WO2011028135A1 - Inhibiteurs de kinases, formes promédicaments de ceux-ci et leur utilisation en thérapie - Google Patents

Inhibiteurs de kinases, formes promédicaments de ceux-ci et leur utilisation en thérapie Download PDF

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Publication number
WO2011028135A1
WO2011028135A1 PCT/NZ2010/000174 NZ2010000174W WO2011028135A1 WO 2011028135 A1 WO2011028135 A1 WO 2011028135A1 NZ 2010000174 W NZ2010000174 W NZ 2010000174W WO 2011028135 A1 WO2011028135 A1 WO 2011028135A1
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WIPO (PCT)
Prior art keywords
bromide
methyl
pyrido
bromo
imidazol
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PCT/NZ2010/000174
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English (en)
Inventor
Jeffrey Bruce Smaill
Adam Vorn Patterson
Guo-Liang Lu
Ho Huat Lee
Amir Ashoorzadeh
Robert Forbes Anderson
William Robert Wilson
William Alexander Denny
Huai-Ling Hsu (Annie)
Andrej Maroz
Stephen Michael Frazer Jamieson
Alexandra Marie Mowday
Kendall Marie Carlin
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Auckland Uniservices Limited
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Priority to CA2771016A priority Critical patent/CA2771016A1/fr
Priority to JP2012527843A priority patent/JP5925680B2/ja
Priority to BR112012004720-5A priority patent/BR112012004720B1/pt
Priority to AU2010290199A priority patent/AU2010290199B2/en
Priority to US13/390,398 priority patent/US9101632B2/en
Priority to MX2012002327A priority patent/MX336332B/es
Priority to RU2012112396/04A priority patent/RU2568639C2/ru
Priority to CN201080039079.9A priority patent/CN102574846B/zh
Application filed by Auckland Uniservices Limited filed Critical Auckland Uniservices Limited
Priority to EP10814010.4A priority patent/EP2473509A4/fr
Priority to NZ598144A priority patent/NZ598144A/en
Publication of WO2011028135A1 publication Critical patent/WO2011028135A1/fr
Priority to ZA2012/00994A priority patent/ZA201200994B/en
Priority to IL218182A priority patent/IL218182B/en
Priority to US14/788,507 priority patent/US20160031877A1/en

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D471/00—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, at least one ring being a six-membered ring with one nitrogen atom, not provided for by groups C07D451/00 - C07D463/00
    • C07D471/02—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, at least one ring being a six-membered ring with one nitrogen atom, not provided for by groups C07D451/00 - C07D463/00 in which the condensed system contains two hetero rings
    • C07D471/04—Ortho-condensed systems
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/495—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with two or more nitrogen atoms as the only ring heteroatoms, e.g. piperazine or tetrazines
    • A61K31/505—Pyrimidines; Hydrogenated pyrimidines, e.g. trimethoprim
    • A61K31/519—Pyrimidines; Hydrogenated pyrimidines, e.g. trimethoprim ortho- or peri-condensed with heterocyclic rings
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P19/00—Drugs for skeletal disorders
    • A61P19/08—Drugs for skeletal disorders for bone diseases, e.g. rachitism, Paget's disease
    • A61P19/10—Drugs for skeletal disorders for bone diseases, e.g. rachitism, Paget's disease for osteoporosis
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D403/00—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, not provided for by group C07D401/00
    • C07D403/02—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, not provided for by group C07D401/00 containing two hetero rings
    • C07D403/12—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, not provided for by group C07D401/00 containing two hetero rings linked by a chain containing hetero atoms as chain links

Definitions

  • the present invention relates to kinase inhibitors, to kinase inhibitors in prodrug
  • compositions and medicaments containing them, and processes for the preparation and such inhibitors, compositions and medicaments.
  • kinases represent a large family of enzymes that catalyse the phosphorylation of proteins, lipids and metabolites and play a central role in d e regulation of a wide variety of cellular processes. Abnormal kinase activity has been related to a wide range of disorders, including cancers. This has led to the development of kinase inhibitors as therapeutic agents.
  • This invention generally relates to compounds having activity as kinase inhibitors, including their prodrug forms, as well as to the application of such compounds in therapy.
  • the invention provides a kinase inhibitor of Formula I:
  • R t is H
  • R 2 is (3-chlorobenzyl)oxy- and R 3 is chloro;
  • R 2 and R 3 together with the carbon atoms to which they are attached, form l-(3- fiuorobenzyl)-l.H-pyrazole;
  • R 2 is 2-pyticlmylmethoxy and R 3 is chloro;
  • R 2 and R 3 are both chloro
  • R 2 is chloro and R 3 is bromo
  • R 2 and R 3 are both bromo
  • R 2 is fluoro and R 3 is ethynyl
  • R 2 is chloro and R 3 is ethynyl
  • R2 is bromo and R 3 is ethynyl
  • R 2 is bromo and R 3 is fluoro
  • R 2 is 2-pyridinylmethoxy and R 3 is fluoro;
  • R 2 is 2-pyridinylmethoxy and R 3 is bromo;
  • R negligence R 2 and R is selected from benzyloxy, 3-chlorobenzyloxy and 2- pyridinylmethoxy and when at least one of R l5 R 2 and R 3 is not benzyloxy, 3- chlorobenzyloxy or 2-pyridinylmethoxy, each of the others is independendy selected from H, halogen, and C 2 -C 4 alkynyl, with the proviso that when one of R,, R 2 and R 3 is benzyloxy or 2-pyridinylmethoxy, the other two of R 1? R, and R 3 are not H; or
  • the invention provides kinase inhibitors of the Formula IA
  • R a is H, and either (a) R 2 is (3-chlorobenzyl)oxy- and R 3 is chloro;
  • R 2 is 2-pyridinykaethoxy and R 3 is chloro;
  • R 2 and R are botli chloro
  • R 2 is chloro and R 3 is bromo
  • R 2 and R 3 are both bromo
  • R 2 is fluoro and R 3 is ethynyl
  • R 2 is chloro and R 3 is ethynyl
  • R 2 is bromo and R 3 is ethynyl
  • R 2 is bromo and R 3 is fluoro
  • R 2 is 2-pyridinylmethoxy and R 3 is fluoro;
  • R 2 is 2-pyridinylmethoxy and R 3 is bromo;
  • the kinase inhibitor is selected from the following:
  • the invention provides the use of a kinase inhibitor of Formula I as defined above, or a salt or solvate thereof, in the preparation of a medicament.
