CA2198489A1 - Biodetecteur et methode de detection chimioluminescente a electrogeneration pour l'acide nudeique adsorbe sur une surface solide - Google Patents

Biodetecteur et methode de detection chimioluminescente a electrogeneration pour l'acide nudeique adsorbe sur une surface solide

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Publication number
CA2198489A1
CA2198489A1 CA002198489A CA2198489A CA2198489A1 CA 2198489 A1 CA2198489 A1 CA 2198489A1 CA 002198489 A CA002198489 A CA 002198489A CA 2198489 A CA2198489 A CA 2198489A CA 2198489 A1 CA2198489 A1 CA 2198489A1
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CA
Canada
Prior art keywords
dna
nucleic acid
film
electrode
label
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002198489A
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English (en)
Inventor
Allen J. Bard
Xiao-Hong Xu
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IGEN International Inc
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Individual
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Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2198489A1 publication Critical patent/CA2198489A1/fr
Abandoned legal-status Critical Current

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/001—Enzyme electrodes
    • C12Q1/005—Enzyme electrodes involving specific analytes or enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6825—Nucleic acid detection involving sensors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)

Abstract

De l'ADN simple brin a été immobilisé sur une électrode recouverte d'une pellicule d'alcanebisphosphonate d'aluminium par immersion de cette électrode dans une solution d'ADN simple brin. Cet ADN simple brin immobilisé, marqué par Ru(bpy)¿3??2+¿, a été détecté par surveillance de la luminescence électrophotochimique (ECL) produite suite à l'oxydation dans une solution contenant de la tri-n-propylamine. Après l'immobilisation d'ADN simple brin non marqué, un brin d'ADN complémentaire marqué a été hybridé pour produire de l'ADN double brin sur la surface. L'étendue de l'hybridation d'ADN a été déterminée par la luminescence ECL de Ru(bpy)¿3??2+¿ marqué. L'ADN double brin immobilisé sur une surface pourrait également être détecté par observation de la luminescence ECL de Ru(phen)¿3??2+¿. La microscopie électronique à transmission (TEM) a été utilisée pour obtenir une image de la pellicule et de l'ADN immobilisé.
CA002198489A 1994-08-26 1995-08-25 Biodetecteur et methode de detection chimioluminescente a electrogeneration pour l'acide nudeique adsorbe sur une surface solide Abandoned CA2198489A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US29663094A 1994-08-26 1994-08-26
US08/296,630 1994-08-26

Publications (1)

Publication Number Publication Date
CA2198489A1 true CA2198489A1 (fr) 1996-03-07

Family

ID=23142843

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002198489A Abandoned CA2198489A1 (fr) 1994-08-26 1995-08-25 Biodetecteur et methode de detection chimioluminescente a electrogeneration pour l'acide nudeique adsorbe sur une surface solide

Country Status (5)

Country Link
EP (1) EP0777741A4 (fr)
JP (1) JPH10509025A (fr)
AU (1) AU703344B2 (fr)
CA (1) CA2198489A1 (fr)
WO (1) WO1996006946A1 (fr)

