CN104860731A - Preparation method for bio-organic fertilizer capable of improving taste quality of cherry tomato - Google Patents
Preparation method for bio-organic fertilizer capable of improving taste quality of cherry tomato Download PDFInfo
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Abstract
The invention discloses a preparation method for a bio-organic fertilizer capable of improving the taste quality of a cherry tomato. The preparation method comprises the following steps: step 1, preparing laminarin enzymatic hydrolysate; step 2, preparing a bagasse ferment material; step 3, preparing a detoxified chicken manure ferment material; step 4, uniformly mixing the laminarin enzymatic hydrolysate, the bagasse ferment material and the detoxified chicken manure ferment material in proportion, inoculating EM inocula and carrying out secondary fermentation; and step 5, after completion of secondary fermentation, successively carrying out air drying, fragmentation, bagging and preservation. According to the method, a plurality of different microbes are used to respectively ferment a plurality of industrial and agricultural waste residues, the fermented waste residues are mixed and secondary fermentation is carried out, so the bio-organic fertilizer which can be easily utilized and absorbed by the cherry tomato is prepared; the bio-organic fertilizer does not contain chemical fertilizer, is rich in a plurality of carbohydrates, common trace elements and various beneficial microbes, can improve the taste quality of the cherry tomato, is beneficial for improving soil quality and reducing obstacles of continuous cropping, has high economic and ecological benefits and is worth wide popularization.
Description
Technical field:
The present invention relates to agricultural fertilizer field, particularly relate to a kind of preparation method of biological organic fertilizer, be specially a kind of preparation method improving the biological organic fertilizer of cherry tomato taste quality.
Background technology:
Cherry tomato (Lycapersicon esculentum Var.Cerasiforme Alef.) has another name called mini-tomato, mini tomato, little tomato, cherry tomato etc., it is annual herb plant, belong to the mutation that Solanaceae tomato belongs to, originate in the Peru of South America, the ancestors of cultivated tomato, its shape has plum shape, pyriform, oblong etc., and the color after maturation then has yellow, pink, bright red, orange etc.Cherry Tomato Fruit is bright-colored beautiful, taste sweet and sour palatability, and rich vitamin and mineral substance have nutritive value and pharmaceutical use, is suitable for being processed into whole fruit can, dry fruit, eating raw or prepared food, is well received by consumers.
Nutritive substance in ripe cherry tomato comprises soluble sugar, organic acid, Lyeopene and VITAMIN etc., although Lyeopene does not possess the activity of Va precursor, but there is anti-oxidant, anti-cancer, anticancer, important physiological function such as removing toxic substance, activating immune cell etc., therefore just day by day come into one's own.Containing the VITAMIN that multiple human body is necessary in cherry tomato, wherein the highest with Va and Vc content, and Vc content is 1.7 times of Lycopersicon esculentum.Duration of storage, the Vc content in Cherry Tomato Fruit can decline along with the prolongation of storage time, and along with fruit maturity increases, accelerated breathing, in storage environment when high temperature and oxygen abundance, the easy oxidized decomposition of Vc, Vc content loss is accelerated.
The main nutrient composition in cherry tomato is not only by soluble sugar and organic acid, also be the main flavor of cherry tomato simultaneously, sapid cherry tomato must have higher sugar and acid content, the two ratio just can present fruit good local flavor time suitable, also someone thinks after studying that Cherry Tomato Fruit local flavor depends primarily on the absolute content of soluble sugar and titratable acid, little with sugar-acid ratio relation.Another factor that cherry tomato adopts the change of after ripening process flavor is the increase of volatile matter, their concentration is directly proportional to tomato maturation, and the composition of the volatile matter of Different ripening stages is variant, scientific worker utilizes the ripening degree of this evaluating characteristics tomato.
Chinese invention patent CN103964958A discloses the preparation method of the special organic substrate of a kind of cherry tomato, comprise organic fermentative processing, air-dry, pulverization process, screening, clean and the steps such as wind election process process, the total nitrogen content 8-12% of obtained organic substrate, carbon content 65-72%, total sugar content 8-12%, reducing sugar content 5-9%, nicotine content 1-1.8%, content of humic acid is 7-12%, the organic substrate that this invention obtains can improve the output of cherry tomato greatly, improve quality, and effectively can reduce the generation of disease, but its cherry tomato flavor taste quality is unstable, along with the Succession cropping effect quality of plantation reduces.
