TWI692305B - Soil improvement composition and method for promoting plant growth - Google Patents
Soil improvement composition and method for promoting plant growth Download PDFInfo
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Abstract
本發明提供一種土壤改良組成物及其促進植物生長的方法,利用益生 菌混合液以及藻類破壁發酵物原液所組成的土壤改良組成物,可改變土壤中微生物菌相、酸鹼值、導電度、有效性成分等特性,提升土壤的有機物量及土壤的生物效力,進而使植物根系的吸收效能獲得改善,使其耕作時不但具快速與長效的補氮作用,更可抑制病蟲害的發生。 The invention provides a soil improvement composition and a method for promoting plant growth, using probiotics The soil improvement composition composed of the mixed liquid of bacteria and the original liquid of the algae wall-breaking fermentation product can change the characteristics of the microbial flora, pH value, electrical conductivity, and effective components in the soil, and improve the amount of organic matter in the soil and the biological effectiveness of the soil. In turn, the absorption efficiency of plant roots is improved, which not only has rapid and long-term nitrogen supplementation during cultivation, but also suppresses the occurrence of diseases and insect pests.
Description
本發明係關於一種可用以改良土壤成分的土壤改良組成物,尤其是關於一種包括由微藻破壁後所收集之發酵物加上益生菌的土壤改良組成物,並藉由該組成物促進植物生長的方法。 The present invention relates to a soil improvement composition that can be used to improve the soil composition, in particular to a soil improvement composition including a fermented substance collected after the breaking of microalgae and probiotics, and promoting the plant by the composition Method of growth.
一般情況下,無論是經濟作物例如禾穀類、瓜果類、蔬菜類或是觀賞植物盆栽,為使其開花數多、結果數多而品質好,肥料的使用是最常見且方便的方法。傳統上最常使用的肥料性質上屬於化學肥料,但化學肥料肥效單一、容易造成環境污染,長期使用更會導致土壤被破壞,使土質不再適合作物或植株生長。因此,為使農業能夠永續經營,不依賴大量化學肥料及化學農藥的農業生產體系是必要的,如此才能減少對環境生態的衝擊,此外,生產的能源及成本可以降低,但仍可維持穩定產量,甚至提高收益更是永續農業所追求的目標。 In general, whether it is cash crops such as cereals, melons, vegetables, or ornamental plants, the use of fertilizers is the most common and convenient method in order to make the number of flowering and fruiting and good quality. The most commonly used fertilizers traditionally belong to chemical fertilizers, but chemical fertilizers have a single fertilizer effect and are easy to cause environmental pollution. Long-term use will also cause soil damage and make the soil no longer suitable for the growth of crops or plants. Therefore, in order to enable the sustainable operation of agriculture, an agricultural production system that does not rely on a large amount of chemical fertilizers and chemical pesticides is necessary, so as to reduce the impact on the environment and ecology. In addition, the energy and cost of production can be reduced, but it can still maintain stability Yield and even increased income are the goals pursued by sustainable agriculture.
農業生產若不依賴化學肥料,就需替代以生物性肥料、有機質肥料及礦物質肥料,但以生物性肥料促進化學、有機質肥料利用效率之技術則是目前最受重視的土壤生物技術。最早的生物性肥料,是農民將蔬果採收完畢後,再焚地耕種,近年來則是利用豆科植物與穀類作物進行輪作。生物性肥料係指含具有活性微生物體(包含其休眠孢子),如細菌、真菌、藻類等微生物之微生物體及其代謝產物的特定製劑,其具有供應植物養分的效果,例如增進植物養分和元素之供應量與總量、刺激植物生長、或促進植物對營養和元素的吸收。因此,發現並利用一含有豐富前述有效成分 且易取得或保存之生物性天然有機肥料,將是土壤生物技術發展的一重要課題。 If agricultural production does not depend on chemical fertilizers, bio-fertilizers, organic fertilizers and mineral fertilizers need to be replaced, but the technology of using bio-fertilizers to promote the utilization efficiency of chemical and organic fertilizers is currently the most valued soil biotechnology. The earliest biological fertilizers were farmers who harvested vegetables and fruits and then burned them for cultivation. In recent years, they have used legumes and cereal crops for rotation. Biological fertilizer refers to a specific preparation containing microorganisms and metabolites of microorganisms with active microorganisms (including their dormant spores), such as bacteria, fungi, algae, etc., which have the effect of supplying plant nutrients, such as enhancing plant nutrients and elements Supply and total quantity, stimulate plant growth, or promote plant absorption of nutrients and elements. Therefore, find and use a rich The biological natural organic fertilizer that is easy to obtain or preserve will be an important issue in the development of soil biotechnology.
