TW201825441A

TW201825441A - Use of algae broken-wall fermentate as foliar fertilizer and enhancing leaf crops against insects capable of being applied to crops or plants to promote the growth of stems, leaves and/or fruits thereof

Info

Publication number: TW201825441A
Application number: TW106100900A
Authority: TW
Inventors: 褚俊傑; 邱建民; 王舒眉; 蔡朋呈; 王偉丞
Original assignee: 台建生技股份有限公司
Priority date: 2017-01-11
Filing date: 2017-01-11
Publication date: 2018-07-16
Also published as: TWI639579B

Abstract

The present invention provides a use of the algae broken-wall fermentate as a foliar fertilizer, wherein the cellulolytic enzyme secreted by bacteria is utilized to hydrolyze the cell wall of the microalgae, so as to gently release the nutrients and elements of the microalgae, and collect these ingredients as a stock solution of algae broken wall fermentate. The algae broken-wall fermentate can be applied to crops or plants to promote the growth of stems, leaves and/or fruits thereof.

Description

藻類破壁發酵物作為葉面肥之用途Use of algae-broken fermented material as foliar fertilizer

本發明係關於一種藻類其細胞壁被分解後之發酵物，尤其是關於一種使用菌體將微藻類細胞壁破壁後所收集之發酵物作為葉面肥之用途。The invention relates to a fermented product of algae whose cell wall is decomposed, and in particular to a use of fermented material collected after breaking a microalgae cell wall using bacterial cells as foliar fertilizer.

一般情況下，無論是經濟作物例如禾穀類、瓜果類、蔬菜類或是觀賞植物盆栽，為使其開花數多、結果數多而品質好，肥料的使用是最常見且方便的方法。傳統上最常使用的肥料性質上屬於化學肥料，但化學肥料肥效單一、容易造成環境污染，長期使用更會導致土壤被破壞，使土質不再適合作物或植株生長。因此，為使農業能夠永續經營，不依賴大量化學肥料及化學農藥的農業生產體系是必要的，如此才能減少對環境生態的衝擊，此外，生產的能源及成本可以降低，但仍可維持穩定產量，甚至提高收益更是永續農業所追求的目標。In general, whether it is a commercial crop such as cereals, fruits, vegetables, or ornamental plants, the use of fertilizers is the most common and convenient method to increase the number of flowers, the number of results, and the quality. Traditionally, the most commonly used fertilizers are chemical fertilizers in nature, but chemical fertilizers have a single fertilizer effect and are easy to cause environmental pollution. Long-term use will cause soil damage and make soil quality no longer suitable for crops or plants. Therefore, in order to enable agriculture to operate sustainably, an agricultural production system that does not rely on a large amount of chemical fertilizers and chemical pesticides is necessary, so as to reduce the impact on the environment and ecology. In addition, the energy and cost of production can be reduced, but still stable Yield and even increase of income are the goals pursued by sustainable agriculture.

農業生產若不依賴化學肥料，就需替代以生物性肥料、有機質肥料及礦物質肥料，但以生物性肥料促進化學、有機質肥料利用效率之技術則是目前最受重視的土壤生物技術。最早的生物性肥料，是農民將蔬果採收完畢後，再焚地耕種，近年來則是利用豆科植物與穀類作物進行輪作。生物性肥料係指含具有活性微生物體(包含其休眠孢子)，如細菌、真菌、藻類等微生物之微生物體及其代謝產物的特定製劑，其具有供應植物養分的效果，例如增進植物養分和元素之供應量與總量、或刺激植物生長、或促進植物對營養和元素的吸收。因此，發現並利用一含有豐富前述有效成分且易取得或保存之生物性天然有機肥料，將是土壤生物技術發展的一重要課題。If agricultural production does not rely on chemical fertilizers, biological fertilizers, organic fertilizers and mineral fertilizers need to be replaced, but the technology of promoting the utilization efficiency of chemical and organic fertilizers with biological fertilizers is currently the most valued soil biotechnology. The earliest biological fertilizers were farmers who harvested vegetables and fruits and then burned them for cultivation. In recent years, they have used leguminous plants and gluten crops to grow crops. Biological fertilizer refers to a specific preparation containing microorganisms with active microorganisms (including their dormant spores), such as bacteria, fungi, algae, and their metabolites, which have the effect of supplying plant nutrients, such as enhancing plant nutrients and elements The amount and total supply, or stimulate plant growth, or promote plant absorption of nutrients and elements. Therefore, the discovery and utilization of a biological natural organic fertilizer that is rich in the aforementioned effective ingredients and is easily obtained or stored will be an important subject for the development of soil biotechnology.

所謂葉面肥，係指施用於植物葉面的肥料。相較於一般土壤施肥，葉面施肥較常被利用於例如作物出現養分缺乏的症狀、因土壤太酸、太鹼、太密實所造某些元素欠缺、大雨積水或乾旱所產生養分輸送不良而導致養分失衡，或目的性的促進作物的生產。於現今講求精準農業、高品質農產品、保護環境、降低用藥與節約能源的趨勢下，葉面施肥某種程度上是一相當有效之手段。基本上，調整作物生長可利用肥料成分之改變以刺激作物自身分泌生長素/荷爾蒙，而改變其原本生理作用而達到促進生長之目的，雖然使用葉面肥做為調整作物生理改變之效率遠不及直接採用植物荷爾蒙，然而其對人的安全性高，對作物生長控制之風險亦比較小。此外，使用植物荷爾蒙特別需要注意其有效濃度的範圍，而此範圍又常受植物本身營養及生理狀況、氣候狀況以及土壤中肥料狀況所影響，若濃度不適當對作物的傷害程度將相當嚴重。因此，找尋一種安全、有效，且不容易對植物產生負面影響之葉面肥亦是一必須解決的問題。此外，若能找尋到一種可同時用作葉面肥與一般根肥的肥料組成分，對於作物或植栽的培養將更能依據該植物特性與生長環境等因子來調整施用方式，而更可促進其最佳的生長。The so-called foliar fertilizer refers to the fertilizer applied to the foliage of plants. Compared with normal soil fertilization, foliar fertilization is more commonly used for symptoms such as crops showing nutrient deficiencies, lack of certain elements created by soils that are too acidic, too alkaline, and too dense, and poor nutrient transport due to heavy rain or water Causes nutrient imbalance, or promotes crop production purposefully. With today's trend of precision agriculture, high-quality agricultural products, protecting the environment, reducing drug use and saving energy, foliar fertilization is a rather effective method to a certain extent. Basically, adjustment of crop growth can utilize changes in fertilizer composition to stimulate the crop to secrete auxin / hormones, and change its original physiological role to promote growth, although the use of foliar fertilizers to adjust the physiological changes of crops is far less efficient than direct Plant hormones are used, but they are highly safe for humans and the risk of crop growth control is relatively small. In addition, the use of plant hormones requires special attention to its effective concentration range, and this range is often affected by the plant's nutritional and physiological conditions, climatic conditions and the status of fertilizers in the soil. If the concentration is not appropriate, the degree of damage to the crop will be very serious. Therefore, finding a foliar fertilizer that is safe, effective and does not easily affect the plants is also a problem that must be solved. In addition, if a fertilizer composition that can be used as both foliar fertilizer and general root fertilizer can be found, the cultivation of crops or plants will be able to adjust the application method based on factors such as the characteristics of the plant and the growth environment, and it will also promote Its the best growth.

