TWI750621B - Bio-stimulating agricultural agent and method for producing the same - Google Patents
Bio-stimulating agricultural agent and method for producing the same Download PDFInfo
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本發明係有關一種兼具生物刺激素特性之農作用劑及其製備方法，係一種農業用生物產品及其製程者。 The present invention relates to an agricultural acting agent with biostimulant properties and a preparation method thereof, and relates to an agricultural biological product and its preparation process.
一般在栽種農作物時，為提升土壤肥力、抵抗害蟲、增進生長等因素，多會施用肥料與農藥。 Generally, when planting crops, in order to improve soil fertility, resist pests, and enhance growth, fertilizers and pesticides are often applied.
然許多肥料或農藥皆含有合成化學成份，長期使用後可能會造成地力退化、土壤酸化、植物荷爾蒙改變等，其後遺症最終可能會使得農地不適耕種；而若以天然原料來作施肥或害蟲防制，有時效果則不盡理想。 However, many fertilizers or pesticides contain synthetic chemical components, which may cause soil fertility degradation, soil acidification, and changes in plant hormones after long-term use. The sequelae may eventually make farmland unsuitable for cultivation. , sometimes the effect is not ideal.
有鑑於上述缺失弊端，本發明人認為具有改正之必要，遂以從事相關技術以及產品設計製造之多年經驗，秉持優良設計理念，針對以上不良處加以研究創作，在經過不斷的努力後，終乃推出本發明兼具生物刺激素特性之農作用劑及其製備方法，其以更正產品結構以提升產品優良之功效。 In view of the above shortcomings, the inventor believes that it is necessary to make corrections, so with years of experience in related technologies and product design and manufacturing, and adhering to good design concepts, research and creation for the above shortcomings, after continuous efforts, finally The agricultural acting agent with biostimulant properties and the preparation method thereof of the present invention are introduced, which can correct the product structure to improve the excellent efficacy of the product.
本發明兼具生物刺激素特性之農作用劑及其製備方法之主要目的係提供一種以微藻天然發酵製成，使用上無不良後果且對作物具顯著有益效果的生物性農作用劑及其製程者。 The main purpose of the agricultural agent having both biostimulant properties and the preparation method thereof of the present invention is to provide a biological agricultural agent which is made by natural fermentation of microalgae, has no adverse consequences and has significant beneficial effects on crops and the same. Processer.
為達到前揭之目的，本發明兼具生物刺激素特性之農作用劑的製備方法包括有以下步驟：製備第一菌液，將第一種真菌依序進行平盤培養與液態培養後，得到第一菌液；製備第二菌液，將第二種真菌依序進行平盤培養與液態培養後，得到第二菌液；製備第三菌液，將第三種真菌依序進行平盤培養與液態培養後，得到第三菌液；製備藻液，將微藻藻粉與純水混合成藻液；一次發酵，將第一菌液加入藻液進行發酵；二次發酵，一次發酵完成後進行過濾分離以得到藻渣與一次發酵液，接著將第二菌液、第三菌液與純水混合藻渣後再次進行發酵；混液，再次過濾分離以得到二次發酵液，將所得的一次發酵液與二次發酵液混合即為本發明之農作用劑。 In order to achieve the purpose disclosed above, the preparation method of the agricultural acting agent with biostimulant characteristics of the present invention comprises the following steps: preparing a first bacterial liquid, carrying out the plate culture and liquid culture of the first fungus in sequence, and obtaining The first bacterial solution; the second bacterial solution is prepared, and the second fungus is sequentially subjected to flat plate culture and liquid culture to obtain the second bacterial solution; the third bacterial solution is prepared, and the third fungus is sequentially subjected to flat plate culture After culturing with the liquid state, the third bacterial liquid is obtained; for preparing the algal liquid, the microalgae algal powder and pure water are mixed to form the algal liquid; for the first fermentation, the first bacterial liquid is added to the algal liquid for fermentation; for the second fermentation, after the first fermentation is completed Carry out filtration and separation to obtain algae residue and primary fermentation liquid, and then ferment again after mixing the second bacterial liquid, the third bacterial liquid and pure water with the algal residue; the mixed liquid is filtered and separated again to obtain the secondary fermentation liquid, and the obtained primary The mixing of the fermentation broth and the secondary fermentation broth is the agricultural agent of the present invention.
