KR970704038A - 색소체 2-옥소글루타레이트/말레이트 트랜스로케이터를 코딩하는 dna 분자(dna molecules which code for a plastid 2-oxoglutarate/malate translocator) - Google Patents

색소체 2-옥소글루타레이트/말레이트 트랜스로케이터를 코딩하는 dna 분자(dna molecules which code for a plastid 2-oxoglutarate/malate translocator) Download PDF

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KR970704038A
KR970704038A KR1019960707276A KR19960707276A KR970704038A KR 970704038 A KR970704038 A KR 970704038A KR 1019960707276 A KR1019960707276 A KR 1019960707276A KR 19960707276 A KR19960707276 A KR 19960707276A KR 970704038 A KR970704038 A KR 970704038A
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oxoglutarate
recombinant dna
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plasmid
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플뤼게 울프-인고
웨버 안드레아스
피셔 카르스텐
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로타르 빌미체르
훽스트 쉐링 아그레보 게엥베하
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Abstract

본 발명은 색소체 2-옥소글루타레이트/말레이트 트랜스로케이터를 코딩하는 DNA 분자에 관한 것으로서, 상기 DNA 분자는, 박테리아, 진균류, 유전전환성 식물 세포 및 상기 DNA 분자를 함유하는 유전전환성 식물 뿐 아니라, 특히 스피나시아 올레라세아로부터 얻어서, 색소체 2-옥소글루타레이트/말레이트 트랜스로케이터를 코딩하는 것을 특징으로 한다.

Description

색소체 2-옥소글루타레이트/말레이트 트랜스로케이터를 코딩하는 DNA 분자(DNA MOLECULES WHICH CODE FOR A PLASTID 2-OXOGLUTARATE/MALATE TRANSLOCATOR)
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
도 1은 효모에서 발현되는 재조합 2-옥소글루타레이트/말레이트 트랜스로케이터의 수송활성의 재구성을 나타낸다. 상기 도면은 에스, 폼베(S.pombe) 세포막을 함유하는 재구성 리포좀에서 〔14C〕말레이트의 흡수를 나타낸다,
도 2는 플라스미드 pEVP11-211(도 2A)및 플라스미드 pBinAR-211(도 2B)의 클로닝을 도식적으로 나타낸다.
도 3은 2-옥소글루타레이트/말레이트 트랜스로케니터 전구체 단백질을 엽록체로의 표적 및 내부 엔버로프막내에서 성숙한 단백질의 에너지-의존 삽입을 나타내는 것이다.

Claims (18)

