CN1171993C - 编码对n-乙酰基-l-膦丝菌素具有特异性的氨基酸脱乙酰酶的新基因,其分离和应用 - Google Patents
编码对n-乙酰基-l-膦丝菌素具有特异性的氨基酸脱乙酰酶的新基因,其分离和应用 Download PDFInfo
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- CN1171993C CN1171993C CNB971806055A CN97180605A CN1171993C CN 1171993 C CN1171993 C CN 1171993C CN B971806055 A CNB971806055 A CN B971806055A CN 97180605 A CN97180605 A CN 97180605A CN 1171993 C CN1171993 C CN 1171993C
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- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
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Abstract
Description
植物 | 点滴试验,用: 粗提取液中的脱乙酰酶活性N-乙酰基-D, 1mg/ml 0.4mg/ml 0.1mg/ml [%L-PPT]1L-PPT 4mg/ml | |||||
观察结果(叶片:) | ||||||
对照 | - | - | - | - | 0 | |
pPCVK9 | +++ | + | - | - | 14.2 | |
pPCVK14 | 卷曲 | + | + | - | - | 12.1 |
pPCVK15 | 卷曲 | ++ | + | + | - | 36.0 |
pPCVK16 | 卷曲 | + | + | - | - | 4.7 |
pPCVK17 | 卷曲 | + | + | - | - | 4.3 |
植物 | 不育花朵以及花粉减少花朵的数目 | 花药中的脱乙酰酶活性[%L-PPT]1 | |
处理过的 | 未处理过的 | ||
对照 | 0 | 0 | 0 |
pPCVT14 | 18 | 0 | 70.0 |
pPCVT15 | 0 | 0 | 13.8 |
pPCT16 | 10 | 0 | 20.4 |
基因 | 生物 | 功能 | 相同氨基酸 | 保守氨基酸 |
n.n | 空肠弯曲杆菌 | 马尿酸水解酶(383AS) | 43.1% | 60.0% |
deac2 | 嗜热脂肪芽孢杆菌 | N-酰基-L-酰氨基水解酶(370AS) | 37.0% | 49.5% |
N-乙酰基-鸟氨酸(25mM) | N-乙酰基-苯丙氨酸(25mM) | N-乙酰基-膦丝菌素(12.5mM) (25mM) (50mM) | ||
5860nM | <5nM | 8.0nM | 20.0nM | 44.7nM |
I.微生物标识 | |
由保藏者提供的标号3010 | 由国际保藏机构公布的保藏号DSM 9734 |
II.科学说明和/或所建议的分类名称 | |
条目I中标识的微生物还附有()科学说明()所建议的分类名称(若有则打叉) | |
III.收到或接收 | |
本国际保藏机构于1995年2月14日<初始保藏日>1接受了以上条目1中所标识的微生物 | |
IV.接受转换的请求 | |
本国际保藏机构于 (初始保藏日期)接受条目1中的标识的微生物并于 (接受转换请求的日期)接受根据布达佩斯条约转换初始保藏的请求 | |
V.本国际保藏机构 | |
名称:DSM-德国微生物和细胞培养物保藏有限公司地址:Mascheroder Weg 1b D-38124不伦瑞克 | 有权代表该国际保藏机构的个人或官员的签名(难以辨认的签名)日期:21-02-1995 |
I.微生物标识 | |
由保藏者提供的标号B6 | 由国际保藏机构公布的保藏号DSM 11070 |
II.科学说明和/或所建议的分类名称 | |
条目I中标识的微生物还附有()科学说明(X)所建议的分类名称(若有则打叉) | |
III.收到或接收 | |
本国际保藏机构于1996年7月12日<初始保藏日>1接受了以上条目1中所标识的微生物 | |
IV.接受转换的请求 | |
本国际保藏机构于 (初始保藏日期)接受条目1中的标识的微生物并于 (接受转换请求的日期)接受根据布达佩斯条约转换初始保藏的请求 | |
V.本国际保藏机构 | |
名称:DSM-德国微生物和细胞培养物保藏有限公司地址:Mascheroder Weg 1b D-38124不伦瑞克 | 有权代表该国际保藏机构的个人或官员的签名(难以辨认的签名)日期:16-07-1996 |
Claims (9)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE19652284A DE19652284A1 (de) | 1996-12-16 | 1996-12-16 | Neue Gene codierend für Aminosäure-Deacetylasen mit Spezifität für N-Acetyl-L-Phosphinothricin, ihre Isolierung und Verwendung |
DE19652284.6 | 1996-12-16 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN1240476A CN1240476A (zh) | 2000-01-05 |
CN1171993C true CN1171993C (zh) | 2004-10-20 |
Family
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Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CNB971806055A Expired - Fee Related CN1171993C (zh) | 1996-12-16 | 1997-12-03 | 编码对n-乙酰基-l-膦丝菌素具有特异性的氨基酸脱乙酰酶的新基因,其分离和应用 |