為了解決前述的課題，找尋並利用一具較佳效果且安全之生物性肥料，幫助農民或植栽者，能夠在相當或更少的成本下，讓作物或植物的生長需求獲得滿足或提升，同時能夠在環境保護的前提下，達成永續農業之目標，實現經濟獲益與環境維護雙贏的局面，本發明提供一種藻類破壁發酵物，並將其施用於植物而作為肥料的用途。該藻類破壁發酵物係由以下方法製得：將一預定數量之微藻加入一預先培養且與該微藻具一預定比例之菌體溶液中進行發酵，其中，該菌體溶液中之菌體可分泌纖維素水解酵素；該微藻加入後使該菌體分泌該纖維素水解酵素以水解該微藻之細胞壁；以及收集該微藻於細胞壁水解後所形成之一藻類破壁發酵物原液。In order to solve the aforementioned problems, find and use a better effect and safe biological fertilizer to help farmers or growers to meet or increase the growth needs of crops or plants at a considerable or less cost. At the same time, under the premise of environmental protection, the goal of sustainable agriculture can be achieved, and a win-win situation of economic benefits and environmental maintenance can be achieved. The present invention provides an algae-broken wall ferment and applies it to plants as a fertilizer. The algae-broken fermentation product is prepared by adding a predetermined amount of microalgae to a bacterial cell solution that is cultured in advance and has a predetermined ratio with the microalgae, and wherein the bacteria in the bacterial cell solution are fermented. The cell can secrete cellulose hydrolyzing enzyme; after the microalgae is added, the cell body secretes the cellulose hydrolyzing enzyme to hydrolyze the cell wall of the microalgae; and the algae wall-broken fermented stock solution is formed after the microalgae is hydrolyzed on the cell wall .

在本發明的一實施例中，所述之藻類破壁發酵物作為葉面肥之用途，其中該菌體可為真菌。所述真菌可為杏鮑菇菌(Pleurotus eryngii )或秀珍菇菌(Pleurotus ostreatus )，但並不以此為限。In an embodiment of the present invention, the algae-broken wall fermented matter is used as a foliar fertilizer, and the fungus body can be a fungus. The fungus may be Pleurotus eryngii or Pleurotus ostreatus , but is not limited thereto.

在本發明的一實施例中，所述之藻類破壁發酵物作為葉面肥之用途，其中該微藻可為普通小球藻(Chlorella vulgaris )、原始(核)小球藻(Chlorella protothecoides )、蛋白核小球藻(Chlorella pyrenoidosa )、橢圓核小球藻(Chlorella ellipsodiea )、微小小球藻(Chlorella minutissima )或酪蠅小球藻(Chlorella protophila ) ，但並不僅限於此。In one embodiment of the present invention, the algae-broken fermented matter is used as a foliar fertilizer, wherein the microalgae may be Chlorella vulgaris , Chlorella protothecoides , Chlorella pyrenoidosa , Chlorella ellipsodiea , Chlorella minutissima , or Chlorella protophila , but it is not limited to this.

在本發明的一實施例中，所述之藻類破壁發酵物作為葉面肥之用途，其中該藻類破壁發酵物可施用於果實類作物，例如：木瓜、番茄、葡萄或香蕉植株，但並不僅限於此。In an embodiment of the present invention, the algae-broken fermented material is used as a foliar fertilizer, wherein the algae-broken fermented material can be applied to fruit crops, such as: papaya, tomato, grape or banana plants, but Not only that.

在本發明的另一態樣中，所述之藻類破壁發酵物作為葉面肥之用途，其中該藻類破壁發酵物可施用於禾穀、根莖類作物，例如：高粱、一條根、玉米、小麥，或園藝植株，例如茶花，但並不僅限於此。In another aspect of the present invention, the algae-broken fermented matter is used as a foliar fertilizer, wherein the algae-broken fermented matter can be applied to cereals and rhizome crops, such as sorghum, a root, corn, Wheat, or horticultural plants, such as camellia, are not limited to this.

在本發明的另一態樣中，所述之藻類破壁發酵物作為葉面肥之用途，其中該藻類破壁發酵物可施用於葉菜類作物，例如：萵苣、黑尼龍菜、小白菜、芥藍菜或白芥藍菜植株，但並不僅限於此。In another aspect of the present invention, the algae-broken fermented matter is used as a foliar fertilizer, wherein the algae-broken fermented matter can be applied to leafy crops, such as: lettuce, black nylon, pakchoi, kale Vegetables or white kale plants, but not limited to them.

在本發明的另一實施例中，所述之藻類破壁發酵物作為葉面肥之用途，其中該藻類破壁發酵物係施用於植株之葉面表面、背面或二者。In another embodiment of the present invention, the algae-broken fermented material is used as foliar fertilizer, wherein the algae-broken fermented material is applied to the leaf surface, the back surface, or both of the plant.

在本發明的另一實施例中，所述之藻類破壁發酵物作為葉面肥之用途，其中該藻類破壁發酵物施用之濃度係以該藻類破壁發酵物原液稀釋500倍至3000倍，較佳為稀釋500倍至2000倍，最佳為500倍至1000倍。In another embodiment of the present invention, the algae wall-broken fermented material is used as a foliar fertilizer, wherein the application concentration of the algae wall-broken fermented material is diluted 500 times to 3000 times with the algae wall-broken fermented stock solution, It is preferably diluted 500 times to 2000 times, and most preferably 500 times to 1000 times.

藉由本發明將藻類破壁發酵物作為葉面肥之用途，一方面可藉由該藻類所具有豐富之有機化合物，特別是胺基酸，或是萃取物中其他的有效成分，提供作物或植栽最適當的養分，並由葉面達到最好的吸收方式與利用，達到克服養分缺乏、因應生長環境變異或調整生長需求的目的，一方面也因為該藻類破壁發酵物對人畜無毒無害、對環境無汙染，也實現了永續農業的目標。By using the algae-broken fermented material as foliar fertilizer according to the present invention, on the one hand, the algae can provide crops or plants with abundant organic compounds, especially amino acids, or other active ingredients in the extract. The most appropriate nutrient, and the best absorption and utilization from the leaf surface, to overcome the lack of nutrients, respond to the growth environment changes or adjust the growth needs, on the one hand, because the algae-broken ferment is non-toxic and harmless to humans and animals, and The environment is pollution-free, and the goal of sustainable agriculture is also achieved.

