WO2008034337A1 - Appareil de traitement d'excréments et d'urines conçu pour toilettes écologiques sans chasse - Google Patents

Appareil de traitement d'excréments et d'urines conçu pour toilettes écologiques sans chasse Download PDF

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Publication number
WO2008034337A1
WO2008034337A1 PCT/CN2007/002591 CN2007002591W WO2008034337A1 WO 2008034337 A1 WO2008034337 A1 WO 2008034337A1 CN 2007002591 W CN2007002591 W CN 2007002591W WO 2008034337 A1 WO2008034337 A1 WO 2008034337A1
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WO
WIPO (PCT)
Prior art keywords
toilet
cgmcc
squatting
strain
excrement
Prior art date
Application number
PCT/CN2007/002591
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English (en)
Chinese (zh)
Inventor
Longji Wei
Ling Zhang
Original Assignee
Longji Wei
Ling Zhang
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from CNB2006101128506A external-priority patent/CN100540655C/zh
Priority claimed from CN2007101428134A external-priority patent/CN101139555B/zh
Application filed by Longji Wei, Ling Zhang filed Critical Longji Wei
Publication of WO2008034337A1 publication Critical patent/WO2008034337A1/fr

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Classifications

    • A—HUMAN NECESSITIES
    • A47—FURNITURE; DOMESTIC ARTICLES OR APPLIANCES; COFFEE MILLS; SPICE MILLS; SUCTION CLEANERS IN GENERAL
    • A47K—SANITARY EQUIPMENT NOT OTHERWISE PROVIDED FOR; TOILET ACCESSORIES
    • A47K11/00—Closets without flushing; Urinals without flushing; Chamber pots; Chairs with toilet conveniences or specially adapted for use with toilets
    • A47K11/02—Dry closets, e.g. incinerator closets
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/02—Aerobic processes
    • C02F3/12—Activated sludge processes
    • C02F3/1236—Particular type of activated sludge installations
    • C02F3/1242—Small compact installations for use in homes, apartment blocks, hotels or the like
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W10/00—Technologies for wastewater treatment
    • Y02W10/10—Biological treatment of water, waste water, or sewage