  • the medicament is a prodrug and the compound of Formula I or the salt or solvate diereof is linked to a reductive trigger.
  • the invention provides a reductive prodrug comprising a kinase inhibitor of Formula I as defined above, or a salt or solvate thereof, and a reductive trigger linked directly or indirectly to a nitrogen of the kinase inhibitor.
  • the reductive trigger is of Formula II:
  • R 5 is selected from C C 6 alkyl and R 4 is selected from H, methyl, ethyl, trifiuoromethyl, -CN, -CONH 2 and propyn-1-yl.
  • the reductive trigger is selected from the group consisting of Formulae Ila to Iig:
  • R 5 is selected from methyl, ethyl and propyl, preferably methyl.
  • the reductive trigger is of Formula Ila, wherein R 5 is selected from methyl, ethyl and propyl.
  • the reductive trigger is of Formula Ila, wherein R 5 is selected from methyl.
  • the prodrug is a compound of Formula III:
  • R 1? R 2 and R 3 are as defined for Formula I, R 4 is selected from H, methyl, ethyl, trifluoromethyl, -CN, -CONH, and propyn-1-yl, and R 5 is Q-C 6 alkyl.
  • R t , R 2 and R 3 are as defined for Formula I and R 4 is selected from H, methyl, ethyl, trifluoromethyl, -CN, -CONH 2 and propyn-1-yl.
  • R,, R 2 and R 3 are as defined for Formula IA and R 4 is selected from H, methyl, ethyl, trifluoromethyl, -CN, -CONH 2 and propyn-1-yl.
  • X is selected from halide (fluoride, chloride, bromide, iodide), methanesulfonate, tiifluoromethanesulfonate, acetate, trifluoroacetate, tosylate, lactate, citrate and formate.
  • X is halide, with bromide being most preferred.
  • the compound of Formula III is selected from:
  • the invention provides a compound of Formula I or salt or solvate thereof as defined above, a reductive prodrug as defined above, or a compound of Formula III or salt or solvate diereof as defined above, for use in therapy.
  • the invention provides pharmaceutical compositions comprising a compound of Formula I or salt or solvate thereof as defined above, a reductive prodrug as defined above, or a compound of Formula III or salt or solvate thereof as defined above, in combination with one or more pharmaceutically acceptable excipients or diluents.
  • the invention provides a method of therapy which includes the step of administering a therapeutically effective amount of a compound of Formula I or salt or solvate thereof as defined above, a reductive prodrug as defined above, or a compound of Formula III or salt or solvate thereof as defined above, to a patient in need of such therapy.
  • the method of therapy is the treatment of cancer.
  • die invention provides a method of inliibiting kinase activity within a subject which involves the step of administering to said subject an inhibitory amount of a compound of Formula I or salt or solvate thereof as defined above, a reductive prodrug as defined above, or a compound of Formula III or salt or solvate thereof as defined above.
  • said inhibiting of kinase activity is for a therapeutic purpose, including an anti-cancer purpose.
  • the present invention provides a method for the production of an anti-cancer effect in a warm-blooded animal such as a human, wherein the method comprises administering to the animal an effective amount of a compound of Formula I or salt or solvate thereof as defined above, a reductive prodrug as defined above, or a compound of Formula III or salt or solvate thereof as defined above.
  • the present invention provides a method for the production of an anti-cancer effect in a cell, wherein the method comprises contacting the cell with an effective amount of a compound of Formula I or salt or solvate thereof as defined above, a reductive prodrug as defined above, or a compound of Formula III or salt or solvate thereof as defined above.
  • the present invention provides a method for the treatment of a cancer in a warm-blooded animal such as a human, which comprises administering to the animal an effective amount of a compound of Formula I or salt or solvate thereof as defined above, a reductive prodrug as defined above, or a compound of Formula III or salt or solvate thereof as defined above.
  • Figure 12 shows inhibition of erbBl (EGFR) autophosphorylation and p44/42 MAPK(Erk 1/2) phosphorylation in intact A431 cells following lhour treatment with a range of concentrations of compound 3;
  • Figure 13 shows inhibition of erbBl (EGFR) autophosphorylation and p44/42 MAPK(Erk 1/2) phosphorylation in intact A431 cells following lhour treatment with a range of concentrations of compound 5;
  • Figure 14 shows inhibition of erbBl (EGFR) autophosphorylation and p44/42 MAPK(Erk 1/2) phosphorylation in intact A431 cells following lhour treatment with a range of concentrations of compound 6;
  • Figure 15 shows inhibition of erbBl (EGFR) autophosphorylation and p44/42 MAPK(Erk 1/2) phosphorylation in intact A431 cells following lhour treatment with a range of concentrations of prodrug 15.
  • Figure 16 is a graphical plot of band desitometry analysis of relative ( ⁇ -actin corrected) inhibition of erbBl (EGFR) autophosphorylation in intact A431 cells following lhour treatment with a range of concentrations of prodrug 15 and compound 3.
  • alkyl and alkynyl include both straight chain and branched chain groups, and unsubstituted and substituted groups.
  • the optional substituents may include, without limitation, halogen, Q-Q alkoxy, CN, OH, NH 2 , N0 2 , NH(C r C 6 alkyl), N(C r C 6 alkyl) 2 , CONH 2 , CO(C r C, alkyl), SO,NH 2 and S0 2 (C r C 6 alkyl).
  • Anti-cancer effects include, but are not limited to, anti-tumour effects, the response rate, the time to disease progression and the survival rate.
  • Anti-tumour effects include but are not limited to inhibition of tumour growth, tumour growth delay, regression of tumour, shrinkage of tumour, increased time to regrowth of tumour on cessation of treatment and slowing of disease progression.
  • Effective amount means an amount of a compound that, when administered to a subject for treating a cancer, is sufficient to effect such treatment for the cancer.
  • the “effective amount” will vary depending on the cancer to be treated, the compound to be administered, the severity of the cancer treated, the age and relative health of the subject, the route and form of administration, whether the treatment is monotherapy or combination therapy, the judgement of the attending clinician, and other factors.
