WO2008034337A1

WO2008034337A1 - Excrement and urine treatment apparatus for an ecological flush-free toilet

Info

Publication number: WO2008034337A1
Application number: PCT/CN2007/002591
Authority: WO
Inventors: Longji Wei; Ling Zhang
Original assignee: Longji Wei; Ling Zhang
Priority date: 2006-09-05
Filing date: 2007-08-28
Publication date: 2008-03-27

Abstract

An ecological flush-free toilet and an excrement and urine treatment apparatus and a microbial agent for the ecological flush-free toilet, the excrement and urine treatment apparatus includes a temporarily storing case (1), a scraping means (2), a ferment case (3), and a mechanism gearing (4). The temporarily storing case (1) has an opening (11) on its upper portion corresponding to the bottom of a squatting pan(5). The temporarily storing case (1) is communicated with the ferment case (3). The excrement and urine in the temporarily storing case (1) are pushed into the ferment case (3) by the scraping means (2). The microbial agent is held in the ferment case (3). There is a stirrer (31) coupled to the mechanism gearing (4) inside the ferment case (3). A heating means (32) is disposed at the outside bottom of the ferment case (3).

Description

用于生态免冲水厕所的粪尿处理设备技术领域 Excrement treatment equipment for ecological flush-free toilets

本发明属于卫生设备领域，涉及一种用于生态免冲水厕所的粪尿处理设备和包括其的生态免冲水厕所以及其中所使用的微生物菌剂。背景技术 - 现有的免冲水厕所主要有两种形式：打包式免冲水厕所和生态免冲水厕所。打包式免冲水厕所需要耗费包装材料（如塑料袋），不但有较高的使用成本、清运成本，而且由于塑料制品的难降解性，更带来了二次污染；另外，在严寒天气中粪袋容易冻结，低温适应性差，限制了适用的地区。 The present invention belongs to the field of sanitary equipment, and relates to an excreta treatment apparatus for an ecological flush-free toilet and an ecological flush-free toilet including the same and a microbial agent used therein. BACKGROUND OF THE INVENTION - Existing flush-free toilets come in two main forms: packaged flush-free toilets and ecological flush-free toilets. Packed flush-free toilets require packaging materials (such as plastic bags), which not only have higher cost of use, cleaning and transportation costs, but also cause secondary pollution due to the difficult degradation of plastic products. In addition, in cold weather The medium dung bag is easy to freeze, and the low temperature adaptability is poor, which limits the applicable area.

而生态免冲水厕所是通过好氧微生物的生化降解作用将人粪尿就地进行资源化、无害化和减量化处理，克服了打包式免冲水厕所的缺陷。目前已经有不少配置不同、结构各异的生态免冲水厕所问世了，例如，中国专利 CN1189253C以及中国专利或专利申请 CN1773034A, CN2761126Y等公开的生态免冲水厕所，除了安置在固定场所，它们还可广泛应用到列车、汽车等移动场所（如，可参见中国专利 CN2803845Y、 CN2801575Y、 CN2838507Y) o 然而，这些现有的生态免冲水厕所中的粪尿处理设备（在现有技术中有时也被称为生化反应器、主机等）中的发酵容器与蹲便器底部直接连通，如厕人很容易直接看见并有可能接触到发酵容器内部混合有微生物菌剂的粪尿降解物以及搅拌器等内部设备。尽管在微生物菌剂的作用下已经大大改善了气味，但是这也给如厕人视觉感官上带来了不舒服的感觉；其次，当有如厕人进入厕所后，活动挡板打开时，为了安全起见，一般要关闭搅拌器，如果如厕频度高的话，长期关闭会很不利于粪尿和微生物菌剂混合和通气，影响微生物对粪尿的降解效率；另外，尤其是儿童有时会出于好奇而将手伸入发酵容器内部，而边缘比较尖锐的搅拌器即使在停止运转时也比较容易造成危险。 The ecological flush-free toilet is a resource-based, harmless and reduced treatment of human excrement by the biodegradation of aerobic microorganisms, which overcomes the defects of the packaged flush-free toilet. At present, there are many ecological flush-free toilets with different configurations and different structures. For example, the Chinese patent CN1189253C and the Chinese patent or patent application CN1773034A, CN2761126Y, etc. disclose ecological flush toilets, except for being placed in fixed places. It can also be widely applied to mobile sites such as trains and automobiles (for example, see Chinese patents CN2803845Y, CN2801575Y, CN2838507Y). However, these existing ecological flush-free toilets are also used in the prior art. The fermentation vessel in the bioreactor, the main engine, etc. is directly connected to the bottom of the squatting pot. The toilet person can easily see and directly contact the feces and urine degradants mixed with the microbial agent inside the fermentation vessel, and the stirrer. Internal equipment. Although the odor has been greatly improved under the action of the microbial agent, it also brings an uncomfortable feeling to the visual sense of the toilet person. Secondly, when the toilet person enters the toilet, the movable baffle is opened for safety. For example, it is generally necessary to turn off the agitator. If the frequency of toileting is high, long-term closure will be very detrimental to the mixing and aeration of feces and microbial agents, affecting the degradation efficiency of microorganisms on excreta; in addition, especially children sometimes come out Curious and put your hand inside the fermentation vessel, and the sharper edge of the agitator is more likely to be dangerous even when it is stopped.

为此，我们设计了一种新的用于生态免冲水厕所的粪尿处理设备，其增加了能够在如厕时暂时储存爽尿的暂时贮粪容器和没有可触及的尖锐边缘的刮动装置，使得包括了该设备的生态免冲水厕所不但保留了现有生态免冲水厕所臭味小、低温适应性好、压缩了粪便、尿液的体积从而容易清运的优点，更克服了现有技术中视觉感差、降解效率高、容易发生危险的缺点，更具有推广应用的前景。 To this end, we have devised a new excreta treatment device for ecological flush-free toilets, which adds a temporary septic container that can temporarily store fresh urine when going to the toilet and a scratch with no sharp edges that are accessible. The device makes the ecological flush-free toilet including the equipment not only retains the advantages of the existing ecological flush-free toilet, such as low odor, good low temperature adaptability, compressed manure and urine volume, and is easy to clear and transport, and overcomes the advantages. In the prior art, the disadvantages of poor visual perception, high degradation efficiency, and danger are likely to occur, and the prospect of popularization and application is further enhanced.

另外，像上述中国专利 CN1189253C等公开的生态免冲水厕所为了处理粪尿，其中所使用的生化填料中所含的菌种多达几十种，构成成分复杂，无法取得稳定的、重复性好的处理效果，更不容易控制安全性和产品质量。为此，本发明人在现有技术的基础上，出人意料地使用了菌种安全可靠、成分明确、数量较少的微生物菌剂，由此构成的厕所能够克服上述现有技术的缺陷，粪尿处理质量稳定、重复性好，更便于加强产品质量控制。发明内容 In addition, the ecological flush-free toilet disclosed in the above-mentioned Chinese patent CN1189253C, etc., is used for treating excrement. There are dozens of strains contained in the biochemical fillers, and the composition is complicated, and it is impossible to obtain a stable and reproducible treatment effect, and it is not easy to control safety and product quality. For this reason, on the basis of the prior art, the inventors have unexpectedly used microbial agents which are safe, reliable, well-defined and small in number, and the toilet thus constructed can overcome the above-mentioned drawbacks of the prior art, excrement The processing quality is stable and reproducible, and it is easier to strengthen product quality control. Summary of the invention

本发明的目的在于提供一种用于生态免冲水厕所的粪尿处理设备，其在现有生态免冲水厕所的基础上增加了能够暂时储存粪尿的暂时贮粪容器和没有可触及的尖锐边缘的刮动装置，并优选使用特定的微生物菌剂，从而使得包括了该设备的生态免冲水厕所克服了现有技术的上述缺点。本发明的目的还在于提供包括该设备的生态免冲水厕所和相应微生物菌剂。 The object of the present invention is to provide an excrement treatment device for an ecological flush-free toilet, which adds a temporary storage container capable of temporarily storing excrement and urine and has no accessibility on the basis of the existing ecological flush-free toilet. A sharp edged scraping device, and preferably a specific microbial inoculum, such that the ecological flush-free toilet including the device overcomes the above-discussed shortcomings of the prior art. It is also an object of the present invention to provide an ecological flush-free toilet and corresponding microbial agent comprising the device.

具体而言，在第一个方面，本发明提供了一种用于生态免冲水厕所的粪尿处理设备，包括暂时贮粪容器（1 )、刮动装置（2)、发酵容器（3 )和机械传动装置（4)，其特征在于，暂时贮粪容器（1 )上部带有开口（11 )，其与蹲便器（5 )底部的活动挡板（51 )相对应；暂时贮粪容器（1 ) 与发酵容器（3 )连通，通过刮动装置（2) 推动而可将暂时贮粪容器（1 ) 内的粪尿推入发酵容器（3 ); 发酵容器（3 ) 内装有微生物菌剂；发酵容器（3 ) 内有与机械传动装置（4) 联接的搅拌器（31 ) ; 而且发酵容器（3 ) 外底部设有加热装置 (32)。 Specifically, in a first aspect, the present invention provides an excreta processing apparatus for an ecological flush-free toilet, comprising a temporary storage container (1), a scraping device (2), and a fermentation container (3) And a mechanical transmission device (4), characterized in that the upper portion of the temporary storage container (1) has an opening (11) corresponding to the movable baffle (51) at the bottom of the squatting device (5); 1) communicating with the fermentation vessel (3), pushing the excrement in the temporary storage container (1) into the fermentation vessel (3) by pushing the scraping device (2); the fermentation vessel (3) is filled with the microbial agent The fermentation vessel (3) has a stirrer (31) coupled to the mechanical transmission (4); and the outer bottom of the fermentation vessel (3) is provided with a heating device (32).