  1. (a) 서열번호 1 로 나타난 아미노산 서열을 갖는 단백질을 코딩하는 DNA 분자; (b) 서열번호 1로 나타난 코딩 영역을 나타내는 DNA 분자; (c)(a) 또는 (b)에서 언급된 DNA 분자에 하이브리드화되는 DNA 분자; (d) (a),(b) 또는 (c)에서 언급된 DNA 분자의 서열과 비교되는 유전 코딩의 결과 퇴화된 DNA 분자 서열; 및 (e)(a),(b),(c) 또는 (d)로 언급된 분자에 상보적인 DNA 분자로 구성된 그룹에서 선택되는 것을 특징으로 하는, 색소체 2-옥소글루타레이트/말레이트 트랜스로케이터를 코딩하는 DNA 분자.
  2. 제 1항에 따른 DNA 분자를 포함하고 있는 것을 특징으로 하는 재조합 DNA 분자.
  3. 제 2 항에 있어서, 제 1 항에 따른 DNA 서열이 진핵- 또는 원핵 세포에서의 전사를 확실하게 하는 DNA서열과 연결되어 있는 것을 특징으로 하는 재조합 DNA 분자.
  4. 제 3 항에 잇어서, 2-옥소글루타레이트/말레이트 트랜스로케이터의 활성을 갖는 단백질을 코딩하는 전사가능한 mRNA의 발현을 확실하게 하는 것을 특징으로 하는 재조합 DNA 분자.
  5. 제 3 항에 있어서, 안티-센스 효과 또는 리보자임 활성에 의하여, 하나 이상의 2-옥소글루타레이트/말레이트 트랜스로케이터의 합성을 방해하는 비-전사성 RNA를 발현시키는 것을 특징으로 하는 재조합 DNA 분자.
  6. 제 3 항에 있어서, 변형된 특이성을 갖는 색소체 디카르복실레이트 트랜스로케이터를 발현시키는 것을 특징으로 하는 재조합 DNA 분자.
  7. E. coli 균주 TG1 pBSC-211 에서 DSM 9238로 기탁된 플라스미드 pBSC-211.
  8. E. coli 균주 TG1 pEVP11-211 에서 DSM 9237로 기탁된 플라스미드 pEVP11-211.
  9. E. coli 균주 TG1 pBinAR-211 에서 DSM 9239로 기탁된 플라스미드 pBinAr-211.
  10. 제 1 항에 따른 DNA 분자, 제 2 항 내지 제 6 항 중 어느 한 항에 따른 재조합 DNA 분자 똔느 제 7 항 내지 제 9 항 중 어느 한 항에 따른 플라스미드를 함유하는 것을 특징으로 하는 박테리아.
  11. 제 1 항에 따른 DNA 분자, 제 2 항 내지 제 6 항 중 어느 한 항에 따른 재조합 DNA 분자 또는 제 8 항에 따른 플라스미드를 포함하는 것을 특징으로 하는 진균류.
  12. 제 2 항 내지 제 6 항 중 어느 한 항에 따른 재조합 DNA 분자, 또는 제 9 항에 따른 플라스미드를 포함하는 것을 특징으로 하는 유전전환성 식물 세포.
  13. 제 12 항에 따른 식물세포로부터 재생가능하거나 제 12 항에 따른 식물 세포를 포함하는 것을 특징으로 하는 유전전환성 식물.
  14. 제 1 항에 따른 DNA 분자에 의하여 코딩되는 것을 특징으로 하는, 색소체 2-옥소글루타레이트/말레이트의 활성을 갖는 단백질.
  15. 디카르복실레이트 트랜스로케이터, 특히 2-옥소글루타레이트/말레이트 트랜스로케이터 또는 글루타메이트/말레이트 트랜스로케이터의 생물학적인 활성을 갖는 폴리펩티드를 코딩하는 DNA 분자를 분리할때 제 1 항에 따른 DNA 분자를 사용하는 것을 특징으로 하는 DNA 분자의 용도.
  16. 원핵성 또는 진핵성 단백질을 상기 서열을 사용하여 색소체 엔버로프 막내로, 플라스미드 스트로마내로 또는 티라코이드내로 이동시키기 위해서 표적 서열로서 사용하는 것을 특징으로 하는, 제 1 항에 따른 DNA 분자 또는 그의 일부의 용도
  17. 일부가 2-옥소글루타레이트/말레이트 운반자의 생물학적 활성을 갖는 성숙한 단백질을 코딩하는 코딩 영역을 함유하여, 다른 세포의 부분 또는 세포막 시스템을 위한 표적 서열과 결합함으로써 성숙한 단백질을 다른 부분 또는 막 시스템내로 이동시키는데 사용하는 것을 특징으로 하는, 제 1항에 따른 DNA 분자 또는 그 분자의 일부의 용도.
  18. 색소체의 내부 엔버로프막을 가로질러 디카르복실레이트 또는 글루타메이트의 수송시 억제 효과를 갖는 물질을 동정하는데 사용하는 것을 특징으로 하는, 제 1 항에 따른 DNA 분자의 용도.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019960707276A 1994-06-15 1995-06-14 색소체 2-옥소글루타레이트/말레이트 트랜스로케이터를 코딩하는 dna 분자(dna molecules which code for a plastid 2-oxoglutarate/malate translocator) KR970704038A (ko)

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DEP4420782.4 1994-06-15
DE4420782A DE4420782C1 (de) 1994-06-15 1994-06-15 DNA-Sequenz, kodierend einen 2-Oxoglutarat/Malat-Translokator, Plasmide, Bakterien, Hefen und Pflanzen enthaltend diesen Transporter
PCT/EP1995/002319 WO1995034654A1 (de) 1994-06-15 1995-06-14 Dna-moleküle, die für einen plastidären 2-oxoglutarat/malat-translokator codieren

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AU2792495A (en) 1996-01-05
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