以下將進一步說明本發明的實施方式，下述所列舉的實施例係用以闡明本發明，並非用以限定本發明之範圍，任何熟習此技藝者，在不脫離本發明之精神和範圍內，當可做些許更動與潤飾，因此本發明之保護範圍當視後附之申請專利範圍所界定者為準。The embodiments of the present invention will be further described below. The examples listed below are intended to clarify the present invention and are not intended to limit the scope of the present invention. Anyone skilled in the art will not depart from the spirit and scope of the present invention. As some changes and retouching can be done, the scope of protection of the present invention shall be determined by the scope of the attached patent application.

本發明所提供之一種藻類破壁發酵物作為葉面肥之用途，首先進行藻類破壁發酵物的製備。實施例1 藻類破壁發酵物的製備The application of the algae wall-broken fermented material as foliar fertilizer provided by the present invention firstly prepares the algae wall-broken fermented material. Example 1 Preparation of algae-broken wall fermentation

藻類破壁發酵物的製備，首先分別準備菌類溶液與藻類溶液。本實施例所利用之菌類屬真菌類的杏鮑菇菌 (Pleurotus eryngii )。將市售的杏鮑菇菌以刀子切開約5 mm正方形塊狀接種於固態培養基上 (15 g/L麥芽精粉、20 g/L葡萄糖、6 g/L酵母萃取物與15 g/L 瓊脂)，培養約7天。之後將前述瓊脂培養基挖出，加入250 ml無菌水進行均質化後備用；關於藻類部分，本實施例則係取0.5 g的綠藻粉 (Chlorella vulgaris )加入10 ml (重量比約1:20)的無菌水中，攪拌均勻後備用。取前述10 ml均質化後的杏鮑菇菌液，加入80 ml的液態培養液中 (15 g/L麥芽精粉、20 g/L葡萄糖與6 g/L酵母萃取物)，再加入10 ml綠藻溶液，之後於50 L發酵桶中，於室溫下 (25~35°C)，進行厭氧發酵約3天，即可獲得藻類破壁發酵物原液。若須以乾燥狀態取用，則可將此原液以70°C進行烘乾。For the preparation of algae-broken fermentation, firstly prepare a fungal solution and an algae solution, respectively. The fungi used in this embodiment are Pleurotus eryngii . A commercially available Pleurotus eryngii was cut with a knife to cut about 5 mm square pieces and inoculated on a solid medium (15 g / L malt powder, 20 g / L glucose, 6 g / L yeast extract and 15 g / L Agar) and cultured for about 7 days. After that, the aforementioned agar medium was dug out, and 250 ml of sterile water was added for homogenization and then used; for the algae part, in this embodiment, 0.5 g of Chlorella vulgaris was added to 10 ml (weight ratio about 1:20) Sterile water, stir well and set aside. Take the aforementioned 10 ml of homogenized Pleurotus eryngii mushroom liquid and add it to 80 ml of liquid culture medium (15 g / L malt powder, 20 g / L glucose and 6 g / L yeast extract), and then add 10 ml of green algae solution, followed by anaerobic fermentation in a 50 L fermentation tank at room temperature (25 ~ 35 ° C) for about 3 days, to obtain the algae wall-broken fermented stock solution. If it needs to be taken in a dry state, this stock solution can be dried at 70 ° C.

本發明關於藻類破壁發酵物的製備，係利用菌體所分泌的纖維素水解酵素對藻類細胞壁進行水解，相較於研磨或切割的機械方式，或是利用反覆冷凍、超音波震盪的物理方法、還是利用特殊酵素分解、有機溶劑滲透的化學方法，利用本發明生物自然分泌的纖維素水解酵素，更能夠在高效率分解細胞壁的同時，較為完整並保留該藻類內含的所有成分，而可充分利用其內容物所帶來的功效。The invention relates to the preparation of algae-broken fermented material, which uses cellulolytic enzymes secreted by the bacteria to hydrolyze the algae cell wall, compared with the mechanical method of grinding or cutting, or the physical method of repeated freezing and ultrasonic vibration Or it is a chemical method that uses special enzyme decomposition and organic solvent penetration, and uses the cellulose hydrolytic enzyme naturally secreted by the organism of the present invention, which can more effectively decompose the cell wall, and at the same time, it is more complete and retains all the ingredients contained in the algae. Make the most of its contents.

前述所利用的綠藻粉中之綠藻又稱小球藻、綠球藻，係一種微藻，生長在新鮮、無污染的水域，屬單細胞植物，細胞大小約只有2～8 µm。綠藻具有植物的兩大特徵：具有葉綠體及細胞壁，其葉綠體中含有葉綠素a及b、葉紅素、葉黃素等，而其光合作用的方式和高等植物相同。綠藻在分類學上屬植物界(Kingdom Plantae)、綠藻門(Division Chlorophyta)、綠球藻綱(Class Chlorophyceae)、綠球藻目(OrderChlorocaccales)、綠球藻科(Family Chlorellaceae)的綠球藻屬(GeneraChlorella)。可利用的綠球藻除前述普通小球藻(Chlorella vulgaris )外，尚包括：原始(核)小球藻(Chlorella protothecoides )、蛋白核小球藻(Chlorella pyrenoidosa )、橢圓核小球藻(Chlorella ellipsodiea )、微小小球藻(Chlorella minutissima )或酪蠅小球藻(Chlorella protophila )等，但並不僅限於此。The algae in the green algae powder used above are also called chlorella and chlorella, which is a kind of microalgae. It grows in fresh, unpolluted waters and belongs to single-cell plants. The cell size is only about 2-8 μm. Diarrhea has the main characteristics of plants: it has carcasses and cell walls, and its carcass contains carnosine a and b, crocetin, lutein, etc., and its photosynthesis method is the same as that of higher plants. On the basis of taxonomy, diatomaceae belongs to the Kingdom Plantae, Division Chlorophyta, Class Chlorophyceae, OrderChlorocaccales, and Family Chlorellaceae. Algae (GeneraChlorella). In addition to the aforementioned common chlorella ( Chlorella vulgaris ), available chlorella also includes: Chlorella protothecoides , Chlorella pyrenoidosa , Chlorella ellipsodiea ), Chlorella minutissima , Chlorella protophila , etc., but it is not limited to this.

前述纖維素水解酵素，可將不具溶解性的纖維素分解成單糖。該些酵素可包括但不限於：內切型纖維素纖維分解酵素(endo-β-1,4-glucanase) E.C.3.2.1.4 (又稱β-1,4-D-glucanhydrolase或carboxymethylcellulase (CMCase))、外切型纖維素纖維分解酵素(exo-β-1,4-glucanase) E.C.3.2.1.91 (又稱β-1,4-glucancellobiohydrolase或cellulose-β-1,4- glucancellobiosidase)、β-葡萄糖甘酵素(β-1,4-glucosidase) E.C.3.2.1.21(又稱β-1,4-glucohydrolase或cellobiase)。The cellulose-hydrolyzing enzyme can decompose cellulose without solubility into monosaccharides. These enzymes may include, but are not limited to, endo-β-1,4-glucanase EC3.2.1.4 (also known as β-1,4-D-glucanhydrolase or carboxymethylcellulase (CMCase)) Exo-β-1,4-glucanase EC3.2.1.91 (also known as β-1,4-glucancellobiohydrolase or cellulose-β-1,4-glucancellobiosidase), β-glucose Enzyme (β-1,4-glucosidase) EC3.2.1.21 (also known as β-1,4-glucohydrolase or cellobiase).

