CN110226504A - A kind of cultural method promoting Resistance of Wheat To Adversity - Google Patents

A kind of cultural method promoting Resistance of Wheat To Adversity Download PDF

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Publication number
CN110226504A
CN110226504A CN201910628776.0A CN201910628776A CN110226504A CN 110226504 A CN110226504 A CN 110226504A CN 201910628776 A CN201910628776 A CN 201910628776A CN 110226504 A CN110226504 A CN 110226504A
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wheat
liquid
adversity
cultural method
seed
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CN110226504B (en
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雷梦林
刘霞
穆志新
王宇楠
黄蕊
曹利萍
张晋
康国帅
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INSTITUTE OF CROP GERMPLASM RESOURCES SHANXI ACADEMY OF AGRICULTURAL SCIENCES
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INSTITUTE OF CROP GERMPLASM RESOURCES SHANXI ACADEMY OF AGRICULTURAL SCIENCES
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G31/00—Soilless cultivation, e.g. hydroponics
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/36—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids
    • A01N37/38—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids having at least one oxygen or sulfur atom attached to an aromatic ring system
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/36—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids
    • A01N37/38—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids having at least one oxygen or sulfur atom attached to an aromatic ring system
    • A01N37/40—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids having at least one oxygen or sulfur atom attached to an aromatic ring system having at least one carboxylic group or a thio analogue, or a derivative thereof, and one oxygen or sulfur atom attached to the same aromatic ring system
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F11/00—Other organic fertilisers
    • C05F11/10—Fertilisers containing plant vitamins or hormones
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G5/00—Fertilisers characterised by their form
    • C05G5/20—Liquid fertilisers

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  • Life Sciences & Earth Sciences (AREA)
  • Pest Control & Pesticides (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Environmental Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Agronomy & Crop Science (AREA)
  • Plant Pathology (AREA)
  • Engineering & Computer Science (AREA)
  • Dentistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Botany (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Cultivation Of Plants (AREA)

Abstract

The invention discloses a kind of cultural methods for promoting Resistance of Wheat To Adversity.Method includes the following steps: wheat seed is placed in the concentrated sulfuric acid and soaks seed by (1), disinfection is taken out, is dried after cleaning；It (2) will treated that wheat seed is placed in adjusts in liquid and impregnate through step (1)；(3) seed obtained by step (2) is placed in nutrient solution and is cultivated to its germination；(4) it by the wheat transplantation of seedlings after germination into Nutrition Soil, is cultivated according to a conventional method to its maturation.The wheat seedling that the method for the present invention is cultivated, survival rate is high, and has excellent resistance.