其中，第一種真菌為杏鮑菇菌(Pleurotus eryngii)；第二與第三種真菌分別為假絲酵母菌(Pseudozyma aphidis)與米麴菌(Aspergillus oryzae)；微藻藻粉則為普通小球藻(Chlorella vulgaris)藻粉。 Among them, the first fungus is Pleurotus eryngii ; the second and third fungi are Pseudozyma aphidis and Aspergillus oryzae respectively ; Chlorella vulgaris algal flour.
製備步驟中，一次發酵的目的是為了藉由杏鮑菇菌的纖維素酶來分解微藻的細胞壁，以得到水解蛋白、水解胺基酸、游離胺基酸、活性胜肽與小球藻生長因子等有效成份，二次發酵的目的則除了進一步獲取殘留在藻渣內的有效成份外，因假絲酵母菌與米麴菌還可分泌β-葡萄糖苷酶、脂肪酸轉化酶、胡蘿蔔素合成酶等酶素，可產生複合發酵反應，以提 高多元不飽和脂肪酸ω-3型PUFAs(如DHA、EPA和α-LA)及食用光合色素(如蝦青素Astaxanthin與葉黃素Lutein)等產量。 In the preparation step, the purpose of a fermentation is to decompose the cell wall of the microalgae by the cellulase of Pleurotus eryngii to obtain hydrolyzed proteins, hydrolyzed amino acids, free amino acids, active peptides and chlorella growth. Factors and other active ingredients, the purpose of secondary fermentation is not only to further obtain the active ingredients remaining in the algal residue, because Candida and rice koji can also secrete β-glucosidase, fatty acid invertase, carotene synthase and other enzymes, which can produce a complex fermentation reaction to improve High yield of polyunsaturated fatty acid omega-3 PUFAs (such as DHA, EPA and α-LA) and edible photosynthetic pigments (such as Astaxanthin and Lutein).
而將本發明之農作用劑實際噴灑於農作物葉面以及灌注於土壤後發現，其確實可以調節土壤理化性質與提升作物生長而具生物刺激素特性；而與以往的合成化學肥料與農藥相比，本農作用劑是以微藻藻粉配合菌液天然發酵製成，不會對農地與農作物產生不良後果，而若與天然的肥料與害蟲防制物相比，本農作用劑將微藻經二次發酵，可有效提升其營養價值，在改善土壤理化性質、促進作物生長、保護作物等功效上有顯著的效果，為具有進步性的產品。 After actually spraying the agrochemical agent of the present invention on the foliage of crops and pouring it into the soil, it is found that it can indeed adjust the physical and chemical properties of the soil and enhance the growth of crops, and has biostimulant properties; compared with the previous synthetic chemical fertilizers and pesticides , This agricultural agent is made of microalgae algal powder combined with bacterial liquid natural fermentation, which will not have adverse consequences on farmland and crops, and if compared with natural fertilizers and pest control materials, this agricultural agent will microalgae. After secondary fermentation, it can effectively enhance its nutritional value, and has significant effects in improving soil physical and chemical properties, promoting crop growth, and protecting crops. It is a progressive product.
1:製備第一菌液 1: Prepare the first bacterial solution
2:製備第二菌液 2: Prepare the second bacterial solution
3:製備第三菌液 3: Preparation of the third bacterial solution
4:製備藻液 4: Preparation of algal fluid
5:一次發酵 5: One fermentation
6:二次發酵 6: Secondary fermentation
7:混液 7: Mixed liquid
第一圖係本發明兼具生物刺激素特性之農作用劑的製備方法之步驟流程圖。 The first figure is a flow chart of the steps of the preparation method of the agricultural acting agent with biostimulant properties of the present invention.
第二圖係本發明兼具生物刺激素特性之農作用劑用於小白菜之試驗結果圖。 The second graph is a graph of the test results of the agricultural agent with biostimulant properties of the present invention applied to Chinese cabbage.
第三圖係本發明兼具生物刺激素特性之農作用劑用於甘藍菜之試驗結果圖。 The third figure is a graph of the test results of the agricultural agent with biostimulant properties of the present invention applied to cabbage.