在该粪尿处理设备中，机械传动装置（4)联接带动发酵容器（3 ) 内的搅拌器（31 ) 进行搅拌。为了方便使用起见，该设备还可包括电控装置，所述电控装置能控制刮动装置 In the excrement processing apparatus, the mechanical transmission (4) is coupled to agitator (31) in the fermentation vessel (3) for agitation. For convenience of use, the device may further include an electronic control device capable of controlling the scraping device

(2)、机械传动装置（4)和加热装置（32)。所述电控装置可以自动进行控制，如通过可编程的微处理芯片控制。优选该电控装置还可与免冲水厕所的蹲便室（100) 中的光电感应器（6)相连接，所述光电感应器（6)用于感应如厕者的活动。这样便可以根据光电感应器（6)感应如厕者活动的情况，自动运行该粪尿处理设备。 (2) Mechanical transmission (4) and heating device (32). The electronic control unit can be automatically controlled, such as by a programmable microprocessor chip. Preferably, the electronic control unit is also connectable to a photoconductor (6) in the toilet (100) of the flush-free toilet, the photoelectric sensor (6) being used to sense the activity of the toilet. In this way, the urinary treatment device can be automatically operated according to the condition in which the photoconductor (6) senses the movement of the occupant.

例如，在如厕者离开后将蹲便器（5) 的活动挡板（51 )关闭时，才允许开启刮动装置 For example, it is allowed to open the scraping device when the movable baffle (51) of the squatting pan (5) is closed after the toilet person leaves.

(2)，将暂时贮粪容器（1 ) 内的粪尿推入发酵容器（3 )，将粪尿及微生物菌剂在发酵容器（3 ) 内进行搅拌混合，然后使刮动装置（2) 复位。电控装置还可以通过其中的微电脑装置根据微生物的性质和 /或所连接的感应器感应到的粪尿量、如厕频度、温度和 /或湿度等因素，来控制机械传动装置（4) 从而带动搅拌器（31 )运转并控制加热装置（32) 的运行，提高疏松度易增加通气，控制发酵容器内粪尿的水分，持微生物菌种的最佳降解活性的温度、湿度和含氧量，从而实现粪尿的得就地减量化，并保。由于设置了暂时贮粪容器（1 )，因此电控装置控制的搅拌器（31 )可以不必在活动挡板 (51 ) 打开时强制停止运行，使得微生物菌种降解处理的过程更为顺畅。 (2) Pushing the excrement in the temporary storage container (1) into the fermentation container (3), stirring and mixing the excrement and the microbial agent in the fermentation container (3), and then making the scraping device (2) Reset. The electronic control device can also control the mechanical transmission device by means of the micro-computer device according to the nature of the microorganism and/or the amount of fecal urine, toilet frequency, temperature and/or humidity sensed by the connected sensor (4) Thereby driving the agitator (31) to operate and controlling the operation of the heating device (32), increasing the degree of looseness, increasing the ventilation, controlling the moisture of the excrement in the fermentation vessel, and maintaining the optimal degradation activity of the microbial species, temperature, humidity and oxygenation. The amount, so that the depletion of the excrement can be achieved in situ. Due to the provision of temporary storage capacity The (1), therefore, the agitator (31) controlled by the electronic control device can be forced to stop running when the movable baffle (51) is opened, so that the process of degradation of the microbial strain is smoother.

优选在该粪尿处理设备中，机械传动装置（4)、搅拌器（31 )和加热装置（32) 分别可以是中国专利或专利申请 CN1773034A或 CN2761126Y中所述的相应机械传动装置、搅拌器和加热装置。在一个具体的实施方案实例中，所述搅拌器为单搅笼、双搅笼或多搅笼结构，其搅笼轴上联接有搅笼，搅笼为螺旋形，通过搅拌杆与搅笼轴联接，搅笼轴两端设置在发酵容器的前后壁上，搅笼轴一端安装有通孔滑动轴承，另一端安装有盲孔滑动轴承；所述的加热装置包括加热保护罩和加热元件，加热保护罩联接在发酵容器底部的外侧壁上，加热保护罩内设置有加热元件，加热元件迂回排列并由电控装置控制；和 /或，机械传动装置可以包括电机底座、单列链轮、链条、双列链轮、减速机、电机,、调整螺栓。在上电机底座通过调整螺栓安装有减速机和电机，减速机的输出轴上安装有双列链轮，双列链轮连动两链条，两链条分别连动两单列链轮，两单列链轮分别安装在两搅拌器的两搅笼轴上。 Preferably, in the excrement processing apparatus, the mechanical transmission (4), the agitator (31) and the heating device (32) may respectively be corresponding mechanical transmissions, agitators and the like described in Chinese Patent or Patent Application No. CN1773034A or CN2761126Y. heating equipment. In a specific embodiment example, the agitator is a single stir cage, a double stir cage or a multi-stirred cage structure, the stirrer shaft is coupled with a stirrer, the stirrer is spiral, and the stirrer shaft and the stirrer shaft are Coupling, both ends of the stirring cage shaft are arranged on the front and rear walls of the fermentation vessel, one end of the stirring cage shaft is provided with a through-hole sliding bearing, and the other end is equipped with a blind hole sliding bearing; the heating device comprises a heating protective cover and a heating element, heating The protective cover is coupled to the outer side wall of the bottom of the fermentation vessel, and the heating cover is provided with heating elements, the heating elements are arranged in a roundabout manner and controlled by the electronic control device; and/or the mechanical transmission device may comprise a motor base, a single row of sprocket wheels, a chain, Double row sprocket, reducer, motor, and adjustment bolts. A reducer and a motor are mounted on the upper motor base through adjusting bolts. The output shaft of the reducer is equipped with a double-row sprocket, the double-row sprocket is linked with two chains, and the two chains respectively link two single-row sprocket, two single-row sprocket Installed on the two stirrer shafts of the two agitators.

发酵容器（3 ) 可以带有换气设备，如排气管和 /或排气风扇，从而为发酵容器（3 ) 内部换气并保持湿度，优选该换气设备也受前述电控装置控制。另外，发酵容器（3 ) 上可以有能开闭的门，这样可以在需要时，清理掉发酵容器（3 ) 内的经微生物菌剂处理过的粪尿，也可添加新的微生物菌剂。另外，在暂时贮粪容器（1 ) 也可以带有可以开闭的门，可以在需要时，方便清理暂时贮粪容器（1 ) 角落里遗留的污垢。这对本领域技术人员来说是显而易见的。 The fermentation vessel (3) may be provided with a ventilating device, such as an exhaust pipe and/or an exhaust fan, to ventilate the interior of the fermentation vessel (3) and maintain humidity. Preferably, the ventilating device is also controlled by the aforementioned electronic control device. In addition, the fermentation vessel (3) may have a door that can be opened and closed, so that the microbial-treated excrement in the fermentation vessel (3) can be cleaned up when necessary, and a new microbial agent can be added. In addition, the temporary storage tank (1) may also have a door that can be opened and closed, and it is convenient to clean the dirt left in the corner of the temporary storage container (1) when necessary. This will be apparent to those skilled in the art.

在该粪尿处理设备中，所述刮动装置（2)优选可以通过电动机（21 )带动来进行推进或拉出（拉出在本文中也称为复位），其中可以使用各种传动装置。例如，所述刮动装置 (2)通过齿轮（23 )传动来进行推进或拉出。这在图 1和图 4中有详细地图示，电动机 (21 )通过带动齿轮（23 )而带动刮动装置（2)推进或拉出。所述刮动装置（2) 也可以通过连杆（22)传动来进行推进或拉出。特定的实例如图 5和图 6所示，它们分别呈复位和推进状态。优选刮动装置（2)在暂时贮粪容器（1 ) 内的部分没有可触及的尖锐边缘。 In the urethral treatment device, the squeezing device (2) can preferably be propelled or pulled (pulled out, also referred to herein as a reset) by the motor (21), wherein various transmissions can be used. For example, the scraping device (2) is driven or pulled out by gearing (23). This is illustrated in detail in Figures 1 and 4, in which the motor (21) drives the gear unit (23) to drive or pull the scraping device (2). The scraping device (2) can also be driven or pulled out by a connecting rod (22). Specific examples are shown in Figures 5 and 6, which are in reset and boost states, respectively. Preferably, the portion of the scraping device (2) in the temporary storage container (1) has no sharp edges that are accessible.

