CN101556273B - 利用电阻抗传感技术分析细胞迁移的方法及其专用装置 - Google Patents
利用电阻抗传感技术分析细胞迁移的方法及其专用装置 Download PDFInfo
- Publication number
- CN101556273B CN101556273B CN2008101035267A CN200810103526A CN101556273B CN 101556273 B CN101556273 B CN 101556273B CN 2008101035267 A CN2008101035267 A CN 2008101035267A CN 200810103526 A CN200810103526 A CN 200810103526A CN 101556273 B CN101556273 B CN 101556273B
- Authority
- CN
- China
- Prior art keywords
- electrode
- cell
- migration
- cell migration
- signal
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 230000012292 cell migration Effects 0.000 title claims abstract description 53
- 238000000034 method Methods 0.000 title claims abstract description 43
- 238000005516 engineering process Methods 0.000 title claims abstract description 22
- 238000007877 drug screening Methods 0.000 claims abstract description 4
- 238000011156 evaluation Methods 0.000 claims abstract description 4
- 210000004027 cell Anatomy 0.000 claims description 83
- 230000005012 migration Effects 0.000 claims description 18
- 238000013508 migration Methods 0.000 claims description 18
- 239000002094 self assembled monolayer Substances 0.000 claims description 16
- 239000013545 self-assembled monolayer Substances 0.000 claims description 16
- 238000004458 analytical method Methods 0.000 claims description 15
- 229910052751 metal Inorganic materials 0.000 claims description 15
- 239000002184 metal Substances 0.000 claims description 15
- 238000002847 impedance measurement Methods 0.000 claims description 14
- 239000010410 layer Substances 0.000 claims description 14
- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 claims description 14
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 claims description 11
- 229910052737 gold Inorganic materials 0.000 claims description 11
- 239000010931 gold Substances 0.000 claims description 11
- 229910052697 platinum Inorganic materials 0.000 claims description 7
- -1 sulfhydryl compound Chemical class 0.000 claims description 7
- 239000004372 Polyvinyl alcohol Substances 0.000 claims description 5
- 239000003814 drug Substances 0.000 claims description 5
- 239000002052 molecular layer Substances 0.000 claims description 5
- 235000019422 polyvinyl alcohol Nutrition 0.000 claims description 5
- 229920002451 polyvinyl alcohol Polymers 0.000 claims description 5
- 210000004748 cultured cell Anatomy 0.000 claims description 4
- 238000001338 self-assembly Methods 0.000 claims description 3
- 229940079593 drug Drugs 0.000 claims description 2
- 238000003745 diagnosis Methods 0.000 claims 1
- 201000010099 disease Diseases 0.000 claims 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims 1
- IAKHMKGGTNLKSZ-INIZCTEOSA-N (S)-colchicine Chemical compound C1([C@@H](NC(C)=O)CC2)=CC(=O)C(OC)=CC=C1C1=C2C=C(OC)C(OC)=C1OC IAKHMKGGTNLKSZ-INIZCTEOSA-N 0.000 description 14
- 238000001000 micrograph Methods 0.000 description 9
- 230000008569 process Effects 0.000 description 8
- 229960001338 colchicine Drugs 0.000 description 7
- 238000005259 measurement Methods 0.000 description 7
- 239000000758 substrate Substances 0.000 description 7
- 238000004113 cell culture Methods 0.000 description 6
- 238000012360 testing method Methods 0.000 description 5
- 239000011521 glass Substances 0.000 description 4
- 230000005764 inhibitory process Effects 0.000 description 4
- 238000012545 processing Methods 0.000 description 4
- 210000004881 tumor cell Anatomy 0.000 description 4
- 206010027476 Metastases Diseases 0.000 description 3
- 230000008901 benefit Effects 0.000 description 3
- 208000019065 cervical carcinoma Diseases 0.000 description 3