此外,因為精緻化農業的推廣使得土地被過度使用,土壤的性質產生改變、導致地力衰退,或是因為過度施肥、農藥使用所造成的土壤毒化,又或是長期休耕、廢置使土壤硬化、有機成分改變的情況下,即使可於後續施加效用良好的肥料進行補強,但先天土質不良的情況下,肥料施加之效果有限,而且容易造成植物賀爾蒙的變化,影響作物產量甚鉅。因此,若能開發出一種特殊的土壤改良配方,用於提升土壤有機物量及生物效力,兼具土壤改良與養分提供之效能,不但可恢復耕地栽種的基本能力,更可提高作物產量或品質,達成土地復育的目的,進而實現永續農業的觀念和有機農業的槪念。 In addition, because of the promotion of refined agriculture, the land is overused, the nature of the soil is changed, resulting in decline in fertility, or the soil is poisoned by excessive fertilization and pesticide use, or it is caused by long-term fallow and waste hardening the soil, organic When the composition is changed, even if it can be reinforced by the subsequent application of a good-effect fertilizer, the effect of fertilizer application is limited when the congenital soil quality is poor, and it is easy to cause changes in plant hormones and affect the crop yield. Therefore, if a special soil improvement formula can be developed to increase the amount of soil organic matter and biological effectiveness, and combine the effectiveness of soil improvement and nutrient supply, not only can the basic ability of cultivated land cultivation be restored, but also the crop yield or quality can be improved. Achieve the purpose of land restoration, and then realize the concept of sustainable agriculture and the idea of organic agriculture.
本發明目的之一在於提供一種能夠將土壤三相進行調整,進而改變土壤成分與其他特性的土壤改良組成物。藉由本發明的組成物,可使土壤中的微生物相獲得改善,藉其作用改變了原有的酸鹼值、導電度、有機質含量,以及磷、鉀、鈣等有效性成分含量,並可抑制土壤中的病原菌,使植物能在合適、有利的土壤成分下生長,最終獲致高產量與高品質的作物產物。 One of the objects of the present invention is to provide a soil improvement composition capable of adjusting the three-phase soil and further changing the soil composition and other characteristics. With the composition of the present invention, the microbial phase in the soil can be improved, by which the original pH value, electrical conductivity, organic matter content, and the content of effective ingredients such as phosphorus, potassium, and calcium can be changed, and can be suppressed Pathogens in the soil enable plants to grow under suitable and favorable soil components, resulting in high-yield and high-quality crop products.
本發明的另一目的則在於改善土壤成分同時,提供植物生長所需的有機營養物質,亦即不單是改良土壤成分,更同時使其含有更多營養成分,使於將來耕作時,讓植物的生長更為迅速而茁壯。 Another object of the present invention is to improve the soil composition while providing the organic nutrients needed for plant growth, that is, not only to improve the soil composition, but also to contain more nutrients at the same time, so that in future cultivation, the plant The growth is more rapid and robust.
為了達成前述的目的,本發明提供一種土壤改良組成物,包括:一藻類破壁發酵物原液;一益生菌混合液;以及水,其中,該益生菌混合液中之菌體係包括:光合菌(Photosynthetic bacteria)、硝化菌(Nitrifying bacteria)、枯草桿菌(Bacillus subtilis)、乳酸菌(Lactobacillus)及放線菌(Actinobacteria)。 In order to achieve the foregoing object, the present invention provides a soil improvement composition comprising: a stock solution of algae wall-breaking fermentation product; a probiotic mixed solution; and water, wherein the bacterial system in the probiotic mixed solution includes: photosynthetic bacteria ( photosynthetic bacteria), nitrifying bacteria (nitrifying bacteria), Bacillus subtilis (Bacillus subtilis), lactic acid bacteria (Lactobacillus) and actinomycetes (Actinobacteria).
在本發明的一實施例中,該藻類破壁發酵物係由以下方法製得:將一預定數量之微藻加入一預先培養且與該微藻具一預定比例之菌體溶液中進行發酵,其中,該菌體溶液中之菌體可分泌纖維素水解酵素;該微藻加入後使該菌體分泌該纖維素水解酵素以水解該微藻之細胞壁;以及收集該微藻於細胞壁水解後所形成之一藻類破壁發酵物原液。 In an embodiment of the present invention, the algae wall-breaking fermentation product is prepared by the following method: adding a predetermined amount of microalgae to a pre-cultured microbial cell solution having a predetermined ratio with the microalgae for fermentation, Wherein, the bacterial cells in the bacterial cell solution can secrete cellulose hydrolyzing enzyme; after the microalgae is added, the bacterial cell secretes the cellulose hydrolyzing enzyme to hydrolyze the cell wall of the microalgae; and the microalgae is collected after the cell wall is hydrolyzed Formed one of the stock solutions of algae wall breaking ferment.
在本發明一實施例的一態樣中,所述之土壤改良組成物,其中該菌體可為細菌或真菌。 In an aspect of an embodiment of the present invention, in the soil improvement composition, the bacterial body may be bacteria or fungi.