Chinese invention patent CN101857469A relates to a kind of production preparation process for golf course lawn slow-release bio-organic fertilizer that continuously ferments for three times, bright high-grade golf course lawn slow-release bio-organic fertilizer cultivation factory pig manure being carried out to high effect low stain low residue that three continuous fermentable obtain after pelletization treatment again of this method.Nutritive element can be obtained complete through such three fermentable; Soil can be improved and can improve product quality again; Rhizosphere microorganisms of crops group can be improved, improve the resistant to diseases and insects of plant; The utilization of chemical fertilizer can be promoted and improve the biological organic fertilizer of chemical fertilizer utilization ratio; And contained nutritive element can discharge slowly in biological organic fertilizer, thus the lawn acting on golf course that can be more effectively lasting, the present invention to grow demand for cherry tomato on this basis, with the addition of other multiple industrial or agricultural produced by fermenting waste residues material, making through mixing Secondary Fermentation.
Summary of the invention:
The present invention is directed to prior art Problems existing, a kind of preparation method improving the biological organic fertilizer of cherry tomato taste quality is provided, the taste quality of cherry tomato can be improved, be of value to and improve soil quality, reduce Succession cropping effect and cause fruit quality decline problem, for cherry tomato, there is single-minded wholesomeness, there are high economic benefit and ecological benefits.
In order to achieve the above object, the invention provides following technical scheme:
Improve a preparation method for the biological organic fertilizer of cherry tomato taste quality, comprise the following steps:
The first step: interpolation cellulase, hemicellulase, polygalacturonase, proteolytic enzyme prepare laminarin enzymolysis liquid;
Second step: by inoculation yeast bacterium (Yeast) fermentation after the process of bagasse high pressure-temperature for bagasse fermentation material;
3rd step: photosynthetic bacteria (PSB) ferment to inoculate after fresh chicken manure sporotrichum thermophile bacterium (Sporotrichumthermophile), azotobacter chroococcum (Azotobacter chroococcum), bacillus megaterium (Bacillus megatherium), colloid sample genus bacillus (Bacillus mucilaginosus) again fermentation obtain chicken manure fermenting material;
4th step: laminarin hydrolyzed solution, bagasse fermentation material and chicken manure fermenting material are mixed rear inoculation EM microbial inoculum Secondary Fermentation according to weight ratio 1:5:20;
5th step: Secondary Fermentation terminates rear air-dry fragmentation pack and preserves.
Preferably, the preparation process of described the first step laminarin enzymolysis liquid is:
Water Fresh Laminaria Japonica being added 7-8 times of quality smashs into sea-tangle slurry to pieces, regulate sea-tangle slurry pH to be 6.0, add the cellulase (2000U/g) of kelp paste weight 1.2%, 0.8% hemicellulase (2000U/g), 1.0% polygalacturonase (2000U/g), 1.5% proteolytic enzyme (2000U/g) mix after under 45 DEG C of bath temperature conditions enzymolysis 5-6 hour obtain laminarin enzymolysis liquid.
Preferably, in described second step, by bagasse pressure kettle boiling 30 minutes at 121 DEG C of temperature, be cooled to inoculation yeast bacterium after room temperature, inoculum size is 2.0%, fermentation time 10-12 days, and fermentation is for bagasse fermentation material.
Preferably, in described 3rd step in fresh chicken manure sprinkling per ton 2,000 hundred million photosynthetic bacterias, the fermentation completing photosynthetic bacteria for 12-15 hour is kept under intensity of illumination is 50000LUX condition, obtain chicken manure pre fermentation material, inoculate in the chicken manure pre fermentation material of every 100 parts of weight the sporotrichum thermophile bacterium of pre fermentation material quality 1.4%, 0.8% azotobacter chroococcum, the bacillus megaterium of 1.0%, the colloid sample genus bacillus of 0.5%, inoculation secondary fermentation 20-22 days, obtained chicken manure fermenting material.
Preferably, in described 4th step, the inoculum size of EM microbial inoculum is 3-4%, inoculation secondary fermentation 3-4 days.
Preferably, in described 5th step, Secondary Fermentation is air-dry after completing is 15-18% to moisture content, pulverizes 40 mesh sieves, and pack sealing is preserved.
