RU99115170A - Новые гены, кодирующие деацетилазы аминокислот со специфичностью к n-ацетил-l-фосфинотрицину, их выделение и применение - Google Patents

Новые гены, кодирующие деацетилазы аминокислот со специфичностью к n-ацетил-l-фосфинотрицину, их выделение и применение

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Publication number
RU99115170A
RU99115170A RU99115170/13A RU99115170A RU99115170A RU 99115170 A RU99115170 A RU 99115170A RU 99115170/13 A RU99115170/13 A RU 99115170/13A RU 99115170 A RU99115170 A RU 99115170A RU 99115170 A RU99115170 A RU 99115170A
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Russia
Prior art keywords
dna molecule
dsm
acetyl
plant
molecule according
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RU99115170/13A
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English (en)
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RU2205219C2 (ru
Inventor
Клаус БАРЧ
Гвидо КРИТЕ
Инге БРОЕР
Альфред ПЮЛЕР
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Хехст Шеринг Агрево ГмбХ
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Priority claimed from DE19652284A external-priority patent/DE19652284A1/de
Application filed by Хехст Шеринг Агрево ГмбХ filed Critical Хехст Шеринг Агрево ГмбХ
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1. Молекула ДНК, кодирующая белок, обладающий биологической активностью N-ацетил-фосфинотрициндеацетилазы, выбранная из группы, состоящей из:
а) молекул ДНК, которые могут быть выделены из вида Stenotrophomonas, депонированного под DSM No. DSM 9734 или их фрагментов или производных, и
b) молекул ДНК, которые могут быть выделены из Comamonas acidovorans, депонированного под DSM No. DSM 11070 или их фрагментов или производных.
2. Выделенная молекула ДНК по п.1, кодирующая белок, обладающий биологической активностью N-ацетил-фосфинотрициндеацетилазы и имеющий аминокислотную последовательность SEQ ID NO : 4.
3. Выделенная молекула ДНК по п.2, включающая нуклеотидную последовательность, обозначенную как SEQ ID NO : 3, или последовательность, являющуюся производной от данной последовательности с учетом вырожденности генетического кода.
4. Выделенная молекула ДНК по п.1, отличающаяся тем, что молекула ДНК выделена из Comamonas, депонированного под DSM No. DSM 11070
5. Выделенная молекула ДНК по п.1, отличающаяся тем, что молекула ДНК выделена из Stenotrophomonas, депонированного под DSM No. DSM 9734.
6. Способ получения растений с индуцируемо разрушаемыми частями, включающий
а) трансформацию клеток растений геном деацетилазы, содержащим последовательность ДНК по п.1, кодирующую белок, обладающий биологической активностью деацетилазы под контролем промотора, направляющего экспрессию в отдельных частях растения, и
b) регенерацию указанного растения из указанных клеток растения, причем указанные растения имеют части, которые могут быть индуцируемо разрушены путем обработки N-ацетил-РРТ.
7. Способ по п.6, отличающийся тем, индуцируемые мужские стерильные или женские стерильные растения получают путем специфической экспрессии указанного гена деацетилазы.
8. Молекула ДНК по п.1, встроенная в клетку растения.
9. Молекула ДНК по п.1, встроенная в растение. Нуклеотидные полследовательности приведены в графической части.
RU99115170/13A 1996-12-16 1997-12-03 Молекула днк, кодирующая белок, обладающий активностью n-ацетил-фосфинотрицин-деацетилазы, и способ получения растений с индуцируемо-разрушаемыми частями RU2205219C2 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19652284.6 1996-12-16
DE19652284A DE19652284A1 (de) 1996-12-16 1996-12-16 Neue Gene codierend für Aminosäure-Deacetylasen mit Spezifität für N-Acetyl-L-Phosphinothricin, ihre Isolierung und Verwendung

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RU99115170A true RU99115170A (ru) 2001-06-10
RU2205219C2 RU2205219C2 (ru) 2003-05-27

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US (1) US6177616B1 (ru)
EP (1) EP0942965B1 (ru)
JP (1) JP2001506130A (ru)
CN (1) CN1171993C (ru)
AT (1) ATE377072T1 (ru)
AU (1) AU727831B2 (ru)
BR (1) BR9714513A (ru)
CA (1) CA2275134A1 (ru)
CZ (1) CZ216699A3 (ru)
DE (2) DE19652284A1 (ru)
ES (1) ES2296318T3 (ru)
HU (1) HUP0000396A3 (ru)
NZ (1) NZ335852A (ru)
PL (2) PL193522B1 (ru)
RU (1) RU2205219C2 (ru)
TR (1) TR199901332T2 (ru)
WO (1) WO1998027201A2 (ru)
ZA (1) ZA9711146B (ru)

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CA1041447A (en) * 1974-01-23 1978-10-31 Tadashiro Fujii Production of 7-amino-cephem compounds by comamonas and pseudomonas
DE4126414A1 (de) * 1991-08-09 1993-02-11 Hoechst Ag Deacetylasegene zur erzeugung von phosphinothricin oder phosphinothricyl-alanyl-alanin, verfahren zu ihrer isolierung und ihre verwendung
DE4308061A1 (de) * 1993-03-13 1994-09-15 Hoechst Ag Neue Aminosäure-Deacetylase mit Spezifität für L-N-Acetyl-Phosphinothricin, ihre Herstellung und Verwendung
DE19639463A1 (de) * 1996-09-26 1998-04-02 Hoechst Schering Agrevo Gmbh Verfahren zur Herstellung weiblich steriler Pflanzen

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