Description

A kind of cultural method promoting Resistance of Wheat To Adversity
Technical field
The invention belongs to wheat Cultivating techniques fields, and in particular to a kind of cultural method for promoting Resistance of Wheat To Adversity.
Background technique
Wheat is one of three big cereal, is a kind of staple food crop widely planted in the world, yield almost full conduct It is edible.Wheat is the general designation of wheat system plant, is monocotyledon, is a kind of grass family plant planted extensively all over the world Object, the caryopsis of wheat are one of staple foods of the mankind, can make the foods such as bread, steamed bun, biscuit, noodles after wearing into flour；Fermentation After can be made into beer, alcohol, white wine (such as vodka), or raw matter fuel.Wheat rich in starch, protein, fat, minerals, Calcium, iron, thiamine, riboflavin, niacin, vitamin A and vitamin C etc..China is large agricultural country, the plantation amount and plantation of wheat Region is all very big.Wheat seed production all multisystems and link in, Seed-breeding techniques be to ensure that wheat seed quality and Yield reaches the important link of requirement.But the resistance such as the wheat insect pest that existing method is cultivated are poor.
Summary of the invention
For above-mentioned deficiency in the prior art, the present invention provides a kind of cultural method for promoting Resistance of Wheat To Adversity, can have Effect solves the problems, such as the resistance such as existing wheat pest-resistant in growth and development process, cold-resistant deficiency.
To achieve the above object, the technical solution adopted by the present invention to solve the technical problems is:
A kind of cultural method promoting Resistance of Wheat To Adversity, which comprises the following steps:
(1) wheat seed is placed in 5~10min of seed soaking in the concentrated sulfuric acid, takes out disinfection, dried after cleaning；
(2) will through step (1), treated that wheat seed is placed in adjusts in liquid, continue 20~30h of immersion in 24~30 DEG C； Adjusting liquid includes glycine betaine, 2,6 dichloro-isonicotinic acids, 6,7- dihydroxycoumarin and zasiokaurin liquid；Wherein, glycine betaine, 2,6 The weight ratio of dichloro-isonicotinic acid, 6,7- dihydroxycoumarin and zasiokaurin liquid is 0.2~1:0.5~1.5:0.05~0.2: 0.5~1；
(3) seed obtained by step (2) is placed in nutrient solution, is cultivated in 4~10 DEG C, 1100~1400Lux to its germination；
(4) the wheat transplantation of seedlings after germination is continued under conditions of -1~1 DEG C, 1300~1500Lux into Nutrition Soil After cultivating 5~10 days, cultivated according to a conventional method to its maturation.
Further, glycine betaine, 2,6 dichloro-isonicotinic acids, Esculetin and zasiokaurin liquid in step (1) Weight ratio be 0.2:0.5:0.08:1.
Further, liquid is adjusted in step (1) further includes the mixed liquor for accounting for its weight 8~15%；Mixed liquor includes weight Than for 0.5~1:0.5~1:1~1.5 ferulic acid, gallic acid and chlorogenic acid.
Further, in step (2) zasiokaurin liquid the preparation method comprises the following steps:
After -20~-10 DEG C of 5~10h of fresh corn pollen, places it in 50~80 DEG C of water and stir evenly, it is cooling To room temperature.
Further, nutrient solution includes plant nutrition liquid, abscisic acid and methyl mercapto isopentenyl adenosine in step (3)； Wherein, the volume ratio of plant nutrition liquid, abscisic acid and methyl mercapto isopentenyl adenosine be 1.2~1.5:0.4~0.5:0.5~ 0.6。
Further, the volume ratio of plant nutrition liquid, abscisic acid and methyl mercapto isopentenyl adenosine is 1.2:0.5: 0.5。
Further, plant nutrition liquid is wheat plant nutrient solution.
Further, condition of culture in step (3) are as follows: 4 DEG C, 1200Lux.
Further, Nutrition Soil includes humus, vermiculite, ammonium sulfate, megasse and lactobacillus-fermented in step (4) Object；Wherein, the weight ratio of humus, vermiculite, ammonium sulfate, megasse and lactobacillus fermentation is 1.2~1.5:1~1.5: 0.1~0.5:1~1.5:0.1~0.5.
Further, the weight ratio of humus, vermiculite, ammonium sulfate, megasse and lactobacillus fermentation is 1.5:1: 0.3:1.2:0.1。
The invention has the benefit that