Preferably, the addition of described interpolation yeast, photosynthetic bacteria, sporotrichum thermophile bacterium, azotobacter chroococcum, bacillus megaterium, colloid sample genus bacillus and EM microbial inoculum is all with living bacteria count gauge, wherein EM microbial inoculum number of viable >=10,000,000,000/gram, all the other microbial inoculum number of viable >=500,000,000/gram.
The feature of biological organic fertilizer: 1 improves chemical fertilizer utilization ratio, and biological organic fertilizer has the effects such as fixed nitrogen, phosphorus decomposing, potassium decomposing, greatly can improve the utilization ratio of chemical fertilizer.2 improvement soil, increase soil fertility, in biological organic fertilizer, not only organic component content enriches, in addition also containing be conducive to edatope improve probiotics.After soil application biological organic fertilizer, add the content of the nutritive substances such as the organic matter of soil, nitrogen, phosphorus, potassium to a great extent, phosphorus decomposing in fertilizer, potassium solubilizing bacteria play its phosphorus decomposing, potassium decomposing effect in soil, phosphoric in soil and potassium element are discharged, adds the available content of mineral substances of plant in soil.On the other hand, because biological organic fertilizer is applied in soil, add the organic content in soil, make that soil becomes loose, nutrient laden, the original flora lived in soil obtains amount reproduction, thus ensure that the micro-ecological environment of soil.3 alleviate environmental pollution, and according to statistics, have 1.4 hundred million tons to be burned in the agricultural crop straw of more than 600,000,000 ton that China produces every year, the burning of stalk not only causes the waste of organic substance, also creates atmospheric pollution.These discarded stalks are used and are fermented into biological organic fertilizer, huge economic benefit and the protection of air will be produced to agricultural.Use biological organic fertilizer can improve soil quality and soil microenvironment.4 improve crop quality, and the multiple nutritional components that biological organic fertilizer is rich in imparts the special fertilizer efficiency of fertilizer, proves after deliberation, and biological organic fertilizer can improve color and luster, the quality such as mouthfeel and growth vigor of crop.5 alleviate crop pests, and one is the bacteriostatic action of biological organic fertilizer self, and two is effect of biological organic fertilizer on crops growth, antibacterial, disease-resistant promoter action.
EM bacterium (Effective Microorganisms) is made up of about 80 kinds of microorganisms, and EM bacterium is taught nineteen eighty-two by the ratio good photograph husband of university of Japanese Ryukyu and studies successfully, puts goods on the market the eighties.EM bacterium is based on 10 micro-life bacteria preparations of one belonging to more than 80 microorganisms and be composited of photosynthetic bacterium, milk-acid bacteria, yeast and actinomycetes.The mechanism of action is the competition forming EM bacterium and pathogenic micro-organism contention nutrition, due to EM bacterium very easily survival and reproduction in soil, so the ecologic niche in soil comparatively fast and stably can be occupied, form the advantage group of useful microbial bacteria, thus control the breeding of pathogenic micro-organism and the invasion and attack to crop, be the developing direction of the ecological agriculture, be more conducive to the Sustainable development of agricultural.
Bagasse is a kind of byproduct of sugaring, and be the slag dregs of rice after sugarcane squeezes sugar, protein content and heat are all lower.Bagasse generally contains dry-matter 90-92%, crude protein 2.0%, robust fibre 44-46%, crude fat 0.7%, nitrogen-free extract 42%, coarse ash 2-3%.
Beneficial effect of the present invention is:
1. the inventive method utilizes the mixing after multiple industrial or agricultural waste residue of fermenting respectively of multiple different microorganisms to carry out Secondary Fermentation, prepare and be conducive to the biological organic fertilizer that cherry tomato utilizes absorption, this fertilizer does not add chemical fertilizer composition, be rich in multiple sugar, normal trace element and various beneficial microorganism, the taste quality of cherry tomato can be improved, there is high economy, should be widely promoted.
2. the present invention contains multiple effective microbe, retain the good function characteristic that general fertilizer has, can promote cherry tomato plant to nutritive element absorb and tangerine resists the pathogenic effects of some pathogenic micro-organism, alleviate the disease and pest in cherry tomato planting process.