为了取得安全可靠、质量稳定、重复性好的微生物降解效果，也为了便于加强产品质量的控制，优选该粪尿处理设备中装有的微生物菌剂含有芽孢杆菌并含有酿酒酵母和 /或荧光假单胞菌。更优选，所述微生物菌剂基本由一种或多种芽孢杆菌和选自酿酒酵母和荧光假单胞菌的一种或多种菌株组成。其中，芽孢杆菌包括但不限于巨大芽孢杆菌、枯草芽孢杆菌、球形芽孢杆菌、环状芽孢杆菌和地衣芽孢杆菌。本文中使用的术语"基本由…组成" 表示该术语所列举的菌占微生物菌剂所含的菌总量的绝大多数，通常占到 90%以上，更优选实质上不添加其他菌。本发明使用的菌剂中的菌株远远少于中国专利 CN1189253C等公开的菌剂的，但是它配合本发明第一方面的粪尿处理设备使用仍旧能对粪尿取得良好的除臭、减量的效果。另外，菌剂中所含的上述每两种菌（株）之间的重量比优选都不超出 1 : 0.1-10, 也就是说，菌剂中含量最大的菌（株）的量不超过其中含量最小的菌（株）的 10 倍，最优选基本上是等量的。 In order to obtain a safe, reliable, stable and reproducible microbial degradation effect, and also to facilitate the control of product quality, it is preferred that the microbial agent contained in the excrement treatment device contains Bacillus and contains Saccharomyces cerevisiae and/or fluorescent fake Monocytogenes. More preferably, the microbial agent consists essentially of one or more strains of Bacillus and one or more strains selected from the group consisting of Saccharomyces cerevisiae and Pseudomonas fluorescens. Among them, Bacillus includes, but is not limited to, Bacillus megaterium, Bacillus subtilis, Bacillus sphaericus, Bacillus circulans, and Bacillus licheniformis. The term "consisting essentially of" as used herein means that the bacteria listed in the term account for the vast majority of the total amount of bacteria contained in the microbial agent, usually accounting for more than 90%, preferably The selection does not substantially add other bacteria. The strain used in the microbial agent used in the present invention is far less than the microbial agent disclosed in Chinese Patent No. CN1189253C, but it can still achieve good deodorization and reduction of excrement in the use of the excrement treatment device of the first aspect of the present invention. Effect. In addition, the weight ratio between each of the above two strains contained in the microbial agent is preferably not more than 1: 0.1-10, that is, the amount of the most abundant fungus in the microbial agent is not more than It is 10 times, and most preferably substantially equal, of the strain having the smallest content.

更优选地，所述芽孢杆菌优选是选自保藏号为 CGMCC No.1724 的巨大芽孢杆菌 {Bacillus megaterium ) ZL-1010株、保藏号为 CGMCC No.1725的枯草芽孢杆菌 (Bacillus subtilis) ZL-1007株、保藏号为 CGMCC No.1726的球形芽孢杆菌 ί Bacillus sphaericus) ZL-1008株、保藏号为 CGMCC No.1727的环状芽孢杆菌 ί Bacillus circulam ZL-1009株、保藏号为 CGMCC No. 2020的地衣芽孢杆菌 Bacillus licheniformis) ZL-1013株中的一种或多种，所述酿酒酵母优选是保藏号为 CGMCC No.2018 的酿酒酵母 Saccharomyces cerevisiae ) ZL-1011株，和 /或所述荧光假单胞菌优选是保藏号为 CGMCC No. 2021的荧光假单胞菌 iPseudomonas fluoresc s ZL-1014株。上述菌株保藏于中国微生物菌种保藏管理委员会普通微生物中心（中国，北京），其中 ZL-1010株、 ZL-1007株、 ZL-1008株和 ZL-1009株的保藏日期均为 2006年 5月 30日； ZL-1013株、 ZL-1011株和 ZL-1014株的保藏日期均为 2007年 4月 25日。在本发明的一个具体实施方式中，一种特定的菌剂配方（菌剂 A)包括巨大芽孢杆菌 ZL-1010株、枯草芽孢杆菌 ZL-1007株、球形芽孢杆菌 ZL-1008 株、地衣芽孢杆菌 ZL-1013和酿酒酵母 ZL-1011株。在另一个具体实施方式中，一种特定的菌剂配方（菌剂 B )包括枯草芽孢杆菌 ZL-1007株、地衣芽孢杆菌 ZL-1013株和荧光假单胞菌 ZL-1014株。以上菌剂委托上海市环境监测中心（中国，上海）进行环境安全性评价，没有发现明显的环境安全性问题，因此可以安全地推广应用，而且成分明确，容易进行质量控制。 More preferably, the Bacillus is preferably selected from the group consisting of Bacillus megaterium ZL-1010 strain deposited under the accession number CGMCC No. 1724, and Bacillus subtilis ZL-1007 deposited under the accession number CGMCC No. 1725. Strain Bacillus sp. sphaericus ZL-1008 with CGMCC No. 1726, Bacillus circumatum 340 with strain CGMCC No. 1727, and CGMCC No. 2020 One or more strains of Bacillus licheniformis) ZL-1013, preferably Saccharomyces cerevisiae ZL-1011 strain deposited under the accession number CGMCC No. 2018, and/or the fluorescent pseudonym The bacterium is preferably the Pseudomonas fluorescens iPseudomonas fluoresc s ZL-1014 strain deposited under the number CGMCC No. 2021. The above strains were deposited in the General Microbiology Center of the China Microbial Culture Collection Management Committee (Beijing, China), and the dates of ZL-1010, ZL-1007, ZL-1008 and ZL-1009 were all deposited on May 30, 2006. The date of storage of ZL-1013 strain, ZL-1011 strain and ZL-1014 strain was April 25, 2007. In a specific embodiment of the present invention, a specific microbial formulation (bacteria A) includes Bacillus megaterium ZL-1010 strain, Bacillus subtilis ZL-1007 strain, Bacillus sphaericus ZL-1008 strain, Bacillus licheniformis ZL-1013 and Saccharomyces cerevisiae ZL-1011 strain. In another embodiment, a specific bacterial formulation (bacterial agent B) comprises Bacillus subtilis ZL-1007 strain, Bacillus licheniformis ZL-1013 strain, and Pseudomonas fluorescens ZL-1014 strain. The above-mentioned fungicides were commissioned by the Shanghai Environmental Monitoring Center (China, Shanghai) for environmental safety evaluation. No obvious environmental safety problems were found, so it was safe to promote the application, and the ingredients were clear and easy to control quality.

另外，酿酒酵母 ZL-1011株具有絮凝性，我们的研究还明确了酿酒酵母 ZL-1011株的分子分类背景，其具有不同于现有已知菌株的如下 26S rRNA基因序列（Seq ID No. l ) : ATCCTTGGAC TTACGTCGCA GTCCTCAGTC CCAGCTGGCA GTATTCCCAC AGGCTATAAT ACTTACCGAG GCAAGCTACA TTCCTATGGA TTTATCCTGC CACCAAAACT GATGCTGGCC CAGTGAAATG CGAGATTCCC CTACCCACAA GGAGCAGAGG GCACAAAACA CCATGTCTGA TCAAATGCCC TTCCCTTTCA ACAATTTCAC GTACTTTTTC ACTCTCTTTT CAAAGTTCTT In addition, Saccharomyces cerevisiae ZL-1011 strain has flocculation, and our study also clarified the molecular classification background of S. cerevisiae ZL-1011 strain, which has the following 26S rRNA gene sequence different from the existing known strains (Seq ID No. l ) : ATCCTTGGAC TTACGTCGCA GTCCTCAGTC CCAGCTGGCA GTATTCCCAC AGGCTATAAT ACTTACCGAG GCAAGCTACA TTCCTATGGA TTTATCCTGC CACCAAAACT GATGCTGGCC CAGTGAAATG CGAGATTCCC CTACCCACAA GGAGCAGAGG GCACAAAACA CCATGTCTGA TCAAATGCCC TTCCCTTTCA ACAATTTCAC GTACTTTTTC ACTCTCTTTT CAAAGTTCTT

TTCATCTTTC CATCACTGTA CTTGTTCGCT ATCGGTCTCT CGCCAATATT TAGCTTTAGA TGGAATTTAC CACCCACTTA GAGCTGCATT CCCAAACAAC TCGACTCTTC GAAGGCACTT TACAAAGAAC ' CGCACTCCTC GCCACACGGG ATTCTCACCC TCTATGACGT CCTGTTCCAA GGAACATAGA CAAGGAACGG CCCCAAAGTT GCCCTCTCCA AATTACAACT CGGGCACCGA AGGTACCAGA TTTCAAATTT GAGCTTTTGC CGCTTCACTC GCCGTTACTATTCATCTTTC CATCACTGTA CTTGTTCGCT ATCGGTCTCT CGCCAATATT TAGCTTTAGA TGGAATTTAC CACCCACTTA GAGCTGCATT CCCAAACAAC TCGACTCTTC GAAGGCACTT TACAAAGAAC ' CGCACTCCTC GCCACACGGG ATTCTCACCC TCTATGACGT CCTGTTCCAA GGAACATAGA CAAGGAACGG CCCCAAAGTT GCCCTCTCCA AATTACAACT CGGGCACCGA AGGTACCAGA TTTCAAATTT GAGCTTTTGC CGCTTCACTC GCCGTTACTA

AGGCAATCCC GGTTGGTTTC TTTCCTCGGC T。 AGGCAATCCC GGTTGGTTTC TTTCCTCGGC T.