- 150000001875 compounds Chemical class 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 238000004520 electroporation Methods 0.000 description 3
- 210000002950 fibroblast Anatomy 0.000 description 3
- 238000000338 in vitro Methods 0.000 description 3
- 238000011081 inoculation Methods 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 2
- 206010020649 Hyperkeratosis Diseases 0.000 description 2
- 206010028980 Neoplasm Diseases 0.000 description 2
- 206010052428 Wound Diseases 0.000 description 2
- 208000027418 Wounds and injury Diseases 0.000 description 2
- 230000006907 apoptotic process Effects 0.000 description 2
- 239000013553 cell monolayer Substances 0.000 description 2
- 230000004663 cell proliferation Effects 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 230000003013 cytotoxicity Effects 0.000 description 2
- 231100000135 cytotoxicity Toxicity 0.000 description 2
- 230000005684 electric field Effects 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 230000004907 flux Effects 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 238000005192 partition Methods 0.000 description 2
- 238000001259 photo etching Methods 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 229920006395 saturated elastomer Polymers 0.000 description 2
- 238000013456 study Methods 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- 230000029663 wound healing Effects 0.000 description 2
- 208000037273 Pathologic Processes Diseases 0.000 description 1
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 1
- 230000000259 anti-tumor effect Effects 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 238000000429 assembly Methods 0.000 description 1
- 230000000712 assembly Effects 0.000 description 1
- 210000002457 barrier cell Anatomy 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000034196 cell chemotaxis Effects 0.000 description 1
- 239000006143 cell culture medium Substances 0.000 description 1
- 230000030833 cell death Effects 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 230000009087 cell motility Effects 0.000 description 1
- 230000036755 cellular response Effects 0.000 description 1
- 230000003399 chemotactic effect Effects 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000013020 embryo development Effects 0.000 description 1
- 230000009931 harmful effect Effects 0.000 description 1
- 230000028993 immune response Effects 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 230000002427 irreversible effect Effects 0.000 description 1
- 230000033001 locomotion Effects 0.000 description 1
- 238000001755 magnetron sputter deposition Methods 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 230000009054 pathological process Effects 0.000 description 1
- 230000002093 peripheral effect Effects 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 238000004445 quantitative analysis Methods 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 229910052710 silicon Inorganic materials 0.000 description 1
- 239000010703 silicon Substances 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
- 235000011149 sulphuric acid Nutrition 0.000 description 1
- 239000001117 sulphuric acid Substances 0.000 description 1
- 238000002560 therapeutic procedure Methods 0.000 description 1
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
- G01N33/502—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
- G01N33/5029—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects on cell motility
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/483—Physical analysis of biological material
- G01N33/4833—Physical analysis of biological material of solid biological material, e.g. tissue samples, cell cultures
