flavone antioxidant activity in solubility tea ball:
Example 1, embodiment 2, embodiment 3 extract the Radix Puerariae flavone extracting solution obtained and DPPH is free
The each 2.00ml of based sols adds in same tool plug test tube, shakes up.Reference is made with anhydrous ethanol solvent after 30min,
Measure its absorbance A i, measure 2.00mlDPPH free-atom aqueous solution and the mixed suction of 2.00ml solvent simultaneously
Luminosity A0, and 2.00ml flavonoids solution and mixed absorbance A j of 2.00ml anhydrous ethanol solvent, root
Calculating suppression ratio according to formula, antioxidant activity ability suppression ratio represents, suppression ratio is the biggest, antioxidant activity
The strongest.Its formula is: suppression ratio=1-(Ai-Aj)/A0 × 100% wherein, DPPH when Ai is for adding antioxidant
The absorbance of free-atom aqueous solution；Aj is that extracting solution is at the absorbance measuring wavelength；When A0 is not for adding antioxidant
The absorbance of DPPH free-atom aqueous solution, the antioxidant activity of Radix Puerariae flavone purity and Radix Puerariae flavone is shown in Table 1.
Radix Puerariae flavone purity and the antioxidant activity of Radix Puerariae flavone in table 1 solubility tea ball
As known from Table 1, the extractive technique purity of existing Radix Puerariae flavone is typically in 50-60%, the embodiment of the present invention
Kudzu Flavonoids Extracts purity in solubility tea ball can reach more than 90% and be far above prior art, and protects
Hold the high activity of flavone.
Claims (7)
1. one kind utilizes the method that Wild Pueraria flavone extract prepares solubility tea ball, it is characterised in that include as
Lower step:
(1) Wild Pueraria flavone extract and Radix Glycyrrhizae are mixed, boiling, filter, merging filtrate；
(2) filtrate is concentrated, lyophilization, prepare mixture, toward mixture in addition Mel, Mentholum and β-
Cyclodextrin, mix homogeneously；
(3) being sieved by homomixture, the granule after sieving dispenses makes spherical tea ball in pill container；
(4) gained tea ball is sieved successively, be dried, after being toasted by dried tea ball, take out natural cooling
To room temperature；
(5) the tea ball after cooling is screened out tiny foreign particle, obtain solubility tea ball, test package afterwards.
The most according to claim 1, utilize the method that Wild Pueraria flavone extract prepares solubility tea ball, it is special
Levy and be, the preparation method of described step (1) Wild Pueraria flavone extract, comprise the steps: open country
Raw Radix Puerariae lyophilization, pulverizes and sieves；Wild Pueraria powder after pulverizing adds the disodium hydrogen phosphate of pre-cooling in advance
-citrate buffer solution, adds compound enzyme and ferrous carbonate, at 15-20 DEG C of enzymolysis 40-60min, pH value 5.5-6.5；
After enzymolysis, enzymolysis solution is filtered, constant volume, obtain Radix Puerariae extracting solution, by extracting solution by ultramicrowave radiation treatment,
Then concentrating under reduced pressure, lyophilization prepare Kudzu Flavonoids Extracts.
The most according to claim 1, utilize the method that Wild Pueraria flavone extract prepares solubility tea ball, it is special
Levying and be, boiling 2 times-4 times in described step (1), each time is 1-3h.
The most according to claim 1, utilize the method that Wild Pueraria flavone extract prepares solubility tea ball, it is special
Levying and be, homomixture is crossed 40-60 mesh sieve by described step (3).
The most according to claim 1, utilize the method that Wild Pueraria flavone extract prepares solubility tea ball, it is special
Levying and be, gained tea ball is crossed and is sieved successively as spending No. 3.5 compass screen surfaces and No. 4 sieve ends or 3 by described step (4)
Number compass screen surface and No. 3.5 sieve ends sieves.
The most according to claim 1, utilize the method that Wild Pueraria flavone extract prepares solubility tea ball, it is special
Levying and be, described step (4) is dried to moisture below 5%.
7. utilize Wild Pueraria flavone extract to prepare obtained by the method for solubility tea ball as described in claim 1-6
Solubility tea ball, it is characterised in that described solubility tea ball, including made by the raw material of following parts by weight:
Kudzu Flavonoids Extracts 50-70 part, Radix Glycyrrhizae 20-40 part, Mel 5-15 part, Mentholum 7-12 part, β-ring
Shape dextrin 1-3 part.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106922906A (en) * | 2017-04-15 | 2017-07-07 | 申翰昭 | The preparation method of the thing of ball formula four drink life-nourishing tea bag and its health preserving tea of preparation |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106922906A (en) * | 2017-04-15 | 2017-07-07 | 申翰昭 | The preparation method of the thing of ball formula four drink life-nourishing tea bag and its health preserving tea of preparation |
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