WO2024109914A1 - 一种包含特异性结合hgfr和egfr的双特异性抗体的药物组合物 - Google Patents
一种包含特异性结合hgfr和egfr的双特异性抗体的药物组合物 Download PDFInfo
- Publication number
- WO2024109914A1 WO2024109914A1 PCT/CN2023/133895 CN2023133895W WO2024109914A1 WO 2024109914 A1 WO2024109914 A1 WO 2024109914A1 CN 2023133895 W CN2023133895 W CN 2023133895W WO 2024109914 A1 WO2024109914 A1 WO 2024109914A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- seq
- pharmaceutical composition
- hgfr
- egfr
- specifically binds
- Prior art date
Links
- 239000008194 pharmaceutical composition Substances 0.000 title claims abstract description 259
- 230000027455 binding Effects 0.000 title claims abstract description 103
- 108060006698 EGF receptor Proteins 0.000 title description 213
- 101000972946 Homo sapiens Hepatocyte growth factor receptor Proteins 0.000 claims abstract description 29
- 102100022623 Hepatocyte growth factor receptor Human genes 0.000 claims abstract 26
- 102000052116 epidermal growth factor receptor activity proteins Human genes 0.000 claims abstract 26
- 108700015053 epidermal growth factor receptor activity proteins Proteins 0.000 claims abstract 26
- YOHYSYJDKVYCJI-UHFFFAOYSA-N n-[3-[[6-[3-(trifluoromethyl)anilino]pyrimidin-4-yl]amino]phenyl]cyclopropanecarboxamide Chemical compound FC(F)(F)C1=CC=CC(NC=2N=CN=C(NC=3C=C(NC(=O)C4CC4)C=CC=3)C=2)=C1 YOHYSYJDKVYCJI-UHFFFAOYSA-N 0.000 claims abstract 26
- 239000000872 buffer Substances 0.000 claims description 170
- 239000000243 solution Substances 0.000 claims description 124
- 229930006000 Sucrose Natural products 0.000 claims description 110
- 239000000244 polyoxyethylene sorbitan monooleate Substances 0.000 claims description 110
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 claims description 110
- 229920000053 polysorbate 80 Polymers 0.000 claims description 110
- 229940068968 polysorbate 80 Drugs 0.000 claims description 110
- 239000005720 sucrose Substances 0.000 claims description 110
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 105
- 102000036639 antigens Human genes 0.000 claims description 86
- 108091007433 antigens Proteins 0.000 claims description 86
- 239000000427 antigen Substances 0.000 claims description 84
- 238000002360 preparation method Methods 0.000 claims description 84
- 150000001413 amino acids Chemical group 0.000 claims description 71
- 235000000346 sugar Nutrition 0.000 claims description 60
- 239000004094 surface-active agent Substances 0.000 claims description 49
- 229930182817 methionine Natural products 0.000 claims description 41
- 239000000546 pharmaceutical excipient Substances 0.000 claims description 40
- 102000004190 Enzymes Human genes 0.000 claims description 37
- 108090000790 Enzymes Proteins 0.000 claims description 37
- 229940088598 enzyme Drugs 0.000 claims description 37
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 claims description 35
- 238000004108 freeze drying Methods 0.000 claims description 34
- 206010028980 Neoplasm Diseases 0.000 claims description 29
- ODKSFYDXXFIFQN-BYPYZUCNSA-N L-arginine Chemical compound OC(=O)[C@@H](N)CCCN=C(N)N ODKSFYDXXFIFQN-BYPYZUCNSA-N 0.000 claims description 28
- 239000008351 acetate buffer Substances 0.000 claims description 28
- 229960003589 arginine hydrochloride Drugs 0.000 claims description 28
- 108010003272 Hyaluronate lyase Proteins 0.000 claims description 27
- 102000001974 Hyaluronidases Human genes 0.000 claims description 27
- 229960002773 hyaluronidase Drugs 0.000 claims description 27
- HNDVDQJCIGZPNO-UHFFFAOYSA-N histidine Natural products OC(=O)C(N)CC1=CN=CN1 HNDVDQJCIGZPNO-UHFFFAOYSA-N 0.000 claims description 26
- 238000000034 method Methods 0.000 claims description 24
- NFGXHKASABOEEW-UHFFFAOYSA-N 1-methylethyl 11-methoxy-3,7,11-trimethyl-2,4-dodecadienoate Chemical compound COC(C)(C)CCCC(C)CC=CC(C)=CC(=O)OC(C)C NFGXHKASABOEEW-UHFFFAOYSA-N 0.000 claims description 13
- 201000010099 disease Diseases 0.000 claims description 13
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims description 13
- 208000020816 lung neoplasm Diseases 0.000 claims description 10
- 239000007929 subcutaneous injection Substances 0.000 claims description 10
- 238000010254 subcutaneous injection Methods 0.000 claims description 10
- 206010058467 Lung neoplasm malignant Diseases 0.000 claims description 9
- 238000010253 intravenous injection Methods 0.000 claims description 9
- 201000005202 lung cancer Diseases 0.000 claims description 9
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 7
- 206010009944 Colon cancer Diseases 0.000 claims description 6
- 208000005718 Stomach Neoplasms Diseases 0.000 claims description 6
- 239000007979 citrate buffer Substances 0.000 claims description 6
- 206010017758 gastric cancer Diseases 0.000 claims description 6
- 239000007928 intraperitoneal injection Substances 0.000 claims description 6
- 125000001360 methionine group Chemical group N[C@@H](CCSC)C(=O)* 0.000 claims description 6
- 239000008363 phosphate buffer Substances 0.000 claims description 6
- 201000011549 stomach cancer Diseases 0.000 claims description 6
- 239000008362 succinate buffer Substances 0.000 claims description 6
- HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 claims description 5
- HNDVDQJCIGZPNO-YFKPBYRVSA-N L-histidine Chemical compound OC(=O)[C@@H](N)CC1=CN=CN1 HNDVDQJCIGZPNO-YFKPBYRVSA-N 0.000 claims description 5
- HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 claims description 5
- HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 claims description 5
- 201000010536 head and neck cancer Diseases 0.000 claims description 5
- 208000014829 head and neck neoplasm Diseases 0.000 claims description 5
- 229920001983 poloxamer Polymers 0.000 claims description 5
- 125000000185 sucrose group Chemical group 0.000 claims description 5
- 208000001333 Colorectal Neoplasms Diseases 0.000 claims description 4
- FBPFZTCFMRRESA-FSIIMWSLSA-N D-Glucitol Natural products OC[C@H](O)[C@H](O)[C@@H](O)[C@H](O)CO FBPFZTCFMRRESA-FSIIMWSLSA-N 0.000 claims description 4
- FBPFZTCFMRRESA-JGWLITMVSA-N D-glucitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-JGWLITMVSA-N 0.000 claims description 4
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 claims description 4
- RVGRUAULSDPKGF-UHFFFAOYSA-N Poloxamer Chemical compound C1CO1.CC1CO1 RVGRUAULSDPKGF-UHFFFAOYSA-N 0.000 claims description 4
- 125000000487 histidyl group Chemical group [H]N([H])C(C(=O)O*)C([H])([H])C1=C([H])N([H])C([H])=N1 0.000 claims description 4
- 238000010255 intramuscular injection Methods 0.000 claims description 4
- 239000007927 intramuscular injection Substances 0.000 claims description 4
- 229960000502 poloxamer Drugs 0.000 claims description 4
- 239000011780 sodium chloride Substances 0.000 claims description 4
- 235000002639 sodium chloride Nutrition 0.000 claims description 4
- 239000000600 sorbitol Substances 0.000 claims description 4
- 235000010356 sorbitol Nutrition 0.000 claims description 4
- 206010006187 Breast cancer Diseases 0.000 claims description 3
- 208000026310 Breast neoplasm Diseases 0.000 claims description 3
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims description 3
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 claims description 3
- 229930195725 Mannitol Natural products 0.000 claims description 3
- 206010061902 Pancreatic neoplasm Diseases 0.000 claims description 3
- 239000001110 calcium chloride Substances 0.000 claims description 3
- 229910001628 calcium chloride Inorganic materials 0.000 claims description 3
- 208000005017 glioblastoma Diseases 0.000 claims description 3
- 208000015486 malignant pancreatic neoplasm Diseases 0.000 claims description 3
- 239000000594 mannitol Substances 0.000 claims description 3
- 235000010355 mannitol Nutrition 0.000 claims description 3
- 201000002528 pancreatic cancer Diseases 0.000 claims description 3
- 208000008443 pancreatic carcinoma Diseases 0.000 claims description 3
- 229920000136 polysorbate Polymers 0.000 claims description 3
- 229950008882 polysorbate Drugs 0.000 claims description 3
- 206010005003 Bladder cancer Diseases 0.000 claims description 2
- ZGTMUACCHSMWAC-UHFFFAOYSA-L EDTA disodium salt (anhydrous) Chemical compound [Na+].[Na+].OC(=O)CN(CC([O-])=O)CCN(CC(O)=O)CC([O-])=O ZGTMUACCHSMWAC-UHFFFAOYSA-L 0.000 claims description 2
- WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 claims description 2
- 239000004471 Glycine Substances 0.000 claims description 2
- ONIBWKKTOPOVIA-BYPYZUCNSA-N L-Proline Chemical compound OC(=O)[C@@H]1CCCN1 ONIBWKKTOPOVIA-BYPYZUCNSA-N 0.000 claims description 2
- CKLJMWTZIZZHCS-REOHCLBHSA-N L-aspartic acid Chemical compound OC(=O)[C@@H](N)CC(O)=O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 claims description 2
- WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 claims description 2
- COLNVLDHVKWLRT-QMMMGPOBSA-N L-phenylalanine Chemical compound OC(=O)[C@@H](N)CC1=CC=CC=C1 COLNVLDHVKWLRT-QMMMGPOBSA-N 0.000 claims description 2
- 206010033128 Ovarian cancer Diseases 0.000 claims description 2
- 206010061535 Ovarian neoplasm Diseases 0.000 claims description 2
- ONIBWKKTOPOVIA-UHFFFAOYSA-N Proline Natural products OC(=O)C1CCCN1 ONIBWKKTOPOVIA-UHFFFAOYSA-N 0.000 claims description 2
- 208000006265 Renal cell carcinoma Diseases 0.000 claims description 2
- 206010039491 Sarcoma Diseases 0.000 claims description 2
- 208000007097 Urinary Bladder Neoplasms Diseases 0.000 claims description 2
- 235000003704 aspartic acid Nutrition 0.000 claims description 2
- OQFSQFPPLPISGP-UHFFFAOYSA-N beta-carboxyaspartic acid Natural products OC(=O)C(N)C(C(O)=O)C(O)=O OQFSQFPPLPISGP-UHFFFAOYSA-N 0.000 claims description 2
- 235000013922 glutamic acid Nutrition 0.000 claims description 2
- 239000004220 glutamic acid Substances 0.000 claims description 2
- 206010073071 hepatocellular carcinoma Diseases 0.000 claims description 2
- 231100000844 hepatocellular carcinoma Toxicity 0.000 claims description 2
- COLNVLDHVKWLRT-UHFFFAOYSA-N phenylalanine Natural products OC(=O)C(N)CC1=CC=CC=C1 COLNVLDHVKWLRT-UHFFFAOYSA-N 0.000 claims description 2
- 201000005112 urinary bladder cancer Diseases 0.000 claims description 2
- 235000014304 histidine Nutrition 0.000 claims 4
- 235000011148 calcium chloride Nutrition 0.000 claims 1
- 239000001201 calcium disodium ethylene diamine tetra-acetate Substances 0.000 claims 1
- 235000011188 calcium disodium ethylene diamine tetraacetate Nutrition 0.000 claims 1
- 235000008729 phenylalanine Nutrition 0.000 claims 1
- 235000013930 proline Nutrition 0.000 claims 1
- 239000003814 drug Substances 0.000 abstract description 24
- 229940079593 drug Drugs 0.000 abstract description 12
- 108010089836 Proto-Oncogene Proteins c-met Proteins 0.000 description 220
- 102000008022 Proto-Oncogene Proteins c-met Human genes 0.000 description 219
- 102000001301 EGF receptor Human genes 0.000 description 212
- 210000004027 cell Anatomy 0.000 description 75
- 102000004169 proteins and genes Human genes 0.000 description 51
- 108090000623 proteins and genes Proteins 0.000 description 51
- 235000018102 proteins Nutrition 0.000 description 48
- 238000002474 experimental method Methods 0.000 description 26
- 239000000203 mixture Substances 0.000 description 24
- 102100021102 Hyaluronidase PH-20 Human genes 0.000 description 23
- 101150055528 SPAM1 gene Proteins 0.000 description 23
- 238000012360 testing method Methods 0.000 description 22
- 229940024606 amino acid Drugs 0.000 description 20
- 235000001014 amino acid Nutrition 0.000 description 20
- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 20
- 239000002953 phosphate buffered saline Substances 0.000 description 20
- 238000001542 size-exclusion chromatography Methods 0.000 description 20
- 238000009472 formulation Methods 0.000 description 17
- 102100035360 Cerebellar degeneration-related antigen 1 Human genes 0.000 description 15
- 238000001035 drying Methods 0.000 description 15
- RAXXELZNTBOGNW-UHFFFAOYSA-N imidazole Chemical group C1=CNC=N1 RAXXELZNTBOGNW-UHFFFAOYSA-N 0.000 description 15
- 239000000047 product Substances 0.000 description 15
- 239000001963 growth medium Substances 0.000 description 11
- 230000005764 inhibitory process Effects 0.000 description 11
- 230000026731 phosphorylation Effects 0.000 description 11
- 238000006366 phosphorylation reaction Methods 0.000 description 11
- 238000005406 washing Methods 0.000 description 11
- 102100030127 Obscurin Human genes 0.000 description 10
- 101710194880 Obscurin Proteins 0.000 description 10
- 230000000694 effects Effects 0.000 description 10
- 239000012634 fragment Substances 0.000 description 10
- 239000013641 positive control Substances 0.000 description 10
- 208000024891 symptom Diseases 0.000 description 10
- 210000004881 tumor cell Anatomy 0.000 description 10
- 108010002947 Connectin Proteins 0.000 description 9
- 102100026260 Titin Human genes 0.000 description 9
- 230000000903 blocking effect Effects 0.000 description 9
- 208000002154 non-small cell lung carcinoma Diseases 0.000 description 9
- 238000012216 screening Methods 0.000 description 9
- 239000000126 substance Substances 0.000 description 9
- 208000029729 tumor suppressor gene on chromosome 11 Diseases 0.000 description 9
- 101000898034 Homo sapiens Hepatocyte growth factor Proteins 0.000 description 8
- 241001465754 Metazoa Species 0.000 description 8
- 238000007710 freezing Methods 0.000 description 8
- 230000008014 freezing Effects 0.000 description 8
- 230000004071 biological effect Effects 0.000 description 7
- 238000013461 design Methods 0.000 description 7
- 238000001514 detection method Methods 0.000 description 7
- 238000010494 dissociation reaction Methods 0.000 description 7
- 230000005593 dissociations Effects 0.000 description 7
- 239000000843 powder Substances 0.000 description 7
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 6
- 102100021866 Hepatocyte growth factor Human genes 0.000 description 6
- 241000699666 Mus <mouse, genus> Species 0.000 description 6
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 6
- 230000001413 cellular effect Effects 0.000 description 6
- 239000003795 chemical substances by application Substances 0.000 description 6
- 238000010586 diagram Methods 0.000 description 6
- 235000003642 hunger Nutrition 0.000 description 6
- 238000000338 in vitro Methods 0.000 description 6
- 239000007788 liquid Substances 0.000 description 6
- 239000002609 medium Substances 0.000 description 6
- 239000000825 pharmaceutical preparation Substances 0.000 description 6
- 239000000523 sample Substances 0.000 description 6
- DAEPDZWVDSPTHF-UHFFFAOYSA-M sodium pyruvate Chemical compound [Na+].CC(=O)C([O-])=O DAEPDZWVDSPTHF-UHFFFAOYSA-M 0.000 description 6
- 230000037351 starvation Effects 0.000 description 6
- 229940124597 therapeutic agent Drugs 0.000 description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 6
- JKMHFZQWWAIEOD-UHFFFAOYSA-N 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid Chemical compound OCC[NH+]1CCN(CCS([O-])(=O)=O)CC1 JKMHFZQWWAIEOD-UHFFFAOYSA-N 0.000 description 5
- QTBSBXVTEAMEQO-UHFFFAOYSA-M Acetate Chemical compound CC([O-])=O QTBSBXVTEAMEQO-UHFFFAOYSA-M 0.000 description 5
- CTKXFMQHOOWWEB-UHFFFAOYSA-N Ethylene oxide/propylene oxide copolymer Chemical compound CCCOC(C)COCCO CTKXFMQHOOWWEB-UHFFFAOYSA-N 0.000 description 5
- 239000007995 HEPES buffer Substances 0.000 description 5
- 108010021625 Immunoglobulin Fragments Proteins 0.000 description 5
- 102000008394 Immunoglobulin Fragments Human genes 0.000 description 5
- 239000002671 adjuvant Substances 0.000 description 5
- 239000007853 buffer solution Substances 0.000 description 5
- 238000011161 development Methods 0.000 description 5
- 238000005259 measurement Methods 0.000 description 5
- 239000000178 monomer Substances 0.000 description 5
- 239000013642 negative control Substances 0.000 description 5
- 229920001993 poloxamer 188 Polymers 0.000 description 5
- 229940044519 poloxamer 188 Drugs 0.000 description 5
- 108090000765 processed proteins & peptides Proteins 0.000 description 5
- -1 referred to as P188) Polymers 0.000 description 5
- 238000007920 subcutaneous administration Methods 0.000 description 5
- 101000851181 Homo sapiens Epidermal growth factor receptor Proteins 0.000 description 4
- 239000012980 RPMI-1640 medium Substances 0.000 description 4
- 125000000539 amino acid group Chemical group 0.000 description 4
- 238000004458 analytical method Methods 0.000 description 4
- 230000010056 antibody-dependent cellular cytotoxicity Effects 0.000 description 4
- 230000008859 change Effects 0.000 description 4
- SILCDLWESNHZKB-UHFFFAOYSA-L disodium 4-hydroxy-4-oxobutanoate Chemical compound [Na+].[Na+].OC(=O)CCC([O-])=O.OC(=O)CCC([O-])=O SILCDLWESNHZKB-UHFFFAOYSA-L 0.000 description 4
- 238000011156 evaluation Methods 0.000 description 4
- 238000012495 forced degradation study Methods 0.000 description 4
- 102000045108 human EGFR Human genes 0.000 description 4
- 230000003204 osmotic effect Effects 0.000 description 4
- 238000012510 peptide mapping method Methods 0.000 description 4
- 230000035755 proliferation Effects 0.000 description 4
- 230000019491 signal transduction Effects 0.000 description 4
- 239000002904 solvent Substances 0.000 description 4
- KDYFGRWQOYBRFD-UHFFFAOYSA-N succinic acid Chemical compound OC(=O)CCC(O)=O KDYFGRWQOYBRFD-UHFFFAOYSA-N 0.000 description 4
- 150000008163 sugars Chemical class 0.000 description 4
- 239000006228 supernatant Substances 0.000 description 4
- 230000001225 therapeutic effect Effects 0.000 description 4
- 102000009024 Epidermal Growth Factor Human genes 0.000 description 3
- 108060003951 Immunoglobulin Proteins 0.000 description 3
- 102000018071 Immunoglobulin Fc Fragments Human genes 0.000 description 3
- 108010091135 Immunoglobulin Fc Fragments Proteins 0.000 description 3
- 241000124008 Mammalia Species 0.000 description 3
- PXHVJJICTQNCMI-UHFFFAOYSA-N Nickel Chemical compound [Ni] PXHVJJICTQNCMI-UHFFFAOYSA-N 0.000 description 3
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 3
- 238000005119 centrifugation Methods 0.000 description 3
- 238000003745 diagnosis Methods 0.000 description 3
- CBMPTFJVXNIWHP-UHFFFAOYSA-L disodium;hydrogen phosphate;2-hydroxypropane-1,2,3-tricarboxylic acid Chemical compound [Na+].[Na+].OP([O-])([O-])=O.OC(=O)CC(O)(C(O)=O)CC(O)=O CBMPTFJVXNIWHP-UHFFFAOYSA-L 0.000 description 3
- 239000003937 drug carrier Substances 0.000 description 3
- 230000036541 health Effects 0.000 description 3
- 102000057421 human MET Human genes 0.000 description 3
- 102000018358 immunoglobulin Human genes 0.000 description 3
- 239000012535 impurity Substances 0.000 description 3
- 238000001727 in vivo Methods 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 230000004048 modification Effects 0.000 description 3
- 238000012986 modification Methods 0.000 description 3
- 230000035772 mutation Effects 0.000 description 3
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- BHZOKUMUHVTPBX-UHFFFAOYSA-M sodium acetic acid acetate Chemical compound [Na+].CC(O)=O.CC([O-])=O BHZOKUMUHVTPBX-UHFFFAOYSA-M 0.000 description 3
- 229940054269 sodium pyruvate Drugs 0.000 description 3
- 229960002920 sorbitol Drugs 0.000 description 3
- 230000002269 spontaneous effect Effects 0.000 description 3
- 239000013595 supernatant sample Substances 0.000 description 3
- XPFJYKARVSSRHE-UHFFFAOYSA-K trisodium;2-hydroxypropane-1,2,3-tricarboxylate;2-hydroxypropane-1,2,3-tricarboxylic acid Chemical compound [Na+].[Na+].[Na+].OC(=O)CC(O)(C(O)=O)CC(O)=O.[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O XPFJYKARVSSRHE-UHFFFAOYSA-K 0.000 description 3
- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 description 2
- YRNWIFYIFSBPAU-UHFFFAOYSA-N 4-[4-(dimethylamino)phenyl]-n,n-dimethylaniline Chemical compound C1=CC(N(C)C)=CC=C1C1=CC=C(N(C)C)C=C1 YRNWIFYIFSBPAU-UHFFFAOYSA-N 0.000 description 2
- 208000010507 Adenocarcinoma of Lung Diseases 0.000 description 2
- 240000003291 Armoracia rusticana Species 0.000 description 2
- 238000012492 Biacore method Methods 0.000 description 2
- 238000002965 ELISA Methods 0.000 description 2
- 229920002449 FKM Polymers 0.000 description 2
- 241000282412 Homo Species 0.000 description 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- 108010054477 Immunoglobulin Fab Fragments Proteins 0.000 description 2
- 102000001706 Immunoglobulin Fab Fragments Human genes 0.000 description 2
- LRQKBLKVPFOOQJ-YFKPBYRVSA-N L-norleucine Chemical compound CCCC[C@H]([NH3+])C([O-])=O LRQKBLKVPFOOQJ-YFKPBYRVSA-N 0.000 description 2
- 239000012515 MabSelect SuRe Substances 0.000 description 2
- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 description 2
- 241000699670 Mus sp. Species 0.000 description 2
- 229910019142 PO4 Inorganic materials 0.000 description 2
- 229920001213 Polysorbate 20 Polymers 0.000 description 2
- 102000001708 Protein Isoforms Human genes 0.000 description 2
- 108010029485 Protein Isoforms Proteins 0.000 description 2
- 206010041067 Small cell lung cancer Diseases 0.000 description 2
- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 2
- 230000003213 activating effect Effects 0.000 description 2
- 230000004913 activation Effects 0.000 description 2
- 239000004480 active ingredient Substances 0.000 description 2
- 230000002776 aggregation Effects 0.000 description 2
- 238000004220 aggregation Methods 0.000 description 2
- 230000003321 amplification Effects 0.000 description 2
- 238000003556 assay Methods 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- GUBGYTABKSRVRQ-QUYVBRFLSA-N beta-maltose Chemical compound OC[C@H]1O[C@H](O[C@H]2[C@H](O)[C@@H](O)[C@H](O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@@H]1O GUBGYTABKSRVRQ-QUYVBRFLSA-N 0.000 description 2
- 230000037396 body weight Effects 0.000 description 2
- 238000004364 calculation method Methods 0.000 description 2
- 201000011510 cancer Diseases 0.000 description 2
- MXZACTZQSGYANA-UHFFFAOYSA-N chembl545463 Chemical compound Cl.C1=CC(OC)=CC=C1C(N=C1)=CN2C1=NC(C)=C2O MXZACTZQSGYANA-UHFFFAOYSA-N 0.000 description 2
- 238000004587 chromatography analysis Methods 0.000 description 2
- 208000029742 colonic neoplasm Diseases 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 229940039227 diagnostic agent Drugs 0.000 description 2
- 239000000032 diagnostic agent Substances 0.000 description 2
- 238000010790 dilution Methods 0.000 description 2
- 239000012895 dilution Substances 0.000 description 2
- 238000006471 dimerization reaction Methods 0.000 description 2
- 238000004090 dissolution Methods 0.000 description 2
- 238000002296 dynamic light scattering Methods 0.000 description 2
- 238000001962 electrophoresis Methods 0.000 description 2
- 238000010828 elution Methods 0.000 description 2
- 239000000839 emulsion Substances 0.000 description 2
- 239000012530 fluid Substances 0.000 description 2
- 230000033581 fucosylation Effects 0.000 description 2
- 238000005227 gel permeation chromatography Methods 0.000 description 2
- 230000014509 gene expression Effects 0.000 description 2
- YMAWOPBAYDPSLA-UHFFFAOYSA-N glycylglycine Chemical compound [NH3+]CC(=O)NCC([O-])=O YMAWOPBAYDPSLA-UHFFFAOYSA-N 0.000 description 2
- 238000004128 high performance liquid chromatography Methods 0.000 description 2
- 102000057308 human HGF Human genes 0.000 description 2
- 238000001802 infusion Methods 0.000 description 2
- 230000002401 inhibitory effect Effects 0.000 description 2
- 230000003834 intracellular effect Effects 0.000 description 2
- 238000004255 ion exchange chromatography Methods 0.000 description 2
- 239000012669 liquid formulation Substances 0.000 description 2
- 201000005249 lung adenocarcinoma Diseases 0.000 description 2
- 238000012792 lyophilization process Methods 0.000 description 2
- CYAJEMFRSQGFIG-ISWIILBPSA-N microcystin-LA Natural products CO[C@@H](Cc1ccccc1)[C@@H](C)C=C(C)C=C[C@H](NC(=O)CNC(=O)[C@@H](C)[C@@H](NC(=O)[C@H](CC(C)C)NC(=O)[C@@H](C)NC(=O)C(=C)N(C)C(=O)CC[C@@H](C)C(=O)O)C(=O)O)[C@H](C)C(=O)N CYAJEMFRSQGFIG-ISWIILBPSA-N 0.000 description 2
- 230000007935 neutral effect Effects 0.000 description 2
- 238000003199 nucleic acid amplification method Methods 0.000 description 2
- 239000010452 phosphate Substances 0.000 description 2
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 2
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 description 2
- 229920001184 polypeptide Polymers 0.000 description 2
- 229940068977 polysorbate 20 Drugs 0.000 description 2
- 239000002243 precursor Substances 0.000 description 2
- 102000004196 processed proteins & peptides Human genes 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 238000001742 protein purification Methods 0.000 description 2
- 102000005962 receptors Human genes 0.000 description 2
- 108020003175 receptors Proteins 0.000 description 2
- 230000010076 replication Effects 0.000 description 2
- 239000004017 serum-free culture medium Substances 0.000 description 2
- 208000000587 small cell lung carcinoma Diseases 0.000 description 2
- 235000019333 sodium laurylsulphate Nutrition 0.000 description 2
- 241000894007 species Species 0.000 description 2
- 239000012089 stop solution Substances 0.000 description 2
- 239000001384 succinic acid Substances 0.000 description 2
- 210000001519 tissue Anatomy 0.000 description 2
- 238000013518 transcription Methods 0.000 description 2
- 230000035897 transcription Effects 0.000 description 2
- 238000013519 translation Methods 0.000 description 2
- LEAHFJQFYSDGGP-UHFFFAOYSA-K trisodium;dihydrogen phosphate;hydrogen phosphate Chemical compound [Na+].[Na+].[Na+].OP(O)([O-])=O.OP([O-])([O-])=O LEAHFJQFYSDGGP-UHFFFAOYSA-K 0.000 description 2
- 230000004614 tumor growth Effects 0.000 description 2
- QZNNVYOVQUKYSC-JEDNCBNOSA-N (2s)-2-amino-3-(1h-imidazol-5-yl)propanoic acid;hydron;chloride Chemical compound Cl.OC(=O)[C@@H](N)CC1=CN=CN1 QZNNVYOVQUKYSC-JEDNCBNOSA-N 0.000 description 1
- UKAUYVFTDYCKQA-UHFFFAOYSA-N -2-Amino-4-hydroxybutanoic acid Natural products OC(=O)C(N)CCO UKAUYVFTDYCKQA-UHFFFAOYSA-N 0.000 description 1
- BMYCCWYAFNPAQC-UHFFFAOYSA-N 2-[dodecyl(methyl)azaniumyl]acetate Chemical compound CCCCCCCCCCCCN(C)CC(O)=O BMYCCWYAFNPAQC-UHFFFAOYSA-N 0.000 description 1
- SNQVCAOGQHOSEN-UHFFFAOYSA-N 2-[methyl(octadecyl)amino]acetic acid Chemical compound CCCCCCCCCCCCCCCCCCN(C)CC(O)=O SNQVCAOGQHOSEN-UHFFFAOYSA-N 0.000 description 1
- QFJVDSDGRBUNKZ-UHFFFAOYSA-N 2-[methyl(tetradecyl)azaniumyl]acetate Chemical compound CCCCCCCCCCCCCCN(C)CC(O)=O QFJVDSDGRBUNKZ-UHFFFAOYSA-N 0.000 description 1
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 1
- DIROHOMJLWMERM-UHFFFAOYSA-N 3-[dimethyl(octadecyl)azaniumyl]propane-1-sulfonate Chemical compound CCCCCCCCCCCCCCCCCC[N+](C)(C)CCCS([O-])(=O)=O DIROHOMJLWMERM-UHFFFAOYSA-N 0.000 description 1
- UZOVYGYOLBIAJR-UHFFFAOYSA-N 4-isocyanato-4'-methyldiphenylmethane Chemical compound C1=CC(C)=CC=C1CC1=CC=C(N=C=O)C=C1 UZOVYGYOLBIAJR-UHFFFAOYSA-N 0.000 description 1
- PVXPPJIGRGXGCY-TZLCEDOOSA-N 6-O-alpha-D-glucopyranosyl-D-fructofuranose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1OC[C@@H]1[C@@H](O)[C@H](O)C(O)(CO)O1 PVXPPJIGRGXGCY-TZLCEDOOSA-N 0.000 description 1
- GUBGYTABKSRVRQ-XLOQQCSPSA-N Alpha-Lactose Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)O[C@H](O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-XLOQQCSPSA-N 0.000 description 1
- BMCZJPYYEFQOCO-UHFFFAOYSA-H C(CC(O)(C(=O)[O-])CC(=O)[O-])(=O)[O-].[Mg+2].C(CC(O)(C(=O)O)CC(=O)O)(=O)O.C(CC(O)(C(=O)[O-])CC(=O)[O-])(=O)[O-].[Mg+2].[Mg+2] Chemical compound C(CC(O)(C(=O)[O-])CC(=O)[O-])(=O)[O-].[Mg+2].C(CC(O)(C(=O)O)CC(=O)O)(=O)O.C(CC(O)(C(=O)[O-])CC(=O)[O-])(=O)[O-].[Mg+2].[Mg+2] BMCZJPYYEFQOCO-UHFFFAOYSA-H 0.000 description 1
- 101100476210 Caenorhabditis elegans rnt-1 gene Proteins 0.000 description 1
- 241000283707 Capra Species 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 208000005623 Carcinogenesis Diseases 0.000 description 1
- KRKNYBCHXYNGOX-UHFFFAOYSA-K Citrate Chemical compound [O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O KRKNYBCHXYNGOX-UHFFFAOYSA-K 0.000 description 1
- 108010047041 Complementarity Determining Regions Proteins 0.000 description 1
- HEBKCHPVOIAQTA-QWWZWVQMSA-N D-arabinitol Chemical compound OC[C@@H](O)C(O)[C@H](O)CO HEBKCHPVOIAQTA-QWWZWVQMSA-N 0.000 description 1
- RGHNJXZEOKUKBD-SQOUGZDYSA-M D-gluconate Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C([O-])=O RGHNJXZEOKUKBD-SQOUGZDYSA-M 0.000 description 1
- QWIZNVHXZXRPDR-UHFFFAOYSA-N D-melezitose Natural products O1C(CO)C(O)C(O)C(O)C1OC1C(O)C(CO)OC1(CO)OC1OC(CO)C(O)C(O)C1O QWIZNVHXZXRPDR-UHFFFAOYSA-N 0.000 description 1
- 230000004544 DNA amplification Effects 0.000 description 1
- 101000760085 Daucus carota 21 kDa protein Proteins 0.000 description 1
- 229920002307 Dextran Polymers 0.000 description 1
- FEWJPZIEWOKRBE-JCYAYHJZSA-N Dextrotartaric acid Chemical compound OC(=O)[C@H](O)[C@@H](O)C(O)=O FEWJPZIEWOKRBE-JCYAYHJZSA-N 0.000 description 1
- 101150039808 Egfr gene Proteins 0.000 description 1
- 239000004386 Erythritol Substances 0.000 description 1
- UNXHWFMMPAWVPI-UHFFFAOYSA-N Erythritol Natural products OCC(O)C(O)CO UNXHWFMMPAWVPI-UHFFFAOYSA-N 0.000 description 1
- IAYPIBMASNFSPL-UHFFFAOYSA-N Ethylene oxide Chemical compound C1CO1 IAYPIBMASNFSPL-UHFFFAOYSA-N 0.000 description 1
- 229910052693 Europium Inorganic materials 0.000 description 1
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 1
- 238000005033 Fourier transform infrared spectroscopy Methods 0.000 description 1
- 229930091371 Fructose Natural products 0.000 description 1
- 239000005715 Fructose Substances 0.000 description 1
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 description 1
- VZCYOOQTPOCHFL-OWOJBTEDSA-N Fumaric acid Chemical compound OC(=O)\C=C\C(O)=O VZCYOOQTPOCHFL-OWOJBTEDSA-N 0.000 description 1
- 230000005526 G1 to G0 transition Effects 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 108010008488 Glycylglycine Proteins 0.000 description 1
- 102000027430 HGF receptors Human genes 0.000 description 1
- 108091008603 HGF receptors Proteins 0.000 description 1
- 102000003745 Hepatocyte Growth Factor Human genes 0.000 description 1
- 108090000100 Hepatocyte Growth Factor Proteins 0.000 description 1
- 101001121408 Homo sapiens L-amino-acid oxidase Proteins 0.000 description 1
- 101000917858 Homo sapiens Low affinity immunoglobulin gamma Fc region receptor III-A Proteins 0.000 description 1
- PMMYEEVYMWASQN-DMTCNVIQSA-N Hydroxyproline Chemical compound O[C@H]1CN[C@H](C(O)=O)C1 PMMYEEVYMWASQN-DMTCNVIQSA-N 0.000 description 1
- 102100026120 IgG receptor FcRn large subunit p51 Human genes 0.000 description 1
- 101710177940 IgG receptor FcRn large subunit p51 Proteins 0.000 description 1
- 238000001265 Jonckheere trend test Methods 0.000 description 1
- 238000012313 Kruskal-Wallis test Methods 0.000 description 1
- 102100026388 L-amino-acid oxidase Human genes 0.000 description 1
- UKAUYVFTDYCKQA-VKHMYHEASA-N L-homoserine Chemical compound OC(=O)[C@@H](N)CCO UKAUYVFTDYCKQA-VKHMYHEASA-N 0.000 description 1
- QEFRNWWLZKMPFJ-ZXPFJRLXSA-N L-methionine (R)-S-oxide Chemical compound C[S@@](=O)CC[C@H]([NH3+])C([O-])=O QEFRNWWLZKMPFJ-ZXPFJRLXSA-N 0.000 description 1
- QEFRNWWLZKMPFJ-UHFFFAOYSA-N L-methionine sulphoxide Natural products CS(=O)CCC(N)C(O)=O QEFRNWWLZKMPFJ-UHFFFAOYSA-N 0.000 description 1
- JVTAAEKCZFNVCJ-UHFFFAOYSA-M Lactate Chemical compound CC(O)C([O-])=O JVTAAEKCZFNVCJ-UHFFFAOYSA-M 0.000 description 1
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 1
- 102100029193 Low affinity immunoglobulin gamma Fc region receptor III-A Human genes 0.000 description 1
- 208000007433 Lymphatic Metastasis Diseases 0.000 description 1
- NBGXQZRRLOGAJF-UHFFFAOYSA-N Maltulose Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)(CO)OCC1O NBGXQZRRLOGAJF-UHFFFAOYSA-N 0.000 description 1
- 206010027476 Metastases Diseases 0.000 description 1
- 102100031914 Obscurin-like protein 1 Human genes 0.000 description 1
- 101710130995 Obscurin-like protein 1 Proteins 0.000 description 1
- 241000283973 Oryctolagus cuniculus Species 0.000 description 1
- MUBZPKHOEPUJKR-UHFFFAOYSA-N Oxalic acid Chemical compound OC(=O)C(O)=O MUBZPKHOEPUJKR-UHFFFAOYSA-N 0.000 description 1
- 102000007079 Peptide Fragments Human genes 0.000 description 1
- 108010033276 Peptide Fragments Proteins 0.000 description 1
- GOOHAUXETOMSMM-UHFFFAOYSA-N Propylene oxide Chemical compound CC1CO1 GOOHAUXETOMSMM-UHFFFAOYSA-N 0.000 description 1
- MUPFEKGTMRGPLJ-RMMQSMQOSA-N Raffinose Natural products O(C[C@H]1[C@@H](O)[C@H](O)[C@@H](O)[C@@H](O[C@@]2(CO)[C@H](O)[C@@H](O)[C@@H](CO)O2)O1)[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 MUPFEKGTMRGPLJ-RMMQSMQOSA-N 0.000 description 1
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 description 1
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 description 1
- 208000015634 Rectal Neoplasms Diseases 0.000 description 1
- 101100012902 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) FIG2 gene Proteins 0.000 description 1
- 229920002684 Sepharose Polymers 0.000 description 1
- 108010003723 Single-Domain Antibodies Proteins 0.000 description 1
- UQZIYBXSHAGNOE-USOSMYMVSA-N Stachyose Natural products O(C[C@H]1[C@@H](O)[C@H](O)[C@H](O)[C@@H](O[C@@]2(CO)[C@H](O)[C@@H](O)[C@@H](CO)O2)O1)[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@H](CO[C@@H]2[C@@H](O)[C@@H](O)[C@@H](O)[C@H](CO)O2)O1 UQZIYBXSHAGNOE-USOSMYMVSA-N 0.000 description 1
- 238000000692 Student's t-test Methods 0.000 description 1
- QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 description 1
- MUPFEKGTMRGPLJ-UHFFFAOYSA-N UNPD196149 Natural products OC1C(O)C(CO)OC1(CO)OC1C(O)C(O)C(O)C(COC2C(C(O)C(O)C(CO)O2)O)O1 MUPFEKGTMRGPLJ-UHFFFAOYSA-N 0.000 description 1
- 238000001793 Wilcoxon signed-rank test Methods 0.000 description 1
- BHATUINFZWUDIX-UHFFFAOYSA-N Zwittergent 3-14 Chemical compound CCCCCCCCCCCCCC[N+](C)(C)CCCS([O-])(=O)=O BHATUINFZWUDIX-UHFFFAOYSA-N 0.000 description 1
