CN108834764A - A kind of preparation method of oil tea mushroom activated liquid strain - Google Patents
A kind of preparation method of oil tea mushroom activated liquid strain Download PDFInfo
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- CN108834764A CN108834764A CN201810631229.3A CN201810631229A CN108834764A CN 108834764 A CN108834764 A CN 108834764A CN 201810631229 A CN201810631229 A CN 201810631229A CN 108834764 A CN108834764 A CN 108834764A
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- oil tea
- tea mushroom
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- liquid strain
- activated liquid
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/40—Cultivation of spawn
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
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- Mycology (AREA)
- Environmental Sciences (AREA)
- Non-Alcoholic Beverages (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention discloses a kind of preparation methods of oil tea mushroom activated liquid strain, including oil tea massee fruiting bodies activation culture and fermented and cultured.The present invention, which uses, is added with ginkgo biloba p.e activation medium, energy fast activating oil tea massee fruiting bodies, and the culture for passing through fermentation broth, the ginkgo biloba p.e and conch meal of addition, and the chitin contained in conch meal can be such that oil tea mushroom strains quickly breed, the fruiting time for shortening the oil tea mushroom of inoculation, increases the yield of oil tea mushroom.
Description
Technical field
The invention belongs to edible mushroom field more particularly to a kind of preparation methods of oil tea mushroom activated liquid strain.
Background technique
Oil tea mushroom, Agroeybe cylindracea mycelia are a kind of edible and medical fungis, and cap is delicate, handle is crisp, it is pure fragrant, tasty to hide, because wild
In tea oil tree it is dried-up on also gain the name oil tea mushroom.Agroeybe cylindracea mycelia is full of nutrition, and protein content is high, containing there are many needed by human
Amino acid, and the mineral elements such as B family vitamin rich in and potassium, sodium, calcium, magnesium, iron, zinc.Agroeybe cylindracea mycelia has clear
The effect of heat, calming the liver, improving eyesight, diuresis, invigorating the spleen.Market demand increases sharply in recent years.Oil tea mushroom domestication and cultivation at
The time of function is very short, although the yield of artificial cultivation increased by a fairly big margin in recent years, since it is harvested, the period is long, and technique is opposite
It is cumbersome, it at present still in the primary pocket type cultivation stage, is influenced by season very big, is not carried out large batch of batch production and plants
Training, and strain also still traditional solid spawn, since solid spawn is mostly all in suspend mode period, the Spawn incubation time is long,
Each seed bottle can only at most be inoculated with tens cultivating bags, need to prepare a large amount of seed bottle, and cultivating bag mycelia sprouts after inoculation
Hair point is few, and speed of germinating is slow, is easy to happen miscellaneous bacteria insect pest, and cultivation cycle is long, and factory culture is difficult to realize.
It, can be to first carrying out activation and fluid enlargement culture, such as patent to oil tea mushroom strains fructification so in the prior art
CN201610472477.9 invention disclosed patent《A kind of oil tea mushroom culture medium and oil tea mushroom liquid strain system based on Juncao
Preparation Method》, use Juncao, carrot meal, yeast autolysis powder, bee milk, potassium dihydrogen phosphate, magnesium sulfate, land plaster, sophorolipid,
Calcium propionate, hydrogenated plant oil, sodium cellulose glycolate, cellulase, precipitated calcium carbonate, nano-scaled electric mountain flour, bacteria nano
The culture medium of cellulose expands culture strain, such as patent CN201510587382.7 invention disclosed patent《A kind of oil
The preparation method of tea mushroom industrialized cultivation liquid fermentation strain》, above-mentioned technology also functions to certain effect, but incubation time mistake
Long, the strain concentration cultivated is too low.
Summary of the invention
In order to solve the above technical problems, the present invention provides a kind of preparation methods of oil tea mushroom activated liquid strain, specifically
Scheme is as follows：
A kind of preparation method of oil tea mushroom activated liquid strain, includes the following steps：
1）Oil tea massee fruiting bodies activation culture, the closed culture in activation medium by oil tea massee fruiting bodies, until there is mycelia
Ball, culture environment are low pressure hyperoxia, and air pressure is 0.2-0.8 atmospheric pressure, oxygen concentration 24-30%；
2）Fermented and cultured, by step 1）Mycelium pellet be connected in the fermentation broth in fermentor and cultivate 3-5 days, and obtain
Oil tea mushroom activated liquid strain.