3. the present invention improves the taste quality of cherry tomato by the mode of application of organic fertilizers, avoid because change gene is made better products and cause everybody about the fear of transgenation, simultaneously the present invention is of value to and improves soil quality, reduce the cherry tomato quality decline problem that Succession cropping effect causes, there is higher ecological benefits and economic benefit
Embodiment:
Be described in detail embodiments of the invention below, the present embodiment is implemented under premised on invention technical scheme, give detailed embodiment and concrete operating process, but protection scope of the present invention is not limited to following embodiment.The experimental program of unreceipted actual conditions in embodiment, the condition that conveniently condition or manufacturers advise usually is implemented.
Embodiment one
Improve a preparation method for the biological organic fertilizer of cherry tomato taste quality, comprise the following steps:
The first step: prepare laminarin enzymolysis liquid:
Water Fresh Laminaria Japonica being added 7 times of quality smashs into sea-tangle slurry to pieces, regulate sea-tangle slurry pH to be 6.0, add the cellulase (2000U/g) of kelp paste weight 1.2%, 0.8% hemicellulase (2000U/g), 1.0% polygalacturonase (2000U/g), 1.5% proteolytic enzyme (2000U/g) mix after under 45 DEG C of bath temperature conditions enzymolysis within 5 hours, obtain laminarin enzymolysis liquid.
Second step: prepare bagasse fermentation material:
By bagasse pressure kettle boiling 30 minutes at 121 DEG C of temperature, be cooled to inoculation yeast bacterium after room temperature, inoculum size is 2.0%, and fermentation time 10 days, fermentation is for bagasse fermentation material.
3rd step: prepare chicken manure fermenting material:
Sprinkling per ton 2,000 hundred million photosynthetic bacterias in fresh chicken manure, the fermentation completing photosynthetic bacteria for 12 hours is kept under intensity of illumination is 50000LUX condition, obtain chicken manure pre fermentation material, inoculate in the chicken manure pre fermentation material of every 100 parts of weight the sporotrichum thermophile bacterium of pre fermentation material quality 1.4%, 0.8% azotobacter chroococcum, the bacillus megaterium of 1.0%, the colloid sample genus bacillus of 0.5%, inoculation secondary fermentation 20 days, obtained chicken manure fermenting material.
4th step: inoculation EM microbial inoculum Secondary Fermentation:
Laminarin enzymolysis liquid, bagasse fermentation material and chicken manure fermenting material are mixed rear inoculation EM microbial inoculum according to weight ratio 1:5:20, and inoculum size is 3%, and inoculation secondary fermentation 3 days, carries out Secondary Fermentation.
5th step: air-dry after Secondary Fermentation completes is 15% to moisture content, pulverizes 40 mesh sieves, pack sealing is preserved.
Embodiment two
Improve a preparation method for the biological organic fertilizer of cherry tomato taste quality, comprise the following steps:
The first step: prepare laminarin enzymolysis liquid:
Water Fresh Laminaria Japonica being added 8 times of quality smashs into sea-tangle slurry to pieces, regulate sea-tangle slurry pH to be 6.0, add the cellulase (2000U/g) of kelp paste weight 1.2%, 0.8% hemicellulase (2000U/g), 1.0% polygalacturonase (2000U/g), 1.5% proteolytic enzyme (2000U/g) mix after under 45 DEG C of bath temperature conditions enzymolysis within 6 hours, obtain laminarin enzymolysis liquid.
Second step: prepare bagasse fermentation material:
By bagasse pressure kettle boiling 30 minutes at 121 DEG C of temperature, be cooled to inoculation yeast bacterium after room temperature, inoculum size is 2.0%, and fermentation time 12 days, fermentation is for bagasse fermentation material.
3rd step: prepare chicken manure fermenting material:
Sprinkling per ton 2,000 hundred million photosynthetic bacterias in fresh chicken manure, the fermentation completing photosynthetic bacteria for 15 hours is kept under intensity of illumination is 50000LUX condition, obtain chicken manure pre fermentation material, inoculate in the chicken manure pre fermentation material of every 100 parts of weight the sporotrichum thermophile bacterium of pre fermentation material quality 1.4%, 0.8% azotobacter chroococcum, the bacillus megaterium of 1.0%, the colloid sample genus bacillus of 0.5%, inoculation secondary fermentation 22 days, obtained chicken manure fermenting material.