因此，可利用常规的 PCR、核酸杂交等技术（如可参见《分子克隆实验指南（第三版)》，科学出版社，北京， 2002年）定性或定量分析酿酒酵母 ZL-1011株，从而可利用该菌株作为指标，评估出微生物菌剂中菌株的含量，从而可以判断微生物菌剂（或粪尿处理设备）总有效成分的存活率，有效监控产品质量。 Therefore, conventional techniques such as PCR and nucleic acid hybridization can be utilized (for example, see "Molecular Cloning Experimental Guide (Third Edition)", Science Press, Beijing, 2002) for qualitative or quantitative analysis of Saccharomyces cerevisiae ZL-1011 strain, thereby Using the strain as an indicator, the content of the strain in the microbial agent is evaluated, so that the survival rate of the total active ingredient of the microbial agent (or excrement treatment equipment) can be judged, and the product quality can be effectively monitored.

优选在该粪尿处理设备中，所述微生物菌剂通常还包括适当的填料，例如它可以含有木屑、锯末、刨花、蔗渣、农作物秸秆或壳中的一种或多种。填料颗粒中至少有 90%粉碎至不大于 0.5cm*0.5cm的大小，优选至少有 90%粉碎至不大于 0.1cm*0.1cm的大小。填料与粪尿混合后能够增加疏松度以促进通气，而且能够为微生物菌剂赋形，便于添加入该设备的发酵容器（3 ) 中。从重量上来说，微生物菌剂中填料的量通常占绝大多数，通常占总量的 95%以上，如 98%、 99%或 99. 9%。加入的微生物菌剂的体积不溢出到暂时贮粪容器（1 ) 内，通常为发酵容器（3 ) 的三分之一至六分之五，优选微生物菌剂的体积占发酵容器（3 ) 的容积的四分之三。 Preferably, in the excreta treating apparatus, the microbial agent usually further comprises a suitable filler, for example, it may contain one or more of wood chips, sawdust, shavings, bagasse, crop straw or shell. At least 90% of the filler particles are pulverized to a size of not more than 0.5 cm * 0.5 cm, preferably at least 90% pulverized to a size of not more than 0.1 cm * 0.1 cm. The filler is mixed with the excrement to increase the porosity to promote ventilation, and can be shaped into a microbial agent for easy addition to the fermentation vessel (3) of the device. In terms of weight, the amount of filler in the microbial agent usually accounts for the majority, usually accounting for more than 95% of the total, such as 98%, 99% or 99.9%. The volume of the added microbial agent does not overflow into the temporary storage container (1), usually from one-third to five-fifth of the fermentation container (3), and preferably the volume of the microbial agent accounts for the fermentation container (3). Three-quarters of the volume.

在粪尿处理设备中，上述菌剂的用量可以根据要处理的如厕者的日粪尿总量（也可以表示为如厕者人数）来确定，本发明的菌剂与平均每日如厕者人数的关系通常可以达到 100 人次 /30公斤菌剂，优选为小于 80人次 /30公斤菌剂，如在本发明的具体实施方式中为约 50人次 /30公斤菌剂，可以连续使用 3个月或更长而无需更换菌剂。 In the excreta processing apparatus, the amount of the above-mentioned microbial agent can be determined according to the total amount of daily feces and urine (which can also be expressed as the number of toilets) of the toilet person to be treated, and the microbial agent of the present invention and the average daily toilet The relationship between the number of people can usually reach 100 person/30 kg of bacteria, preferably less than 80 times / 30 kg of bacteria, as in the embodiment of the invention is about 50 times / 30 kg of bacteria, can be used continuously for 3 Months or longer without the need to change the bacteria.

在一个单独的方面，本发明可以提供上述微生物菌剂。本发明第一个方面的包括该微生物菌剂的粪尿处理设备优选用于处理粪便。 In a separate aspect, the present invention can provide the above microbial agents. The excreta treating apparatus comprising the microbial agent of the first aspect of the invention is preferably used for treating feces.

在另一个方面，本发明还提供了一种生态免冲水厕所，包括设于基面（7)上的蹲便室 ( 100)和设于基面（7 )下的处理室（200)，其特征在于，蹲便室（100) 安装有蹲便器 (5)，所述蹲便器（5 ) 安装在基面（7) 上面；而且，处理室（200) 内安装有本文上述的粪尿处理设备，其中暂时贮粪容器（1 )上部带有开口（11 )，其与蹲便器（5 )底部的活动挡板 (51 )相对应。 In another aspect, the present invention also provides an ecological flush-free toilet comprising a squatting chamber (100) disposed on a base surface (7) and a processing chamber (200) disposed under the base surface (7). The utility model is characterized in that the squatting chamber (100) is equipped with a squatting pan (5), the squatting pan (5) is mounted on the base surface (7); and the treatment chamber (200) is installed with the excrement treatment described above. The device, wherein the temporary storage container (1) has an opening (11) at the upper portion corresponding to the movable baffle (51) at the bottom of the squatting pan (5).

优选在该生态免冲水厕所中，所述蹲便室（100) 中有光电感应器（6)，所述光电感应器（6)与所述粪尿处理设备中的电控装置相连接，而且其用于感应如厕者的活动。例如，当检测到有如厕者时，蹲便器（5 )底部的活动挡板（51 )打开，电控装置控制刮动装置 (2) 呈复位状态；当检测到如厕者离开时，蹲便器（5 ) 底部的活动挡板（51 ) 关闭，电控装置控制刮动装置（2) 将粪尿推入发酵容器（3 )，然后复位，并可在适当时控制机械传动装置（4)和加热装置（32)运行。 Preferably, in the ecological flush toilet, the squatting chamber (100) has a photoelectric sensor (6), and the photoelectric sensor (6) is connected to the electronic control device in the urinary processing device. And it is used to sense the activities of the toilet. For example, when a toilet is detected, the movable baffle (51) at the bottom of the squatting pan (5) is opened, the electric control device controls the striking device (2) to be in a reset state; when the toilet occupant is detected, the squat pan (5) The bottom flap (51) is closed, the electric control device controls the scraper (2) to push the excrement into the fermentation vessel (3), then reset, and control the machine when appropriate The transmission (4) and the heating device (32) operate.

另外，所述蹲便器（5 )底部的活动挡板（51 ) 的开合方式优选为水平移动式开合。此外，蹲便室（100) 还可以安装有旋启窗、门、小便池、洗手池、排气管和 /或排气风扇等设备，处理室（200)也可以安装有排气管和 /或排气风扇等设备，这些对于本领域普通技术人员来说都是显而易见的。 In addition, the opening and closing manner of the movable baffle (51) at the bottom of the squatting pan (5) is preferably a horizontally movable opening and closing. In addition, the toilet (100) can also be equipped with a swing window, a door, a urinal, a sink, an exhaust pipe and/or an exhaust fan. The processing chamber (200) can also be equipped with an exhaust pipe and/or Or equipment such as an exhaust fan, these will be apparent to those of ordinary skill in the art.

本发明中所用元件的材质对于本领域技术人员来说是是显而易见的，均可以通过市场购买，如暂时贮粪容器（1 )、发酵容器（3 ) 的材质可以是玻璃钢、不锈钢等，而常用的光电感应器（6)有市售的红外线感应器。为了便于理解，以下将通过具体的附图和实施例对本发明进行详细地描述。需要特别指出的是，具体实例和附图仅是为了说明，并不构成对本发明范围的限制。显然本领域的普通技术人员可以根据本文说明，在本发明的范围内对本发明做出各种各样的修正和改变，这些修正和改变也纳入本发明的范围内。另外，本发明引用了公幵文献（如特定的专利等），这些文献也是为了更清楚地描述本发明，它们的全文内容均纳入本发明进行参考，就好像它们的全文已经在本发明说明书中重复叙述过一样。附图说明 The materials of the components used in the present invention are obvious to those skilled in the art, and can be purchased by the market, for example, the temporary storage container (1), the fermentation container (3) can be made of fiberglass, stainless steel, etc. The photoelectric sensor (6) has a commercially available infrared sensor. For ease of understanding, the present invention will be described in detail below through the specific drawings and embodiments. It is to be understood that the specific examples and drawings are not to be construed as limiting. It is apparent that those skilled in the art can make various modifications and changes to the present invention within the scope of the present invention, and such modifications and changes are also included in the scope of the present invention. In addition, the present invention is hereby incorporated by reference in its entirety in its entirety in the entireties in the the the the the the the the the Repeat the same. DRAWINGS

图 1为本发明的用于免冲水厕所的粪尿处理设备的示意图，其使用了齿轮传动装置。图 2为本发明的免冲水厕所的部分结构的详细示意图。 BRIEF DESCRIPTION OF THE DRAWINGS Fig. 1 is a schematic view of a waste water treatment apparatus for a flush-free toilet according to the present invention, which uses a gear transmission. 2 is a detailed schematic view showing a part of the structure of the flush-free toilet of the present invention.

图 3为本发明的免冲水厕所的整体结构的示意图。 Figure 3 is a schematic view showing the overall structure of the flush-free toilet of the present invention.