Further, 15-45g/L potato juice, 20-40g/L agar and 4-10g/L are contained in the activation medium
Ginkgo biloba p.e.
Further, it is mentioned in the activation medium containing 20g/L potato juice, 35g/L agar and 7g/L ginkgo leaf
Take object.
Further, the fermentation broth contains following component：10-20g/L wheat flour, 20-30g/L Hu trailing plants
Bu Fen, 35-45g/L conch meal, 0.2-5g/L ginkgo biloba p.e, 0.4-0.5g/L potassium dihydrogen phosphate, 10-20g/L wood fibre
Element, 0.3-0.4g/L magnesium sulfate, 10~20g/L sugar, 2-5g/L yeast powder.
Further, the fermentation broth contains following component：15g/L wheat flour, 27g/L carrot meal,
38g/L conch meal, 2g/L ginkgo biloba p.e, 0.45g/L potassium dihydrogen phosphate, 15g/L lignocellulosic, 0.33g/L magnesium sulfate,
15g/L sugar, 4g/L yeast powder.
Further, the ginkgo biloba p.e contains the GINKGO BILOBA EXTRACT of 20-30% and the lactones of 2-8%.
Further, the conch meal passes through 560 ~ 575 DEG C, and the internal environment calcining 1 of 0.03 ~ 0.08 atmospheric pressure ~
3h, is then polished into the sodium hydroxide solution that 10-15% is sprayed after powder, and 3 ~ 5h in 200 ~ 300 DEG C of temperature, then with phosphoric acid tune
PH value is saved to 6-6.5.
Further, the fermentation condition of the fermented and cultured is pH5.2-5.5,28-30 DEG C of temperature.
Technical effect of the invention is：It, can fast activating oil tea mushroom using ginkgo biloba p.e activation medium is added with
Fructification, and by the culture of fermentation broth, the ginkgo biloba p.e and conch meal of addition, and contain in conch meal
Some chitins can be such that oil tea mushroom strains quickly breed, and the concentration of manufactured oil tea mushroom activated liquid strain is much higher than existing skill
Art, and the fruiting time of oil tea mushroom is shortened, increase the yield of oil tea mushroom.
Specific embodiment
Be described further below technical solution of the present invention, but claimed range be not limited to it is described.
Embodiment one
The preparation method for present embodiments providing a kind of oil tea mushroom activated liquid strain, includes the following steps：
1, culture medium prepares
1）In activation medium：15g/L potato juice, 40g/L agar and 4g/L ginkgo biloba p.e, ginkgo biloba p.e contain
The GINKGO BILOBA EXTRACT of 20-30% and the lactones of 2-8%, ginkgo biloba p.e are purchased from one hundred Si Te Biotechnology Co., Ltd of Xi'an.
2）Fermentation broth：10g/L wheat flour, 30g/L carrot meal, 35g/L conch meal, 5g/L ginkgo leaf extract
Object, 0.4g/L potassium dihydrogen phosphate, 20g/L lignocellulosic, 0.3g/L magnesium sulfate, 20g/L sugar, 2g/L yeast powder.The shellfish
Shell powder passes through 560 ~ 575 DEG C, and the internal environment of 0.03 atmospheric pressure calcines 1h, is then polished into the hydroxide of sprinkling 10% after powder
Sodium solution, and the 5h in 200 DEG C of temperature, then with phosphorus acid for adjusting pH value to 6-6.5.
2, prepared by oil tea mushroom activated liquid strain
1）Oil tea massee fruiting bodies activation culture, the closed culture in activation medium by oil tea massee fruiting bodies, until there is mycelia
Ball, culture environment are low pressure hyperoxia, and air pressure is 0.2 atmospheric pressure, oxygen concentration 24%；
2）Fermented and cultured, by step 1）Mycelium pellet be connected in the fermentation broth in fermentor and cultivate 3-5 days, and obtain
Oil tea mushroom activated liquid strain, 28 DEG C of fermentation condition pH5.2, temperature.