在本發明的一態樣中,所述之土壤改良組成物,其中該真菌係杏鮑菇菌(Pleurotuseryngii)或秀珍菇菌(Pleurotusostreatus),但並不以此為限。 In one aspect of the present invention, in the soil improvement composition, the fungus is Pleurotus eryngii ( Pleurotuseryngii ) or Pleurotusostreatus , but it is not limited thereto.
在本發明的一實施例中,所述之土壤改良組成物,其中該微藻可為普通小球藻(Chlorella vulgaris)、原始(核)小球藻(Chlorella protothecoides)、蛋白核小球藻(Chlorella pyrenoidosa)、橢圓核小球藻(Chlorella ellipsodiea)、微小小球藻(Chlorella minutissima)或酪蠅小球藻(Chlorella protophila)。 In an embodiment of the present invention, in the soil improvement composition, the microalgae may be Chlorella vulgaris , Chlorella protothecoides , and Chlorella protethecoides ( Chlorella pyrenoidosa ), Chlorella ellipsodiea , Chlorella minutissima or Chlorella protophila .
在本發明的一實施例中,所述之土壤改良組成物,其中該藻類破壁發酵物原液相較於水可占0.5~2%(v/v),而該益生菌混合液相較於水可占1~3%(v/v)。較佳的,該藻類破壁發酵物原液相較於水係約占1%(v/v),而該益生菌混合液相較於水係約占2%(v/v)。 In an embodiment of the present invention, in the soil improvement composition, the original liquid phase of the algae wall-breaking fermentation product can occupy 0.5-2% (v/v) of water, and the probiotic mixed liquid phase Yushui can account for 1~3% (v/v). Preferably, the original liquid phase of the algae wall-breaking fermentation product accounts for about 1% (v/v) of the water system, and the probiotic mixed liquid phase accounts for about 2% (v/v) of the water system.
本發明同時提供一種土壤改良與促進植物生長之方法,包括:於一待改良土地上,灌注如前述之土壤改良組成物。 The invention also provides a method for soil improvement and plant growth promotion, comprising: pouring a soil improvement composition as described above on a land to be improved.
在本發明的一實施例中,所述之土壤改良與促進植物生長之方法,其中該土壤改良組成物係每分地使用800~1200公升,較佳 為900~1100公升,更佳為1000公升。 In an embodiment of the present invention, the method of soil improvement and plant growth promotion, wherein the soil improvement composition is 800-1200 liters per minute, preferably 900 to 1100 liters, more preferably 1000 liters.
藉由本發明土壤改良組成物,一方面使土壤中的微生物相獲得改善,改變了原有酸鹼值、導電度、有機質以及磷、鉀、鈣等有效性成分含量,一方面也藉由該藻類所具有豐富之有機化合物,特別是胺基酸,或是發酵物中其他的有效成分,提供將來耕種作物或植栽最適當的養分以促進其生長。 With the soil improvement composition of the present invention, on the one hand, the microbial phase in the soil is improved, the original pH value, electrical conductivity, organic matter, and the content of effective ingredients such as phosphorus, potassium, and calcium are changed. On the one hand, the algae The rich organic compounds, especially amino acids, or other active ingredients in the ferment, provide the most suitable nutrients for future cultivation of crops or plants to promote their growth.
以下將進一步說明本發明的實施方式,下述所列舉的實施例係用以闡明本發明,並非用以限定本發明之範圍,任何熟習此技藝者,在不脫離本發明之精神和範圍內,當可做些許更動與潤飾,因此本發明之保護範圍當視後附之申請專利範圍所界定者為準。 The embodiments of the present invention will be further described below. The examples listed below are used to clarify the present invention, and are not intended to limit the scope of the present invention. Anyone who is familiar with this art, without departing from the spirit and scope of the present invention, Some changes and retouching can be done, so the scope of protection of the present invention shall be deemed as defined by the scope of the attached patent application.
第1圖係本發明實施例之土壤改良組成物將土壤改良後之土地照片比較。 Figure 1 is a comparison of photos of the soil after the soil improvement of the soil improvement composition of the embodiment of the present invention.
第2圖係本發明實施例之土壤改良組成物將土壤改良後進行一條根施作之照片比較。 Figure 2 is a photographic comparison of the soil improvement composition of the embodiment of the present invention where the roots are applied after soil improvement.
第3圖係本發明實施例之土壤改良組成物將土壤改良後進行高粱施作之照片比較。 Fig. 3 is a photograph comparison of the soil improvement composition of the embodiment of the present invention where sorghum is applied after soil improvement.
第4圖係本發明實施例之土壤改良組成物將土壤改良後進行溫室葉菜類施作之照片比較。 FIG. 4 is a photo comparison of the soil improvement composition of the embodiment of the present invention after the soil improvement is carried out and the greenhouse leafy vegetables are applied.