Definitions

  • the present invention belongs to the field of sanitary equipment, and relates to an excreta treatment apparatus for an ecological flush-free toilet and an ecological flush-free toilet including the same and a microbial agent used therein.
  • BACKGROUND OF THE INVENTION - Existing flush-free toilets come in two main forms: packaged flush-free toilets and ecological flush-free toilets. Packed flush-free toilets require packaging materials (such as plastic bags), which not only have higher cost of use, cleaning and transportation costs, but also cause secondary pollution due to the difficult degradation of plastic products.
  • packaging materials such as plastic bags
  • the medium dung bag is easy to freeze, and the low temperature adaptability is poor, which limits the applicable area.
  • the ecological flush-free toilet is a resource-based, harmless and reduced treatment of human excrement by the biodegradation of aerobic microorganisms, which overcomes the defects of the packaged flush-free toilet.
  • the Chinese patent CN1189253C and the Chinese patent or patent application CN1773034A, CN2761126Y, etc. disclose ecological flush toilets, except for being placed in fixed places. It can also be widely applied to mobile sites such as trains and automobiles (for example, see Chinese patents CN2803845Y, CN2801575Y, CN2838507Y).
  • these existing ecological flush-free toilets are also used in the prior art.
  • the fermentation vessel in the bioreactor, the main engine, etc. is directly connected to the bottom of the squatting pot.
  • the toilet person can easily see and directly contact the feces and urine degradants mixed with the microbial agent inside the fermentation vessel, and the stirrer. Internal equipment. Although the odor has been greatly improved under the action of the microbial agent, it also brings an uncomfortable feeling to the visual sense of the toilet person.
  • the movable baffle is opened for safety. For example, it is generally necessary to turn off the agitator.
  • a new excreta treatment device for ecological flush-free toilets which adds a temporary septic container that can temporarily store fresh urine when going to the toilet and a scratch with no sharp edges that are accessible.
  • the device makes the ecological flush-free toilet including the equipment not only retains the advantages of the existing ecological flush-free toilet, such as low odor, good low temperature adaptability, compressed manure and urine volume, and is easy to clear and transport, and overcomes the advantages.
  • the disadvantages of poor visual perception, high degradation efficiency, and danger are likely to occur, and the prospect of popularization and application is further enhanced.
  • the object of the present invention is to provide an excrement treatment device for an ecological flush-free toilet, which adds a temporary storage container capable of temporarily storing excrement and urine and has no accessibility on the basis of the existing ecological flush-free toilet.
  • the present invention provides an excreta processing apparatus for an ecological flush-free toilet, comprising a temporary storage container (1), a scraping device (2), and a fermentation container (3) And a mechanical transmission device (4), characterized in that the upper portion of the temporary storage container (1) has an opening (11) corresponding to the movable baffle (51) at the bottom of the squatting device (5); 1) communicating with the fermentation vessel (3), pushing the excrement in the temporary storage container (1) into the fermentation vessel (3) by pushing the scraping device (2); the fermentation vessel (3) is filled with the microbial agent
  • the fermentation vessel (3) has a stirrer (31) coupled to the mechanical transmission (4); and the outer bottom of the fermentation vessel (3) is provided with a heating device (32).
  • the mechanical transmission (4) is coupled to agitator (31) in the fermentation vessel (3) for agitation.
  • the device may further include an electronic control device capable of controlling the scraping device
  • the electronic control unit can be automatically controlled, such as by a programmable microprocessor chip.
  • the electronic control unit is also connectable to a photoconductor (6) in the toilet (100) of the flush-free toilet, the photoelectric sensor (6) being used to sense the activity of the toilet.
  • the urinary treatment device can be automatically operated according to the condition in which the photoconductor (6) senses the movement of the occupant.