4th step: inoculation EM microbial inoculum Secondary Fermentation:
Laminarin enzymolysis liquid, bagasse fermentation material and chicken manure fermenting material are mixed rear inoculation EM microbial inoculum according to weight ratio 1:5:20, and inoculum size is 4%, and inoculation secondary fermentation 4 days, carries out Secondary Fermentation.
5th step: air-dry after Secondary Fermentation completes is 18% to moisture content, pulverizes 40 mesh sieves, pack sealing is preserved.
Embodiment three
Improve a preparation method for the biological organic fertilizer of cherry tomato taste quality, comprise the following steps:
The first step: prepare laminarin enzymolysis liquid:
Water Fresh Laminaria Japonica being added 7 times of quality smashs into sea-tangle slurry to pieces, regulate sea-tangle slurry pH to be 6.0, add the cellulase (2000U/g) of kelp paste weight 1.2%, 0.8% hemicellulase (2000U/g), 1.0% polygalacturonase (2000U/g), 1.5% proteolytic enzyme (2000U/g) mix after under 45 DEG C of bath temperature conditions enzymolysis within 6 hours, obtain laminarin enzymolysis liquid.
Second step: prepare bagasse fermentation material:
By bagasse pressure kettle boiling 30 minutes at 121 DEG C of temperature, be cooled to inoculation yeast bacterium after room temperature, inoculum size is 2.0%, and fermentation time 10 days, fermentation is for bagasse fermentation material.
3rd step: prepare chicken manure fermenting material:
Sprinkling per ton 2,000 hundred million photosynthetic bacterias in fresh chicken manure, the fermentation completing photosynthetic bacteria for 15 hours is kept under intensity of illumination is 50000LUX condition, obtain chicken manure pre fermentation material, inoculate in the chicken manure pre fermentation material of every 100 parts of weight the sporotrichum thermophile bacterium of pre fermentation material quality 1.4%, 0.8% azotobacter chroococcum, the bacillus megaterium of 1.0%, the colloid sample genus bacillus of 0.5%, inoculation secondary fermentation 21 days, obtained chicken manure fermenting material.
4th step: inoculation EM microbial inoculum Secondary Fermentation:
Laminarin enzymolysis liquid, bagasse fermentation material and chicken manure fermenting material are mixed rear inoculation EM microbial inoculum according to weight ratio 1:5:20, and inoculum size is 4%, and inoculation secondary fermentation 3 days, carries out Secondary Fermentation.
5th step: air-dry after Secondary Fermentation completes is 17% to moisture content, pulverizes 40 mesh sieves, pack sealing is preserved.
Embodiment four
Improve a preparation method for the biological organic fertilizer of cherry tomato taste quality, comprise the following steps:
The first step: prepare laminarin enzymolysis liquid:
Water Fresh Laminaria Japonica being added 8 times of quality smashs into sea-tangle slurry to pieces, regulate sea-tangle slurry pH to be 6.0, add the cellulase (2000U/g) of kelp paste weight 1.2%, 0.8% hemicellulase (2000U/g), 1.0% polygalacturonase (2000U/g), 1.5% proteolytic enzyme (2000U/g) mix after under 45 DEG C of bath temperature conditions enzymolysis within 5 hours, obtain laminarin enzymolysis liquid.
Second step: prepare bagasse fermentation material:
By bagasse pressure kettle boiling 30 minutes at 121 DEG C of temperature, be cooled to inoculation yeast bacterium after room temperature, inoculum size is 2.0%, and fermentation time 11 days, fermentation is for bagasse fermentation material.
3rd step: prepare chicken manure fermenting material:
Sprinkling per ton 2,000 hundred million photosynthetic bacterias in fresh chicken manure, the fermentation completing photosynthetic bacteria for 13 hours is kept under intensity of illumination is 50000LUX condition, obtain chicken manure pre fermentation material, inoculate in the chicken manure pre fermentation material of every 100 parts of weight the sporotrichum thermophile bacterium of pre fermentation material quality 1.4%, 0.8% azotobacter chroococcum, the bacillus megaterium of 1.0%, the colloid sample genus bacillus of 0.5%, inoculation secondary fermentation 21 days, obtained chicken manure fermenting material.