本發明所提供之土壤改良組成物,首先進行益生菌混合液與藻類破壁發酵物的製備。 The soil improvement composition provided by the present invention firstly prepares the probiotic mixed solution and the algae wall-breaking fermentation product.
分別準備光合菌(Photosynthetic bacteria)、硝化菌(Nitrifyingbacteria) 、枯草桿菌(Bacillus subtilis)、乳酸菌(Lactobacillus)與放線菌(Actinobacteria)等菌液,混合各益生菌液,調配使各該菌體之菌數符合以下表1。惟,益生菌之菌數與比例並非絕對,表1僅為較佳例示,可依土壤目前狀況以及將來所欲耕種之作物調整。本實施例所製備的益生菌混合液包括光合菌、硝化菌、枯草桿菌、乳酸菌與放線菌,總菌數約為8 x 109CFU/mL,而pH值大約5.8。 Were prepared photosynthetic bacteria (Photosynthetic bacteria), nitrifying bacteria (Nitrifyingbacteria), Bacillus subtilis (Bacillus subtilis), lactic acid bacteria (Lactobacillus) and actinomycetes (of Actinobacteria) and other bacteria, probiotics were mixed liquid formulation of each of the bacterial strains The number is in accordance with Table 1 below. However, the number and proportion of probiotics are not absolute. Table 1 is only a good example, which can be adjusted according to the current status of the soil and the crops to be cultivated in the future. The probiotic mixed solution prepared in this example includes photosynthetic bacteria, nitrifying bacteria, Bacillus subtilis, lactic acid bacteria, and actinomycetes. The total bacterial count is about 8 x 10 9 CFU/mL, and the pH value is about 5.8.
藻類破壁發酵物的製備,首先分別準備真菌溶液與藻類溶液。於本實施例中所利用之菌類屬真菌界的杏鮑菇菌(Pleurotuseryngii),將市售的杏鮑菇菌以刀子切開約5mm正方形塊狀接種於固態培養基上(15g/L麥芽精粉、20g/L葡萄糖、6g/L酵母萃取物與15g/L瓊脂),培養約7天。之後將前述瓊脂培養基挖出,加入250ml無菌水進行均質化後備用;關於藻類部分,本實施例則係取0.5g的綠藻粉(Chlorella vulgaris)加入10ml(重量比 約1:20)的無菌水中,攪拌均勻後備用。取前述10ml均質化後的杏鮑菇菌液,加入80ml的液態培養液中(15g/L麥芽精粉、20g/L葡萄糖與6g/L酵母萃取物),再加入10ml綠藻溶液,之後於50L發酵桶中,於室溫下(25~35℃),進行厭氧發酵約3天,即可獲得藻類破壁發酵物原液。若須以乾燥狀態取用,則可將此原液以70℃進行烘乾。 For the preparation of the algae wall-breaking fermentation product, first prepare the fungal solution and the algae solution separately. The Pleurotus eryngii ( Pleurotuseryngii ) belonging to the fungal genus used in this example was inoculated with a commercially available Pleurotus eryngii mushroom with a knife about 5 mm square and inoculated on a solid medium (15 g/L malt extract powder) , 20g/L glucose, 6g/L yeast extract and 15g/L agar), cultured for about 7 days. After that, the aforementioned agar medium was dug out, 250ml of sterile water was added for homogenization, and then used; for the algae part, in this example, 0.5g of green algae powder ( Chlorella vulgaris ) was added to 10ml (weight ratio of about 1:20) aseptic In water, stir well and set aside. Take the aforementioned 10ml homogenized Pleurotus eryngii bacterial solution, add it to 80ml of liquid culture solution (15g/L malt extract powder, 20g/L glucose and 6g/L yeast extract), and then add 10ml of green algae solution. In a 50L fermentation tank, perform anaerobic fermentation at room temperature (25~35℃) for about 3 days to obtain the stock solution of algae wall-breaking fermentation product. If it is necessary to use it in a dry state, it can be dried at 70℃.
經檢測,本發明實施例所製備之藻類破壁發酵物原液中之總氮約為8.9g/mL,水解胺基酸與游離胺基酸分別為3.48g/mL與2.76g/mL,並含有1.46g/mL的綠藻生長因子(CGF)。 After testing, the total nitrogen in the stock solution of algae wall-breaking ferment prepared in the examples of the present invention is about 8.9 g/mL, the hydrolyzed amino acids and free amino acids are 3.48 g/mL and 2.76 g/mL, respectively, and contain 1.46g/mL of green algae growth factor (CGF).