1, there are innate defence network in plant, plant can effectively be promoted to pest and disease damage, and in various adverse circumstances Resistance in environment, garden beet alkali, 2,6 dichloro-isonicotinic acids, Esculetin and zasiokaurin liquid of the present invention are matched Set adjusting liquid, can innate defence network in effective activated plant body, to achieve the purpose that promote plant resistance；Its In, 2,6 dichloro-isonicotinic acids and glycine betaine are as the main component for activating its defending against network, with Esculetin and corn Pollen liquid carries out compatibility as modifying ingredients and 2,6 dichloro-isonicotinic acids and glycine betaine, maximizes so that its effect has, and simultaneously will Its side effect is down in tolerance interval.
2, it adjusts in liquid added with the mixed liquor being made of ferulic acid, gallic acid and chlorogenic acid, can further mention The content for rising the enzymes such as peroxidase, superoxide dismutase in wheat, can effectively promote the vigor of seed, further to promote Into the promotion of Resistance of Wheat To Adversity.
3, in the nutrient solution in the present invention, plant nutrition liquid provides nutrition, abscisic acid and first as nutritional ingredient for wheat The compounding of sulfenyl isopentenyl adenosine can effectively eliminate the counter productive using single medicament, wherein abscisic acid effectively promotes Plant root growth, and overcome methyl mercapto isopentenyl adenosine and make the defect of plant height excessively rapid growth, meanwhile, abscisic acid is to kind The inhibition of son germination is effectively cleared up by methyl mercapto isopentenyl adenosine, can significantly enhance the resistance of wheat, have Extremely significant synergistic effect.
Specific embodiment
A specific embodiment of the invention is described below, in order to facilitate understanding by those skilled in the art this hair It is bright, it should be apparent that the present invention is not limited to the ranges of specific embodiment, for those skilled in the art, As long as various change is in the spirit and scope of the present invention that the attached claims limit and determine, these variations are aobvious and easy See, all are using the innovation and creation of present inventive concept in the column of protection.
Embodiment 1
A kind of cultural method promoting Resistance of Wheat To Adversity, comprising the following steps:
(1) wheat seed is placed in the concentrated sulfuric acid 10min that soaks seed, taken out, successively with after ethyl alcohol and hypochlorite disinfectant, washed Only it dries；
(2) will through step (1), treated that wheat seed is placed in adjusts in liquid, continue to impregnate 22h in 24 DEG C；Wherein, it adjusts Section liquid includes glycine betaine, 2,6 dichloro-isonicotinic acids, 6,7- dihydroxycoumarin and the corn that weight ratio is 0.2:0.5:0.08:1 Pollen liquid；Wheat seed and the weight ratio for adjusting liquid are 1:3；
Also, also added with the mixed liquor for accounting for its weight 8% in adjusting liquid；The mixed liquor includes that weight ratio is 1:1:1 Ferulic acid, gallic acid and chlorogenic acid；
The zasiokaurin liquid the preparation method is as follows:
Zasiokaurin is acquired, and places it in the environment that temperature is -20 DEG C and freezes 8h, then takes out and is 60 with temperature DEG C hot water mixing, stir evenly；
(3) seed obtained by step (2) is placed in nutrient solution, under conditions of 4 DEG C, 1200Lux, is cultivated to its germination； Wherein, nutrient solution includes wheat plant nutrient solution, abscisic acid and the methyl mercapto isopentenyl gland that volume ratio is 1.2:0.5:0.5 Glycosides；
(4) the wheat transplantation of seedlings after germination is continued into cultivation 5~10 into Nutrition Soil under conditions of 1 DEG C, 1300Lux It after it, is cultivated according to a conventional method to its maturation, wherein Nutrition Soil includes the corruption that weight ratio is 1.5:1:0.3:1.2:0.1 Grow matter, vermiculite, ammonium sulfate, megasse and lactobacillus fermentation.
Embodiment 2
A kind of cultural method promoting Resistance of Wheat To Adversity, comprising the following steps:
(1) wheat seed is placed in the concentrated sulfuric acid 5min that soaks seed, taken out, successively with after ethyl alcohol and hypochlorite disinfectant, washed Only it dries；
