WO2023272797A1 - Procédé d'extraction d'huile essentielle d'alpinia oxyphylla miq pour inhiber listeria monocytogenes, et huile essentielle d'alpinia oxyphylla miq - Google Patents

Procédé d'extraction d'huile essentielle d'alpinia oxyphylla miq pour inhiber listeria monocytogenes, et huile essentielle d'alpinia oxyphylla miq Download PDF

Info

Publication number
WO2023272797A1
WO2023272797A1 PCT/CN2021/106837 CN2021106837W WO2023272797A1 WO 2023272797 A1 WO2023272797 A1 WO 2023272797A1 CN 2021106837 W CN2021106837 W CN 2021106837W WO 2023272797 A1 WO2023272797 A1 WO 2023272797A1
Authority
WO
WIPO (PCT)
Prior art keywords
essential oil
powder
water
listeria monocytogenes
alpinia oxyphylla
Prior art date
Application number
PCT/CN2021/106837
Other languages
English (en)
Chinese (zh)
Inventor
于福来
胡璇
王丹
陈悦
王凯
谢小丽
Original Assignee
中国热带农业科学院热带作物品种资源研究所
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 中国热带农业科学院热带作物品种资源研究所 filed Critical 中国热带农业科学院热带作物品种资源研究所
Priority to JP2021560525A priority Critical patent/JP7507785B2/ja
Publication of WO2023272797A1 publication Critical patent/WO2023272797A1/fr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
    • C11B9/00—Essential oils; Perfumes
    • C11B9/02—Recovery or refining of essential oils from raw materials
    • C11B9/027—Recovery of volatiles by distillation or stripping
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/40—Liliopsida [monocotyledons]
    • A01N65/48—Zingiberaceae [Ginger family], e.g. ginger or galangal
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
    • C11B9/00—Essential oils; Perfumes
    • C11B9/02—Recovery or refining of essential oils from raw materials
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Definitions