前述藻類破壁發酵物的製備,係利用菌體所分泌的纖維素水解酵素對藻類細胞壁進行水解,相較於研磨或切割的機械方式,或是利用反覆冷凍、超音波震盪的物理方法、還是利用特殊酵素分解、有機溶劑滲透的化學方法,利用本發明生物自然分泌的纖維素水解酵素,更能夠在高效率分解細胞壁的同時,較為完整並保留該藻類內含的所有成分,而可充分利用其內容物所帶來的功效。 The preparation of the algae wall-breaking fermentation product is to use the cellulolytic enzyme secreted by the bacteria to hydrolyze the cell wall of the algae. Compared with the mechanical method of grinding or cutting, or the physical method of using repeated freezing and ultrasonic vibration, or Using the chemical method of special enzyme decomposition and organic solvent penetration, the cellulose hydrolyzing enzyme naturally secreted by the organism of the present invention can decompose the cell wall with high efficiency, and it is more complete and retains all the components contained in the algae, which can be fully utilized The effectiveness of its contents.
前述所利用的綠藻粉中之綠藻又稱小球藻、綠球藻,係一種微藻,生長在新鮮、無污染的水域,屬單細胞植物,細胞大小約只有2~8μm。綠藻具有植物的兩大特徵:具有葉綠體及細胞壁,其葉綠體中含有葉綠素a及b、葉紅素、葉黃素等,而其光合作用的方式和高等植物相同。綠藻在分類學上屬植物界(Kingdom Plantae)、綠藻門(Division Chlorophyta)、綠球藻綱(ClassChlorophyceae)、綠球藻目(OrderChlorocaccales)、綠球藻科(Family Chlorellaceae)的綠球藻屬(GeneraChlorella)。可利用的綠球藻除前述普通小球藻(Chlorellavulgaris)外,尚包括:原始(核)小球藻(Chlorella rotothecoides)、蛋白核小球藻(Chlorella pyrenoidosa)、橢圓核小球藻(Chlorella ellipsodiea)、微小小球藻(Chlorella minutissima)或酪蠅小球藻(Chlorella protophila)等,但並不僅限於此。 The green algae in the aforementioned green algae powder, also known as chlorella, chlorella, is a kind of microalgae, which grows in fresh, non-polluted waters and belongs to a single-cell plant. The cell size is only about 2~8μm. Chlorella has two major characteristics of plants: it has chloroplasts and cell walls. Its chloroplasts contain chlorophyll a and b, erythrophyll, lutein, etc., and its photosynthesis is the same as higher plants. Chlorophytes are taxonomically classified as Chlorophyceae of the Kingdom Plantae, Division Chlorophyta, Class Chlorophyceae, Order Chlorocaccales, Family Chlorellaceae (GeneraChlorella). Available Chlorella in addition to the aforementioned common Chlorella ( Chlorellavulgaris ), also includes: original (nuclear) Chlorella ( Chlorella rotothecoides ), Chlorella pyrenoidosa ( Chlorella pyrenoidosa ), Chlorella ellipsodiea ( Chlorella ellipsodiea) ), Chlorella minutissima , Chlorella protophila , etc., but not limited to this.
前述纖維素水解酵素,可將不具溶解性的纖維素分解成單糖。該些酵素可包括但不限於:內切型纖維素纖維分解酵素(endo-β-1,4-glucanase)E.C.3.2.1.4(又稱β-1,4-D-glucanhydrolase或carboxymethylcellulase(CMCase))、外切型纖維素纖維分解酵素(exo-β-1,4-glucanase)E.C.3.2.1.91(又稱β-1,4-glucancellobiohydrolase或cellulose-β-1,4-glucancellobiosidase)、β-葡萄糖甘酵素(β-1,4-glucosidase)E.C.3.2.1.21(又稱β-1,4-glucohydrolase或cellobiase)。 The aforementioned cellulose hydrolyzing enzyme can decompose insoluble cellulose into monosaccharides. These enzymes may include, but are not limited to: endo-β-1,4-glucanase EC3.2.1.4 (also known as β-1,4-D-glucanhydrolase or carboxymethylcellulase (CMCase)) 、Exo-β-1,4-glucanase EC3.2.1.91 (also known as β-1,4-glucancellobiohydrolase or cellulose-β-1,4-glucancellobiosidase), β-glucan Enzyme (β-1,4-glucosidase) EC3.2.1.21 (also known as β-1,4-glucohydrolase or cellobiase).
前述纖維素水解酵素可由不同的菌體所分泌,例如秀珍菇菌(Pleurotusostreatus)可分泌水解羧甲基纖維素(Carboxymethylcellulose,CMC)。 The aforementioned cellulose hydrolyzing enzymes can be secreted by different bacteria, for example, Pleurotusostreatus can secrete hydrolyzed carboxymethyl cellulose (Carboxymethylcellulose, CMC).