(2) will through step (1), treated that wheat seed is placed in adjusts in liquid, continue to impregnate for 24 hours in 30 DEG C；Wherein, it adjusts Section liquid includes glycine betaine, 2,6 dichloro-isonicotinic acids, 6,7- dihydroxycoumarin and the corn that weight ratio is 1:1.5:0.05:0.8 Pollen liquid；Wheat seed and the weight ratio for adjusting liquid are 1:3；
The zasiokaurin liquid the preparation method is as follows:
Zasiokaurin is acquired, and places it in the environment that temperature is -15 DEG C and freezes 5h, then takes out and is 60 with temperature DEG C hot water mixing, stir evenly；
(3) seed obtained by step (2) is placed in nutrient solution, under conditions of 8 DEG C, 1400Lux, is cultivated to its germination； Wherein, nutrient solution includes wheat plant nutrient solution, abscisic acid and the methyl mercapto isopentenyl gland that volume ratio is 1.5:0.4:0.6 Glycosides；
(4) by the wheat transplantation of seedlings after germination into Nutrition Soil, continue to cultivate 10 days under conditions of 1 DEG C, 1300Lux Afterwards, it cultivates according to a conventional method to its maturation, wherein Nutrition Soil includes the humic that weight ratio is 1.2:1:0.5:1.3:0.2 Matter, vermiculite, ammonium sulfate, megasse and lactobacillus fermentation.
Embodiment 3
A kind of cultural method promoting Resistance of Wheat To Adversity, comprising the following steps:
(1) wheat seed is placed in the concentrated sulfuric acid 10min that soaks seed, taken out, successively with after ethyl alcohol and hypochlorite disinfectant, washed Only it dries；
(2) will through step (1), treated that wheat seed is placed in adjusts in liquid, continue to impregnate 20h in 30 DEG C；Wherein, it adjusts Section liquid includes glycine betaine, 2,6 dichloro-isonicotinic acids, 6,7- dihydroxycoumarin and the corn that weight ratio is 0.4:0.7:0.2:1 Pollen liquid；Wheat seed and the weight ratio for adjusting liquid are 1:3；
Also, also added with the mixed liquor for accounting for its weight 10% in adjusting liquid；The mixed liquor includes that weight ratio is 0.5: Ferulic acid, gallic acid and the chlorogenic acid of 0.5:1.2；
The zasiokaurin liquid the preparation method is as follows:
Zasiokaurin is acquired, and places it in the environment that temperature is -20 DEG C and freezes 8h, then takes out and is 60 with temperature DEG C hot water mixing, stir evenly；
(3) seed obtained by step (2) is placed in nutrient solution, under conditions of 4 DEG C, 1200Lux, is cultivated to its germination； Wherein, nutrient solution includes wheat plant nutrient solution, abscisic acid and the methyl mercapto isopentenyl gland that volume ratio is 1.5:0.4:0.5 Glycosides；
(4) by the wheat transplantation of seedlings after germination into Nutrition Soil, continue to cultivate 10 days under conditions of 1 DEG C, 1300Lux Afterwards, it cultivates according to a conventional method to its maturation, wherein Nutrition Soil includes the humic that weight ratio is 1.2:1.5:0.1:1:0.3 Matter, vermiculite, ammonium sulfate, megasse and lactobacillus fermentation.
Embodiment 4
Compared with Example 1, lacking adjusting liquid in step (1), nutrient solution only includes plant nutrition liquid and abscisic acid, remaining Process is same as Example 1.
Embodiment 5
Compared with Example 1, the nutrient solution in step (3) only includes plant nutrition liquid and methyl mercapto isopentenyl adenosine, Remaining process is same as Example 1.
Embodiment 6
Compared with Example 1, the adjusting liquid in step (1) only includes ferulic acid, gallic acid and chlorogenic acid, remaining process It is same as Example 1.
Embodiment 7
300 pieces of seeds are taken, are cultivated 30~40 days according to Examples 1 to 6 the method respectively, and detect the hair of wheat seed The index parameters such as bud rate, survival rate, plant height, the result is shown in tables 1.
1 wheat Indexs measure of table
From the data in table 1, it can be seen that the indexs such as the germination percentage for the wheat that Examples 1 to 3 is cultivated, survival rate, plant height are excellent In embodiment 4~6, thus illustrate, only under the cooperation of the method for the present invention and reagent, it is strong that obtained resistance could be cultivated Seedling.