第四圖係本發明兼具生物刺激素特性之農作用劑用於蘿蔔之試驗結果圖。 The fourth figure is a graph of the test results of the present invention's agricultural agent with biostimulant properties applied to radish.
第五圖係本發明兼具生物刺激素特性之農作用劑用於黃秋葵之試驗結果圖。 The fifth figure is a graph of the test results of the agricultural agent with biostimulant properties of the present invention applied to okra.
第六圖係本發明兼具生物刺激素特性之農作用劑施用於百香果之成果圖。 The sixth figure is the result of applying the agricultural agent with biostimulant properties of the present invention to passion fruit.
第七圖係本發明兼具生物刺激素特性之農作用劑施用於玉女番茄之成果圖。 The seventh figure is the result of applying the agricultural agent with biostimulant properties of the present invention to Yunu tomato.
第八圖係本發明兼具生物刺激素特性之農作用劑施用於玉米之成果圖。 The eighth figure shows the results of the application of the agricultural agent with biostimulant properties to corn of the present invention.
第九圖係本發明兼具生物刺激素特性之農作用劑施用於高麗菜之成果圖。 The ninth figure shows the result of applying the agricultural agent with biostimulant properties of the present invention to cabbage.
第十圖係本發明兼具生物刺激素特性之農作用劑施用於大北蕉之成果圖。 Figure 10 shows the results of the application of the agricultural agent with biostimulant properties to Dabei banana of the present invention.
一、製備方法 1. Preparation method
〔請參閱第一圖〕本發明兼具生物刺激素特性之農作用劑的製備方法包括以下步驟：製備第一菌液(1)，先將成份為葡萄糖20g/L、酵母萃取物6g/L、麥芽15g/L、洋菜膠10g/L的固態培養基倒入培養盤，每100ml固態培養基約可倒入4~5個培養盤，接著從一長滿杏鮑菇菌(Pleurotus eryngii)的菌盤夾出小菌塊並將其放入固態培養基內，後以溫度20℃~25℃、濕度60~70%進行平盤培養10~14天，平盤培養完畢後改為液態培養，先將長有菌的固態培養基加入無菌水並均質絞碎，每3盤固態培養基約加入250~300ml的無菌水，接著將絞碎的菌液加入液態培養基，液態培養基成份為葡萄糖20g/L、酵母萃取物6g/L、麥芽15g/L，加入菌液與液態培養基的體積比例為1：2，後以溫度25℃進行液態培養3~5天，培養期間持續搖擺振盪，培養完成後即為第一菌液；製備第二菌液(2)，此步驟大致與製備第一菌液(1)相同，同樣為先進行平盤培養再改為液態培養，培養基成份及培養條件也與製備第一菌液(1)時相同，差別在於要培養的菌種為假絲酵母菌(Pseudozyma aphidis)，培養完成後即為第二菌液；製備第三菌液(3)，此步驟也大致與製備第一菌液(1)相同，同樣為先進行平盤培養再改為液態培養，培養基成份及培養條件也與 製備第一菌液(1)時相同，差別在於要培養的菌種為米麴菌(Aspergillus oryzae)，培養完成後即為第三菌液；製備藻液(4)，將1kg普通小球藻(Chlorella vulgaris)藻粉與10L純水均勻混合而得藻液，接著將藻液以121℃滅菌30分鐘後再放涼至室溫；一次發酵(5)，將900ml的第一菌液加入藻液中，接著以溫度25℃~37℃發酵5~7天，發酵期間持續打氣；二次發酵(6)，一次發酵(5)完成後進行過濾分離，以得到藻渣以及一次發酵液，後將第二菌液及第三菌液各450ml、10L純水與藻渣混合，接著再次以溫度25℃~37℃發酵3~5天，發酵期間同樣持續打氣；混液(7)，二次發酵(6)完成後進行過濾分離以得到二次發酵液，後將所得的一次發酵液與二次發酵液全部混合，即為本發明的兼具生物刺激素特性之農作用劑。 [Please refer to the first figure] The preparation method of the agricultural agent with biostimulant properties of the present invention includes the following steps: to prepare the first bacterial solution (1), firstly, the ingredients are glucose 20g/L, yeast extract 6g/L malt 15g / L, agar gum 10g / L of solid medium was poured into culture dishes, each of about 100ml solid medium may be poured into a 4 to 5 culture dishes, then covered from Pleurotus (Pleurotus eryngii) of Bacteria plate clips out small bacteria and puts them into solid medium, and then carries out flat plate culture at a temperature of 20℃~25℃ and a humidity of 60~70% for 10~14 days. After the flat plate culture is completed, it is changed to liquid culture. The solid medium with bacteria is added to sterile water and homogeneously minced, and the sterile water of about 250~300ml is added to every 3 plates of solid medium, and then the minced bacterial liquid is added to the liquid medium, and the liquid medium components are glucose 20g/L, yeast Extract 6g/L, malt 15g/L, add bacteria liquid and liquid medium in a volume ratio of 1:2, and then carry out liquid culture at a temperature of 25°C for 3 to 5 days. The first bacterial liquid; the