前述杏鮑菇菌僅為例示,其亦可為真菌界的秀珍菇菌(Pleurotusostreatus),或其他可以分泌前述將藻類細胞壁加以水解的酵素的菌株。 The above-mentioned Pleurotus eryngii is only an example, and it may also be Pleurotusostreatus in the fungal world, or other strains that can secrete the enzyme that hydrolyzes the algal cell wall.
前述微藻溶液與菌體溶液的比例並未設有特別的限制,可在0.5:1~1:5的範圍內,較佳為1:1~1:3之間,更佳為1:1。 The ratio of the aforementioned microalgae solution to the bacterial cell solution is not particularly limited, and may be in the range of 0.5:1 to 1:5, preferably 1:1 to 1:3, more preferably 1:1 .
本發明之土壤改良組成物,主要係將藻類破壁發酵物原液、益生菌混合液與水進行一預定比例之混合。所使用之水並無特別的限制,可為一般灌溉用水、地下水或井水。藻類破壁發酵物原液、益生菌混合液與水的比例分別約為0.5~2%(v/v)與1~3%(v/v)。較佳的,藻類破壁發酵物原液、益生菌混合液與水的比例分別約為1%(v/v)與2%(v/v)。 The soil improvement composition of the present invention mainly consists of mixing the algae wall-breaking fermentation product stock solution, probiotic mixed solution and water at a predetermined ratio. The water used is not particularly limited, and can be general irrigation water, ground water or well water. The ratio of the algae wall-broken fermentation raw material liquid, probiotic mixed liquid and water is about 0.5~2% (v/v) and 1~3% (v/v), respectively. Preferably, the ratio of the algae wall-breaking fermentation product stock solution, the probiotic mixed solution and water is about 1% (v/v) and 2% (v/v), respectively.
選取二塊相近但相隔約100公尺,地質相同、地像相近約1000平方 公尺面積之土地,分別作為實驗組與對照組。首先檢測實驗組與對照組土地之酸鹼值、導電度、有機質以及以下表2所列有效性成分。檢測時可將各塊土地畫作九宮格,而分別至少於四角落與中央區域取樣檢測。取樣時,分為以表土(0~15公分)與底土(15~30公分)採取土壤樣本。檢測週期為實驗前進行第1次檢測,而後分別於實驗後1、2、3個月時各檢測一次,故共檢測四次。 Select two blocks close to each other but about 100 meters apart, with the same geology and similar ground images about 1000 square meters The land with a meter area is used as the experimental group and the control group, respectively. First, test the pH value, electrical conductivity, organic matter and the effective ingredients listed in Table 2 below. During the inspection, each piece of land can be painted as a nine-square grid, and samples should be sampled at least in the four corners and the central area. When sampling, it is divided into taking top soil (0~15 cm) and subsoil (15~30 cm) to take soil samples. The detection period is the first test before the experiment, and then one test at 1, 2, and 3 months after the experiment, so a total of four tests are performed.
施作時,取970公升的水體,加上20公升於實施例1中所製備的益生菌混合液,以及10公升於實施例2中所製備的藻類破壁發酵物原液,均勻混合後,即可平均灌注於實驗組之土地上,對照組則灌以1000公升的水體。灌注時,每7天灌注1次,持續灌注3個月,共12次。3個月後,最終檢測之結果如表2~表4與圖1所示。 During application, take 970 liters of water, add 20 liters of the probiotic mixed solution prepared in Example 1, and 10 liters of the algae-walled fermentation broth prepared in Example 2, after uniformly mixing, that is It can be poured on the land of the experimental group on average, and the control group is filled with 1000 liters of water. During perfusion, perfusion was performed every 7 days and continued for 3 months, a total of 12 times. After 3 months, the final test results are shown in Table 2~Table 4 and Figure 1.
由表2~表4與第1圖之結果可知,土壤改良前土地結塊的現象獲得改善。就酸鹼值部分,使原本偏鹼的土質往中性降低,約略在pH 6.2~6.8,而導電度(ec)則明顯由約0.37~0.45降低至0.24~0.28,可知過多金屬離子部分已被代謝後降低。至於有機質(om)部分,由於本發明土壤組成分中包括藻類破壁發酵物原液,透過益生菌的作用或利用,大幅由原本的0.21~0.32增加至5.31~7.6,為土壤提供了豐富的基礎養分。此外,在調整植物生長必須元素部分,將原本過高的磷含量降低至43~59,將鉀降低至57~79,將鎂降至74~63,而鈣則增加至830~956。其他重金屬部分,則調整至一般之標準值的範圍內。由此實驗結果可知,藉由本發明土壤改良組成物,的確具有顯著土壤改質的效果,並同時預先補充了該基地植物生長所需的養分。 From the results of Table 2 to Table 4 and Figure 1, it can be seen that the phenomenon of land agglomeration before soil improvement is improved. As for the pH value part, the originally alkaline soil is reduced to neutrality, about pH 6.2~6.8, and the electrical conductivity (ec) is obviously reduced from about 0.37~0.45 to 0.24~0.28. Reduced after metabolism. As for the organic matter (om) part, since the soil component of the present invention includes the raw liquid of algae wall-breaking fermented material, through the action or utilization of probiotics, it is greatly increased from the original 0.21~0.32 to 5.31~7.6, providing a rich foundation for the soil nutrient. In addition, in the adjustment of essential elements for plant growth, the originally excessive phosphorus content was reduced to 43-59, potassium to 57-79, magnesium to 74-63, and calcium to 830-956. Other heavy metal parts are adjusted to the range of general standard values. From the experimental results, it can be known that the soil improvement composition of the present invention does have a significant effect of soil modification, and at the same time supplements the nutrients required for the growth of the base plant in advance.