- 230000002159 abnormal effect Effects 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 239000000443 aerosol Substances 0.000 description 1
- 238000001042 affinity chromatography Methods 0.000 description 1
- LHAOFBCHXGZGOR-NAVBLJQLSA-N alpha-D-Manp-(1->3)-alpha-D-Manp-(1->2)-alpha-D-Manp Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@H](O)[C@H]1O[C@@H]1[C@@H](O)[C@@H](O[C@@H]2[C@H]([C@@H](O)[C@H](O)[C@@H](CO)O2)O)[C@H](O)[C@@H](CO)O1 LHAOFBCHXGZGOR-NAVBLJQLSA-N 0.000 description 1
- 125000003277 amino group Chemical group 0.000 description 1
- 229940008421 amivantamab Drugs 0.000 description 1
- 230000033115 angiogenesis Effects 0.000 description 1
- 230000008485 antagonism Effects 0.000 description 1
- 238000011091 antibody purification Methods 0.000 description 1
- 230000000890 antigenic effect Effects 0.000 description 1
- 238000000149 argon plasma sintering Methods 0.000 description 1
- 238000003149 assay kit Methods 0.000 description 1
- 230000003305 autocrine Effects 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 239000013060 biological fluid Substances 0.000 description 1
- 229920001400 block copolymer Polymers 0.000 description 1
- 238000009530 blood pressure measurement Methods 0.000 description 1
- 210000004899 c-terminal region Anatomy 0.000 description 1
- VSGNNIFQASZAOI-UHFFFAOYSA-L calcium acetate Chemical compound [Ca+2].CC([O-])=O.CC([O-])=O VSGNNIFQASZAOI-UHFFFAOYSA-L 0.000 description 1
- QIIZPUIPFGEZFO-UHFFFAOYSA-L calcium;4-hydroxy-4-oxobutanoate Chemical compound [Ca+2].OC(=O)CCC([O-])=O.OC(=O)CCC([O-])=O QIIZPUIPFGEZFO-UHFFFAOYSA-L 0.000 description 1
- 230000036952 cancer formation Effects 0.000 description 1
- 238000005251 capillar electrophoresis Methods 0.000 description 1
- 238000013368 capillary electrophoresis sodium dodecyl sulfate analysis Methods 0.000 description 1
- 238000001818 capillary gel electrophoresis Methods 0.000 description 1
- 238000000533 capillary isoelectric focusing Methods 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- UHBYWPGGCSDKFX-UHFFFAOYSA-N carboxyglutamic acid Chemical compound OC(=O)C(N)CC(C(O)=O)C(O)=O UHBYWPGGCSDKFX-UHFFFAOYSA-N 0.000 description 1
- 231100000504 carcinogenesis Toxicity 0.000 description 1
- 239000000969 carrier Substances 0.000 description 1
- 230000004663 cell proliferation Effects 0.000 description 1
- 238000003570 cell viability assay Methods 0.000 description 1
- 150000005829 chemical entities Chemical class 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000000546 chi-square test Methods 0.000 description 1
- 229940001468 citrate Drugs 0.000 description 1
- 230000001447 compensatory effect Effects 0.000 description 1
- 230000000295 complement effect Effects 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 230000006240 deamidation Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 238000004925 denaturation Methods 0.000 description 1
- 230000036425 denaturation Effects 0.000 description 1
- 238000000502 dialysis Methods 0.000 description 1
- 239000003085 diluting agent Substances 0.000 description 1
- VNGHLDVVHPMNAG-UHFFFAOYSA-L dipotassium butanedioate butanedioic acid Chemical compound [K+].[K+].OC(=O)CCC(O)=O.[O-]C(=O)CCC([O-])=O VNGHLDVVHPMNAG-UHFFFAOYSA-L 0.000 description 1
- 238000000375 direct analysis in real time Methods 0.000 description 1
- 150000002016 disaccharides Chemical class 0.000 description 1
- PMMYEEVYMWASQN-UHFFFAOYSA-N dl-hydroxyproline Natural products OC1C[NH2+]C(C([O-])=O)C1 PMMYEEVYMWASQN-UHFFFAOYSA-N 0.000 description 1
- 238000007876 drug discovery Methods 0.000 description 1
- 238000012063 dual-affinity re-targeting Methods 0.000 description 1
- 230000008482 dysregulation Effects 0.000 description 1
- 229940121647 egfr inhibitor Drugs 0.000 description 1
- 229920001971 elastomer Polymers 0.000 description 1
- 230000005611 electricity Effects 0.000 description 1
- 108700021358 erbB-1 Genes Proteins 0.000 description 1
- UNXHWFMMPAWVPI-ZXZARUISSA-N erythritol Chemical compound OC[C@H](O)[C@H](O)CO UNXHWFMMPAWVPI-ZXZARUISSA-N 0.000 description 1
- 229940009714 erythritol Drugs 0.000 description 1
- 235000019414 erythritol Nutrition 0.000 description 1
- OGPBJKLSAFTDLK-UHFFFAOYSA-N europium atom Chemical compound [Eu] OGPBJKLSAFTDLK-UHFFFAOYSA-N 0.000 description 1
- 238000009093 first-line therapy Methods 0.000 description 1
- 238000000684 flow cytometry Methods 0.000 description 1
- 238000001506 fluorescence spectroscopy Methods 0.000 description 1
- 235000019256 formaldehyde Nutrition 0.000 description 1
- 229940050411 fumarate Drugs 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 229940050410 gluconate Drugs 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 229940043257 glycylglycine Drugs 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 238000000703 high-speed centrifugation Methods 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 150000002431 hydrogen Chemical group 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 229960002591 hydroxyproline Drugs 0.000 description 1
- 230000001900 immune effect Effects 0.000 description 1
- 210000000987 immune system Anatomy 0.000 description 1
- 238000003125 immunofluorescent labeling Methods 0.000 description 1
- 230000005847 immunogenicity Effects 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 238000001361 intraarterial administration Methods 0.000 description 1
- 238000007918 intramuscular administration Methods 0.000 description 1
- 238000007912 intraperitoneal administration Methods 0.000 description 1
- 238000001990 intravenous administration Methods 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 238000005342 ion exchange Methods 0.000 description 1
- 239000003456 ion exchange resin Substances 0.000 description 1
- 229920003303 ion-exchange polymer Polymers 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- FZWBNHMXJMCXLU-BLAUPYHCSA-N isomaltotriose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1OC[C@@H]1[C@@H](O)[C@H](O)[C@@H](O)[C@@H](OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C=O)O1 FZWBNHMXJMCXLU-BLAUPYHCSA-N 0.000 description 1
- 238000006317 isomerization reaction Methods 0.000 description 1
- 239000000832 lactitol Substances 0.000 description 1
- 235000010448 lactitol Nutrition 0.000 description 1
- VQHSOMBJVWLPSR-JVCRWLNRSA-N lactitol Chemical compound OC[C@H](O)[C@@H](O)[C@@H]([C@H](O)CO)O[C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O VQHSOMBJVWLPSR-JVCRWLNRSA-N 0.000 description 1
- 229960003451 lactitol Drugs 0.000 description 1
- 239000008101 lactose Substances 0.000 description 1
- JCQLYHFGKNRPGE-FCVZTGTOSA-N lactulose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 JCQLYHFGKNRPGE-FCVZTGTOSA-N 0.000 description 1
- 229960000511 lactulose Drugs 0.000 description 1
- PFCRQPBOOFTZGQ-UHFFFAOYSA-N lactulose keto form Natural products OCC(=O)C(O)C(C(O)CO)OC1OC(CO)C(O)C(O)C1O PFCRQPBOOFTZGQ-UHFFFAOYSA-N 0.000 description 1
- IZWSFJTYBVKZNK-UHFFFAOYSA-N lauryl sulfobetaine Chemical compound CCCCCCCCCCCC[N+](C)(C)CCCS([O-])(=O)=O IZWSFJTYBVKZNK-UHFFFAOYSA-N 0.000 description 1
- 238000004895 liquid chromatography mass spectrometry Methods 0.000 description 1
- 238000004020 luminiscence type Methods 0.000 description 1
- 239000012139 lysis buffer Substances 0.000 description 1
- UEGPKNKPLBYCNK-UHFFFAOYSA-L magnesium acetate Chemical compound [Mg+2].CC([O-])=O.CC([O-])=O UEGPKNKPLBYCNK-UHFFFAOYSA-L 0.000 description 1
- 239000000845 maltitol Substances 0.000 description 1
- 235000010449 maltitol Nutrition 0.000 description 1
- VQHSOMBJVWLPSR-WUJBLJFYSA-N maltitol Chemical compound OC[C@H](O)[C@@H](O)[C@@H]([C@H](O)CO)O[C@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O VQHSOMBJVWLPSR-WUJBLJFYSA-N 0.000 description 1
- 229940035436 maltitol Drugs 0.000 description 1
- JCQLYHFGKNRPGE-HFZVAGMNSA-N maltulose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 JCQLYHFGKNRPGE-HFZVAGMNSA-N 0.000 description 1
- FYGDTMLNYKFZSV-UHFFFAOYSA-N mannotriose Natural products OC1C(O)C(O)C(CO)OC1OC1C(CO)OC(OC2C(OC(O)C(O)C2O)CO)C(O)C1O FYGDTMLNYKFZSV-UHFFFAOYSA-N 0.000 description 1
- 238000004949 mass spectrometry Methods 0.000 description 1
- 238000000816 matrix-assisted laser desorption--ionisation Methods 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- QWIZNVHXZXRPDR-WSCXOGSTSA-N melezitose Chemical compound O([C@@]1(O[C@@H]([C@H]([C@@H]1O[C@@H]1[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O1)O)O)CO)CO)[C@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O QWIZNVHXZXRPDR-WSCXOGSTSA-N 0.000 description 1
- HEBKCHPVOIAQTA-UHFFFAOYSA-N meso ribitol Natural products OCC(O)C(O)C(O)CO HEBKCHPVOIAQTA-UHFFFAOYSA-N 0.000 description 1
- 230000009401 metastasis Effects 0.000 description 1
- 230000001394 metastastic effect Effects 0.000 description 1
- 206010061289 metastatic neoplasm Diseases 0.000 description 1
- LSDPWZHWYPCBBB-UHFFFAOYSA-O methylsulfide anion Chemical compound [SH2+]C LSDPWZHWYPCBBB-UHFFFAOYSA-O 0.000 description 1
- 108091005601 modified peptides Proteins 0.000 description 1
- 150000002772 monosaccharides Chemical class 0.000 description 1
- 229910052759 nickel Inorganic materials 0.000 description 1
- 239000002736 nonionic surfactant Substances 0.000 description 1
- HEGSGKPQLMEBJL-RKQHYHRCSA-N octyl beta-D-glucopyranoside Chemical compound CCCCCCCCO[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O HEGSGKPQLMEBJL-RKQHYHRCSA-N 0.000 description 1
- 229950000846 onartuzumab Drugs 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 238000013495 osmolality determination method Methods 0.000 description 1
- 230000002018 overexpression Effects 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 230000003076 paracrine Effects 0.000 description 1
- 238000007911 parenteral administration Methods 0.000 description 1
- 230000037361 pathway Effects 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- BZQFBWGGLXLEPQ-REOHCLBHSA-N phosphoserine Chemical compound OC(=O)[C@@H](N)COP(O)(O)=O BZQFBWGGLXLEPQ-REOHCLBHSA-N 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- FHUOTRMCFQTSOA-UHFFFAOYSA-M potassium;acetic acid;acetate Chemical compound [K+].CC(O)=O.CC([O-])=O FHUOTRMCFQTSOA-UHFFFAOYSA-M 0.000 description 1
- BUCIWTBCUUHRHZ-UHFFFAOYSA-K potassium;disodium;dihydrogen phosphate;hydrogen phosphate Chemical compound [Na+].[Na+].[K+].OP(O)([O-])=O.OP([O-])([O-])=O BUCIWTBCUUHRHZ-UHFFFAOYSA-K 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 230000003449 preventive effect Effects 0.000 description 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
- 230000000069 prophylactic effect Effects 0.000 description 1
- 238000000159 protein binding assay Methods 0.000 description 1
- 230000005180 public health Effects 0.000 description 1
- MUPFEKGTMRGPLJ-ZQSKZDJDSA-N raffinose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO[C@@H]2[C@@H]([C@@H](O)[C@@H](O)[C@@H](CO)O2)O)O1 MUPFEKGTMRGPLJ-ZQSKZDJDSA-N 0.000 description 1
- 102000027426 receptor tyrosine kinases Human genes 0.000 description 1
- 108091008598 receptor tyrosine kinases Proteins 0.000 description 1
- 206010038038 rectal cancer Diseases 0.000 description 1
- 201000001275 rectum cancer Diseases 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 238000012552 review Methods 0.000 description 1
- 102200048955 rs121434569 Human genes 0.000 description 1
- 229940043230 sarcosine Drugs 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 239000012679 serum free medium Substances 0.000 description 1
- 230000011664 signaling Effects 0.000 description 1
- 238000003998 size exclusion chromatography high performance liquid chromatography Methods 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 235000015424 sodium Nutrition 0.000 description 1
- 229940074404 sodium succinate Drugs 0.000 description 1
- ZDQYSKICYIVCPN-UHFFFAOYSA-L sodium succinate (anhydrous) Chemical compound [Na+].[Na+].[O-]C(=O)CCC([O-])=O ZDQYSKICYIVCPN-UHFFFAOYSA-L 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 230000000087 stabilizing effect Effects 0.000 description 1
- UQZIYBXSHAGNOE-XNSRJBNMSA-N stachyose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO[C@@H]2[C@@H]([C@@H](O)[C@@H](O)[C@@H](CO[C@@H]3[C@@H]([C@@H](O)[C@@H](O)[C@@H](CO)O3)O)O2)O)O1 UQZIYBXSHAGNOE-XNSRJBNMSA-N 0.000 description 1
- 238000000528 statistical test Methods 0.000 description 1
- KDYFGRWQOYBRFD-UHFFFAOYSA-L succinate(2-) Chemical compound [O-]C(=O)CCC([O-])=O KDYFGRWQOYBRFD-UHFFFAOYSA-L 0.000 description 1
- 150000005846 sugar alcohols Chemical class 0.000 description 1
- 229940117986 sulfobetaine Drugs 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 230000008685 targeting Effects 0.000 description 1
- 229940095064 tartrate Drugs 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- FGMPLJWBKKVCDB-UHFFFAOYSA-N trans-L-hydroxy-proline Natural products ON1CCCC1C(O)=O FGMPLJWBKKVCDB-UHFFFAOYSA-N 0.000 description 1
- VZCYOOQTPOCHFL-UHFFFAOYSA-N trans-butenedioic acid Natural products OC(=O)C=CC(O)=O VZCYOOQTPOCHFL-UHFFFAOYSA-N 0.000 description 1
- SYSWJPSVYLUKCH-UHFFFAOYSA-H tricalcium;2-hydroxypropane-1,2,3-tricarboxylate;2-hydroxypropane-1,2,3-tricarboxylic acid Chemical compound [Ca+2].[Ca+2].[Ca+2].OC(=O)CC(O)(C(O)=O)CC(O)=O.[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O.[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O SYSWJPSVYLUKCH-UHFFFAOYSA-H 0.000 description 1
- SPRBJFWIUVWXBT-UHFFFAOYSA-K tripotassium 2-hydroxypropane-1,2,3-tricarboxylate 2-hydroxypropane-1,2,3-tricarboxylic acid Chemical compound [K+].[K+].[K+].OC(=O)CC(O)(C(O)=O)CC(O)=O.[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O SPRBJFWIUVWXBT-UHFFFAOYSA-K 0.000 description 1
- 150000004043 trisaccharides Chemical class 0.000 description 1
- GPRLSGONYQIRFK-MNYXATJNSA-N triton Chemical compound [3H+] GPRLSGONYQIRFK-MNYXATJNSA-N 0.000 description 1
- 229940121358 tyrosine kinase inhibitor Drugs 0.000 description 1
- 239000005483 tyrosine kinase inhibitor Substances 0.000 description 1
- 238000000108 ultra-filtration Methods 0.000 description 1
- 238000009777 vacuum freeze-drying Methods 0.000 description 1
- 230000000007 visual effect Effects 0.000 description 1
- 238000011179 visual inspection Methods 0.000 description 1
- 239000011534 wash buffer Substances 0.000 description 1
- 239000003643 water by type Substances 0.000 description 1
- 239000000080 wetting agent Substances 0.000 description 1
- 229950008250 zalutumumab Drugs 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/395—Antibodies; Immunoglobulins; Immune serum, e.g. antilymphocytic serum
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
- C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
Definitions
- the present disclosure belongs to the field of pharmaceutical preparations, and specifically relates to a pharmaceutical composition comprising a bispecific antibody that specifically binds to HGFR and EGFR, and its use as a drug.
- Epidermal growth factor receptor (EGFR) and hepatocyte growth factor receptor (HGFR) are two receptor tyrosine kinases that are highly expressed on a variety of tumor cells, such as non-small cell lung cancer (NSCLC), colorectal cancer (CRC) and head and neck cancer (HNC).
- NSCLC non-small cell lung cancer
- CRC colorectal cancer
- HNC head and neck cancer
- the EGFR signaling pathway plays an important role in tumor biology by regulating cell proliferation, angiogenesis, metastasis and survival of cancer cells. Dysregulation of this pathway can lead to tumorigenesis. HGFR is also associated with the occurrence of many tumors due to abnormal signal activation caused by overexpression, activating mutations, autocrine or paracrine signal transduction or gene amplification of HGFR. Investigations into cancer efficacy have found that there is an important connection between the EGFR and HGFR signaling pathways.
- NSCLC accounts for 83% of all lung cancers, and EGFR activating mutations are the most common type (10-15% in whites and 50% in Asians).
- EGFR tyrosine kinase inhibitors (TKIs) have always been the first-line therapy for NSCLC. Although the initial response rate is high (70-80%), resistance usually occurs within a year. There are two main mechanisms of resistance. One is the emergence of another EGFR mutation, such as EGFR T790M; the other is the compensatory activation of the HGFR signaling pathway. These factors all affect the treatment effect of tumors.
- the present disclosure provides a pharmaceutical composition
- a pharmaceutical composition comprising a bispecific antibody that specifically binds to HGFR and EGFR, the composition having therapeutic activity.
- the composition also has advantages such as good stability.
- the present disclosure provides a pharmaceutical composition
- a pharmaceutical composition comprising a bispecific antibody that specifically binds to HGFR and EGFR and a buffer, wherein:
- the bispecific antibody that specifically binds to HGFR and EGFR comprises at least one antigen binding module 1 that specifically binds to HGFR and at least one antigen binding module 2 that specifically binds to EGFR;
- the buffer is histidine buffer, acetate buffer, citrate buffer, succinate buffer or phosphate buffer.
- the present disclosure provides a pharmaceutical composition
- a pharmaceutical composition comprising a bispecific antibody that specifically binds to HGFR and EGFR and a buffer, wherein:
- the bispecific antibody that specifically binds to HGFR and EGFR comprises at least one antigen binding module 1 that specifically binds to HGFR and at least one antigen binding module 2 that specifically binds to EGFR;
- the antigen binding module 1 comprises a heavy chain variable region M-VH and a light chain variable region M-VL, wherein the M-VH comprises M-HCDR1, M-HCDR2 and M-HCDR3, wherein the M-VL comprises M-LCDR1, M-LCDR2 and M-LCDR3, and
- the antigen binding module 2 comprises a heavy chain variable region E-VH and a light chain variable region E-VL, wherein the E-VH comprises E-HCDR1, E-HCDR2 and E-HCDR3, and the E-VL comprises E-LCDR1, E-LCDR2 and E-LCDR3,
- E-HCDR1, E-HCDR2, E-HCDR3, E-LCDR1, E-LCDR2 and E-LCDR3 are defined according to the Kabat numbering rules, the E-HCDR1 is shown as SEQ ID NO: 6, the E-HCDR2 is shown as SEQ ID NO: 7, the E-HCDR3 is shown as SEQ ID NO: 8, the E-LCDR1 is shown as SEQ ID NO: 9, the E-LCDR2 is shown as SEQ ID NO: 10, and the E-LCDR3 is shown as SEQ ID NO: 11; and
- M-HCDR1, M-HCDR2, M-HCDR3, M-LCDR1, M-LCDR2 and M-LCDR3 are defined according to the Kabat numbering convention, wherein:
- the M-HCDR1 is shown as SEQ ID NO: 30
- the M-HCDR2 is shown as SEQ ID NO: 31
- the M-HCDR3 is shown as SEQ ID NO: 32
- the M-LCDR1 is shown as SEQ ID NO: 27
- the M-LCDR2 is shown as SEQ ID NO: 33
- the M-LCDR3 is shown as SEQ ID NO: 29, or
- the M-HCDR1 comprises SEQ ID NO: 18, the M-HCDR2 comprises SEQ ID NO: 19, the M-HCDR3 comprises SEQ ID NO: 20, the M-LCDR1 comprises SEQ ID NO: 21, the M-LCDR2 comprises SEQ ID NO: 22, and the M-LCDR3 comprises SEQ ID NO: 23, or
- the M-HCDR1 is shown as SEQ ID NO: 24, the M-HCDR2 is shown as SEQ ID NO: 25, the M-HCDR3 is shown as SEQ ID NO: 26, the M-LCDR1 is shown as SEQ ID NO: 27, the M-LCDR2 is shown as SEQ ID NO: 28, and the M-LCDR3 is shown as SEQ ID NO: 29;
- the buffer is histidine buffer, acetate buffer, citrate buffer, succinate buffer or phosphate buffer.
- the buffer is a histidine buffer.
- the buffer is histidine-histidine hydrochloride buffer or histidine-histidine acetate buffer.
- the buffer is histidine-histidine hydrochloride buffer.
- the pharmaceutical composition as described in any of the above, wherein the pH of the pharmaceutical composition is 4.5 to 6.5. In some embodiments, the pharmaceutical composition as described in any of the above, wherein the pH of the pharmaceutical composition is 4.8 to 5.2. In some embodiments, the pH of the pharmaceutical composition is 5.0 to 6.0.
- the pH of the pharmaceutical composition is 5.0 to 6.5. In some embodiments, the pH of the pharmaceutical composition is 5.5 to 6.5. In some embodiments, the pH of the pharmaceutical composition is In some embodiments, the pH of the pharmaceutical composition is between 5.5 and 6.0. In some embodiments, the pH of the pharmaceutical composition is between 6.0 and 6.5. In some embodiments, the pharmaceutical composition as described in any of the above, wherein the pH of the pharmaceutical composition is between 5.8 and 6.2. In some embodiments, the pH of the pharmaceutical composition is about 5.8. In some embodiments, the pH of the pharmaceutical composition is about 6.0. In some embodiments, the pH of the pharmaceutical composition is about 6.2.
- the pharmaceutical composition as described in any of the above, wherein the pH of the pharmaceutical composition is 4.5 to 6.0. In some embodiments, the pharmaceutical composition as described in any of the above, wherein the pH of the pharmaceutical composition is 4.5 to 5.5. In some embodiments, the pharmaceutical composition as described in any of the above, wherein the pH of the pharmaceutical composition is 4.8 to 5.2. In some embodiments, the pH of the pharmaceutical composition is about 4.8. In some embodiments, the pH of the pharmaceutical composition is about 5.0. In some embodiments, the pH of the pharmaceutical composition is about 5.2.
- the point value includes a margin of error.
- This margin of error is due to factors such as laboratory environment, personnel operation, instrumentation, methodology, and measurement error.
- pH when the measured value is about 6.0, it should be understood that it includes a margin of error.
- about 6.0 means 6.0 ⁇ 0.2 (i.e., pH is 5.8 to 6.2).
- the pH of the pharmaceutical composition is 4.5, 4.6, 4.7, 4.8, 4.9, 5.0, 5.1, 5.2, 5.3, 5.4, 5.5, 5.6, 5.7, 5.8, 5.9, 6.0, 6.1, 6.2, 6.3, 6.4 or 6.5, or any range between these point values.
- the pH of the pharmaceutical composition is 4.8.
- the pH of the pharmaceutical composition is 5.0.
- the pH of the pharmaceutical composition is 5.2.
- the pH of the pharmaceutical composition is 5.8.
- the pH of the pharmaceutical composition is 6.0.
- the pH of the pharmaceutical composition is 6.2.
- the pH of the pharmaceutical composition obtained by replacing the buffer is almost the same as the buffer pH.
- the pH drift of the pharmaceutical preparation is generally very small (e.g., within the range of ⁇ 0.3).
- the pH drift of the pharmaceutical preparation is within the range of ⁇ 0.3.
- the pH drift of the pharmaceutical preparation is within the range of ⁇ 0.2.
- the pH drift of the pharmaceutical preparation is within the range of ⁇ 0.1.
- the pharmaceutical composition as described in any one of the above, wherein the concentration of the bispecific antibody that specifically binds HGFR and EGFR is 1 mg/mL to 250 mg/mL. In some embodiments, the concentration of the bispecific antibody that specifically binds HGFR and EGFR is 1 mg/mL to 200 mg/mL. In some embodiments, the concentration of the bispecific antibody that specifically binds HGFR and EGFR is 50 mg/mL to 200 mg/mL. In some embodiments, the concentration of the bispecific antibody that specifically binds HGFR and EGFR is 80 mg/mL to 180 mg/mL.
- the concentration of the bispecific antibody that specifically binds to HGFR and EGFR is 1 mg/mL to 150 mg/mL. In some embodiments, the concentration of the bispecific antibody that specifically binds to HGFR and EGFR is 1 mg/mL to 120 mg/mL. In some embodiments, the concentration of the bispecific antibody that specifically binds to HGFR and EGFR is 1 mg/mL to 100 mg/mL. In some embodiments, In some embodiments, the concentration of the bispecific antibody that specifically binds HGFR and EGFR is 50 mg/mL to 150 mg/mL.
- the concentration of the bispecific antibody that specifically binds HGFR and EGFR is 80 mg/mL to 120 mg/mL. In some embodiments, the concentration of the bispecific antibody that specifically binds HGFR and EGFR is 90 mg/mL to 110 mg/mL. In some embodiments, the concentration of the bispecific antibody that specifically binds HGFR and EGFR is about 100 mg/mL.
- the concentration of the bispecific antibody that specifically binds HGFR and EGFR is 80 mg/mL to 200 mg/mL. In some embodiments, the concentration of the bispecific antibody that specifically binds HGFR and EGFR is 90 mg/mL to 200 mg/mL. In some embodiments, the concentration of the bispecific antibody that specifically binds HGFR and EGFR is 100 mg/mL to 200 mg/mL. In some embodiments, the concentration of the bispecific antibody that specifically binds HGFR and EGFR is 120 mg/mL to 200 mg/mL. In some embodiments, the concentration of the bispecific antibody that specifically binds HGFR and EGFR is 120 mg/mL to 180 mg/mL.
- the concentration of the bispecific antibody that specifically binds HGFR and EGFR is 135 mg/mL to 165 mg/mL. In some embodiments, the concentration of the bispecific antibody that specifically binds HGFR and EGFR is about 150 mg/mL.
- the concentration of the bispecific antibody that specifically binds HGFR and EGFR is 50 mg/mL to 100 mg/mL. In some embodiments, the concentration of the bispecific antibody that specifically binds HGFR and EGFR is 50 mg/mL to 75 mg/mL. In some embodiments, the concentration of the bispecific antibody that specifically binds HGFR and EGFR is 48 mg/mL to 72 mg/mL. In some embodiments, the concentration of the bispecific antibody that specifically binds HGFR and EGFR is 54 mg/mL to 66 mg/mL. In some embodiments, the concentration of the bispecific antibody that specifically binds HGFR and EGFR is about 60 mg/mL. In some embodiments, the concentration of the bispecific antibody that specifically binds HGFR and EGFR is about 70 mg/mL.
- the concentration of the bispecific antibody that specifically binds HGFR and EGFR is 50 mg/mL to 250 mg/mL. In some embodiments, the concentration of the bispecific antibody that specifically binds HGFR and EGFR is 100 mg/mL to 250 mg/mL. In some embodiments, the concentration of the bispecific antibody that specifically binds HGFR and EGFR is 160 mg/mL to 240 mg/mL. In some embodiments, the concentration of the bispecific antibody that specifically binds HGFR and EGFR is 180 mg/mL to 220 mg/mL. In some embodiments, the concentration of the bispecific antibody that specifically binds HGFR and EGFR is about 200 mg/mL.
- the concentration of the bispecific antibody that specifically binds to HGFR and EGFR is 1 mg/mL, 5 mg/mL, 10 mg/mL, 20 mg/mL, 30 mg/mL, 40 mg/mL, 45 mg/mL, 48 mg/mL, 50 mg/mL, 54 mg/mL, 55 mg/mL, 60 mg/mL, 65 mg/mL, 66 mg/mL, 70 mg/mL, 72 mg/mL, 75 mg/mL, 80 mg/mL L, 85 mg/mL, 90 mg/mL, 95 mg/mL, 100 mg/mL, 105 mg/mL, 110 mg/mL, 120 mg/mL, 130 mg/mL, 135 mg/mL, 140 mg/mL, 150 mg/mL, 160 mg/mL, 165 mg/mL, 180 mg/mL, 200 mg/mL, 220 mg/mL, 240 mg/mL or 250 mg/mL, or any range between these point
- the specific The concentration of the bispecific antibody that binds HGFR and EGFR is 60 mg/mL. In some embodiments, the concentration of the bispecific antibody that specifically binds HGFR and EGFR is 70 mg/mL. In some embodiments, the concentration of the bispecific antibody that specifically binds HGFR and EGFR is 100 mg/mL. In some embodiments, the concentration of the bispecific antibody that specifically binds HGFR and EGFR is 150 mg/mL. In some embodiments, the concentration of the bispecific antibody that specifically binds HGFR and EGFR is 200 mg/mL.
- the surfactant is a nonionic surfactant.
- the surfactant is selected from poloxamer (e.g., poloxamer 188, referred to as P188), polysorbate (e.g., polysorbate 20, polysorbate 80), polyhydroxyalkylene, Triton, sodium dodecyl sulfate, sodium lauryl sulfate, sodium octyl glucoside, lauryl-sulfobetaine, myristyl-sulfobetaine, linoleyl-sulfobetaine, stearyl-sulfobetaine, lauryl-sarcosine, myristyl-sarcosine, linoleyl-sarcosine, stearyl-sarcosine, linoleyl-sweet In some embodiments,
- the surfactant concentration is 0.3 mg/mL to 0.6 mg/mL. In some embodiments, the surfactant concentration is 0.4 mg/mL to 0.6 mg/mL. In some embodiments, the surfactant concentration is 0.6 mg/mL to 0.8 mg/mL. In some embodiments, the surfactant concentration is 0.2 mg/mL to 0.4 mg/mL. In some embodiments, the surfactant concentration is 0.2 mg/mL to 0.3 mg/mL. In some embodiments, the surfactant concentration is 0.24 mg/mL to 0.36 mg/mL. In some embodiments, the surfactant concentration is about 0.24 mg/mL. In some embodiments, the surfactant concentration is about 0.3 mg/mL.
- the surfactant concentration is 0.01 mg/mL, 0.05 mg/mL, 0.1 mg/mL, In some embodiments, the surfactant concentration is 0.15mg/mL, 0.2mg/mL, 0.24mg/mL, 0.3mg/mL, 0.36mg/mL, 0.4mg/mL, 0.5mg/mL, 0.6mg/mL, 0.7mg/mL, 0.8mg/mL, 0.9mg/mL or 1.0mg/mL, or any range between these point values. In some embodiments, the surfactant concentration is 0.6mg/mL. In some embodiments, the surfactant concentration is 0.24mg/mL. In some embodiments, the surfactant concentration is 0.3mg/mL.
- the surfactant is 0.6 mg/mL polysorbate 80. In some embodiments, the surfactant concentration is 0.24 mg/mL polysorbate 80. In some embodiments, the surfactant concentration is 0.3 mg/mL polysorbate 80.
- the pharmaceutical composition as described in any one of the above comprises sugar.
- the sugar is selected from the conventional composition ( CH2O ) n and its derivatives, including monosaccharides, disaccharides, trisaccharides, polysaccharides, sugar alcohols, reducing sugars, non-reducing sugars, etc.
- the sugar can be selected from sucrose, trehalose, glucose, lactose, fructose, maltose, dextran, glycerol, erythritol, glycerol, arabitol, sylitol, sorbitol, mannitol, melezitose, raffinose, mannotriose, stachyose, maltose, lactulose, maltulose, sorbitol, maltitol, lactitol, iso-maltulose, etc.
- the sugar is sucrose, trehalose, mannitol, or sorbitol. In some embodiments, the sugar is sucrose or trehalose. In some embodiments, the sugar is sucrose.
- the concentration of the sugar is 10 mg/mL to 100 mg/mL. In some embodiments, the concentration of the sugar is 20 mg/mL to 90 mg/mL. In some embodiments, the concentration of the sugar is 20 mg/mL to 80 mg/mL. In some embodiments, the concentration of the sugar is 30 mg/mL to 80 mg/mL. In some embodiments, the concentration of the sugar is 50 mg/mL to 80 mg/mL. In some embodiments, the concentration of the sugar is 60 mg/mL to 90 mg/mL. In some embodiments, the concentration of the sugar is 60 mg/mL to 80 mg/mL. In some embodiments, the concentration of the sugar is 67.5 mg/mL to 82.5 mg/mL. In some embodiments, the concentration of the sugar is 70 mg/mL to 80 mg/mL. In some embodiments, the concentration of the sugar is about 75 mg/mL.
- the concentration of the sugar is 56 mg/mL to 84 mg/mL. In some embodiments, the concentration of the sugar is 63 mg/mL to 77 mg/mL. In some embodiments, the concentration of the sugar is about 70 mg/mL.
- the concentration of the sugar is 20 mg/mL to 50 mg/mL. In some embodiments, the concentration of the sugar is 20 mg/mL to 40 mg/mL. In some embodiments, the concentration of the sugar is 20 mg/mL to 35 mg/mL. In some embodiments, the concentration of the sugar is 22.4 mg/mL to 33.6 mg/mL. In some embodiments, the concentration of the sugar is 25.2 mg/mL to 30.8 mg/mL. In some embodiments, the concentration of the sugar is about 28 mg/mL.
- the concentration of the sugar is 30 mg/mL to 50 mg/mL. In some embodiments, the concentration of the sugar is 30 mg/mL to 40 mg/mL. In some embodiments, the concentration of the sugar is about 37.5 mg/mL.
- the concentration of the sugar includes, but is not limited to, 10 mg/mL, 20 mg/mL, In some embodiments, the sugar concentration is 22.4mg/mL, 25.2mg/mL, 28mg/mL, 30mg/mL, 30.8mg/mL, 33.6mg/mL, 35mg/mL, 37.5mg/mL, 40mg/mL, 45mg/mL, 50mg/mL, 56mg/mL, 55mg/mL, 60mg/mL, 63mg/mL, 65mg/mL, 67.5mg/mL, 70mg/mL, 75mg/mL, 77mg/mL, 80mg/mL, 82.5mg/mL, 84mg/mL, 85mg/mL, 90mg/mL, 95mg/mL, 100mg/mL, and any range between these point values.
- the sugar concentration is 28mg/mL. In some embodiments, the sugar concentration is 37.5mg/mL. In some embodiments, the sugar concentration is 70mg/mL. In some embodiments, the sugar concentration is 75mg/mL.
- the sugar is 70 mg/mL to 80 mg/mL of sucrose; preferably, the sugar is 75 mg/mL of sucrose.
- the sugar is 63 mg/mL to 77 mg/mL of sucrose; preferably, the sugar is 70 mg/mL of sucrose.
- the sugar is 25.2 mg/mL to 30.8 mg/mL of sucrose; preferably, the sugar is 28 mg/mL of sucrose.
- the concentration of the buffer is 5mM to 100mM. In some embodiments, the concentration of the buffer is 5mM to 50mM. In some embodiments, the concentration of the buffer is 10mM to 50mM. In some embodiments, the concentration of the buffer is 5mM to 30mM. In some embodiments, the concentration of the buffer is 10mM to 30mM. In some embodiments, the concentration of the buffer is 15mM to 25mM. In some embodiments, the concentration of the buffer is 18mM to 22mM. In some embodiments, the concentration of the buffer is 10mM to 20mM. In some embodiments, the concentration of the buffer is 10mM or 20mM. In some embodiments, the concentration of the buffer is about 20mM.
- the concentration of the buffer is 20 mM to 30 mM. In some embodiments, the concentration of the buffer is 22.5 mM to 27.5 mM. In some embodiments, the concentration of the buffer is about 25 mM.
- the concentration of the buffer is 5mM to 15mM. In some embodiments, the concentration of the buffer is 8mM to 12mM. In some embodiments, the concentration of the buffer is 9mM to 11mM. In some embodiments, the concentration of the buffer is about 10mM.
- the concentration of the buffer is 5mM, 8mM, 9mM, 10mM, 11mM, 12mM, 15mM, 18mM, 20mM, 22mM, 22.5mM, 25mM, 27.5mM, 30mM, 40mM, 50mM, 60mM, 70mM, 80mM, 90mM or 100mM, and any range between these point values.