  • the invention belongs to the technical field of biomedicine, and in particular relates to a method for extracting a nootropic essential oil inhibiting Listeria monocytogenes and the nootropic essential oil.
  • Listeria monocytogenes (Listeria monocytogenes) is a zoonotic pathogen, it widely exists in nature, food-borne Listeria monocytogenes is harmful to human safety, the bacteria in the environment of 4 °C It can still grow and reproduce, and it is one of the main pathogenic bacteria that threaten human health in refrigerated food.
  • the bacteria has strong resistance to physical and chemical factors, can survive for a long time in different environments such as soil, feces, and silage feed, has strong resistance to alkali and salt, is not easy to be frozen and thawed, can withstand high osmotic pressure, and is not easy to suppressed or killed.
  • the applicant's research found that the nootropic essential oil extracted by a specific method showed obvious inhibitory effect on Listeria monocytogenes.
  • the present invention discloses a method for extracting intellectual essential oil that inhibits Listeria monocytogenes, which is of great significance for the popularization and application of intellectual essential oil.
  • the object of the present invention is to provide a method for extracting the nootropic essential oil that inhibits Listeria monocytogenes and the nootropic essential oil.
  • the present invention scheme comprises the following contents:
  • a kind of extracting method of the intelligence-inducing essential oil that suppresses Listeria monocytogenes comprises the following steps:
  • the powder obtained by crushing and sieving is first placed at 35 ⁇ 5° C. under the condition of relative humidity of 60-70%, and then soaked in water and steam distilled.
  • the standing time is 5-7d.
  • the sieve is a 40-60 mesh sieve.
  • the pH of the water is alkaline.
  • the pH of the water is 7.5-8.5.
  • the pH of the water is 8.5.
  • the temperature of steam distillation is 220-230°C.
  • the present invention provides a nootropic essential oil, which is prepared by the method provided by the present invention and exhibits inhibitory activity against Listeria monocytogenes.
  • the invention provides a method for extracting intelligence-inducing essential oil inhibiting Listeria monocytogenes.
  • the essential oil can be extracted efficiently through a steam distillation method, and the extraction rate can reach more than 1.5%.
  • the extraction method of the invention is easy to operate, does not need to use a large amount of chemical reagents, and is safe and efficient.
  • the nootropic essential oil obtained in the invention exhibits extremely strong inhibitory activity against Listeria monocytogenes.
  • the diameter of the inhibition zone was above 8.6 mm, and the MIC was below 15 ⁇ L/mL.
  • the said nootropic is the dried ripe fruit of the nootropic Alpinia oxyphylla Miq., and the samples were collected in Yongchu Village, Baisha County, Hainan province.
  • the results show that: when the soaking time is 0-2 hours, the soaking time and the extraction rate of the essential oil increase in a positive correlation. The greater the effect.
  • the immersion time is 2-5h, the immersion time and the extraction rate of the nootropic essential oil are negatively correlated, and the oil yield of the nootropic fruit essential oil is greatly lost.
  • the extraction rate of essential oil is reduced, so the soaking time is selected to be 2 to 3 hours.
  • the final conditions for the essential oil of the fruit of the fruit were determined as follows: the degree of crushing was 40 mesh, the ratio of solid to liquid was 1:10, soaked at 230°C for 2.4h and distilled for 5.5h; the extraction rate reached 1.8%.
  • essential oil 1 ⁇ 5 Take the dried and mature fruit of Noutropia chinensis, crush it through a 40-mesh sieve to get the powder; weigh 50g of the powder, and soak it in 10 times the amount of distilled water with pH 7.0, 7.5, 8.0, 8.5, and 9.0 respectively In, soak for 2.4h, steam distillation at 230°C for 5.5h.
  • Essential oil 6 Take the dried and mature fruit of Yizhi, crush it through a 40-mesh sieve to get a powder; weigh 50g of the powder, and soak it in 10 times the amount of distilled water with pH 7.0, soak for 2.4h, and steam distill at 180°C for 5.5h .
  • Essential oils 7 ⁇ 10 Take the dried and mature fruits of Nootropics, crush them through a 40-mesh sieve to obtain powder; place the powder at 35 ⁇ 5°C and 65 ⁇ 5% relative humidity (thickness 2-3cm) 3, 5, 7, 9d; Weigh 50g of the powder, soak it in 10 times the amount of distilled water with pH 8.5, soak for 2.4h, and steam distill at 180°C for 5.5h.
  • strains and Listeria monocytogenes ATCC 19111 and Staphylococcus aureus ATCC 6538 were purchased from Beijing Beina Chuanglian Biotechnology Research Institute.
  • bacterial solution bacterial solution concentration 10 7 cfu/ml, coating amount 60 ⁇ L
  • make a mark and repeat each bacterium three times.
  • the negative control is sterile water
  • the positive control is sterile water.
  • the control was levofloxacin (concentration 15 ⁇ g/ml), and the culture condition was 24 hours in a constant temperature incubator at 37°C.
  • the result standard is: when the diameter of the antibacterial zone is greater than 20mm, it is extremely sensitive; when the diameter is between 15-20mm, it is high-sensitivity; Mei et al., 2018).
  • the MIC test was carried out on a 96-well plate using the micro-doubling dilution method.
  • A1 was used as the first well starting from left to right, and 1 to 12 wells on each plate were liquid medicines, and 3 wells were prepared on each plate.
  • the culture condition is to cultivate in a constant temperature incubator at 37°C for 24 hours, and then use a microplate reader to measure the light absorption value of the fungus.
  • the sample concentration at which the bacteriostatic rate reaches 80% is the minimum inhibitory concentration of the sample to the bacterium. (MIC).

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Environmental Sciences (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Plant Pathology (AREA)
  • Dentistry (AREA)
  • Agronomy & Crop Science (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Mycology (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Organic Chemistry (AREA)
  • Medicines Containing Plant Substances (AREA)
  • Fats And Perfumes (AREA)
  • Food Preservation Except Freezing, Refrigeration, And Drying (AREA)

Abstract

L'invention concerne un procédé d'extraction d'huile essentielle d'Alpinia oxyphylla Miq pour inhiber Listeria monocytogenes, et une huile essentielle d'Alpinia oxyphylla Miq. Le procédé comprend les étapes suivantes : le prélèvement d'Alpinia oxyphylla Miq, le séchage de fruits mûrs, le broyage de ceux-ci et le tamisage pour obtenir une poudre; et le mélange de la poudre avec de l'eau selon un rapport massique matière-liquide de 1:10-12, le repos et le trempage pendant 2 à 3 h, puis la réalisation d'une distillation à la vapeur à 220 à 300 °C pendant 5 à 7 h. L'extraction à haut rendement de l'huile essentielle est obtenue par un procédé de distillation à la vapeur, et un taux d'extraction atteint 1,5 % ou plus. L'huile essentielle d'Alpinia oxyphylla Miq obtenue présente une activité inhibitrice extrêmement forte sur Listeria monocytogenes. Le diamètre de la zone d'inhibition atteint 8,6 Mm ou plus, et la CMI atteint 15 pL/mL ou moins.
PCT/CN2021/106837 2021-06-29 2021-07-16 Procédé d'extraction d'huile essentielle d'alpinia oxyphylla miq pour inhiber listeria monocytogenes, et huile essentielle d'alpinia oxyphylla miq WO2023272797A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2021560525A JP7507785B2 (ja) 2021-06-29 2021-07-16 リステリア・モノサイトゲネスを阻害するヤクチ精油の抽出方法及びヤクチ精油