图 4为图 1中 F所指向的齿轮传动接合处的放大图。 Figure 4 is an enlarged view of the geared joint pointed by F in Figure 1.

图 5为本发明的用于免冲水厕所的粪尿处理设备的示意图，其使用了连杆传动装置，呈复位状态。 Fig. 5 is a schematic view of the excrement treatment apparatus for a flush-free toilet according to the present invention, which uses a link transmission device in a reset state.

图 6为本发明的用于免冲水厕所的粪尿处理设备的示意图，其使用了连杆传动装置，呈推出状态。具体实施方式 Fig. 6 is a schematic view of the excrement processing apparatus for a flush-free toilet according to the present invention, which uses a link transmission device in a pushed-out state. detailed description

实施例 1 微生物菌剂的制备和安全性评价 Example 1 Preparation and safety evaluation of microbial agents

培养保藏号为 CGMCC No. 2018的酿酒酵母 Saccharomyces cerevisiae ZL-1011株所用的培养基配方：蛋白胨 10.0克、酵母提取物 1.0克、糖蜜 5.0克、水 1.0升，自然 pH， 115度灭菌 30分钟。以 25-28Ό摇床（200转 /分）振荡培养 36-48小时。取 1公斤灭菌后的木屑与发酵菌液混合均勾，于室温晾干，即为酵母菌菌剂。 Culture medium used for the cultivation of Saccharomyces cerevisiae ZL-1011 strain of CGMCC No. 2018: peptone 10.0 g, yeast extract 1.0 g, molasses 5.0 g, water 1.0 liter, natural pH, 115 degree sterilization for 30 minutes . Incubate for 36-48 hours with a 25-28 shaker (200 rpm). Take 1 kg after sterilization The wood chips are mixed with the fermentation broth and dried at room temperature, which is a yeast bacterium.

保藏号为 CGMCC No.1724的巨大芽孢杆菌 ZL-1010株、保藏号为 CGMCC No.1725 的枯草芽孢杆菌 ZL-1007株、保藏号为 CGMCC No.1726的球形芽孢杆菌 ZL-1008株、保藏号为 CGMCC No.1727的环状芽孢杆菌 ZL-1009株、保藏号为 CGMCC No. 2020的地衣芽孢杆菌 ZL-1013株、和保藏号为 CGMCC No. 2021的荧光假单胞菌 ZL-1014株这 6种菌株分别进行液体培养，所用培养基配方为：蛋白胨 10.0克、酵母提取物 1.0克、糖蜜 5.0 克、 NaC1 5克、水 1.0升，用 lM NaOH调 pH至 7.0-7.2， 115度灭菌 30分钟。其中各菌株的培养温度和培养时间略有差异，其中荧光假单胞菌 CGMCC No. 2021菌株在 30-32Ό 摇床（200转 /分）振荡培养 24-30小时，而另外 5种芽孢杆菌菌株在 35-37°C摇床（200转 /分）振荡培养 36-40小时。同样分别用 1kg灭菌后的木屑与发酵菌液混合均勾，于室温晾干，分别制备出仅包含单一菌种的菌剂。 The Bacillus megaterium ZL-1010 strain with the accession number CGMCC No.1724, the Bacillus subtilis ZL-1007 strain with the accession number CGMCC No.1725, and the Bacillus sphaericus ZL-1008 strain with the accession number CGMCC No.1726, the accession number Bacillus circulans ZL-1009 strain of CGMCC No. 1727, Bacillus licheniformis ZL-1013 strain deposited under CGMCC No. 2020, and Pseudomonas fluorescens ZL-1014 strain deposited under CGMCC No. 2021. The six strains were separately cultured in liquid. The medium used was: peptone 10.0 g, yeast extract 1.0 g, molasses 5.0 g, NaC1 5 g, water 1.0 liter, pH adjusted to 7.0-7.2 with 1 M NaOH, 115 degree sterilization 30 minutes. The culture temperature and culture time of each strain were slightly different, and the Pseudomonas fluorescens CGMCC No. 2021 strain was shake cultured for 30-30 hours on a 30-32 摇 shaker (200 rpm), while the other 5 Bacillus strains were cultured. The mixture was shaken at 35-37 ° C for shaking (200 rpm) for 36-40 hours. Similarly, 1 kg of the sterilized wood chips and the fermentation broth were separately mixed and dried at room temperature to prepare a microbial agent containing only a single strain.

然后，将上述制备好的 7种单一菌剂，按以下配方等量混合均匀，即构成复合菌剂：菌剂 A:巨大芽孢杆菌 ZL-1010株、枯草芽孢杆菌 ZL-1007株、球形芽孢杆菌 ZL-1008 株、地衣芽孢杆菌 ZL-1013和酿酒酵母 ZL-1011株。 Then, the seven kinds of single microbial preparations prepared above are uniformly mixed according to the following formulas to form a composite microbial agent: Bacterial agent A: Bacillus megaterium ZL-1010 strain, Bacillus subtilis ZL-1007 strain, Bacillus sphaericus ZL-1008 strain, Bacillus licheniformis ZL-1013 and Saccharomyces cerevisiae ZL-1011 strain.

菌剂 B:枯草芽孢杆菌 ZL-1007株、地衣芽孢杆菌 ZL-1013株和荧光假单胞菌 ZL-1014 株。 Bacterial agent B: Bacillus subtilis ZL-1007 strain, Bacillus licheniformis ZL-1013 strain and Pseudomonas fluorescens ZL-1014 strain.

委托上海市环境监测中心（中国，上海）对菌剂 A进行环境安全性评价，没有发现明显的环境安全性问题。具体而言，按照中国国家标准 GB7919-87 (《化妆品安全性评价程序和方法》)，经小鼠经口急性毒性试验发现无急性毒性，经小鼠骨髓嗜多染细胞微核试验发现不具有染色体畸变作用，经动物皮肤刺激试验发现无刺激性；按照《水和废水监测分析 · 方法》（第四版，中国国家环境保护总局， 2002年），经 Ames试验发现不具致突变性，经藻类急性毒性测试发现对藻类无明显的急性毒性作用，经藻类生态测试对自然水体的藻类生态不会产生明显的不利影响；按照中国国家标准 GB/T13267-91 (《水质物质对淡水鱼（斑马鱼）急性毒性测试方法》），经鱼类急性毒性测试发现无明显的急性毒性作用；而且经抗药性检测试验，菌剂中的巨大芽孢杆菌 ZL-1010株、枯草芽孢杆菌 ZL-1007株、球形芽孢杆菌 ZL-1008株和地衣芽孢杆菌 ZL-1013分别都对氟罗沙星、吡哌酸、头孢唑啉、青霉素等 38种常见抗生素敏感，易于用已知的常见抗生素进行控制。实施例 2 用于生态免冲水厕所的粪尿处理设备 The Shanghai Environmental Monitoring Center (China, Shanghai) was entrusted to conduct an environmental safety assessment of the bactericide A, and no obvious environmental safety problems were found. Specifically, according to the Chinese national standard GB7919-87 ("Cosmetic Safety Evaluation Procedures and Methods"), no acute toxicity was found by oral acute toxicity test in mice, and micronucleus test of mouse bone marrow polychromatic cells was found to have no Chromosomal aberrations, found to be non-irritating by animal skin irritation test; according to "Water and Wastewater Monitoring Analysis and Methods" (Fourth Edition, China's State Environmental Protection Administration, 2002), Ames test found no mutagenicity, algae The acute toxicity test found no obvious acute toxicity to algae, and the algae ecological test did not have obvious adverse effects on the algae ecology of natural water bodies; according to the Chinese national standard GB/T13267-91 ("water quality substances for freshwater fish (zebrafish) Acute toxicity test method), no obvious acute toxicity test by fish acute toxicity test; and Bacillus megaterium ZL-1010 strain, Bacillus subtilis ZL-1007 strain, spherical in the drug resistance test Bacillus sp. ZL-1008 and B. licheniformis ZL-1013 were respectively on fleroxacin and piperazine. 38 common antibiotics such as acid, cefazolin and penicillin are sensitive and easy to control with known common antibiotics. Example 2 Excrement treatment equipment for ecological flush-free toilet

如图 1-6所示，用于生态免冲水厕所的粪尿处理设备，包括暂时贮粪容器 1、刮动装置 2、发酵容器 3和机械传动装置 4，其特征在于，暂时贮粪容器 1上部带有开口 11，其可与蹲便器 5底部的活动挡板 51相对应；暂时贮粪容器 1与发酵容器 3连通，通过刮动装置 2 推进而将暂时贮粪容器 1内的粪尿推入发酵容器 3 ; 发酵容器 3内装有占约四分之三容积 (约 30kg)的实施例 1制备得到的微生物菌剂；发酵容器 3内有与机械传动装置 4联接的搅拌器 31 ; 而且发酵容器 3外底部设有加热装置 32。 · As shown in FIG. 1-6, the excrement treatment equipment for the ecological flush-free toilet includes a temporary storage container 1, a scraping device 2, a fermentation container 3, and a mechanical transmission device 4, which is characterized in that the temporary storage container 1 upper with an opening 11, which can be The movable baffle 51 at the bottom of the squatting pot 5 corresponds to each other; the temporary septic tank 1 is in communication with the fermentation container 3, and the excrement in the temporary septic container 1 is pushed into the fermentation container 3 by the squeezing device 2; There is a microbial agent prepared in Example 1 which occupies about three quarters of volume (about 30 kg); the fermentation vessel 3 has a stirrer 31 coupled to the mechanical transmission 4; and the outer bottom of the fermentation vessel 3 is provided with a heating device 32. . ·