- the concentration of the buffer is 10mM.
- the concentration of the buffer is 20mM. In some embodiments, the concentration of the buffer is 25mM.
- the buffer is 10 mM or 20 mM histidine-histidine hydrochloride buffer. In some embodiments, the buffer is 10 mM or 20 mM histidine-histidine acetate buffer. In some embodiments, the buffer is 25 mM histidine-histidine hydrochloride buffer.
- the excipient is methionine, arginine hydrochloride, glycine, proline, histidine, phenylalanine, glutamic acid, aspartic acid, sodium chloride, calcium chloride or disodium edetate.
- the excipient is sodium chloride or calcium chloride.
- the excipient is methionine or arginine hydrochloride.
- the excipient is arginine hydrochloride.
- the excipient is methionine.
- the concentration of the excipient is 1 mM to 10 mM. In some embodiments, the concentration of the excipient is 3 mM to 5 mM. In some embodiments, the concentration of the excipient is 3.6 mM to 4.4 mM. In some embodiments, the concentration of the excipient is about 4 mM.
- the concentration of the excipient is 1 mM to 240 mM. In some embodiments, the concentration of the excipient is 80 mM to 240 mM. In some embodiments, the concentration of the excipient is 160 mM to 240 mM. In some embodiments, the concentration of the excipient is 180 mM to 220 mM. In some embodiments, the concentration of the excipient is about 200 mM.
- the concentration of the excipient is 80 mM to 120 mM. In some embodiments, the concentration of the excipient is 90 mM to 110 mM. In some embodiments, the concentration of the excipient is about 100 mM.
- the concentration of the adjuvant is 1mM, 3mM, 3.6mM, 4.4mM, 5mM, 8mM, 9mM, 10mM, 11mM, 12mM, 20mM, 25mM, 30mM, 50mM, 75mM, 80mM, 90mM, 100mM, 110mM, 120mM, 150mM, 160mM, 180mM, 200mM, 240mM, 250mM, 280mM or 300mM, and any range between these point values.
- the concentration of the adjuvant is 4mM.
- the concentration of the adjuvant is 10mM.
- the pharmaceutical composition as described in any one of the above, wherein the concentration of the adjuvant is 100mM.
- the enzyme is hyaluronidase (PH20).
- the pharmaceutical composition as described in any one of the above, wherein the concentration of the enzyme is 10 In some embodiments, the pharmaceutical composition as described in any one of the above, wherein the concentration of the enzyme is 150U/mL to 2400U/mL. In some embodiments, the pharmaceutical composition as described in any one of the above, wherein the concentration of the enzyme is 150U/mL to 2200U/mL. In some embodiments, the pharmaceutical composition as described in any one of the above, wherein the concentration of the enzyme is 600U/mL to 2200U/mL. In some embodiments, the pharmaceutical composition as described in any one of the above, wherein the concentration of the enzyme is 800U/mL to 2000U/mL.
- the pharmaceutical composition as described in any one of the above, wherein the concentration of the enzyme is 700U/mL to 2400U/mL. In some embodiments, the pharmaceutical composition as described in any one of the above, wherein the concentration of the enzyme is 800U/mL to 2400U/mL. In some embodiments, the pharmaceutical composition as described in any one of the above, wherein the concentration of the enzyme is 1600U/mL to 2400U/mL. In some embodiments, the pharmaceutical composition as described in any one of the above, wherein the concentration of the enzyme is 1800U/mL to 2200U/mL. In some embodiments, the pharmaceutical composition as described in any one of the above, wherein the concentration of the enzyme is about 2000U/mL.
- the pharmaceutical composition as described in any one of the above, wherein the concentration of the enzyme is 150U/mL to 2000U/mL. In some embodiments, the pharmaceutical composition as described in any one of the above, wherein the concentration of the enzyme is 500U/mL to 1100U/mL. In some embodiments, the pharmaceutical composition as described in any one of the above, wherein the concentration of the enzyme is 600U/mL to 1000U/mL. In some embodiments, the pharmaceutical composition as described in any one of the above, wherein the concentration of the enzyme is 640U/mL to 960U/mL. In some embodiments, the pharmaceutical composition as described in any one of the above, wherein the concentration of the enzyme is 700U/mL to 900U/mL.
- the pharmaceutical composition as described in any one of the above, wherein the concentration of the enzyme is 720U/mL to 880U/mL. In some embodiments, the pharmaceutical composition as described in any one of the above, wherein the concentration of the enzyme is about 800U/mL.
- the concentration of the enzyme is 10U/mL, 50U/mL, 100U/mL, 150U/mL, 300U/mL, 500U/mL, 600U/mL, 640U/mL, 700U/mL, 720U/mL, 800U/mL, 880U/mL, 900U/mL, 960U/mL, 1000U/mL, 1100U/mL, 1200U/mL, 1500U/mL, 1600U/mL, 1800U/mL, 2000U/mL, 2200U/mL, 2400U/mL, 2500U/mL, 2800U/mL or 3000U/mL, and any range between these point values.
- the pharmaceutical composition as described in any one of the above, wherein the concentration of the enzyme is 800U/mL. In some embodiments, the pharmaceutical composition as described in any of the above, wherein the concentration of the enzyme is 1000 U/mL. In some embodiments, the pharmaceutical composition as described in any of the above, wherein the concentration of the enzyme is 2000 U/mL.
- the pharmaceutical composition as described in any one of the above, wherein the enzyme is 1000U/mL or 2000U/mL of PH20. In some embodiments, the pharmaceutical composition as described in any one of the above, wherein the enzyme is 800U/mL of PH20.
- the pharmaceutical composition as described in any of the above items wherein the bispecific antibody that specifically binds to HGFR and EGFR,
- the antigen binding module 1 comprises a heavy chain variable region M-VH and a light chain variable region M-VL, wherein the M-VH comprising M-HCDR1, M-HCDR2 and M-HCDR3, wherein the M-VL comprises M-LCDR1, M-LCDR2 and M-LCDR3, wherein:
- E-HCDR1, E-HCDR2, E-HCDR3, E-LCDR1, E-LCDR2 and E-LCDR3 are defined according to the Kabat numbering rules, the E-HCDR1 is shown as SEQ ID NO: 6, the E-HCDR2 is shown as SEQ ID NO: 7, the E-HCDR3 is shown as SEQ ID NO: 8, the E-LCDR1 is shown as SEQ ID NO: 9, the E-LCDR2 is shown as SEQ ID NO: 10, and the E-LCDR3 is shown as SEQ ID NO: 11; and
- M-HCDR1, M-HCDR2, M-HCDR3, M-LCDR1, M-LCDR2 and M-LCDR3 are defined according to the Kabat numbering convention, wherein:
- the M-HCDR1 is shown as SEQ ID NO: 30
- the M-HCDR2 is shown as SEQ ID NO: 31
- the M-HCDR3 is shown as SEQ ID NO: 32
- the M-LCDR1 is shown as SEQ ID NO: 27
- the M-LCDR2 is shown as SEQ ID NO: 33
- the M-LCDR3 is shown as SEQ ID NO: 29, or
- the M-HCDR1 comprises SEQ ID NO: 18, the M-HCDR2 comprises SEQ ID NO: 19, the M-HCDR3 comprises SEQ ID NO: 20, the M-LCDR1 comprises SEQ ID NO: 21, the M-LCDR2 comprises SEQ ID NO: 22, and the M-LCDR3 comprises SEQ ID NO: 23, or
- the M-HCDR1 is shown as SEQ ID NO: 24, the M-HCDR2 is shown as SEQ ID NO: 25, the M-HCDR3 is shown as SEQ ID NO: 26, the M-LCDR1 is shown as SEQ ID NO: 27, the M-LCDR2 is shown as SEQ ID NO: 28, and the M-LCDR3 is shown as SEQ ID NO: 29;
- the bispecific antibody that specifically binds to HGFR and EGFR comprises two antigen binding modules 1 that specifically bind to HGFR and one antigen binding module 2 that specifically binds to EGFR,
- One of the antigen binding modules 1, its M-VH has: M-HCDR1 as shown in SEQ ID NO: 30, M-HCDR2 as shown in SEQ ID NO: 31 and M-HCDR3 as shown in SEQ ID NO: 32, and its M-VL has: M-LCDR1 as shown in SEQ ID NO: 27, M-LCDR2 as shown in SEQ ID NO: 33 and M-LCDR3 as shown in SEQ ID NO: 29;
- Another antigen binding module 1 whose M-VH has: M-HCDR1 as shown in SEQ ID NO: 18, M-HCDR2 as shown in SEQ ID NO: 19 and M-HCDR3 as shown in SEQ ID NO: 20, and whose M-VL has: M-LCDR1 as shown in SEQ ID NO: 21, M-LCDR2 as shown in SEQ ID NO: 22 and M-LCDR3 as shown in SEQ ID NO: 23; and
- the antigen binding module 2 whose E-VH has: E-HCDR1 as shown in SEQ ID NO: 6, E-HCDR2 as shown in SEQ ID NO: 7 and E-HCDR3 as shown in SEQ ID NO: 8, and its E-VL has: E-LCDR1 as shown in SEQ ID NO: 9, E-LCDR2 as shown in SEQ ID NO: 10 and E-LCDR3 as shown in SEQ ID NO: 11; or
- the bispecific antibody that specifically binds to HGFR and EGFR comprises an antigen binding module 1 that specifically binds to HGFR and an antigen binding module 2 that specifically binds to EGFR,
- the antigen binding module 1 has: M-HCDR1 as shown in SEQ ID NO: 18, M-HCDR2 as shown in SEQ ID NO: 19 and M-HCDR3 as shown in SEQ ID NO: 20, and its M-VL has: M-LCDR1 as shown in SEQ ID NO: 21, M-LCDR2 as shown in SEQ ID NO: 22 and M-LCDR3 as shown in SEQ ID NO: 23; and
- the antigen binding module 2 has: E-HCDR1 as shown in SEQ ID NO: 6, E-HCDR2 as shown in SEQ ID NO: 7 and E-HCDR3 as shown in SEQ ID NO: 8, and its E-VL has: E-LCDR1 as shown in SEQ ID NO: 9, E-LCDR2 as shown in SEQ ID NO: 10 and E-LCDR3 as shown in SEQ ID NO: 11.
- the M-VH comprises an amino acid sequence having at least 90% sequence identity to SEQ ID NO: 16
- the M-VL comprises an amino acid sequence having at least 90% sequence identity to SEQ ID NO: 17;
- the M-VH comprises an amino acid sequence having at least 90% sequence identity to SEQ ID NO: 12
- the M-VL comprises an amino acid sequence having at least 90% sequence identity to SEQ ID NO: 13;
- the M-VH comprises an amino acid sequence having at least 90% sequence identity with SEQ ID NO: 14
- the M-VL comprises an amino acid sequence having at least 90% sequence identity with SEQ ID NO: 15;
- the E-VH comprises an amino acid sequence having at least 90% sequence identity to SEQ ID NO: 3
- the E-VL comprises an amino acid sequence having at least 90% sequence identity to SEQ ID NO: 5 or SEQ ID NO: 4;
- the bispecific antibody that specifically binds to HGFR and EGFR comprises two antigen binding modules 1 that specifically bind to HGFR and one antigen binding module 2 that specifically binds to EGFR,
- the M-VH of one of the antigen binding modules 1 is shown as SEQ ID NO: 16, and the M-VL is shown as SEQ ID NO: 17;
- the M-VH of another antigen binding module 1 is shown as SEQ ID NO: 12, and the M-VL is shown as SEQ ID NO: 13; and
- the E-VH of the antigen binding module 2 is shown as SEQ ID NO: 3, and the E-VL is shown as SEQ ID NO: 5; or
- the bispecific antibody that specifically binds to HGFR and EGFR comprises an antigen binding module 1 that specifically binds to HGFR and an antigen binding module 2 that specifically binds to EGFR,
- the M-VH of the antigen binding module 1 is shown in SEQ ID NO: 12, and the M-VL is shown in SEQ ID NO:
- the antigen binding module 2 is shown in SEQ ID NO: 13
- the E-VH of the antigen binding module 2 is shown in SEQ ID NO: 3
- the E-VL is shown in SEQ ID NO: 5.
- composition as described in any of the above items, wherein:
- the bispecific antibody that specifically binds to HGFR and EGFR has a first chain as shown in SEQ ID NO: 34, a second chain comprising SEQ ID NO: 35, a third chain comprising SEQ ID NO: 36 and a fourth chain comprising SEQ ID NO: 37; or
- SEQ ID NO: 38 It has a first chain shown as SEQ ID NO: 38, a second chain shown as SEQ ID NO: 39, a third chain shown as SEQ ID NO: 40 and a fourth chain shown as SEQ ID NO: 41.
- the bispecific antibody that specifically binds to HGFR and EGFR has a Format 1 or Format 2 structure
- the structural schematic diagram of Format 1 is shown in Figure 1
- the structural schematic diagram of Format 2 is shown in Figure 2.
- the pharmaceutical composition as described in any of the above items comprises the following components:
- the pharmaceutical composition as described in any of the above items comprises the following components:
- the pharmaceutical composition as described in any of the above items comprises the following components:
- the pharmaceutical composition as described in any of the above items comprises the following components:
- the pharmaceutical composition as described in any of the above items comprises the following components:
- the pharmaceutical composition as described in any of the above items comprises the following components:
- the pharmaceutical composition as described in any of the above items comprises the following components:
- the pharmaceutical composition as described in any of the above items comprises the following components:
- the pharmaceutical composition as described in any of the above items comprises the following components:
- the pharmaceutical composition as described in any of the above items comprises the following components:
- the pharmaceutical composition as described in any of the above items comprises the following components:
- the pharmaceutical composition as described in any of the above items comprises the following components:
- the pharmaceutical composition as described in any of the above items comprises the following components:
- the pharmaceutical composition as described in any of the above items comprises the following components:
- the pharmaceutical composition as described in any of the above items comprises the following components:
- the pharmaceutical composition as described in any of the above items comprises the following components:
- the pharmaceutical composition as described in any of the above items comprises the following components:
- the pharmaceutical composition as described in any of the above items comprises the following components:
- the pharmaceutical composition as described in any of the above items comprises the following components:
- the pharmaceutical composition as described in any of the above items comprises the following components:
- the pharmaceutical composition as described in any of the above items comprises the following components:
- the pharmaceutical composition as described in any of the above items comprises the following components:
- the pharmaceutical composition as described in any of the above items comprises the following components:
- the pharmaceutical composition as described in any of the above items comprises the following components:
- the pharmaceutical composition as described in any of the above items comprises the following components:
- the pharmaceutical composition as described in any of the above items comprises the following components:
- the pharmaceutical composition as described in any of the above items comprises the following components:
- the pharmaceutical composition as described in any of the above items comprises the following components:
- the pharmaceutical composition as described in any of the above items comprises the following components:
- the pharmaceutical composition as described in any of the above items comprises the following components:
- the pharmaceutical composition as described in any of the above items comprises the following components:
- the pharmaceutical composition as described in any of the above items comprises the following components:
- the pharmaceutical composition as described in any of the above items comprises the following components:
- the pharmaceutical composition as described in any of the above items comprises the following components:
- the pharmaceutical composition as described in any of the above items comprises the following components:
- the pharmaceutical composition as described in any of the above items comprises the following components:
- the pharmaceutical composition as described in any of the above items comprises the following components:
- the pharmaceutical composition as described in any of the above items comprises the following components:
- the pharmaceutical composition as described in any of the above items comprises the following components:
- the pharmaceutical composition as described in any of the above items comprises the following components:
- the pharmaceutical composition as described in any of the above items comprises the following components:
- the pharmaceutical composition as described in any of the above items comprises the following components:
- the pharmaceutical composition as described in any of the above items comprises the following components:
- the pharmaceutical composition as described in any of the above items comprises the following components:
- the pharmaceutical composition as described in any of the above items comprises the following components:
- the pharmaceutical composition as described in any of the above items comprises the following components:
- the pharmaceutical composition as described in any of the above items comprises the following components:
- the pharmaceutical composition is a liquid preparation.
- the solvent of the liquid preparation is water.
- the present disclosure also provides a lyophilized preparation, characterized in that the lyophilized preparation can form a pharmaceutical composition as described in any of the above items after being reconstituted.
- the present disclosure also provides a lyophilized preparation, which is a lyophilized preparation of the pharmaceutical composition as described in any one of the above items.
- the present disclosure also provides a method for preparing a lyophilized preparation, comprising the step of freeze-drying the pharmaceutical composition as described in any one of the above items.
- the freeze-drying as described in any one of the above items sequentially comprises the steps of pre-freezing, primary drying and secondary drying.
- the present disclosure also provides a lyophilized preparation, which is obtained by freeze-drying the pharmaceutical composition as described in any one of the above items.
- the present disclosure also provides a reconstituted solution, characterized in that the reconstituted solution is prepared by reconstituted the lyophilized preparation as described in any one of the above items.
- the present disclosure also provides a reconstituted solution, which is a reconstituted preparation of the lyophilized preparation as described in any one of the above items.
- the components and contents of the reconstituted solution as described in any of the above items are the same as the components and contents of the aforementioned pharmaceutical composition.
- the reconstituted solution as described in any of the above items comprises the following components:
- the reconstituted solution as described in any of the above items comprises the following components:
- the reconstituted solution as described in any of the above items comprises the following components:
- the reconstituted solution as described in any of the above items comprises the following components:
- the reconstituted solution as described in any of the above items comprises the following components:
- the reconstituted solution as described in any of the above items comprises the following components:
- the reconstituted solution as described in any of the above items comprises the following components:
- the reconstituted solution as described in any of the above items comprises the following components:
- the reconstituted solution as described in any of the above items comprises the following components:
- the reconstituted solution as described in any of the above items comprises the following components:
- the reconstituted solution as described in any of the above items comprises the following components:
- the reconstituted solution as described in any of the above items comprises the following components:
- the reconstituted solution as described in any of the above items comprises the following components:
- the reconstituted solution as described in any of the above items comprises the following components:
- the reconstituted solution as described in any of the above items comprises the following components:
- the reconstituted solution as described in any of the above items comprises the following components:
- the reconstituted solution as described in any of the above items comprises the following components:
- the reconstituted solution as described in any of the above items comprises the following components:
- the reconstituted solution as described in any of the above items comprises the following components:
- the reconstituted solution as described in any of the above items comprises the following components:
- the reconstituted solution as described in any of the above items comprises the following components:
- the reconstituted solution as described in any of the above items comprises the following components:
- the reconstituted solution as described in any of the above items comprises the following components:
- the reconstituted solution as described in any of the above items comprises the following components:
- the reconstituted solution as described in any of the above items comprises the following components:
- the reconstituted solution as described in any of the above items comprises the following components:
- the reconstituted solution as described in any of the above items comprises the following components:
- the reconstituted solution as described in any of the above items comprises the following components:
- the reconstituted solution as described in any of the above items comprises the following components:
- the reconstituted solution as described in any of the above items comprises the following components:
- the reconstituted solution as described in any of the above items comprises the following components:
- the reconstituted solution as described in any of the above items comprises the following components:
- the reconstituted solution as described in any of the above items comprises the following components:
- the reconstituted solution as described in any of the above items comprises the following components:
- the reconstituted solution as described in any of the above items comprises the following components:
- the reconstituted solution as described in any of the above items comprises the following components:
- the reconstituted solution as described in any of the above items comprises the following components:
- the reconstituted solution as described in any of the above items comprises the following components:
- the reconstituted solution as described in any of the above items comprises the following components:
- the reconstituted solution as described in any of the above items comprises the following components:
- the reconstituted solution as described in any of the above items comprises the following components:
- the reconstituted solution as described in any of the above items comprises the following components:
- the reconstituted solution as described in any of the above items comprises the following components:
- the reconstituted solution as described in any of the above items comprises the following components:
- the reconstituted solution as described in any of the above items comprises the following components:
- the reconstituted solution as described in any of the above items comprises the following components:
- the reconstituted solution as described in any of the above items comprises the following components:
- the pharmaceutical composition or reconstituted solution as described in any of the above items is an intravenous injection preparation, a subcutaneous injection preparation, an intraperitoneal injection preparation, or an intramuscular injection preparation. In some embodiments, the pharmaceutical composition or reconstituted solution as described in any of the above items is an intravenous injection preparation. In some embodiments, the pharmaceutical composition or reconstituted solution as described in any of the above items is a subcutaneous injection preparation.
- the pharmaceutical composition or reconstituted solution as described in any of the above items is suitable for intravenous injection, subcutaneous injection, intraperitoneal injection or intramuscular injection. In some embodiments, the pharmaceutical composition or reconstituted solution as described in any of the above items is suitable for intravenous injection. In some embodiments, the pharmaceutical composition or reconstituted solution as described in any of the above items is suitable for subcutaneous injection.
- the pharmaceutical composition, reconstituted solution or lyophilized preparation as described in any of the above items is used to prepare a drug for intravenous injection, subcutaneous injection, intraperitoneal injection or intramuscular injection. In some embodiments, the pharmaceutical composition, reconstituted solution or lyophilized preparation as described in any of the above items is used to prepare a drug for intravenous injection. In some embodiments, the pharmaceutical composition, reconstituted solution or lyophilized preparation as described in any of the above items is used to prepare a drug for subcutaneous injection.
- the present disclosure also provides a medicine kit, which comprises at least one container, each container independently comprising the pharmaceutical composition as described in any one of the above items, the lyophilized preparation as described in any one of the above items, or the reconstituted solution as described in any one of the above items.
- the present disclosure also provides a method for diagnosing, treating, and alleviating a subject's condition, comprising administering to the subject an effective amount of the pharmaceutical composition as described in any of the above, the lyophilized preparation as described in any of the above, the reconstituted solution as described in any of the above, or the medicine kit as described in any of the above.
- the present disclosure also provides a method for treating or preventing a disease, the method comprising administering to a subject a therapeutically effective amount of the pharmaceutical composition as described in any one of the above, the lyophilized preparation as described in any one of the above, the reconstituted solution as described in any one of the above, or the medicine kit as described in any one of the above.
- the present disclosure also provides a pharmaceutical composition as described in any of the above, a lyophilized preparation as described in any of the above, a reconstituted solution as described in any of the above, or a medicine kit as described in any of the above, for use in treating or preventing a disease.
- the present disclosure also provides use of a pharmaceutical composition as described in any of the preceding items, a lyophilized preparation as described in any of the preceding items, a reconstituted solution as described in any of the preceding items, or a drug kit as described in any of the preceding items in the preparation of a medicament for preventing or treating a disease or condition.
- the disease described in any of the above items is a tumor.
- the tumor is selected from lung cancer (including non-small cell lung cancer and small cell lung cancer), breast cancer, pancreatic cancer, colorectal cancer (including colon cancer and rectal cancer), sarcoma, renal cell carcinoma, hepatocellular carcinoma, gastric cancer, ovarian cancer, bladder cancer, head and neck cancer and glioblastoma.
- the tumor is selected from lung cancer, breast cancer, pancreatic cancer, colon cancer, head and neck cancer, gastric cancer and glioblastoma.
- the lung cancer is non-small cell lung cancer.
- the lung cancer is metastatic non-small cell lung cancer.
- the lung cancer is small cell lung cancer.
- the lung cancer is human lung adenocarcinoma.
- the lung cancer is gastric cancer.
- the tumor is an EGFR and/or HGFR-related tumor.
- Figure 1 Schematic diagram of the structure of Format1.
- FIG. 1 Schematic diagram of the structure of Format2.
- Figure 3 The results of the antibody-EGFR CHO-S cell binding activity experiment, where the vertical axis of the figure is the mean fluorescence intensity (MFI for short, the same below).
- Figure 4 Antibody binding activity experimental results with HGFR CHO-S cells.
- Figure 5 Antibody binding activity assay results for H1975-HGF cells.
- Figure 6 Antibody binding activity assay results for MKN-45 cells.
- FIG. 7 The results of the antibody inhibition experiment on cell EGFR phosphorylation.
- FIG8 The results of the antibody inhibition experiment on cell HGFR phosphorylation.
- FIG. 9 The results of the antibody inhibition experiment on cell AKT phosphorylation.
- FIG. 10 Experimental results of antibody reduction of cell surface HGFR.
- FIG. 11 Antibodies inhibit proliferation of SNU-5 cells.
- FIG. 12 Results of ADCC killing experiments of antibodies on Hs746T cells.
- FIG. 13 The results of ADCC killing experiments of antibodies on H292 cells.
- FIG. 14 Antibodies inhibit the growth of HCC827 mouse tumor cells.
- HGFR should be understood broadly and is intended to cover various forms of HGFR molecules at various stages in mammals, such as but not limited to molecules produced during the amplification, replication, transcription, splicing, processing, translation, and modification of the HGFR gene (e.g., precursor HGFR, mature HGFR, membrane-expressed HGFR, HGFR splice variants, modified HGFR, or fragments thereof); the term also covers artificially prepared or in vitro expressed HGFR.
- EGFR should be understood broadly and is intended to cover various forms of EGFR molecules at various stages in mammals, such as but not limited to molecules produced during the amplification, replication, transcription, splicing, processing, translation, and modification of the EGFR gene (e.g., precursor EGFR, mature EGFR, membrane-expressed EGFR, EGFR splice variants, modified EGFR, or fragments thereof); the term also covers artificially prepared or in vitro expressed EGFR.
- amino acid refers to naturally occurring and synthetic amino acids, as well as amino acid analogs and amino acid mimetics that function in a manner similar to naturally occurring amino acids.
- Naturally occurring amino acids are those amino acids encoded by the genetic code, as well as those amino acids that are later modified, such as hydroxyproline, ⁇ -carboxyglutamate, and O-phosphoserine.
- Amino acid analogs refer to compounds that have the same basic chemical structure (i.e., an alpha carbon bound to a hydrogen, carboxyl group, amino group, and R group) as naturally occurring amino acids, such as homoserine, norleucine, methionine sulfoxide, methionine methylsulfonium.
- Such analogs have modified R groups (e.g., norleucine) or modified peptide backbones, but retain the same basic chemical structure as naturally occurring amino acids.
- Amino acid mimetics refer to chemical compounds that have a structure that is different from the general chemical structure of an amino acid, but function in a manner similar to naturally occurring amino acids.
- antibody is used in the broadest sense and covers various antibody structures, including but not limited to monoclonal antibodies, polyclonal antibodies; monospecific antibodies, multispecific antibodies (e.g., bispecific antibodies), full-length antibodies, and antibody fragments (or antigen-binding fragments, or antigen-binding portions), as long as they exhibit the desired antigen-binding activity.
- Native antibodies refer to naturally occurring immunoglobulin molecules. For example, natural IgG antibodies are heterotetrameric proteins of about 150,000 daltons, consisting of two light chains and two heavy chains bound by disulfide bonds.
- each heavy chain has a variable region (VH), also known as a variable heavy domain, a heavy chain variable region, followed by three constant domains (CH1, CH2, and CH3).
- VH variable region
- VL variable region
- each light chain has a variable region (VL), also known as a variable light domain, or a light chain variable domain, followed by a constant light domain (light chain constant region, CL).
- bispecific antibody refers to an antibody (including an antibody or its antigen-binding fragment, such as a single-chain antibody) that can specifically bind to two different antigens or two different antigenic epitopes of the same antigen.
- the prior art has disclosed bispecific antibodies of various structures. According to the integrity of the IgG molecule, it can be divided into IgG-like bispecific antibodies and antibody fragment-type bispecific antibodies. According to the number of antigen-binding regions, it can be divided into bispecific antibodies of divalent, trivalent, tetravalent or more valence. According to whether the structure is symmetrical, it can be divided into bispecific antibodies of symmetrical structure. And asymmetric bispecific antibodies.
- fragment-type bispecific antibodies such as Fab fragments lacking Fc fragments
- Fab fragments are formed by combining two or more Fab fragments into one molecule to form bispecific antibodies, which have low immunogenicity, small molecular weight, and high tumor tissue permeability.
- Typical antibody structures of this type include F(ab′)2, scFv-Fab, (scFv)2-Fab, etc.
- IgG-like bispecific antibodies (for example, with Fc fragments) have a relatively large molecular weight.
- the Fc fragment helps purify the antibody and improve its solubility and stability.
- the Fc part may also bind to the receptor FcRn to increase the antibody serum half-life.
- Typical bispecific antibody structural models include KiH, CrossMAb, Triomab quadroma, Fc ⁇ Adp, ART-Ig, BiMAb, Biclonics, BEAT, DuoBody, Azymetric, XmAb, 2:1TCBs, 1Fab-IgG TDB, FynomAb, two-in-one/DAF, scFv-Fab-IgG, DART-Fc, LP-DART, CODV-Fab-TL, HLE-BiTE, F(ab)2-CrossMAb, IgG-(scFv)2, Bs4Ab, DVD-Ig, Tetravalent-DART-Fc, (scFv)4-Fc, CODV-Ig, mAb2, F(ab)4-CrossMAb and other bispecific antibodies (see Aran F.Labrijn et al., Nature Reviews Drug Discovery volume 18, pages 585–608 (2019); Chen S1 et al., J Immunol Res
- variable region refers to the domain that binds to the antigen in an antigen binding molecule.
- the heavy chain variable region in the antigen binding module 1 that specifically binds to HGFR is labeled M-VH, and the light chain variable region is labeled M-VL;
- the heavy chain variable region in the antigen binding module 2 that specifically binds to EGFR is labeled E-VH, and the light chain variable region is labeled E-VL.
- VH and VL each contain four conserved framework regions (FRs) and three complementary determining regions (CDRs).
- CDR complementarity determining region
- framework or “FR” refers to the variable domain residues other than the CDR residues.
- VH contains 3 CDR regions: HCDR1, HCDR2, and HCDR3;
- VL contains 3 CDR regions: LCDR1, LCDR2, and LCDR3.
- each VH and VL is composed of three CDRs and four FRs arranged from the amino terminus to the carboxyl terminus in the following order: FR1, CDR1, FR2, CDR2, FR3, CDR3, FR4.
- a single VH or VL may be sufficient to confer antigen binding specificity.
- the amino acid sequence boundaries of CDRs can be determined by various well-known schemes, for example: “Kabat” numbering rules (see Kabat et al. (1991), “Sequences of Proteins of Immunological Interest", 5th edition, Public Health Service, National Institutes of Health, Bethesda, MD), “Chothia” numbering rules, “ABM” numbering rules, "contact” numbering rules (see Martin, ACR. Protein Sequence and Structure Analysis of Antibody Variable Domains [J]. 2001) and ImMunoGenTics (IMGT) numbering rules (Lefranc, MP et al., Dev. Comp. Immunol., 27, 55-77 (2003); Front Immunol. 2018 Oct 16; 9: 2278), etc.; the correspondence between various numbering systems is well known to those skilled in the art.
- the numbering rules of the present disclosure are shown in Table 1 below.
- variable region and CDR sequences in the disclosed embodiments are all subject to the "Kabat” numbering convention.
- Kabat numbering convention is used to define amino acid residues, the corresponding technical solutions of other numbering systems will be regarded as equivalent technical solutions.
- antibody fragment refers to a molecule other than an intact antibody, which comprises a portion of an intact antibody that retains the antigen binding ability of the intact antibody.
- antibody fragments include, but are not limited to, Fv, Fab, Fab', Fab'-SH, F(ab') 2 , single domain antibodies, single chain Fab (scFab), diabodies, linear antibodies, single chain antibody molecules (e.g., scFv); and multispecific antibodies formed from antibody fragments.
- Fab refers to a protein composed of VH and CH1 (Fab heavy chain) and VL and CL (Fab light chain) of immunoglobulin.
- Fv refers to the antigen binding domain composed of VH and VL of immunoglobulin.
- the first Fab has a structure of Fab.
- CH1 and CL are replaced by titin chain or obscurin chain, respectively.
- Fc region or “fragment crystallizable region” is used to define the C-terminal region of the antibody heavy chain, including native Fc regions and modified Fc regions.
- the Fc region comprises two identical or different subunits.
- the Fc region of the human IgG heavy chain is defined as extending from the amino acid residue at the Cys226 position or from Pro230 to its carboxyl terminus.
- Suitable native sequence Fc regions for the antibodies described herein include human IgG1, IgG2 (IgG2A, IgG2B), IgG3 and IgG4. Unless otherwise indicated, the numbering convention for the Fc region is the EU index.
- Tiin chain refers to a fragment of 78-118 amino acids in the titin protein, which contains a peptide fragment of the titin Ig-like 152 domain or a functional variant thereof.
- the titin chain is capable of forming a dimerization complex with the obscurin chain.
- the term "Obscurin chain” refers to a fragment of 87-117 amino acids on the Obscurin protein, which contains a peptide segment of the Obscurin Ig-like 1 domain or a functional variant thereof; or refers to a fragment of 78-118 amino acids on the Obscurin-like 1 protein, which contains a peptide segment of the Obscurin-like Ig-like 1 domain or a functional variant thereof.
- the Obscurin chain can bind to the Titin chain to form a dimerization complex.
- the Titin chain and the Obscurin chain disclosed herein can be used to replace CH1 and CL in Fab to form a replaced Fab (Fab-S). This replacement does not affect the binding of the antigen binding molecule to the antigen.
- antigen binding moiety refers to a polypeptide molecule that specifically binds to a target antigen.
- Antigen binding moieties include antibodies and fragments thereof as described herein.
- Specific antigen binding moieties include the antigen binding domain of an antibody, which includes an antibody heavy chain variable region and an antibody light chain variable region.
- antigen binding moiety that specifically binds to HGFR refers to a module that can bind to HGFR or its epitope with sufficient affinity.
- the antigen binding moiety that specifically binds to HGFR has the following equilibrium dissociation constant (KD): ⁇ about 1 ⁇ M, ⁇ about 100 nM or ⁇ about 10 nM or less, which is measured by the Biacore method.
- the antigen binding moiety that specifically binds to HGFR binds to conserved epitopes in HGFR from different species.
- the term "antigen binding moiety that specifically binds to EGFR” refers to a module that can bind to EGFR or its epitope with sufficient affinity, so that the molecule containing the module can be used as a diagnostic agent and/or therapeutic agent targeting EGFR.
- the antigen binding moiety that specifically binds to EGFR has the following equilibrium dissociation constant (KD): ⁇ about 1 ⁇ M, ⁇ about 100 nM, ⁇ about 10 nM or less, which is measured by the Biacore method.
- the antigen binding moiety that specifically binds to EGFR binds to a conserved epitope in EGFR from different species.
- the antigen binding moiety includes an antibody fragment as defined herein, such as a Fab, a replaced Fab or a Fv.
- linker refers to a connecting unit that connects two polypeptide fragments.
- the linker can be a peptide linker, which contains one or more amino acids, typically about 1-30, 2-24 or 3-15 amino acids.
- the linkers used in this article can be the same or different.
- variable region and CDR sequences in the disclosed embodiments are all subject to the "Kabat" numbering convention.
- a “pharmaceutical composition” means a composition containing one or more bispecific antibodies described herein, as well as other components such as physiologically/pharmaceutically acceptable carriers and excipients.
- the purpose of a pharmaceutical composition is to facilitate administration to an organism, facilitate the absorption of the active ingredient, and thereby exert biological activity.
- pharmaceutical composition and “preparation” are not mutually exclusive.
- an "effective amount” includes an amount sufficient to improve or prevent the symptoms or conditions of a medical condition.
- An effective amount also means an amount sufficient to allow or facilitate diagnosis.
- the effective amount for a particular subject or veterinary subject may vary depending on factors such as the condition to be treated, the subject's overall health, the method, route and dosage of administration, and the severity of side effects.
- An effective amount can be the maximum dose or dosage regimen that avoids significant side effects or toxic effects.
- “Pharmaceutically acceptable carrier” or “pharmaceutically acceptable excipient” includes any material that, when combined with an active ingredient, allows the ingredient to retain biological activity and does not react with the subject's immune system. Examples include, but are not limited to, any standard pharmaceutical carrier, such as phosphate-buffered saline solution, water, emulsions such as oil/water emulsions, and various types of wetting agents.
- the diluent for aerosol or parenteral administration is phosphate buffered saline (PBS) or physiological (0.9%) saline.
- PBS phosphate buffered saline
- Compositions containing such carriers are formulated by well-known conventional methods.
- Buffer refers to a buffer that tolerates pH changes through the action of its acid-base conjugate components.
- buffers that control pH in an appropriate range include acetate, succinate, gluconate, histidine, oxalate, lactate, phosphate, citrate, tartrate, fumarate, glycylglycine, and other organic acid buffers.
- Hetidine buffer is a buffer containing histidine.
- histidine buffers include histidine-histidine hydrochloride, histidine-histidine acetate, histidine-histidine phosphate, histidine-histidine sulfate and the like, preferably histidine-histidine hydrochloride buffer.
- Histidine-histidine hydrochloride buffer can be prepared from histidine and hydrochloric acid, or from histidine and histidine hydrochloride.
- citrate buffer is a buffer including citrate ions.
- citrate buffers include citric acid-sodium citrate, citric acid-potassium citrate, citric acid-calcium citrate, citric acid-magnesium citrate, and the like.
- a preferred citrate buffer is citric acid-sodium citrate.
- succinate buffer is a buffer comprising succinate ions.
- succinate buffers include succinic acid-sodium succinate, succinic acid-potassium succinate, succinic acid-calcium succinate, and the like.
- a preferred succinate buffer is succinic acid-sodium succinate.
- the succinic acid-sodium succinate may be prepared from succinic acid and sodium hydroxide, or from succinic acid and sodium succinate.
- Phosphate buffer is a buffer including phosphate ions.
- phosphate buffers include citric acid-disodium hydrogen phosphate, disodium hydrogen phosphate-sodium dihydrogen phosphate, disodium hydrogen phosphate-potassium dihydrogen phosphate, disodium hydrogen phosphate-citric acid, and the like.
- a preferred phosphate buffer is citric acid-disodium hydrogen phosphate.
- Acetate buffer is a buffer including acetate ions.
- acetate buffers include acetate-sodium acetate, histidine-histidine acetate, acetate-potassium acetate, acetate-calcium acetate, acetate-magnesium acetate, etc.
- a preferred acetate buffer is acetate-sodium acetate.
- Polyxamer is a block copolymer of ethylene oxide and propylene oxide that is water-soluble and used as a surfactant in pharmaceutical formulations.
- poloxamers include poloxamer 188 (P188).
- “Lyophilized preparation” refers to a pharmaceutical composition in liquid or solution form or a preparation or pharmaceutical composition obtained after a liquid or solution preparation has been subjected to a vacuum freeze drying step.