  • the electronic control device can also control the mechanical transmission device by means of the micro-computer device according to the nature of the microorganism and/or the amount of fecal urine, toilet frequency, temperature and/or humidity sensed by the connected sensor (4) Thereby driving the agitator (31) to operate and controlling the operation of the heating device (32), increasing the degree of looseness, increasing the ventilation, controlling the moisture of the excrement in the fermentation vessel, and maintaining the optimal degradation activity of the microbial species, temperature, humidity and oxygenation. The amount, so that the depletion of the excrement can be achieved in situ. Due to the provision of temporary storage capacity The (1), therefore, the agitator (31) controlled by the electronic control device can be forced to stop running when the movable baffle (51) is opened, so that the process of degradation of the microbial strain is smoother.
  • the mechanical transmission (4), the agitator (31) and the heating device (32) may respectively be corresponding mechanical transmissions, agitators and the like described in Chinese Patent or Patent Application No. CN1773034A or CN2761126Y. heating equipment.
  • the agitator is a single stir cage, a double stir cage or a multi-stirred cage structure
  • the stirrer shaft is coupled with a stirrer
  • the stirrer is spiral
  • the stirrer shaft and the stirrer shaft are Coupling
  • both ends of the stirring cage shaft are arranged on the front and rear walls of the fermentation vessel
  • one end of the stirring cage shaft is provided with a through-hole sliding bearing
  • the other end is equipped with a blind hole sliding bearing
  • the heating device comprises a heating protective cover and a heating element, heating The protective cover is coupled to the outer side wall of the bottom of the fermentation vessel, and the heating cover is provided with heating elements, the heating elements are arranged in a roundabout manner and controlled by the electronic control device
  • the mechanical transmission device may comprise a motor base, a single row of sprocket wheels, a chain, Double row sprocket, reducer, motor, and adjustment bolts.
  • a reducer and a motor are mounted on the upper motor base through adjusting bolts.
  • the output shaft of the reducer is equipped with a double-row sprocket, the double-row sprocket is linked with two chains, and the two chains respectively link two single-row sprocket, two single-row sprocket Installed on the two stirrer shafts of the two agitators.
  • the fermentation vessel (3) may be provided with a ventilating device, such as an exhaust pipe and/or an exhaust fan, to ventilate the interior of the fermentation vessel (3) and maintain humidity.
  • a ventilating device such as an exhaust pipe and/or an exhaust fan
  • the ventilating device is also controlled by the aforementioned electronic control device.
  • the fermentation vessel (3) may have a door that can be opened and closed, so that the microbial-treated excrement in the fermentation vessel (3) can be cleaned up when necessary, and a new microbial agent can be added.
  • the temporary storage tank (1) may also have a door that can be opened and closed, and it is convenient to clean the dirt left in the corner of the temporary storage container (1) when necessary. This will be apparent to those skilled in the art.
  • the squeezing device (2) can preferably be propelled or pulled (pulled out, also referred to herein as a reset) by the motor (21), wherein various transmissions can be used.
  • the scraping device (2) is driven or pulled out by gearing (23).
  • gearing This is illustrated in detail in Figures 1 and 4, in which the motor (21) drives the gear unit (23) to drive or pull the scraping device (2).
  • the scraping device (2) can also be driven or pulled out by a connecting rod (22). Specific examples are shown in Figures 5 and 6, which are in reset and boost states, respectively.
  • the portion of the scraping device (2) in the temporary storage container (1) has no sharp edges that are accessible.
  • the microbial agent contained in the excrement treatment device contains Bacillus and contains Saccharomyces cerevisiae and/or fluorescent fake Monocytogenes. More preferably, the microbial agent consists essentially of one or more strains of Bacillus and one or more strains selected from the group consisting of Saccharomyces cerevisiae and Pseudomonas fluorescens.
  • Bacillus includes, but is not limited to, Bacillus megaterium, Bacillus subtilis, Bacillus sphaericus, Bacillus circulans, and Bacillus licheniformis.
  • the term "consisting essentially of” as used herein means that the bacteria listed in the term account for the vast majority of the total amount of bacteria contained in the microbial agent, usually accounting for more than 90%, preferably The selection does not substantially add other bacteria.
  • the strain used in the microbial agent used in the present invention is far less than the microbial agent disclosed in Chinese Patent No. CN1189253C, but it can still achieve good deodorization and reduction of excrement in the use of the excrement treatment device of the first aspect of the present invention. Effect.
  • the weight ratio between each of the above two strains contained in the microbial agent is preferably not more than 1: 0.1-10, that is, the amount of the most abundant fungus in the microbial agent is not more than It is 10 times, and most preferably substantially equal, of the strain having the smallest content.