preparation of the second bacterial liquid (2), this step is roughly the same as the preparation of the first bacterial liquid (1), and the same is to first carry out flat plate culture and then change to liquid culture, and the medium components and culture conditions are also the same as the preparation of the first bacterial liquid (1). The first bacterial solution (1) is the same, the difference is that the bacterial species to be cultivated is Pseudozyma aphidis , which is the second bacterial solution after the cultivation is completed; the third bacterial solution (3) is prepared, and this step is also roughly the same as The preparation of the first bacterial liquid (1) is the same, and the same is to first carry out flat plate culture and then change to liquid culture, and the medium components and culture conditions are also the same as when preparing the first bacterial liquid (1), the difference is that the bacterial species to be cultivated is rice. Koji bacteria (Aspergillus oryzae ), is the third bacterial liquid after the cultivation is completed; prepare the algal liquid (4), uniformly mix 1 kg of Chlorella vulgaris (Chlorella vulgaris) algal powder with 10 L of pure water to obtain the algal liquid, and then mix the algal liquid The liquid was sterilized at 121°C for 30 minutes and then cooled to room temperature; once fermentation (5), 900ml of the first bacterial liquid was added to the algae liquid, and then fermented at a temperature of 25°C to 37°C for 5 to 7 days, and continued to pump during the fermentation Secondary fermentation (6), filter separation after primary fermentation (5) is completed, to obtain algal slag and a fermented liquid, respectively 450ml, 10L of pure water and algal slag are mixed with the second bacterial liquid and the third bacterial liquid afterward, Then again ferment at a temperature of 25°C to 37°C for 3 to 5 days, and continue to pump gas during the fermentation; Mixed liquid (7), after the secondary fermentation (6) is completed, filter and separate to obtain a secondary fermentation liquid, and then the obtained primary fermentation The liquid and the secondary fermentation liquid are all mixed together, that is, the agricultural agent with the characteristics of biostimulants of the present invention.
本農作用劑，用於土壤調節時(未有作物種植之情況)稀釋300~500倍為佳，用於作物根系澆灌時稀釋600~800倍為佳，用於葉面噴灑時稀釋1000~1200倍為佳。 This agricultural action agent is preferably diluted 300-500 times when used for soil conditioning (without crop planting), preferably 600-800 times when used for crop root irrigation, and 1000-1200 times when used for foliar spraying Double is better.
二、土壤理化性質調節試驗： Second, soil physical and chemical properties adjustment test:
選定兩塊地質等各項條件皆相近的土場，並分別施用本農作用劑與清水來作對照，對照組每10m2僅灌注150L水體，實驗組則額外在水體內添加30ml本農作用劑，每7天施用一次，共施作4次，並於第0週(未灌注前)至第4週採土檢驗，檢驗內容包括pH值、電導度、鹽度、鉀離子、鈣離子，檢驗結果如下列。 The conditions are selected dump two similar geological and agricultural agents are administered with the water present to make a control group only 10m 2 per 150L water reperfusion, an additional experimental group present in the body of water is added 30ml agricultural agent , once every 7 days, a total of 4 times, and from the 0th week (before perfusion) to the 4th week, the soil was collected for inspection. The inspection contents included pH value, conductivity, salinity, potassium ion, calcium ion. The results are as follows.