  • “Pharmaceutically acceptable” means that which is useful in preparing a pharmaceutical composition that is generally safe, non-toxic, and neither biologically nor otherwise undesirable and includes that which is acceptable fo veterinary as well as human pharmaceutical use.
  • “Pharmaceutically acceptable salts” of a compound means salts that are pharmaceutically acceptable, as defined herein, and that possess the desired pharmacological activity of the parent compound. Such salts include:
  • acid addition salts formed with inorganic acids such as hydrochloric acid, hydrobromic acid, sulfuric acid, nitric acid, phosphoric acid and the like; or formed with organic acids such as acetic acid, methanesulfonic acid, maleic acid, tartaric acid, citric acid and the like; or
  • a metal ion e.g. an alkali metal ion, an alkaline earth ion, or an aluminium ion
  • Acceptable organic bases include ethanolamine, diethanolamine, N- metiiylglucamine, triethanolamine and the like.
  • Acceptable inorganic bases include aluminium hydroxide, calcium hydroxide, potassium hydroxide, sodium carbonate and sodium hydroxide.
  • Warm blooded animal means any member of the mammalia class including, but not limited to humans, non-human primates such as chimpanzees and other apes and monkey species, farm animals such as cattle, horses, sheep, goats, and swine; domestic animals such as rabbits, dogs and cats; laboratory animals including rodents, such as rats, mice and guinea pigs, and the like.
  • farm animals such as cattle, horses, sheep, goats, and swine
  • domestic animals such as rabbits, dogs and cats
  • laboratory animals including rodents, such as rats, mice and guinea pigs, and the like.
  • the invention relates to compounds useful as kinase inhibitors. Such compounds have application in therapy.
  • the compounds of the invention are 4-anil opyrido[3,4- ⁇ pyriniidine" irreversible erbBl, 2, 4 kinase inhibitors of Formula I.
  • the compounds comprise a kinase inhibitor of Formula I and a reductive trigger.
  • the reductive trigger fragments when reduced.
  • fragmentation of the trigger occurs at the one-electron reduction level and is effectively suppressed by the presence of oxygen, thus providing selective activation in hypoxic environments.
  • This suppression by oxygen may occur through reoxidation of the one-electron radical by oxygen, or by oxidation of reducing intermediates required for prodrug reduction.
  • the latter would include, for example, scavenging by oxygen of radiation-induced reducing radicals such as the aquated electron, or oxidation of reducing intermediates in the catalytic cycle of reductase enzymes.
  • the reduction equivalents required to reduce the trigger may be provided by enzymes, radiation-induced radicals, or chemical reducing agents.
  • the trigger is a reductively-activated aromatic nitroheterocycle or aromatic nitrocarbocycle trigger and is linked directly to a quaternisable nitrogen of a compound of Formula I such that a quaternary nitrogen is formed. It is however particularly preferred that the trigger has the structure of Formula II above.
  • the compounds act as prodrugs, with reduction of the trigger releasing the kinase inhibitor. It is presently most preferred that the prodrug forms of the compounds of the invention are of Formula III as defined above.
  • the compounds of the invention have application in any therapeutic approach in which inhibition of the activity of a kinase is desirable.
  • the invention therefore relates to methods for treating and preventing diseases, for example, hyper-proliferative, inflammatory and angiogenesis disorders and osteoporosis in mammals by adrninistering a compound of this invention or a pharmaceutical- composition comprising one or more compounds of this invention.
  • the invention particularly relates to a metiiod of treating or preventing cancer and other
  • hyperproliferative disorders by administering a compound of this invention or a pharmaceutical composition comprising one or more compounds of tiiis invention, whether alone as a
  • Optional anti-proliferative agents which can also be adrninstered include but are not limited to compounds listed on the cancer chemotherapy drug regimens in the 14 th Edition of the Merck Index (2006), which is hereby incorporated by reference, such as asparaginase, bleomycin, carboplatin, carmustine, chlorambucil, cisplatin, colaspase, cyclophosphamide, cytarabine, dacarbazine, dactinomycin, daunorubicin, doxorubicin (adriamycine), epirubicin, etoposide, 5-fluorouracil, hexamediylmelamine, hydroxyurea, ifosfamide, irinotecan, leucovorin, lomustine, mecHorethamine, 6-mercaptopurine, mesna, methotrexate, mitomycin C, mitoxantrone, prednisolone, predn
  • Additional anti-proliferative agents include other molecular targeted agents which modulate parallel pathways such as MEK 1/2 inhibitors, AKT inhibitors and mTOR inhibitors, monoclonal antibodies, oxaliplatin, gemcitabine, gefinitib, taxotere, ara A, ara C, herceptin, BCNU, CCNU, DTIC, and actinomycin D. Still further anti-proliferative agents include but are. not limited to those compounds acknowledged to be used in the treatment of neoplastic diseases in Goodman and Gil an's The Pharmacological Basis of Therapeutics (Eleventh Edition), editor Molinoff et al., publ.
  • Cancer and hyperproliferative disorders as used herein include but are not limited to solid tumors, such as cancers of the breast, respiratory tract, brain, reproductive organs, digestive tract, urinary tract, eye, liver, sldn, head and neck, thyroid, parathyroid and their distant metastases. Those disorders also include lymphomas, sarcomas, and leukemias.
  • breast cancer examples include, but are not limited to invasive ductal carcinoma, invasive lobular carcinoma, ductal carcinoma in situ, and lobular carcinoma in situ.
  • cancers of the respiratory tract include, but are not limited to small-cell and non-small- cell lung carcinoma, as well as bronchial adenoma and pleuropulmonary blastoma.
  • brain cancers include, but are not limited to brain stem and hypophthalmic glioma, cerebellar and cerebral astrocytoma, medulloblastoma, ependymoma, as well as neuroectodermal and pineal tumor.
  • Tumors of the male reproductive organs include, but are not limited to prostate and testicular cancer.
  • Tumors of the female reproductive organs include, but are not limited to endometrial, cervical, ovarian, vaginal, and vulvar cancer, as well as sarcoma of the uterus.
  • Tumors of the digestive tract include, but are not limited to anal, colon, colorectal, esophageal, gallblader, gastric, pancreatic, rectal, small-intestine, and salivary gland cancers.
  • Tumors of the urinary tract include, but are not limited to bladder, penile, kidney, renal pelvis, ureter, and urethral cancers.