4th step: inoculation EM microbial inoculum Secondary Fermentation:
Laminarin enzymolysis liquid, bagasse fermentation material and chicken manure fermenting material are mixed rear inoculation EM microbial inoculum according to weight ratio 1:5:20, and inoculum size is 3%, and inoculation secondary fermentation 4 days, carries out Secondary Fermentation.
5th step: air-dry after Secondary Fermentation completes is 17% to moisture content, pulverizes 40 mesh sieves, pack sealing is preserved.
Add described in embodiment one to four yeast, photosynthetic bacteria, sporotrichum thermophile bacterium, azotobacter chroococcum, bacillus megaterium, colloid sample genus bacillus and EM microbial inoculum addition all with living bacteria count gauge, wherein EM microbial inoculum number of viable >=10,000,000,000/gram, all the other microbial inoculum number of viable >=500,000,000/gram.Use according to described specification sheets using method after purchase, addition is converted into living bacteria count amount to be added, EM microbial inoculum number of viable >=20,000,000,000 such as bought/gram, then effective viable bacteria content of this high vigor bacteria preparation is 2 times of 10,000,000,000/gram EM bacteria preparation, then its addition is 0.5 times of 10,000,000,000/gram EM bacteria preparation.
Planting experiment
Test materials is cherry tomato, and kind is ruby.
Test site is Yulin city, greenhouse gardening, and be loessal soil for examination soil, field capacity is 260g/kg.Soil weight 1.37g/cm3 (0-40cm soil layer), soil organic matter content 9.56g/kg, total nitrogen content 0.62g/kg, content of tatal phosphorus 0.79g/kg, full potassium content 5.65g/kg, alkali-hydrolyzable nitrogen content 24.05mg/kg, available phosphorus contents 26.76mg/kg, quick-acting potassium content 132.48mg/kg.Preceding crop is Chinese cabbage.
18 pieces are divided on above-mentioned soil, random three pieces form one group, be divided into into six groups, test group one to four uses the biological organic fertilizer of the raising cherry tomato taste quality that embodiment one prepares to cubic method respectively as base manure, amount of application is 40,000 grams/acre, other two groups adopt conventional fertilizer application type of rearing to cultivate, are denoted as control group one, two.
The red ripe phase is chosen that size is basically identical immediately, regular shape, to measure without disease and pest, the fruit quality that has no mechanical damage after gathering.
After harvesting this stubble, continue results one batch this kind cherry tomato at original soil top, Cultivate administration and fertilizing, watering mode identical with first batch.
Quality determination method:
Vc content adopts molybdenum blue colorimetric method to measure, and Soluble adhesion molecule adopts phynol method to measure, NaOH titration measuring organic acid content, Abbe refractometer measures soluble solid content, oven drying method measures fruit dry matter content, and sensory evaluation method gives taste quality marking, full marks 10 points.
Experimental result:
Table one: first batch of ruby Cherry Tomato Fruit quality evaluation
Experimental result represents in mean value ± variance mode, and according to table one, we can find out that first batch of ruby cherry tomato is in dry matter content, soluble solid, vitamins C and soluble sugar and organic acid content, and experimental group is all higher than control group.
Table two: second batch of ruby Cherry Tomato Fruit quality evaluation
Experimental result represents in mean value ± variance mode, and according to table two, we can find out that second batch of ruby cherry tomato is in dry matter content, soluble solid, vitamins C and soluble sugar and organic acid content, and experimental group is all higher than control group.And comparatively first batch, advantage is more obvious, and between experimental group first batch second batch, gap is less, control group first batch is larger with gap between second batch.
Table three: ruby cherry tomato sensory evaluation method is to mouthfeel sensory assessment result.
Experimental result represents in mean value ± variance mode, though according to table three we can find out test group comparatively control group first batch or the second crops for rotation sense evaluation and mouthfeel comprehensive grading all more excellent, and second batch can not be reduced quality, there are higher economic worth and ecological benefits.
Above content is only better embodiment of the present invention, and for those of ordinary skill in the art, according to thought of the present invention, all will change in specific embodiments and applications, this description should not be construed as limitation of the present invention.