Embodiment two
The preparation method for present embodiments providing a kind of oil tea mushroom activated liquid strain, includes the following steps：
1, culture medium prepares
1）In activation medium：45g/L potato juice, 20g/L agar and 10g/L ginkgo biloba p.e, ginkgo biloba p.e contain
There are the GINKGO BILOBA EXTRACT of 20-30% and the lactones of 2-8%, ginkgo biloba p.e is purchased from one hundred Si Te Biotechnology Co., Ltd of Xi'an.
2）Fermentation broth：20g/L wheat flour, 20g/L carrot meal, 45g/L conch meal, 0.2g/L ginkgo leaf mention
Take object, 0.5g/L potassium dihydrogen phosphate, 10g/L lignocellulosic, 0.4g/L magnesium sulfate, 10g/L sugar, 2g/L yeast powder.Described
Conch meal passes through 560 ~ 575 DEG C, and the internal environment of 0.08 atmospheric pressure calcines 3h, sprays 10-15%'s after being then polished into powder
Sodium hydroxide solution, and 3 ~ 5h in 200 ~ 300 DEG C of temperature, then with phosphorus acid for adjusting pH value to 6-6.5.
2, prepared by oil tea mushroom activated liquid strain
1）Oil tea massee fruiting bodies activation culture, the closed culture in activation medium by oil tea massee fruiting bodies, until there is mycelia
Ball, culture environment are low pressure hyperoxia, and air pressure is 0.8 atmospheric pressure, oxygen concentration 30%；
2）Fermented and cultured, by step 1）Mycelium pellet be connected in the fermentation broth in fermentor and cultivate 3-5 days, and obtain
Oil tea mushroom activated liquid strain, 28-30 DEG C of fermentation condition pH5.2-5.5, temperature.
Embodiment three
The preparation method for present embodiments providing a kind of oil tea mushroom activated liquid strain, includes the following steps：
1, culture medium prepares
1）In activation medium：20g/L potato juice, 35g/L agar and 7g/L ginkgo biloba p.e, ginkgo biloba p.e contain
The GINKGO BILOBA EXTRACT of 20-30% and the lactones of 2-8%, ginkgo biloba p.e are purchased from one hundred Si Te Biotechnology Co., Ltd of Xi'an.
2）Fermentation broth：15g/L wheat flour, 27g/L carrot meal, 38g/L conch meal, 2g/L ginkgo leaf extract
Object, 0.45g/L potassium dihydrogen phosphate, 15g/L lignocellulosic, 0.33g/L magnesium sulfate, 15g/L sugar, 4g/L yeast powder.Described
Conch meal passes through 560 ~ 575 DEG C, and the internal environment of 0.03 ~ 0.08 atmospheric pressure calcines 1 ~ 3h, sprays after being then polished into powder
The sodium hydroxide solution of 10-15%, and 3 ~ 5h in 200 ~ 300 DEG C of temperature, then with phosphorus acid for adjusting pH value to 6-6.5.
2, prepared by oil tea mushroom activated liquid strain
1）Oil tea massee fruiting bodies activation culture, the closed culture in activation medium by oil tea massee fruiting bodies, until there is mycelia
Ball, culture environment are low pressure hyperoxia, and air pressure is 0.2-0.8 atmospheric pressure, oxygen concentration 24-30%；
2）Fermented and cultured, by step 1）Mycelium pellet be connected in the fermentation broth in fermentor and cultivate 3-5 days, and obtain
Oil tea mushroom activated liquid strain, 28-30 DEG C of fermentation condition pH5.2-5.5, temperature.
Test example
In order to prove technical effect of the invention, the present invention also provides test example once,
Test group：Using the method culture oil tea mushroom activated liquid strain of one ~ embodiment of embodiment three, be respectively labeled as S1, S2,
S3 group.