  • the Bacillus is preferably selected from the group consisting of Bacillus megaterium ZL-1010 strain deposited under the accession number CGMCC No. 1724, and Bacillus subtilis ZL-1007 deposited under the accession number CGMCC No. 1725. Strain Bacillus sp. sphaericus ZL-1008 with CGMCC No. 1726, Bacillus circumatum 340 with strain CGMCC No. 1727, and CGMCC No. 2020 One or more strains of Bacillus licheniformis) ZL-1013, preferably Saccharomyces cerevisiae ZL-1011 strain deposited under the accession number CGMCC No.
  • the bacterium is preferably the Pseudomonas fluorescens iPseudomonas fluoresc s ZL-1014 strain deposited under the number CGMCC No. 2021.
  • the above strains were deposited in the General Microbiology Center of the China Microbial Culture Collection Management Committee (Beijing, China), and the dates of ZL-1010, ZL-1007, ZL-1008 and ZL-1009 were all deposited on May 30, 2006. The date of storage of ZL-1013 strain, ZL-1011 strain and ZL-1014 strain was April 25, 2007.
  • a specific microbial formulation includes Bacillus megaterium ZL-1010 strain, Bacillus subtilis ZL-1007 strain, Bacillus sphaericus ZL-1008 strain, Bacillus licheniformis ZL-1013 and Saccharomyces cerevisiae ZL-1011 strain.
  • a specific bacterial formulation comprises Bacillus subtilis ZL-1007 strain, Bacillus licheniformis ZL-1013 strain, and Pseudomonas fluorescens ZL-1014 strain.
  • the above-mentioned fungicides were commissioned by the Shanghai Environmental Monitoring Center (China, Shanghai) for environmental safety evaluation. No obvious environmental safety problems were found, so it was safe to promote the application, and the ingredients were clear and easy to control quality.
  • Saccharomyces cerevisiae ZL-1011 strain has flocculation, and our study also clarified the molecular classification background of S. cerevisiae ZL-1011 strain, which has the following 26S rRNA gene sequence different from the existing known strains (Seq ID No.
  • TTCATCTTTC CATCACTGTA CTTGTTCGCT ATCGGTCTCT CGCCAATATT TAGCTTTAGA TGGAATTTAC CACCCACTTA GAGCTGCATT CCCAAACAAC TCGACTCTTC GAAGGCACTT TACAAAGAAC ' CGCACTCCTC GCCACACGGG ATTCTCACCC TCTATGACGT CCTGTTCCAA GGAACATAGA CAAGGAACGG CCCCAAAGTT GCCCTCTCCA AATTACAACT CGGGCACCGA AGGTACCAGA TTTCAAATTT GAGCTTTTGC CGCTTCACTC GCCGTTACTA
  • the microbial agent usually further comprises a suitable filler, for example, it may contain one or more of wood chips, sawdust, shavings, bagasse, crop straw or shell. At least 90% of the filler particles are pulverized to a size of not more than 0.5 cm * 0.5 cm, preferably at least 90% pulverized to a size of not more than 0.1 cm * 0.1 cm.
  • the filler is mixed with the excrement to increase the porosity to promote ventilation, and can be shaped into a microbial agent for easy addition to the fermentation vessel (3) of the device.
  • the amount of filler in the microbial agent usually accounts for the majority, usually accounting for more than 95% of the total, such as 98%, 99% or 99.9%.
  • the volume of the added microbial agent does not overflow into the temporary storage container (1), usually from one-third to five-fifth of the fermentation container (3), and preferably the volume of the microbial agent accounts for the fermentation container (3). Three-quarters of the volume.
  • the amount of the above-mentioned microbial agent can be determined according to the total amount of daily feces and urine (which can also be expressed as the number of toilets) of the toilet person to be treated, and the microbial agent of the present invention and the average daily toilet
  • the relationship between the number of people can usually reach 100 person/30 kg of bacteria, preferably less than 80 times / 30 kg of bacteria, as in the embodiment of the invention is about 50 times / 30 kg of bacteria, can be used continuously for 3 Months or longer without the need to change the bacteria.
  • the present invention can provide the above microbial agents.
  • the excreta treating apparatus comprising the microbial agent of the first aspect of the invention is preferably used for treating feces.
  • the present invention also provides an ecological flush-free toilet comprising a squatting chamber (100) disposed on a base surface (7) and a processing chamber (200) disposed under the base surface (7).
  • the utility model is characterized in that the squatting chamber (100) is equipped with a squatting pan (5), the squatting pan (5) is mounted on the base surface (7); and the treatment chamber (200) is installed with the excrement treatment described above.
  • the device, wherein the temporary storage container (1) has an opening (11) at the upper portion corresponding to the movable baffle (51) at the bottom of the squatting pan (5).
  • the squatting chamber (100) has a photoelectric sensor (6), and the photoelectric sensor (6) is connected to the electronic control device in the urinary processing device. And it is used to sense the activities of the toilet.
