WO2013028912A3 - Procédés de production de multiples polypeptides recombinants dans une cellule hôte fongique filamenteuse - Google Patents

Procédés de production de multiples polypeptides recombinants dans une cellule hôte fongique filamenteuse Download PDF

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Publication number
WO2013028912A3
WO2013028912A3 PCT/US2012/052143 US2012052143W WO2013028912A3 WO 2013028912 A3 WO2013028912 A3 WO 2013028912A3 US 2012052143 W US2012052143 W US 2012052143W WO 2013028912 A3 WO2013028912 A3 WO 2013028912A3
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WO
WIPO (PCT)
Prior art keywords
filamentous fungal
recombinant polypeptides
methods
multiple recombinant
host cell
Prior art date
Application number
PCT/US2012/052143
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English (en)
Other versions
WO2013028912A2 (fr
Inventor
Debbie Yaver
Qiming Jin
Original Assignee
Novozymes, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes, Inc. filed Critical Novozymes, Inc.
Priority to AU2012298799A priority Critical patent/AU2012298799B2/en
Priority to EP12801652.4A priority patent/EP2748322A2/fr
Priority to CN201280052277.8A priority patent/CN103890180A/zh
Priority to US14/238,691 priority patent/US9493790B2/en
Priority to BR112014004190A priority patent/BR112014004190A2/pt
Priority to IN2136CHN2014 priority patent/IN2014CN02136A/en
Publication of WO2013028912A2 publication Critical patent/WO2013028912A2/fr
Publication of WO2013028912A3 publication Critical patent/WO2013028912A3/fr
Priority to US15/280,706 priority patent/US10301610B2/en
Priority to US16/394,951 priority patent/US10822601B2/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01091—Cellulose 1,4-beta-cellobiosidase (3.2.1.91)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/0115—Oligoxyloglucan reducing-end-specific cellobiohydrolase (3.2.1.150)

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Mycology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

La présente invention concerne des procédés de génération d'une souche fongique filamenteuse en vue de la production de multiples polypeptides recombinants ayant une activité biologique. La présente invention concerne également des procédés de production de multiples polypeptides recombinants ayant une activité biologique dans une souche fongique filamenteuse. La présente invention concerne, par ailleurs, des souches fongiques filamenteuses exprimant de multiples polypeptides recombinants ayant une activité biologique.
PCT/US2012/052143 2011-08-24 2012-08-23 Procédés de production de multiples polypeptides recombinants dans une cellule hôte fongique filamenteuse WO2013028912A2 (fr)

Priority Applications (8)

Application Number Priority Date Filing Date Title
AU2012298799A AU2012298799B2 (en) 2011-08-24 2012-08-23 Methods for producing multiple recombinant polypeptides in a filamentous fungal host cell
EP12801652.4A EP2748322A2 (fr) 2011-08-24 2012-08-23 Procédés de production de multiples polypeptides recombinants dans une cellule hôte fongique filamenteuse
CN201280052277.8A CN103890180A (zh) 2011-08-24 2012-08-23 在丝状真菌宿主细胞中产生多种重组多肽的方法
US14/238,691 US9493790B2 (en) 2011-08-24 2012-08-23 Methods for producing multiple recombinant polypeptides in a filamentous fungal host cell
BR112014004190A BR112014004190A2 (pt) 2011-08-24 2012-08-23 método para construir uma cepa fúngida filamentosa, cepa fúngida filamentosa, método para produzir múltiplos polipeptídeos recombinantes, e, construto em tandem
IN2136CHN2014 IN2014CN02136A (fr) 2011-08-24 2012-08-23
US15/280,706 US10301610B2 (en) 2011-08-24 2016-09-29 Filamentous fungal host cell for producing multiple recombinant polypeptides
US16/394,951 US10822601B2 (en) 2011-08-24 2019-04-25 Methods for producing multiple recombinant polypeptides in a filamentous fungal host cell

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201161526809P 2011-08-24 2011-08-24
US61/526,809 2011-08-24

Related Child Applications (2)

Application Number Title Priority Date Filing Date
US14/238,691 A-371-Of-International US9493790B2 (en) 2011-08-24 2012-08-23 Methods for producing multiple recombinant polypeptides in a filamentous fungal host cell
US15/280,706 Division US10301610B2 (en) 2011-08-24 2016-09-29 Filamentous fungal host cell for producing multiple recombinant polypeptides

Publications (2)

Publication Number Publication Date
WO2013028912A2 WO2013028912A2 (fr) 2013-02-28
WO2013028912A3 true WO2013028912A3 (fr) 2013-06-06

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2012/052143 WO2013028912A2 (fr) 2011-08-24 2012-08-23 Procédés de production de multiples polypeptides recombinants dans une cellule hôte fongique filamenteuse

Country Status (7)

Country Link
US (3) US9493790B2 (fr)
EP (1) EP2748322A2 (fr)
CN (2) CN109136114A (fr)
AU (1) AU2012298799B2 (fr)
BR (1) BR112014004190A2 (fr)
IN (1) IN2014CN02136A (fr)
WO (1) WO2013028912A2 (fr)

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WO2013028912A2 (fr) 2011-08-24 2013-02-28 Novozymes, Inc. Procédés de production de multiples polypeptides recombinants dans une cellule hôte fongique filamenteuse
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WO2013174927A1 (fr) 2012-05-23 2013-11-28 Novartis International Pharmaceutical Limited Production de glycoprotéines fucosylées
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ES2896153T3 (es) 2014-09-23 2022-02-24 Novozymes As Procedimientos para producir etanol y organismos de fermentación
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EP3423577B1 (fr) 2016-03-02 2024-05-08 Novozymes A/S Variants de cellobiohydrolase et polynucléotides codant pour ces derniers
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CN115175923A (zh) 2019-12-10 2022-10-11 诺维信公司 用于改善的戊糖发酵的微生物
MX2023002490A (es) 2020-09-04 2023-03-09 Novozymes As Organismo fermentador mejorado para la produccion de etanol.
WO2022261003A1 (fr) 2021-06-07 2022-12-15 Novozymes A/S Micro-organisme génétiquement modifié pour une fermentation d'éthanol améliorée
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