前述纖維素水解酵素可由不同的菌體所分泌，例如秀珍菇菌(Pleurotus ostreatus) 可分泌水解羧甲基纖維素(Carboxymethylcellulose, CMC)。The aforementioned cellulose-hydrolyzing enzyme can be secreted by different bacteria, for example, Pleurotus ostreatus can secrete hydrolyzed carboxymethyl cellulose (Carboxymethylcellulose, CMC).

前述杏鮑菇菌僅為例示，其亦可為真菌類的秀珍菇菌(Pleurotus ostreatus )，或其他可以分泌前述將藻類細胞壁加以水解的酵素的菌株。The aforementioned Pleurotus eryngii is merely an example, and it may also be a fungus Pleurotus ostreatus , or another strain that can secrete the aforementioned enzyme that hydrolyzes the cell wall of algae.

前述微藻溶液與菌體溶液的比例並未設有特別的限制，可在0.5：1~1：5的範圍內，較佳為1：1~1：3之間，更佳為1：1。實施例2 葉面肥之施用測試-木瓜The ratio of the aforementioned microalgae solution to the bacterial cell solution is not particularly limited, and may be in the range of 0.5: 1 to 1: 5, preferably between 1: 1 to 1: 3, and more preferably 1: 1. . Example 2 Application Test of Foliar Fertilizer-Papaya

選取40株台農二號木瓜株分為對照組與實驗組於網室中進行試驗。對照組使用一般化學肥料(市售即溶複合肥料10-20-20)。取10公斤該肥料以10噸水稀釋，即稀釋1000倍，以根部灌注方式進行施肥。施用週期為每5~10天灌注1次，(共進行2個月)，共施灑6次。實驗組則取200ml如實施例1所製備的藻類破壁發酵物原液，以200公升的水稀釋，及稀釋1000倍，又另取200ml如實施例1所製備的藻類破壁發酵物原液，以400公升的水稀釋，即稀釋2000倍，分別以葉面噴灑方式進行施肥。施用週期為每5~10天1次(共進行2個月)，共施灑6次。自第1次施肥後第2、4週進行紀錄，觀察其生長點(芽點)間距、開花與結果狀況，其結果如表1與圖1所示。表1 Forty papaya strains of Tainong No. 2 were selected and divided into a control group and an experimental group for testing in a net room. The control group used general chemical fertilizers (commercially available instant compound fertilizers 10-20-20). Take 10 kg of this fertilizer and dilute it with 10 tons of water, that is, dilute it 1000 times, and fertilize by root perfusion. The application cycle was perfusion every 5-10 days (for a total of 2 months), and a total of 6 sprays were applied. The experimental group took 200 ml of the algae wall-broken fermented stock solution prepared in Example 1, diluted with 200 liters of water, and diluted 1000 times. 400 liters of water was diluted, that is, 2000 times diluted, and fertilized by foliar spraying. The application cycle was once every 5 to 10 days (for a total of 2 months), and a total of 6 times were applied. Records were made at the 2nd and 4th weeks after the first fertilization, and the growth point (bud point) spacing, flowering, and fruiting conditions were observed. The results are shown in Table 1 and FIG. 1. Table 1

由表1與圖1之結果可知，雖然實驗組芽點增加的比例不如對照組，但是就結果數目而言，其結果數的比例顯著高於對照組。顯見利用本發明之藻類破壁發酵原液做為葉面肥以促進木瓜的結果，比使用一般化學肥料之效果更為顯著。實施例3 葉面肥之施用測試-茶花From the results in Table 1 and Figure 1, it can be seen that although the percentage of sprouting points in the experimental group is not as good as that in the control group, the proportion of the number of results is significantly higher than that in the control group. It is obvious that the use of the algae-broken fermentation fermentation liquid of the present invention as a foliar fertilizer to promote the results of papaya is more significant than the use of general chemical fertilizers. Example 3 Application Test of Foliar Fertilizer-Camellia

取700顆7吋茶花苗分為對照組與實驗組進行試驗。對照組使用一般化學肥料(市售即溶複合肥料10-20-20)。取10公斤該肥料以10噸水稀釋，即稀釋1000倍，以土壤灌注方式進行施肥。施用週期為每月灌注1次(6月~11月間)，共6次。實驗組則取200 ml如實施例1所製備的藻類破壁發酵物原液，以200公升的水稀釋，即稀釋1000倍，以葉面噴灑方式進行施肥。施用週期為每15天施灑1次(共進行2個月)，共施灑4次。自第1次施肥後第60天進行紀錄，觀察葉、莖等生長狀況，其結果如表2與圖2所示。表2 700 7-inch camellia seedlings were divided into a control group and an experimental group for testing. The control group used general chemical fertilizers (commercially available instant compound fertilizers 10-20-20). Take 10 kg of this fertilizer and dilute it with 10 tons of water, that is, diluted 1000 times, and fertilize by soil infusion. The application cycle is 6 times per month (from June to November). In the experimental group, 200 ml of the algae-broken fermented stock solution prepared in Example 1 was diluted with 200 liters of water, that is, diluted 1,000 times, and fertilized by foliar spraying. The application cycle was once every 15 days (for a total of 2 months) and 4 times in total. Records were made on the 60th day after the first fertilization, and the growth conditions of leaves and stems were observed. The results are shown in Table 2 and Figure 2. Table 2