  • the movable baffle (51) at the bottom of the squatting pan (5) is opened, the electric control device controls the striking device (2) to be in a reset state; when the toilet occupant is detected, the squat pan (5)
  • the bottom flap (51) is closed, the electric control device controls the scraper (2) to push the excrement into the fermentation vessel (3), then reset, and control the machine when appropriate
  • the transmission (4) and the heating device (32) operate.
  • the opening and closing manner of the movable baffle (51) at the bottom of the squatting pan (5) is preferably a horizontally movable opening and closing.
  • the toilet (100) can also be equipped with a swing window, a door, a urinal, a sink, an exhaust pipe and/or an exhaust fan.
  • the processing chamber (200) can also be equipped with an exhaust pipe and/or Or equipment such as an exhaust fan, these will be apparent to those of ordinary skill in the art.
  • the temporary storage container (1) can be made of fiberglass, stainless steel, etc.
  • the photoelectric sensor (6) has a commercially available infrared sensor.
  • the present invention will be described in detail below through the specific drawings and embodiments. It is to be understood that the specific examples and drawings are not to be construed as limiting. It is apparent that those skilled in the art can make various modifications and changes to the present invention within the scope of the present invention, and such modifications and changes are also included in the scope of the present invention.
  • the present invention is hereby incorporated by reference in its entirety in its entirety in the entireties in the the the the the the the the the the the the the Repeat the same.
  • FIG. 1 is a schematic view of a waste water treatment apparatus for a flush-free toilet according to the present invention, which uses a gear transmission.
  • 2 is a detailed schematic view showing a part of the structure of the flush-free toilet of the present invention.
  • Figure 3 is a schematic view showing the overall structure of the flush-free toilet of the present invention.
  • Figure 4 is an enlarged view of the geared joint pointed by F in Figure 1.
  • Fig. 5 is a schematic view of the excrement treatment apparatus for a flush-free toilet according to the present invention, which uses a link transmission device in a reset state.
  • Fig. 6 is a schematic view of the excrement processing apparatus for a flush-free toilet according to the present invention, which uses a link transmission device in a pushed-out state. detailed description
  • the six strains were separately cultured in liquid.
  • the medium used was: peptone 10.0 g, yeast extract 1.0 g, molasses 5.0 g, NaC1 5 g, water 1.0 liter, pH adjusted to 7.0-7.2 with 1 M NaOH, 115 degree sterilization 30 minutes.
  • the culture temperature and culture time of each strain were slightly different, and the Pseudomonas fluorescens CGMCC No. 2021 strain was shake cultured for 30-30 hours on a 30-32 ⁇ shaker (200 rpm), while the other 5 Bacillus strains were cultured. The mixture was shaken at 35-37 ° C for shaking (200 rpm) for 36-40 hours.
  • 1 kg of the sterilized wood chips and the fermentation broth were separately mixed and dried at room temperature to prepare a microbial agent containing only a single strain.
  • Bacterial agent A Bacillus megaterium ZL-1010 strain, Bacillus subtilis ZL-1007 strain, Bacillus sphaericus ZL-1008 strain, Bacillus licheniformis ZL-1013 and Saccharomyces cerevisiae ZL-1011 strain.
  • Bacterial agent B Bacillus subtilis ZL-1007 strain, Bacillus licheniformis ZL-1013 strain and Pseudomonas fluorescens ZL-1014 strain.
  • the excrement treatment equipment for the ecological flush-free toilet includes a temporary storage container 1, a scraping device 2, a fermentation container 3, and a mechanical transmission device 4, which is characterized in that the temporary storage container 1 upper with an opening 11, which can be The movable baffle 51 at the bottom of the squatting pot 5 corresponds to each other; the temporary septic tank 1 is in communication with the fermentation container 3, and the excrement in the temporary septic container 1 is pushed into the fermentation container 3 by the squeezing device 2; There is a microbial agent prepared in Example 1 which occupies about three quarters of volume (about 30 kg); the fermentation vessel 3 has a stirrer 31 coupled to the mechanical transmission 4; and the outer bottom of the fermentation vessel 3 is provided with a heating device 32. . ⁇
  • the mechanical transmission unit 4 is coupled to agitator 31 in the fermentation vessel 3 for agitation.
  • the apparatus further includes an electronic control unit that can control the wiping unit 2, the mechanical transmission unit 4, and the heating unit 32.