- freeze drying includes pre-freezing, primary drying, and secondary drying.
- the purpose of pre-freezing is to freeze the product and obtain a crystalline solid; in some embodiments, the pre-freezing temperature is set to -45°C and the pre-freezing rate is set to 1°C/min.
- Primary drying also known as main drying, is the main stage of sample freeze drying.
- the temperature of the primary drying may be a conventional temperature in the art, such as -30°C-0°C.
- Secondary drying also known as analytical drying, is the main step of removing bound water in the product by drawing an extreme vacuum (0.01 mbar) and raising the temperature (20°C-40°C). Since most biological products are sensitive to temperature, secondary drying The temperature is often selected at the low end of the temperature range, such as 25° C.
- the freeze drying time is related to the freezer, the freeze-dried preparation dosage, and the freeze-dried preparation container. Such time adjustment is well known to those skilled in the art.
- the pharmaceutical composition described in the present disclosure is in the form of a solution, and unless otherwise specified, the solvent therein is water.
- the pharmaceutical composition described in the present disclosure can achieve a stabilizing effect: the antibody therein substantially retains its physical stability and/or chemical stability and/or biological activity after storage.
- the pharmaceutical composition substantially retains its physical and chemical stability and its biological activity after storage.
- the storage period is generally selected based on the predetermined shelf life of the pharmaceutical composition.
- analytical techniques for measuring protein stability which can measure stability after storage at a selected temperature for a selected period of time.
- Replacement refers to the replacement of the solvent system that dissolves the antibody protein, for example, using the buffer system of the stable preparation to replace the high-salt or hypertonic solvent system containing the antibody protein by physical manipulation, so that the antibody protein is present in the stable preparation.
- the so-called physical manipulation includes but is not limited to ultrafiltration, dialysis or centrifugation.
- a stable formulation is one in which no significant changes are observed when stored at refrigerated temperatures (2-8°C) for at least 3 months, preferably 6 months, more preferably 1 year, and even more preferably up to 2 years.
- a stable liquid formulation includes a liquid formulation that exhibits desired characteristics after being stored at a temperature of 25°C for a period of time including 24 hours, 7 days, 1 month, 3 months or 6 months. It also includes a temperature of 40°C that exhibits desired characteristics after being stored for a period of time including 4 weeks, 1 month, 3 months or 6 months.
- the formulation is a pale yellow, nearly colorless, clear liquid or a colorless, clear liquid, or clear to slightly milky white.
- concentration, pH and weight-gram molecular osmotic pressure concentration of the formulation have no more than ⁇ 10% variation, preferably no more than ⁇ 5% variation.
- the formulation usually forms no more than about 10%, preferably no more than about 5% aggregation.
- An antibody "retains its physical stability" in a pharmaceutical formulation if it shows no significant increase in aggregation, precipitation, and/or denaturation as measured after visual inspection of color and/or clarity, or by UV light scattering, size exclusion chromatography (SEC), and dynamic light scattering (DLS). Changes in protein conformation can be assessed by fluorescence spectroscopy, which determines protein tertiary structure, and by FTIR spectroscopy, which determines protein secondary structure.
- An antibody "retains its chemical stability" in a pharmaceutical formulation if it shows no significant chemical changes. Chemical stability can be assessed by detecting and quantifying chemically altered forms of the protein.
- Degradation processes that often change the chemical structure of a protein include hydrolysis or truncation (assessed by methods such as size exclusion chromatography and CE-SDS), oxidation (assessed by methods such as peptide mapping in combination with mass spectrometry or MALDI/TOF/MS), deamidation (assessed by methods such as ion exchange chromatography, capillary isoelectric focusing, peptide mapping, isoaspartate measurement), and isomerization (assessed by measuring isoaspartate content, peptide mapping, etc.).
- An antibody "retains its biological activity" in a pharmaceutical formulation if the biological activity of the antibody at a given time is within a predetermined range of the biological activity exhibited when the pharmaceutical formulation is prepared.
- administering when applied to animals, humans, experimental subjects, cells, "Administer,””administer,” and “treat” refer to, for example, contact of an exogenous drug, therapeutic agent, diagnostic agent, or composition with an animal, human, subject, cell, tissue, organ, or biological fluid.
- administering may refer to, for example, treatment, pharmacokinetics, diagnosis, research, and experimental procedures.
- Treatment of cells includes contact of an agent with a cell, and contact of an agent with a fluid, wherein the fluid is in contact with the cell.
- administer also mean in vitro and ex vivo treatment of, for example, a cell, by an agent, a diagnosis, a combination composition, or by another cell.
- Treat as it applies to humans, veterinary medicine, or research subjects, refers to therapeutic treatment, prophylactic or preventive measures, research and diagnostic applications.
- Treatment means administering an internal or external therapeutic agent, such as a reconstituted solution containing any of the present disclosure, to a patient who has one or more symptoms of a disease for which the therapeutic agent is known to have a therapeutic effect.
- the therapeutic agent is administered in an amount effective to alleviate one or more symptoms of the disease in the treated patient or population to induce regression of such symptoms or inhibit the development of such symptoms to any clinically measurable degree.
- the amount of therapeutic agent effective to alleviate any specific disease symptom may vary according to a variety of factors, such as the patient's disease state, age, and weight, and the ability of the drug to produce the desired therapeutic effect in the patient.
- Whether the disease symptom has been alleviated can be evaluated by any clinical test method commonly used by doctors or other professional health care personnel to evaluate the severity or progression of the symptom.
- the embodiments of the present disclosure e.g., treatment methods or products
- may not be effective in alleviating every target disease symptom they should alleviate the target disease symptoms in a statistically significant number of patients as determined by any statistical test known in the art, such as Student's t-test, chi-square test, U test according to Mann and Whitney, Kruskal-Wallis test (H test), Jonckheere-Terpstra test, and Wilcoxon test.
- the reconstituted solution of the present disclosure can be applied by any suitable means, including parenteral, intrapulmonary and intranasal, and if local treatment is required, intralesional application.
- Parenteral infusion includes intramuscular, intravenous, intraarterial, intraperitoneal or subcutaneous administration. Administration can be by any appropriate route, for example, by injection, such as intravenous or subcutaneous injection.
- a variety of dosing time schemes are contemplated herein, including but not limited to, single or multiple administrations at multiple time points, push administration and pulse infusion.
- the reconstituted solution of the present disclosure is administered by subcutaneous injection.
- the reconstituted solution of the present disclosure will be formulated, dosed and applied in a manner consistent with good medical practice.
- Factors to be considered in this context include the specific condition being treated, the specific mammal being treated, the clinical condition of the individual patient, the cause of the condition, the delivery site of the agent, the method of administration, the administration schedule, and other factors known to medical practitioners.
- the reconstituted solution can also be formulated with one or more other agents for preventing or treating the condition. The effective amount of such other agents depends on the amount of antigen binding molecules present in the reconstituted solution, the type of condition or treatment, and other factors.
- PCT/CN2022/105714 (application date: 2022.07.14; priority patent application number: CN202110794137.9) is incorporated into this disclosure in its entirety by reference.
- Human EGFR protein (UniProt Epidermal growth factor receptor, UniProt No.: P00533) was used as a template for EGFR, and a His tag was fused to the EGFR protein to design the extracellular domain of human EGFR protein with a His tag (abbreviated as: EGFR-His) for detection in the present invention; its amino acid sequence is as follows:
- the underlined part is the extracellular domain of EGFR protein; the italic part is the His tag.
- HGFR protein Human HGFR protein (UniProt Hepatocyte growth factor receptor, Uniprot No.: P08581) was used as a template for HGFR, and a His tag or Fc was fused to the HGFR protein to design the HGFR protein extracellular domain with a His tag (abbreviated as HGFR-His) and the HGFR protein extracellular domain with an Fc tag (abbreviated as HGFR-Fc) for detection in the present invention, and the amino acid sequences thereof are as follows:
- the underlined part is the extracellular domain of HGFR protein; the italic part is the His tag.
- HGF-His HGF-His
- the underlined part is HGF protein; the italic part is His tag.
- the cell expression supernatant sample was centrifuged at high speed to remove impurities, and the buffer was replaced with PBS, and imidazole was added to a final concentration of 5mM.
- the nickel column was equilibrated with a PBS solution containing 5mM imidazole and washed 2-5 times the column volume.
- the replaced cell supernatant sample was loaded onto a Ni Sepharose excel column (GE, 17-3712-02).
- the column was washed with a PBS solution containing 5mM imidazole until the A280 reading dropped to the baseline.
- the column was then washed with PBS+10mM imidazole to remove non-specifically bound impurities and collect the effluent.
- the target protein was then eluted with a PBS solution containing 300mM imidazole, and the elution peak was collected.
- the collected eluate was concentrated and further purified using gel chromatography Superdex200 (GE, 28-9893-35) with PBS as the mobile phase.
- the aggregate peak was removed and the elution peak was collected.
- the obtained protein was identified as correct by electrophoresis, peptide mapping, and LC-MS and then allocated for use.
- the supernatant sample expressed by the cells was subjected to high-speed centrifugation to remove impurities, and the supernatant was subjected to MabSelect Sure (GE, 17-5438-01) affinity chromatography.
- the MabSelect Sure chromatography column was first regenerated with 0.2M NaOH, rinsed with pure water, and then balanced with PBS. After the supernatant was combined, it was washed with PBS until the A280 reading dropped to the baseline.
- the target protein was eluted with 0.1M acetate buffer at pH 3.5 and neutralized with 1M Tris-HCl.
- the eluted sample was appropriately concentrated, it was further purified using gel chromatography Superdex200 (GE, 28-9893-35) balanced with PBS, and the receiving tube where the target protein was located was collected and concentrated to an appropriate concentration. This method is used to purify the antibodies involved in the present invention.
- Antibodies that specifically bind to HGFR and EGFR are constructed by using molecules that specifically bind to HGFR and molecules that specifically bind to EGFR.
- the molecule that specifically binds to EGFR can be derived from any suitable antibody, such as Zalutumumab (abbreviated as Zal) or its variants (such as Zal.1, Zal.1 is obtained by mutating the first amino acid residue of the Zal light chain from A to D), wherein:
- amino acid sequence of the heavy chain variable region of Zal.1 is SEQ ID NO: 3
- the molecules that specifically bind to HGFR can be derived from any suitable antibodies, including antibodies such as Onartuzumab (Omab) described in International Publication No. WO2013003680A1 (incorporated herein by reference in its entirety); antibodies such as Ab10 described in International Publication No. WO2016165580A1 (incorporated herein by reference in its entirety); and mutant antibodies of the above antibodies.
- the amino acid sequences of the variable regions of Omab and Ab10 are as follows:
- the underlined part is the CDR region confirmed according to the Kabat numbering system, and the italic bold part is the mutated amino acid residue.
- a bispecific antibody having a Format 1 or Format 2 structure that specifically binds to HGFR and EGFR is constructed by using a molecule that specifically binds to HGFR and a molecule that specifically binds to EGFR.
- the schematic diagram of the structure of Format 1 is shown in FIG1
- the schematic diagram of the structure of Format 2 is shown in FIG2 .
- Format1 contains 4 chains with the following structure:
- Chain 1 is:
- VH anti-EGFR antibody
- linker 1-VH anti-HGFR antibody 1-IgG 1 (CH1)-IgG 1 Fc (knob)
- Chain 2 is: VL (anti-EGFR antibody)-Linker 1-VL (anti-HGFR antibody 1)-CL,
- Chain 3 is: VH (anti-HGFR antibody 2)-Linker 2-Titin chain-IgG 1 Fc (hole),
- Chain 4 is: VL (anti-HGFR antibody 2)-Linker 2-Obscurin chain.
- Format2 contains 4 chains with the following structure:
- Chain 1 is: VH (anti-HGFR antibody)-IgG 1 (CH1)-IgG 1 Fc (knob),
- Chain 2 is: VL (anti-HGFR antibody)-CL,
- Chain 3 is: VH (anti-EGFR antibody)-Linker 2-Titin chain-IgG 1 Fc (hole),
- Chain 4 is: VL (anti-EGFR antibody)-Linker 2-Obscurin chain;
- EM1 having a Format1 structure and EM2 having a Format2 structure were constructed using Ab10.1 (anti-HGFR antibody 1), Omab (anti-HGFR antibody 2), Zal.1 (anti-EGFR antibody), human IgG 1 heavy chain constant region/kappa light chain constant region, and T.16 (Titin chain)/O.28 (Obscurin chain).
- amino acid sequences of the four chains of EM1 are as follows:
- amino acid sequences of the four chains of EM2 are shown below:
- the single underlined part is the variable region
- the dotted underlined part is the CL
- the dotted underlined part is the Fc region
- the italic part is CH1
- the double underlined part is the linker
- the wavy line part is the Obscurin chain/Titin-T chain.
- linker 1 GGGGSGGGG (SEQ ID NO: 45)
- Test Example 1 Biacore detection of in vitro binding affinity and kinetics experiments
- the affinity of the test molecule to human EGFR (SEQ ID NO: 42) or human HGFR protein (SEQ ID NO: 43) was determined by Biacore T200 (GE) as follows:
- the antibody molecules were captured by affinity using a Protein A biosensor chip, and then the antigen was passed through the chip surface for 180 seconds, followed by dissociation for 600 seconds.
- the Biacore T200 instrument was used to detect the reaction signal in real time to obtain the binding and dissociation curves. After each experimental cycle of dissociation was completed, the biosensor chip was washed and regenerated with 10mM Gly-HCl (pH 1.5).
- the data fitting model used a 1:1 model to obtain the affinity value of the molecule to be tested.
- the positive control MCLA is a bispecific antibody that specifically binds to HGFR and EGFR (for the specific sequence, see PB8532 in WO2019031965A1).
- the experimental results are shown in Table 4.
- the experimental results show that the mutant antibodies and bispecific antibodies constructed in the present disclosure have very strong affinities, and the affinity of Ab10.1 is at least 4 times that of the parent antibody Ab10.
- Test Example 2 In vitro cell binding assay of antibodies
- the cell binding activity of the bispecific antibody that specifically binds to HGFR and EGFR was detected by flow cytometry.
- antibody samples diluted at different concentrations C25 is a negative control, which is an IgG1 antibody protein irrelevant to the target, the same below
- C25 is a negative control, which is an IgG1 antibody protein irrelevant to the target, the same below
- R-PE-sheep anti-human (H+L) antibody Invitrogen, CAT#H10104
- Test Example 3 Antibody blocking experiment of EGFR binding to EGF
- the blocking ability of the antibody on EGF/EGFR was detected by ELISA. Coat with 1 ⁇ g/mL rabbit anti-His antibody (GenScript, A01857) and block at 4°C overnight. After washing the plate, add 2 ⁇ g/mL EGFR-His (SEQ ID NO: 42) and incubate for 1 hour. After washing the plate, add 2 ⁇ g/mL EGF-mFc (Acro, EGF-H525b) and antibodies diluted in different concentrations and incubate for 1 hour. After washing the plate, add horseradish peroxidase-sheep anti-mouse IgG antibody (Jackson, 115-035-062) and incubate for 1 hour.
- the positive control JNJ is a bispecific antibody that specifically binds to HGFR and EGFR (JNJ is also called Amivantamab, for the specific sequence structure, see WHO Drug Information, 33 (2): 237-239).
- the experimental results are shown in Table 5.
- the experimental results show that the bispecific antibody specifically binding to HGFR and EGFR disclosed herein can effectively block the binding of EGF to EGFR.
- Test Example 4 Antibody antagonism of HGFR binding to HGF blocking experiment
- the blocking ability of the antibody on HGF/HGFR was detected by ELISA. Coat with 5 ⁇ g/mL HGFR-His (sequence see SEQ ID NO: 43) and block at 4°C overnight. After washing, add 2 ⁇ g/mL bio-HGF-His (sequence see SEQ ID NO: 44) and antibodies diluted in different concentrations and incubate for 1 hour. After washing, add horseradish peroxidase-streptavidin (Jackson, 016-030-084) and incubate for 1 hour. After washing, add tetramethylbenzidine solution for color development, and finally add stop solution, and measure the OD450 value on the microplate reader.
- H292 cells also known as NCI-H292, human lung cancer cells (lymph node metastasis), Chinese Academy of Sciences Cell Bank, the same below
- the culture medium was RPMI1640 + 10% FBS, and then the 96-well plates were cultured in a 37°C, 5% CO2 incubator for 24 hours.
- the culture medium was discarded and replaced with serum-free culture medium (RPMI640 + 25mM HEPES + 0.1mM NEAA + 1mM sodium pyruvate) for starvation for 16-20 hours.
- serum-free culture medium RPMI640 + 25mM HEPES + 0.1mM NEAA + 1mM sodium pyruvate
- the antibody was prepared with an initial concentration of 6 ⁇ M, and diluted 5-fold.
- the starvation medium was replaced and cultured in a 37°C, 5% CO2 incubator for 1 hour. During this period, 50 ⁇ L of 100 ng/mL rEGF (R&D, 236-EG-200) was added and cultured in a 37°C, 5% CO2 incubator for 15 minutes. Finally, phosphorylated EGFR was detected according to the instructions of PHOSPHO-EGFR (TYR1068) KITS (Cisbio, 64EG1PEG).
- FIG7 The experimental results are shown in FIG7 , which show that the bispecific antibody disclosed herein that specifically binds to HGFR and EGFR can effectively inhibit cellular EGFR phosphorylation.
- H292 cells were plated in a 96-well plate (Corning, 3599) at 12,000 cells/well in a medium of RPMI1640 + 10% FBS. The 96-well plate was then incubated in a 37°C, 5% CO 2 incubator for 24 hours. On the first day, the culture medium was discarded and replaced with serum-free culture medium (RPMI640+25mM HEPES+0.1mM NEAA+1mM sodium pyruvate) for starvation for 16-20 hours. On the third day, the antibody was prepared with an initial concentration of 1 ⁇ M, 10-fold gradient dilution, and the starvation culture medium was replaced.
- serum-free culture medium RPMI640+25mM HEPES+0.1mM NEAA+1mM sodium pyruvate
- the cells were cultured in a 37°C, 5% CO 2 incubator for 1 hour, during which 50 ⁇ L 200ng/mL rHGF (R&D, 294-HG-005) was added and cultured in a 37°C, 5% CO 2 incubator for 30 minutes. Finally, phosphorylated HGFR was detected according to the instructions of pc-Met (Tyr1234/1235) Assay Kit (PerkinElmer, ALSU-PCMET-A50).
- FIG8 The experimental results are shown in FIG8 , which show that the bispecific antibody disclosed herein that specifically binds to HGFR and EGFR can effectively inhibit cellular HGFR phosphorylation, and its inhibitory ability is stronger than that of Ab10.1.
- H292 cells were plated in 96-well plates (Corning, 3599) at 12,000 cells/well in RPMI1640+10% FBS medium, and then the 96-well plates were cultured in a 37°C, 5% CO2 incubator for 24 hours. The next day, the medium was discarded and replaced with serum-free medium (RPMI640+25mM HEPES+0.1mM NEAA+1mM sodium pyruvate) for starvation for 16-20 hours. On the third day, the antibody was prepared with an initial concentration of 4 ⁇ M, and then diluted 5 times in a gradient, and the starvation medium was replaced.
- serum-free medium RPMI640+25mM HEPES+0.1mM NEAA+1mM sodium pyruvate
- the cells were cultured in a 37°C, 5% CO 2 incubator for 1 hour, during which 50 ⁇ L of 200 ng/mL rHGF (R&D, 294-HG-005) and 50 ng/mL rEGF (R&D, 236-EG-200) were added, mixed evenly, and cultured in a 37°C, 5% CO 2 incubator for another 1 hour.
- phosphorylated AKT was detected according to the instructions of PHOSPHO-AKT (SER473) KITS (Cisbio, 64AKSPEG).
- the intracellular AKT phosphorylation level is reflected by the ratio of the fluorescence signal value at 665 nm to the fluorescence signal value at 620 nm.
- the ratio fluorescence signal value at 665 nm/fluorescence signal value at 620 nm * 10 4 .
- the higher the ratio the higher the intracellular AKT phosphorylation level.
- FIG9 show that the bispecific antibody disclosed herein that specifically binds to HGFR and EGFR can effectively inhibit cellular AKT phosphorylation, and the inhibitory ability is stronger than that of the positive control antibody.
- Test Example 8 Antibodies reduce HGFR expression on the cell surface
- HCC827 cells human non-small cell lung cancer cells, from ATCC
- HCC827 cells were plated in 6-well plates at 3 ⁇ 10 5 /well in RPMI1640+10% FBS culture medium, and then the 6-well plates were cultured in a 37°C, 5% CO 2 incubator for 24 hours.
- Antibodies were prepared to a concentration of 200 nM, treated and reacted for 18 hours. After treatment, the cells were washed once with PBS, 500 ⁇ L of enzyme-free cell dissociation buffer (Gibco, #13151) was added to collect the cells, and the cells were separated from the enzyme-free cell dissociation buffer by centrifugation.
- enzyme-free cell dissociation buffer Gibco, #13151
- Immunofluorescence staining was performed using goat anti-human HGFR antibody (R&D systems, AF276) and reacted at 4°C for 1 hour. Subsequently, the cells were washed twice with PBS containing 2% FBS. ALEXA488-donkey anti-goat secondary antibody (Thermo Fisher, A-11055) was then added to react at 4°C for 1 hour, and the cells were fixed with BD Cytofix (BD, #554655) after washing twice. After washing once with PBS, the fluorescence signal value was read using a flow cytometer.
- the experimental results are shown in Figure 10.
- the experimental results show that compared with the positive control antibody, the bispecific antibody disclosed herein that specifically binds to HGFR and EGFR can more effectively reduce HGFR on the cell surface and inhibit receptor signaling. path.
- SNU-5 cells human gastric cancer cells, from ATCC
- SNU-5 cells human gastric cancer cells, from ATCC
- the 96-well plate was then incubated at 37°C, 5% CO 2 incubator for 24 hours.
- the antibody was prepared to a concentration of 5 ⁇ M, diluted 5-fold, added to the above 96-well plate, and then incubated at 37°C, 5% CO 2 incubator for 5 days. Finally, the antibody was prepared according to the instructions.
- Luminescent Cell Viability Assay Promega, G7573
- Lum measured value - culture medium reading
- % inhibition (Lum0-Lum)/Lum0*100%
- Lum0 is the fluorescence intensity of the well with an antibody concentration of 0
- Lum is the fluorescence intensity of the wells with antibodies at various dilution concentrations.
- FIG11 The experimental results are shown in FIG11 , which show that the bispecific antibody disclosed herein that specifically binds to HGFR and EGFR has a stronger ability to inhibit the proliferation of SNU-5 cells than the positive antibody.
- Test Example 10 Antibody killing experiment on tumor cells by ADCC
- Hs746T cells human gastric cancer cells, Nanjing Kebai Biotechnology Co., Ltd.
- H292 cells wash them once with PBS, adjust the cell density to 1 ⁇ 10 6 cells/mL with buffer (PBS+10% FBS+20mM HEPES), then add 3 ⁇ L BATDA reagent (PerkinElmer, AD0116) to 2mL of cells and incubate them at 37°C, 5% CO 2 incubator for 15min. After centrifugation, wash three times with wash buffer (PBS+20mM HEPES), count and adjust the cell density to 1 ⁇ 10 5 cells/mL.
- NK92--FCGR3A (176V) cells NK92 cells stably transformed with FCGR3A (176V) (purchased from Nanjing Kebai Biotechnology Co., Ltd.)
- FCGR3A (176V) purchased from Nanjing Kebai Biotechnology Co., Ltd.
- 100 ⁇ L NK92--FCGR3A (176V) cells, 50 ⁇ L diluted antibody, and 50 ⁇ L labeled tumor cells were added to a 96-well plate (corning, 3788).
- a spontaneous control release well was set up, which did not contain NK92--FCGR3A (176V) cells but contained 100 ⁇ L culture medium; a positive control well was set up, which contained 10 ⁇ L lysis buffer + 50 ⁇ L labeled tumor cells + 140 ⁇ L culture medium; a background control well was set up, which contained 50 ⁇ L of labeled cell supernatant + 150 ⁇ L culture medium. Then the 96-well plate was centrifuged at 300 rpm for 2 minutes and incubated in a 37°C, 5% CO 2 incubator for 2 hours.
- EM1 (afuc) is defucosylated EM1; EM2 (afuc) is defucosylated EM2.
- Test Example 11 In vivo efficacy experiment of antibody in mouse H1975-HGF model
- H1975-HGF cells (H1975 cells (human lung adenocarcinoma cells, ATCC) stably transfected with human HGF) (5 ⁇ 10 6 cells) were inoculated subcutaneously in the right flank of CD1 nude female mice (Viton River).
- the animals were randomly divided into groups according to the tumor volume, with 10 animals in each group, including C25-3mpk group (negative control, which is an IgG1 antibody protein of an irrelevant target, and the dosage is 3 mg/kg), JNJ-3mpk group (positive control, the dosage is 3 mg/kg) and EM1-3.5mpk group (EM1 test group, the dosage is 3.5 mg/kg).
- C25-3mpk group negative control, which is an IgG1 antibody protein of an irrelevant target, and the dosage is 3 mg/kg
- JNJ-3mpk group positive control, the dosage is 3 mg/kg
- EM1-3.5mpk group EM1 test group, the dosage is 3.5 mg/kg.
- the drug was administered twice a week by intraperitoneal injection for 2 to 3 consecutive weeks.
- the tumor volume was measured twice a week, the mouse body weight was weighed, and the data was recorded.
- the data were statistically analyzed by T-test. Among them, C25-3mpk, JNJ-3mpk,
- Relative tumor proliferation rate T/C% (T-T0)/(C-C0) ⁇ 100%;
- TGI% 1-T/C%
- T and T0 are the tumor volumes of the drug-treated group, wherein T0 is the tumor volume at the beginning of the experiment; C and C0 are the tumor volumes of the negative control group, wherein C0 is the tumor volume of the negative control group at the beginning of the experiment.
- Test Example 12 In vivo efficacy study of antibodies in the mouse HCC827 model
- HCC827 cells human non-small cell lung cancer cells, derived from ATCC (8.555 ⁇ 10 6 cells) were inoculated subcutaneously in the right flank of NUNU female mice (Viton River).
- the animals were randomly divided into groups according to the tumor size, with 10 animals in each group, including C25-3mpk group (negative control, in which C25 was an IgG1 antibody protein of an irrelevant target, and the dosage was 3 mg/kg), MCLA-3mpk group (positive control, and the dosage was 3 mg/kg), EM1-3.5mpk group (EM1 high-dose test group, and the dosage was 3.5 mg/kg), EM1-1.17mpk group (EM1 low-dose test group, and the dosage was 1.17 mg/kg) and EM2-3mpk group (EM2 test group, and the dosage was 3 mg/kg).
- C25-3mpk group negative control, in which C25 was an IgG1 antibody protein of an irrelevant target, and the dosage was 3 mg/kg
- MCLA-3mpk group positive control, and the dosage was 3 mg/kg
- EM1-3.5mpk group EM1 high-dose test group, and the dosage was 3.5 mg/kg
- EM1-1.17mpk group EM1 low
- the drug was administered twice a week by intraperitoneal injection for 2 to 3 consecutive weeks.
- the tumor volume was measured twice a week, the mouse body weight was weighed, and the data was recorded.
- C25-3mpk, MCLA-3mpk, EM1-3.5mpk and EM2-3mpk were equimolar.
- FIG14 The experimental results are shown in FIG14 , which show that the bispecific antibody disclosed herein that specifically binds to HGFR and EGFR can significantly inhibit the growth of tumor cells, and the tumor inhibition effect is stronger than that of the positive control antibody.
- SEC% SEC monomer content percentage
- a monomer is the peak area of the main peak monomer in the sample, and A total is the sum of all peak areas).
- ⁇ SEC% SEC% of the preparation after stability experiment - SEC% of the preparation before stability experiment.
- a method of electrophoresis in which the gel is moved into a capillary tube as a supporting medium and the samples are separated according to their molecular weight at a certain voltage.
- NR-CE% Amainpeak/Atotal ⁇ 100% (Amainpeak is the peak area of the main peak in the sample, and Atotal is the sum of all peak areas).
- ⁇ NR-CE% NR-CE% of the preparation after the stability experiment-NR-CE% of the preparation before the stability experiment.
- CE measurement instrument Beckman capillary electrophoresis instrument, model PA800plus.
- a chromatographic method that uses ion exchange resins or chemically bonded ion exchangers as stationary phases and utilizes differences in ion exchange capacity or selectivity of the separated components to achieve separation.
- IEC% A neutral peak area / A total area ⁇ 100% (A total area is the sum of the acidic peak, neutral peak and basic peak areas).
- ⁇ IEC% IEC% of the preparation after stability experiment - IEC% of the preparation before stability experiment.
- the freezing point method is used to determine osmotic pressure. It is based on the fact that the freezing point depression value is proportional to the molar concentration of the solution. A highly sensitive temperature sensing element is used to determine the freezing point of the solution and convert the amount of electricity into osmotic pressure.
- the protein used in the following examples is the HGFR-EGFR bispecific antibody EM1 (hereinafter referred to as "protein").
- Instrument for determining protein concentration UV-visible spectrophotometer, model: Nano Drop oneC, optical path length: 1 mm.
- Preparations of 100 mg/mL protein, 80 mg/mL sucrose, and 0.4 mg/mL polysorbate 80 (PS80) were prepared using the buffer system shown in Table 8. Forced degradation studies were conducted on the samples (40°C for 4 weeks), and the effects of different buffer systems on protein stability were investigated using SEC and NR-CE as evaluation indicators.
- His-HCl stands for histidine-histidine hydrochloride
- 40°C W4 placed at 40°C for 4 weeks, the same below.
- Preparations of 100 mg/mL protein, 80 mg/mL sucrose, and 0.4 mg/mL polysorbate 80 (PS80) were prepared using the buffer system shown in Table 9. Forced degradation studies were conducted on the samples (40°C for 4 weeks), and the effects of different buffer systems on protein stability were investigated using appearance, SEC, and NR-CE as evaluation indicators.
- AA acetic acid-sodium acetate
- His-AA stands for histidine-histidine acetate
- CA citric acid-sodium citrate
- SA succinic acid-sodium succinate
- PB disodium hydrogen phosphate-sodium dihydrogen phosphate
- Preparations of 100 mg/mL protein, different types of sugars shown in Table 10, and 0.6 mg/mL PS80 were prepared using 20 mM His-HCl buffer (pH 6.0). The samples were subjected to forced degradation studies (40°C for 4 weeks) and the effects of different types of sugars on protein stability were investigated using appearance, SEC, and NR-CE as evaluation indicators.
- a preparation of 100 mg/mL protein, 75 mg/mL sucrose, and the surfactants shown in Table 11 was prepared using 20 mM His-HCl buffer (pH 6.0). The samples were subjected to forced degradation studies (25°C, 300 rpm shaking for 7 days; 40°C for 4 weeks), and the effects of different surfactants on protein stability were investigated using SEC and NR-CE as evaluation indicators.
- the protein samples in Table 12 were prepared and freeze-dried.
- the freeze-drying parameters are shown in Table 13:
- the proteins prepared with 20 mM His-HCl pH 5.0 and 20 mM His-HCl pH 6.0 were concentrated to different concentrations, and their viscosities at 25°C were measured. The results are shown in Table 16. In the His-HCl system, the viscosity at pH 5.0 is lower than that at pH 6.0.
- N/A means that the sample could not be prepared due to its high viscosity.
- the protein samples in Table 20 were prepared and lyophilized.
- the lyophilization parameters are shown in Table 14:
- 40°C4W means that the chamber is placed at 40°C for 4 weeks
- 2-8°CM6 means that the chamber is placed at 4°C for 6 months
- 25°CM6 means that the chamber is placed at 25°C for 6 months.
- the freeze-drying results showed that the powder cake of the freeze-dried product was intact without obvious collapse.
- the formula after reconstitution was 150 mg/mL protein concentration, 25 mM pH 5.0 His-HCl, 70 mg/mL sucrose, 10 mM methionine, 0.6 mg/mL PS80, 2000 U/mL hyaluronidase, and the appearance was clear and transparent after reconstitution.