  • Eye cancers include, but are not limited to intraocular melanoma and retinoblastoma.
  • liver cancers include, but are not limited to hepatocellular carcinoma (liver cell carcinomas with or without fibrolamellar variant), cholangiocarcinoma (intrahepatic bile duct carcinoma), and mixed hepatocellular cholangiocarcinoma.
  • Skin cancers include, but are not limited to squamous cell carcinoma, Kaposi's sarcoma, malignant melanoma, Merkel cell skin cancer, and non-melanoma skin cancer.
  • Head-and-neck cancers include, but are not limited to
  • Lymphomas include, but are not limited to AIDS-related lymphoma, non-Hodgkin's lymphoma, cutaneous T-cell lymphoma, Hodgkin's disease, and lymphoma of the central nervous system.
  • Sarcomas include, but are not limited to sarcoma of the soft tissue, osteosarcoma, malignant fibrous histiocytoma, lymphosarcoma, and rhabdomyosarcoma.
  • Leukemias include, but are not limited to acute myeloid leukemia, acute lymphoblastic leukemia, chronic lymphocytic leukemia, chronic myelogenous leukemia, and hairy cell leukemia.
  • Conditions within a human or other warm-blooded animal which can be treated by aciministering a compound of this invention include tumor growth, retinopathy, including diabetic retinopathy, ischemic retinal-vein occlusion, retinopathy of prematurity and age related macular degeneration; psoriasis, or bullous disorder associated with subepidermal blister formation, including bullous pemphigoid, erythema multiforme, or dermatitis herpetiformis, rheumatoid arthritis, osteoarthritis, septic arthritis, tumor metastasis, periodontal disease, cornal ulceration, proteinuria, coronary thrombosis from atherosclerotic plaque, aneurismal aortic, dystrophobic epidermolysis bullosa, degenerative cartilage loss following traumatic joint injury, osteopenias mediated by MMP activity, tempero mandibular joint disease or demyelating disease of the nervous system.
  • Therapeutic dosages will likely be in the range of 1 mg to 3000 mg per day.
  • the specific dose level selected for any particular patient will depend upon a variety of factors, including the activity of the specific compound employed, the age, body weight, general health, sex, diet, time of administration, route of adniinistration, and rate of excretion, drug combination and the severity of the condition undergoing therapy.
  • the invention also includes pharmaceutical compositions including a compound of this invention, and a pharmaceutically acceptable carrier.
  • the compounds may be a ⁇ dministered orally, topically, parentetally, by inhalation or spray or rectally in dosage unit formulations.
  • the term 'administration by injection' includes intravenous,
  • One or more compounds may be present in association with one or more non-toxic pharmaceutically acceptable carriers and if desired other active ingredients.
  • compositions intended for oral use may be prepared according to any suitable method known to the art for the manufacture of pharmaceutical compositions.
  • Such compositions may contain one or more agents selected from the group consisting of diluents, sweetening agents, flavoring agents, coloring agents and preserving agents in order to provide palatable preparations.
  • Tablets contain the active ingredient in admixture with non-toxic pharmaceutically acceptable excipients which are suitable for the manufacture of tablets.
  • excipients may be, for example, inert diluents, such as calcium carbonate, sodium carbonate, lactose, calcium phosphate or sodium phosphate; granulating and disintegrating agents, for example, corn starch, or alginic acid; and binding agents, for example magnesium stearate, stearic acid or talc.
  • the tablets may be uncoated or they may be coated by known techniques to delay disintegration and adsorption in the gastrointestinal tract and thereby provide a sustained action over a longer period.
  • a time delay material such as glyceryl monostearate or glyceryl distearate may be employed.
  • These compounds may also be prepared in solid, rapidly released form.
  • Formulations for oral use may also be presented as hard gelatin capsules wherein the active ingredient is mixed with an inert solid diluent, for example, calcium carbonate, calcium phosphate or kaolin, or as soft gelatin capsules wherein the active ingredient is mixed with water or an oil medium, for example peanut oil, liquid paraffin or olive oil.
  • an inert solid diluent for example, calcium carbonate, calcium phosphate or kaolin
  • water or an oil medium for example peanut oil, liquid paraffin or olive oil.
  • Aqueous suspensions contain the active materials in admixture with excipients suitable for the manufacture of aqueous suspensions.
  • excipients are suspending agents, for example sodium carboxymethylcellulose, methylcellulose, hydroxypropyl methylcellulose, sodium alginate, polyvinylpyrrolidone, gum tragacanth and gum acacia; dispersing or wetting agents may be a naturally occurring phosphatide, for example, lecithin, or condensation products or an alkylene oxide with fatty acids, for example polyoxyethylene stearate, or condensation products of ethylene oxide with long chain aliphatic alcohols, for example heptadecaethylene oxycetanol, or condensation products of ethylene oxide with partial esters derived from fatty acids and hexitol such as polyoxyethylene sorbitol monooleate, or condensation products of ethylene oxide with partial esters derived from fatty acids and hexitol anhydrides, for example polyethylene sorbitan monooleate.
  • the aqueous suspensions may also contain one or more preservatives, for example ethyl, or n-propyl p- hydroxybenzoate, one or more coloring agents, one or more flavoring agents, and one or more sweetening agents, such as sucrose or saccharin.
  • preservatives for example ethyl, or n-propyl p- hydroxybenzoate
  • coloring agents for example ethyl, or n-propyl p- hydroxybenzoate
  • flavoring agents for example ethyl, or n-propyl p- hydroxybenzoate
  • sweetening agents such as sucrose or saccharin.
  • Dispersible powders and granules suitable for preparation of an aqueous suspension by the addition of water provide the active ingredient in admixture with a dispersing or wetting agent, suspending agent and one or more preservatives.
  • a dispersing or wetting agent e.g., talc, kaolin, kaolin, kaolin, kaolin, kaolin, kaolin, kaolin, kaolin, kaolin, kaolin, kaolin, kaolin, kaolin, kaolin, kaolin, sorbitol, sorbitol, sorbitol, sorbitol, sorbitol, sorbitol, sorbitol, sorbitol, sorbitol, sorbitol, sorbitol, mannitol, mannitol, mannitol, mannitol, mannitol, mannitol, mannitol, mannitol,
  • the compounds may also be in the form of non-aqueous liquid formulations, e.g., oily suspensions which may be formulated by suspending the active ingredients in a vegetable oil, for example arachis oil, olive oil, sesame oil or peanut oil, or in a mineral oil such as liquid paraffin.