  • the electronic control unit can be coupled to a photosensor 6 in the toilet 100 of the flush-free toilet for sensing the activity of the toilet. In this way, the urinary treatment device can be automatically operated according to the situation in which the photoelectric sensor 6 senses the movement of the toilet.
  • the scraping device 2 is driven by the motor 21 to be pushed or pulled out.
  • the scraping device 2 is driven or pulled out by the gear 23, or the scraping device 2 is driven by the connecting rod 22 to advance or pull out.
  • Example 3 Ecological flush-free toilet and its application
  • the ecological flush-free toilet includes a squatting chamber 100 disposed on the base surface 7 and a processing chamber 200 disposed under the base surface 7, wherein the squatting chamber 100 is equipped with a squatting pan 5, the squatting pan 5 is mounted on the base surface 7; further, the excavation treatment device described in Embodiment 1 is installed in the processing chamber 200, wherein the temporary septic container 1 has an opening 11 at the upper portion thereof, and the squatting device The movable flap 51 at the bottom of the 5 corresponds.
  • the squatting chamber 100 has a photoelectric sensor 6 connected to the electronic control device in the urinary treatment device, and used for sensing the toilet Activity. For example, when a toilet is detected, the movable flap 51 at the bottom of the squatting pan 5 is opened, the electric control device controls the scraping device 2 to be in a reset state; when the toilet seat is detected to be removed, the movable baffle at the bottom of the squatting pan 5 51 is closed, and the electronic control unit controls the scraping device 2 to push the excrement into the fermentation vessel 3, and then reset, and can control the mechanical transmission 4 and the heating device 32 to operate as appropriate.
  • the movable flap 51 at the bottom of the squatting pan 5 is opened and closed in a horizontally movable manner.
  • the toilet (100) can also be equipped with a swing window, a door, a urinal, a sink, an exhaust pipe and/or an exhaust fan as needed, and the processing chamber (200) can also be equipped with an exhaust pipe and / or equipment such as exhaust fans.
  • the movable baffle 51 at the bottom of the squatting pan 5 can be automatically opened according to the detection result of the photoelectric sensor 6, so that the toilet person can use it;
  • the movable baffle 51 at the bottom of the squatting device 5 is automatically closed, and the squirting device 2 is pushed by the electric control device to push the faeces and urine in the temporary septic container 1 into 2 kg of the bacterium A prepared in the first embodiment. Or the fermentation vessel 3 of the microbial agent B, and then the scraping device 2 is reset.
  • the electronic control device automatically controls the operation of the mechanical transmission device 4 and the heating device 32, depending on the nature of the microorganism, the amount of feces, and
  • the frequency, temperature and humidity of the toilet are adjusted by microcomputer calculations to regulate the operation of the mechanical transmission 4 and the heating device 32 to provide conditions that aid in microbial degradation. This eliminates the need to turn off the agitator 31 for a long time when the frequency of the toilet is high, thereby further improving the degradation efficiency of the microorganisms, further reducing the possibility of producing a odor, and improving the safety and visual sensory acceptance.
  • the scent in the squatting chamber 100 was not unpleasant after the toilet was operated for 90 consecutive days with an average of 50 stools per day.
  • a small number of cells of Saccharomyces cerevisiae ZL-1011 strain grown to the late logarithm were picked and placed in a sterilized 1.5 ml centrifuge tube, and 100 ul of lysate (100 mM Tris, 30 mM EDTA, 0.5% SDS, pH 8.0, 15P) was added. The bacteria were allowed to stand for 30 minutes, and the water bath was kept at 100 ° C for 15 minutes. After adding 100 ⁇ l of 2.5 M potassium acetate, centrifuge in ice for 30 minutes, centrifuge at 4 ° C (13,000 rpm, 5 minutes), and transfer the supernatant to a new 1.5 ml centrifuge.
  • the PCR reaction system (50 ⁇ ) was: 50 mmol/L KCl, 2 mmol/L MgCl 2 , 10 mmol/L Tris-HCl (pH 9.0) Primer PI and primer P2 0.6 ⁇ each, sample DNA l g, Taq DNase 1 U .
  • the PCR reaction conditions were: 95 ° C for 5 min, 94 ° C for 1 min, 52 ° C for 1 min, 72 ° C for 1 min and 20 seconds, 36 cycles; 72 ° C for 8 min, 4 ° C for storage.
  • the PCR product was sequenced using an automated DNA sequencer (ABI PRISM 377 DNA Sequencer, ABI PRISM 3100 Genetic Analyzer).
  • the nucleotide sequence of the 26S rRNA gene obtained by sequencing is shown as Seq ID No. 1. The same sequence was not found by searching the Genbank database and thus can be used to characterize the microbial agent A of the present invention.
  • Microorganisms or other biological materials related to preservation are Microorganisms or other biological materials related to preservation