- G01N33/4836—Physical analysis of biological material of solid biological material, e.g. tissue samples, cell cultures using multielectrode arrays
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54366—Apparatus specially adapted for solid-phase testing
- G01N33/54373—Apparatus specially adapted for solid-phase testing involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
- G01N33/5438—Electrodes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/10—Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/12—Specific details about manufacturing devices
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/06—Auxiliary integrated devices, integrated components
- B01L2300/0627—Sensor or part of a sensor is integrated
- B01L2300/0636—Integrated biosensor, microarrays
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/06—Auxiliary integrated devices, integrated components
- B01L2300/0627—Sensor or part of a sensor is integrated
- B01L2300/0645—Electrodes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/06—Auxiliary integrated devices, integrated components
- B01L2300/069—Absorbents; Gels to retain a fluid
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0809—Geometry, shape and general structure rectangular shaped
- B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/16—Surface properties and coatings
Abstract
Description
Claims (9)
Priority Applications (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN2008101035267A CN101556273B (zh) | 2008-04-08 | 2008-04-08 | 利用电阻抗传感技术分析细胞迁移的方法及其专用装置 |
US12/288,971 US8779779B2 (en) | 2008-04-08 | 2008-10-24 | On-chip cell migration detection |
EP08169204A EP2108955B1 (en) | 2008-04-08 | 2008-11-14 | On-Chip Cell Migration Detection |
AT08169204T ATE550652T1 (de) | 2008-04-08 | 2008-11-14 | On-chip-zellenmigrationserkennung |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN2008101035267A CN101556273B (zh) | 2008-04-08 | 2008-04-08 | 利用电阻抗传感技术分析细胞迁移的方法及其专用装置 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN101556273A CN101556273A (zh) | 2009-10-14 |
CN101556273B true CN101556273B (zh) | 2013-03-20 |
Family
ID=40263378
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN2008101035267A Active CN101556273B (zh) | 2008-04-08 | 2008-04-08 | 利用电阻抗传感技术分析细胞迁移的方法及其专用装置 |
Country Status (4)
Country | Link |
---|---|
US (1) | US8779779B2 (zh) |
EP (1) | EP2108955B1 (zh) |
CN (1) | CN101556273B (zh) |
AT (1) | ATE550652T1 (zh) |
Families Citing this family (22)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2005027714A2 (en) | 2003-07-12 | 2005-03-31 | Accelr8 Technology Corporation | Sensitive and rapid biodetection |
US20120077206A1 (en) | 2003-07-12 | 2012-03-29 | Accelr8 Technology Corporation | Rapid Microbial Detection and Antimicrobial Susceptibility Testing |
CN101038284B (zh) * | 2007-04-25 | 2011-04-27 | 博奥生物有限公司 | 一种提高电阻抗检测装置的电阻抗检测灵敏度的方法 |
CN101614729B (zh) * | 2008-06-27 | 2013-04-24 | 博奥生物有限公司 | 用于细胞操作及电生理信号检测的微电极阵列器件及专用装置 |
US8421481B2 (en) * | 2009-10-20 | 2013-04-16 | Analog Devices, Inc. | Detection and mitigation of particle contaminants in MEMS devices |
US10254204B2 (en) | 2011-03-07 | 2019-04-09 | Accelerate Diagnostics, Inc. | Membrane-assisted purification |
WO2012122314A2 (en) | 2011-03-07 | 2012-09-13 | Accelr8 Technology Corporation | Rapid cell purification systems |
US9044611B2 (en) * | 2013-03-15 | 2015-06-02 | The Regents Of The University Of California | Systems and methods for selectively migrating cells using electric fields |
US9677109B2 (en) | 2013-03-15 | 2017-06-13 | Accelerate Diagnostics, Inc. | Rapid determination of microbial growth and antimicrobial susceptibility |