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Immunology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Engineering & Computer Science (AREA)
- Mycology (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Abstract
本披露涉及一种包含特异性结合HGFR和EGFR的双特异性抗体的药物组合物。具体而言,本披露涉及一种包含特异性结合HGFR和EGFR的双特异性抗体的药物组合物及其作为药物的用途。
Description
本披露属于药物制剂领域,具体涉及一种包含特异性结合HGFR和EGFR的双特异性抗体的药物组合物,以及其作为药物的用途。
这里的陈述仅提供与本披露有关的背景信息,而不必然地构成现有技术。
表皮生长因子受体(EGFR)和肝细胞生长因子受体(HGFR)是两种受体酪氨酸激酶,共同在多种肿瘤细胞上高表达,如非小细胞肺癌(NSCLC),结直肠癌(CRC)和头颈癌(HNC)等。
EGFR信号通路通过调节细胞增殖,血管生成以及癌症细胞的转移和生存,在肿瘤生物学中发挥着重要作用,该通路的失调会导致肿瘤的发生。由于HGFR的过表达,活化突变,自分泌或旁分泌信号转导或者基因扩增引起的异常信号激活,HGFR也与许多肿瘤的发生相关。通过对癌症疗效的调查发现,EGFR和HGFR信号通路存在着重要联系。
NSCLC占肺癌总数的83%,而EGFR激活型突变是其中常见的类型(白人10-15%,亚洲人50%)。一直以来,EGFR酪氨酸激酶抑制剂(TKI)都是NSCLC的一线疗法。尽管初始响应率很高(70-80%),但通常在一年内就会出现耐药。耐药的机制主要有两种,其一是另一种EGFR突变的出现,比如EGFR T790M;其二就是HGFR信号通路的补偿激活。这些因素均影响肿瘤的治疗效果。
发明内容
本披露提供一种包含特异性结合HGFR和EGFR的双特异性抗体的药物组合物,该组合物具有治疗活性。此外,该组合物还具有稳定性好等优势。
在一些实施方案中,本披露提供一种药物组合物,包含特异性结合HGFR和EGFR的双特异性抗体和缓冲剂,其中:
所述特异性结合HGFR和EGFR的双特异性抗体包含至少一个特异性结合HGFR的抗原结合模块1和至少一个特异性结合EGFR的抗原结合模块2;
所述缓冲剂为组氨酸缓冲剂、醋酸盐缓冲剂、枸橼酸盐缓冲剂、琥珀酸盐缓冲剂或磷酸盐缓冲剂。
在一些实施方案中,本披露提供一种药物组合物,包含特异性结合HGFR和EGFR的双特异性抗体和缓冲剂,其中:
所述特异性结合HGFR和EGFR的双特异性抗体包含至少一个特异性结合HGFR的抗原结合模块1和至少一个特异性结合EGFR的抗原结合模块2;
所述抗原结合模块1包含重链可变区M-VH和轻链可变区M-VL,所述M-VH
包含M-HCDR1、M-HCDR2和M-HCDR3,所述M-VL包含M-LCDR1、M-LCDR2和M-LCDR3,和
所述抗原结合模块2包含重链可变区E-VH和轻链可变区E-VL,所述E-VH包含E-HCDR1、E-HCDR2和E-HCDR3,所述E-VL包含E-LCDR1、E-LCDR2和E-LCDR3,
所述E-HCDR1、E-HCDR2、E-HCDR3、E-LCDR1、E-LCDR2和E-LCDR3是根据Kabat编号规则定义的,所述E-HCDR1如SEQ ID NO:6所示,所述E-HCDR2如SEQ ID NO:7所示,所述E-HCDR3如SEQ ID NO:8所示,所述E-LCDR1如SEQ ID NO:9所示,所述E-LCDR2如SEQ ID NO:10所示,和所述E-LCDR3如SEQ ID NO:11所示;和
所述M-HCDR1、M-HCDR2、M-HCDR3、M-LCDR1、M-LCDR2和M-LCDR3是根据Kabat编号规则定义的,其中,
(i)所述M-HCDR1如SEQ ID NO:30所示,所述M-HCDR2如SEQ ID NO:31所示,所述M-HCDR3如SEQ ID NO:32所示,所述M-LCDR1如SEQ ID NO:27所示,所述M-LCDR2如SEQ ID NO:33所示,和所述M-LCDR3如SEQ ID NO:29所示,或
(ii)所述M-HCDR1如SEQ ID NO:18所示,所述M-HCDR2包含SEQ ID NO:19所示,所述M-HCDR3包含SEQ ID NO:20所示,所述M-LCDR1包含SEQ ID NO:21所示,所述M-LCDR2包含SEQ ID NO:22所示,和所述M-LCDR3包含SEQ ID NO:23所示,或
(iii)所述M-HCDR1如SEQ ID NO:24所示,所述M-HCDR2如SEQ ID NO:25所示,所述M-HCDR3如SEQ ID NO:26所示,所述M-LCDR1如SEQ ID NO:27所示,所述M-LCDR2如SEQ ID NO:28所示,和所述M-LCDR3如SEQ ID NO:29所示;
所述缓冲剂为组氨酸缓冲剂、醋酸盐缓冲剂、枸橼酸盐缓冲剂、琥珀酸盐缓冲剂或磷酸盐缓冲剂。
在一些实施方案中,所述缓冲剂为组氨酸缓冲剂。
在一些实施方案中,所述缓冲剂为组氨酸-盐酸组氨酸缓冲剂或组氨酸-醋酸组氨酸缓冲剂。
在一些具体的实施方案中,所述缓冲剂为组氨酸-盐酸组氨酸缓冲剂。
在一些实施方案中,如上任一项所述的药物组合物,其中所述药物组合物的pH为4.5至6.5。在一些实施方案中,如上任一项所述的药物组合物,其中所述药物组合物的pH为4.8至5.2。在一些实施方案中,所述药物组合物的pH为5.0至6.0。
在一些实施方案中,所述药物组合物的pH为5.0至6.5。在一些实施方案中,所述药物组合物的pH为5.5至6.5。在一些实施方案中,所述药物组合物的pH为
5.5至6.0。在一些实施方案中,所述药物组合物的pH为6.0至6.5。在一些实施方案中,如上任一项所述的药物组合物,其中所述药物组合物的pH为5.8至6.2。在一些实施方案中,所述药物组合物的pH为约5.8。在一些实施方案中,所述药物组合物的pH为约6.0。在一些实施方案中,所述药物组合物的pH为约6.2。
在一些实施方案中,如上任一项所述的药物组合物,其中所述药物组合物的pH为4.5至6.0。在一些实施方案中,如上任一项所述的药物组合物,其中所述药物组合物的pH为4.5至5.5。在一些实施方案中,如上任一项所述的药物组合物,其中所述药物组合物的pH为4.8至5.2。在一些实施方案中,所述药物组合物的pH为约4.8。在一些实施方案中,所述药物组合物的pH为约5.0。在一些实施方案中,所述药物组合物的pH为约5.2。
当本披露中提及点值时,应当理解该点值包含了误差范围。这种误差范围是由于实验室环境、人员操作、仪器、方法学、测量误差等因素所致。以pH为例,当测值为约6.0时,应当理解其包含了误差范围。作为一个示例,采用工业用pH计测量制剂时,“约6.0”表示6.0±0.2(即pH为5.8至6.2)。
在一些实施方案中,所述药物组合物的pH为4.5、4.6、4.7、4.8、4.9、5.0、5.1、5.2、5.3、5.4、5.5、5.6、5.7、5.8、5.9、6.0、6.1、6.2、6.3、6.4或6.5,或者为这些点值之间的任意范围。在一些实施方案中,所述药物组合物的pH为4.8。在一些实施方案中,所述药物组合物的pH为5.0。在一些实施方案中,所述药物组合物的pH为5.2。在一些实施方案中,所述药物组合物的pH为5.8。在一些实施方案中,所述药物组合物的pH为6.0。在一些实施方案中,所述药物组合物的pH为6.2。
通常,通过置换缓冲剂获得的药物组合物的pH与缓冲剂pH几乎一致。同时,本领域技术人员公知,在药物制剂的过程中,有时可能会存在pH飘移,但药物制剂的pH的飘移一般很小(例如±0.3范围内)。在一些实施方案中,药物制剂的pH的飘移在±0.3范围内。在一些实施方案中,药物制剂的pH的飘移在±0.2范围内。在一些实施方案中,药物制剂的pH的飘移在±0.1范围内。
在一些实施方案中,如上任一项所述的药物组合物,其中所述特异性结合HGFR和EGFR的双特异性抗体的浓度为1mg/mL至250mg/mL。在一些实施方案中,所述特异性结合HGFR和EGFR的双特异性抗体的浓度为1mg/mL至200mg/mL。在一些实施方案中,所述特异性结合HGFR和EGFR的双特异性抗体的浓度为50mg/mL至200mg/mL。在一些实施方案中,所述特异性结合HGFR和EGFR的双特异性抗体的浓度为80mg/mL至180mg/mL。
在一些实施方案中,所述特异性结合HGFR和EGFR的双特异性抗体的浓度为1mg/mL至150mg/mL。在一些实施方案中,所述特异性结合HGFR和EGFR的双特异性抗体的浓度为1mg/mL至120mg/mL。在一些实施方案中,所述特异性结合HGFR和EGFR的双特异性抗体的浓度为1mg/mL至100mg/mL。在一些实施方
案中,所述特异性结合HGFR和EGFR的双特异性抗体的浓度为50mg/mL至150mg/mL。在一些实施方案中,所述特异性结合HGFR和EGFR的双特异性抗体的浓度为80mg/mL至120mg/mL。在一些实施方案中,所述特异性结合HGFR和EGFR的双特异性抗体的浓度为90mg/mL至110mg/mL。在一些实施方案中,所述特异性结合HGFR和EGFR的双特异性抗体的浓度为约100mg/mL。
在一些实施方案中,所述特异性结合HGFR和EGFR的双特异性抗体的浓度为80mg/mL至200mg/mL。在一些实施方案中,所述特异性结合HGFR和EGFR的双特异性抗体的浓度为90mg/mL至200mg/mL。在一些实施方案中,所述特异性结合HGFR和EGFR的双特异性抗体的浓度为100mg/mL至200mg/mL。在一些实施方案中,所述特异性结合HGFR和EGFR的双特异性抗体的浓度为120mg/mL至200mg/mL。在一些实施方案中,所述特异性结合HGFR和EGFR的双特异性抗体的浓度为120mg/mL至180mg/mL。在一些实施方案中,所述特异性结合HGFR和EGFR的双特异性抗体的浓度为135mg/mL至165mg/mL。在一些实施方案中,所述特异性结合HGFR和EGFR的双特异性抗体的浓度为约150mg/mL。
在一些实施方案中,所述特异性结合HGFR和EGFR的双特异性抗体的浓度为50mg/mL至100mg/mL。在一些实施方案中,所述特异性结合HGFR和EGFR的双特异性抗体的浓度为50mg/mL至75mg/mL。在一些实施方案中,所述特异性结合HGFR和EGFR的双特异性抗体的浓度为48mg/mL至72mg/mL。在一些实施方案中,所述特异性结合HGFR和EGFR的双特异性抗体的浓度为54mg/mL至66mg/mL。在一些实施方案中,所述特异性结合HGFR和EGFR的双特异性抗体的浓度为约60mg/mL。在一些实施方案中,所述特异性结合HGFR和EGFR的双特异性抗体的浓度为约70mg/mL。
在一些实施方案中,所述特异性结合HGFR和EGFR的双特异性抗体的浓度为50mg/mL至250mg/mL。在一些实施方案中,所述特异性结合HGFR和EGFR的双特异性抗体的浓度为100mg/mL至250mg/mL。在一些实施方案中,所述特异性结合HGFR和EGFR的双特异性抗体的浓度为160mg/mL至240mg/mL。在一些实施方案中,所述特异性结合HGFR和EGFR的双特异性抗体的浓度为180mg/mL至220mg/mL。在一些实施方案中,所述特异性结合HGFR和EGFR的双特异性抗体的浓度为约200mg/mL。
在一些实施方案中,所述特异性结合HGFR和EGFR的双特异性抗体的浓度为1mg/mL、5mg/mL、10mg/mL、20mg/mL、30mg/mL、40mg/mL、45mg/mL、48mg/mL、50mg/mL、54mg/mL、55mg/mL、60mg/mL、65mg/mL、66mg/mL、70mg/mL、72mg/mL、75mg/mL、80mg/mL、85mg/mL、90mg/mL、95mg/mL、100mg/mL、105mg/mL、110mg/mL、120mg/mL、130mg/mL、135mg/mL、140mg/mL、150mg/mL、160mg/mL、165mg/mL、180mg/mL、200mg/mL、220mg/mL、240mg/mL或250mg/mL,或者为这些点值之间的任意范围。在一些实施方案中,所述特异性
结合HGFR和EGFR的双特异性抗体的浓度为60mg/mL。在一些实施方案中,所述特异性结合HGFR和EGFR的双特异性抗体的浓度为70mg/mL。在一些实施方案中,所述特异性结合HGFR和EGFR的双特异性抗体的浓度为100mg/mL。在一些实施方案中,所述特异性结合HGFR和EGFR的双特异性抗体的浓度为150mg/mL。在一些实施方案中,所述特异性结合HGFR和EGFR的双特异性抗体的浓度为200mg/mL。
在一些实施方案中,如上任一项所述的药物组合物,其中所述药物组合物包含表面活性剂。在一些实施方案中,所述表面活性剂是非离子表面活性剂。在一些实施方案中,所述表面活性剂选自泊洛沙姆(例如泊洛沙姆188,简称P188)、聚山梨酯(例如聚山梨酯20、聚山梨酯80)、聚羟亚烃、Triton、十二烷基磺酸钠、月桂基磺酸钠、辛基糖甙钠、月桂基-磺基甜菜碱、肉豆蔻基-磺基甜菜碱、亚油基-磺基甜菜碱、硬脂基-磺基甜菜碱、月桂基-肌氨酸、肉豆蔻基-肌氨酸、亚油基-肌氨酸、硬脂基-肌氨酸、亚油基-甜菜碱、肉豆蔻基-甜菜碱、鲸蜡基-甜菜碱、月桂酰胺基丙基-甜菜碱、柯卡酰胺基丙基-甜菜碱、亚油酰胺基丙基-甜菜碱、肉豆蔻酰胺基丙基-甜菜碱、棕榈酰胺基丙基-甜菜碱、异硬脂酰胺基丙基-甜菜碱、肉豆蔻酰胺基丙基-二甲基胺、棕榈酰胺基丙基-二甲基胺、异硬脂酰胺基丙基-二甲基胺、甲基可可酰基钠、甲基油基牛磺酸钠、聚乙二醇、聚丙二醇、乙烯与丙烯二醇的共聚物等。在一些实施方案中,所述表面活性剂为聚山梨酯或泊洛沙姆。在一些实施方案中,所述表面活性剂为聚山梨酯80、聚山梨酯20或泊洛沙姆188。在一些实施方案中,所述表面活性剂为聚山梨酯80。
在一些实施方案中,如上任一项所述的药物组合物,其中所述表面活性剂浓度为0.01mg/mL至1.0mg/mL。在一些实施方案中,所述表面活性剂浓度为0.1mg/mL至0.8mg/mL。在一些实施方案中,所述表面活性剂浓度为0.2mg/mL至0.8mg/mL。在一些实施方案中,所述表面活性剂浓度为0.3mg/mL至0.8mg/mL。在一些实施方案中,所述表面活性剂浓度为0.4mg/mL至0.8mg/mL。在一些实施方案中,所述表面活性剂浓度为0.48mg/mL至0.72mg/mL。在一些实施方案中,所述表面活性剂浓度为0.54mg/mL至0.66mg/mL。在一些实施方案中,所述表面活性剂浓度为约0.6mg/mL。
在一些实施方案中,所述表面活性剂浓度为0.3mg/mL至0.6mg/mL。在一些实施方案中,所述表面活性剂浓度为0.4mg/mL至0.6mg/mL。在一些实施方案中,所述表面活性剂浓度为0.6mg/mL至0.8mg/mL。在一些实施方案中,所述表面活性剂浓度为0.2mg/mL至0.4mg/mL。在一些实施方案中,所述表面活性剂浓度为0.2mg/mL至0.3mg/mL。在一些实施方案中,所述表面活性剂浓度为0.24mg/mL至0.36mg/mL。在一些实施方案中,所述表面活性剂浓度为约0.24mg/mL。在一些实施方案中,所述表面活性剂浓度为约0.3mg/mL。
在一些实施方案中,所述表面活性剂浓度为0.01mg/mL、0.05mg/mL、0.1mg/mL、
0.15mg/mL、0.2mg/mL、0.24mg/mL、0.3mg/mL、0.36mg/mL、0.4mg/mL、0.5mg/mL、0.6mg/mL、0.7mg/mL、0.8mg/mL、0.9mg/mL或1.0mg/mL,或者为这些点值之间的任意范围。在一些实施方案中,所述表面活性剂浓度为0.6mg/mL。在一些实施方案中,所述表面活性剂浓度为0.24mg/mL。在一些实施方案中,所述表面活性剂浓度为0.3mg/mL。
在一些实施方案中,所述表面活性剂为0.6mg/mL的聚山梨酯80。在一些实施方案中,所述表面活性剂浓度为0.24mg/mL的聚山梨酯80。在一些实施方案中,所述表面活性剂浓度为0.3mg/mL的聚山梨酯80。
在一些实施方案中,如上任一项所述的药物组合物,其包含糖。在一些实施方案中,所述糖选自常规组合物(CH2O)n及其衍生物,包括单糖,二糖,三糖,多糖,糖醇,还原性糖,非还原性糖等等。所述的糖可选自蔗糖,海藻糖,葡萄糖,乳糖,果糖,麦芽糖,右旋糖苷,甘油,赤藻糖醇,丙三醇,***糖醇,sylitol,山梨糖醇,甘露醇,密二糖,松三糖,蜜三糖,甘露三糖,水苏糖,麦芽糖,乳果糖,麦芽酮糖,山梨醇,麦芽糖醇,乳糖醇,异-麦芽酮糖等。
在一些实施方案中,所述糖为蔗糖、海藻糖、甘露醇或山梨糖醇。在一些实施方案中,所述糖为蔗糖或海藻糖。在一些实施方案中,所述糖为蔗糖。
在一些实施方案中,所述糖的浓度为10mg/mL至100mg/mL。在一些实施方案中,所述糖的浓度为20mg/mL至90mg/mL。在一些实施方案中,所述糖的浓度为20mg/mL至80mg/mL。在一些实施方案中,所述糖的浓度为30mg/mL至80mg/mL。在一些实施方案中,所述糖的浓度为50mg/mL至80mg/mL。在一些实施方案中,所述糖的浓度为60mg/mL至90mg/mL。在一些实施方案中,所述糖的浓度为60mg/mL至80mg/mL。在一些实施方案中,所述糖的浓度为67.5mg/mL至82.5mg/mL。在一些实施方案中,所述糖的浓度为70mg/mL至80mg/mL。在一些实施方案中,所述糖的浓度为约75mg/mL。
在一些实施方案中,所述糖的浓度为56mg/mL至84mg/mL。在一些实施方案中,所述糖的浓度为63mg/mL至77mg/mL。在一些实施方案中,所述糖的浓度为约70mg/mL。
在一些实施方案中,所述糖的浓度为20mg/mL至50mg/mL。在一些实施方案中,所述糖的浓度为20mg/mL至40mg/mL。在一些实施方案中,所述糖的浓度为20mg/mL至35mg/mL。在一些实施方案中,所述糖的浓度为22.4mg/mL至33.6mg/mL。在一些实施方案中,所述糖的浓度为25.2mg/mL至30.8mg/mL。在一些实施方案中,所述糖的浓度为约28mg/mL。
在一些实施方案中,所述糖的浓度为30mg/mL至50mg/mL。在一些实施方案中,所述糖的浓度为30mg/mL至40mg/mL。在一些实施方案中,所述糖的浓度为约37.5mg/mL。
在一些实施方案中,所述糖的浓度非限制性实施包括10mg/mL、20mg/mL、
22.4mg/mL、25.2mg/mL、28mg/mL、30mg/mL、30.8mg/mL、33.6mg/mL、35mg/mL、37.5mg/mL、40mg/mL、45mg/mL、50mg/mL、56mg/mL、55mg/mL、60mg/mL、63mg/mL、65mg/mL、67.5mg/mL、70mg/mL、75mg/mL、77mg/mL、80mg/mL、82.5mg/mL、84mg/mL、85mg/mL、90mg/mL、95mg/mL、100mg/mL,以及这些点值之间的任一范围。在一些实施方案中,所述糖的浓度为28mg/mL。在一些实施方案中,所述糖的浓度为37.5mg/mL。在一些实施方案中,所述糖的浓度为70mg/mL。在一些实施方案中,所述糖的浓度为75mg/mL。
在一些实施方案中,所述糖为70mg/mL至80mg/mL的蔗糖;优选地,所述糖为75mg/mL的蔗糖。
在一些实施方案中,所述糖为63mg/mL至77mg/mL的蔗糖;优选地,所述糖为70mg/mL的蔗糖。
在一些实施方案中,所述糖为25.2mg/mL至30.8mg/mL的蔗糖;优选地,所述糖为28mg/mL的蔗糖。
在一些实施方案中,如上任一项所述的药物组合物,所述缓冲剂的浓度为5mM至100mM。在一些实施方案中,所述缓冲剂的浓度为5mM至50mM。在一些实施方案中,所述缓冲剂的浓度为10mM至50mM。在一些实施方案中,所述缓冲剂的浓度为5mM至30mM。在一些实施方案中,所述缓冲剂的浓度为10mM至30mM。在一些实施方案中,所述缓冲剂的浓度为15mM至25mM。在一些实施方案中,所述缓冲剂的浓度为18mM至22mM。在一些实施方案中,所述缓冲剂的浓度为10mM至20mM。在一些实施方案中,所述缓冲剂的浓度为10mM或20mM。在一些实施方案中,所述缓冲剂的浓度为约20mM。
在一些实施方案中,所述缓冲剂的浓度为20mM至30mM。在一些实施方案中,所述缓冲剂的浓度为22.5mM至27.5mM。在一些实施方案中,所述缓冲剂的浓度为约25mM。
在一些实施方案中,所述缓冲剂的浓度为5mM至15mM。在一些实施方案中,所述缓冲剂的浓度为8mM至12mM。在一些实施方案中,所述缓冲剂的浓度为9mM至11mM。在一些实施方案中,所述缓冲剂的浓度为约10mM。
在一些实施方案中,所述缓冲剂的浓度为5mM、8mM、9mM、10mM、11mM、12mM、15mM、18mM、20mM、22mM、22.5mM、25mM、27.5mM、30mM、40mM、50mM、60mM、70mM、80mM、90mM或100mM,以及这些点值之间的任一范围。在一些实施方案中,所述缓冲剂的浓度为10mM。在一些实施方案中,所述缓冲剂的浓度为20mM。在一些实施方案中,所述缓冲剂的浓度为25mM。
在一些实施方案中,所述缓冲剂为10mM或20mM的组氨酸-盐酸组氨酸缓冲剂。在一些实施方案中,所述缓冲剂为10mM或20mM组氨酸-醋酸组氨酸缓冲剂。在一些实施方案中,所述缓冲剂为25mM组氨酸-盐酸组氨酸缓冲剂。
在一些实施方案中,如上任一项所述的药物组合物,其中所述药物组合物还
包含辅料。在一些实施方案中,所述辅料为甲硫氨酸、盐酸精氨酸、甘氨酸、脯氨酸、组氨酸、苯丙氨酸、谷氨酸、天冬氨酸、氯化钠、氯化钙或乙二胺四乙酸二钠。在一些实施方案中,所述辅料为氯化钠或氯化钙。在一些实施方案中,所述辅料为甲硫氨酸或盐酸精氨酸。在一些实施方案中,所述辅料为盐酸精氨酸。在一些实施方案中,所述辅料为甲硫氨酸。
在一些实施方案中,如上任一项所述的药物组合物,其中所述辅料的浓度为1mM至300mM;优选地,辅料的浓度为25mM至200mM。在一些实施方案中,所述辅料的浓度为1mM至200mM。在一些实施方案中,所述辅料的浓度为1mM至20mM。在一些实施方案中,所述辅料的浓度为4mM至20mM。在一些实施方案中,所述辅料的浓度为8mM至12mM。在一些实施方案中,所述辅料的浓度为9mM至11mM。在一些实施方案中,所述辅料的浓度为约10mM。
在一些实施方案中,所述辅料的浓度为1mM至10mM。在一些实施方案中,所述辅料的浓度为3mM至5mM。在一些实施方案中,所述辅料的浓度为3.6mM至4.4mM。在一些实施方案中,所述辅料的浓度为约4mM。
在一些实施方案中,所述辅料的浓度为1mM至240mM。在一些实施方案中,所述辅料的浓度为80mM至240mM。在一些实施方案中,所述辅料的浓度为160mM至240mM。在一些实施方案中,所述辅料的浓度为180mM至220mM。在一些实施方案中,所述辅料的浓度为约200mM。
在一些实施方案中,所述辅料的浓度为80mM至120mM。在一些实施方案中,所述辅料的浓度为90mM至110mM。在一些实施方案中,所述辅料的浓度为约100mM。
在一些实施方案中,辅料的浓度为1mM、3mM、3.6mM、4.4mM、5mM、8mM、9mM、10mM、11mM、12mM、20mM、25mM、30mM、50mM、75mM、80mM、90mM、100mM、110mM、120mM、150mM、160mM、180mM、200mM、240mM、250mM、280mM或300mM,以及这些点值之间的任一范围。在一些实施方案中,所述辅料的浓度为4mM。在一些实施方案中,所述辅料的浓度为10mM。在一些实施方案中,如上任一项所述的药物组合物,其中所述辅料的浓度为100mM。在一些实施方案中,如上任一项所述的药物组合物,其中所述辅料的浓度为200mM。
在一些实施方案中,如上任一项所述的药物组合物,其中所述辅料的浓度为4mM或10mM的甲硫氨酸。
在一些实施方案中,如上任一项所述的药物组合物,其中所述辅料的浓度为100mM或200mM的盐酸精氨酸。
在一些实施方案中,如上任一项所述的药物组合物,其中所述药物组合物还包含酶。在一些实施方案中,所述酶为透明质酸酶(PH20)。
在一些实施方案中,如上任一项所述的药物组合物,其中所述酶的浓度为10
U/mL至3000U/mL。在一些实施方案中,如上任一项所述的药物组合物,其中所述酶的浓度为150U/mL至2400U/mL。在一些实施方案中,如上任一项所述的药物组合物,其中所述酶的浓度为150U/mL至2200U/mL。在一些实施方案中,如上任一项所述的药物组合物,其中所述酶的浓度为600U/mL至2200U/mL。在一些实施方案中,如上任一项所述的药物组合物,其中所述酶的浓度为800U/mL至2000U/mL。
在一些实施方案中,如上任一项所述的药物组合物,其中所述酶的浓度为700U/mL至2400U/mL。在一些实施方案中,如上任一项所述的药物组合物,其中所述酶的浓度为800U/mL至2400U/mL。在一些实施方案中,如上任一项所述的药物组合物,其中所述酶的浓度为1600U/mL至2400U/mL。在一些实施方案中,如上任一项所述的药物组合物,其中所述酶的浓度为1800U/mL至2200U/mL。在一些实施方案中,如上任一项所述的药物组合物,其中所述酶的浓度为约2000U/mL。
在一些实施方案中,如上任一项所述的药物组合物,其中所述酶的浓度为150U/mL至2000U/mL。在一些实施方案中,如上任一项所述的药物组合物,其中所述酶的浓度为500U/mL至1100U/mL。在一些实施方案中,如上任一项所述的药物组合物,其中所述酶的浓度为600U/mL至1000U/mL。在一些实施方案中,如上任一项所述的药物组合物,其中所述酶的浓度为640U/mL至960U/mL。在一些实施方案中,如上任一项所述的药物组合物,其中所述酶的浓度为700U/mL至900U/mL。在一些实施方案中,如上任一项所述的药物组合物,其中所述酶的浓度为720U/mL至880U/mL。在一些实施方案中,如上任一项所述的药物组合物,其中所述酶的浓度为约800U/mL。
在一些实施方案中,所述酶的浓度为10U/mL、50U/mL、100U/mL、150U/mL、300U/mL、500U/mL、600U/mL、640U/mL、700U/mL、720U/mL、800U/mL、880U/mL、900U/mL、960U/mL、1000U/mL、1100U/mL、1200U/mL、1500U/mL、1600U/mL、1800U/mL、2000U/mL、2200U/mL、2400U/mL、2500U/mL、2800U/mL或3000U/mL,以及这些点值之间的任一范围。在一些实施方案中,如上任一项所述的药物组合物,其中所述酶的浓度为800U/mL。在一些实施方案中,如上任一项所述的药物组合物,其中所述酶的浓度为1000U/mL。在一些实施方案中,如上任一项所述的药物组合物,其中所述酶的浓度为2000U/mL。
在一些实施方案中,如上任一项所述的药物组合物,其中所述酶为1000U/mL或2000U/mL的PH20。在一些实施方案中,如上任一项所述的药物组合物,其中所述酶为800U/mL的PH20。
在一些实施方案中,如上任一项所述的药物组合物,其中所述的特异性结合HGFR和EGFR的双特异性抗体中,
所述抗原结合模块1包含重链可变区M-VH和轻链可变区M-VL,所述M-VH
包含M-HCDR1、M-HCDR2和M-HCDR3,所述M-VL包含M-LCDR1、M-LCDR2和M-LCDR3,其中:
所述E-HCDR1、E-HCDR2、E-HCDR3、E-LCDR1、E-LCDR2和E-LCDR3是根据Kabat编号规则定义的,所述E-HCDR1如SEQ ID NO:6所示,所述E-HCDR2如SEQ ID NO:7所示,所述E-HCDR3如SEQ ID NO:8所示,所述E-LCDR1如SEQ ID NO:9所示,所述E-LCDR2如SEQ ID NO:10所示,和所述E-LCDR3如SEQ ID NO:11所示;和
所述M-HCDR1、M-HCDR2、M-HCDR3、M-LCDR1、M-LCDR2和M-LCDR3是根据Kabat编号规则定义的,其中,
(i)所述M-HCDR1如SEQ ID NO:30所示,所述M-HCDR2如SEQ ID NO:31所示,所述M-HCDR3如SEQ ID NO:32所示,所述M-LCDR1如SEQ ID NO:27所示,所述M-LCDR2如SEQ ID NO:33所示,和所述M-LCDR3如SEQ ID NO:29所示,或
(ii)所述M-HCDR1如SEQ ID NO:18所示,所述M-HCDR2包含SEQ ID NO:19所示,所述M-HCDR3包含SEQ ID NO:20所示,所述M-LCDR1包含SEQ ID NO:21所示,所述M-LCDR2包含SEQ ID NO:22所示,和所述M-LCDR3包含SEQ ID NO:23所示,或
(iii)所述M-HCDR1如SEQ ID NO:24所示,所述M-HCDR2如SEQ ID NO:25所示,所述M-HCDR3如SEQ ID NO:26所示,所述M-LCDR1如SEQ ID NO:27所示,所述M-LCDR2如SEQ ID NO:28所示,和所述M-LCDR3如SEQ ID NO:29所示;
优选地,
(i)所述特异性结合HGFR和EGFR的双特异性抗体包含两个特异性结合HGFR的抗原结合模块1和一个特异性结合EGFR的抗原结合模块2,
其中一个抗原结合模块1,其M-VH具有:如SEQ ID NO:30所示的M-HCDR1、如SEQ ID NO:31所示的M-HCDR2和如SEQ ID NO:32所示的M-HCDR3,并且其M-VL具有:如SEQ ID NO:27所示的M-LCDR1、如SEQ ID NO:33所示的M-LCDR2和如SEQ ID NO:29所示的M-LCDR3;
另一个抗原结合模块1,其M-VH具有:如SEQ ID NO:18所示的M-HCDR1、如SEQ ID NO:19所示的M-HCDR2和如SEQ ID NO:20所示的M-HCDR3,并且其M-VL具有:如SEQ ID NO:21所示的M-LCDR1、如SEQ ID NO:22所示的M-LCDR2和如SEQ ID NO:23所示的M-LCDR3;和
所述抗原结合模块2,其E-VH具有:如SEQ ID NO:6所示的E-HCDR1、如SEQ ID NO:7所示的E-HCDR2和如SEQ ID NO:8所示的E-HCDR3,并且其E-VL具有:如SEQ ID NO:9所示的E-LCDR1、如SEQ ID NO:10所示的E-LCDR2和如SEQ ID NO:11所示的E-LCDR3;或
(ii)所述特异性结合HGFR和EGFR的双特异性抗体包含一个特异性结合HGFR的抗原结合模块1和一个特异性结合EGFR的抗原结合模块2,
所述抗原结合模块1,其M-VH具有:如SEQ ID NO:18所示的M-HCDR1、如SEQ ID NO:19所示的M-HCDR2和如SEQ ID NO:20所示的M-HCDR3,并且其M-VL具有:如SEQ ID NO:21所示的M-LCDR1、如SEQ ID NO:22所示的M-LCDR2和如SEQ ID NO:23所示的M-LCDR3;和
所述抗原结合模块2,其E-VH具有:如SEQ ID NO:6所示的E-HCDR1、如SEQ ID NO:7所示的E-HCDR2和如SEQ ID NO:8所示的E-HCDR3,并且其E-VL具有:如SEQ ID NO:9所示的E-LCDR1、如SEQ ID NO:10所示的E-LCDR2和如SEQ ID NO:11所示的E-LCDR3。
在一些实施方案中,如上任一项所述的药物组合物,其中所述的特异性结合HGFR和EGFR的双特异性抗体,其中:
(i)所述M-VH包含与SEQ ID NO:16具有至少90%的序列同一性的氨基酸序列,并且所述M-VL包含与SEQ ID NO:17具有至少90%的序列同一性的氨基酸序列;或
所述M-VH包含与SEQ ID NO:12具有至少90%的序列同一性的氨基酸序列,并且所述M-VL包含与SEQ ID NO:13具有至少90%的序列同一性的氨基酸序列;或
所述M-VH包含与SEQ ID NO:14具有至少90%的序列同一性的氨基酸序列,并且所述M-VL包含与SEQ ID NO:15具有至少90%的序列同一性的氨基酸序列;和/或
(ii)所述E-VH包含与SEQ ID NO:3具有至少90%的序列同一性的氨基酸序列,并且所述E-VL包含与SEQ ID NO:5或SEQ ID NO:4具有至少90%的序列同一性的氨基酸序列;
优选地,
(i)所述特异性结合HGFR和EGFR的双特异性抗体包含两个特异性结合HGFR的抗原结合模块1和一个特异性结合EGFR的抗原结合模块2,
其中一个抗原结合模块1的M-VH如SEQ ID NO:16所示,并且M-VL如SEQ ID NO:17所示;
另一个抗原结合模块1的M-VH如SEQ ID NO:12所示,并且M-VL如SEQ ID NO:13所示;和
所述抗原结合模块2的E-VH如SEQ ID NO:3所示,并且E-VL如SEQ ID NO:5所示;或
(ii)所述特异性结合HGFR和EGFR的双特异性抗体包含一个特异性结合HGFR的抗原结合模块1和一个特异性结合EGFR的抗原结合模块2,
所述抗原结合模块1的M-VH如SEQ ID NO:12所示,和所述M-VL如SEQ
ID NO:13所示,并且所述抗原结合模块2的E-VH如SEQ ID NO:3所示,和E-VL如SEQ ID NO:5所示。
在一些实施方案中,如上任一项所述的药物组合物,其中:
所述的特异性结合HGFR和EGFR的双特异性抗体,其具有一条如SEQ ID NO:34所示的第一链、一条包含SEQ ID NO:35所示的第二链、一条包含SEQ ID NO:36所示的第三链和一条包含SEQ ID NO:37所示的第四链;或
具有一条如SEQ ID NO:38所示的第一链、一条如SEQ ID NO:39所示的第二链、一条如SEQ ID NO:40所示的第三链和一条如SEQ ID NO:41所示的第四链。
在一些实施方案中,如上任一项所述的药物组合物,其中所述的特异性结合HGFR和EGFR的双特异性抗体具有Format1或Format2结构,Format1的结构示意图见图1,Format2的结构示意图见图2。
在一些实施方式中,如上任一项所述的药物组合物,其中所述的特异性结合HGFR和EGFR的双特异性抗体,其为低岩藻糖基化双特异性抗体;优选地,所述低岩藻糖基化双特异性抗体为至少80%、85%、90%、95%或100%未被岩藻糖糖基化修饰的抗体;更优选地,所述双特异性抗体检测不到岩藻糖糖基化修饰。
在一些实施方案中,如上任一项所述的药物组合物,其包含如下组分:
(a)1mg/mL至250mg/mL的所述特异性结合HGFR和EGFR的双特异性抗体,
(b)0.01mg/mL至1.0mg/mL的表面活性剂,
(c)10mg/mL至100mg/mL的糖,和
(d)5mM至100mM的缓冲剂,所述药物组合物的pH为5.0至6.5。
在一些实施方案中,如上任一项所述的药物组合物,其包含如下组分:
(a)1mg/mL至200mg/mL的所述特异性结合HGFR和EGFR的双特异性抗体,
(b)0.1mg/mL至0.8mg/mL的聚山梨酯80,
(c)30mg/mL至80mg/mL的蔗糖,和
(d)10mM至50mM的组氨酸缓冲剂;所述药物组合物的pH为5.5至6.5。
在一些实施方案中,如上任一项所述的药物组合物,其包含如下组分:
(a)90mg/mL至110mg/mL的特异性结合HGFR和EGFR的双特异性抗体,
(b)0.4mg/mL至0.8mg/mL的聚山梨酯80,
(c)70mg/mL至80mg/mL的蔗糖,和
(d)10mM至20mM的组氨酸-盐酸组氨酸缓冲剂或组氨酸-醋酸组氨酸缓冲剂;所述药物组合物的pH为约6.0。
在一些实施方案中,如上任一项所述的药物组合物,其包含如下组分:
(a)90mg/mL至110mg/mL的特异性结合HGFR和EGFR的双特异性抗体,
(b)0.6mg/mL的聚山梨酯80,
(c)70mg/mL至80mg/mL的蔗糖,和
(d)20mM的组氨酸-盐酸组氨酸缓冲剂;所述药物组合物的pH为约6.0。
在一些实施方案中,如上任一项所述的药物组合物,其包含如下组分:
(a)120mg/mL的特异性结合HGFR和EGFR的双特异性抗体,
(b)0.6mg/mL的聚山梨酯80,
(c)70mg/mL至80mg/mL的蔗糖,
(d)2000U/mL的PH20,和
(e)20mM的组氨酸-盐酸组氨酸缓冲剂;所述药物组合物的pH为约6.0。
在一些实施方案中,如上任一项所述的药物组合物,其包含如下组分:
(a)150mg/mL的特异性结合HGFR和EGFR的双特异性抗体,
(b)0.6mg/mL的聚山梨酯80,
(c)70mg/mL至80mg/mL的蔗糖,
(d)2000U/mL的PH20,和
(e)20mM的组氨酸-盐酸组氨酸缓冲剂;所述药物组合物的pH为约5.0。