Claims (9)

1. a kind of cultural method for promoting Resistance of Wheat To Adversity, which comprises the following steps:
(1) wheat seed is placed in 5~10min of seed soaking in the concentrated sulfuric acid, takes out disinfection, dried after cleaning；
(2) will through step (1), treated that wheat seed is placed in adjusts in liquid, continue 20~30h of immersion in 24~30 DEG C；It is described Adjusting liquid includes glycine betaine, 2,6 dichloro-isonicotinic acids, 6,7- dihydroxycoumarin and zasiokaurin liquid；Wherein, glycine betaine, 2,6 The weight ratio of dichloro-isonicotinic acid, 6,7- dihydroxycoumarin and zasiokaurin liquid is 0.2~1:0.5~1.5:0.05~0.2: 0.5~1；
(3) seed obtained by step (2) is placed in nutrient solution, is cultivated in 4~10 DEG C, 1100~1400Lux to its germination；
(4) the wheat transplantation of seedlings after germination is continued to cultivate into Nutrition Soil under conditions of -1~1 DEG C, 1300~1500Lux After 5~10 days, cultivated according to a conventional method to its maturation.
2. the cultural method according to claim 1 for promoting Resistance of Wheat To Adversity, which is characterized in that sweet tea described in step (1) Dish alkali, 2,6 dichloro-isonicotinic acids, 6,7- dihydroxycoumarin and zasiokaurin liquid weight ratio be 0.2:0.5:0.08:1.
3. the cultural method according to claim 1 for promoting Resistance of Wheat To Adversity, which is characterized in that adjusted described in step (1) Saving liquid further includes the mixed liquor for accounting for its weight 8~15%；The mixed liquor includes that weight ratio is 0.5~1:0.5~1:1~1.5 Ferulic acid, gallic acid and chlorogenic acid.
4. the cultural method according to claim 1 for promoting Resistance of Wheat To Adversity, which is characterized in that jade described in step (2) Rice pollen liquid the preparation method comprises the following steps:
After -20~-10 DEG C of 5~10h of fresh corn pollen, places it in 50~80 DEG C of water and stir evenly, be cooled to room Temperature.
5. the cultural method according to claim 1 for promoting Resistance of Wheat To Adversity, which is characterized in that sought described in step (3) Nutrient solution includes plant nutrition liquid, abscisic acid and methyl mercapto isopentenyl adenosine；Wherein, plant nutrition liquid, abscisic acid and first The volume ratio of sulfenyl isopentenyl adenosine is 1.2~1.5:0.4~0.5:0.5~0.6.
6. the cultural method according to claim 1 for promoting Resistance of Wheat To Adversity, which is characterized in that the plant nutrition liquid, The volume ratio of abscisic acid and methyl mercapto isopentenyl adenosine is 1.2:0.5:0.5.
7. the cultural method according to claim 1 for promoting Resistance of Wheat To Adversity, which is characterized in that trained described in step (3) The condition of supporting are as follows: 4 DEG C, 1200Lux.
8. the cultural method according to claim 1 for promoting Resistance of Wheat To Adversity, which is characterized in that sought described in step (4) Supporting soil includes humus, vermiculite, ammonium sulfate, megasse and lactobacillus fermentation；Wherein, humus, vermiculite, ammonium sulfate, sweet tea Dish slag and the weight ratio of lactobacillus fermentation are 1.2~1.5:1~1.5:0.1~0.5:1~1.5:0.1~0.5.
9. it is according to claim 8 promoted Resistance of Wheat To Adversity cultural method, which is characterized in that the humus, vermiculite, The weight ratio of ammonium sulfate, megasse and lactobacillus fermentation is 1.5:1:0.3:1.2:0.1.
CN201910628776.0A 2019-07-12 2019-07-12 Cultivation method for improving stress resistance of wheat Expired - Fee Related CN110226504B (en)

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CN111226773A (en) * 2020-03-25 2020-06-05 山西省农业科学院小麦研究所 Cultivation method for improving stress resistance of wheat
CN112493068A (en) * 2020-11-04 2021-03-16 山东省农业科学院玉米研究所（山东省农业科学院玉米工程技术研究中心） Corn planting method for realizing weight-reducing and efficiency-increasing effects
CN114158419A (en) * 2021-12-07 2022-03-11 浙江水利水电学院 Method for preventing and controlling water and soil loss

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