US9857370B2 (en) | 2013-07-22 | 2018-01-02 | National Technology & Engineering Solutions Of Sandia, Llc | Amplification of biological targets via on-chip culture for biosensing |
US9957554B1 (en) | 2013-12-19 | 2018-05-01 | National Technology & Engineering Solutions Of Sandia, Llc | Microfluidic platform for multiplexed detection in single cells and methods thereof |
US9995411B1 (en) | 2014-07-16 | 2018-06-12 | National Technology & Engineering Solutions Of Sandia, Llc | High-temperature, adhesive-based microvalves and uses thereof |
US10023895B2 (en) | 2015-03-30 | 2018-07-17 | Accelerate Diagnostics, Inc. | Instrument and system for rapid microogranism identification and antimicrobial agent susceptibility testing |
US10253355B2 (en) | 2015-03-30 | 2019-04-09 | Accelerate Diagnostics, Inc. | Instrument and system for rapid microorganism identification and antimicrobial agent susceptibility testing |
WO2017003553A1 (en) * | 2015-06-30 | 2017-01-05 | GM Global Technology Operations LLC | Method of determining deformation location |
US10874324B2 (en) | 2015-10-20 | 2020-12-29 | General Electric Company | Detection of physiological changes in impedance monitoring |
CN105255703B (zh) * | 2015-10-21 | 2018-02-23 | 中国科学院电子学研究所 | 用于大序列细胞划痕迁移观测的微流控芯片及其观测方法 |
US10613088B2 (en) * | 2016-06-12 | 2020-04-07 | Mohammad Abdolahad | Method and system for metastasis diagnosis and prognosis |
EP3299804A1 (de) * | 2016-09-27 | 2018-03-28 | Georg Fischer JRG AG | Verfahren und vorrichtung zur analyse der bakteriendichte in trinkwasser |
CN110187091B (zh) * | 2019-04-18 | 2020-11-27 | 浙江大学 | 一种用于抗肿瘤药物筛选的高通量3d细胞阻抗传感器及检测方法 |
CN110711608B (zh) * | 2019-09-23 | 2022-03-15 | 中国科学院上海微***与信息技术研究所 | 一种用于细胞检测的微流控芯片及其制备方法 |
US20230051840A1 (en) * | 2020-01-21 | 2023-02-16 | Synthego Corporation | Devices and methods for transfection and for generation of clonal populations of cells |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1628863A (zh) * | 2004-10-14 | 2005-06-22 | 大连理工大学 | 一种心血管支架聚合物载药涂层制备方法 |
Family Cites Families (34)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3513384A (en) | 1966-05-20 | 1970-05-19 | Carl A Schneider | Electrolytic conductivity cell having unplatinized metal electrodes |
US4920047A (en) | 1988-05-20 | 1990-04-24 | General Electric Company | Electrical detection of the immune reaction |
US5187096A (en) | 1991-08-08 | 1993-02-16 | Rensselaer Polytechnic Institute | Cell substrate electrical impedance sensor with multiple electrode array |
US5514501A (en) | 1994-06-07 | 1996-05-07 | The United States Of America As Represented By The Secretary Of Commerce | Process for UV-photopatterning of thiolate monolayers self-assembled on gold, silver and other substrates |
US5523878A (en) | 1994-06-30 | 1996-06-04 | Texas Instruments Incorporated | Self-assembled monolayer coating for micro-mechanical devices |
US5942443A (en) | 1996-06-28 | 1999-08-24 | Caliper Technologies Corporation | High throughput screening assay systems in microscale fluidic devices |
US7462324B2 (en) | 1997-08-07 | 2008-12-09 | Panasonic Corporation | Measurement device and method for measuring electric signal from biological sample |
US6758961B1 (en) | 1997-12-17 | 2004-07-06 | Ecole Polytechnique Federale De Lausanne | Positioning and electrophysiological characterization of individual cells and reconstituted membrane systems on microstructured carriers |
US6942771B1 (en) | 1999-04-21 | 2005-09-13 | Clinical Micro Sensors, Inc. | Microfluidic systems in the electrochemical detection of target analytes |
US6645359B1 (en) * | 2000-10-06 | 2003-11-11 | Roche Diagnostics Corporation | Biosensor |