  • the oily suspensions may contain a tliickening agent, for example beeswax, hard paraffin or cetyl alcohol. Sweetening agents such as those set forth above, and flavoring agents may be added to provide palatable oral preparations. These compositions may be preserved by the addition of an anti-oxidant such as ascorbic acid.
  • compositions of the invention may also be in the form of oil-in-water emulsions.
  • the oily phase may be a vegetable oil, for example olive oil or arachis oil, or a mineral oil, for example liquid paraffin or mixtures of these.
  • Suitable emulsifying agents may be naturally-occurring gums, for example gum acacia or gum tragacanth, naturally-occurring phosphatides, for example soy bean, lecimin, and esters or partial esters derived from fatty acids and hexitol anhydrides, for example sorbitan monooleate, and condensation products of the said partial esters with ethylene oxide, for example polyoxyethylene sorbitan monooleate.
  • the emulsions may also contain sweetening and flavoring agents.
  • Syrups and elixirs may be formulated with sweetening agents, for example glycerol, propylene glycol, sorbitol or sucrose. Such formulations may also contain a demulcent, a preservative and flavoring and coloring agents.
  • sweetening agents for example glycerol, propylene glycol, sorbitol or sucrose.
  • Such formulations may also contain a demulcent, a preservative and flavoring and coloring agents.
  • compositions can be prepared by mixing the drug with a suitable non-irritating excipient which is solid at ordinary temperatures but liquid at the rectal temperature and will therefore melt in the rectum to release the drug.
  • suitable non-irritating excipient include cocoa butter and
  • the prodrug compounds of the present invention comprise an effector moiety linked to a reductive trigger, which is preferably a nitroheterocyclic reductive trigger of Formula II, more preferably of Formula Ila, as defined above.
  • the effector moiety is a 4-anilinopyrido[3,4- ⁇ pyrirrddine irreversible erbBl, 2, 4 kinase inhibitor of Formula I.
  • the 4-amlinopyrido[3,4- ⁇ pyrimidine irreversible erbBl , 2, 4 kinase inhibitors of Formula I possess an amide Michael acceptor in the 6-position, where the Michael acceptor features a double bond that is substituted at the beta carbon with a methylene group that terminates witii a tertiary climethylamine group, as shown below.
  • the preferred prodrug compounds are of Formula III as defined above.
  • the preferred prodrug compounds of Formula III may be prepared by quaternising an aliphatic tertiary climethylamine effector moiety with a nitroheterocyclic reductive trigger moiety. Such methods are described in more detail below.
  • Effector compounds related to those of the Formula I as shown above, where the central inhibitor scaffold is a 4-anilinopyrido[3,4- ⁇ pyrimidine and the 6-position is substituted with an amide Michael acceptor may be prepared according to methods described in the art when the amide Michael acceptor, for example, is unsubstituted (Smaill et al. J Med Chem, 1999, 42, 1803-1815), substituted at the iV-position, alpha-position or beta-position with a range of groups (Smaill et al.
  • Scheme 1 illustrates the preparation of 4-anirinopyrido[3,4- ⁇ pyrirmdine effector compounds of the invention, from the loiown intermediate 6-fluoropytido[3,4- ⁇ pyrimidinone (200) (Rewcasde et al, J Chem Soc, Perkins Trans 1, 1996, 2221-2226) using the methods of Rewcasde et al, J Chem Soc, Perkins Trans 1, 1996, 2221-2226 and Soyka et al, US 2005/0085495 Al.
  • the prodrug compounds of the invention may, in general terms, be prepared by reacting an aliphatic tertiary amine-bearing kinase inhibitor of Formula I with an appropriate nitroheterocyclic or nitrocarbocylic -methyl halide/mesylate/ tosylate, in a suitable solvent and for a suitable length of time (for example in JV-methyl-2-pyrrolidinone for about 15 hours), to produce a quaternary nitrogen salt comprising the nitroheterocyclic or nitrocarbocyclic reductive trigger moiety linked directly or indirectly to a nitrogen of the kinase inhibitor.
  • Preferred reductive trigger moieties suitable for use in the prodrugs of the invention are of Formula II shown below:
  • a-methyl halides of Formula lla may be prepared as described previously (bromide; Stribbling et al, PCT International patent publication WO 2008/039087) (chloride; Tercel et al, J Med Chem, 2001, 44, 3511-3522).
  • Scheme 5 illustrates a route to the a-methyl bromide 250, from 1,5-dimethyl-4-nitro-1H- imidazole (238) (Scheme 3).
  • Scheme 7 illustrates two alternate routes to die a-methyl bromide 264, from a-methyl bromide 246 (Scheme 5) and 2-bromo-l J 5-dimemyl-4- tro-1H-imidazole (245) (Scheme 5), respectively.
  • Scheme 7 below also illustrates a route to the a-methyl bromide 266, from a-methyl bromide 264.
  • Scheme 8 illustrates a route to the a-methyl bromide 270, from (2-bromo-1-methyl-4-nitro ⁇ 1 H-imidazol-5-yl)methyl acetate (247) (Scheme 5).
  • Scheme 9 illustrates a route to quaternary nitrogen salt compounds of Formula III by reacting an aliphatic tertiary amine-bearing kinase inhibitor of Formula I with an appropriate
  • nitroheterocyclic a-methyl halide/mesylate/tosykte (Formula VIII) in a suitable solvent and for a suitable length of time (for example in iV-me yl-2-pyrroHdinone for about 15 hours).
  • Scheme 10 illustrates the preparation of a number of prodrug compounds of Formula III according to the invention.
  • the 4-anilinopyrido[3,4- ⁇ pyrimidine effector compounds 1-9 (Scheme 2) were reacted with the oc-mediyl bromide 239 (Scheme 3) in iV-methyl-2-pyrrolidinone (NMP) at room temperature for approximately 15 hours, before the addition of acetonitrile, to provide the quaternary ammonium salts (12, 13, 15-21) as a fine precipitate that was collected by filtration and washed with acetonitrile, ethyl acetate and hexane.