在一些实施方案中,如上任一项所述的药物组合物,其包含如下组分:
(a)200mg/mL的特异性结合HGFR和EGFR的双特异性抗体,
(b)0.6mg/mL的聚山梨酯80,
(c)70mg/mL至80mg/mL的蔗糖,
(d)200mM的盐酸精氨酸,
(e)2000U/mL的PH20,和
(f)20mM的组氨酸-盐酸组氨酸缓冲剂;所述药物组合物的pH为约5.0。
在一些实施方案中,如上任一项所述的药物组合物,其包含如下组分:
(a)200mg/mL的特异性结合HGFR和EGFR的双特异性抗体,
(b)0.6mg/mL的聚山梨酯80,
(c)70mg/mL至80mg/mL的蔗糖,
(d)100mM的盐酸精氨酸,
(e)2000U/mL的PH20,和
(f)20mM的组氨酸-盐酸组氨酸缓冲剂;所述药物组合物的pH为约5.0。
在一些实施方案中,如上任一项所述的药物组合物,其包含如下组分:
(a)1mg/mL至250mg/mL的所述特异性结合HGFR和EGFR的双特异性抗体,
(b)0.01mg/mL至1.0mg/mL的表面活性剂,
(c)10mg/mL至100mg/mL的糖,和
(d)5mM至100mM的缓冲剂,所述药物组合物的pH为4.5至6.5。
在一些实施方案中,如上任一项所述的药物组合物,其包含如下组分:
(a)1mg/mL至200mg/mL的所述特异性结合HGFR和EGFR的双特异性抗体,
(b)0.1mg/mL至0.8mg/mL的聚山梨酯80,
(c)20mg/mL至80mg/mL的蔗糖,和
(d)5mM至50mM的组氨酸缓冲剂;所述药物组合物的pH为4.8至6.2。
在一些实施方案中,如上任一项所述的药物组合物,其包含如下组分:
(a)80mg/mL至180mg/mL的特异性结合HGFR和EGFR的双特异性抗体,
(b)0.4mg/mL至0.8mg/mL的聚山梨酯80,
(c)60mg/mL至80mg/mL的蔗糖,和
(d)5mM至30mM的组氨酸-盐酸组氨酸缓冲剂或组氨酸-醋酸组氨酸缓冲剂;所述药物组合物的pH为5.0至6.0。
在一些实施方案中,如上任一项所述的药物组合物,其包含如下组分:
(a)1mg/mL至200mg/mL的所述特异性结合HGFR和EGFR的双特异性抗体,
(b)0.1mg/mL至0.8mg/mL的聚山梨酯80,
(c)20mg/mL至80mg/mL的蔗糖,和
(d)5mM至50mM的组氨酸缓冲剂;所述药物组合物的pH为5.0至6.5。
在一些实施方案中,如上任一项所述的药物组合物,其包含如下组分:
(a)50mg/mL至200mg/mL的特异性结合HGFR和EGFR的双特异性抗体,
(b)0.4mg/mL至0.8mg/mL的聚山梨酯80,
(c)60mg/mL至80mg/mL的蔗糖,和
(d)10mM至30mM的组氨酸-盐酸组氨酸缓冲剂或组氨酸-醋酸组氨酸缓冲剂;所述药物组合物的pH为5.5至6.5。
在一些实施方案中,如上任一项所述的药物组合物,其包含如下组分:
(a)90mg/mL至110mg/mL的特异性结合HGFR和EGFR的双特异性抗体,
(b)0.4mg/mL至0.8mg/mL的聚山梨酯80,
(c)70mg/mL至80mg/mL的蔗糖,和
(d)15mM至25mM的组氨酸-盐酸组氨酸缓冲剂;所述药物组合物的pH为5.8至6.2。
在一些实施方案中,如上任一项所述的药物组合物,其包含如下组分:
(a)约100mg/mL的特异性结合HGFR和EGFR的双特异性抗体,
(b)约0.6mg/mL的聚山梨酯80,
(c)约75mg/mL的蔗糖,和
(d)约20mM的组氨酸-盐酸组氨酸缓冲剂;所述药物组合物的pH为约6.0。
在一些实施方案中,如上任一项所述的药物组合物,其包含如下组分:
(a)100mg/mL的特异性结合HGFR和EGFR的双特异性抗体,
(b)0.6mg/mL的聚山梨酯80,
(c)75mg/mL的蔗糖,和
(d)20mM的组氨酸-盐酸组氨酸缓冲剂;所述药物组合物的pH为6.0。
在一些实施方案中,如上任一项所述的药物组合物,其包含如下组分:
(a)1mg/mL至250mg/mL的所述特异性结合HGFR和EGFR的双特异性抗体,
(b)0.01mg/mL至1.0mg/mL的表面活性剂,
(c)10mg/mL至100mg/mL的糖,
(d)1mM至300mM的辅料,
(e)10U/mL至3000U/mL的酶,和
(f)5mM至100mM的缓冲剂,所述药物组合物的pH为4.5至6.5。
在一些实施方案中,如上任一项所述的药物组合物,其包含如下组分:
(a)1mg/mL至200mg/mL的所述特异性结合HGFR和EGFR的双特异性抗体,
(b)0.1mg/mL至0.8mg/mL的聚山梨酯80,
(c)20mg/mL至80mg/mL的蔗糖,
(d)1mM至200mM的甲硫氨酸,
(e)700U/mL至2400U/mL的透明质酸酶,和
(f)5mM至50mM的组氨酸缓冲剂;所述药物组合物的pH为4.8至6.2。
在一些实施方案中,如上任一项所述的药物组合物,其包含如下组分:
(a)80mg/mL至180mg/mL的特异性结合HGFR和EGFR的双特异性抗体,
(b)0.4mg/mL至0.8mg/mL的聚山梨酯80,
(c)60mg/mL至80mg/mL的蔗糖,
(d)1mM至20mM的甲硫氨酸,
(e)1600U/mL至2400U/mL的透明质酸酶,和
(f)5mM至30mM的组氨酸-盐酸组氨酸缓冲剂或组氨酸-醋酸组氨酸缓冲剂;所述药物组合物的pH为5.0至6.0。
在一些实施方案中,如上任一项所述的药物组合物,其包含如下组分:
(a)50mg/mL至200mg/mL的所述特异性结合HGFR和EGFR的双特异性抗体,
(b)0.1mg/mL至0.8mg/mL的聚山梨酯80,
(c)20mg/mL至80mg/mL的蔗糖,
(d)1mM至200mM的甲硫氨酸,
(e)150U/mL至2200U/mL的透明质酸酶,和
(f)5mM至50mM的组氨酸缓冲剂;所述药物组合物的pH为4.5至6.0。
在一些实施方案中,如上任一项所述的药物组合物,其包含如下组分:
(a)100mg/mL至200mg/mL的特异性结合HGFR和EGFR的双特异性抗体,
(b)0.4mg/mL至0.8mg/mL的聚山梨酯80,
(c)60mg/mL至80mg/mL的蔗糖,
(d)4mM至20mM的甲硫氨酸,
(e)800U/mL至2200U/mL的透明质酸酶,和
(f)10mM至30mM的组氨酸-盐酸组氨酸缓冲剂或组氨酸-醋酸组氨酸缓冲剂;所述药物组合物的pH为4.5至5.5。
在一些实施方案中,如上任一项所述的药物组合物,其包含如下组分:
(a)120mg/mL至180mg/mL的特异性结合HGFR和EGFR的双特异性抗体,
(b)0.4mg/mL至0.8mg/mL的聚山梨酯80,
(c)60mg/mL至80mg/mL的蔗糖,
(d)1mM至20mM的甲硫氨酸,
(e)1600U/mL至2400U/mL的透明质酸酶,和
(f)20mM至30mM的组氨酸-盐酸组氨酸缓冲剂;所述药物组合物的pH为4.8至5.2。
在一些实施方案中,如上任一项所述的药物组合物,其包含如下组分:
(a)约150mg/mL的特异性结合HGFR和EGFR的双特异性抗体,
(b)约0.6mg/mL的聚山梨酯80,
(c)约70mg/mL的蔗糖,
(d)约10mM的甲硫氨酸,
(e)约2000U/mL的透明质酸酶,和
(f)约25mM的组氨酸-盐酸组氨酸缓冲剂;所述药物组合物的pH为约5.0。
在一些实施方案中,如上任一项所述的药物组合物,其包含如下组分:
(a)150mg/mL的特异性结合HGFR和EGFR的双特异性抗体,
(b)0.6mg/mL的聚山梨酯80,
(c)70mg/mL的蔗糖,
(d)10mM的甲硫氨酸,
(e)2000U/mL的透明质酸酶,和
(f)25mM的组氨酸-盐酸组氨酸缓冲剂;所述药物组合物的pH为5.0。
在一些实施方案中,如上任一项所述的药物组合物,其包含如下组分:
(a)50mg/mL至100mg/mL的特异性结合HGFR和EGFR的双特异性抗体,
(b)0.2mg/mL至0.4mg/mL的聚山梨酯80,
(c)20mg/mL至50mg/mL的蔗糖,
(d)1mM至10mM的甲硫氨酸,
(e)600U/mL至1000U/mL的透明质酸酶,和
(f)5mM至15mM的组氨酸-盐酸组氨酸缓冲剂或组氨酸-醋酸组氨酸缓冲剂;
所述药物组合物的pH为4.5至5.5。
在一些实施方案中,如上任一项所述的药物组合物,其包含如下组分:
(a)50mg/mL至75mg/mL的特异性结合HGFR和EGFR的双特异性抗体,
(b)0.2mg/mL至0.3mg/mL的聚山梨酯80,
(c)20mg/mL至35mg/mL的蔗糖,
(d)1mM至10mM的甲硫氨酸,
(e)720U/mL至880U/mL的透明质酸酶,和
(f)8mM至12mM的组氨酸-盐酸组氨酸缓冲剂;所述药物组合物的pH为4.8至5.2。
在一些实施方案中,如上任一项所述的药物组合物,其包含如下组分:
(a)约60mg/mL的特异性结合HGFR和EGFR的双特异性抗体,
(b)约0.24mg/mL的聚山梨酯80,
(c)约28mg/mL的蔗糖,
(d)约4mM的甲硫氨酸,
(e)约800U/mL的透明质酸酶,和
(f)约10mM的组氨酸-盐酸组氨酸缓冲剂;所述药物组合物的pH为约5.0。
在一些实施方案中,如上任一项所述的药物组合物,其包含如下组分:
(a)60mg/mL的特异性结合HGFR和EGFR的双特异性抗体,
(b)0.24mg/mL的聚山梨酯80,
(c)28mg/mL的蔗糖,
(d)4mM的甲硫氨酸,
(e)800U/mL的透明质酸酶,和
(f)10mM的组氨酸-盐酸组氨酸缓冲剂;所述药物组合物的pH为5.0。
在一些实施方案中,如上任一项所述的药物组合物,其包含如下组分:
(a)50mg/mL至250mg/mL的所述特异性结合HGFR和EGFR的双特异性抗体,
(b)0.1mg/mL至0.8mg/mL的聚山梨酯80,
(c)20mg/mL至80mg/mL的蔗糖,
(d)80mM至240mM的盐酸精氨酸,和
(e)5mM至50mM的组氨酸缓冲剂;所述药物组合物的pH为4.5至6.0。
在一些实施方案中,如上任一项所述的药物组合物,其包含如下组分:
(a)160mg/mL至240mg/mL的特异性结合HGFR和EGFR的双特异性抗体,
(b)0.4mg/mL至0.8mg/mL的聚山梨酯80,
(c)60mg/mL至80mg/mL的蔗糖,
(d)160mM至240mM的盐酸精氨酸,和
(e)10mM至30mM的组氨酸-盐酸组氨酸缓冲剂或组氨酸-醋酸组氨酸缓冲
剂;所述药物组合物的pH为4.5至5.5。
在一些实施方案中,如上任一项所述的药物组合物,其包含如下组分:
(a)180mg/mL至220mg/mL的特异性结合HGFR和EGFR的双特异性抗体,
(b)0.4mg/mL至0.8mg/mL的聚山梨酯80,
(c)70mg/mL至80mg/mL的蔗糖,
(d)180mM至220mM的盐酸精氨酸,和
(e)15mM至25mM的组氨酸-盐酸组氨酸缓冲剂;所述药物组合物的pH为4.8至5.2。
在一些实施方案中,如上任一项所述的药物组合物,其包含如下组分:
(a)约200mg/mL的特异性结合HGFR和EGFR的双特异性抗体,
(b)约0.6mg/mL的聚山梨酯80,
(c)约75mg/mL的蔗糖,
(d)约200mM的盐酸精氨酸,和
(e)约20mM的组氨酸-盐酸组氨酸缓冲剂;所述药物组合物的pH为约5.0。
在一些实施方案中,如上任一项所述的药物组合物,其包含如下组分:
(a)200mg/mL的特异性结合HGFR和EGFR的双特异性抗体,
(b)0.6mg/mL的聚山梨酯80,
(c)75mg/mL的蔗糖,
(d)200mM的盐酸精氨酸,和
(e)20mM的组氨酸-盐酸组氨酸缓冲剂;所述药物组合物的pH为5.0。
在一些实施方案中,如上任一项所述的药物组合物,其包含如下组分:
(a)50mg/mL至150mg/mL的特异性结合HGFR和EGFR的双特异性抗体,
(b)0.2mg/mL至0.4mg/mL的聚山梨酯80,
(c)20mg/mL至50mg/mL的蔗糖,
(d)80mM至120mM的盐酸精氨酸,和
(e)5mM至15mM的组氨酸-盐酸组氨酸缓冲剂或组氨酸-醋酸组氨酸缓冲剂;所述药物组合物的pH为4.5至5.5。
在一些实施方案中,如上任一项所述的药物组合物,其包含如下组分:
(a)80mg/mL至120mg/mL的特异性结合HGFR和EGFR的双特异性抗体,
(b)0.2mg/mL至0.4mg/mL的聚山梨酯80,
(c)30mg/mL至50mg/mL的蔗糖,
(d)90mM至110mM的盐酸精氨酸,和
(e)15mM至25mM的组氨酸-盐酸组氨酸缓冲剂;所述药物组合物的pH为4.8至5.2。
在一些实施方案中,如上任一项所述的药物组合物,其包含如下组分:
(a)约100mg/mL的特异性结合HGFR和EGFR的双特异性抗体,
(b)约0.3mg/mL的聚山梨酯80,
(c)约37.5mg/mL的蔗糖,
(d)约100mM的盐酸精氨酸,和
(e)约10mM的组氨酸-盐酸组氨酸缓冲剂;所述药物组合物的pH为约5.0。
在一些实施方案中,如上任一项所述的药物组合物,其包含如下组分:
(a)100mg/mL的特异性结合HGFR和EGFR的双特异性抗体,
(b)0.3mg/mL的聚山梨酯80,
(c)37.5mg/mL的蔗糖,
(d)100mM的盐酸精氨酸,和
(e)10mM的组氨酸-盐酸组氨酸缓冲剂;所述药物组合物的pH为5.0。
在一些实施方案中,如上任一项所述的药物组合物,其包含如下组分:
(a)120mg/mL的特异性结合HGFR和EGFR的双特异性抗体,
(b)0.6mg/mL的聚山梨酯80,
(c)75mg/mL的蔗糖,
(d)10mM的甲硫氨酸,
(e)2000U/mL的PH20,和
(f)20mM的组氨酸-盐酸组氨酸缓冲剂;所述药物组合物的pH为5.0或6.0。
在一些实施方案中,如上任一项所述的药物组合物,其包含如下组分:
(a)120mg/mL的特异性结合HGFR和EGFR的双特异性抗体,
(b)0.6mg/mL的聚山梨酯80,
(c)75mg/mL的蔗糖,
(d)2000U/mL的PH20,和
(e)20mM的组氨酸-盐酸组氨酸缓冲剂;所述药物组合物的pH为6.0。
在一些实施方案中,如上任一项所述的药物组合物,其包含如下组分:
(a)150mg/mL的特异性结合HGFR和EGFR的双特异性抗体,
(b)0.6mg/mL的聚山梨酯80,
(c)75mg/mL的蔗糖,
(d)10mM的甲硫氨酸,
(e)2000U/mL的PH20,和
(f)20mM的组氨酸-盐酸组氨酸缓冲剂;所述药物组合物的pH为5.0。
在一些实施方案中,如上任一项所述的药物组合物,其包含如下组分:
(a)150mg/mL的特异性结合HGFR和EGFR的双特异性抗体,
(b)0.6mg/mL的聚山梨酯80,
(c)75mg/mL的蔗糖,和
(d)20mM的组氨酸-盐酸组氨酸缓冲剂;所述药物组合物的pH为5.0。
在一些实施方案中,如上任一项所述的药物组合物,其包含如下组分:
(a)120mg/mL的特异性结合HGFR和EGFR的双特异性抗体,
(b)0.6mg/mL的聚山梨酯80,
(c)75mg/mL的蔗糖,和
(d)20mM的组氨酸-盐酸组氨酸缓冲剂;所述药物组合物的pH为6.0。
在一些实施方案中,如上任一项所述的药物组合物,其包含如下组分:
(a)60mg/mL的特异性结合HGFR和EGFR的双特异性抗体,
(b)0.3mg/mL的聚山梨酯80,
(c)37.5mg/mL的蔗糖,
(d)5mM的甲硫氨酸,
(e)1000U/mL的PH20,和
(f)10mM的组氨酸-盐酸组氨酸缓冲剂;所述药物组合物的pH为5.0或6.0。
在一些实施方案中,如上任一项所述的药物组合物,其包含如下组分:
(a)60mg/mL的特异性结合HGFR和EGFR的双特异性抗体,
(b)0.3mg/mL的聚山梨酯80,
(c)37.5mg/mL的蔗糖,
(d)1000U/mL的PH20,和
(e)10mM的组氨酸-盐酸组氨酸缓冲剂;所述药物组合物的pH为6.0。
在一些实施方案中,如上任一项所述的药物组合物,其包含如下组分:
(a)75mg/mL的特异性结合HGFR和EGFR的双特异性抗体,
(b)0.3mg/mL的聚山梨酯80,
(c)37.5mg/mL的蔗糖,
(d)5mM的甲硫氨酸,
(e)1000U/mL的PH20,和
(f)10mM的组氨酸-盐酸组氨酸缓冲剂;所述药物组合物的pH为5.0。
在一些实施方案中,如上任一项所述的药物组合物,其包含如下组分:
(a)75mg/mL的特异性结合HGFR和EGFR的双特异性抗体,
(b)0.3mg/mL的聚山梨酯80,
(c)37.5mg/mL的蔗糖,和
(d)10mM的组氨酸-盐酸组氨酸缓冲剂;所述药物组合物的pH为5.0。
在一些实施方案中,如上任一项所述的药物组合物,其包含如下组分:
(a)60mg/mL的特异性结合HGFR和EGFR的双特异性抗体,
(b)0.3mg/mL的聚山梨酯80,
(c)37.5mg/mL的蔗糖,和
(d)10mM的组氨酸-盐酸组氨酸缓冲剂;所述药物组合物的pH为6.0。
在一些实施方案中,如上所述的药物组合物,所述药物组合物是液体制剂。在一些实施方案中,所述液体制剂的溶剂是水。
本披露还提供一种的冻干制剂,其特征在于所述冻干制剂复溶后可形成如上任一项所述的药物组合物。
本披露还提供一种冻干制剂,其为如上任一项所述的药物组合物的冻干形式制剂。
本披露还提供一种制备冻干制剂的方法,其中包括将如上任一项所述的药物组合物进行冷冻干燥的步骤。在一些实施方案中,如上任一项所述冷冻干燥依次包括预冻、一次干燥和二次干燥的步骤。
本披露还提供一种冻干制剂,所述制剂通过将如上任一项所述的药物组合物经冷冻干燥获得。
本披露还提供一种复溶溶液,其特征在于所述复溶溶液是通过将如上任一项所述的冻干制剂经复溶制备获得。
本披露还提供一种复溶溶液,其为如上任一项所述的冻干制剂的复溶形式制剂。
在一些实施方案中,如上任一项的所述的复溶溶液,其组分和含量与前述的药物组合物的组分和含量相同。
在一些实施方案中,如上任一项所述的复溶溶液,其包含如下组分:
(a)1mg/mL至250mg/mL的所述特异性结合HGFR和EGFR的双特异性抗体,
(b)0.01mg/mL至1.0mg/mL的表面活性剂,
(c)10mg/mL至100mg/mL的糖,和
(d)5mM至100mM的缓冲剂,所述药物组合物的pH为5.0至6.5。
在一些实施方案中,如上任一项所述的复溶溶液,其包含如下组分:
(a)1mg/mL至200mg/mL的所述特异性结合HGFR和EGFR的双特异性抗体,
(b)0.1mg/mL至0.8mg/mL的聚山梨酯80,
(c)30mg/mL至80mg/mL的蔗糖,和
(d)10mM至50mM的组氨酸缓冲剂;所述药物组合物的pH为5.5至6.5。
在一些实施方案中,如上任一项所述的复溶溶液,其包含如下组分:
(a)90mg/mL至110mg/mL的特异性结合HGFR和EGFR的双特异性抗体,
(b)0.4mg/mL至0.8mg/mL的聚山梨酯80,
(c)70mg/mL至80mg/mL mg/mL的蔗糖,和
(d)10mM至20mM的组氨酸-盐酸组氨酸缓冲剂或组氨酸-醋酸组氨酸缓冲剂;所述药物组合物的pH为约6.0。
在一些实施方案中,如上任一项所述的复溶溶液,其包含如下组分:
(a)90mg/mL至110mg/mL的特异性结合HGFR和EGFR的双特异性抗体,
(b)0.6mg/mL的聚山梨酯80,
(c)70mg/mL至80mg/mL的蔗糖,和
(d)20mM的组氨酸-盐酸组氨酸缓冲剂;所述药物组合物的pH为约6.0。
在一些实施方案中,如上任一项所述的复溶溶液,其包含如下组分:
(a)120mg/mL的特异性结合HGFR和EGFR的双特异性抗体,
(b)0.6mg/mL的聚山梨酯80,
(c)70mg/mL至80mg/mL mg/mL的蔗糖,
(d)2000U/mL的PH20,和
(e)20mM的组氨酸-盐酸组氨酸缓冲剂;所述药物组合物的pH为约6.0。
在一些实施方案中,如上任一项所述的复溶溶液,其包含如下组分:
(a)150mg/mL的特异性结合HGFR和EGFR的双特异性抗体,
(b)0.6mg/mL的聚山梨酯80,
(c)70mg/mL至80mg/mL mg/mL的蔗糖,
(d)2000U/mL的PH20,和
(e)20mM的组氨酸-盐酸组氨酸缓冲剂;所述药物组合物的pH为约5.0。
在一些实施方案中,如上任一项所述的复溶溶液,其包含如下组分:
(a)200mg/mL的特异性结合HGFR和EGFR的双特异性抗体,
(b)0.6mg/mL的聚山梨酯80,
(c)70mg/mL至80mg/mL mg/mL的蔗糖,
(d)200mM的盐酸精氨酸,
(e)2000U/mL的PH20,和
(f)20mM的组氨酸-盐酸组氨酸缓冲剂;所述药物组合物的pH为约5.0。
在一些实施方案中,如上任一项所述的复溶溶液,其包含如下组分:
(a)200mg/mL的特异性结合HGFR和EGFR的双特异性抗体,
(b)0.6mg/mL的聚山梨酯80,
(c)70mg/mL至80mg/mL mg/mL的蔗糖,
(d)100mM的盐酸精氨酸,
(e)2000U/mL的PH20,和
(f)20mM的组氨酸-盐酸组氨酸缓冲剂;所述药物组合物的pH为约5.0。
在一些实施方案中,如上任一项所述的复溶溶液,其包含如下组分:
(a)1mg/mL至250mg/mL的所述特异性结合HGFR和EGFR的双特异性抗体,
(b)0.01mg/mL至1.0mg/mL的表面活性剂,
(c)10mg/mL至100mg/mL的糖,和
(d)5mM至100mM的缓冲剂,所述复溶溶液的pH为4.5至6.5。
在一些实施方案中,如上任一项所述的复溶溶液,其包含如下组分:
(a)1mg/mL至200mg/mL的所述特异性结合HGFR和EGFR的双特异性抗体,
(b)0.1mg/mL至0.8mg/mL的聚山梨酯80,
(c)20mg/mL至80mg/mL的蔗糖,和
(d)5mM至50mM的组氨酸缓冲剂;所述药物组合物的pH为4.8至6.2。
在一些实施方案中,如上任一项所述的复溶溶液,其包含如下组分:
(a)80mg/mL至180mg/mL的特异性结合HGFR和EGFR的双特异性抗体,
(b)0.4mg/mL至0.8mg/mL的聚山梨酯80,
(c)60mg/mL至80mg/mL的蔗糖,和
(d)5mM至30mM的组氨酸-盐酸组氨酸缓冲剂或组氨酸-醋酸组氨酸缓冲剂;所述药物组合物的pH为5.0至6.0。
在一些实施方案中,如上任一项所述的复溶溶液,其包含如下组分:
(a)1mg/mL至200mg/mL的所述特异性结合HGFR和EGFR的双特异性抗体,
(b)0.1mg/mL至0.8mg/mL的聚山梨酯80,
(c)20mg/mL至80mg/mL的蔗糖,和
(d)5mM至50mM的组氨酸缓冲剂;所述复溶溶液的pH为5.0至6.5。
在一些实施方案中,如上任一项所述的复溶溶液,其包含如下组分:
(a)50mg/mL至200mg/mL的特异性结合HGFR和EGFR的双特异性抗体,
(b)0.4mg/mL至0.8mg/mL的聚山梨酯80,
(c)60mg/mL至80mg/mL的蔗糖,和
(d)10mM至30mM的组氨酸-盐酸组氨酸缓冲剂或组氨酸-醋酸组氨酸缓冲剂;所述复溶溶液的pH为5.5至6.5。
在一些实施方案中,如上任一项所述的复溶溶液,其包含如下组分:
(a)90mg/mL至110mg/mL的特异性结合HGFR和EGFR的双特异性抗体,
(b)0.4mg/mL至0.8mg/mL的聚山梨酯80,
(c)70mg/mL至80mg/mL的蔗糖,和
(d)15mM至25mM的组氨酸-盐酸组氨酸缓冲剂;所述复溶溶液的pH为5.8至6.2。
在一些实施方案中,如上任一项所述的复溶溶液,其包含如下组分:
(a)约100mg/mL的特异性结合HGFR和EGFR的双特异性抗体,
(b)约0.6mg/mL的聚山梨酯80,
(c)约75mg/mL的蔗糖,和
(d)约20mM的组氨酸-盐酸组氨酸缓冲剂;所述复溶溶液的pH为约6.0。
在一些实施方案中,如上任一项所述的复溶溶液,其包含如下组分:
(a)100mg/mL的特异性结合HGFR和EGFR的双特异性抗体,
(b)0.6mg/mL的聚山梨酯80,
(c)75mg/mL的蔗糖,和
(d)20mM的组氨酸-盐酸组氨酸缓冲剂;所述复溶溶液的pH为6.0。
在一些实施方案中,如上任一项所述的复溶溶液,其包含如下组分:
(a)1mg/mL至250mg/mL的所述特异性结合HGFR和EGFR的双特异性抗体,
(b)0.01mg/mL至1.0mg/mL的表面活性剂,
(c)10mg/mL至100mg/mL的糖,
(d)1mM至300mM的辅料,
(e)10U/mL至3000U/mL的酶,和
(f)5mM至100mM的缓冲剂,所述复溶溶液的pH为4.5至6.5。
在一些实施方案中,如上任一项所述的复溶溶液,其包含如下组分:
(a)1mg/mL至200mg/mL的所述特异性结合HGFR和EGFR的双特异性抗体,
(b)0.1mg/mL至0.8mg/mL的聚山梨酯80,
(c)20mg/mL至80mg/mL的蔗糖,
(d)1mM至200mM的甲硫氨酸,
(e)700U/mL至2400U/mL的透明质酸酶,和
(f)5mM至50mM的组氨酸缓冲剂;所述药物组合物的pH为4.8至6.2。
在一些实施方案中,如上任一项所述的复溶溶液,其包含如下组分:
(a)80mg/mL至180mg/mL的特异性结合HGFR和EGFR的双特异性抗体,
(b)0.4mg/mL至0.8mg/mL的聚山梨酯80,
(c)60mg/mL至80mg/mL的蔗糖,
(d)1mM至20mM的甲硫氨酸,
(e)1600U/mL至2400U/mL的透明质酸酶,和
(f)5mM至30mM的组氨酸-盐酸组氨酸缓冲剂或组氨酸-醋酸组氨酸缓冲剂;所述药物组合物的pH为5.0至6.0。
在一些实施方案中,如上任一项所述的复溶溶液,其包含如下组分:
(a)50mg/mL至200mg/mL的所述特异性结合HGFR和EGFR的双特异性抗体,
(b)0.1mg/mL至0.8mg/mL的聚山梨酯80,
(c)20mg/mL至80mg/mL的蔗糖,
(d)1mM至200mM的甲硫氨酸,
(e)150U/mL至2200U/mL的透明质酸酶,和
(f)5mM至50mM的组氨酸缓冲剂;所述复溶溶液的pH为4.5至6.0。
在一些实施方案中,如上任一项所述的复溶溶液,其包含如下组分:
(a)100mg/mL至200mg/mL的特异性结合HGFR和EGFR的双特异性抗体,
(b)0.4mg/mL至0.8mg/mL的聚山梨酯80,
(c)60mg/mL至80mg/mL的蔗糖,
(d)4mM至20mM的甲硫氨酸,
(e)800U/mL至2200U/mL的透明质酸酶,和
(f)10mM至30mM的组氨酸-盐酸组氨酸缓冲剂或组氨酸-醋酸组氨酸缓冲剂;所述复溶溶液的pH为4.5至5.5。
在一些实施方案中,如上任一项所述的复溶溶液,其包含如下组分:
(a)120mg/mL至180mg/mL的特异性结合HGFR和EGFR的双特异性抗体,
(b)0.4mg/mL至0.8mg/mL的聚山梨酯80,
(c)60mg/mL至80mg/mL的蔗糖,
(d)1mM至20mM的甲硫氨酸,
(e)1600U/mL至2400U/mL的透明质酸酶,和
(f)20mM至30mM的组氨酸-盐酸组氨酸缓冲剂;所述复溶溶液的pH为4.8至5.2。
在一些实施方案中,如上任一项所述的复溶溶液,其包含如下组分:
(a)约150mg/mL的特异性结合HGFR和EGFR的双特异性抗体,
(b)约0.6mg/mL的聚山梨酯80,
(c)约70mg/mL的蔗糖,
(d)约10mM的甲硫氨酸,
(e)约2000U/mL的透明质酸酶,和
(f)约25mM的组氨酸-盐酸组氨酸缓冲剂;所述复溶溶液的pH为约5.0。
在一些实施方案中,如上任一项所述的复溶溶液,其包含如下组分:
(a)150mg/mL的特异性结合HGFR和EGFR的双特异性抗体,
(b)0.6mg/mL的聚山梨酯80,
(c)70mg/mL的蔗糖,
(d)10mM的甲硫氨酸,
(e)2000U/mL的透明质酸酶,和
(f)25mM的组氨酸-盐酸组氨酸缓冲剂;所述复溶溶液的pH为5.0。
在一些实施方案中,如上任一项所述的复溶溶液,其包含如下组分:
(a)50mg/mL至100mg/mL的特异性结合HGFR和EGFR的双特异性抗体,
(b)0.2mg/mL至0.4mg/mL的聚山梨酯80,
(c)20mg/mL至50mg/mL的蔗糖,
(d)1mM至10mM的甲硫氨酸,
(e)600U/mL至1000U/mL的透明质酸酶,和
(f)5mM至15mM的组氨酸-盐酸组氨酸缓冲剂或组氨酸-醋酸组氨酸缓冲剂;所述复溶溶液的pH为4.5至5.5。
在一些实施方案中,如上任一项所述的复溶溶液,其包含如下组分:
(a)50mg/mL至75mg/mL的特异性结合HGFR和EGFR的双特异性抗体,
(b)0.2mg/mL至0.3mg/mL的聚山梨酯80,
(c)20mg/mL至35mg/mL的蔗糖,
(d)1mM至10mM的甲硫氨酸,
(e)720U/mL至880U/mL的透明质酸酶,和
(f)8mM至12mM的组氨酸-盐酸组氨酸缓冲剂;所述复溶溶液的pH为4.8至5.2。
在一些实施方案中,如上任一项所述的复溶溶液,其包含如下组分:
(a)约60mg/mL的特异性结合HGFR和EGFR的双特异性抗体,
(b)约0.24mg/mL的聚山梨酯80,
(c)约28mg/mL的蔗糖,
(d)约4mM的甲硫氨酸,
(e)约800U/mL的透明质酸酶,和
(f)约10mM的组氨酸-盐酸组氨酸缓冲剂;所述复溶溶液的pH为约5.0。
在一些实施方案中,如上任一项所述的复溶溶液,其包含如下组分:
(a)60mg/mL的特异性结合HGFR和EGFR的双特异性抗体,
(b)0.24mg/mL的聚山梨酯80,
(c)28mg/mL的蔗糖,
(d)4mM的甲硫氨酸,
(e)800U/mL的透明质酸酶,和
(f)10mM的组氨酸-盐酸组氨酸缓冲剂;所述复溶溶液的pH为5.0。
在一些实施方案中,如上任一项所述的复溶溶液,其包含如下组分:
(a)50mg/mL至250mg/mL的所述特异性结合HGFR和EGFR的双特异性抗体,
(b)0.1mg/mL至0.8mg/mL的聚山梨酯80,
(c)20mg/mL至80mg/mL的蔗糖,
(d)80mM至240mM的盐酸精氨酸,和
(e)5mM至50mM的组氨酸缓冲剂;所述复溶溶液的pH为4.5至6.0。
在一些实施方案中,如上任一项所述的复溶溶液,其包含如下组分:
(a)160mg/mL至240mg/mL的特异性结合HGFR和EGFR的双特异性抗体,
(b)0.4mg/mL至0.8mg/mL的聚山梨酯80,
(c)60mg/mL至80mg/mL的蔗糖,
(d)160mM至240mM的盐酸精氨酸,和
(e)10mM至30mM的组氨酸-盐酸组氨酸缓冲剂或组氨酸-醋酸组氨酸缓冲剂;所述复溶溶液的pH为4.5至5.5。
在一些实施方案中,如上任一项所述的复溶溶液,其包含如下组分:
(a)180mg/mL至220mg/mL的特异性结合HGFR和EGFR的双特异性抗体,
(b)0.4mg/mL至0.8mg/mL的聚山梨酯80,
(c)70mg/mL至80mg/mL的蔗糖,
(d)180mM至220mM的盐酸精氨酸,和
(e)15mM至25mM的组氨酸-盐酸组氨酸缓冲剂;所述复溶溶液的pH为4.8至5.2。
在一些实施方案中,如上任一项所述的复溶溶液,其包含如下组分:
(a)约200mg/mL的特异性结合HGFR和EGFR的双特异性抗体,
(b)约0.6mg/mL的聚山梨酯80,
(c)约75mg/mL的蔗糖,
(d)约200mM的盐酸精氨酸,和
(e)约20mM的组氨酸-盐酸组氨酸缓冲剂;所述复溶溶液的pH为约5.0。
在一些实施方案中,如上任一项所述的复溶溶液,其包含如下组分:
(a)200mg/mL的特异性结合HGFR和EGFR的双特异性抗体,
(b)0.6mg/mL的聚山梨酯80,
(c)75mg/mL的蔗糖,
(d)200mM的盐酸精氨酸,和
(e)20mM的组氨酸-盐酸组氨酸缓冲剂;所述复溶溶液的pH为5.0。
在一些实施方案中,如上任一项所述的复溶溶液,其包含如下组分:
(a)50mg/mL至150mg/mL的特异性结合HGFR和EGFR的双特异性抗体,
(b)0.2mg/mL至0.4mg/mL的聚山梨酯80,
(c)20mg/mL至50mg/mL的蔗糖,
(d)80mM至120mM的盐酸精氨酸,和
(e)5mM至15mM的组氨酸-盐酸组氨酸缓冲剂或组氨酸-醋酸组氨酸缓冲剂;所述复溶溶液的pH为4.5至5.5。
在一些实施方案中,如上任一项所述的复溶溶液,其包含如下组分:
(a)80mg/mL至120mg/mL的特异性结合HGFR和EGFR的双特异性抗体,
(b)0.2mg/mL至0.4mg/mL的聚山梨酯80,
(c)30mg/mL至50mg/mL的蔗糖,
(d)90mM至110mM的盐酸精氨酸,和
(e)15mM至25mM的组氨酸-盐酸组氨酸缓冲剂;所述复溶溶液的pH为4.8至5.2。
在一些实施方案中,如上任一项所述的复溶溶液,其包含如下组分:
(a)约100mg/mL的特异性结合HGFR和EGFR的双特异性抗体,
(b)约0.3mg/mL的聚山梨酯80,
(c)约37.5mg/mL的蔗糖,
(d)约100mM的盐酸精氨酸,和
(e)约10mM的组氨酸-盐酸组氨酸缓冲剂;所述复溶溶液的pH为约5.0。
在一些实施方案中,如上任一项所述的复溶溶液,其包含如下组分:
(a)100mg/mL的特异性结合HGFR和EGFR的双特异性抗体,
(b)0.3mg/mL的聚山梨酯80,
(c)37.5mg/mL的蔗糖,
(d)100mM的盐酸精氨酸,和
(e)10mM的组氨酸-盐酸组氨酸缓冲剂;所述复溶溶液的pH为5.0。
在一些实施方案中,如上任一项所述的复溶溶液,其包含如下组分:
(a)120mg/mL的特异性结合HGFR和EGFR的双特异性抗体,
(b)0.6mg/mL的聚山梨酯80,
(c)75mg/mL的蔗糖,
(d)10mM的甲硫氨酸,
(e)2000U/mL的PH20,和
(f)20mM的组氨酸-盐酸组氨酸缓冲剂;所述复溶溶液的pH为5.0或6.0。
在一些实施方案中,如上任一项所述的复溶溶液,其包含如下组分:
(a)120mg/mL的特异性结合HGFR和EGFR的双特异性抗体,
(b)0.6mg/mL的聚山梨酯80,
(c)75mg/mL的蔗糖,
(d)2000U/mL的PH20,和
(e)20mM的组氨酸-盐酸组氨酸缓冲剂;所述复溶溶液的pH为6.0。
在一些实施方案中,如上任一项所述的复溶溶液,其包含如下组分:
(a)150mg/mL的特异性结合HGFR和EGFR的双特异性抗体,
(b)0.6mg/mL的聚山梨酯80,
(c)75mg/mL的蔗糖,
(d)10mM的甲硫氨酸,
(e)2000U/mL的PH20,和
(f)20mM的组氨酸-盐酸组氨酸缓冲剂;所述复溶溶液的pH为5.0。
在一些实施方案中,如上任一项所述的复溶溶液,其包含如下组分:
(a)150mg/mL的特异性结合HGFR和EGFR的双特异性抗体,
(b)0.6mg/mL的聚山梨酯80,
(c)75mg/mL的蔗糖,和
(d)20mM的组氨酸-盐酸组氨酸缓冲剂;所述复溶溶液的pH为5.0。
在一些实施方案中,如上任一项所述的复溶溶液,其包含如下组分:
(a)120mg/mL的特异性结合HGFR和EGFR的双特异性抗体,
(b)0.6mg/mL的聚山梨酯80,
(c)75mg/mL的蔗糖,和
(d)20mM的组氨酸-盐酸组氨酸缓冲剂;所述复溶溶液的pH为6.0。
在一些实施方案中,如上任一项所述的复溶溶液,其包含如下组分:
(a)60mg/mL的特异性结合HGFR和EGFR的双特异性抗体,
(b)0.3mg/mL的聚山梨酯80,
(c)37.5mg/mL的蔗糖,
(d)5mM的甲硫氨酸,
(e)1000U/mL的PH20,和
(f)10mM的组氨酸-盐酸组氨酸缓冲剂;所述复溶溶液的pH为5.0或6.0。
在一些实施方案中,如上任一项所述的复溶溶液,其包含如下组分:
(a)60mg/mL的特异性结合HGFR和EGFR的双特异性抗体,
(b)0.3mg/mL的聚山梨酯80,
(c)37.5mg/mL的蔗糖,
(d)1000U/mL的PH20,和
(e)10mM的组氨酸-盐酸组氨酸缓冲剂;所述复溶溶液的pH为6.0。
在一些实施方案中,如上任一项所述的复溶溶液,其包含如下组分:
(a)75mg/mL的特异性结合HGFR和EGFR的双特异性抗体,
(b)0.3mg/mL的聚山梨酯80,
(c)37.5mg/mL的蔗糖,
(d)5mM的甲硫氨酸,
(e)1000U/mL的PH20,和
(f)10mM的组氨酸-盐酸组氨酸缓冲剂;所述复溶溶液的pH为5.0。
在一些实施方案中,如上任一项所述的复溶溶液,其包含如下组分:
(a)75mg/mL的特异性结合HGFR和EGFR的双特异性抗体,
(b)0.3mg/mL的聚山梨酯80,
(c)37.5mg/mL的蔗糖,和
(d)10mM的组氨酸-盐酸组氨酸缓冲剂;所述复溶溶液的pH为5.0。
在一些实施方案中,如上任一项所述的复溶溶液,其包含如下组分:
(a)60mg/mL的特异性结合HGFR和EGFR的双特异性抗体,
(b)0.3mg/mL的聚山梨酯80,
(c)37.5mg/mL的蔗糖,和