在该粪尿处理设备中，机械传动装置 4联接带动发酵容器 3内的搅拌器 31进行搅拌。为了方便使用起见，该设备还包括电控装置，所述电控装置能控制刮动装置 2、机械传动装置 4和加热装置 32。所述电控装置可与免冲水厕所的蹲便室 100中的光电感应器 6相连接，所述光电感应器 6用于感应如厕者的活动。这样便可以根据光电感应器 6感应如厕者活动的情况，自动运行该粪尿处理设备。 In the excrement processing apparatus, the mechanical transmission unit 4 is coupled to agitator 31 in the fermentation vessel 3 for agitation. For convenience of use, the apparatus further includes an electronic control unit that can control the wiping unit 2, the mechanical transmission unit 4, and the heating unit 32. The electronic control unit can be coupled to a photosensor 6 in the toilet 100 of the flush-free toilet for sensing the activity of the toilet. In this way, the urinary treatment device can be automatically operated according to the situation in which the photoelectric sensor 6 senses the movement of the toilet.

在该粪尿处理设备中，所述刮动装置 2通过电动机 21带动来进行推进或拉出。其中，所述刮动装置 2通过齿轮 23传动来进行推进或拉出，或者所述刮动装置 2通过连杆 22传动来进行推进或拉出。实施例 3 生态免冲水厕所及其应用 In the excreta processing apparatus, the scraping device 2 is driven by the motor 21 to be pushed or pulled out. Here, the scraping device 2 is driven or pulled out by the gear 23, or the scraping device 2 is driven by the connecting rod 22 to advance or pull out. Example 3 Ecological flush-free toilet and its application

如图 1-6所示，生态免冲水厕所，包括设于基面 7上的蹲便室 100和设于基面 7下的处理室 200，其特征在于，蹲便室 100安装有蹲便器 5，所述蹲便器 5安装在基面 7上面；而且，处理室 200内安装有实施例 1中所述的粪尿处理设备，其中暂时贮粪容器 1上部带有开口 11，其与蹲便器 5底部的活动挡板 51相对应。 As shown in FIG. 1-6, the ecological flush-free toilet includes a squatting chamber 100 disposed on the base surface 7 and a processing chamber 200 disposed under the base surface 7, wherein the squatting chamber 100 is equipped with a squatting pan 5, the squatting pan 5 is mounted on the base surface 7; further, the excavation treatment device described in Embodiment 1 is installed in the processing chamber 200, wherein the temporary septic container 1 has an opening 11 at the upper portion thereof, and the squatting device The movable flap 51 at the bottom of the 5 corresponds.

在该生态免冲水厕所中，所述蹲便室 100中有光电感应器 6，所述光电感应器 6与所述粪尿处理设备中的电控装置相连接，而且其用于感应如厕者的活动。例如，当检测到有如厕者时，蹲便器 5底部的活动挡板 51打开，电控装置控制刮动装置 2呈复位状态；当检测到如厕者离开时，蹲便器 5底部的活动挡板 51关闭，电控装置控制刮动装置 2将粪尿推入发酵容器 3，然后复位，并可在适当时控制机械传动装置 4和加热装置 32运行。 In the ecological flush toilet, the squatting chamber 100 has a photoelectric sensor 6 connected to the electronic control device in the urinary treatment device, and used for sensing the toilet Activity. For example, when a toilet is detected, the movable flap 51 at the bottom of the squatting pan 5 is opened, the electric control device controls the scraping device 2 to be in a reset state; when the toilet seat is detected to be removed, the movable baffle at the bottom of the squatting pan 5 51 is closed, and the electronic control unit controls the scraping device 2 to push the excrement into the fermentation vessel 3, and then reset, and can control the mechanical transmission 4 and the heating device 32 to operate as appropriate.

另外在该生态免冲水厕所中，所述蹲便器 5底部的活动挡板 51的开合方式为水平移动式开合。此外其中，蹲便室（100 ) 还可以按需要安装有旋启窗、门、小便池、洗手池、排气管和 /或排气风扇，处理室（200)也可以安装有排气管和 /或排气风扇等设备。 In addition, in the ecological flush-free toilet, the movable flap 51 at the bottom of the squatting pan 5 is opened and closed in a horizontally movable manner. In addition, the toilet (100) can also be equipped with a swing window, a door, a urinal, a sink, an exhaust pipe and/or an exhaust fan as needed, and the processing chamber (200) can also be equipped with an exhaust pipe and / or equipment such as exhaust fans.

使用该生态免冲水厕所时，当人进入该生态免冲水厕所后，可以根据光电感应器 6的探测结果而自动开启蹲便器 5底部的活动挡板 51，以使如厕人使用；当人离开时，自动关闭蹲便器 5底部的活动挡板 51，并通过电控装置控制刮动装置 2将暂时贮粪容器 1内的粪尿推入装有 2公斤实施例 1制备的菌剂 A或菌剂 B的发酵容器 3，然后刮动装置 2复位。电控装置自动控制机械传动装置 4和加热装置 32的运行，根据微生物的性质、粪尿量、如厕频度、温度和湿度通过微电脑计算而调节机械传动装置 4和加热装置 32的运行，提供帮助微生物降解的条件。这样就不必在如厕频度高时长时间关闭搅拌器 31，因此进一步提高了微生物的降解效率，更进一步减少了产'生臭味的可能性，并提高了安全性和视觉感官接受程度。上述厕所在平均每天 50人次大便的情况下，连续 90天运行后，蹲便室 100 中的气味没有令人不愉快。实施例 4酿酒酵母 ZL-1011株 26S rRNA基因的鉴定 When the ecological flush-free toilet is used, when the person enters the ecological flush-free toilet, the movable baffle 51 at the bottom of the squatting pan 5 can be automatically opened according to the detection result of the photoelectric sensor 6, so that the toilet person can use it; When the person leaves, the movable baffle 51 at the bottom of the squatting device 5 is automatically closed, and the squirting device 2 is pushed by the electric control device to push the faeces and urine in the temporary septic container 1 into 2 kg of the bacterium A prepared in the first embodiment. Or the fermentation vessel 3 of the microbial agent B, and then the scraping device 2 is reset. The electronic control device automatically controls the operation of the mechanical transmission device 4 and the heating device 32, depending on the nature of the microorganism, the amount of feces, and The frequency, temperature and humidity of the toilet are adjusted by microcomputer calculations to regulate the operation of the mechanical transmission 4 and the heating device 32 to provide conditions that aid in microbial degradation. This eliminates the need to turn off the agitator 31 for a long time when the frequency of the toilet is high, thereby further improving the degradation efficiency of the microorganisms, further reducing the possibility of producing a odor, and improving the safety and visual sensory acceptance. The scent in the squatting chamber 100 was not unpleasant after the toilet was operated for 90 consecutive days with an average of 50 stools per day. Example 4 Identification of 26S rRNA Gene of Saccharomyces Cerevisiae ZL-1011 Strain

挑取生长至对数晚期的酿酒酵母 ZL-1011株的少量菌体置于已灭菌的 1.5ml离心管内，加入 lOOul裂解液（lOOmM Tris, 30mM EDTA, 0.5%SDS, pH8.0, 15P灭菌 30分钟备用）， 100°C水浴 15分钟，加入 100ull2.5M的醋酸钾后，在冰浴 30分钟， 4°C下离心（13000rpm， 5分钟），将上清液移入新的 1.5ml离心管中，加入等体积酚 -氯仿（24: 1， v/v) ，剧烈震荡后离心（13000rpm， 15分钟），将上清液移入新的 1.5ml离心管中。重复上一步操作，直到两相之间没有沉淀，将上清液移入另一已灭菌的 0.5ml离心管中。加入等体积冷异丙醇， -20 °C放置 15分钟后离心（13000rpm， 15分钟），用 100ul70%乙醇洗涤沉淀，用 lOOul无水乙醇洗涤沉淀，将沉淀放置于真空干燥器中抽干，加入 50ul已灭菌的超纯水， 4°C溶解 2小时，放置于 -20Ό备用。用于 26S rRNA基因 D1/D2区扩增的 PCR反应引物为 A small number of cells of Saccharomyces cerevisiae ZL-1011 strain grown to the late logarithm were picked and placed in a sterilized 1.5 ml centrifuge tube, and 100 ul of lysate (100 mM Tris, 30 mM EDTA, 0.5% SDS, pH 8.0, 15P) was added. The bacteria were allowed to stand for 30 minutes, and the water bath was kept at 100 ° C for 15 minutes. After adding 100 μl of 2.5 M potassium acetate, centrifuge in ice for 30 minutes, centrifuge at 4 ° C (13,000 rpm, 5 minutes), and transfer the supernatant to a new 1.5 ml centrifuge. In the tube, an equal volume of phenol-chloroform (24:1, v/v) was added, centrifuged vigorously (13,000 rpm, 15 minutes), and the supernatant was transferred to a new 1.5 ml centrifuge tube. Repeat the previous step until there is no precipitation between the two phases. Transfer the supernatant to another sterilized 0.5 ml centrifuge tube. An equal volume of cold isopropanol was added, placed at -20 ° C for 15 minutes, centrifuged (13,000 rpm, 15 minutes), the precipitate was washed with 100 ul of 70% ethanol, and the precipitate was washed with 100 ul of absolute ethanol, and the precipitate was placed in a vacuum desiccator and drained. Add 50 ul of sterilized ultrapure water, dissolve at 4 ° C for 2 hours, and place at -20 Torr for use. The PCR reaction primer for the amplification of the D1/D2 region of the 26S rRNA gene is