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CN202110731159.0A CN113416608B (zh) 2021-06-29 2021-06-29 一种抑制单增李斯特菌的益智精油的提取方法及益智精油
CN202110731159.0 2021-06-29

Publications (1)

Publication Number Publication Date
WO2023272797A1 true WO2023272797A1 (fr) 2023-01-05

Family

ID=77717401

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/CN2021/106837 WO2023272797A1 (fr) 2021-06-29 2021-07-16 Procédé d'extraction d'huile essentielle d'alpinia oxyphylla miq pour inhiber listeria monocytogenes, et huile essentielle d'alpinia oxyphylla miq

Country Status (3)

Country Link
JP (1) JP7507785B2 (fr)
CN (1) CN113416608B (fr)
WO (1) WO2023272797A1 (fr)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115141682A (zh) * 2022-06-21 2022-10-04 中国热带农业科学院热带作物品种资源研究所 一种益智仁中精油的提取方法和应用
CN115944069A (zh) * 2022-06-21 2023-04-11 中国热带农业科学院热带作物品种资源研究所 一种益智精油调味料及其制备方法和应用
CN115746957A (zh) * 2022-11-21 2023-03-07 中国热带农业科学院热带作物品种资源研究所 一种诺丽精油在抑制奇异变形杆菌中的应用

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102688163A (zh) * 2012-06-13 2012-09-26 中国医学科学院药用植物研究所海南分所 一种抗皮肤衰老的护肤复方精油
CN105983101A (zh) * 2015-02-03 2016-10-05 中国医学科学院药用植物研究所海南分所 益智精油作为促透剂在药物透皮传输和化妆品领域的应用