由表2與圖2之結果可知，在葉的形態部分，對照組與實驗組的葉色都可呈現深綠色，但實驗組的葉面面積較大，而且嫩芽相較於只有少數幾顆有的對照組，實驗組每顆幾乎都有嫩芽，整體生長情況佳。在莖生長方面，對照組一開始並未有明顯差異，一直到第45天才有明顯成長，枝長平均為75公分，莖直徑平均為1.1公分；至於對照組，枝幹成長速度並未有停滯現象，從施灑後就有逐漸成長，枝長平均為95公分，莖直徑平均為1.4公分。因此，最後整株茶花重量，實驗組平均為2.3公斤，相較之下，對照組只有1.8公斤，足足少了約27%的重量。從以上結果可知，藉由本發明之施用方法，特別是利用藻類破壁發酵物作為葉面肥之利用方式，的確能夠有效產生生長促進之效果。相較於過去習知藻類僅作為營養補充品、促進免疫活性等保健食品 (請參江善宗等著，纖維素水解酵素於綠藻工業之應用研究，2006，農業生技產業季刊第七期)而言，本發明提供一完全異於習知的效用。實施例4 葉面肥之施用測試-聖女番茄From the results in Table 2 and Figure 2, it can be seen that in the morphological part of the leaves, the leaf color of the control group and the experimental group can be dark green, but the leaf area of the experimental group is larger, and the buds are smaller than those of only a few. In the control group and the experimental group, almost all of them had buds, and the overall growth was good. In terms of stem growth, there was no significant difference in the control group at the beginning, and there was no significant growth until the 45th day. The average branch length was 75 cm and the average stem diameter was 1.1 cm. As for the control group, the growth rate of the stem did not stagnate. It has gradually grown since application, with an average branch length of 95 cm and an average stem diameter of 1.4 cm. Therefore, the weight of the last camellia plant averaged 2.3 kg in the experimental group, compared with 1.8 kg in the control group, which was about 27% less in weight. From the above results, it can be known that, with the application method of the present invention, in particular, the use of the algae-broken fermented material as a utilization method of foliar fertilizer can indeed effectively produce the effect of promoting growth. Compared to the past, algae has only been used as nutritional supplements, and to promote immune activity and other health foods (see Jiang Zongzong et al., Application research of cellulose hydrolase in green algae industry, 2006, Agricultural Biotechnology Industry Quarterly Issue 7) In other words, the present invention provides a completely different utility. Example 4 Application Test of Foliar Fertilizer

以300坪園區分對照組與實驗組二個區域進行試驗。對照組使用一般化學肥料(市售即溶複合肥料10-20-20)。取1公斤該肥料以1噸水稀釋，即稀釋1000倍以土壤灌注方式進行施肥。施用週期為每月灌注1次，共6次。實驗組則取200 ml如實施例1所製備的藻類破壁發酵物原液，以200公升的水稀釋，即稀釋1000倍，於種植芽苗三週後著果期時，以葉面噴灑方式進行施肥。施用週期為每15天施灑1次(共進行2個月)，共施灑4次。自第1次施肥後第60天進行紀錄，觀察葉、莖等生長狀況，其結果如表3與圖3所示。表3 The 300-pingping area was divided into two groups: the control group and the experimental group. The control group used general chemical fertilizers (commercially available instant compound fertilizers 10-20-20). Take 1 kg of this fertilizer and dilute it with 1 ton of water, that is, dilute it 1000 times and fertilize by soil infusion. The application cycle is 6 times per month. In the experimental group, 200 ml of the algae-broken fermented stock solution prepared in Example 1 was diluted with 200 liters of water, that is, diluted 1,000 times. When the seedlings were planted three weeks after the fruiting period, the leaves were sprayed. Fertilize. The application cycle was once every 15 days (for a total of 2 months) and 4 times in total. Records were made on the 60th day after the first fertilization, and the growth conditions of leaves and stems were observed. The results are shown in Table 3 and Figure 3. table 3

由表3與圖3之結果可知，在果實形態部分，對照組的果實較為瘦扁、大小不一、且光澤黯淡偏黃，但實驗組相較之下，果實相當飽滿、大小較均勻一致、且顏色紅潤。此外，就果實口感測試，對照組較軟嫩、水分較少，但實驗組之果實因飽滿而較Q脆，而且水分多，整體果實品質優良。另一方面，就產量而言對照組結果數多，總收成僅為280公斤，而實驗組為350公斤，提高產量約25%。此外，實驗組之甜度亦大幅提高4度。從以上結果可知，藉由本發明之施用方法，特別是利用藻類破壁發酵物作為葉面肥之利用方式，的確能夠有效促進果實的生長，並使其具有好的品質、甜度，同樣是完全異於習知的新效用。實施例5 葉面肥之施用測試-玉米From the results in Table 3 and Figure 3, in the fruit morphology part, the fruits of the control group are thinner, flat, different in size, and dim and yellowish, but compared with the experimental group, the fruits are quite full, the size is uniform, And the color is ruddy. In addition, as for the fruit taste test, the control group was softer and tenderer and had less water, but the fruit in the experimental group was fuller and more brittle than Q, and had more water, and the overall fruit quality was excellent. On the other hand, in terms of output, the control group had more results, with a total yield of only 280 kg, while the experimental group was 350 kg, which increased output by about 25%. In addition, the sweetness of the experimental group also increased significantly by 4 degrees. From the above results, it can be seen that, with the application method of the present invention, in particular, the use of algae-broken fermented material as a method of foliar fertilizer can indeed effectively promote the growth of fruits and make them have good quality and sweetness, which is also completely different. New utility in learning. Example 5 Application test of foliar fertilizer-corn

以2公頃田地分對照組與實驗組二個區域進行試驗。對照組使用一般化學肥料(市售即溶複合肥料10-20-20)，取10公斤該肥料以10噸水稀釋，即稀釋1000倍，以葉面噴灑方式進行施肥。施用週期為每10天施灑1次(共進行6個月)，共施灑18次。實驗組使用10 L如實施例1所製備的藻類破壁發酵原液，以10公噸的水稀釋，即稀釋1000倍，以葉面噴灑方式進行施肥。施用週期為每10天施灑1次(共進行6個月)，共施灑18次。其生長情形如表4所示。表4 A two-hectare field was divided into two areas, the control group and the experimental group. The control group used general chemical fertilizers (commercially-available instant compound fertilizers 10-20-20), took 10 kg of this fertilizer and diluted it with 10 tons of water, that is, diluted 1,000 times, and applied the fertilizer by foliar spraying. The application cycle was once every 10 days (for a total of 6 months) and 18 times in total. The experimental group used 10 L of the algae-broken wall fermentation fermentation solution prepared in Example 1, diluted with 10 metric tons of water, that is, diluted 1000 times, and fertilized by foliar spraying. The application cycle was once every 10 days (for a total of 6 months) and 18 times in total. The growth situation is shown in Table 4. Table 4

由表4可知，實驗組玉米植株的莖生長得較為粗壯，每莖增重高達77%，而每莖玉米粒數也有高達69%的增長。因此，實驗組的玉米每公頃產量大幅提高了45%。顯見利用本發明之藻類破壁發酵原液做為肥料用途，將比一般化學肥料之效果更為顯著。實施例6 葉面肥之施用測試-小麥It can be known from Table 4 that the stems of the corn plants in the experimental group grew thicker, with each stem gaining as much as 77%, and the number of kernels per stem also increasing as much as 69%. As a result, corn yield per hectare in the experimental group increased significantly by 45%. It is obvious that the use of the algae-broken fermentation broth of the present invention as a fertilizer will have a more significant effect than that of general chemical fertilizers. Example 6 Application test of foliar fertilizer-wheat