1-pH值 1-pH
表1
結果分析：大部分作物之最佳生長環境為弱酸性質土壤(約落在pH值5.5-6.5)，其土壤物理性、化學性及生物性(土壤菌相)能夠達到最好的平衡條件，本試驗進行前的土壤酸鹼值偏向弱鹼pH 8.0-8.2，透過施用本農作用劑後，土壤酸鹼值明顯改善至pH值標準範圍5.5-7.5以內。 Result analysis: The best growth environment for most crops is weakly acidic soil (about pH 5.5-6.5), and the soil physical, chemical and biological properties (soil bacteria phase) can achieve the best balance conditions. Before the experiment, the soil pH value tended to be weak base pH 8.0-8.2. After the application of this agricultural agent, the soil pH value was significantly improved to within the standard pH value range of 5.5-7.5.
2-電導度 2 - Conductivity
結果分析：最適合大部分作物生長的土壤電導度數值為0.3-0.6(dS/m)，經過第一週施灑本農作用劑後，即快速且有效的將實驗組土壤提升至電導度標準值以上(0.33dS/m)，並使後續三週分別維持在0.34(dS/m)、0.33(dS/m)、0.32(dS/m)的最佳土壤電導度，反之對照組未能有效提升土壤電導度，代表土壤中水溶性鹽類的濃度偏低，可能造成作物的養分轉換率不佳。 Analysis of the results: The soil conductivity value most suitable for the growth of most crops is 0.3-0.6 (dS/m). After the first week of application of this agricultural agent, the soil in the experimental group was quickly and effectively improved to the conductivity standard. value above (0.33dS/m), and maintained at the optimum soil conductivity of 0.34 (dS/m), 0.33 (dS/m), and 0.32 (dS/m) for the following three weeks respectively. Otherwise, the control group was not effective. Increasing soil conductivity means that the concentration of water-soluble salts in the soil is low, which may result in poor nutrient conversion rates for crops.
3-鹽度 3 - Salinity
表3
結果分析：土壤鹽害等級可分為「無」、「輕微」、「中等」、「高度」、「極度」，鹽含量(%)數值
4-鉀離子 4-potassium ion
結果分析：適合耕種及作物生長的土壤有效鉀標準值為30-100(ppm)，鉀離子可以促進植物根系的生長，經過第一週施用本農作用劑的土壤能夠明顯提升鉀離子至標準值內(87ppm)，並能在後續三週維持土壤中有效鉀的濃度(分別為92ppm、95ppm、90ppm)，反之對照組未能有效提高土壤中的有效鉀。 Result analysis: The standard value of available potassium in soil suitable for cultivation and crop growth is 30-100 (ppm), and potassium ions can promote the growth of plant roots. After the first week of application of this agricultural agent, the soil can significantly increase potassium ions to the standard value. (87ppm), and could maintain the concentration of available potassium in the soil (92ppm, 95ppm, 90ppm, respectively) in the following three weeks, whereas the control group failed to effectively increase the available potassium in the soil.
5-鈣離子 5-Calcium ion
表5
結果分析：健康且具有肥力的土壤，其中有效鈣的標準值應為57-1145(ppm)，缺鈣的土壤其原因很多，最為可能的是磷肥中有效性磷非常容易與土壤中的正離子(鈣、鐵、鋁、鎂等)結合，形成不易溶的磷化合物，如磷酸鈣、磷酸鐵、磷酸鋁等，在農田中累積，但作物並不易利用，而施灑本農作用劑的第一週即有效的轉化土壤中的鈣離子，並提升鈣離子濃度至標準值內(79ppm)，更於第二週至第四週顯著的正向提高(分別為83ppm、110ppm、140ppm)，後續的耕種期能使作物有效利用土壤中的鈣離子，反之對照組的鈣離子僅介於最低標準值界線，土壤肥力明顯不足。 Result analysis: In healthy and fertile soil, the standard value of available calcium should be 57-1145 (ppm). There are many reasons for calcium-deficient soil. (calcium, iron, aluminum, magnesium, etc.) combine to form insoluble phosphorus compounds, such as calcium phosphate, iron phosphate, aluminum phosphate, etc., which accumulate in farmland, but the crops are not easy to use, and the first step of applying this agricultural agent It can effectively convert calcium ions in the soil within one week, and increase the calcium ion concentration to within the standard value (79ppm), and it has a significant positive increase (83ppm, 110ppm, 140ppm, respectively) from the second week to the fourth week. During the cultivation period, the crops can effectively utilize the calcium ions in the soil. On the contrary, the calcium ions in the control group are only at the boundary of the minimum standard value, and the soil fertility is obviously insufficient.