Family Cites Families (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1459253A (zh) * 2002-05-22 2003-12-03 殷惠军 一种益智保肝饮品及制备方法
EP1547581A1 (fr) * 2003-12-23 2005-06-29 Vectron Therapeutics AG Vaccin liposomal pour le traitement de maladies malignes humaines et hematologiques
JP4781842B2 (ja) 2006-02-23 2011-09-28 株式会社 資生堂 植物性美白剤の製造方法、植物性美白剤および美白用皮膚外用剤
KR101483440B1 (ko) * 2008-05-02 2015-01-19 (주)아모레퍼시픽 포제를 활용한 약용식물 추출물 및 이를 함유하는 피부외용제 조성물
DE202009004878U1 (de) * 2009-06-03 2009-09-03 Engels, Manfred Ingwer-Zusammensetzungen
WO2013054959A1 (fr) * 2011-10-13 2013-04-18 덕성여자대학교 산학협력단 Composition antibactérienne extraite de la fleur de chrysanthemum morifolium
CN102416133A (zh) * 2011-11-22 2012-04-18 四川新绿色药业科技发展股份有限公司 盐益智仁配方颗粒及其制备方法
JP6258687B2 (ja) 2013-03-21 2018-01-10 白井松新薬株式会社 メイラード反応抑制剤
CN103861003A (zh) * 2014-03-18 2014-06-18 海南医学院 一种治疗肾病的益智-乌药提取物及其制备方法
CN103980380B (zh) * 2014-05-20 2016-08-24 河南中烟工业有限责任公司 益智仁多糖、提取纯化方法及其作为烟草保润剂的应用
CN104073359A (zh) * 2014-06-16 2014-10-01 南京泽朗医药科技有限公司 一种生物酶-超临界流体法提取益智挥发油的工艺
CN104073349A (zh) * 2014-06-16 2014-10-01 南京泽朗医药科技有限公司 一种益智挥发油的提取方法
CN105012826B (zh) * 2015-07-20 2018-09-21 河南大学 一种益智叶提取物及其制备方法、应用
CN105749104A (zh) * 2016-04-05 2016-07-13 王捷 益智中药制剂及其制备方法
JP7033789B2 (ja) * 2016-06-29 2022-03-11 オトノミー，インク． トリグリセリド耳用製剤とその使用
CN106359567A (zh) * 2016-08-29 2017-02-01 王开 一种含有植物精油的水果保鲜剂及应用
CN106138955A (zh) * 2016-09-21 2016-11-23 河南中医药大学 益智仁总黄酮在制备治疗绝经前综合征药物中的应用
WO2018090380A1 (fr) * 2016-11-21 2018-05-24 内蒙古天奇中蒙制药股份有限公司 Capsule et procédé de préparation associé
CN107307262A (zh) * 2017-08-14 2017-11-03 遵义医学院 一种具有抑菌活性的四季桔精油及其制备方法及应用
CN108669280B (zh) * 2018-04-27 2021-10-26 华南师范大学 一种春砂仁与益智仁压片糖果及其制作方法
CN109294743A (zh) * 2018-10-16 2019-02-01 珠海横琴新区御峰沉香产业有限公司 一种提高沉香精油得率的加工方法
CN109925466A (zh) * 2019-04-01 2019-06-25 吉林省浦生泰生物技术有限责任公司 一种调节男士腹泻的肠道微生态制剂及其制备方法
CN110200087A (zh) * 2019-07-05 2019-09-06 中资国业牡丹产业集团有限公司 一种益智型牡丹籽调和油及其制备方法
CN110585385B (zh) * 2019-10-17 2021-08-17 郑州大学第一附属医院 一种益智仁倍半萜类化合物的提取方法和定性分析方法
CN111321034A (zh) * 2020-03-13 2020-06-23 宜宾学院 一种艳山姜精油的提取方法
CN112813111B (zh) * 2021-01-05 2023-06-09 福建龙植生物科技有限公司 微发酵技术制备抗氧化功能性生姜粉的方法
CN112545926A (zh) * 2021-01-27 2021-03-26 安徽大学绿色产业创新研究院 一种艾叶挥发油和艾叶总黄酮的综合提取方法及艾叶挥发油的抗氧化性评价

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102688163A (zh) * 2012-06-13 2012-09-26 中国医学科学院药用植物研究所海南分所 一种抗皮肤衰老的护肤复方精油
CN105983101A (zh) * 2015-02-03 2016-10-05 中国医学科学院药用植物研究所海南分所 益智精油作为促透剂在药物透皮传输和化妆品领域的应用

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
HONGZHENG ZHAO, LUO JIAOYANG, KONG WEIJUN, XU DAVID, ZHAO XIANGSHENG, YANG SHIHAI, YANG MEIHUA: "Study on the Chemical Constituents and Skin-penetration-promoting Effect of the Volatile Oil of Yizhi Leaf", ZHONGYAOCAI - JOURNAL OF CHINESE MEDICINAL MATERIALS, GUOJIA YIYAO GUANLIJU, CN, vol. 40, no. 12, 1 December 2017 (2017-12-01), CN , pages 2864 - 2869, XP093018705, ISSN: 1001-4454, DOI: 10.13863/j.issn1001-4454.2017.12.027 *
HOU JIE, WANG YONG; LI YONG-HUI; GAO BING-MIAO; ZHANG JUN-QING; LUO YU-CHAO: "Comparison of Volatile Oil in Alpinia oxyphylla Extracted by Three Methods", GUANGZHOU CHEMICAL INDUSTRY, vol. 44, no. 15, 1 August 2016 (2016-08-01), pages 1 - 3,6, XP093018693, ISSN: 1001-9677 *
LUO QIN, LI XING, TAN RUI, LI YANG: "Study on optimizing the process of steam distillation for extraction volatile oil from Alpinia oxyphylla and its antimicrobial activities in vitro", HUAXI YAOXUE ZAZHI - WEST CHINA JOURNAL OF PHARMACEUTICALSCIENCES, ZHONGGUO YAOXUEHUI, SICHUAN FENHUI, CHENGDU, CN, vol. 26, no. 2, 1 January 2011 (2011-01-01), CN , pages 147 - 149, XP093018700, ISSN: 1006-0103, DOI: 10.13375/j.cnki.wcjps.2011.02.015 *
WU DAN, CHEN KUN, XU MEI, SONG GUOXIN, HU YAOMING, CHENG HEFA: "Rapid analysis of essential oils in fruits of Alpinia oxyphylla Miq. by microwave distillation and simultaneous headspace solid-phase microextraction coupled with gas chromatography-mass spectrometry", ANALYTICAL METHODS, ROYAL SOCIETY OF CHEMISTRY, GB, vol. 6, no. 24, 21 December 2014 (2014-12-21), GB , pages 9718 - 9724, XP093018708, ISSN: 1759-9660, DOI: 10.1039/C4AY02156H *
YAN XIAO-XIA, QIU YAN-LIAN; WANG MAO-YUAN; WANG QING-LONG; WANG ZHU-NIAN;: "GC-MS Analysis of the Components in Volatile Oils from Alpinia oxyphylla Miq. Fruits Before and After Drying", CHINA CONDIMENT, vol. 45, no. 12, 1 December 2020 (2020-12-01), pages 138 - 141, XP093018696, ISSN: 1000-9973, DOI: 10.3969/j.issn.1000-9973.2020.12.029 *