以2公頃田地分對照組與實驗組二個區域進行試驗。對照組使用一般化學肥料(市售即溶複合肥料10-20-20)，取10公斤該肥料以10噸水稀釋，即稀釋1000倍，以葉面噴灑方式進行施肥。施用週期為每10天施灑1次(共進行4個月)，共施灑12次。實驗組使用10 L如實施例1所製備的藻類破壁發酵原液，以10公噸的水稀釋，即稀釋1000倍，以葉面噴灑方式進行施肥。施用週期為每10天施灑1次(共進行4個月)，共施灑12次。其生長情形如表5所示。表5 A two-hectare field was divided into two areas, the control group and the experimental group. The control group used general chemical fertilizers (commercially-available instant compound fertilizers 10-20-20), took 10 kg of this fertilizer and diluted it with 10 tons of water, that is, diluted 1,000 times, and applied the fertilizer by foliar spraying. The application cycle was once every 10 days (for a total of 4 months) and a total of 12 times. The experimental group used 10 L of the algae-broken wall fermentation fermentation solution prepared in Example 1, diluted with 10 metric tons of water, that is, diluted 1000 times, and fertilized by foliar spraying. The application cycle was once every 10 days (for a total of 4 months) and a total of 12 times. The growth situation is shown in Table 5. table 5

由表5可知，實驗組小麥植株所結的穀穗較為飽滿，穀重平均增加了40%，而整體產量大幅提高到140%，幾乎為原本的2.5倍。顯見利用本發明之藻類破壁發酵原液做為肥料用途，將比一般化學肥料之效果更為顯著。實施例7 葉面肥之施用測試-葡萄It can be known from Table 5 that the ears of wheat produced by the wheat plants in the experimental group are relatively full, the average grain weight increased by 40%, and the overall yield increased significantly to 140%, which is almost 2.5 times the original. It is obvious that the use of the algae-broken fermentation broth of the present invention as a fertilizer will have a more significant effect than that of general chemical fertilizers. Example 7 Application Test of Foliar Fertilizer-Grape

以2公頃田地分對照組與實驗組二個區域進行試驗。對照組使用一般化學肥料(市售即溶複合肥料10-20-20)，取10公斤該肥料以10噸水稀釋，即稀釋1000倍，以葉面噴灑方式進行施肥。施用週期為每10天施灑1次(共進行7個月)，共施灑21次。實驗組使用10 L如實施例1所製備的藻類破壁發酵原液，以10公噸的水稀釋，即稀釋1000倍，以葉面噴灑方式進行施肥。施用週期為每10天施灑1次(共進行7個月)，共施灑21次。其生長情形如表6所示。表6 A two-hectare field was divided into two areas, the control group and the experimental group. The control group used general chemical fertilizers (commercially-available instant compound fertilizers 10-20-20), took 10 kg of this fertilizer and diluted it with 10 tons of water, that is, diluted 1,000 times, and applied the fertilizer by foliar spraying. The application cycle was once every 10 days (for a total of 7 months) and 21 times in total. The experimental group used 10 L of the algae-broken fermentation broth prepared in Example 1, diluted with 10 metric tons of water, that is, diluted 1000 times, and applied fertilizer by foliar spraying. The application cycle was once every 10 days (for a total of 7 months) and 21 times in total. The growth situation is shown in Table 6. Table 6

由表6可知，實驗組葡萄植株每一藤蔓同樣生長得較為粗壯且長，每藤蔓增重30%，而每公頃的產量似乎與藤蔓的生長相關，也增加了約25%。重要的是，葡萄的甜度由原本的14度大幅提高至19度。因此，利用本發明之藻類破壁發酵原液做為肥料用途，將比一般化學肥料之效果更為顯著，對於果實的甜度、品質也有明顯提升。實施例8 葉面肥之施用測試-黑尼龍菜It can be known from Table 6 that each vine of the experimental group grapes also grows stoutly and long, each vine gains 30%, and the yield per hectare seems to be related to the growth of the vine, which also increases by about 25%. Importantly, the sweetness of the grapes increased significantly from 14 degrees to 19 degrees. Therefore, the use of the algae-broken fermentation broth of the present invention as a fertilizer will have a more significant effect than general chemical fertilizers, and it will also significantly improve the sweetness and quality of the fruit. Example 8 Application Test of Foliar Fertilizer-Black Nylon

以300平方公尺田地分對照組與實驗組二個區域進行試驗。對照組使用一般化學肥料(市售即溶複合肥料10-20-20)，取50毫升該肥料以25公升水稀釋，即稀釋500倍，以葉面噴灑方式進行施肥。施用週期為每7天施灑1次(共進行1個月)，共施灑4次。試驗組使用50毫升如實施例1所製備的藻類破壁發酵原液(液態) 以25公升的水稀釋，即稀釋500倍，以葉面噴灑方式進行施肥。施用週期為每7天施灑1次(共進行1個月)，共施灑4次，其生長結果如表7與圖4所示。表7 A 300-square-meter field was divided into two areas, the control group and the experimental group. The control group used a general chemical fertilizer (commercially available instant compound fertilizer 10-20-20), and 50 ml of the fertilizer was diluted with 25 liters of water, that is, diluted 500 times, and the fertilizer was applied by foliar spraying. The application cycle was once every 7 days (for a total of 1 month) and 4 times in total. The test group used 50 ml of the algae-broken wall fermentation fermentation liquid (liquid) prepared in Example 1 to be diluted with 25 liters of water, that is, diluted 500 times, and fertilized by foliar spraying. The application cycle was once every 7 days (for a total of 1 month) and 4 times in total. The growth results are shown in Table 7 and FIG. 4. Table 7

由表7與圖4可知，黑尼龍菜實驗組中，每一植株的體型要比原本使用一般肥料壯大許多，無論葉長、葉寬、莖長、莖粗都增加了約15%~60%，且葉片數目也增加了50%，使整體的產量大幅提高。此外，觀察菜株上之蛀蟲，也發現實驗組的部分減少了7%，因此，利用本發明之藻類破壁發酵原液做為肥料用途，除了比一般化學肥料之效果更為顯著外，也提高了植株對蛀蟲的抵抗力。實施例9 葉面肥之施用測試-小白菜As can be seen from Table 7 and Figure 4, in the black nylon vegetable experimental group, the size of each plant was much larger than the original use of general fertilizers, and the leaf length, leaf width, stem length, and stem thickness increased by about 15% to 60%. , And the number of leaves also increased by 50%, so that the overall yield increased significantly. In addition, when observing tapeworms on vegetable plants, it was also found that the experimental group was reduced by 7%. Therefore, the use of the algae-broken fermentation broth of the present invention as a fertilizer is more effective than ordinary chemical fertilizers, and it also improves. The plant's resistance to roundworms. Example 9 Application Test of Foliar Fertilizer-Chinese Cabbage