5'-GCATATCAATAAGCGGAGGAAAAG-3 ' (NL-1), 和 5'-GCATATCAATAAGCGGAGGAAAAG-3 ' (NL-1), and

5，-GGTCCGTGTTTCAAGACGG-3，（NL4)。 PCR反应体系（50 μΐ) 为: 50mmol/L KCl、 2 mmol/L MgCl₂、 10 mmol/L Tris-HCl(pH9.0) 引物 PI和引物 P2各 0.6μΜ 、样品 DNAl g、 Taq DNA酶 1U。 PCR反应条件为： 95°C 5 min, 94 °C 1 min, 52 °C 1 min, 72 °C 1 min20 秒， 36个循环； 72°C 8 min, 4°C保存。 PCR产物的测序采用 DNA自动测序仪（ABI PRISM 377 DNA Sequencer, ABI PRISM 3100 Genetic Analyzer)。测序得到的 26S rRNA基因的核苷酸序列如 Seq ID No.l所示。经对 Genbank数据库检索未发现相同序列，因此可用于表征本发明的菌剂 A。 pwky0041 * 5, -GGTCCGTGTTTCAAGACGG-3, (NL4). The PCR reaction system (50 μΐ) was: 50 mmol/L KCl, 2 mmol/L MgCl ₂ , 10 mmol/L Tris-HCl (pH 9.0) Primer PI and primer P2 0.6 μΜ each, sample DNA l g, Taq DNase 1 U . The PCR reaction conditions were: 95 ° C for 5 min, 94 ° C for 1 min, 52 ° C for 1 min, 72 ° C for 1 min and 20 seconds, 36 cycles; 72 ° C for 8 min, 4 ° C for storage. The PCR product was sequenced using an automated DNA sequencer (ABI PRISM 377 DNA Sequencer, ABI PRISM 3100 Genetic Analyzer). The nucleotide sequence of the 26S rRNA gene obtained by sequencing is shown as Seq ID No. 1. The same sequence was not found by searching the Genbank database and thus can be used to characterize the microbial agent A of the present invention. Pwky0041 *

PCT PCT

原件（提交） Original (submitted)

0-1 PCT/RO/134表（SAFE) 0-1 PCT/RO/134 (SAFE)

有关保藏的微生物或其他生物材 Microorganisms or other biological materials related to preservation

料的说明 (PCT细则第 13条之二） Description of the material (PCT Rule 13 bis)

0-1-1 软件版本 PCT-SAFE [EASY mode] 0-1-1 Software Version PCT-SAFE [EASY mode]

版本 3. 51 . 014. 189 MT/F0P 20060701 /0. 20. 5 Version 3. 51 . 014. 189 MT/F0P 20060701 /0. 20. 5

0-2 国际申请号 0-2 International Application Number

0-3 申请人或代理人的档案号 pwky0041 0-3 File number of the applicant or agent pwky0041

1 下面的说明与本申请说明书中此 1 The following instructions are related to this manual.

处提到的保藏的微生物或其他生 Deposited microorganisms or other raw

物材料相关： Material related:

1-1 页码 4 1-1 Page 4

1-2 行号： 6 1-2 line number: 6

1-3 保藏事项 1-3 Deposits

1-3-1 保藏单位名称 CGMCC 中国微生物菌种保藏管理委员会普通微生物中心 1-3-1 Name of the depository unit CGMCC China Microbial Culture Collection Management Committee General Microbiology Center

1-3-2 保藏单位地址中国微生物菌种保藏委员会，中国北京市 2714信箱，邮政编码： 100080， Be i j i ng (GN)。 1-3-2 Depository Address China Microbial Culture Collection Committee, 2714 Mailbox, Beijing, China, 100080, Be i j i ng (GN).

1-3-3 保藏曰期 2006年 5月 30日 (30. 05. 2006) 1-3-3 Preservation period May 30, 2006 (30. 05. 2006)

1-3-4 保藏号 CGMCC NO. 1724 1-3-4 Deposit No. CGMCC NO. 1724

1-5 本说明是对下列指定国所有指定国 ¹ 1-5 This note is designated States all designated States following ¹

2 下面的说明与本申请说明书中此 2 The following instructions are related to this manual.

物材料相关： Material related:

2-1 页码 4 2-1 Page 4

2-2 行号： 7 2-2 Line number: 7

2-3 保藏事项 2-3 preservation matters

2-3-1 保藏单位名称 CGMCC 中国微生物菌种保藏管理委员会普通微生物中心 2-3-1 Name of the depository unit CGMCC China Microbial Culture Collection Management Committee General Microbiology Center

2-3-2 保藏单位地址中国微生物菌种保藏委员会，中国北京市 2714信箱，邮政编码： 100080, Be i j i ng (GN)。 2-3-2 Depository Address China National Collection of Microorganisms, 2714 Mailbox, Beijing, China, 100080, Be i j i ng (GN).

2-3-3 保藏日期 2006年 5月 30日 (30. 05. 2006) 2-3-3 Date of Deposit May 30, 2006 (30. 05. 2006)

2-3-4 保藏号 GGMCC NO. 1725 2-3-4 Deposit No. GGMCC NO. 1725

2-5 本说明是对下列指定国所有指定国 2-5 This note is for all designated countries in the following designated countries.

pwky0041 Pwky0041

PCT PCT

原件（提交） Original (submitted)

3 下面的说明与本申请说明书中此 3 The following instructions are related to this manual.

物材料相关： Material related:

3-1 页码 4 3-1 Page 4

3-2 行号： 8 3-2 Line number: 8

3-3 保藏事项 3-3 Preservation

3-3-1 保藏单位名称 C6CC Centre General Chi no is de Cultures 3-3-1 Depository Name C6CC Centre General Chi no is de Cultures

Microbiol o iques Microbiol o iques

3-3-2 保藏单位地址 Chine - Com i te pour la col lection de cultures de micro-organismes, P.O. Box 2714, Beijing 100080 3-3-2 Depository Address Chine - Com i te pour la col lection de cultures de micro-organismes, P.O. Box 2714, Beijing 100080

3-3-3 保藏日期 2006年 5月 30日（30.05.2006〉 3-3-3 Deposit Date May 30, 2006 (30.05.2006)

3-3-4 保藏号 CGCCM NO.1726 3-3-4 Deposit No. CGCCM NO.1726

3-5 本说明是对下列指定国所有指定国 3-5 This note is for all designated countries in the following designated countries.

4 下面的说明与本申请说明书中此 4 The following instructions are related to this manual.

物材料相关： Material related:

4-1 页码 4 4-1 Page 4

4-2 行号： 9 4-2 Line number: 9

4-3 保藏事项 4-3 Preservation

4-3-1 保藏单位名称 CGMCC 中国微生物菌种保藏管理委员会普通微生物中心 4-3-1 Name of the depository unit CGMCC China Microbial Culture Collection Management Committee General Microbiology Center

4-3-2 保藏单位地址中国微生物菌种保藏委员会，中国北京巿 2714信箱，邮政编码： 100080, Beijing (GN)。 4-3-2 Depository Address China National Collection of Microorganisms, 2714, Beijing, China, Postcode: 100080, Beijing (GN).

4-3-3 保藏日期 2006年 5月 300 (30.05.2006) 4-3-3 Deposit Date May, 2006 300 (30.05.2006)

4-3-4 保藏号 CGMCC NO.1727 4-3-4 Deposit No. CGMCC NO.1727

4-5 本说明是对下列指定国所有指定国 4-5 This note is for all designated countries in the following designated countries.

5 下面的说明与本申请说明书中此 5 The following instructions are related to this manual.

物材料相关： Material related:

5-1 页码 4 5-1 Page 4

5-2 行号： 10 5-2 Line number: 10

5-3 保藏事项 5-3 Preservation

5-3-1 保藏单位名称 GGMCC 中国微生物菌种保藏管理委员会普通微生物中心 5-3-1 Name of the depository unit GGMCC China Microbial Culture Collection Management Committee General Microbiology Center

5-3-2 保藏单位地址中国微生物菌种保藏委员会，中国北京市 2714信箱，邮政编码： 100080， Beijing (GN)。 5-3-2 Depository Address China National Collection of Microorganisms, 2714 Mailbox, Beijing, China, 100080, Beijing (GN).