Control group：Using the technology culture oil tea mushroom liquid strain of patent CN201610472477.9, it is labeled as D1, is used
The technology culture oil tea mushroom liquid strain of patent CN201510587382.7, is labeled as D2.
After culture, take 1L liquid spawn respectively, first stand removal lower sediment thing, then by 3000r/min from
Heart 10min, tipping supernatant claim bacterium mud weight, concrete outcome：
| Group | Weight（g） |
| S1 | 34.5 |
| S2 | 35.6 |
| S3 | 35.2 |
| D1 | 20.1 |
| D2 | 22.3 |
By above-mentioned Experimental comparison it is found that culture technique of the invention is substantially better than the prior art.
Claims (8)
1. a kind of preparation method of oil tea mushroom activated liquid strain, it is characterised in that include the following steps：
1）Oil tea massee fruiting bodies activation culture, the closed culture in activation medium by oil tea massee fruiting bodies, until there is mycelia
Ball, culture environment are low pressure hyperoxia, and air pressure is 0.2-0.8 atmospheric pressure, oxygen concentration 24-30%；
2）Fermented and cultured, by step 1）Mycelium pellet be connected in the fermentation broth in fermentor and cultivate 3-5 days, and obtain
Oil tea mushroom activated liquid strain.
2. the preparation method of oil tea mushroom activated liquid strain as described in claim 1, it is characterised in that：The activation culture
Contain 15-45g/L potato juice, 20-40g/L agar and 4-10g/L ginkgo biloba p.e in base.
3. the preparation method of oil tea mushroom activated liquid strain as described in claim 1, it is characterised in that：The activation culture
Contain 20g/L potato juice, 35g/L agar and 7g/L ginkgo biloba p.e in base.
4. the preparation method of oil tea mushroom activated liquid strain as described in claim 1, it is characterised in that：The fermented liquid
Culture medium contains following component：10-20g/L wheat flour, 20-30g/L carrot meal, 35-45g/L conch meal, 0.2-5g/L ginkgo
Leaf extract, 0.4-0.5g/L potassium dihydrogen phosphate, 10-20g/L lignocellulosic, 0.3-0.4g/L magnesium sulfate, 10~20g/L
Sugar, 2-5g/L yeast powder.
5. the preparation method of oil tea mushroom activated liquid strain as described in claim 1, it is characterised in that：The fermented liquid
Culture medium contains following component：15g/L wheat flour, 27g/L carrot meal, 38g/L conch meal, 2g/L ginkgo biloba p.e,
0.45g/L potassium dihydrogen phosphate, 15g/L lignocellulosic, 0.33g/L magnesium sulfate, 15g/L sugar, 4g/L yeast powder.
6. the preparation method of oil tea mushroom activated liquid strain as claimed in claim 2 or 4, it is characterised in that：The ginkgo
Leaf extract contains the GINKGO BILOBA EXTRACT of 20-30% and the lactones of 2-8%.
7. such as the preparation method of oil tea mushroom activated liquid strain described in claim 5 or 6, it is characterised in that：The shell
Powder passes through 560 ~ 575 DEG C, and the internal environment of 0.03 ~ 0.08 atmospheric pressure calcines 1 ~ 3h, sprays 10-15% after being then polished into powder
Sodium hydroxide solution, and 3 ~ 5h in 200 ~ 300 DEG C of temperature, then with phosphorus acid for adjusting pH value to 6-6.5.
8. the preparation method of oil tea mushroom activated liquid strain as described in claim 1, it is characterised in that：The fermentation training
Feeding fermentation condition is pH5.2-5.5,28-30 DEG C of temperature.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN111165273A (en) * | 2020-02-26 | 2020-05-19 | 河南城建学院 | Culture method and culture medium for high-yield selenium-rich cordyceps militaris |
| CN112840944A (en) * | 2019-11-26 | 2021-05-28 | 江苏红叶福茸农业科技有限公司 | Pollution prevention and control method in shiitake mushroom cultivation process |
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| CN111165273B (en) * | 2020-02-26 | 2022-04-15 | 河南城建学院 | Culture method and culture medium for high-yield selenium-rich cordyceps militaris |
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