(d)10mM的组氨酸-盐酸组氨酸缓冲剂;所述复溶溶液的pH为6.0。
在一些实施方案中,如上任一项所述的药物组合物或复溶溶液,其为静脉注射制剂、皮下注射制剂、腹腔注射制剂或肌肉注射制剂。在一些实施方案中,如上任一项所述的药物组合物或复溶溶液,其为静脉注射制剂。在一些实施方案中,如上任一项所述的药物组合物或复溶溶液,其为皮下注射制剂。
在一些实施方案中,如上任一项所述的药物组合物或复溶溶液,其适用于静脉注射、皮下注射、腹腔注射或肌肉注射。在一些实施方案中,如上任一项所述的药物组合物或复溶溶液,其适用于静脉注射。在一些实施方案中,如上任一项所述的药物组合物或复溶溶液,其适用于皮下注射。
在一些实施方案中,如上任一项所述的药物组合物或复溶溶液或冻干制剂,其用于制备静脉注射、皮下注射、腹腔注射或肌肉注射的药物。在一些实施方案中,如上任一项所述的药物组合物或复溶溶液或冻干制剂,其用于制备静脉注射的药物。在一些实施方案中,如上任一项所述的药物组合物或复溶溶液或冻干制剂,其用于制备皮下注射的药物。
本披露还提供一种药盒,其包括至少一个容器,各容器独立地包含如上任一项所述的药物组合物、如上任一项所述的冻干制剂或如上任一项所述的复溶溶液。
在一些实施方案中,本披露还提供诊断、治疗、缓解受试者病症的方法,包含向所述受试者施用有效量的如上任一项所述的药物组合物、如上任一项所述的冻干制剂、如上任一项所述的复溶溶液或如上任一项所述的药盒。
在一些实施方案中,本披露还提供一种治疗或预防疾病的方法,所述方法包括向受试者施用治疗有效量的如上任一项所述的药物组合物、如上任一项所述的冻干制剂、如上任一项所述的复溶溶液或如上任一项所述的药盒。
在一些实施方案中,本披露还提供如上任一项所述的药物组合物、如上任一项所述的冻干制剂、如上任一项所述的复溶溶液或如上任一项所述的药盒,其用于治疗或预防疾病。
在一个方面,本披露还提供如前任一项所述的药物组合物、如上任一项所述的冻干制剂、如上任一项所述的复溶溶液或如上任一项所述的药盒在制备用于预防或治疗疾病或病症的药物中的用途。
在一些具体实施方案中,前面任一项所述的疾病是肿瘤。在一些实施方式中,所述肿瘤选自肺癌(包括非小细胞肺癌和小细胞肺癌)、乳腺癌、胰腺癌、结直肠癌(包括结肠癌和直肠癌)、肉瘤、肾细胞癌、肝细胞癌、胃癌、卵巢癌、膀胱癌、头颈癌和胶质母细胞瘤。在一些实施方式中,所述肿瘤选自肺癌、乳腺癌、胰腺癌、结肠癌、头颈癌、胃癌和胶质母细胞瘤。
在一些具体的实施方式中,所述肺癌为非小细胞肺癌。
在一些具体的实施方式中,所述肺癌为转移性非小细胞肺癌。
在一些具体的实施方式中,所述肺癌为小细胞肺癌。
在一些具体的实施方式中,所述肺癌为人肺腺癌。
在一些具体的实施方式中,所述肺癌为胃癌。
在一些具体的实施方式中,所述肿瘤为EGFR和/或HGFR相关的肿瘤。
图1:Format1的结构示意图。
图2:Format2的结构示意图。
图3:抗体与EGFR CHO-S细胞结合活性实验结果图,其中,附图纵坐标为平均荧光强度(简称MFI,下同)。
图4:抗体与HGFR CHO-S细胞结合活性实验结果图。
图5:抗体与H1975-HGF细胞结合活性实验结果图。
图6:抗体与MKN-45细胞结合活性实验结果图。
图7:抗体对细胞EGFR磷酸化的抑制实验结果。
图8:抗体对细胞HGFR磷酸化的抑制实验结果。
图9:抗体对细胞AKT磷酸化的抑制实验结果。
图10:抗体对细胞表面HGFR的减少实验结果。
图11:抗体对SNU-5细胞的增殖抑制。
图12:抗体对Hs746T细胞的ADCC杀伤实验结果。
图13:抗体对H292细胞的ADCC杀伤实验结果。
图14:抗体抑制HCC827小鼠肿瘤细胞的生长。
术语
为了更容易理解本披露,以下具体定义了某些技术和科学术语。除非在本文中另有明确定义,否则本文使用的所有其它技术和科学术语都具有本披露所属领域的一般技术人员通常理解的含义。
本披露所用的单数形式“一个”、“一种”和“所述”包括复数指代,除非上下文清楚表明并非如此。
除非上下文另外清楚要求,否则在专利说明书和权利要求书中,应将词语“包含”、“具有”、“包括”等理解为“包括但不仅限于”的意义,而不是排他性或穷举性意义。
“任选”或“任选地”意味着随后所描述地事件或环境可以但不必发生,该说明包括该事件或环境发生或不发生地场合。
本领域技术人员应当理解,当参考数值范围、截止值或特定值使用时,“约”可以表示在1个或多于1个标准偏差之内。或者,“约”可以表示相差最高达20%的范围(即±20%)。由于本文所用的许多数值是通过实验确定的,因此本领域的技术人员应当理解,此类确定可以在不同实验之间有差异并且通常在不同实验之间有差异。由于这种内在差异,认为本文所用的值不应受到过度限制。因此,术语“约”用于涵盖与指定值相差±20%或更小的变化、±10%或更小的变化、±5%或更小的变化、±1%或更小的变化、±0.5%或更小的变化、或者±0.1%或更小的变化。
尽管本披露提供了含量范围或含量值,但本领域一般技术人员理解,所述含量范围或含量值涵盖了所测定具体值的可接受误差范围。
本披露所用氨基酸三字母代码和单字母代码如J.biol.chem,243,p3558(1968)中所述。
“HGFR”应作广泛的理解,旨在涵盖HGFR在哺乳动物体内各阶段中的各种形式的分子,例如但不限于HGFR基因在扩增、复制、转录、剪接、加工、翻译、修饰过程中所产生的分子(例如前体HGFR、成熟HGFR、膜表达的HGFR、HGFR剪接变体、修饰的HGFR、或其片段);该术语也涵盖人工制备的或体外表达的HGFR。
“EGFR”应作广泛的理解,旨在涵盖EGFR在哺乳动物体内各阶段中的各种形式的分子,例如但不限于EGFR基因在扩增、复制、转录、剪接、加工、翻译、修饰过程中所产生的分子(例如前体EGFR、成熟EGFR、膜表达的EGFR、EGFR剪接变体、修饰的EGFR、或其片段);该术语也涵盖人工制备的或体外表达的EGFR。
术语“氨基酸”是指天然存在的和合成的氨基酸,以及以与天然存在的氨基酸类似的方式起作用的氨基酸类似物和氨基酸模拟物。天然存在的氨基酸是由遗传密码编码的那些氨基酸,以及后来修饰的那些氨基酸,例如羟脯氨酸、γ-羧基谷氨酸和O-磷酸丝氨酸。氨基酸类似物是指与天然存在的氨基酸具有相同基本化学结构(即与氢、羧基、氨基和R基团结合的α碳)的化合物,例如高丝氨酸、正亮氨酸、甲硫氨酸亚砜、甲硫氨酸甲基锍。此类类似物具有修饰的R基团(例如,正亮氨酸)或修饰的肽骨架,但保留与天然存在的氨基酸相同的基本化学结构。氨基酸模拟物是指具有与氨基酸的一般化学结构不同的结构,但是以与天然存在的氨基酸类似的方式起作用的化学化合物。
术语“抗体”以最广义使用,并且涵盖各种抗体结构,包括但不限于单克隆抗体,多克隆抗体;单特异性抗体,多特异性抗体(例如双特异性抗体),全长抗体和抗体片段(或抗原结合片段,或抗原结合部分),只要它们展现出期望的抗原结合活性。“天然抗体”指天然存在的免疫球蛋白分子。例如,天然IgG抗体是约150,000道尔顿的异四聚体蛋白,由二硫键结合的两条轻链和两条重链构成。从N至C端,每条重链具有一个可变区(VH),又称作可变重域、重链可变区,接着是三个恒定域(CH1、CH2和CH3)。类似地,从N至C端,每条轻链具有一个可变区(VL),又称作可变轻域,或轻链可变域,接着是一个恒定轻域(轻链恒定区、CL)。
术语“双特异性抗体”指能够对两个不同抗原或同一抗原的两个不同抗原表位特异性结合的抗体(包括抗体或其抗原结合片段,如单链抗体)。现有技术已公开了各种结构的双特异性抗体。根据IgG分子的完整性可分为IgG样双特异性抗体和抗体片段型双特异性抗体。根据抗原结合区域的数量可分为二价、三价、四价或更多价的双特异性抗体。根据结构是否对称可分为对称结构双特异性抗体
和不对称结构双特异性抗体。其中,片段型双特异性抗体,例如缺乏Fc片段的Fab片段,其通过将2个或多个Fab片段结合在一个分子中形成双特异性抗体,其具有较低的免疫原性,且分子量小,具有较高的肿瘤组织渗透性,该类型的典型的抗体结构如F(ab′)2、scFv-Fab、(scFv)2-Fab等。IgG样双特异性抗体(例如具有Fc片段),这类抗体相对分子量较大,Fc片段有助于抗体的纯化,并提高其溶解性、稳定性,Fc部分还可能会与受体FcRn结合,增加抗体血清半衰期。典型的双特异性抗体结构模型如KiH、CrossMAb、Triomab quadroma、FcΔAdp、ART-Ig、BiMAb、Biclonics、BEAT、DuoBody、Azymetric、XmAb、2:1TCBs、1Fab-IgG TDB、FynomAb、two-in-one/DAF、scFv-Fab-IgG、DART-Fc、LP-DART、CODV-Fab-TL、HLE-BiTE、F(ab)2-CrossMAb、IgG-(scFv)2、Bs4Ab、DVD-Ig、Tetravalent-DART-Fc、(scFv)4-Fc、CODV-Ig、mAb2、F(ab)4-CrossMAb等双特异性抗体(参见Aran F.Labrijn等,Nature Reviews Drug Discovery volume 18,pages585–608(2019);Chen S1等,J Immunol Res.2019 Feb 11;2019:4516041)。
术语“可变区”或“可变域”指抗原结合分子中结合抗原的域。本文中,特异性结合HGFR的抗原结合模块1中的重链可变区标示为M-VH,轻链可变区标示为M-VL;特异性结合EGFR的抗原结合模块2中的重链可变区标示为E-VH,轻链可变区标示为E-VL。VH和VL各包含四个保守的框架区(FR)和三个互补决定区(CDR)。其中,术语“互补决定区”或“CDR”指可变结构域内主要促成与抗原结合的区域;“框架”或“FR”是指除CDR残基之外的可变结构域残基。VH包含3个CDR区:HCDR1、HCDR2和HCDR3;VL包含3个CDR区:LCDR1、LCDR2和LCDR3。本文中,M-VH中的3个CDR区分别标示为M-HCDR1、M-HCDR2和M-HCDR3;M-VL中的3个CDR区分别标示为M-LCDR1、M-LCDR2和M-LCDR3;E-VH中的3个CDR区分别标示为E-HCDR1、E-HCDR2和E-HCDR3;E-VL中的3个CDR区分别标示为E-LCDR1、E-LCDR2和E-LCDR3。每个VH和VL由从氨基末端排到羧基末端按以下顺序排列的三个CDR和四个FR构成:FR1、CDR1、FR2、CDR2、FR3、CDR3、FR4。单个VH或VL可能足以赋予抗原结合特异性。
可以通过各种公知方案来确定CDR的氨基酸序列边界,例如:“Kabat”编号规则(参见Kabat等(1991),“Sequences of Proteins of Immunological Interest”,第5版,Public Health Service,National Institutes of Health,Bethesda,MD)、“Chothia”编号规则、“ABM”编号规则、“contact”编号规则(参见Martin,ACR.Protein Sequence and Structure Analysis of Antibody Variable Domains[J].2001)和ImMunoGenTics(IMGT)编号规则(Lefranc,M.P.等,Dev.Comp.Immunol.,27,55-77(2003);Front Immunol.2018 Oct 16;9:2278)等;各种编号***之间的对应关系是本领域技术人员熟知的。本披露的编号规则如下表1中所示。
表1.CDR编号***之间的关系
除非另有说明,本披露实施例中的可变区和CDR序列均适用“Kabat”编号规则。尽管在具体的实施方案中,采用了Kabat编号规则来限定氨基酸残基,但是其他编号***所的对应技术方案将视为等同技术方案。
术语“抗体片段”指不同于完整抗体的分子,其包含完整抗体的部分,所述部分保留了完整抗体的抗原结合能力。抗体片段的实例包括但不限于Fv、Fab、Fab′、Fab′-SH、F(ab′)2、单域抗体、单链Fab(scFab)、双抗体、线性抗体、单链抗体分子(例如scFv);以及由抗体片段形成的多特异性抗体。
Fab指由免疫球蛋白的VH和CH1(Fab重链)与VL和CL(Fab轻链)组成的蛋白质。
Fv指由免疫球蛋白的VH与VL组成的抗原结合域。
本披露中,在一些实施方案中,所述第一Fab具有Fab的结构。而在经替换的Fab中,CH1与CL分别被Titin链或Obscurin链替代。
术语“Fc区”或“片段可结晶区”用于定义抗体重链的C末端区域,包括天然Fc区和改造的Fc区。在一些实施方式中,Fc区包含了相同或不同的两个亚基。在一些实施方式中,人IgG重链的Fc区定义为从Cys226位置处的氨基酸残基或从Pro230延伸至其羧基末端。用于本文所述抗体的合适天然序列Fc区包括人IgG1、IgG2(IgG2A、IgG2B)、IgG3和IgG4。除非另有说明,Fc区的编号规则为EU索引。
术语“Titin链”是指Titin蛋白中一段长度为78-118个氨基酸的片段,其包含Titin Ig-样152结构域的肽段或其功能变体。所述Titin链能够与Obscurin链形成二聚化复合物。
术语“Obscurin链”是指Obscurin蛋白上一段长度为87-117个氨基酸的片段,其包含Obscurin Ig-样1结构域的肽段或其功能变体;或是指Obscurin-样1蛋白上一段长度为78-118个氨基酸的片段,其包含Obscurin-样Ig-样1结构域的肽段或其功能变体。所述Obscurin链能够与Titin链结合形成二聚化复合物。本披露的Titin链与Obscurin链可用于替换Fab中的CH1和CL,形成经替换的Fab(Fab-S)。该替换不影响抗原结合分子与抗原的结合。
术语“抗原结合模块”指特异性结合目标抗原的多肽分子。抗原结合模块包括如本文所述的抗体及其片段。具体的抗原结合模块包括抗体的抗原结合域,其包含抗体重链可变区和抗体轻链可变区。术语“特异性结合HGFR的抗原结合模块”是指能够以足够的亲和力结合HGFR或其表位的模块。在某些实施例中,特异性结合HGFR的抗原结合模块具有以下的平衡解离常数(KD):<约1μM、<约100nM或<约10nM或更小,其是通过Biacore方法测量的。在某些实施例中,特异性结合HGFR的抗原结合模块结合来自不同物种的HGFR中的保守表位。术语“特异性结合EGFR的抗原结合模块”是指能够以足够的亲和力结合EGFR或其表位的模块,使得含有该模块的分子可用作靶向EGFR的诊断剂和/或治疗剂。在某些实施例中,特异性结合EGFR的抗原结合模块具有以下的平衡解离常数(KD):<约1μM、<约100nM、<约10nM或更小,其是通过Biacore方法测量的。在某些实施例中,特异性结合EGFR的抗原结合模块结合来自不同物种的EGFR中的保守表位。抗原结合模块包括如本文定义的抗体片段,例如Fab,经替换的Fab或Fv。
在上下文中,“抗原结合模块1”、“抗原结合模块2”、“连接子1”、“连接子2”中的序数词,其目的仅在于区分不同的技术特征、化学实体;而不限定任何顺序、水平、数量。
术语“连接子”指连接两个多肽片段的连接单元。在本文中,同一或不同结构式中出现的连接子可以是相同或不同的。连接子可以是肽连接子,其包含一个或更多个氨基酸,典型的约1-30个、2-24个或3-15个氨基酸。应用于本文的连接子可以是相同或不同的。当“-”出现在结构式中,其表示两侧的单元直接通过共价键连接。当术语“键”出现在结构单元,其表示该单元两侧的单元直接连接。
除非另有说明,本披露实施例中的可变区和CDR序列均适用“Kabat”编号规则。
“药物组合物”表示含有一种或多种本文所述双特异性抗体,以及其他组分例如生理学/可药用的载体和赋形剂。药物组合物的目的是促进对生物体的给药,利于活性成分的吸收进而发挥生物活性。本披露中,“药物组合物”和“制剂”并不互相排斥。
“有效量”包含足以改善或预防医学病症的症状或病症的量。有效量还意指足以允许或促进诊断的量。用于特定受试者或兽医学受试者的有效量可依据以下因素而变化:如待治疗的病症、受试者的总体健康情况、给药的方法途径和剂量以及副作用严重性。有效量可以是避免显著副作用或毒性作用的最大剂量或给药方案。
“药学可接受的载体”或“药学可接受的赋形剂”包括当与活性成分组合时,允许该成分保留生物学活性并且不与受试者的免疫***反应的任何材料。例子包括但不限于任何标准药物载体,例如磷酸盐缓冲盐水溶液、水、乳剂如油/水乳剂、
和各种类型的润湿剂。在一些实施例中,用于气雾剂或肠胃外施用的稀释剂是磷酸盐缓冲盐水(PBS)或生理(0.9%)盐水。包含此类载体的组合物通过众所周知的常规方法配制。
“缓冲剂”指通过其酸-碱共轭组分的作用而耐受pH变化的缓冲剂。将pH控制在适当范围中的缓冲剂的例子包括醋酸盐、琥珀酸盐、葡萄糖酸盐、组氨酸、草酸盐、乳酸盐、磷酸盐、枸橼酸盐、酒石酸盐、延胡索酸盐、甘氨酰甘氨酸和其它有机酸缓冲剂。
“组氨酸缓冲剂”是包含组氨酸的缓冲剂。组氨酸缓冲剂的实例包括组氨酸-盐酸组氨酸,组氨酸-醋酸组氨酸,组氨酸-磷酸组氨酸,组氨酸-硫酸组氨酸等缓冲剂,优选组氨酸-盐酸组氨酸缓冲剂。组氨酸-盐酸组氨酸缓冲剂可由组氨酸与盐酸配制而成,或者由组氨酸与盐酸组氨酸配制而成。
“枸橼酸盐缓冲剂”是包括枸橼酸根离子的缓冲剂。枸橼酸盐缓冲剂的实例包括枸橼酸-枸橼酸钠、枸橼酸-枸橼酸钾、枸橼酸-枸橼酸钙、枸橼酸-枸橼酸镁等。优选的枸橼酸盐缓冲剂是枸橼酸-枸橼酸钠。
“琥珀酸盐缓冲剂”是包括琥珀酸根离子的缓冲剂。琥珀酸盐缓冲剂的实例包括琥珀酸-琥珀酸钠盐、琥珀酸-琥珀酸钾、琥珀酸-琥珀酸钙盐等。优选的琥珀酸盐缓冲剂是琥珀酸-琥珀酸钠盐。示例性的,所述的琥珀酸-琥珀酸钠可由琥珀酸与氢氧化钠配制而成,或由琥珀酸与琥珀酸钠盐配制而成。
“磷酸盐缓冲剂”是包括磷酸根离子的缓冲剂。磷酸盐缓冲剂的实例包括柠檬酸-磷酸氢二钠、磷酸氢二钠-磷酸二氢钠、磷酸氢二钠-磷酸二氢钾、磷酸氢二钠-枸橼酸等。优选的磷酸盐缓冲剂是柠檬酸-磷酸氢二钠。
“醋酸盐缓冲剂”是包括醋酸根离子的缓冲剂。醋酸盐缓冲剂的实例包括醋酸-醋酸钠、组氨酸-醋酸组氨酸、醋酸-醋酸钾、醋酸-醋酸钙、醋酸-醋酸镁等。优选的醋酸盐缓冲剂是醋酸-醋酸钠。
“泊洛沙姆(poloxamer)”是环氧乙烷和环氧丙烷的嵌段共聚物,其是水溶性的并在药物制剂中用作表面活性剂。泊洛沙姆的实例包括泊洛沙姆188(P188)。
“冻干制剂”表示液体或溶液形式的药物组合物或液体或溶液制剂经真空冷冻干燥步骤之后获得的制剂或药物组合物。通常,冷冻干燥包括预冻,一次干燥,二次干燥。预冻目的是冷冻产品,获得结晶固体;在一些实施方案中,预冻温度设定为-45℃,预冻速度设定为1℃/min。一次干燥也称为主干燥,是样品冷冻干燥的主要阶段,目的是移除产品里冰的同时,保持产品形状,最小程度的减少对产品的破坏;若一次干燥的温度和真空度选择不当,会导致制品的塌陷;较高的温度和真空度均会加快冻干效率,但同时也会增加制品塌陷的风险。在一些实施方案中,一次干燥的温度可为本领域常规的温度,例如-30℃-0℃。二次干燥也称为解析干燥,是通过抽极限真空(0.01mbar)和升高温度(20℃-40℃)除去制品中结合水的主要步骤。由于大多数生物制品对温度比较敏感,所以二次干燥
温度常选择在温度范围的低点,例如25℃。冷冻干燥的时间与冷冻机、冻干制剂剂量、冻干药剂的容器有关。这种时间的调整是为本领域技术人员所熟知的。
本披露中所述药物组合物的溶液形式,若无特殊说明,其中的溶剂均为水。本披露所述的药物组合物能够达到一种稳定的效果:其中的抗体在贮藏后基本上保留其物理稳定性和/或化学稳定性和/或生物学活性的药物组合物,优选地,药物组合物在贮藏后基本上保留其物理和化学稳定性以及其生物学活性。贮藏期一般基于药物组合物的预定保存期来选择。目前有多种测量蛋白质稳定性的分析技术,可测量在选定温度贮藏选定时间段后的稳定性。
“置换”是指溶解抗体蛋白的溶剂体系的置换,例如,使用稳定制剂的缓冲体系经物理操作方式将含抗体蛋白的高盐或高渗溶剂体系置换,从而使抗体蛋白存在于稳定制剂中。所称物理操作方式包括但不限于超滤、透析或离心。
稳定的制剂是在下述情况下没有观察到显著变化的制剂:在冷藏温度(2-8℃)保存至少3个月、优选6个月、更优选1年,且甚至更优选地多达2年。另外,稳定的液体制剂包括这样的液体制剂:其在包括25℃的温度保存包括24小时、7天、1个月、3个月或6个月在内的时段后表现出期望的特征。还包括40℃的温度保存包括4周、1个月、3个月或6个月在内的时段后表现出期望的特征。稳定性的典型的例子:通过SEC-HPLC测得,通常不超过约20%或10%、优选不超过约5%的抗体发生聚集或降解。通过视觉分析,制剂是淡黄色近无色澄明液体或者无色澄明液体,或澄清至稍微乳白色。所述制剂的浓度、pH和重量克分子渗透压浓度具有不超过±10%变化,优选不超过±5%的变化。所述制剂通常形成不超过约10%、优选不超过约5%的聚集。
如果在目检颜色和/或澄清度后,或者通过UV光散射、尺寸排阻色谱法(SEC)和动态光散射(DLS)测得,抗体没有显示出显著的聚集增加、沉淀和/或变性,那么所述抗体在药物制剂中“保留它的物理稳定性”。蛋白构象的变化可以通过荧光光谱法(其确定蛋白三级结构)和通过FTIR光谱法(其确定蛋白二级结构)来评价。
如果抗体没有显示出显著的化学改变,那么所述抗体在药物制剂中“保留它的化学稳定性”。通过检测和定量化学上改变的形式的蛋白,可以评估化学稳定性。经常改变蛋白化学结构的降解过程包括水解或截短(通过诸如尺寸排阻色谱法和CE-SDS等方法来评价)、氧化(通过诸如与质谱法或MALDI/TOF/MS结合的肽谱法等方法来评价)、脱酰胺作用(通过诸如离子交换色谱法、毛细管等电聚焦、肽谱法、异天冬氨酸测量等方法来评价)和异构化(通过测量异天冬氨酸含量、肽谱法等来评价)。
如果抗体在给定时间的生物活性是在制备药物制剂时表现出的生物活性的预定范围内,那么所述抗体在药物制剂中“保留它的生物活性”。
“施用”、“给予”和“处理”,当其应用于动物、人、实验受试者、细胞、
组织、器官或生物流体时,是指外源性药物、治疗剂、诊断剂或组合物与动物、人、受试者、细胞、组织、器官或生物流体的接触。“施用”、“给予”和“处理”可以指例如治疗、药物代谢动力学、诊断、研究和实验方法。细胞的处理包括试剂与细胞的接触,以及试剂与流体的接触,其中所述流体与细胞接触。“施用”、“给予”和“处理”还意指通过试剂、诊断、结合组合物或通过另一种细胞体外和离体处理例如细胞。“处理”当其应用于人、兽医学或研究受试者时,是指治疗处理、预防或预防性措施,研究和诊断应用。
“治疗”意指给予患者内用或外用治疗剂,例如包含本披露的任一种的复溶溶液,所述患者具有一种或多种疾病症状,而已知所述治疗剂对这些症状具有治疗作用。通常,在受治疗患者或群体中以有效缓解一种或多种疾病症状的量给予治疗剂,以诱导这类症状退化或抑制这类症状发展到任何临床有测量的程度。有效缓解任何具体疾病症状的治疗剂的量(也称作“治疗有效量”)可根据多种因素变化,例如患者的疾病状态、年龄和体重,以及药物在患者产生需要疗效的能力。通过医生或其它专业卫生保健人士通常用于评价该症状的严重性或进展状况的任何临床检测方法,可评价疾病症状是否已被减轻。尽管本披露的实施方案(例如治疗方法或制品)在缓解每个目标疾病症状方面可能无效,但是根据本领域已知的任何统计学检验方法如Student t检验、卡方检验、依据Mann和Whitney的U检验、Kruskal-Wallis检验(H检验)、Jonckheere-Terpstra检验和Wilcoxon检验确定,其在统计学显著数目的患者中应当减轻目标疾病症状。
本披露的复溶溶液可通过任何合适的手段施用,包括肠胃外、肺内和鼻内,并且如果需要局部治疗,则病灶内施用。肠胃外输注包括肌肉内、静脉内、动脉内、腹膜内或皮下施用。给药可以通过任何适当的途径,例如,通过注射,诸如静脉内或皮下注射。本文考虑多种给药时间方案,包括但不限于,单次或在多个时间点多次施用,推注施用和脉冲输注。在一些实施方案中,本披露的复溶溶液通过皮下注射施用。
本披露的复溶溶液将以符合良好医疗实践的方式配制、给药和施用。在此背景下考虑的因素包括所治疗的具体病症、所治疗的具体哺乳动物、个体患者的临床状况、病症的起因、试剂的递送部位、施用方法、施用时间安排以及医学从业者已知的其他因素。任选地,复溶溶液还可以与用于预防或治疗所述病症的一种或更多种其它试剂一起配制。此类其它试剂的有效量取决于复溶溶液中存在的抗原结合分子的量、病症或治疗的类型以及其它因素。可以与本文所述相同的剂量和施用路径使用,或以本文所述剂量的约1至99%使用,或以任何剂量使用,并通过经验/临床确定为合适的任何途径使用。在以上说明书中提出了本披露一种或多种实施方式的细节。虽然可使用与本文所述类似或相同的任何方法和材料来实施或测试本披露,但是以下描述优选的方法和材料。通过说明书和权利要求书,本披露的其他特点、目的和优点将是显而易见的。在说明书和权利要求书中,除
非上下文中有清楚的另外指明,单数形式包括复数指代物的情况。除非另有定义,本文使用的所有技术和科学术语都具有本披露所属领域普通技术人员所理解的一般含义。说明书中引用的所有专利和出版物都通过引用纳入。提出以下实施例是为了更全面地说明本披露的优选实施方式。这些实施例不应以任何方式理解为限制本披露的范围,本披露的范围由权利要求书限定。
实施例-特异性结合HGFR和EGFR的双特异性抗体制备与检测
PCT/CN2022/105714(申请日:2022.07.14;优先权专利申请号:CN202110794137.9)通过援引完整收入本披露。
实施例1:抗原蛋白及抗体的制备
1.1抗原蛋白结构
以人EGFR蛋白(UniProt Epidermal growth factor receptor,Uniprot号:P00533)作为EGFR的模板,在EGFR蛋白基础上融合His标签,设计本发明检测用的带His标签的人EGFR蛋白胞外域(简称:EGFR-His);其氨基酸序列如下:
EGFR-His的氨基酸序列:
注释:划线部分为EGFR蛋白胞外域;斜体部分为His标签。
以人HGFR蛋白(UniProt Hepatocyte growth factor receptor,Uniprot号:P08581)作为HGFR的模板,在HGFR蛋白基础上融合His标签或Fc,设计本发明检测用的带His标签的HGFR蛋白胞外域(简称HGFR-His)、带Fc标签的HGFR蛋白胞外域(简称HGFR-Fc),其氨基酸序列如下:
HGFR-His的氨基酸序列:
注释:划线部分为HGFR蛋白胞外域;斜体部分为His标签。
以人HGF蛋白(UniProt Hepatocyte growth factor,Uniprot号:P14210)作为HGF的模板,设计本披露检测用带His标签的HGF蛋白(简称HGF-His),其氨基酸序列如下:
HGF-His的氨基酸序列:
注释:划线部分为HGF蛋白;斜体部分为His标签。
1.2蛋白的纯化
1.2.1重组蛋白的纯化步骤:
将细胞表达上清样品高速离心去除杂质,并将缓冲液置换为PBS,加入咪唑至终浓度为5mM。用含有5mM咪唑的PBS溶液平衡镍柱,冲洗2-5倍柱体积。将置换后的细胞上清样品上Ni Sepharose excel柱(GE,17-3712-02)。用含有5mM咪唑的PBS溶液冲洗柱子,至A280读数降至基线。后用PBS+10mM咪唑冲洗层析柱,除去非特异结合的杂蛋白,并收集流出液。再用含有300mM咪唑的PBS溶液洗脱目的蛋白,并收集洗脱峰。收集的洗脱液浓缩后用凝胶层析Superdex200(GE,28-9893-35)进一步纯化,流动相为PBS。去除聚体峰,收集洗脱峰。所得到的蛋白经电泳,肽图,LC-MS鉴定为正确后分装备用。
1.2.2抗体的纯化步骤:
将细胞表达的上清样品经高速离心去除杂质,上清进行MabSelect Sure(GE,17-5438-01)亲和层析。MabSelect Sure层析柱先用0.2M NaOH再生,利用纯水冲洗后用PBS平衡柱子,将上清结合后,利用PBS进行洗涤至A280读数降至基线。用0.1M醋酸缓冲液在pH3.5条件下洗脱目的蛋白,用1M Tris-HCl中和。洗脱样品适当浓缩后利用PBS平衡好的凝胶层析Superdex200(GE,28-9893-35)进一步纯化,合并收集目的蛋白所在接收管浓缩至适当浓度。此方法用来纯化本发明中涉及的抗体。
实施例2.特异性结合HGFR和EGFR的双特异性抗体的制备及鉴定
利用特异性结合HGFR的分子与特异性结合EGFR的分子,构建特异性结合HGFR和EGFR的抗体。
2.1特异性结合EGFR的分子
特异性结合EGFR的分子可以来源于任意适宜的抗体,例如Zalutumumab(简称Zal)或其变体(例如Zal.1,Zal.1是通过将Zal轻链第一位氨基酸残基由A突变为D获得),其中:
>Zal重链的氨基酸序列:
>Zal轻链的氨基酸序列:
>Zal的重链可变区的氨基酸序列:
>Zal的轻链可变区的氨基酸序列:
>Zal.1的重链可变区的氨基酸序列为SEQ ID NO:3
>Zal.1的轻链可变区的氨基酸序列:
Zal和Zal.1的CDR序列见表2:
表2.特异性结合EGFR分子的CDR
备注:上述CDR根据Kabat编号***确认。
2.2特异性结合HGFR的分子
特异性结合HGFR的分子可以来源于任意适宜的抗体,包括描述于国际公开号WO2013003680A1(通过援引完整收入本文)中的Onartuzumab(简称Omab)等抗体;描述于国际公开号WO2016165580A1(通过援引完整收入本文)中的Ab10等抗体;以及上述抗体的突变抗体。其中,Omab、Ab10的可变区的氨基酸序列如下:
>Omab的重链可变区的氨基酸序列:
>Omab的轻链可变区的氨基酸序列:
>Ab10的重链可变区的氨基酸序列:
>Ab10的轻链可变区的氨基酸序列:
通过对Ab10的CDR进行突变,获得Ab10突变抗体:Ab10.1;Ab10.1的恒定区与Ab10相同,Ab10.1可变区氨基酸序列如下:
>Ab10.1的重链可变区的氨基酸序列:
>Ab10.1的轻链可变区的氨基酸序列:
备注:上述特异性结合HGFR分子的序列中,其中下划线部分为根据Kabat编号***确认的CDR区部分,斜体加粗部分为突变氨基酸残基。
特异性结合HGFR分子的CDR见表3:
表3.特异性结合HGFR分子的CDR
备注:上述CDR根据Kabat编号***确认。
2.3特异性结合HGFR和EGFR的双特异性抗体
利用特异性结合HGFR的分子与特异性结合EGFR的分子,构建特异性结合HGFR和EGFR的具有Format1或Format2结构的双特异性抗体,Format1的结构示意图见图1,Format2的结构示意图见图2。其中,
Format1包含如下结构的4条链,其中:
链1为:
VH(抗EGFR抗体)-连接子1-VH(抗HGFR抗体1)-IgG1(CH1)-IgG1Fc(knob),
链2为:VL(抗EGFR抗体)-连接子1-VL(抗HGFR抗体1)-CL,
链3为:VH(抗HGFR抗体2)-连接子2-Titin链-IgG1Fc(hole),
链4为:VL(抗HGFR抗体2)-连接子2-Obscurin链。
Format2包含如下结构的4条链,其中:
链1为:VH(抗HGFR抗体)-IgG1(CH1)-IgG1Fc(knob),
链2为:VL(抗HGFR抗体)-CL,
链3为:VH(抗EGFR抗体)-连接子2-Titin链-IgG1Fc(hole),
链4为:VL(抗EGFR抗体)-连接子2-Obscurin链;
示例性地,利用Ab10.1(抗HGFR抗体1)、Omab(抗HGFR抗体2)、Zal.1(抗EGFR抗体)、人IgG1重链恒定区/kappa轻链恒定区以及T.16(Titin链)/O.28(Obscurin链),构建具有Format1结构的EM1和具有Format2结构的EM2。
EM1的4条链的氨基酸序列如下:
EM1的链1的氨基酸序列:
EM1的链2的氨基酸序列:
EM1的链3的氨基酸序列:
EM1的链4的氨基酸序列:
EM2的4条链的氨基酸序列如下所示:
EM2的链1的氨基酸序列:
EM2的链2的氨基酸序列:
EM2的链3的氨基酸序列:
EM2的链4的氨基酸序列:
备注:上述序列中,单下划线部分为可变区,虚下划线部分为CL,点下划线部分为Fc区,斜体部分为CH1,双下划线部分为连接子,波浪线部分为Obscurin链/Titin-T链。
连接子1的氨基酸序列:GGGGSGGGG(SEQ ID NO:45)
连接子2的氨基酸序列:GGGGS(SEQ ID NO:46)
IgG1Fc(knob)的氨基酸序列:
IgG1Fc(hole)的氨基酸序列:
CL:
T.16(Titin链)
O.28(Obscurin链)
测试例
测试例1:Biacore检测体外结合亲和力和动力学实验
通过Biacore T200(GE)测定待测分子与人EGFR(SEQ ID NO:42)或人HGFR蛋白(SEQ ID NO:43)的亲和力,方法如下:
用Protein A生物传感芯片亲和捕获抗体分子,然后于芯片表面流经抗原180秒,之后解离600秒,用Biacore T200仪器实时检测反应信号获得结合和解离曲线。在每个实验循环解离完成后,用10mM Gly-HCl(pH 1.5)将生物传感芯片洗净再生。数据拟合模型采用1:1Model,获得待测分子亲和力数值。本实施例中,
阳性对照MCLA为一种特异性结合HGFR和EGFR的双特异性抗体(具体序列参见WO2019031965A1中的PB8532)。
实验结果见表4,实验结果表明,本披露构建的突变抗体和双特异性抗体均具有很强的亲和力,Ab10.1的亲和力至少是母抗体Ab10亲和力的4倍。
表4:本发明抗体与人EGFR或人HGFR的体外亲和力
测试例2:抗体的体外细胞结合实验
特异性结合HGFR和EGFR的双特异性抗体的细胞结合活性通过流式细胞仪来检测。将1×106个/mL EGFR CHO-S稳转细胞株、HGFR CHO-S稳转细胞株、H1975-HGF或MKN-45肿瘤细胞株用1%BSA PBS缓冲液封闭后,加入不同浓度稀释的抗体样品(C25为阴性对照,其为无关靶点的IgG1抗体蛋白,以下同)孵育1小时。洗两次后,再加入R-PE-羊抗人(H+L)抗体(Invitrogen,CAT#H10104)孵育0.5小时。洗两次后,使用流式细胞仪读取荧光信号值。
实验结果见图3至图6,实验结果显示,本披露的特异性结合HGFR和EGFR的双特异性抗体对肿瘤细胞的结合能力比阳性抗体MCLA更强。
测试例3:抗体拮抗EGFR结合EGF的阻断实验
抗体对EGF/EGFR的阻断能力通过ELISA来检测。包被1μg/mL兔抗His抗体(金斯瑞,A01857),4℃过夜后封闭。洗板后加入2μg/mL EGFR-His(SEQ ID NO:42),孵育1h。洗板后加入2μg/mL EGF-mFc(Acro,EGF-H525b)和不同浓度稀释的抗体,孵育1小时。洗板后加入辣根过氧化物酶-羊抗鼠IgG抗体(Jackson,115-035-062),孵育1小时。洗板后加入四甲基联苯胺溶液显色,最后加入终止液,在酶标仪上测量OD450值。实施例中,阳性对照JNJ为一种特异性结合HGFR和EGFR的双特异性抗体(JNJ也称Amivantamab,具体序列结构参见WHO Drug Information,33(2):237-239)。
实验结果见表5,实验结果表明,本披露的特异性结合HGFR和EGFR的双特异性抗体能有效阻断EGF与EGFR的结合。
表5.双特异性抗原结合分子阻断EGF与EGFR的结合实验结果
测试例4:抗体拮抗HGFR结合HGF的阻断实验
抗体对HGF/HGFR的阻断能力通过ELISA来检测。包被5μg/mL HGFR-His(序列参见SEQ ID NO:43),4℃过夜后封闭。洗板后加入2μg/mL bio-HGF-His(序列参见SEQ ID NO:44)和不同浓度稀释的抗体,孵育1小时。洗板后加入辣根过氧化物酶-链霉亲和素(Jackson,016-030-084),孵育1小时。洗板后加入四甲基联苯胺溶液显色,最后加入终止液,在酶标仪上测量OD450值。
实验结果见表6,实验结果显示,本披露的特异性结合HGFR和EGFR的双特异性抗体阻断HGFR结合HGF的最大阻断力比阳性抗体JNJ更强。
表6.双特异性抗原结合分子阻断HGF与HGFR的结合实验结果
测试例5:抗体对细胞EGFR磷酸化的抑制实验
取H292细胞(也称NCI-H292,人肺癌细胞(***转移),中国科学院细胞库,下同)以12000个/孔铺于96孔板(Corning,3599),培养基为RPMI1640+10%FBS,其后将96孔板在37℃、5%CO2培养箱培养24小时。第二天,弃去培养基,更换成无血清的培养基(RPMI640+25mM HEPES+0.1mM NEAA+1mM丙酮酸钠)饥饿16-20小时。第三天,配制抗体,首浓度为6μM,5倍梯度稀释,更换饥饿培养基,37℃、5%CO2培养箱培养1小时,期间加入50μL100ng/mL rEGF(R&D,236-EG-200),37℃、5%CO2培养箱继续培养15分钟,最后根据PHOSPHO-EGFR(TYR1068)KITS(Cisbio,64EG1PEG)说明书,检测磷酸化EGFR。
实验结果见图7,实验结果显示,本披露的特异性结合HGFR和EGFR的双特异性抗体能有效抑制细胞EGFR磷酸化。
测试例6:抗体对细胞HGFR磷酸化的抑制实验
取H292细胞以12000个/孔铺于96孔板(Corning,3599),培养基为RPMI1640+10%FBS,其后将96孔板在37℃、5%CO2培养箱培养24小时。第二