Country Status (18)
Country | Link |
---|---|
US (1) | US6177616B1 (zh) |
EP (1) | EP0942965B1 (zh) |
JP (1) | JP2001506130A (zh) |
CN (1) | CN1171993C (zh) |
AT (1) | ATE377072T1 (zh) |
AU (1) | AU727831B2 (zh) |
BR (1) | BR9714513A (zh) |
CA (1) | CA2275134A1 (zh) |
CZ (1) | CZ216699A3 (zh) |
DE (2) | DE19652284A1 (zh) |
ES (1) | ES2296318T3 (zh) |
HU (1) | HUP0000396A3 (zh) |
NZ (1) | NZ335852A (zh) |
PL (2) | PL193522B1 (zh) |
RU (1) | RU2205219C2 (zh) |
TR (1) | TR199901332T2 (zh) |
WO (1) | WO1998027201A2 (zh) |
ZA (1) | ZA9711146B (zh) |
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DE531716C (de) | 1930-06-08 | 1931-08-18 | Mergenthaler Setzmaschfab Gmbh | Einrichtung zum Ausrichten der Giessform gegenueber dem Giessformtraeger von Matrizensetz- und Zeilengiessmaschinen |
CA1041447A (en) * | 1974-01-23 | 1978-10-31 | Tadashiro Fujii | Production of 7-amino-cephem compounds by comamonas and pseudomonas |
DE4126414A1 (de) * | 1991-08-09 | 1993-02-11 | Hoechst Ag | Deacetylasegene zur erzeugung von phosphinothricin oder phosphinothricyl-alanyl-alanin, verfahren zu ihrer isolierung und ihre verwendung |
DE4308061A1 (de) | 1993-03-13 | 1994-09-15 | Hoechst Ag | Neue Aminosäure-Deacetylase mit Spezifität für L-N-Acetyl-Phosphinothricin, ihre Herstellung und Verwendung |
DE19639463A1 (de) | 1996-09-26 | 1998-04-02 | Hoechst Schering Agrevo Gmbh | Verfahren zur Herstellung weiblich steriler Pflanzen |
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1996
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- 1997-12-03 PL PL97334530A patent/PL193522B1/pl not_active IP Right Cessation
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- 1997-12-03 BR BR9714513A patent/BR9714513A/pt not_active Application Discontinuation
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CA2275134A1 (en) | 1998-06-25 |
EP0942965A2 (de) | 1999-09-22 |
CZ216699A3 (cs) | 1999-10-13 |
CN1240476A (zh) | 2000-01-05 |
US6177616B1 (en) | 2001-01-23 |
AU727831B2 (en) | 2001-01-04 |
PL193522B1 (pl) | 2007-02-28 |
PL334530A1 (en) | 2000-02-28 |
BR9714513A (pt) | 2000-03-21 |
AU5753598A (en) | 1998-07-15 |
ES2296318T3 (es) | 2008-04-16 |
EP0942965B1 (de) | 2007-10-31 |
DE59712890D1 (de) | 2007-12-13 |
ATE377072T1 (de) | 2007-11-15 |
HUP0000396A2 (hu) | 2000-06-28 |
WO1998027201A2 (de) | 1998-06-25 |
RU2205219C2 (ru) | 2003-05-27 |
WO1998027201A3 (de) | 1999-03-04 |
JP2001506130A (ja) | 2001-05-15 |
TR199901332T2 (xx) | 1999-09-21 |
NZ335852A (en) | 2001-06-29 |
ZA9711146B (en) | 1998-06-17 |
HUP0000396A3 (en) | 2002-02-28 |
DE19652284A1 (de) | 1998-06-18 |
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