取1000株品種為闊葉大豆(GlyinetomentellaHayata.)的一條根,分別於50平方公尺(播種2行,株距10公分)進行實驗組(經土壤改良)與對照組之試驗。土壤改良施作時,取970公升的水體,加上20公升於實施例1中所製備的益生菌混合液,以及10公升於實施例2中所製備的藻類破壁發酵物原液,均勻混 合後,即可平均灌注於實驗組之土地上,對照組則灌以1000公升的水體。灌注時,每7天灌注1次,持續灌注3個月,共12次。3個月後觀察最終土壤檢測結果及種植一條根後其植株生長狀況,結果如表5~表7與圖2所示。 1000 roots of broad-leaf soybean (Glyinetomentella Hayata.) were taken and tested at 50 square meters (sown 2 rows, plant spacing 10 cm) in the experimental group (with soil improvement) and the control group. For soil improvement, take 970 liters of water, add 20 liters of the probiotic mixture prepared in Example 1, and 10 liters of the algae-walled fermentation broth prepared in Example 2 and mix them evenly. After closing, it can be poured on the land of the experimental group on average, and the control group is filled with 1000 liters of water. During perfusion, perfusion was performed every 7 days and continued for 3 months, a total of 12 times. After 3 months, observe the final soil test results and the plant growth status after planting a root. The results are shown in Table 5~Table 7 and Figure 2.
由表5~表7與第2圖之結果可知,未經土壤改良的土地,土壤肥力極度不足,植株生長情形惡劣,葉子極度短小。但相較之下,經土壤改良後,土壤肥力均勻,葉子生長茂盛,植株生長狀況良好。一條根1週芽點數對照組僅 有5.00%,經土壤改良後之實驗組植株,大幅增加至85.00%。植株生長率由原本的8.75%增加至63.25%,提高其生長速率約54.50%。由此實驗結果可知,藉由本發明土壤改良組成物,的確具有顯著土壤改良的效果,並同時預先補充了該基地植物生長所需的養分。 From the results of Table 5 to Table 7 and Figure 2, it can be seen that the soil without soil improvement has extremely poor soil fertility, poor plant growth and extremely short leaves. But by comparison, after soil improvement, the soil fertility is uniform, the leaves grow lush, and the plants grow well. One root one week bud points control group only There were 5.00% of the plants in the experimental group after soil improvement, which increased to 85.00%. The plant growth rate increased from 8.75% to 63.25%, increasing its growth rate by about 54.50%. From the experimental results, it can be known that the soil improvement composition of the present invention does have a significant soil improvement effect, and at the same time supplements the nutrients required for the growth of the base plant in advance.
取1000株品種為豐糯四號的高粱,分別於50平方公尺(播種2行,株距10公分)進行實驗組(經土壤改良)與對照組之試驗。土壤改良施作時,取970公升的水體,加上20公升於實施例1中所製備的益生菌混合液,以及10公升於實施例2中所製備的藻類破壁發酵物原液,均勻混合後,即可平均灌注於實驗組之土地上,對照組則灌以1000公升的水體。灌注時,每7天灌注1次,持續灌注3個月,共12次。3個月後觀察最終土壤檢測結果及種植高粱後其植株生長狀況,其結果如表8~表10與圖3所示。 Take 1000 sorghum cultivars of Fengnuo No. 4 and test them in the experimental group (improved soil) and the control group at 50 square meters (2 rows of planting, plant spacing 10 cm). For soil improvement, take 970 liters of water, add 20 liters of the probiotic mixed solution prepared in Example 1, and 10 liters of the algae-walled fermented stock solution prepared in Example 2, and mix them evenly. , It can be poured on the land of the experimental group on average, and the control group is filled with 1000 liters of water. During perfusion, perfusion was performed every 7 days and continued for 3 months, a total of 12 times. After 3 months, observe the final soil test results and the plant growth status after planting sorghum. The results are shown in Table 8~Table 10 and Figure 3.