以300平方公尺田地分對照組與實驗組二個區域進行試驗。對照組使用一般化學肥料(市售即溶複合肥料10-20-20)，取50毫升該肥料以25公升水稀釋，即稀釋500倍，以葉面噴灑方式進行施肥。施用週期為每7天施灑1次(共進行1個月)，共施灑4次。實驗組使用50毫升如實施例1所製備的藻類破壁發酵原液(液態) 以25公升的水稀釋，即稀釋500倍，以葉面噴灑方式進行施肥。施用週期為每7天施灑1次(共進行1個月)，共施灑4次。其生長情形如表8與圖5所示。表8 A 300-square-meter field was divided into two areas, the control group and the experimental group. The control group used a general chemical fertilizer (commercially available instant compound fertilizer 10-20-20), and 50 ml of the fertilizer was diluted with 25 liters of water, that is, diluted 500 times, and the fertilizer was applied by foliar spraying. The application cycle was once every 7 days (for a total of 1 month) and 4 times in total. The experimental group used 50 ml of the algae-broken wall fermentation fermentation solution (liquid) prepared in Example 1 to be diluted with 25 liters of water, that is, diluted 500 times, and fertilized by foliar spraying. The application cycle was once every 7 days (for a total of 1 month) and 4 times in total. The growth situation is shown in Table 8 and Figure 5. Table 8

由表8與圖5可知，小白菜實驗組中，每一植株的體型要比原本使用一般肥料壯大許多，無論葉長、葉寬、莖長、莖粗都增加了約20%~58%，且葉片數目也增加了57%，使整體的產量大幅提高。此外，觀察菜株上之蛀蟲，也發現實驗組的部分減少了5%，因此，利用本發明之藻類破壁發酵原液做為肥料用途，除了比一般化學肥料之效果更為顯著外，也提高了植株對蛀蟲的抵抗力。實施例10 葉面肥之施用測試-芥藍菜From Table 8 and Figure 5, it can be seen that in the pakchoi experimental group, the size of each plant was much larger than the original use of ordinary fertilizers. The leaf length, leaf width, stem length, and stem thickness increased by about 20% to 58%. And the number of leaves also increased by 57%, which greatly improved the overall yield. In addition, when observing tapeworms on vegetable plants, it was also found that the experimental group was reduced by 5%. Therefore, the use of the algae-broken fermentation broth of the present invention as a fertilizer is more effective than conventional chemical fertilizers, and it also improves. The plant's resistance to roundworms. Example 10 Application Test of Foliar Fertilizer-Kale

以300平方公尺田地分對照組與實驗組二個區域進行試驗。對照組使用一般化學肥料(市售即溶複合肥料10-20-20)，取50毫升該肥料以25公升水稀釋，即稀釋500倍，以葉面噴灑方式進行施肥。施用週期為每7天施灑1次(共進行1個月)，共施灑4次。試驗組使用50毫升如實施例1所製備的藻類破壁發酵原液(液態) 以25公升的水稀釋，即稀釋500倍，以葉面噴灑方式進行施肥。施用週期為每7天施灑1次(共進行1個月)，共施灑4次，其生長結果如表9與圖6所示。表9 A 300-square-meter field was divided into two areas, the control group and the experimental group. The control group used a general chemical fertilizer (commercially available instant compound fertilizer 10-20-20), and 50 ml of the fertilizer was diluted with 25 liters of water, that is, diluted 500 times, and the fertilizer was applied by foliar spraying. The application cycle was once every 7 days (for a total of 1 month) and 4 times in total. The test group used 50 ml of the algae-broken wall fermentation fermentation liquid (liquid) prepared in Example 1 to be diluted with 25 liters of water, that is, diluted 500 times, and fertilized by foliar spraying. The application cycle was once every 7 days (for a total of 1 month) and 4 times in total. The growth results are shown in Table 9 and FIG. 6. Table 9

由表9與圖6可知，芥藍菜實驗組中，每一植株的體型要比原本使用一般肥料壯大許多，無論葉長、葉寬、莖長、莖粗都增加了約28%~50%，且葉片數目也增加了33%，使整體的產量大幅提高。此外，觀察菜株上之蛀蟲，也發現實驗組的部分減少了3%，因此，利用本發明之藻類破壁發酵原液做為肥料用途，除了比一般化學肥料之效果更為顯著外，也提高了植株對蛀蟲的抵抗力。實施例11 葉面肥之施用測試-白芥藍菜From Table 9 and Figure 6, it can be seen that in the kale experiment group, the size of each plant was much larger than the original fertilizer, and the leaf length, leaf width, stem length, and stem thickness increased by about 28% to 50%. , And the number of leaves also increased by 33%, so that the overall yield increased significantly. In addition, when looking at tapeworms on vegetable plants, it was also found that the experimental group was reduced by 3%. Therefore, the use of the algae-broken wall fermentation fermentation liquid of the present invention as a fertilizer is more significant than ordinary chemical fertilizers, and it also improves. The plant's resistance to roundworms. Example 11 Application Test of Foliar Fertilizer-White Kale

以300平方公尺田地分對照組與實驗組二個區域進行試驗。對照組使用一般化學肥料，取50毫升該肥料以25公升水稀釋，即稀釋500倍，以葉面噴灑方式進行施肥。施用週期為每7天施灑1次(共進行1個月)，共施灑4次。試驗組使用50毫升如實施例1所製備的藻類破壁發酵原液(液態) 以25公升的水稀釋，即稀釋500倍，以葉面噴灑方式進行施肥。施用週期為每7天施灑1次(共進行1個月)，共施灑4次，其生長結果如表10與圖7所示。表10 A 300-square-meter field was divided into two areas, the control group and the experimental group. The control group used a general chemical fertilizer, and 50 ml of the fertilizer was diluted with 25 liters of water, that is, diluted 500 times, and the fertilizer was applied by foliar spraying. The application cycle was once every 7 days (for a total of 1 month) and 4 times in total. The test group used 50 ml of the algae-broken wall fermentation fermentation liquid (liquid) prepared in Example 1 to be diluted with 25 liters of water, that is, diluted 500 times, and fertilized by foliar spraying. The application cycle was once every 7 days (for a total of 1 month) and 4 times in total. The growth results are shown in Table 10 and FIG. 7. Table 10

由表10與圖7可知，實驗組的白芥藍菜，其葉長、葉寬、莖長、莖粗都增加了約13%~22%，雖增加幅度較小，但還是比原本使用一般肥料壯大許多。此外，葉片數目卻增加了75%，推測是葉片數之增加導致每一葉片之大小增長幅度縮小。另一方面，觀察菜株上之蛀蟲，也發現實驗組的部分同樣有減少，並減少了2%，因此，利用本發明之藻類破壁發酵原液做為肥料用途，除了比一般化學肥料之效果更佳外，也提高了植株對蛀蟲的抵抗力。As can be seen from Table 10 and Figure 7, the white kale in the experimental group increased leaf length, leaf width, stem length, and stem thickness by about 13% to 22%. Although the increase was small, it was still more general than the original use. The fertilizer grows a lot. In addition, the number of leaves increased by 75%, presumably because the increase in the number of leaves resulted in a reduction in the size of each leaf. On the other hand, when observing tapeworms on vegetable plants, it was also found that the experimental group also had a decrease of 2%, so the use of the algae-broken fermentation broth of the present invention as a fertilizer was not only more effective than ordinary chemical fertilizers In addition, it also improves the plant's resistance to roundworms.