Also Published As

Publication number Publication date
JP2023547742A (ja) 2023-11-14
CN113416608A (zh) 2021-09-21
CN113416608B (zh) 2023-04-28
JP7507785B2 (ja) 2024-06-28

Similar Documents

Publication Publication Date Title
WO2023272797A1 (fr) Procédé d'extraction d'huile essentielle d'alpinia oxyphylla miq pour inhiber listeria monocytogenes, et huile essentielle d'alpinia oxyphylla miq
Weir The ecology of Zymomonas: a review
CN105861333B (zh) 冠突散囊菌ls1菌株
Chen et al. Sweet sorghum stalks extract has antimicrobial activity
CN111012817A (zh) 一种山茱萸籽提取物的制备方法及应用
CN117158440A (zh) 姜黄碳纳米点在防治小麦赤霉病中的应用
CN113913245B (zh) 一种抑制热带假丝酵母菌的草果精油的提取方法及草果精油
Asghar et al. Assessment of Phytochemical Analysis, Nutritional Composition and Antimicrobial Activity of Moringa oleifera.
Fatoni et al. CuO Nanoparticles: Biosynthesis, characterization and in vitro study
Javeria et al. Parmotrema nilgherrense: potential antimicrobial activity against drug resistant pathogens
Nnaji et al. Assessment of Garcinia kola seed as a natural material for water treatment
Natarajan et al. Occurrence of fungal contaminants and aflatoxin producing potential of Aspergills flavus strains from Indian senna (Cassia angustifolia)
Ulahannan et al. Antibacterial action of leaves of Aegle marmelos
CN112725222B (zh) 一株产复合酶枯草芽孢杆菌q3、培养方法及应用
Li et al. Study on the antibacterial activity of dandelion leaf tea
CN115251094B (zh) 食品包装用箬叶抑菌活性提取物的提取方法
Nyegue et al. Study of Chemical composition, growth inhibition and antigerminative effect of three essential oils from Cameroon on four Bacillus strains
Azemi et al. Anthocyanins from Flower Extract of Clitoria ternatea to Attenuate Food-Borne Pathogen (Escherichia coli and Staphylococcus aureus) for Potential Application as Natural Food Preservative
Guerra et al. Sugarcane filter cake waste as natural antibacterial agent against Xanthomonas citri subsp citri
Poutoum et al. Evaluation of the Efficacy of Albizia zygia Extracts on Bacterial Inhibition in Aquatic Microcosm
CN107929299A (zh) 一种从美洲大蠊提取物中分离获得的二氢异香豆素葡萄糖苷化合物的抗菌用途
Zhang et al. Optimizing the Production of Antioxidant and Antibacterial Alkaloids from Macleaya cordata by Subcritical Water Extraction Technology
Norajit et al. Antimicrobial activities of white, red, and extruded ginsengs with different extraction conditions
Hernandez-Escamilla et al. Phytochemical Alternative for Control of the Erwinia amylovora: Extraction Conditions and Phytochemical Source Selection
Ukaoma1ö et al. Anti–Microbial Activity of Extract of Ocimum Gratissimum Leaf on Four Bacteria Strain in Owerri West Local Government Area of Imo State

Legal Events

Date Code Title Description
ENP Entry into the national phase

Ref document number: 2021560525

Country of ref document: JP

Kind code of ref document: A

121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 21947740

Country of ref document: EP

Kind code of ref document: A1

NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 21947740

Country of ref document: EP

Kind code of ref document: A1