Claims (7)
1. improve a preparation method for the biological organic fertilizer of cherry tomato taste quality, it is characterized in that, comprise the following steps:
The first step: interpolation cellulase, hemicellulase, polygalacturonase, proteolytic enzyme prepare laminarin enzymolysis liquid;
Second step: by inoculation yeast bacterium fermentation after the process of bagasse high pressure-temperature for bagasse fermentation material;
3rd step: photosynthetic bacteria ferment to inoculate after fresh chicken manure sporotrichum thermophile bacterium, azotobacter chroococcum, bacillus megaterium, colloid sample genus bacillus again fermentation obtain chicken manure fermenting material;
4th step: laminarin hydrolyzed solution, bagasse fermentation material and chicken manure fermenting material are mixed rear inoculation EM microbial inoculum Secondary Fermentation according to weight ratio 1:5:20;
5th step: Secondary Fermentation terminates rear air-dry fragmentation pack and preserves.
2. a kind of preparation method improving the biological organic fertilizer of cherry tomato taste quality according to claim 1, is characterized in that, the preparation process of described the first step laminarin enzymolysis liquid is:
Water Fresh Laminaria Japonica being added 7-8 times of quality smashs into sea-tangle slurry to pieces, regulate sea-tangle slurry pH to be 6.0, add the cellulase (2000U/g) of kelp paste weight 1.2%, 0.8% hemicellulase (2000U/g), 1.0% polygalacturonase (2000U/g), 1.5% proteolytic enzyme (2000U/g) mix after under 45 DEG C of bath temperature conditions enzymolysis 5-6 hour obtain laminarin enzymolysis liquid.
3. a kind of preparation method improving the biological organic fertilizer of cherry tomato taste quality according to claim 1, it is characterized in that: in described second step, by bagasse pressure kettle boiling 30 minutes at 121 DEG C of temperature, be cooled to inoculation yeast bacterium after room temperature, inoculum size is 2.0%, fermentation time 10-12 days, fermentation is for bagasse fermentation material.
4. a kind of preparation method improving the biological organic fertilizer of cherry tomato taste quality according to claim 1, it is characterized in that: in described 3rd step in fresh chicken manure sprinkling per ton 2,000 hundred million photosynthetic bacterias, the fermentation completing photosynthetic bacteria for 12-15 hour is kept under intensity of illumination is 50000LUX condition, obtain chicken manure pre fermentation material, the sporotrichum thermophile bacterium of pre fermentation material quality 1.4% is inoculated in the chicken manure pre fermentation material of every 100 parts of weight, the azotobacter chroococcum of 0.8%, the bacillus megaterium of 1.0%, the colloid sample genus bacillus of 0.5%, inoculation secondary fermentation 20-22 days, obtained chicken manure fermenting material.
5. a kind of preparation method improving the biological organic fertilizer of cherry tomato taste quality according to claim 1, is characterized in that: in described 4th step, and the inoculum size of EM microbial inoculum is 3-4%, inoculation secondary fermentation 3-4 days.
6. a kind of preparation method improving the biological organic fertilizer of cherry tomato taste quality according to claim 1, is characterized in that: in described 5th step, Secondary Fermentation is air-dry after completing is 15-18% to moisture content, pulverizes 40 mesh sieves, and pack sealing is preserved.
7. a kind of preparation method improving the biological organic fertilizer of cherry tomato taste quality according to claim 1, it is characterized in that: the addition of described interpolation yeast, photosynthetic bacteria, sporotrichum thermophile bacterium, azotobacter chroococcum, bacillus megaterium, colloid sample genus bacillus and EM microbial inoculum is all with living bacteria count gauge, wherein EM microbial inoculum number of viable >=10,000,000,000/gram, all the other microbial inoculum number of viable >=500,000,000/gram.
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| CN109777740A (en) * | 2019-01-02 | 2019-05-21 | 青岛农业大学 | One plant growth addictive preparation method |
| CN113767818A (en) * | 2021-09-10 | 2021-12-10 | 华南农业大学 | Method for effectively improving glossiness of cabbage heart and application thereof |
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| CN105330471A (en) * | 2015-10-31 | 2016-02-17 | 颍上县润海畜禽养殖有限公司 | Fertilizer special for black tomatoes |
| CN109777740A (en) * | 2019-01-02 | 2019-05-21 | 青岛农业大学 | One plant growth addictive preparation method |
| CN113767818A (en) * | 2021-09-10 | 2021-12-10 | 华南农业大学 | Method for effectively improving glossiness of cabbage heart and application thereof |
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Application publication date: 20150826 |