天,弃去培养基,更换成无血清的培养基(RPMI640+25mM HEPES+0.1mM NEAA+1mM丙酮酸钠)饥饿16-20小时。第三天,配制抗体,首浓度为1μM,10倍梯度稀释,更换饥饿培养基,37℃、5%CO2培养箱培养1小时,期间加入50μL200ng/mL rHGF(R&D,294-HG-005)37℃、5%CO2培养箱继续培养30分钟,最后根据p-c-Met(Tyr1234/1235)Assay Kit(PerkinElmer,ALSU-PCMET-A50)说明书,检测磷酸化HGFR。
实验结果见图8,实验结果显示,本披露的特异性结合HGFR和EGFR的双特异性抗体能有效抑制细胞HGFR磷酸化,抑制能力比Ab10.1强。
测试例7:抗体对细胞AKT磷酸化的抑制实验
取H292细胞以12000个/孔铺于96孔板(Corning,3599),培养基为RPMI1640+10%FBS,其后将96孔板在37℃、5%CO2培养箱培养24小时。第二天,弃去培养基,更换成无血清的培养基(RPMI640+25mM HEPES+0.1mM NEAA+1mM丙酮酸钠)饥饿16-20小时。第三天,配制抗体,首浓度为4μM,5倍梯度稀释,更换饥饿培养基,37℃、5%CO2培养箱培养1小时,期间加入50μL200ng/mL rHGF(R&D,294-HG-005)和50ng/mL rEGF(R&D,236-EG-200),混合均匀,37℃、5%CO2培养箱继续培养1小时,最后根据PHOSPHO-AKT(SER473)KITS(Cisbio,64AKSPEG)说明书,检测磷酸化AKT。细胞内AKT磷酸化水平通过665nm的荧光信号值与620nm的荧光信号值比值(ratio)来反映,比值=665nm处荧光信号值/620nm处荧光信号值*104,比值越高,细胞内AKT磷酸化水平越高。
实验结果见图9,实验结果显示,本披露的特异性结合HGFR和EGFR的双特异性抗体能有效抑制细胞AKT磷酸化,且抑制能力比阳性对照抗体强。
测试例8:抗体降低细胞表面的HGFR表达
取HCC827细胞(人非小细胞肺癌细胞,源自ATCC)以3×105个/孔铺于6孔板,培养基RPMI1640+10%FBS,其后将6孔板在37℃,5%CO2培养箱培养24小时。配制抗体至浓度200nM,处理且反应18小时。处理过后,PBS洗细胞一次,加入500μL无酶细胞解离缓冲液(Gibco,#13151)收集细胞,通过离心分离细胞与无酶细胞解离缓冲液。使用羊抗人HGFR抗体(R&D systems,AF276)进行免疫荧光染色,4℃反应1小时。随后,用含2%FBS的PBS洗两次。其后加入ALEXA488-驴抗羊二抗(Thermo Fisher,A-11055)4℃反应1小时,洗两次后用BD Cytofix(BD,#554655)固定细胞。再用PBS洗一次后,使用流式细胞仪读取荧光信号值。
实验结果见图10,实验结果显示,相较阳性对照抗体,本披露的特异性结合HGFR和EGFR的双特异性抗体能更有效地减少细胞表面的HGFR,抑制受体信号
通路。
测试例9:抗体对SNU-5细胞的增殖抑制实验
取SNU-5细胞(人胃癌细胞,源自ATCC)以4000个/孔铺于96孔板(Corning,3903),培养基为IMDM+5%FBS,其后将96孔板在37℃、5%CO2培养箱培养24小时。配制抗体至浓度5μM,5倍梯度稀释,加入上述96孔板,其后在37℃、5%CO2培养箱培养5天,最后,根据说明书配制Luminescent Cell Viability Assay(Promega,G7573),每孔加入50μL CTG,室温下孵育10min,用封底膜封好后,用多标记微孔板检测***(PerkinElmer,victor3)检测荧光强度(Luminescence,简称Lum)。
计算公式:Lum=测定值-培养基读数;
%抑制=(Lum0-Lum)/Lum0*100%;
其中Lum0为抗体浓度为0的孔的荧光强度,Lum为各稀释浓度抗体孔的荧光强度。
实验结果见图11,实验结果显示,本披露的特异性结合HGFR和EGFR的双特异性抗体对SNU-5细胞的增殖抑制能力比阳性抗体更强。
测试例10:抗体对肿瘤细胞的ADCC杀伤实验
配制抗体至200nM浓度,5倍梯度稀释。分别收集肿瘤细胞:Hs746T细胞(人胃癌细胞,南京科佰生物科技有限公司)和H292细胞,PBS洗一次后,用缓冲液(PBS+10%FBS+20mM HEPES)调整细胞密度为1×106个/mL,然后在2mL细胞中加入3μL BATDA试剂(PerkinElmer,AD0116),在37℃,5%CO2培养箱孵育15min。离心后,用冲洗缓冲液(PBS+20mM HEPES)洗三次,计数调整细胞密度为1×105个/mL。收集NK92--FCGR3A(176V)细胞(稳定转化有FCGR3A(176V)的NK92细胞(购自南京科佰生物科技有限公司)),PBS洗一次后,用培养基重悬细胞计数,调整细胞密度为5×105个/mL。最后在96孔板(corning,3788)中加入100μL NK92--FCGR3A(176V)细胞,50μL稀释抗体,50μL标记后的肿瘤细胞。同时,设置自发对照释放孔,其不含NK92--FCGR3A(176V)细胞,而含有100μL培养基;设置阳性对照孔,其含有10μL裂解缓冲液+50μL标记后的肿瘤细胞+140μL培养基;设置背景对照孔,其含标记后的细胞上清50μL+150μL培养基。然后将96孔板300rpm离心2分钟后,在37℃,5%CO2培养箱孵育2小时。取上清100μL到新96孔板(corning,3788),300g离心5min后,取20μL到检测板(PerkinElmer,AD0116),然后加入200μL铕溶液(PerkinElmer,AD0116),室温下震荡孵育15分钟,最后检测TRF。其中,EM1(afuc)为去岩藻糖基化的EM1;EM2(afuc)为去岩藻糖基化的EM2。
计算公式:特异释放%=(实验释放-自发释放)/(最大释放-自发释放)*100。
实验结果见图12和图13,实验结果显示,本披露的特异性结合HGFR和EGFR的双特异性抗体去岩藻糖基化后,可显著提高其对细胞的ADCC杀伤能力。
测试例11:抗体在小鼠H1975-HGF模型的体内药效实验
将H1975-HGF细胞(稳转人HGF的H1975细胞(人肺腺癌细胞,ATCC))(5×106个)接种于CD1裸雌性小鼠(维通利华)右肋部皮下。
当肿瘤体积大小为189mm3时,根据肿瘤体积大小将动物随机分组,每组10只,包括C25-3mpk组(阴性对照,其为无关靶点的IgG1抗体蛋白,给药剂量为3mg/kg)、JNJ-3mpk组(阳性对照,给药剂量为3mg/kg)和EM1-3.5mpk组(EM1试验组,给药剂量为3.5mg/kg)。以动物分组(0天)起,每周给药两次,腹腔注射给药,连续2到3周。每周测量肿瘤体积2次,称小鼠体重,记录数据。通过T-检验对数据进行统计学分析。其中,C25-3mpk、JNJ-3mpk、EM1-3.5mpk为等摩尔量。
肿瘤体积(T)计算公式:T=1/2长×(短)2;
相对肿瘤增殖率T/C%=(T-T0)/(C-C0)×100%;
抑瘤率TGI%=1-T/C%;
其中,T和T0为给药组肿瘤体积,其中T0为实验开始时的肿瘤体积;C和C0是阴性对照组肿瘤体积,其中C0是阴性对照组实验开始时的肿瘤体积。
实验结果见表7,实验结果显示,本披露的特异性结合HGFR和EGFR的双特异性抗体能显著抑制肿瘤细胞的生长,第21天时,等摩尔的本披露的特异性结合HGFR和EGFR的双特异性抗体组的平均肿瘤体积小于阳性对照的一半,抑瘤药效强于阳性对照抗体JNJ。
表7.抗体在小鼠H1975-HGF模型的体内药效实验
测试例12:抗体在小鼠HCC827模型的体内药效实验
将HCC827细胞(人非小细胞肺癌细胞,源自ATCC)(8.555×106个)接种于NUNU雌性小鼠(维通利华)右肋部皮下。
当瘤体积大小为200mm3时,根据瘤体积大小将动物随机分组,每组10只,包括C25-3mpk组(阴性对照,其中C25为无关靶点的IgG1抗体蛋白,给药剂量为3mg/kg)、MCLA-3mpk组(阳性对照,给药剂量为3mg/kg)、EM1-3.5mpk组(EM1高剂量试验组,给药剂量为3.5mg/kg)、EM1-1.17mpk组(EM1低剂量试验组,给药剂量为1.17mg/kg)和EM2-3mpk组(EM2试验组,给药剂量为3mg/kg)。
以动物分组(0天)起,每周给药两次,腹腔注射给药,连续2到3周。每周测量肿瘤体积2次,称小鼠体重,记录数据。其中,C25-3mpk、MCLA-3mpk、EM1-3.5mpk和EM2-3mpk为等摩尔量。
实验结果见图14,实验结果显示,本披露的特异性结合HGFR和EGFR的双特异性抗体能显著抑制肿瘤细胞的生长,抑瘤效果强于阳性对照抗体。
制备实施例-特异性结合HGFR和EGFR的双特异性抗体制剂
SEC分子排阻色谱法:
根据凝胶孔隙的孔径大小与高分子样品分子的线团尺寸间的相对关系而对溶质进行分离的分析的方法。
SEC%(SEC单体含量百分比)=A单体/A总×100%(A单体为样品中主峰单体的峰面积,A总为所有峰面积之和)。ΔSEC%=稳定性实验后制剂的SEC%-稳定性实验前制剂的SEC%。
SEC测定用仪器:安捷伦HPLC 1260;
柱子:Waters,BioResolveTM SEC mAb2.5μm,7.8×300mm。
NR-CE毛细管凝胶电泳:
将凝胶移到毛细管中作为支持介质进行的一种电泳,并在一定的电压下根据样品分子量的大小进行分离的方法。
NR-CE%=A主峰/A总×100%(A主峰为样品中主峰的峰面积,A总为所有峰面积之和)。
ΔNR-CE%=稳定性实验后制剂的NR-CE%-稳定性实验前制剂的NR-CE%。
CE测定用仪器:Beckman毛细管电泳仪,型号PA800plus。
IEC离子交换色谱:
以离子交换树脂或化学键合离子交换剂为固定相,利用被分离组分离子交换能力的差别或选择性系数的差别而实现分离的色谱方法。
IEC%=A中性峰面积/A总面积×100%(A总面积为酸性峰、中性峰和碱性峰面积之和)。ΔIEC%=稳定性实验后制剂的IEC%-稳定性实验前制剂的IEC%。
IEC测定用仪器:安捷伦HPLC 1260。
渗透压测定:
冰点法测定渗透压,以冰点下降值与溶液的摩尔浓度成正比例关系为基础,采用高灵敏度感温元件,测定溶液结冰点,通过电量转化为渗透压。
渗透压测定用仪器:罗泽Loser,型号OM815。
蛋白
以下实施例所采用的蛋白为HGFR-EGFR双特异性抗体EM1(以下简称“蛋白”)。
蛋白的浓度测定用仪器:紫外可见分光光度计,型号:Nano Drop oneC,光程为1mm。
制剂实施例1.不同离子强度筛选
用表8所示的缓冲体系制备100mg/mL蛋白、80mg/mL蔗糖、0.4mg/mL聚山梨酯80(PS80)的制剂。对样品进行强制降解研究(40℃放置4周),以SEC和NR-CE为评价指标,考察不同缓冲体系对蛋白稳定性的影响。
结果见表8。SEC数据显示,两者没有明显差异。NR-CE数据显示,20mM离子强度的制剂组优于50mM离子强度的制剂组。
表8不同离子强度筛选结果
注:His-HCl代表组氨酸-盐酸组氨酸;40℃ W4:40℃放置4周,下同。
制剂实施例2.pH和缓冲体系筛选
用表9所示的缓冲体系制备100mg/mL蛋白、80mg/mL蔗糖、0.4mg/mL聚山梨酯80(PS80)的制剂。对样品进行强制降解研究(40℃放置4周),以外观、SEC和NR-CE为评价指标,考察不同缓冲体系对蛋白稳定性的影响。
结果见表9。外观数据显示,含His-HCl和His-AA的制剂的外观优于其他组。SEC及NR-CE数据显示,40℃放置4周后,含His-HCl(pH 6.0或pH 6.5)的制剂的单体纯度优于其他组。
表9 pH和缓冲体系筛选结果
注:AA代表醋酸-醋酸钠盐;His-AA代表组氨酸-醋酸组氨酸;CA代表枸橼酸-枸橼酸钠盐;SA代表琥珀酸-琥珀酸钠盐;PB代表磷酸氢二钠-磷酸二氢钠盐;下同。
制剂实施例3.糖种类筛选
用20mM His-HCl缓冲液(pH 6.0)制备100mg/mL蛋白、表10所示的不同种类的糖和0.6mg/mL PS80的制剂。对样品进行强制降解研究(40℃放置4周),以外观、SEC、NR-CE为评价指标,考察不同糖种类对蛋白稳定性的影响。
结果见表10,外观和纯度项数据显示,40℃放置4周后,含蔗糖的制剂组优于含海藻糖的制剂组。
表10糖种类筛选结果
制剂实施例4.表面活性剂筛选
用20mM His-HCl缓冲液(pH 6.0)制备100mg/mL蛋白、75mg/mL蔗糖、表11所示表面活性剂的制剂。对样品进行强制降解研究(25℃,300rpm振摇7天;40℃放置4周),以SEC和NR-CE为评价指标,考察不同表面活性剂对蛋白稳定性的影响。
结果见表11。外观数据显示,振摇7天后,含0.4mg/mL PS80的制剂组外观差于其他制剂组;40℃放置4周后,含0.6mg/mL泊洛沙姆188(P188)的制剂组外观差于其他制剂组。NR-CE数据显示,40℃放置4周后,含0.6mg/mL泊洛沙姆188(P188)的制剂组差于其他制剂组,含0.4mg/mL PS80的制剂组略优,含0.6mg/mL PS80的制剂组和含0.8mg/mL PS80的制剂组没有明显差异。
表11表面活性剂筛选结果
注:振摇D7:振摇7天;40℃ W4:40℃放置4周。
制剂实施例5.冻干
制备表12蛋白样品并进行冻干,冻干参数见表13:
表12蛋白冻干剂处方设计表
表13蛋白冻干工艺开发冻干参数
*:一次干燥与二次干燥时间根据具体情况进行调整。
两种冻干成品外观显示粉饼均完整,无明显塌陷,粉饼外观无显著差异。
制剂实施例6.冻干稳定性1
用10mM His-HCl pH6.0制备37.5mg/mL蔗糖、0.3mg/mL PS80、50mg/mL蛋白样品的制剂,用20mL西林瓶和20mm冻干胶塞进行冻干工艺筛选。冻干参数见表14。终工艺样品冻干前后及高温稳定性见表15。
表14蛋白冻干工艺开发冻干参数
*:一次干燥与二次干燥时间根据具体情况进行调整。
表15冻干后产品稳定性数据
结果显示,冻干前外观澄明,冻干后粉饼完整无明显塌陷,复溶澄明;高温(40℃)结果显示,40℃4W条件下SEC、NR-CE和IEC无显著变化,稳定性较好。
制剂实施例7.粘度与pH关系
分别将用20mM His-HCl pH5.0、20mM His-HCl pH6.0制备的蛋白浓缩至不同浓度,测定其在25℃的粘度。结果见表16,在His-HCl体系中,pH 5.0的粘度低于pH 6.0。
表16粘度与pH的关系
注:N/A表示由于样品粘度太大,无法制备。
制剂实施例8.冻干稳定性2(皮下制剂)
制备表17蛋白样品并进行冻干,冻干参数见表14:
表17蛋白冻干剂处方设计表
表18蛋白复溶处方
表19冻干后产品稳定性数据
结果显示,冻干前外观澄明,冻干后粉饼完整无明显塌陷,复溶澄明;复溶后25℃24h结果显示,SEC、NR-CE和IEC无显著变化,稳定性较好。
制剂实施例9.冻干稳定性3(皮下制剂)
制备表20蛋白样品并进行冻干,冻干参数见表14:
表20蛋白冻干剂处方设计表
表21蛋白复溶处方
表22冻干后产品稳定性数据
结果显示,冻干前外观澄明,冻干后粉饼完整无明显塌陷,复溶澄明;高温结果显示,40℃4W条件下SEC和酶活无显著变化,稳定性较好。
制剂实施例10.冻干稳定性4(皮下制剂)
制备表23蛋白样品并进行冻干,冻干参数见表14:
表23蛋白冻干剂处方设计表
表24蛋白复溶处方
表25冻干后产品稳定性数据
注:40℃4W表示该条件下40℃放置4周,2-8℃M6表示该条件下4℃放置6个月,25℃M6表示该条件下25℃放置6个月。
结果显示,冻干前外观澄明,冻干后粉饼完整无明显塌陷,复溶澄明;40℃4W、25℃M6及2-8℃M6结果显示,纯度项和酶活无显著变化,稳定性较好。
制剂实施例11.终处方冻干5(皮下制剂)
制备表26蛋白样品并进行冻干,冻干参数见表14:
表26蛋白冻干剂处方设计表
冻干结果显示,冻干成品外观显示粉饼完整,无明显塌陷。复溶后处方为蛋白浓度150mg/mL,25mM pH5.0His-HCl,蔗糖70mg/mL,10mM甲硫氨酸,PS800.6mg/mL,2000U/mL透明质酸酶,复溶后外观澄清透明。
Claims (23)
- 一种药物组合物,包含特异性结合HGFR和EGFR的双特异性抗体和缓冲剂,其中:所述特异性结合HGFR和EGFR的双特异性抗体包含至少一个特异性结合HGFR的抗原结合模块1和至少一个特异性结合EGFR的抗原结合模块2;所述抗原结合模块1包含重链可变区M-VH和轻链可变区M-VL,所述M-VH包含M-HCDR1、M-HCDR2和M-HCDR3,所述M-VL包含M-LCDR1、M-LCDR2和M-LCDR3,和所述抗原结合模块2包含重链可变区E-VH和轻链可变区E-VL,所述E-VH包含E-HCDR1、E-HCDR2和E-HCDR3,所述E-VL包含E-LCDR1、E-LCDR2和E-LCDR3,所述E-HCDR1、E-HCDR2、E-HCDR3、E-LCDR1、E-LCDR2和E-LCDR3是根据Kabat编号规则定义的,所述E-HCDR1如SEQ ID NO:6所示,所述E-HCDR2如SEQ ID NO:7所示,所述E-HCDR3如SEQ ID NO:8所示,所述E-LCDR1如SEQ ID NO:9所示,所述E-LCDR2如SEQ ID NO:10所示,和所述E-LCDR3如SEQ ID NO:11所示;和所述M-HCDR1、M-HCDR2、M-HCDR3、M-LCDR1、M-LCDR2和M-LCDR3是根据Kabat编号规则定义的,其中,(i)所述M-HCDR1如SEQ ID NO:30所示,所述M-HCDR2如SEQ ID NO:31所示,所述M-HCDR3如SEQ ID NO:32所示,所述M-LCDR1如SEQ ID NO:27所示,所述M-LCDR2如SEQ ID NO:33所示,和所述M-LCDR3如SEQ ID NO:29所示,或(ii)所述M-HCDR1如SEQ ID NO:18所示,所述M-HCDR2包含SEQ ID NO:19所示,所述M-HCDR3包含SEQ ID NO:20所示,所述M-LCDR1包含SEQ ID NO:21所示,所述M-LCDR2包含SEQ ID NO:22所示,和所述M-LCDR3包含SEQ ID NO:23所示,或(iii)所述M-HCDR1如SEQ ID NO:24所示,所述M-HCDR2如SEQ ID NO:25所示,所述M-HCDR3如SEQ ID NO:26所示,所述M-LCDR1如SEQ ID NO:27所示,所述M-LCDR2如SEQ ID NO:28所示,和所述M-LCDR3如SEQ ID NO:29所示;所述缓冲剂为组氨酸缓冲剂、醋酸盐缓冲剂、枸橼酸盐缓冲剂、琥珀酸盐缓冲剂或磷酸盐缓冲剂;优选地,所述缓冲剂为组氨酸-盐酸组氨酸缓冲剂或组氨酸-醋酸组氨酸缓冲剂。
- 根据权利要求1所述的药物组合物,其中所述药物组合物的pH为4.5至 6.5;优选地,所述药物组合物的pH为4.8至6.2;更优选地,所述药物组合物的pH为5.0至6.0。
- 根据权利要求1或2所述的药物组合物,其中所述特异性结合HGFR和EGFR的双特异性抗体的浓度为1mg/mL至250mg/mL;优选地,所述特异性结合HGFR和EGFR的双特异性抗体的浓度为1mg/mL至200mg/mL;更优选地,所述特异性结合HGFR和EGFR的双特异性抗体的浓度为50mg/mL至200mg/mL;最优选地,所述特异性结合HGFR和EGFR的双特异性抗体的浓度为80mg/mL至180mg/mL。
- 根据权利要求1至3任一项所述的药物组合物,其中所述药物组合物包含表面活性剂;优选地,所述表面活性剂为聚山梨酯或泊洛沙姆;更优选地,所述表面活性剂为聚山梨酯80。
- 根据权利要求4所述的药物组合物,其中所述表面活性剂的浓度为0.01mg/mL至1.0mg/mL;优选地,所述表面活性剂的浓度为0.1mg/mL至0.8mg/mL;更优选地,所述表面活性剂的浓度为0.4mg/mL至0.8mg/mL。
- 根据权利要求1至5任一项所述的药物组合物,其中所述药物组合物包含糖;优选地,所述糖为蔗糖、海藻糖、甘露醇或山梨糖醇;更优选地,所述糖为蔗糖。
- 根据权利要求6所述的药物组合物,其中所述糖的浓度为10mg/mL至100mg/mL;优选地,所述糖的浓度为20mg/mL至80mg/mL;更优选地,所述糖的浓度为60mg/mL至80mg/mL。
- 根据权利要求1至7任一项所述的药物组合物,其中所述缓冲剂的浓度为5mM至100mM;优选地,所述缓冲剂的浓度为5mM至50mM;更优选地,所述缓冲剂的浓度为5mM至30mM。
- 根据权利要求1至8任一项所述的药物组合物,其中所述药物组合物还包含辅料;优选地,所述辅料为甲硫氨酸、盐酸精氨酸、甘氨酸、脯氨酸、组氨酸、苯丙氨酸、谷氨酸、天冬氨酸、氯化钠、氯化钙或乙二胺四乙酸二钠;更优选地,所述辅料为甲硫氨酸或盐酸精氨酸;最优选地,所述辅料为甲硫氨酸。
- 根据权利要求9所述的药物组合物,其中所述辅料的浓度为1mM至300mM;优选地,辅料的浓度为1mM至200mM;更优选地,辅料的浓度为1mM至20mM。
- 根据权利要求1至10任一项所述的药物组合物,其中所述药物组合物还包含酶;优选地,所述酶为透明质酸酶。
- 根据权利要求11所述的药物组合物,其中所述酶的浓度为10U/mL至3000U/mL;优选地,所述酶的浓度为150U/mL至2400U/mL;更优选地,所述酶的浓度为700U/mL至2400U/mL;更优选地,所述酶的浓度为1600U/mL至2400U/mL。
- 根据权利要求1至12任一项所述的药物组合物,其中所述的特异性结合HGFR和EGFR的双特异性抗体中,(i)所述特异性结合HGFR和EGFR的双特异性抗体包含两个特异性结合HGFR的抗原结合模块1和一个特异性结合EGFR的抗原结合模块2,其中一个抗原结合模块1,其M-VH具有:如SEQ ID NO:30所示的M-HCDR1、如SEQ ID NO:31所示的M-HCDR2和如SEQ ID NO:32所示的M-HCDR3,并且其M-VL具有:如SEQ ID NO:27所示的M-LCDR1、如SEQ ID NO:33所示的M-LCDR2和如SEQ ID NO:29所示的M-LCDR3;另一个抗原结合模块1,其M-VH具有:如SEQ ID NO:18所示的M-HCDR1、如SEQ ID NO:19所示的M-HCDR2和如SEQ ID NO:20所示的M-HCDR3,并且其M-VL具有:如SEQ ID NO:21所示的M-LCDR1、如SEQ ID NO:22所示的M-LCDR2和如SEQ ID NO:23所示的M-LCDR3;和所述抗原结合模块2,其E-VH具有:如SEQ ID NO:6所示的E-HCDR1、如SEQ ID NO:7所示的E-HCDR2和如SEQ ID NO:8所示的E-HCDR3,并且其E-VL具有:如SEQ ID NO:9所示的E-LCDR1、如SEQ ID NO:10所示的E-LCDR2和如SEQ ID NO:11所示的E-LCDR3;或(ii)所述特异性结合HGFR和EGFR的双特异性抗体包含一个特异性结合 HGFR的抗原结合模块1和一个特异性结合EGFR的抗原结合模块2,所述抗原结合模块1,其M-VH具有:如SEQ ID NO:18所示的M-HCDR1、如SEQ ID NO:19所示的M-HCDR2和如SEQ ID NO:20所示的M-HCDR3,并且其M-VL具有:如SEQ ID NO:21所示的M-LCDR1、如SEQ ID NO:22所示的M-LCDR2和如SEQ ID NO:23所示的M-LCDR3;和所述抗原结合模块2,其E-VH具有:如SEQ ID NO:6所示的E-HCDR1、如SEQ ID NO:7所示的E-HCDR2和如SEQ ID NO:8所示的E-HCDR3,并且其E-VL具有:如SEQ ID NO:9所示的E-LCDR1、如SEQ ID NO:10所示的E-LCDR2和如SEQ ID NO:11所示的E-LCDR3。
- 根据权利要求13所述的药物组合物,其中所述的特异性结合HGFR和EGFR的双特异性抗体中:(i)所述M-VH包含与SEQ ID NO:16具有至少90%的序列同一性的氨基酸序列,并且所述M-VL包含与SEQ ID NO:17具有至少90%的序列同一性的氨基酸序列;或所述M-VH包含与SEQ ID NO:12具有至少90%的序列同一性的氨基酸序列,并且所述M-VL包含与SEQ ID NO:13具有至少90%的序列同一性的氨基酸序列;或所述M-VH包含与SEQ ID NO:14具有至少90%的序列同一性的氨基酸序列,并且所述M-VL包含与SEQ ID NO:15具有至少90%的序列同一性的氨基酸序列;或(ii)所述E-VH包含与SEQ ID NO:3具有至少90%的序列同一性的氨基酸序列,并且所述E-VL包含与SEQ ID NO:5或SEQ ID NO:4具有至少90%的序列同一性的氨基酸序列;优选地,(i)所述特异性结合HGFR和EGFR的双特异性抗体包含两个特异性结合HGFR的抗原结合模块1和一个特异性结合EGFR的抗原结合模块2,其中一个抗原结合模块1的M-VH如SEQ ID NO:16所示,并且M-VL如SEQ ID NO:17所示;另一个抗原结合模块1的M-VH如SEQ ID NO:12所示,并且M-VL如SEQ ID NO:13所示;和所述抗原结合模块2的E-VH如SEQ ID NO:3所示,并且E-VL如SEQ ID NO:5所示;或(ii)所述特异性结合HGFR和EGFR的双特异性抗体包含一个特异性结合HGFR的抗原结合模块1和一个特异性结合EGFR的抗原结合模块2,所述抗原结合模块1的M-VH如SEQ ID NO:12所示,和所述M-VL如SEQ ID NO:13所示,并且所述抗原结合模块2的E-VH如SEQ ID NO:3所示,和E-VL如SEQ ID NO:5所示。
- 根据权利要求13或14所述的药物组合物,其中所述的特异性结合HGFR和EGFR的双特异性抗体,其具有一条如SEQ ID NO:34所示的第一链、一条如SEQ ID NO:35所示的第二链、一条如SEQ ID NO:36所示的第三链和一条如SEQ ID NO:37所示的第四链;或具有一条如SEQ ID NO:38所示的第一链、一条如SEQ ID NO:39所示的第二链、一条如SEQ ID NO:40所示的第三链和一条如SEQ ID NO:41所示的第四链。
- 根据权利要求1至8、13至15任一项所述的药物组合物,其包含如下组分:(a)1mg/mL至250mg/mL的所述特异性结合HGFR和EGFR的双特异性抗体,(b)0.01mg/mL至1.0mg/mL的表面活性剂,(c)10mg/mL至100mg/mL的糖,和(d)5mM至100mM的缓冲剂,所述药物组合物的pH为4.5至6.5;优选地,所述药物组合物包含如下组分:(a)1mg/mL至200mg/mL的所述特异性结合HGFR和EGFR的双特异性抗体,(b)0.1mg/mL至0.8mg/mL的聚山梨酯80,(c)20mg/mL至80mg/mL的蔗糖,和(d)5mM至50mM的组氨酸缓冲剂;所述药物组合物的pH为4.8至6.2;更优选地,所述药物组合物包含如下组分:(a)80mg/mL至180mg/mL的特异性结合HGFR和EGFR的双特异性抗体,(b)0.4mg/mL至0.8mg/mL的聚山梨酯80,(c)60mg/mL至80mg/mL的蔗糖,和(d)5mM至30mM的组氨酸-盐酸组氨酸缓冲剂或组氨酸-醋酸组氨酸缓冲剂;所述药物组合物的pH为5.0至6.0。
- 根据权利要求1至15任一项所述的药物组合物,其包含如下组分:(a)1mg/mL至250mg/mL的所述特异性结合HGFR和EGFR的双特异性抗体,(b)0.01mg/mL至1.0mg/mL的表面活性剂,(c)10mg/mL至100mg/mL的糖,(d)1mM至300mM的辅料,(e)10U/mL至3000U/mL的酶,和(f)5mM至100mM的缓冲剂,所述药物组合物的pH为4.5至6.5;优选地,所述药物组合物包含如下组分:(a)1mg/mL至200mg/mL的所述特异性结合HGFR和EGFR的双特异性抗体,(b)0.1mg/mL至0.8mg/mL的聚山梨酯80,(c)20mg/mL至80mg/mL的蔗糖,(d)1mM至200mM的甲硫氨酸,(e)700U/mL至2400U/mL的透明质酸酶,和(f)5mM至50mM的组氨酸缓冲剂;所述药物组合物的pH为4.8至6.2;更优选地,所述药物组合物包含如下组分:(a)80mg/mL至180mg/mL的特异性结合HGFR和EGFR的双特异性抗体,(b)0.4mg/mL至0.8mg/mL的聚山梨酯80,(c)60mg/mL至80mg/mL的蔗糖,(d)1mM至20mM的甲硫氨酸,(e)1600U/mL至2400U/mL的透明质酸酶,和(f)5mM至30mM的组氨酸-盐酸组氨酸缓冲剂或组氨酸-醋酸组氨酸缓冲剂;所述药物组合物的pH为5.0至6.0。
- 一种冻干制剂,所述冻干制剂复溶后可形成权利要求1至17任一项所述的药物组合物。
- 一种制备冻干制剂的方法,其中包括将权利要求1至17中任一项所述的药物组合物进行冷冻干燥的步骤。
- 一种冻干制剂,所述制剂通过权利要求19所述的方法获得的。
- 一种复溶溶液,所述复溶溶液通过将权利要求18或20所述的冻干制剂经复溶制备获得。
- 根据权利要求1至17任一项所述的药物组合物或权利要求21所述的复溶溶液,其为静脉注射制剂、皮下注射制剂、腹腔注射制剂或肌肉注射制剂;优选为静脉注射制剂或皮下注射制剂。
- 一种治疗或预防疾病的方法,所述方法包括给予受试者治疗有效量的权利 要求1至17中任一项所述的药物组合物,或权利要求18或20所述的冻干制剂、或如权利要求21所述的复溶溶液;优选地,所述的疾病是肿瘤;更优选地,所述肿瘤选自肺癌、乳腺癌、胰腺癌、结直肠癌、肉瘤、肾细胞癌、肝细胞癌、胃癌、卵巢癌、膀胱癌、头颈癌和胶质母细胞瘤。
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202211485154 | 2022-11-24 | ||
CN202211485154.5 | 2022-11-24 |
Publications (1)
Publication Number | Publication Date |
---|---|
WO2024109914A1 true WO2024109914A1 (zh) | 2024-05-30 |
Family
ID=91195194
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/CN2023/133895 WO2024109914A1 (zh) | 2022-11-24 | 2023-11-24 | 一种包含特异性结合hgfr和egfr的双特异性抗体的药物组合物 |
Country Status (1)
Country | Link |
---|---|
WO (1) | WO2024109914A1 (zh) |
Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102361883A (zh) * | 2009-04-07 | 2012-02-22 | 罗氏格黎卡特股份公司 | 双特异性抗-ErbB-1/抗-c-Met抗体 |
CN104955838A (zh) * | 2012-11-21 | 2015-09-30 | 詹森生物科技公司 | 双特异性EGFR/c-Met抗体 |
CN106188293A (zh) * | 2015-04-17 | 2016-12-07 | 江苏恒瑞医药股份有限公司 | 抗c-Met抗体和抗c-Met抗体-细胞毒性药物偶联物及其医药用途 |
CN110028584A (zh) * | 2018-01-12 | 2019-07-19 | 北京科昕生物科技有限公司 | 针对egfr蛋白和met蛋白的双特异性抗体 |
CN112513074A (zh) * | 2018-06-22 | 2021-03-16 | 健玛保 | 生产两种或更多种不同抗体的受控混合物的方法 |
WO2023284829A1 (zh) * | 2021-07-14 | 2023-01-19 | 江苏恒瑞医药股份有限公司 | 特异性结合hgfr和egfr的抗原结合分子及其医药用途 |
-
2023
- 2023-11-24 WO PCT/CN2023/133895 patent/WO2024109914A1/zh unknown
Patent Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102361883A (zh) * | 2009-04-07 | 2012-02-22 | 罗氏格黎卡特股份公司 | 双特异性抗-ErbB-1/抗-c-Met抗体 |
CN104955838A (zh) * | 2012-11-21 | 2015-09-30 | 詹森生物科技公司 | 双特异性EGFR/c-Met抗体 |
CN106188293A (zh) * | 2015-04-17 | 2016-12-07 | 江苏恒瑞医药股份有限公司 | 抗c-Met抗体和抗c-Met抗体-细胞毒性药物偶联物及其医药用途 |
CN110028584A (zh) * | 2018-01-12 | 2019-07-19 | 北京科昕生物科技有限公司 | 针对egfr蛋白和met蛋白的双特异性抗体 |
CN112513074A (zh) * | 2018-06-22 | 2021-03-16 | 健玛保 | 生产两种或更多种不同抗体的受控混合物的方法 |
WO2023284829A1 (zh) * | 2021-07-14 | 2023-01-19 | 江苏恒瑞医药股份有限公司 | 特异性结合hgfr和egfr的抗原结合分子及其医药用途 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN111744007B (zh) | 一种抗tigit抗体药物组合物及其用途 | |
JP7436477B2 (ja) | TGF-β受容体融合タンパク質医薬組成物およびその使用 | |
TWI761869B (zh) | 包含抗cd47/pd-l1雙特異性抗體的製劑及其製備方法和用途 | |
EA031069B1 (ru) | АНТИТЕЛА ПРОТИВ ИНТЕГРИНОВ αVβ6 И ИХ ПРИМЕНЕНИЕ ДЛЯ ЛЕЧЕНИЯ ЗЛОКАЧЕСТВЕННЫХ НОВООБРАЗОВАНИЙ | |
JP2010505426A (ja) | 非機能性p2x7受容体に対する抗体を産生するハイブリドーマ | |
WO2022068914A1 (zh) | 一种含抗体药物偶联物的药物组合物及其用途 | |
WO2019120269A1 (zh) | Lag-3抗体药物组合物及其用途 | |
CN112203695A (zh) | 用基于铂的试剂和抗组织因子抗体-药物偶联物的组合治疗癌症的方法 | |
CN110960490A (zh) | 一种抗egfr抗体偶联药物组合物及其用途 | |
TW202021620A (zh) | 一種cd40抗體藥物組合物及其用途 | |
WO2024109914A1 (zh) | 一种包含特异性结合hgfr和egfr的双特异性抗体的药物组合物 | |
WO2022237647A1 (zh) | 针对dll3的结合分子及其应用 | |
WO2022068894A1 (zh) | 同时靶向pd-l1和vegf的双功能分子及其医药用途 | |
TW202330027A (zh) | 抗cd-47抗體製劑 | |
WO2023134771A1 (zh) | 抗ctla-4抗体药物组合物及其用途 | |
WO2023040935A1 (zh) | 一种含抗pvrig/tigit双特异性抗体的药物组合物 | |
WO2023185732A1 (zh) | 包含抗Claudin18.2和CD3双特异性抗体的制剂及其制备方法和用途 | |
WO2024027824A1 (zh) | 抗cd112r抗体药物组合物及其用途 | |
WO2023151613A1 (zh) | 一种双特异性抗原结合分子及其应用 | |
CN114316046A (zh) | 一种稳定的抗体组合物 | |
CN116459335A (zh) | 抗cldn-18.2抗体药物组合物及其用途 | |
CN116172947A (zh) | 一种含特异性结合vegf和ang2的双特异性抗体的药物组合物 | |
CN115594762A (zh) | 一种铁蛋白重链抗体及其用途 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
121 | Ep: the epo has been informed by wipo that ep was designated in this application |
Ref document number: 23894003 Country of ref document: EP Kind code of ref document: A1 |