  • NMP iV-methyl-2-pyrrolidinone
  • Petroleum ether 400 mL was added and stirred at room temperature for 15 min. Petroleum ether layer was decanted. More petroleum ether (300 mL) was added and stirred for 15 min. The solid was collected by suction filtration; washed with water (4 x 30 mL); dried under reduced pressure over silica gel/KOH to give compound 3 (1.79 g, 83%) which was found only 87% pure by HPLC. Thus, the sample was stirred with warm MeOH (200 mL) for 30 min and cooled to room temperature. The insoluble materials were filtered off and to the filtrates was added one volume of water to precipitate out the required product.
  • the reaction mixture was stirred at room temperature for 20 h. It was poured into water (200 mL). The pH was adjusted to ca. 8 using an aqueous solution of Na 2 C0 3 at room temperature. Petroleum ether (300 mL) was added and stirred at room temperature for 30 min. The petroleum ether layer was decanted. It was repeated once more with petroleum ether. The solid was collected by filtration and washed with water (5 x 25 mL).
  • the reaction mixture was stirred at room temperature for 1 h. It was poured into water (200 mL). The pH was adjusted to ca. 8 using an aqueous solution of Na 2 C0 3 at room temperature. Petroleum ether (300 mL) was added and stkred at room temperature for 30 min. The petroleum ether layer was decanted. It was repeated once more with petroleum ether. The solid was collected by filtration and washed with water (4 x 20 mL).
  • the yellow/ orange solid was collected by filtration and washed with water (5 x 20 mL), then with petroleum ether (3 x 30 mL). The sticky yellow/orange solid was dissolved in acetone (150 mL) at 45 °C (bath temperature). It was filtered to remove insoluble impurities. To the filtrate was added water (400 mL) and stkred at room temperature for 1 h.
  • the reaction mixture was stirred at room temperature for 18 h. It was poured into water (250 mL). The pH was adjusted to ca. 8 using an aqueous solution of Na 2 C0 3 at room temperature. Petroleum ether (300 mL) was added and stirred at room temperature for 1 h. The petroleum ether layer was decanted. It was repeated once more with petroleum ether. The solid was collected by filtration and washed with water (4 x 20 mL).
  • the reaction mixture was stirred at room temperature for 19 h. It was poured into water (300 mL). The pH was adjusted to ca. 8 using an aqueous solution of Na 2 C0 3 at room temperature. Petroleum ether (300 mL) was added and stirred at room temperature for 30 min. The petroleum ether layer was decanted. It was repeated once more with petroleum ether. The solid was collected by filtration and washed successively with water (5 x 25 mL) and petroleum ether/ ethyl acetate (10:1) (4 x 25 mL).
  • Anal. Calcd for C 13 H 7 Br 2 FN 4 C, 39.23; H, 1.77; N, 14.08. Found C, 39.23; H, 1.79; N, 13.86.
  • a 20-L reactor was fitted with N 2 , condenser, temperature probe, and air stirring.
  • the reactor was charged with compound 238 (100 g, 0.71 mol), 1,3-dibromo-5,5-dimethylhydantoin (DBDMH) (203.0 g, 0.71 mol), 2,2'-azobisisobutyronitrile (AIBN) (45.97 g, 0.28 mol), and dichloromethane (6 L).
  • the reaction was heated at reflux, stirring the homogenous solution at 150 rpm.
  • Method A Preparation of quaternary ammonium salt prodrugs in N-metbjl-2-pyrtvlidinom (NMP) followed by acetonitrile precipitation.
  • Pan-erbB kinase inhibitors Kinase inhibitors of Formula I (compounds 1-9) were tested for their ability to inhibit the proliferation of three human carcinoma cell lines, selected to provide a comparison with literature precedent: A431 (epidermoid), which overexpresses erbBl (EGFR); H1975 (non-small-cell lung), which overexpresses erbBl L858R/T790M a double mutant form of erbBl that is known to confer resistance to the approved reversible erbBl inliibitor erlotinib and SKOV3 (ovarian), which over express erbB2 (HER2).
  • A431 epidermoid
  • H1975 non-small-cell lung
  • erbBl L858R/T790M a double mutant form of erbBl that is known to confer resistance to the approved reversible erbBl inliibitor erlotinib and SKOV
  • the cells were exposed to test compounds for either 24 hours under oxic conditions or for 4 hours under anoxia followed by 20 hours under oxic conditions. They were then washed free of drug and incubated for a further 4 days, before being stained for cellular growth with sulforhodamine B.
  • Selected prodrug compounds of Formula III were tested for their ability to inhibit the proliferation of three human carcinoma cell lines, selected to provide a comparison with literature precedent: A431 (epidermoid), which overexpresses erbBl (EGFR); H1975 (non-small-cell lung), which overexpresses erbBl L858R/T790M a double mutant form of erbBl that is known to confer resistance to the approved reversible erbBl inhibitor erlotinib and S OV3 (ovarian), which over express erbB2 (HER2).
  • the cells were exposed to test compounds for either 24 hours under oxic conditions or for 4 hours under anoxia followed by 20 hours under oxic conditions.
  • IC50 value concentration of compound required to inhibit cellular growth by 50% relative to untreated control wells
  • a compound dose-response curves were determined at 10 concentrations.
  • Cells received a 24 hour exposure to test compounds before being washed (x3) with drug-free media.
  • the IC50 (umol/L) values are the concentrations required to inhibit cell growth by 50% relative to untreated controls. Values are the average of 2-8 independent determinations (%CV ⁇ 20 in all cases).
  • b Experiment performed entirely under oxic conditions.
  • c The initial 4 hours of the 24 hour drug exposure was performed under anoxic conditions.
  • d Hypoxic Cytotoxicity Ratio fold change in intra- experimental IC50 for cells receiving 4 hours of anoxia relative to cells that received only oxic conditions.
  • HCR hypoxic cytotoxicity ratios
  • the compounds 3, 5 and 6 were tested for their ability to inhibit the autophosphorylation of erbBl (EGFR), and p44/42 MAPK (Erkl/2) in EGF-stimulated A431 cells by Western immunoblotting measurement of phospho-erbBl and phospho-Erk /2 status.
  • A43 cells were seeded into 6 well plates (with ctMEM + 5% FCS). The following day, plates were washed once with serum-free medium and grown for 18 hours in serum-free medium (aMEM) before being exposed to a range of inhibitor or prodrug concentrations for 1 hour and then stimulated for 15 minutes with 100 ng/ml epidermal growth factor (EGF receptor ligand).