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US5786141A (en) * 1994-08-26 1998-07-28 Bard; Allen J. Electrogenerated chemiluminescence labels for analysis and/or referencing
US6066448A (en) * 1995-03-10 2000-05-23 Meso Sclae Technologies, Llc. Multi-array, multi-specific electrochemiluminescence testing
US6207369B1 (en) 1995-03-10 2001-03-27 Meso Scale Technologies, Llc Multi-array, multi-specific electrochemiluminescence testing
US6140045A (en) 1995-03-10 2000-10-31 Meso Scale Technologies Multi-array, multi-specific electrochemiluminescence testing
US6673533B1 (en) 1995-03-10 2004-01-06 Meso Scale Technologies, Llc. Multi-array multi-specific electrochemiluminescence testing
US6319670B1 (en) 1995-05-09 2001-11-20 Meso Scale Technology Llp Methods and apparatus for improved luminescence assays using microparticles
US7014992B1 (en) 1996-11-05 2006-03-21 Clinical Micro Sensors, Inc. Conductive oligomers attached to electrodes and nucleoside analogs
FR2762013B1 (fr) 1997-04-09 1999-06-04 Cis Bio Int Systemes de detection d'hybridation d'acides nucleiques, leur procede de preparation et leurs utilisations
US6289229B1 (en) 1998-01-20 2001-09-11 Scimed Life Systems, Inc. Readable probe array for in vivo use
US6942771B1 (en) 1999-04-21 2005-09-13 Clinical Micro Sensors, Inc. Microfluidic systems in the electrochemical detection of target analytes
EP1218541B1 (fr) 1999-07-26 2008-12-10 Clinical Micro Sensors, Inc. Determination de sequences d'acides nucleiques par detection electronique
AU2907201A (en) * 1999-12-09 2001-06-18 Motorola, Inc. Methods and compositions relating to electrical detection of nucleic acid reactions
US6518024B2 (en) 1999-12-13 2003-02-11 Motorola, Inc. Electrochemical detection of single base extension
US6824669B1 (en) 2000-02-17 2004-11-30 Motorola, Inc. Protein and peptide sensors using electrical detection methods
US6706479B2 (en) * 2000-10-05 2004-03-16 Virginia Tech Intellectual Properties, Inc. Bio-chip, photoluminescent methods for identifying biological material, and apparatuses for use with such methods and bio-chips
WO2005016115A2 (fr) 2003-01-23 2005-02-24 Montana State University Biocapteurs faisant intervenir des ligands immobilises sur des dendrimeres et utilisation de ceux-ci
ATE445715T1 (de) 2003-05-13 2009-10-15 Trustees Boston College Elektrokatalytischer nukleinsäurehybridisierungsnachweis
US7741033B2 (en) * 2003-05-13 2010-06-22 Trustees Of Boston College Electrocatalytic nucleic acid hybridization detection
WO2006075497A1 (fr) * 2004-12-21 2006-07-20 Kyoto University Sonde, dispositif d'identification de region nucleotidique et procede d'identification de region nucleotidique
ES2517919T3 (es) 2008-09-02 2014-11-04 The Governing Council Of The University Of Toronto Microelectrodos nanoestructurados y dispositivos de biodetección que los incorporan
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EP2683822B1 (fr) 2011-03-10 2020-04-22 General Atomics Dispositifs et méthodes de diagnostic et de préparation d'échantillons
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WO2017153336A1 (fr) 2016-03-07 2017-09-14 F. Hoffmann-La Roche Ag Détection d'anticorps anti-p53
WO2018141768A1 (fr) 2017-02-02 2018-08-09 Roche Diagnostics Gmbh Dosage immunologique utilisant au moins deux agents de liaison spécifiques à un analyte pegylés
CN111936522A (zh) 2018-04-18 2020-11-13 豪夫迈·罗氏有限公司 新型抗胸苷激酶抗体
WO2020043868A1 (fr) 2018-08-31 2020-03-05 F. Hoffmann-La Roche Ag Thymidine kinase (tk-1) dans la détermination d'indices de pronostic pour le dlbcl
KR20220024699A (ko) 2019-07-22 2022-03-03 에프. 호프만-라 로슈 아게 자궁내막증의 비침습적 진단을 위한 혈액 바이오마커로서의 s100a12
CN114144674A (zh) 2019-07-22 2022-03-04 豪夫迈·罗氏有限公司 P物质作为用于子宫内膜异位症的非侵入性诊断的血液生物标志物
WO2021013784A1 (fr) 2019-07-22 2021-01-28 F. Hoffmann-La Roche Ag S100a8 utilisée en tant que biomarqueur sanguin pour le diagnostic non invasif de l'endométriose
CN114144675A (zh) 2019-07-22 2022-03-04 豪夫迈·罗氏有限公司 S100a9作为血液生物标志物用于子宫内膜异位症的非侵入性诊断
EP4004555A1 (fr) 2019-07-22 2022-06-01 F. Hoffmann-La Roche AG S100a6 en tant que biomarqueur du sang pour le diagnostic non invasif de l'endométriose
WO2021094409A1 (fr) 2019-11-15 2021-05-20 F. Hoffmann-La Roche Ag Dérivation d'antibiotiques de bêta-lactame pour des mesures par spectrométrie de masse dans des échantillons de patients
CN111272742B (zh) * 2020-03-06 2022-11-08 安徽大学 基于金属有机凝胶复合材料和金属有机框架的电致化学发光传感器及其制备和检测方法
WO2022090455A1 (fr) 2020-11-02 2022-05-05 F. Hoffmann-La Roche Ag Anticorps nucléocapsidiques du sars-cov-2
WO2022207628A1 (fr) 2021-03-30 2022-10-06 F. Hoffmann-La Roche Ag Scf comme biomarqueur sanguin pour le diagnostic non invasif de l'endométriose
CN117099001A (zh) 2021-04-01 2023-11-21 豪夫迈·罗氏有限公司 Psp94作为血液生物标志物用于子宫内膜异位症的非侵入性诊断
JP2024518180A (ja) 2021-05-17 2024-04-25 エフ． ホフマン－ラ ロシュ アーゲー 腺筋症の非侵襲的診断のための血液バイオマーカーとしてのｓＦＲＰ４
WO2023072904A1 (fr) 2021-10-26 2023-05-04 F. Hoffmann-La Roche Ag Anticorps monoclonaux spécifiques du rbd du sars-cov-2
WO2023131594A1 (fr) 2022-01-05 2023-07-13 F. Hoffmann-La Roche Ag Dérivatisation de composés dans des échantillons de patients pour la pharmacovigilance thérapeutique (tdm)
WO2023247752A1 (fr) 2022-06-23 2023-12-28 F. Hoffmann-La Roche Ag Procédé de diagnostic de l'endométriose et de classification du stade de l'endométriose
WO2024017983A1 (fr) 2022-07-22 2024-01-25 F. Hoffmann-La Roche Ag Protéine de type météorine (metrnl) utilisée en tant que biomarqueur (sang) pour le diagnostic du syndrome des ovaires polykystiques
WO2024017985A1 (fr) 2022-07-22 2024-01-25 F. Hoffmann-La Roche Ag Protéine 1 de liaison au facteur de croissance des fibroblastes (fgfbp1) utilisée en tant que biomarqueur (sanguin) pour le diagnostic du syndrome des ovaires polykystiques
WO2024017982A1 (fr) 2022-07-22 2024-01-25 F. Hoffmann-La Roche Ag Leukotriène a4 hydrolase (lta4h) en tant que biomarqueur (sang) pour le diagnostic du syndrome des ovaires polykystiques
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Also Published As

Publication number Publication date
AU3410395A (en) 1996-03-22
JPH10509025A (ja) 1998-09-08
AU703344B2 (en) 1999-03-25
EP0777741A1 (fr) 1997-06-11
EP0777741A4 (fr) 1999-01-13
WO1996006946A1 (fr) 1996-03-07

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