由表8~表10與第3圖之結果可知,未經土壤改良的土地,土壤肥力明顯不足且營養不均,使作物用地無法有效利用,導致植株生長不佳。但相較之下,經土壤改良後,土壤肥力平均健康,高粱植株的生長狀況良好。高粱實驗組中,每一植株的體型要比未經土壤改良之對照組壯大許多,植株長增加了10公分,其開花生長速率增加了約41.50%,使整體的產量大幅提高。由此實驗結果可知,藉由本發明土壤改良組成物,的確具有顯著土壤改良的效果,並同時預先補充了該基地植物生長所需的養分。 From the results of Table 8 to Table 10 and Figure 3, it can be seen that the land without soil improvement has obviously insufficient soil fertility and uneven nutrition, which makes the crop land unable to be effectively used, resulting in poor plant growth. However, by comparison, after soil improvement, the soil fertility was average and healthy, and the sorghum plants grew well. In the sorghum experimental group, the size of each plant is much larger than that of the control group without soil improvement. The plant length is increased by 10 cm, and the flowering growth rate is increased by about 41.50%, which greatly increases the overall yield. From the experimental results, it can be known that the soil improvement composition of the present invention does have a significant soil improvement effect, and at the same time supplements the nutrients required for the growth of the base plant in advance.
取1000株小白菜,分別於50平方公尺(播種2行,株距10公分)進行實驗組(經土壤改良)與對照組之試驗。土壤改良施作時,取970公升的水體,加上20公升於實施例1中所製備的益生菌混合液,以及10公升於實施例2中所製備的藻類破壁發酵物原液,均勻混合後,即可平均灌注於實驗組之土地上,對照組則灌以1000公升的水體。灌注時,每7天灌注1次,持續灌注3個月,共12次。3個月後觀察最終土壤檢測結果及種植小白菜後其植株生長狀況,結果如表11~表13與圖4所示。 1000 plants of Chinese cabbage were taken and tested in the experimental group (with soil improvement) and the control group at 50 square meters (sown 2 rows, plant spacing 10 cm). For soil improvement, take 970 liters of water, add 20 liters of the probiotic mixed solution prepared in Example 1, and 10 liters of the algae-walled fermented stock solution prepared in Example 2, and mix them evenly. , It can be poured on the land of the experimental group on average, and the control group is filled with 1000 liters of water. During perfusion, perfusion was performed every 7 days and continued for 3 months, a total of 12 times. After 3 months, observe the final soil test results and the plant growth after planting Chinese cabbage. The results are shown in Table 11 to Table 13 and Figure 4.
由表11~表13與第4圖之結果可知,未經土壤改良的土地,土壤肥力明顯不足且營養不均,使作物用地無法有效利用,導致植株生長不佳,根系的擴展有限。但相較之下,經土壤改良後,土壤肥力平均健康,小白菜植株的生長狀況良好。小白菜實驗組中,無論葉長、葉寬、莖長、莖粗都增加了約25%~80%,且葉片數目也增加了83%,使整體的產量大幅提高。此外,觀察菜株上之蛀蟲,也發現實驗組的部分減少了7%,因此,由此實驗結果可知,藉由 本發明土壤改良組成物,的確具有顯著土壤改質的效果,並同時預先補充了該基地植物生長所需的養分及提高了植株對蛀蟲的抵抗力。 From the results of Table 11 to Table 13 and Figure 4, it can be seen that the land without soil improvement has obviously insufficient soil fertility and uneven nutrition, which makes the crop land unable to be effectively used, resulting in poor plant growth and limited root expansion. However, by comparison, after soil improvement, the soil fertility was average and healthy, and the growth of Chinese cabbage plants was good. In the Chinese cabbage experiment group, no matter the leaf length, leaf width, stem length, and stem thickness increased by about 25% to 80%, and the number of leaves also increased by 83%, the overall yield was greatly improved. In addition, observing the moth on the vegetable plant also found that the part of the experimental group was reduced by 7%. Therefore, from the experimental results, we can see that by The soil improvement composition of the present invention does have a significant effect of soil modification, and at the same time supplements in advance the nutrients required for the growth of the base plant and improves the plant's resistance to borers.
藉由上述試驗可知,本發明土壤改良組成物,除了可改善並調整土壤之成分與特性外,亦可藉由該藻類破壁發酵物所含有之其他有效成分,提供植株生長所需之養分或元素,因此能進一步促進植物之發育與成長,是一種較以往有限效果的單純土壤改良,具有更優秀土壤改質效果且更能促進植物生長之方法。 From the above test, it can be seen that the soil improvement composition of the present invention can not only improve and adjust the composition and characteristics of the soil, but also provide other nutrients required by the plant growth through other effective ingredients contained in the algae wall-breaking fermentation product or Elements, therefore, can further promote the development and growth of plants. It is a simple soil improvement with limited effects over the past. It has a better soil modification effect and can promote plant growth.
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