  • aMEM serum-free medium
  • the monoclonal antibody (1:500; Cell Signalling #2234) was incubated overnight at 4°C and binding was detected with goat-anti-Rabbit-IgG-HRP conjugated secondary antibody (1:5000; Santa Cruz #SC2054; 3h RT).
  • the monoclonal antibody (Cell Signalling #4370) was used at a 1:500 dilution. The blot was then treated with Goat anti-rabbit IgG- HRP conjugated secondary antibody (Santa Cruz Biotechnology,Inc. sc-2054) for three hours at a ratio of 1:5000.
  • each blot was probed with anti-p-actin antibody (Abacus ALS # MAB-1501R, 1:2000 dilution). The following day, the blot was treated with Goat anti-mouse IgG— HRP conjugated secondary antibody (Santa Cruz Biotechnology, Inc. sc-2055) for one hour at a 1:5000 ratio. Protein bands were visualized using Amersham ECL Plus Western Blotting Detection Reagent (GE Healthcare RPN2132). Densitometry was determined using Image J software. Values normalized to ⁇ -actin were plotted on SigmaPlot 11.0..
  • Electton-affinic prodrugs can be selectively reduced by 1 -electron processes in the hypoxic regions of solid tumours, in contrast to under normoxic conditions in normal tissues, to form or release a cytotoxic effector (Brown and Wilson, Nature Rev. Cancer, 2004, 4, 437-447).
  • the prodrug should contain a trigger moiety possessing a 1 -electron reduction potential, E(l), of between -0.6V to -0.2 V and preferably between -0.5 V to -0.3V vs. NHE.
  • E(l) 1 -electron reduction potential
  • the B(l) values of many compounds can be obtained from the literature, (for example, Wardman, P. J. Phys. Chem. Ref.
  • the pulse radiolysis method measures the equiHbrium constant between the radical anions of the prodrugs, formed upon their 1 -electron reduction, and reference standards such as viologen and quinone compounds, from which data the E(t) values of the compounds can be calculated.
  • the J3(l) values of prodrugs 13, 15-19 were measured by the pulse radiolysis method and determined to range between -0.428V and -0.417V (Table 3). All are considered to possess appropriate £(1) values to enable enzymatic formation of their radical anions in a biological context.
  • Prodrugs possessing appropriate 23(1) values can be tested for their ability to release effector moieties by a number of methods, following the radiolysis of the prodrugs in solution. For example, mass spectrometry (MS) and/ or high performance liquid chromatography (HPLC) before and after radiolysis identifies the starting compound and the products formed as a result of the radiolysis.
  • MS mass spectrometry
  • HPLC high performance liquid chromatography
  • prodrug 17 is consistent with its consumption at the 1-electron reduction level (>50% loss of prodrug at the 0.95 reducing equivalents level) with the released effector (compound 5) detected by HPLC-MS, Table 3.
  • the reductive prodrugs are selected to have controlled fragmentation rate constants upon 1-electron reduction of the trigger moiety. Whilst fast fragmentation to release high .
  • concentrations of the cytotoxic effectors in the hypoxic regions of tumour cells is desirable, this is not so for normal tissue cells under normoxia.
  • the rate constant of the back oxidation of the 1-electron reduced nitroarene-based prodrugs by oxygen, k 2 which effectively inhibits the release of the effector, is given by the expression:- (Wardman et al, Biochem. Soc. Symp., 1995, 61, 171-194;
  • B(1)C/C- is the 1-electron reduction potential of the prodrug.
  • E(l) the pseudo ⁇ -order rate constants for this back oxidation in normal cells (which can be, under physiological conditions, as low as 10 ⁇ in oxygen concentration) decrease from 130 s -1 to 13 s -1 .
  • the ranges in fragmentation rate to allow for sufficient back-oxidation in normoxic tissue and therefore to impart hypoxia-selective fragmentation to the prodrug is most preferably in the region of ca.
  • the rate constants for fragmentation, /3 ⁇ 4frag, of the 1-electron reduced prodrugs can be measured using pulse radiolysis to observe the time-resolved formation of the absorption spectrum of tlie benzyl-type radical produced upon fragmentation of the radical anion. (Anderson et al, J. Phys. Chem. A, 1997, 101, 9704-9709.)
  • the 3 ⁇ 4frag values of prodrugs 13, 15-17 were measured by pulse radiolysis and are presented in Table 3.
  • prodrugs possess fragmentation rates upon 1- electron reduction under hypoxia in the desirable range, consistent with them showing acceptable hypoxic cytotoxicity ratios (HCRs) in vitro in A431, HI 975 and S OV3 cell-based anti-proHferative assays (Table 3).
  • HCRs hypoxic cytotoxicity ratios
  • Table 3 Radiolytic reduction of selected prodrugs by the C0 2 ° ⁇ radical.
  • example prodrugs were dissolved in Millipore water (containing added 50 mM sodium formate buffered at pH 7 by 5 mM sodium phosphate) at a concentration of 50 ⁇ or below. Solutions, contained in air-tight glassware continuously saturated with ⁇ , ⁇ gas for 30 mins prior to radiolysis at a dose rate of 7.5 Gy min -1 , previously determined using Fricke dosimetry (Fricke and Hart, "Chemical Dosimetry" in Radiation Dosimetry Vol.
  • Pulse radiolysis was used to monitor the 1-electron reduction and stability of the compounds in real time.
  • a linear accelerator delivering short pulses of high energy electrons (2-3 Gy in 200 ns of 4MeV) equipped with a fast spectophotometric detection system was used.
  • Prodrugs were dissolved in N 2 0-saturated solutions containing formate ions, as above, which, following pulse radiolysis, resulted in the rapid formation of the radical anions of the compounds within a few microseconds.
  • the rate of fragmentation was determined by analysing kinetic transients at wavelengths corresponding to the formation of the benzyl-type radical of the trigger moiety.
  • mice Specific pathogen-free female N1H-III nude mice, derived from breeding mice supplied by Charles River Laboratories (Wilmington, MA), were housed in groups of 4-6 in a temperature-controlled room (22 ⁇ 2°C) with a 12-hour light/ dark cycle and were fed ad libitum water and a standard rodent diet (Harlan Teklad diet 2018i). All animals were uniquely identifiable by ear tag number.