三、作物試驗 3. Crop test
將本發明之農作用劑施用於不同作物，並皆於播種後第5週記錄生長結果，對照組只以清水噴灑於葉面與灌注於土壤，實驗組則額外在清水添加本農作用劑，稀釋800倍，各種作物的試驗結果如下列，其中小白菜與甘藍菜的實驗組在莖高與葉長度都有所提升，而蘿蔔與黃秋葵的實驗組於植株的蛀蟲顯著減少。 The agricultural agent of the present invention was applied to different crops, and the growth results were recorded in the 5th week after sowing. The control group was only sprayed with clear water on the foliage and poured into the soil, and the experimental group was additionally added with the agricultural agent in clear water. Diluted 800 times, the test results of various crops are as follows. The experimental group of cabbage and cabbage has increased stem height and leaf length, while the experimental group of radish and okra has significantly reduced plant borers.
1-小白菜〔請一併參閱第二圖〕：
2-甘藍菜〔請一併參閱第三圖〕 2- Cabbage (please also refer to the third picture)
3-蘿蔔〔請一併參閱第四圖〕 3- Radish (please refer to the fourth picture together)
4-黃秋葵〔請一併參閱第五圖〕 4- Okra (please refer to the fifth picture together)
四、農友實際使用 Fourth, the actual use of farmers
將本農作用劑實際提供給農友使用，並回饋使用結果。 Provide this agricultural agent to farmers for use, and give feedback on the results of use.
1-百香果〔請一併參閱第六圖〕 1-Passion fruit (please refer to the sixth picture together)
1月中開始施用本農作用劑，對照組僅施用基肥，實驗組則以基肥加上本農作用劑稀釋500倍做葉面噴灑，7-10天用1次，同年2月底發現實驗組的百香果生長速度較快，植株強健，高度約為對照組的2倍高。 In the middle of January, the application of the agricultural agent was started. The control group was only applied with basal fertilizer. The experimental group was sprayed with the basic fertilizer plus the agricultural agent diluted 500 times for foliar spraying, once every 7-10 days. Passion fruit grew faster, and the plants were robust, and the height was about twice as high as that of the control group.
2-玉女番茄〔請一併參閱第七圖〕 2-Jade Maiden Tomato (please refer to the seventh picture together)
12月底開始施用本農作用劑，初期稀釋800倍作葉面噴灑，中期稀釋500倍作噴灑，皆7-10天用1次，施用時氣候異常高溫，再加上時而淹水時而缺少水分的情況下，隔年2月發現對照組出現瘋叢的情況，葉片蜷曲、植株矮小，而實驗組的玉女番茄則仍然強健，葉子翠綠，生長速度提升約5-10%。 Begin to apply this agricultural agent at the end of December. The initial dilution is 800 times for foliar spraying, and the intermediate dilution is 500 times for spraying. Both are used once every 7-10 days. The climate is abnormally high during application, and sometimes flooded and sometimes lack of water. In the case of water, the control group was found to have a crazy bush in February of the next year, with curled leaves and dwarf plants, while the Yunu tomato in the experimental group was still strong, with emerald green leaves, and the growth rate increased by about 5-10%.
3-玉米〔請一併參閱第八圖〕 3-Corn (please refer to the eighth picture together)
除基肥外，對照組以清水噴灑葉面，實驗組則以本農作用劑稀釋500倍噴灑於葉面上，從圖可看到實驗組的成長速度較對照組快，實驗組玉米葉子較大約1.5倍大，莖也粗了約1倍。 Except for basal fertilizer, the control group was sprayed with water on the leaves, while the experimental group was sprayed with a 500-fold dilution of this agricultural agent on the leaves. It can be seen from the figure that the growth rate of the experimental group was faster than that of the control group, and the corn leaves of the experimental group were approximately 1.5 times larger, and the stem is also about 1 times thicker.