藉由上述試驗可知，本發明以藻類破壁發酵物作為葉面肥之用途，除了在根、莖、葉與果實的生長調節上，產生促進分化與發育之效果外，亦可藉由該發酵物所含有之其他有效成分，提供植株生長所需之養分或元素，因此更能進一步促進植物各器官之發育與成長，同時由於該藻類破壁發酵物對於植株本身並無負面之生長影響，因此在濃度的控制上也較無須擔心因其不平衡所造成的植株傷害。It can be known from the above tests that the application of the algae-broken fermented material as foliar fertilizer in the present invention can not only produce the effect of promoting differentiation and development on the growth regulation of roots, stems, leaves and fruits, but also the fermented material. The other active ingredients contained provide nutrients or elements needed for plant growth, so it can further promote the development and growth of various organs of the plant.At the same time, because the algae wall-broken ferment has no negative growth impact on the plant itself, There is also less need to worry about plant damage due to its imbalance.

無no

圖1係本發明實施例施用於木瓜對其莖、葉生長影響之比較照片。圖2係本發明實施例施用於茶花對其莖、葉生長影響之比較照片。圖3係本發明實施例施用於聖女番茄對其果實生長影響之比較照片。圖4係本發明實施例施用於黑尼龍菜對其莖、葉生長影響之比較照片。圖5係本發明實施例施用於小白菜對其莖、葉生長影響之比較照片。圖6係本發明實施例施用於芥藍菜對其莖、葉生長影響之比較照片。圖7係本發明實施例施用於白芥藍菜對其莖、葉生長影響之比較照片。FIG. 1 is a comparative photograph of the effect of papaya on stem and leaf growth according to an embodiment of the present invention. FIG. 2 is a comparative photograph of the effect of camellia on the growth of its stems and leaves according to the embodiment of the present invention. FIG. 3 is a comparison photograph of the effect of applying the tomato to the fruit growth of the virgin tomato according to the embodiment of the present invention. FIG. 4 is a comparison photograph of the effect of black nylon vegetable on stem and leaf growth according to an example of the present invention. FIG. 5 is a comparison photograph of the effect of pakchoi on its stem and leaf growth according to an example of the present invention. FIG. 6 is a comparison photograph of the effect of kale on the growth of stems and leaves in the examples of the present invention. FIG. 7 is a comparison photograph of the effect of white kale on the growth of stems and leaves in the examples of the present invention.

Claims

一種藻類破壁發酵物作為葉面肥之用途，其中該藻類破壁發酵物係由以下方法製得：將一預定數量之微藻加入一預先培養且與該微藻具一預定比例之菌體溶液中進行發酵，其中，該菌體溶液中之菌體可分泌纖維素水解酵素；該微藻加入後使該菌體分泌該纖維素水解酵素以水解該微藻之細胞壁；以及收集該微藻於細胞壁水解後所形成之一藻類破壁發酵物原液。An algae-broken fermented material is used as a foliar fertilizer, wherein the algae-broken fermented material is prepared by the following method: adding a predetermined amount of microalgae to a pre-cultured bacterial cell solution having a predetermined ratio with the microalgae Fermentation, wherein the cells in the cell solution can secrete cellulose hydrolyzing enzymes; the addition of the microalgae causes the cells to secrete the cellulolytic enzymes to hydrolyze the cell wall of the microalgae; and collecting the microalgae in An algae-broken fermented stock solution formed after cell wall hydrolysis.

如申請專利範圍第1項所述之藻類破壁發酵物作為葉面肥之用途，其中該菌體係真菌。The application of the algae-broken fermented material as foliar fertilizer as described in item 1 of the patent application scope, wherein the fungus is a fungal system.

如申請專利範圍第2項所述之藻類破壁發酵物作為葉面肥之用途，其中該真菌係杏鮑菇菌(Pleurotus eryngii )或秀珍菇菌(Pleurotus ostreatus )。According to the application of the algae-broken fermented material as the foliar fertilizer as described in item 2 of the patent application scope, the fungus is Pleurotus eryngii or Pleurotus ostreatus .

如申請專利範圍第1項所述之藻類破壁發酵物作為葉面肥之用途，其中該微藻係普通小球藻(Chlorella vulgaris )、原始(核)小球藻(Chlorella protothecoides )、蛋白核小球藻(Chlorella pyrenoidosa )、橢圓核小球藻(Chlorella ellipsodiea )、微小小球藻(Chlorella minutissima )或酪蠅小球藻(Chlorella protophila )。According to the application of the algae-broken fermented material as the foliar fertilizer as described in the first patent application scope, the microalgae are Chlorella vulgaris , Chlorella protothecoides , and protein nuclei. Chlorella pyrenoidosa , Chlorella ellipsodiea , Chlorella minutissima or Chlorella protophila .

如申請專利範圍第1項所述之藻類破壁發酵物作為葉面肥之用途，其中該藻類破壁發酵物係施用於木瓜、番茄、葡萄或香蕉植株。The use of the algae-broken fermented material as the foliar fertilizer as described in item 1 of the scope of the patent application, wherein the algae-broken fermented material is applied to papaya, tomato, grape or banana plants.

如申請專利範圍第1項所述之藻類破壁發酵物作為葉面肥之用途，其中該藻類破壁發酵物係施用於高粱、一條根、玉米、茶花或小麥植株。The use of the algae-broken fermented material as the foliar fertilizer as described in item 1 of the scope of application patent, wherein the algae-broken fermented material is applied to sorghum, a root, corn, camellia or wheat plants.

如申請專利範圍第1項所述之藻類破壁發酵物作為葉面肥之用途，其中該藻類破壁發酵物係施用於萵苣、黑尼龍菜、小白菜、芥藍菜或白芥藍菜植株。The use of the algae-broken fermented material as the foliar fertilizer as described in item 1 of the scope of the patent application, wherein the algae-broken fermented material is applied to lettuce, black nylon vegetable, pakchoi, kale or white kale plant.

如申請專利範圍第1、5、6或7項所述之藻類破壁發酵物作為葉面肥之用途，其中該藻類破壁發酵物係施用於植株之葉面表面、背面或二者。For example, the use of the algae-broken fermented material as described in item 1, 5, 6, or 7 of the scope of application for the application of foliar fertilizer, wherein the algae-broken fermented material is applied to the leaf surface, back, or both of the plant.

如申請專利範圍第1項所述之藻類破壁發酵物作為葉面肥之用途，其中該藻類破壁發酵物施用之濃度係以該藻類破壁發酵物原液稀釋500倍至3000倍。According to the application of the algae wall-broken fermented material as the foliar fertilizer as described in item 1 of the scope of the application patent, the concentration of the algae wall-broken fermented material is diluted 500 times to 3000 times with the algae wall-broken fermented stock solution.