US6437551B1 (en) | 1999-11-02 | 2002-08-20 | The Regents Of The University Of California | Microfabricated AC impedance sensor |
US20050009004A1 (en) | 2002-05-04 | 2005-01-13 | Jia Xu | Apparatus including ion transport detecting structures and methods of use |
US7332313B2 (en) * | 2001-06-05 | 2008-02-19 | Applied Biophysics, Inc. | Electrical wounding assay for cells in vitro |
CA2482629A1 (en) | 2002-04-18 | 2003-10-30 | University Of South Florida | Global equivalent circuit modeling system for substrate mounted circuit components incorporating substrate dependent characteristics |
US7560269B2 (en) | 2002-12-20 | 2009-07-14 | Acea Biosciences, Inc. | Real time electronic cell sensing system and applications for cytotoxicity profiling and compound assays |
US8206903B2 (en) | 2002-12-20 | 2012-06-26 | Acea Biosciences | Device and method for electroporation-based delivery of molecules into cells and dynamic monitoring of cell responses |
JP4745056B2 (ja) | 2002-07-20 | 2011-08-10 | アセア バイオサイエンシーズ,インク. | インピーダンスによる細胞と微粒子の測定装置及び方法 |
WO2005047482A2 (en) | 2003-11-12 | 2005-05-26 | Xiao Xu | Real time electronic cell sensing systems and applications for cell-based assays |
GB0223666D0 (en) * | 2002-10-10 | 2002-11-20 | Univ Cambridge Tech | Arrays of molecules and their production |
US20050003396A1 (en) | 2003-04-11 | 2005-01-06 | Mihrimah Ozkan | Biosensors having single reactant components immobilized over single electrodes and methods of making and using thereof |
US7399631B2 (en) | 2003-09-10 | 2008-07-15 | Applied Biophysics, Inc. | Real-time impedance assay to follow the invasive activities of metastatic cells in culture |
US7547381B2 (en) | 2003-09-26 | 2009-06-16 | Agency For Science, Technology And Research And National University Of Singapore | Sensor array integrated electrochemical chip, method of forming same, and electrode coating |
US8423114B2 (en) * | 2006-10-04 | 2013-04-16 | Dexcom, Inc. | Dual electrode system for a continuous analyte sensor |
ATE455180T1 (de) | 2004-01-29 | 2010-01-15 | Narvalus S R L | Biochip electroporationsgerät mit mehreren positionen zur einzelzellelectroporation |
SG151334A1 (en) * | 2004-04-01 | 2009-04-30 | Univ Nanyang | Addressable chem/bio chip array |
US20060105321A1 (en) | 2004-07-28 | 2006-05-18 | The University Of Iowa Research Foundation | Methods for evaluating cell membrane properties |
CN1996001A (zh) | 2006-01-06 | 2007-07-11 | 博奥生物有限公司 | 监测细胞和生物分子生物学行为的生物传感器及传感方法 |
JP4735833B2 (ja) * | 2006-01-13 | 2011-07-27 | セイコーエプソン株式会社 | バイオチップ及びバイオセンサ |
KR100748722B1 (ko) | 2006-04-03 | 2007-08-13 | 삼성전자주식회사 | 미소소자 패키지 모듈 및 그 제조방법 |
US7323892B1 (en) | 2006-06-07 | 2008-01-29 | Agilent Technologies, Inc. | Probe having a frame to align spring pins perpendicularly to a printed circuit board, and method of making same |
US7534968B2 (en) | 2006-11-03 | 2009-05-19 | Laird Technologies, Inc. | Snap install EMI shields with protrusions and electrically-conductive members for attachment to substrates |
TW200830963A (en) | 2007-01-09 | 2008-07-16 | Inventec Corp | Welding clamp |
CN101038284B (zh) | 2007-04-25 | 2011-04-27 | 博奥生物有限公司 | 一种提高电阻抗检测装置的电阻抗检测灵敏度的方法 |
CN101614729B (zh) | 2008-06-27 | 2013-04-24 | 博奥生物有限公司 | 用于细胞操作及电生理信号检测的微电极阵列器件及专用装置 |
-
2008
- 2008-04-08 CN CN2008101035267A patent/CN101556273B/zh active Active
- 2008-10-24 US US12/288,971 patent/US8779779B2/en active Active
- 2008-11-14 AT AT08169204T patent/ATE550652T1/de active
- 2008-11-14 EP EP08169204A patent/EP2108955B1/en active Active
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1628863A (zh) * | 2004-10-14 | 2005-06-22 | 大连理工大学 | 一种心血管支架聚合物载药涂层制备方法 |