4-高麗菜〔請一併參閱第九圖〕 4-Korean cabbage (please also refer to the ninth picture)
除基肥外，對照組以清水做葉面噴灑，實驗組則施灑稀釋500倍的本農作用劑於葉面，相比於對照組，實驗組的蟲蛀比例明顯較低。 In addition to basal fertilizer, the control group was sprayed with clear water, and the experimental group was sprayed with a 500-fold dilution of the agricultural agent on the leaves. Compared with the control group, the proportion of insects in the experimental group was significantly lower.
5-大北蕉〔請一併參閱第十圖〕 5-Da Beijiao [please refer to the tenth picture together]
對照組與實驗組各10顆，除基肥外，實驗組以稀釋200倍的本農作用劑灌注於土壤，以及稀釋500倍的本農作用劑噴灑於葉面，每7天施用1次共4次，四週後發現對照組中的3顆出葉片短小、捲曲、枯黃的情況，而實驗組的葉片則皆大而翠綠。 The control group and the experimental group each had 10 grains. Except for the base fertilizer, the experimental group was perfused with a 200-fold dilution of the agricultural agent in the soil, and the 500-fold dilution of the agricultural agent was sprayed on the foliage, once every 7 days for a total of 4 Second, after four weeks, 3 leaves in the control group were found to be short, curled, and withered yellow, while the leaves in the experimental group were all large and emerald green.
唯以上所述者，僅為本發明之一較佳實施例而已，當不能以之限定本發明之範圍。即大凡依申請專利範圍所作之均等變化與修飾，皆應仍屬本創作專利涵蓋之範圍內。 Only the above-mentioned is only a preferred embodiment of the present invention, and should not be used to limit the scope of the present invention. That is to say, all equivalent changes and modifications made in accordance with the scope of the patent application should still fall within the scope of the invention patent.
綜上所述，當知本發明具有新穎性、進步性，且本發明未見之於任何刊物，當符合專利法第22條之規定。 To sum up, when it is known that the present invention is novel and progressive, and the present invention has not been seen in any publications, it shall comply with the provisions of Article 22 of the Patent Law.
1:製備第一菌液1: Prepare the first bacterial solution
2:製備第二菌液2: Prepare the second bacterial solution
3:製備第三菌液3: Preparation of the third bacterial solution
4:製備藻液4: Preparation of algal fluid
5:一次發酵5: One fermentation
6:二次發酵6: Secondary fermentation
7:混液7: Mixed liquid
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104261981A (en) * | 2014-09-19 | 2015-01-07 | 南京农业大学 | Preparation method of seaweed organic fertilizer and organic fertilizer prepared by using method |
| TW201824996A (en) * | 2017-01-11 | 2018-07-16 | 台建生技股份有限公司 | Soil improving composition and method for promoting plant growth which not only has a rapid and long-acting nitrogen supplementation, but also inhibits the occurrence of pests and diseases |
| TW201825441A (en) * | 2017-01-11 | 2018-07-16 | 台建生技股份有限公司 | Use of algae broken-wall fermentate as foliar fertilizer and enhancing leaf crops against insects capable of being applied to crops or plants to promote the growth of stems, leaves and/or fruits thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104261981A (en) * | 2014-09-19 | 2015-01-07 | 南京农业大学 | Preparation method of seaweed organic fertilizer and organic fertilizer prepared by using method |
| TW201824996A (en) * | 2017-01-11 | 2018-07-16 | 台建生技股份有限公司 | Soil improving composition and method for promoting plant growth which not only has a rapid and long-acting nitrogen supplementation, but also inhibits the occurrence of pests and diseases |
| TW201825441A (en) * | 2017-01-11 | 2018-07-16 | 台建生技股份有限公司 | Use of algae broken-wall fermentate as foliar fertilizer and enhancing leaf crops against insects capable of being applied to crops or plants to promote the growth of stems, leaves and/or fruits thereof |
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