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  • Life Sciences & Earth Sciences (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Microbiology (AREA)
  • Hydrology & Water Resources (AREA)
  • Engineering & Computer Science (AREA)
  • Environmental & Geological Engineering (AREA)
  • Water Supply & Treatment (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Treatment Of Sludge (AREA)

Abstract

L'invention concerne des toilettes écologiques sans chasse et un appareil de traitement d'excréments et d'urines, ainsi qu'un agent microbien conçus pour des toilettes écologiques sans chasse. Ledit appareil de traitement d'excréments et d'urines comporte un boîtier de stockage temporaire (1), un moyen de raclage (2), un boîtier de ferments (3), et un train d'engrenages (4). Le boîtier de stockage temporaire (1) présente une ouverture (11) sur sa partie supérieure correspondant au fond d'une cuvette de toilette traditionnelle (5), et communique avec le boîtier de ferments (3). Les excréments et les urines du boîtier de stockage temporaire (1) sont poussés dans le boîtier de ferments (3) par le moyen de raclage (2). L'agent microbien est retenu dans le boîtier de ferments (3). Un mélangeur (31) est couplé au train d'engrenages (4) à l'intérieur du boîtier de ferments (3). Un moyen de chauffage (32) est placé sur le fond extérieur du boîtier de ferments (3).
PCT/CN2007/002591 2006-09-05 2007-08-28 Appareil de traitement d'excréments et d'urines conçu pour toilettes écologiques sans chasse WO2008034337A1 (fr)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
CN200610112850.6 2006-09-05
CNB2006101128506A CN100540655C (zh) 2006-09-05 2006-09-05 一株嗜氨基氨基杆菌及其菌剂与应用
CN200710142813.4 2007-07-30
CN2007101428134A CN101139555B (zh) 2007-07-30 2007-07-30 一株酿酒酵母及其复合菌剂的应用
CN200710143603.7 2007-08-14
CN200710143603 2007-08-14

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WO2008034337A1 true WO2008034337A1 (fr) 2008-03-27

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CN105368748A (zh) * 2015-12-04 2016-03-02 无锡拓能自动化科技有限公司 一种城市污水处理复合菌剂及其制备方法
CN106831033A (zh) * 2017-03-28 2017-06-13 湖南天爱农业科技有限公司 一种利用秸秆与尿液混合发酵的制肥装置
CN108315270A (zh) * 2018-01-04 2018-07-24 四川缪氏现代农业发展有限公司 鲜食葡萄蒸馏酒酿酒酵母及其应用
CN109252572A (zh) * 2017-07-14 2019-01-22 上海百铭信息科技有限公司 一种无水马桶
CN112812990A (zh) * 2020-12-31 2021-05-18 宜兴艾科森生态环卫设备有限公司 一种用于***物处理的复合微生物菌剂及应用和复配方法
CN113349668A (zh) * 2021-06-04 2021-09-07 森诺技术有限公司 一种家用免水冲智能环保厕具的控制方法

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CN105368748A (zh) * 2015-12-04 2016-03-02 无锡拓能自动化科技有限公司 一种城市污水处理复合菌剂及其制备方法
CN106831033A (zh) * 2017-03-28 2017-06-13 湖南天爱农业科技有限公司 一种利用秸秆与尿液混合发酵的制肥装置
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CN108315270A (zh) * 2018-01-04 2018-07-24 四川缪氏现代农业发展有限公司 鲜食葡萄蒸馏酒酿酒酵母及其应用
CN112812990A (zh) * 2020-12-31 2021-05-18 宜兴艾科森生态环卫设备有限公司 一种用于***物处理的复合微生物菌剂及应用和复配方法
CN113349668A (zh) * 2021-06-04 2021-09-07 森诺技术有限公司 一种家用免水冲智能环保厕具的控制方法

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