Non-Patent Citations (2)
Title |
---|
IVAR GIAEVER AND CHARLES R. KEESE.Micromotion of mammalian cells measured electrically.《Proc. Nail. Acad. Sci. USA》.1991,第88卷7896-7900. * |
张清楠.血管平滑肌细胞在高糖诱导下增殖迁移过程的监测及应用.《中国优秀硕士学位论文全文数据库(医药卫生科技辑)》.2006,(第9期),E065-57. * |
Also Published As
Publication number | Publication date |
---|---|
EP2108955A3 (en) | 2010-05-19 |
EP2108955A2 (en) | 2009-10-14 |
US8779779B2 (en) | 2014-07-15 |
EP2108955B1 (en) | 2012-03-21 |
US20090251155A1 (en) | 2009-10-08 |
ATE550652T1 (de) | 2012-04-15 |
CN101556273A (zh) | 2009-10-14 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN101556273B (zh) | 利用电阻抗传感技术分析细胞迁移的方法及其专用装置 | |
Hierlemann et al. | Growing cells atop microelectronic chips: interfacing electrogenic cells in vitro with CMOS-based microelectrode arrays | |
Xie et al. | Intracellular recording of action potentials by nanopillar electroporation | |
CN102382758B (zh) | 基于细胞打印和多参数传感阵列集成技术的三维细胞芯片 | |
Merz et al. | Silicon chip interfaced with a geometrically defined net of snail neurons | |
EP1464952B1 (en) | Apparatus for stimulating an animal cell and recording its physiological signal and production and use methods thereof | |
US10591462B2 (en) | Electrochemical method and device for detecting the effect of anticancer drugs | |
US8722393B2 (en) | Apparatus for facilitating evaluating migration of cells in vitro | |
CN113941378B (zh) | 基于多腔式电生理微纳检测的神经类器官芯片及检测方法 | |
JPH06296595A (ja) | 一体化複合電極 | |
KR20090099767A (ko) | 셀센서 및 이를 이용하여 세포의 상태를 실시간으로모니터링하는 방법 | |
Mohr et al. | Performance of a thin film microelectrode array for monitoring electrogenic cells in vitro | |
Wolf et al. | Microsensor-aided measurements of cellular signalling and metabolism on tumor cells: the cell monitoring system (CMS®) | |
US20200055041A1 (en) | Device, system and methods for electrophysiological interrogation of cells and tissues | |
Zhang et al. | Long-term and label-free monitoring for osteogenic differentiation of mesenchymal stem cells using force sensor and impedance measurement | |
Yadav et al. | Development of multi-depth probing 3D microelectrode array to record electrophysiological activity within neural cultures | |
CN107354087B (zh) | 一种基于可降解生物传感器的心肌细胞生长状态监控*** | |
US20210199638A1 (en) | Sensor array and apparatus for simultaneous observation of tissue electrophysiology, contractility, and growth | |
CN102337211B (zh) | 细胞培养装置 | |
Prendecka et al. | Electric Cell Substrate Impedance Sensing (ECIS) as a unique technique in cancer metastasis research | |
Das et al. | Electrical characterization of suspended HeLa cells using ECIS based biosensor | |
US11536684B2 (en) | Electrochemical method and device for detecting the effect of anticancer drugs | |
Gómez et al. | Microelectrode array platform for cell monitoring signal acquisition system | |
CN109596818B (zh) | 一种基于电生理学分析当归四逆汤预防奥沙利铂神经毒性机制的研究方法 | |
Ferrario et al. | Electrochemical impedance spectroscopy study of the cells adhesion over microelectrodes array |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
C14 | Grant of patent or utility model | ||
GR01 | Patent grant | ||
C56 | Change in the name or address of the patentee |
Owner name: CAPITALBIO CORPORATION CO., LTD. Free format text: FORMER NAME: CAPITALBIO CORPORATION |
|
CP01 | Change in the name or title of a patent holder |
Address after: 102206 Beijing City, Changping District Life Science Park Road No. 18 Patentee after: CAPITALBIO CORPORATION Patentee after: Tsinghua University Address before: 102206 Beijing City, Changping District Life Science Park Road No. 18 Patentee before: Capitalbio Corporation Patentee before: Tsinghua University |