5-3-3 保藏日期 2007年 4月 25日（25.04.2007) 5-3-3 Deposit Date April 25, 2007 (25.04.2007)

5-3-4 保藏号 CGMCC NO.2020 5-3-4 Deposit No. CGMCC NO.2020

5-5 本说明是对下列指定国所有指定国原件（提交） 5-5 This note is for all designated countries in the following designated countries. Original (submitted)

下面的说明与本申请说明书中此 The following description and this description of the application

物材料相关： Material related:

-1 页码 4 -1 Page 4

-2 行号： 1 1 -2 Line number: 1 1

-3 保藏事项 -3 Preservation

-3-1 保藏单位名称 CGMCC 中国微生物菌种保藏管理委员会普通微生物中心 -3-2 保藏单位地址中国微生物菌种保藏委员会，中国北京市 2714信箱，邮政编码： 100080, Be i j i ng (GN)。 -3-1 Name of the depository unit CGMCC Ordinary Microbiology Center of China Microbial Culture Collection Management Committee -3-2 Depository Address China National Collection of Microorganisms, 2714, Beijing, China, Post Code: 100080, Be i j i ng (GN).

-3-3 保藏曰期 2007年 4月 25日 (25. 04. 2007) -3-3 Preservation Period April 25, 2007 (25. 04. 2007)

-3-4 保藏号 CGMCC NO. 2018 -3-4 Deposit No. CGMCC NO. 2018

-5 本说明是对下列指定国所有指定国 -5 This note is for all designated countries in the following designated countries.

物材科相关： Material Section related:

-1 页码 4 -1 Page 4

-2 行号： 12 -2 Line number: 12

-3 保藏事项 -3 Preservation

-3-1 保藏单位名称 CGMCC 中国微生物菌种保藏管理委员会普通微生物中心 -3-2 保藏单位地址中国微生物菌种保藏委员会，中国北京市 2714信箱，邮政编码： 100080, Be i j i ng (CN；)。 -3-1 Name of Depository Organization CGMCC General Microbiological Center of China Microbial Culture Collection Management Committee-3-2 Depository Address China National Collection of Microorganisms, 2714 Mailbox, Beijing, China Post Code: 100080, Be iji ng (CN;) .

-3-3 保藏日期 2007年 4月 25日 (25. 04. 2007) -3-3 Date of Deposit April 25, 2007 (25. 04. 2007)

-3-4 保藏号 CGMCC NO. 2021 -3-4 Deposit No. CGMCC NO. 2021

-5 本说明是对下列指定国所有指定国由受理局填写 -5 This note is for all designated countries in the following designated countries.

-5 国际局收到本表格日期： -5 The International Bureau received the date of this form:

-5-1 受权官员 -5-1 Authorized Officer

Claims

权利要求书 Claim

1、一种用于生态免冲水厕所的粪尿处理设备，包括暂时贮粪容器（1)、刮动装置（2)、发酵容器（3)和机械传动装置（4)，其特征在于，暂时贮粪容器（1)上部带有开口（11)，其可与蹲便器（5) 底部的活动挡板（51)相对应；暂时贮粪容器（1) 与发酵容器（3) 连通，通过刮动装置（2)推进而将暂时贮粪容器（1) 内的粪尿推入发酵容器（3); 发酵容器（3)内装有微生物菌剂；发酵容器（3)内有与机械传动装置（4)联接的搅拌器（31); 而且发酵容器（3) 外底部设有加热装置（32)。 1. A waste urine treatment device for an ecological flush-free toilet, comprising a temporary storage container (1), a scraping device (2), a fermentation container (3) and a mechanical transmission device (4), characterized in that The temporary storage container (1) has an opening (11) at the upper portion corresponding to the movable baffle (51) at the bottom of the squatting device (5); the temporary septic container (1) is connected to the fermentation container (3), The scraping device (2) advances to push the excrement in the temporary storage container (1) into the fermentation container (3); the fermentation container (3) contains the microbial agent; the fermentation container (3) has the mechanical transmission device (4) The coupled agitator (31); and the outer bottom of the fermentation vessel (3) is provided with a heating device (32).

2、根据权利要求 1 所述的粪尿处理设备，其特征在于，该设备还包括电控装置，所述电控装置能控制刮动装置（2)、机械传动装置（4)和加热装置（32)。 2. The excreta processing apparatus according to claim 1, characterized in that the apparatus further comprises an electric control device capable of controlling the wiping device (2), the mechanical transmission device (4) and the heating device ( 32).

3、根据权利要求 2所述的粪尿处理设备，其特征在于，所述电控装置可与免冲水厕所的蹲便室（100) 中的光电感应器（6) 相连接，所述光电感应器（6)用于感应如厕者的活动。 3. The excreta processing apparatus according to claim 2, wherein the electronic control unit is connectable to a photosensor (6) in a squatting chamber (100) of the flush-free toilet, the photoelectric The sensor (6) is used to sense the activity of the toilet.

4、根据权利要求 1或 2所述的粪尿处理设备，其特征在于，所述微生物菌剂含有芽抱杆菌，而且含有酿酒酵母和 /或荧光假单胞菌。 The excrement processing apparatus according to claim 1 or 2, wherein the microbial agent contains Bacillus licheniformis and contains Saccharomyces cerevisiae and/or Pseudomonas fluorescens.

5、根据权利要求 4所述的粪尿处理设备，其特征在于，所述芽孢杆菌优选是选自保藏号为 CGMCC No.1724的巨大芽孢杆菌 (BacUlus megaterium) 株、保藏号为 CGMCCThe excreta processing apparatus according to claim 4, wherein the Bacillus is preferably selected from the group consisting of BacUlus megaterium having a deposit number of CGMCC No. 1724, and the deposit number is CGMCC.

No.1725的枯草芽孢杆菌 Bacillus subtilis) ZL-1007株、保藏号为 CGMCC No.1726的球形芽孢杆菌 Bacillus sphaericus) ZL-1008株、保藏号为 CGMCC No.1727的环状芽抱杆菌 Bactilus circidan^ ZL-1009株、保藏号为 CGMCC No.2020的地衣芽孢杆菌 BaciUus licheniformis) ZL-1013株中的一种或多种，所述酿酒酵母优选是保藏号为 CGMCC No.2018 的酉良酒酵母 accharomyces cerevisiae) ZL-1011 株，和 /或所述荧光假单胞菌优选是保藏号为 CGMCC No.2021的荧光假单胞菌 (Pse domonas fluorescens) ZL-1014株。 No.1725 Bacillus subtilis ZL-1007 strain, Bacillus sphaericus ZL-1008 with CGMCC No.1726, Bactilus circidan^ with CGMCC No.1727 One or more strains of ZL-1009 strain, Bacillus licheniformis), which is CGMCC No. 2020, preferably Saccharomyces cerevisiae accumomyces with the accession number CGMCC No. 2018 The cerevisiae) ZL-1011 strain, and/or the Pseudomonas fluorescens is preferably the Pse domonas fluorescens ZL-1014 strain deposited under the number CGMCC No. 2021.

6、根据权利要求 1所述的粪尿处理设备，其特征在于，所述刮动装置（2)通过齿轮（23) 传动来进行推进或拉出。 6. The excreta processing apparatus according to claim 1, characterized in that the scraping means (2) is driven or pulled out by a gear (23).

7、根据权利要求 1所述的粪尿处理设备，其特征在于，所述刮动装置（2)通过连杆 (22) 传动来进行推进或拉出。 The excreta processing apparatus according to claim 1, characterized in that the scraping means (2) is driven or pulled out by a link (22).

8、一种生态免冲水厕所，包括设于基面（7)上的蹲便室（100) 和设于基面（7)下的处理室（200)，其特征在于，蹲便室（100) 中安装有蹲便器（5)，所述蹲便器（5) 安装在基面（7)上面；而且，处理室（200) 内安装有权利要求 1至 7之任一项所述的粪尿处理设备，其中暂时贮粪容器（1)上部带有开口（11)，其与蹲便器（5)底部的活动挡板（51) 相对应。 8. An ecological flush-free toilet comprising a squatting chamber (100) disposed on a base surface (7) and a processing chamber (200) disposed under the base surface (7), characterized in that: a squatting chamber ( 100) is installed with a squatting pan (5), the squatting pan (5) is mounted on the base surface (7); and the treatment chamber (200) is equipped with the dung according to any one of claims 1 to 7. a urine treatment device, wherein the temporary storage container (1) has an opening (11) at the upper portion thereof, and a movable baffle (51) at the bottom of the squatting pan (5) Corresponding.

9、根据权利要求 8所述的生态免冲水厕所，其特征在于，所述蹲便室（100) 中有光电感应器（6)，所述光电感应器（6) 与所述粪尿处理设备中的电控装置相连接，而且其用于感应如厕者的活动。 The ecological flush-free toilet according to claim 8, wherein the squatting chamber (100) has a photoelectric sensor (6), and the photoelectric sensor (6) and the urinary treatment The electronic controls in the device are connected and are used to sense the activities of the toilet.

10、根据权利要求 8或 9所述的生态免冲水厕所，其特征在于，所述蹲便器（5 )底部的活动挡板（51 ) 的开合方式为水平移动式开合。 The ecological flush-free toilet according to claim 8 or 9, wherein the movable baffle (51) at the bottom of the squatting pan (5) is opened and closed horizontally.