  • Freshly harvested cell suspensions were subcutaneously inoculated ( ⁇ ,) on the right flank with 5x10 6 HI 975 or A431 cells in PBS.
  • Mean tumour diameter was averaged from the longest diameter (length) multiplied by the perpendicular measurement (width).
  • tumours Treatment was initiated when the tumours reached a volume of approximately 250 mm 3 , as determined by calliper measurement. All drugs were given by intraperitoneal injection at dosing volumes of 10-20 ml/kg. Mice were dosed at the MTD over a q3dx4, q5dx4 or q7dx4 schedule with tumour growth measured by callipers every 3-5 days over the 30-day duration of the study. Mice were culled if they developed signs of toxicity or if bodyweight loss exceeded 20% of starting weight. All animal experiments followed protocols approved by the Animal Ethics Committee of The University of Aucldand.
  • tumour bearing mice were assigned randomly to treatment groups when tumour diameter reached treatment size. Animals were rejected if xenografts show evidence of: (i) attachment to underlying muscle (due to risk of local invasion), (ii) signs of ulceration, or (iii) indolent tumour growth. Drug administration begins on the day of assignment
  • tumour size and body weights were measured regularly. Animals were culled if (i) the average diameter of the tumour exceeds 5 mm (survival endpoint), (ii) body weight loss exceeds 20% of pre-treatment value, (iii) there is evidence of prolonged or excessive morbidity, or (iv) tumour ulceration occurred. The experiment was terminated at day 21 (A431 tumours) or day 30 (HI 975 tumours) after treatment initiation.
  • RTV 4 The time for individual tumours to increase in volume by 4 fold relative to treatment day-1 (RTV 4 ) was recorded.
  • the median RTV 4 is calculated for each group and the difference in RTV 4 between control and treatment groups is described as the Tumour Growth Delay (TGD) in days.
  • TGD Tumour Growth Delay
  • Kaplan-Meier plots were constructed and median survival was calculated ⁇ 50 ).
  • Weight loss nadirs time independent maxima were recorded for each treatment group. Any signs of treatment related morbidity were documented. Acceptable toxicity was defined as no mean group weight loss of over 15% during the test, no individual weight loss over 20% and no individual weight loss over 10% in any 24h period. All unscheduled deaths were recorded. R ⁇ esiilts
  • tumour growth delay was particularly significant for compounds 2-9 when administered at q3dx4 (P ⁇ 0.05, log-rank test) in both H1975 ( Figures 1-3) and A431 ( Figure 4) tumours. There was only minor bodyweight loss across all groups, although there was 1 death following treatment with compounds 5 and 7 in HI 975 xenograft mice (Table 4).
  • Prodrug 17 was tested at multiple dosing schedules: q3dx4, q5dx4 and q7dx4 at its q3dx4 MTD. At all 3 dosing schedules, compound 17 significantly delayed tumour growth in HI 975 tumours compared to controls ( Figure 11). There were no statistically significant differences in tumour growth between die 3 dosing schedules. Bod weight loss was greatest after q3dx4 dosing and minitnal after q7dx4 dosing (Table 6). However, 1 death was observed in the q7dx4 treatment group. It is not clear if this death was drug-related or not.
  • Compound 17 was also administered at q3dx4 and q5dx4 at its q3dx4 MTD to A431 xenograft mice, with both dosing schedules causing similar delays in A431 tumour growth and similar losses in animal bodyweight (Table 6).
  • the in vitro and in vivo activity data illustrate the effectiveness of the compounds of the invention as kinase inhibitors.
  • the compounds are therefore suitable for use in kinase-inliibitory therapy. This is particularly the case with the reductive prodrugs and cancer therapy as tumours commonly have hypoxic regions.
  • the prodrugs are reduced under hypoxia to release the parent kinase inhibitor and produce a tumour-targeted effect.
  • Prodrugs 15, 17 and 18 are considered particularly promising therapeutic candidates. While the present invention is broadly as described above, those persons skilled in the art will appreciate that the specific description is illustrative only and that variations may be made without departing from the invention as defined in the following claims.

Abstract

La présente invention porte sur des composés de pyrido[3,4-d]pyrimidine de formule (I) appropriés comme inhibiteurs de kinases, sur des inhibiteurs de kinases sous forme de promédicaments, sur leur utilisation dans des compositions et médicaments qui sont appropriés pour le traitement de maladies liées à une activité de kinases telles que des cancers.
PCT/NZ2010/000174 2009-09-02 2010-09-02 Inhibiteurs de kinases, formes promédicaments de ceux-ci et leur utilisation en thérapie WO2011028135A1 (fr)

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US13/390,398 US9101632B2 (en) 2009-09-02 2010-09-02 Kinase inhibitors, prodrug forms thereof and their use in therapy
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CA2771016A CA2771016A1 (fr) 2009-09-02 2010-09-02 Inhibiteurs de kinases, formes promedicaments de ceux-ci et leur utilisation en therapie
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AU2016344040B2 (en) 2015-11-01 2020-12-10 The Regents Of The University Of Colorado, A Body Corporate Wee 1 kinase inhibitors and methods of making and using the same
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US9101632B2 (en) 2015-08-11
RU2012112396A (ru) 2013-10-10
RU2568639C2 (ru) 2015-11-20
EP2473509A4 (fr) 2013-04-17
BR112012004720B1 (pt) 2021-06-29
AU2010290199B2 (en) 2016-10-27
NZ598144A (en) 2014-01-31
US20160031877A1 (en) 2016-02-04
IL218182B (en) 2018-03-29
AU2010290199A1 (en) 2012-03-01
JP2013503859A (ja) 2013-02-04
ZA201200994B (en) 2012-10-31
EP2473509A1 (fr) 2012-07-11
CA2771016A1 (fr) 2011-03-10
JP5925680B2 (ja) 2016-05-25
CN102574846A (zh) 2012-07-11
CN104496982A (zh) 2015-04-08
MX336332B (es) 2016-01-15
CN102574846B (zh) 2014-12-03
IL218182A0 (en) 2012-07-31
MX2012002327A (es) 2012-04-10
JP2016094432A (ja) 2016-05-26
KR20120076350A (ko) 2012-07-09
BR112012004720A2 (pt) 2016-03-15
US20120202832A1 (en) 2012-08-09

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