BR112014015417A2 - diferenciação de células-tronco embrionárias humanas em células positivas de insulina hormonal únicas - Google Patents

diferenciação de células-tronco embrionárias humanas em células positivas de insulina hormonal únicas

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Publication number
BR112014015417A2
BR112014015417A2 BR112014015417A BR112014015417A BR112014015417A2 BR 112014015417 A2 BR112014015417 A2 BR 112014015417A2 BR 112014015417 A BR112014015417 A BR 112014015417A BR 112014015417 A BR112014015417 A BR 112014015417A BR 112014015417 A2 BR112014015417 A2 BR 112014015417A2
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BR
Brazil
Prior art keywords
cells
differentiation
stem cells
human embryonic
embryonic stem
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BR112014015417A
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English (en)
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BR112014015417A8 (pt
Inventor
Rezania Alireza
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Janssen Biotech Inc
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Publication date
Application filed by Janssen Biotech Inc filed Critical Janssen Biotech Inc
Publication of BR112014015417A2 publication Critical patent/BR112014015417A2/pt
Publication of BR112014015417A8 publication Critical patent/BR112014015417A8/pt

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    • C—CHEMISTRY; METALLURGY
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    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0676—Pancreatic cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/34—Sugars
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/38—Vitamins
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/117—Keratinocyte growth factors (KGF-1, i.e. FGF-7; KGF-2, i.e. FGF-12)
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/15—Transforming growth factor beta (TGF-β)
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/155—Bone morphogenic proteins [BMP]; Osteogenins; Osteogenic factor; Bone inducing factor
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/16—Activin; Inhibin; Mullerian inhibiting substance
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/19—Growth and differentiation factors [GDF]
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/38—Hormones with nuclear receptors
    • C12N2501/385—Hormones with nuclear receptors of the family of the retinoic acid recptor, e.g. RAR, RXR; Peroxisome proliferator-activated receptor [PPAR]
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/40—Regulators of development
    • C12N2501/41—Hedgehog proteins; Cyclopamine (inhibitor)
    • C—CHEMISTRY; METALLURGY
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/40—Regulators of development
    • C12N2501/415—Wnt; Frizzeled
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70—Enzymes
    • C12N2501/72—Transferases (EC 2.)
    • C12N2501/727—Kinases (EC 2.7.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells

Abstract

abstract the present invention provides methods to promote the differentiation of pluripotent stem cells. in particular, the present invention provides methods to produce a population of cells, wherein greater than 10% of the cells in the population express markers characteristic of single hormonal pancreatic beta cells. tradução do resumo resumo patente de invenção "diferenciação de células-tronco embrionárias humanas em células positivas de insulina hormonal únicas" a presente invenção apresenta métodos para promover a diferenciação de células-tronco pluripotentes. em particular, a presente invenção fornece métodos para produzir uma população de células, em que mais do que 10% das células na população expressa marcadores característicos de células pancreáticas beta hormonais únicas.
BR112014015417A 2011-12-22 2012-12-07 diferenciação de células-tronco embrionárias humanas em células positivas de insulina hormonal únicas BR112014015417A8 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201161579351P 2011-12-22 2011-12-22
PCT/US2012/068439 WO2013095953A1 (en) 2011-12-22 2012-12-07 Differentiation of human embryonic stem cells into single hormonal insulin positive cells

Publications (2)

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BR112014015417A2 true BR112014015417A2 (pt) 2017-06-13
BR112014015417A8 BR112014015417A8 (pt) 2017-07-04

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Country Status (15)

Country Link
US (4) US9388386B2 (pt)
EP (1) EP2794857A4 (pt)
JP (4) JP6441080B2 (pt)
KR (2) KR102203056B1 (pt)
CN (1) CN105143446B (pt)
AR (1) AR089426A1 (pt)
AU (3) AU2012355698B2 (pt)
BR (1) BR112014015417A8 (pt)
CA (1) CA2860107C (pt)
HK (1) HK1203552A1 (pt)
MX (1) MX2014007744A (pt)
PH (1) PH12014501429A1 (pt)
RU (2) RU2705001C2 (pt)
SG (2) SG11201403473QA (pt)
WO (1) WO2013095953A1 (pt)

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